102 results about "Rapid assay" patented technology

A microfluidic device (100) for measuring a component in a liquid sample (130). The microfluidic device (100) comprises an inlet port (102) for the liquid sample (130). The inlet port (102) comprises a capillary driven sample separation means (104) configured to separate a substantially particle-free liquid filtrate (132) from the liquid sample (130). The microfluidic device (100) comprises further a capillary channel (106) connecting the inlet port (102) and a read-out chamber (108), and configured to meter a pre-determined volume of the liquid filtrate (132). A reagent storage means (110) of the microfluidic device (100) is connected to the capillary channel (106). Th reagent storage means (110) comprises a reagent (112) and an injection means (114) configured to inject the reagent (112), wherein injecting the reagent (112) in the capillary channel (106) between the reagent storage means and the read-out chamber (108) feeds the pre- determined volume of the liquid filtrate (132) into the read-out chamber (108) to be mixed and react with the injected reagent (112) in the read-out chamber (108). The microfluidic device (100) also comprises a de-bubbling means (140) configured to remove bubbles in the reagent (112) before the reagent (112) reaches the capillary channel (106), the de-bubbling means comprising a pressure buffer (116) configured to stabilize a pressure of the reagent (112) downstream of the reagent storage means (110). The microfluidic device facilitates improved monitoring of components such as ions or psychotherapeutic drugs with precise and critical levels in the body.

The invention relates to a rapid measurement method and device for cavitation damage of a water turbine runner and computer equipment. The method comprises the following steps: acquiring point cloud data collected by a 3D laser scanner for the surface of the turbine runner; determining a cavitation erosion area according to geometric features of the point cloud data; according to the point cloud data corresponding to the cavitation erosion area, the cavitation erosion damage area, the cavitation erosion damage depth and the cavitation erosion damage volume are determined; and according to the cavitation erosion area, the cavitation erosion damage area, the cavitation erosion damage depth and the cavitation erosion damage volume, determining a cavitation erosion damage measurement result of the water turbine runner. According to the method, based on the characteristics of high precision and interference resistance of the 3D laser scanner, the area with cavitation erosion is determined by using the acquired point cloud data, the area, depth and volume of the cavitation erosion area are calculated, an accurate cavitation erosion damage measurement result of the water turbine runner is generated, the limitation of a detection environment and an operation condition on cavitation erosion damage detection is avoided, and the accuracy of the detection result is improved. And efficient cavitation damage detection is realized, so that the safety of the hydroelectric equipment is improved.

The invention relates to the field of microbial aggregate detection, in particular to a method for rapidly measuring the adhesive force of aerogenic microbial aggregates, which comprises the following steps: sample preparation: taking a proper amount of microbial aggregates to be measured, and cleaning to remove suspended solids; meanwhile, taking inlet water of the reaction device, and filtering to remove suspended matters in the inlet water; sample culture: feeding water into the microbial aggregate without the suspended matters and the reaction device, and performing sample culture; observing and imaging through a microscope: observing the bubble separation condition of the cultured sample microorganism aggregate under the microscope, collecting a bubble image through a CCD camera, and obtaining the bubble particle size in the bubble image through image processing software; and adhesive force calculation: calculating the maximum adhesive force between the microbial aggregate and the bubbles according to the critical value of the diameter of the maximum bubble staying on the microbial aggregate. According to the invention, the adhesive force between the bubbles and the aggregate can be conveniently and accurately evaluated, and the measured adhesive force is close to a real environment value.

An diagnostic assay for rapidly detecting level of expression of mRNA within low volume or low expression samples by proxy of generated cDNA. The method provides for the detection of cDNA of a diseased subject with at least one predetermined reference level cDNA characteristic of a non-diseased subject wherein a deviation of the level of expression of said cDNA in comparison with the at least one reference level cDNA allows for the diagnosis and / or prognosis of the disease.

