The invention provides a method for determining contents of aluminum, niobium, tungsten and chromium in a high-niobium-aluminum-titanium alloy. The method is used for determining the contents of aluminum, niobium, tungsten and chromium in the high-niobium-aluminum-titanium alloy by adopting inductively coupled plasma atomic emission spectroscopy, and specifically comprises the following steps: preparing a to-be-determined sample solution, preparing a standard solution, establishing a standard curve, determining the content of each element in the to-be-determined sample solution, and the like.According to the method disclosed by the invention, a series of standard solutions can be prepared by a matrix matching method, matrix interference and interference of inter-element spectral lines canbe reduced, the aluminum, niobium, tungsten and chromium in the high-niobium-aluminum-titanium alloy can be simultaneously analytically determined on an inductively coupled plasma optical emission spectrometer, the quantitative range can cover the detection requirement of the high-niobium-aluminum-titanium alloy, the analytical method is simple to operate, and a reliable guarantee is provided forproduction, scientific research, application and quality control of the high-niobium-aluminum-titanium alloy.