The invention provides an on-site rapid determination method for the radioactive purity of a 226Ra-containing substance, which comprises the following steps: rapidly determining the content of 226Ra in the substance by adopting a scintillation chamber method in a non-equilibrium state of 226Ra and 222Rn, and calculating the radioactive purity of 226Ra in the product according to a decay rule of 226Ra and 222Rn. The scintillation chamber is connected with a diffuser, and the diffuser is filled with a solution containing 226Ra, wherein the volume of the solution does not exceed 1 / 2 of the volume of the diffuser. The method comprises the following steps: sealing one end of a to-be-detected diffuser filled with a solution containing 226Ra by using a spiral clamp K4, exhausting the other end of the to-be-detected diffuser for 5 minutes by using a vacuum pump at a flow rate of 1L / min, emptying 222Rn in the diffuser, and sealing and accumulating for 20 minutes by using a spiral clamp K3 after the 222Rn is completely exhausted. According to the method disclosed by the invention, the content of 226Ra in a substance is rapidly determined by using a scintillation chamber method in a non-equilibrium state of 226Ra and 222Rn, and the device is simple to build and low in cost. The method is high in accuracy, simple to operate and suitable for field measurement.

The invention relates to the technical field of consumer goods detection, and discloses a detection method for rapidly determining the content of twenty-two flame retardants in consumer goods, which comprises the following steps: S1, preparing a liquid sample; s2, putting a solid sample to be detected into a reaction tube, and adding a liquid sample; step S3, extracting; and S4, analyzing the sample solution by adopting a liquid chromatograph-mass spectrometer. The method can simultaneously detect the content of twenty-two organic flame retardants, utilizes ultrasonic extraction and LCMSMS analysis, makes up the disadvantages of gas chromatography, can rapidly determine the content of twenty-two organic flame retardants, is simple in operation and short in analysis time, and has very high test efficiency. According to the method, the repeatability is 0.3%-3.8%, the reproducibility is 1.3%-2.7%, the detection limit of the method is as low as 0.02 mg / kg, and the method completely meets the control requirements of all countries and the test requirements of customers.

The invention relates to the technical field of fiber performance test and evaluation, in particular to a method for rapidly measuring the fiber breakage rate of a fiber bundle by combining a computer and amplification equipment. The invention provides a rapid detection method of a fiber end breakage rate. The detection method comprises the following steps: S1, preparing a sample; s2, obtaining a fiber cross section; s3, automatically identifying the image; and S4, calculating the fiber end breakage rate. According to the method, a laser confocal microscope is adopted to amplify the fiber cross section and continuously photograph the fiber cross section for multiple times, computer image analysis software is adopted to automatically identify and integrate the obtained whole bundle of fiber cross sections, overlapping areas among photos are removed, and the complete and accurate fiber cross section is obtained. Metallographic software is adopted to identify and mark the cellosilk, so that the rapid and accurate measurement of the cellosilk is realized.

The invention discloses construction and activity detection methods of semisynthetic chassis cells for genetic material expansion based on vibrio natrieti. The VnDX / pET23b-PtNTT2 (66-575) cell has a relatively wide nucleoside triphosphate transport active substrate spectrum, and has recognition and transport activities on natural nucleoside triphosphate (dNTPs and NTPs), pentose modified nucleoside triphosphate (2 '-OMe-NTPs and 2'-F-NTPs), and nucleoside triphosphate (dTPT3TP, dNaMTP, dCNMOTP, TPT3TP and NaMTP) containing non-natural basic groups, and the VnDX / pET23b-PtNTT2 (66-575) cell has the recognition and transport activities on the natural nucleoside triphosphate (dNTPs and NTPs) and the pentose modified nucleoside triphosphate (2 '-OMe-NTPs and 2'-F-NTPs). By means of the transport characteristic of the nucleoside triphosphate transporter PtNTT2 (66-575) and by detecting the content of ATP transported out of cells within the same time, the relative strength of the transport activity of various nucleoside triphosphate of the PtNTT2 (66-575) is simply, conveniently and rapidly determined.

The utility model discloses a rapid determination device for the moisture content of roadbed filler, and relates to the technical field of roadbed filler detection devices. The device comprises a driving box, the top of the driving box is fixedly connected with a detection box, and the bottom of one side of the driving box is provided with a sampling plate; a cleaning assembly is arranged on one side of the detection box and comprises a cleaning cylinder, the cleaning cylinder is fixed to one side of the detection box, a detection rod is slidably connected to an inner cavity of the cleaning cylinder, and a probe is fixedly connected to the bottom end of the detection rod. According to the utility model, the three sampling grooves on the surface of the sampling plate can be used for simultaneously detecting the moisture content of roadbed fillers in different areas, and the probe can be cleaned and dried through the cleaning brush and the heater in the cleaning cylinder after each detection is completed, so that the next detection is prevented from being influenced, and after the detection is completed, a detection rod is inserted into the cleaning cylinder, so that the detection efficiency is improved. The probe extends into the sampling groove in the middle, and the detection rod is fixed by using a bolt, so that the storage is completed.

The invention belongs to the technical field of metallurgical quality inspection, and relates to a sample preparation method for reducing coal moisture loss, which comprises the following steps of: sending a coal sample to a sample preparation center within 1 hour after sampling, and immediately performing high-precision division by using a riffle to divide the sample into a moisture sample preparation sub-sample and other element sample preparation sub-samples, the subsequent treatment is directly carried out, and the traditional three-time transferring and uniform mixing procedure is cancelled; after the weight of a water sample reaches the standard through multiple times of division by a riffle, crushing and screening treatment is carried out, and finally, rapid detection is carried out by adopting an automatic all-water instrument. The problems of long storage time, large moisture loss, high detection deviation and the like in a traditional method are solved, the total moisture detection result is closer to a true value, and the accuracy and the reliability are greatly improved. Experiments show that compared with the prior art, the moisture loss rate is reduced by more than 25%, the detection efficiency is improved by 30%, and the method is suitable for high-precision rapid determination of coal total moisture.

The application discloses a cherry freshness detection device based on volatile substance concentration proportion time sequence model, which comprises a handheld detection instrument main body and a sampling bottle. The detection instrument main body comprises a micro air pump, a micro air chamber and a micro processing unit. The micro processing unit is integrated with a data acquisition module, an algorithm module and a calculation detection module. The algorithm module is integrated with concentration proportion time sequence models of various volatile substances. The calculation detection module compares the concentration data of various volatile substances and the concentration proportion time sequence models to determine the picking time and freshness of cherries. Compared with the traditional method, the application has the advantages of rapid determination, and the single test time is 4 min. Compared with artificial and single alcohol detection instrument, the application uses multiple characteristic volatile substance contents as test objects, and has higher precision and repeatability.

The present application relates to a fluorescence analysis method for rapidly determining the copper and zinc content and adhesion amount in brass-plated steel cords, and the fluorescence analysis method comprises the following steps: sample preparation, standard sample selection, instrument measurement conditions, working curve drawing, and accuracy and precision determination. The present invention arranges the samples of brass-plated steel cords or strands in an orderly manner and places them in an X-ray fluorescence analyzer to directly test the copper and zinc content, and the standard curve method to test the adhesion amount of the copper and zinc coating. The fluorescence analysis method of the present invention does not require stripping treatment, is simple to operate, and has a fast test speed; a shape correction function is introduced into the calibration curve, and the analysis results are accurate and reliable, providing a fast and accurate determination method for testing the element content and adhesion amount in the steel cord coating, solving the problem of testing the adhesion amount of steel cord samples, and meeting the fast and accurate testing needs of enterprises.

The application relates to the technical field of intelligent agriculture and plant protection, and particularly discloses a broccoli black spot early diagnosis system and method based on multi-modal machine learning fusion, which comprises a portable collection platform, a mobile experiment terminal and a server. The portable collection platform is integrated with a hyperspectral imaging camera, a chlorophyll fluorescence imager, a dark-adapted leaf clamp and a camera for sample barcode recognition, and is provided with an adjustable height support and a controllable light source to ensure the consistency of data collection conditions. The mobile experiment terminal is internally provided with a physicochemical index rapid detection module, including a portable PCR instrument for on-site nucleic acid rapid extraction and amplification and a miniature ultraviolet spectrophotometer for rapid determination of pigment content. The broccoli black spot early diagnosis system and method based on multi-modal machine learning fusion are highly integrated, portable and automated, are suitable for a field environment and provide an efficient disease diagnosis tool for non-expert users.

The application discloses an automatic analyzer for detecting aniline compounds in liquid and a determination method, and realizes automatic and rapid determination of aniline compounds in liquid samples through the design, connection and control of flow paths and structures, and the analysis processes such as automatic sampling, reagent adding, mixing reaction and spectrophotometric detection, so that reagent consumption and waste liquid discharge are reduced, and the analysis detection sensitivity, accuracy and precision are improved.

The invention provides a method for detecting the content of zinc in a lithium-zinc binary alloy strip based on ICP-OES, and belongs to the technical field of metal material component analysis. According to the method, the lithium matrix is separated by adopting a nitric acid-perchloric acid digestion system, and the stock solution is diluted by 500 times, so that the concentration of the lithium matrix can be efficiently reduced, meanwhile, the signal inhibition effect is effectively eliminated by combining lithium salt matching, and the detection precision is greatly improved. According to the method, Zn 213.856 nm analysis spectral lines are carefully selected, RF power is optimized, spectral interference of other elements can be avoided, the linear range can be expanded, and the method is suitable for rapid determination of the zinc content of 1%-20%. According to the method, the detection limit can reach 0.01%, the relative standard deviation is smaller than or equal to 1.0%, the recovery rate is 98.5%-101.2%, and high-precision detection is achieved.

The invention provides a method for determining the content of artemisinin in artemisia apiacea through rapid thin-layer analysis, application and a colorimetric card, and belongs to the technical field of substance detection. The method provided by the invention comprises the following steps: S1, mixing a to-be-detected medicinal material with a solvent, performing ultrasonic extraction, and filtering to obtain a to-be-detected solution; s2, the to-be-detected liquid obtained in the step S1 is subjected to sample application, unfolding and drying on a thin-layer plate, and a chromatography plate is obtained; s3, carrying out color development on the chromatography plate obtained in the step S2 to obtain a color-developed chromatography plate; s4, comparing and identifying the chromogenic chromatography plate obtained in the step S3 with a standard colorimetric card, and judging the value range of the artemisinin content. The method can be used for rapidly determining whether the artemisinin content in the sweet wormwood herb is qualified or not.

The present application relates to the technical field of oilfield water detection, and is a rapid detection method for metal ions contained in fracturing water, which is performed according to the following steps: measuring total conductivity of a fracturing water sample at T1, T2 and T3 temperatures, continuously adding a precipitant NaOH to the fracturing water sample, and measuring turbidity value changes of the fracturing water; when the turbidity value is stable, a turbid fracturing water liquid is obtained, and a turbidity detection value NTU of the turbid fracturing water liquid is recorded 测 , and the amount of the precipitant NaOH, while measuring conductivity of the turbid fracturing water liquid at T1, T2 and T3 temperatures; according to a series of correlation equation groups, concentrations of Na + , K + , Ca 2+ , Mg 2+ and Ba 2+ in the fracturing water sample are obtained. The present application adopts a fitting correlation equation group and a step-by-step rapid detection method, and has the advantages of rapid detection, accuracy, low cost, environmental protection, non-toxicity, simple operation, wide application range and the like.

The invention provides a method and a system for automatically and rapidly measuring trace aluminum in cold water in a high-voltage direct-current converter valve. The system comprises a masking reagent bottle, a chromogenic reagent bottle, a buffer reagent bottle, a current-carrying reagent bottle, a water sample bottle, a flow injection analyzer, a multi-channel combination module, a first reaction coil, a second reaction coil, a third reaction coil, a temperature control module and a light splitting detector, the flow injection analyzer comprises a pump A, a pump B, a sampling valve and a sampling ring; the multi-channel combination module is provided with channels A, B and C which are not communicated with one another, and the channels A, B and C are respectively provided with sampling ports a, b and c; the sampling valve is provided with channels f, g, h and i which are not communicated with one another, and the channels f, g, h and i are provided with sampling ports d and e respectively. According to the method and the system provided by the invention, the trace aluminum ions in the cold water in the high-voltage direct-current converter valve can be automatically, quickly, accurately and reliably measured, and the detection limit is 1.0 mu g / L.

This application relates to the field of environmental monitoring and indoor air quality control technology, and particularly to a device, system, and method for rapidly determining the emission characteristics of semi-volatile organic compounds (SVCs). The device includes a test chamber and a test sample. The test chamber has a sandwich structure, during which the test sample is embedded, forming a cavity with the chamber. The main body of the test chamber is equipped with an air inlet, an air outlet, and a sampling port. Air is supplied to the cavity through the air inlet, and the airflow is discharged through the air outlet. This solves the problems of long steady-state times due to large adsorption areas in other test chambers, leading to long testing cycles, and large errors in emission characteristics due to convective mass transfer coefficient errors, when determining the emission characteristics of SVCs.

This application relates to a method, apparatus, and computer equipment for rapid determination of cavitation damage in a turbine runner. The method includes: acquiring point cloud data collected by a 3D laser scanner on the surface of the turbine runner; determining the cavitation erosion region based on the geometric features of the point cloud data; determining the cavitation damage area, cavitation damage depth, and cavitation damage volume based on the point cloud data corresponding to the cavitation erosion region; and determining the cavitation damage measurement result of the turbine runner based on the cavitation erosion region, cavitation damage area, cavitation damage depth, and cavitation damage volume. This method leverages the high precision and anti-interference characteristics of the 3D laser scanner to identify cavitation erosion areas using the collected point cloud data, calculate the area, depth, and volume of the cavitation erosion region, and generate accurate cavitation damage measurement results for the turbine runner. This avoids the limitations imposed by the detection environment and operating conditions on cavitation damage detection, achieving efficient cavitation damage detection and thus improving the safety of hydroelectric equipment.

The present application relates to the field of fluid machinery, especially to a full-flow extreme power point rapid determination method and system for diesel engine fire pump set. According to the industry standard and pump set parameters, the method presets the reference parameters and solidifies the Q-N-H three-dimensional working condition mapping table to build a standardized test reference. Four test intervals are divided according to the flow, and exclusive control algorithm and safety protection logic are matched. After the equipment is preheated and stabilized, multi-source working condition data is collected through high-frequency sampling and time stamp calibration, and the real-time input power of the diesel engine is accurately calculated through sliding filter denoising, parameter normalization and multi-dimensional loss correction. Through the least square method five polynomial fitting P-Q characteristic curve, combined with the first and second derivative joint criterion, the only power maximum point in the effective flow interval is accurately calibrated. The method is accurate, fast and highly standardized, effectively improves the determination accuracy and efficiency of the extreme power point, and meets the combined rule detection requirements of the diesel engine fire pump set.

Described herein are diagnostic and control fusion protein reagents and methods for use thereof in simple rapid and inexpensive hemagglutinin assays for the detection of subject antibodies directed to the SARS-CoV-2 virus.

The application discloses a method for rapidly separating Re and PGE from an organic matter-rich geological sample. After the sample is dissolved and converted into a sample solution dissolved in 0.2M HCl, first, a cation exchange column is used to realize rapid separation of Re-PGE from a sample matrix and interfering elements (such as Cd, Zr and Hf); second, an LN resin column is used to further remove interfering elements (such as Zr and Hf) and realize mutual separation of Re and PGE. Compared with a traditional separation method, the method can obtain high-purity Re and PGE, and the mutual separation of Re and PGE enables high-precision determination of Re by using MC-ICP-MS for Re-Os dating research, and rapid determination of PGE by using Q-ICP-MS or Element-XR ICP-MS for PGE content analysis.

The invention belongs to the technical field of detection methods, and provides a method for rapidly determining the content of index components in formula granules, and the method comprises the following steps: detecting the content of the index components in formula granules to be detected by adopting a thin-layer scanning method, and calibrating the measured content of the index components; the calibration comprises the following steps: substituting the measured index component content as an independent variable into a linear regression equation to calculate a dependent variable, and taking the calculated dependent variable as the calibrated content; wherein the establishment mode of the linear regression equation comprises the following steps: carrying out linear regression by taking the content of the index component in the formula granules detected by a thin-layer scanning method as a horizontal coordinate and taking the content of the index component in the same formula granules detected by a high-performance liquid chromatography method as a vertical coordinate. According to the method, the content of the index components in the formula granules can be rapidly determined, the operation is simple and convenient, the result accuracy is high, and the method has a good application prospect.

The invention relates to a fluorescence analysis method for rapidly measuring high-sulfur copper concentrate. The fluorescence analysis method comprises the following steps: preparing a high-sulfur copper concentrate sample, grinding and screening the high-sulfur copper concentrate sample, carrying out chemical value determination, preparing a powder tabletting high-sulfur copper concentrate sample, setting conditions of an X-ray fluorescence spectrophotometer, and establishing an analysis curve of each element. According to the scheme provided by the invention, the content of Cu, Fe, S, SiO2, CaO, Pb, Zn, As, MgO, Al2O3, Sb, Bi, Ni, Sn, F and Cl in the high-sulfur copper concentrate can be accurately measured.

The invention relates to the technical field of material thermal performance detection, and discloses a heat transfer coefficient determination and dynamic monitoring system of a thermal insulation material. According to the system, through integration of a composite working condition heat source module, a multi-dimensional coupling sensor array, a real-time interference compensation unit and an LSTM intelligent prediction system, the bottlenecks of long steady-state measurement time consumption, low non-steady-state precision, lack of dynamic monitoring and incapability of predicting the trend in the prior art are broken through; self-adaptive switching between non-steady-state rapid monitoring and steady-state high-precision calibration is achieved on hardware, a coupling model of heat transfer coefficients, environmental parameters and material performance is constructed on software, rapid measurement, dynamic monitoring and long-term trend prediction of the K value of the thermal insulation material are achieved in combination with cloud data interaction, and the method is suitable for multiple scenes such as buildings, pipelines and cold chains and has a wide application prospect. And a technical support is provided for full-life-cycle energy efficiency management of the thermal insulation material.

The invention discloses a method for rapidly measuring the pH value of a trace PET probe through a spectrum method and application of the method. The method has the advantages that the using amount of a sample is extremely small, a standard spectrum curve is drawn after experimental conditions are optimized, the measuring steps are simple, the measuring result is high in accuracy and sensitivity, and meanwhile microfluidic automatic online measuring can be achieved. The PET tracer agent has the characteristics of small synthesis amount, high price, radioactivity and the like, so that the PET tracer agent has huge application value in the aspects of testing the use amount of samples, simplifying detection, realizing automatic testing and the like.

The application provides a kind of geological sample low content Re fast and accurate analysis method, comprising: collecting sample to be determined and carrying out sample crushing;Weighing sample to be determined in plastic centrifuge tube;HCl, HNO3 and Re diluent are sequentially added to the plastic centrifuge tube;The plastic centrifuge tube is covered with a bottle cap and ultrasonic oscillation is carried out, the plastic centrifuge tube is placed in an oven at 105 DEG C to 110 DEG C and heated, and low-temperature sample dissolution is carried out;After heating is completed, the plastic centrifuge tube is placed in a centrifuge for centrifugation;The supernatant is diluted and then MC-ICP-MS is used for rapid determination of Re content.The method only uses low-cost plastic centrifuge tubes for sample dissolution, reduces the detection cost by 70% under the premise of ensuring the analysis accuracy;The Re content determination method only needs one day to complete the whole Re content process, significantly improving the single sample analysis efficiency;The Re content determination method is simple, short in determination period and cost-saving.

The present invention discloses a method for rapidly determining the glass content of multi-component organic hazardous waste water-quenched slag, which matches the high temperature and gasification melting technology of multi-component organic hazardous waste and realizes the rapid determination of the glass content of water-quenched slag. The method determines the water-quenched slag particle size, color illumination L value, hardness data, moisture content, fine slag content and other indicators, combines and utilizes the BP neural network, uses the levenberg-marquardt algorithm to train the BP neural network, selects the neural network with the smallest result error among the neural networks with different hidden layer node numbers as the target neural network, and finally quickly obtains the glass content of the water-quenched slag.

This invention proposes a method for rapid determination of the content of Phellodendron amurense charcoal slices processed at different temperatures using near-infrared spectroscopy. Near-infrared spectroscopy is used to collect spectral information of Phellodendron amurense charcoal slices processed at different temperatures. Background interference is eliminated through preprocessing methods such as baseline correction, standard normal variable transformation, multivariate scattering correction, and first derivative. Combined with competitive adaptive reweighted sampling, CARS combined with iterative retention of information variables, and iterative combination optimization, a partial least squares regression quantitative prediction model is established. This model achieves accurate prediction of the content of five key components: 5-O-FQA, 3-O-FQA, berberine, berberine, and berberine, with prediction correlation coefficients all exceeding 0.90. This invention combines spectral information with chemometric methods to establish a digital content prediction model based on near-infrared spectroscopy for online monitoring of the Phellodendron amurense charcoal slice processing process. It has the advantages of objectivity, speed, non-destructive nature, and reliability, effectively solving the technical problem of lack of objectivity in existing technologies based on human judgment.