2 results about "Transport activity" patented technology

The invention discloses a transporter mutant capable of efficiently transporting non-natural nucleoside triphosphate. The molecular structure of a truncated body PtNTT2 (66-575) of a nucleoside triphosphate transporter PtNTT2 is analyzed, the PtNTT2 (66-575) is taken as an evolution starting point, key amino acid residues: 271th and 474th amino acids are selected, site-specific saturated mutation libraries are respectively constructed, and screening is carried out aiming at the transport activities of different non-natural nucleoside triphosphate, so that the site-specific saturated mutation libraries can be used for screening the transport activities of different non-natural nucleoside triphosphate. The mutant capable of efficiently transferring non-natural nucleoside triphosphate (dCNTMOTP, dNaMTP and NaMTP) is obtained by adopting the method provided by the invention. The mutant disclosed by the invention can be used for high-efficiency cell transmembrane delivery of various non-natural nucleoside triphosphates, the substrate range of nucleoside triphosphate transporter is expanded, and a foundation is laid for construction of semi-synthetic life bodies for efficiently expanding genetic letters.

This method for measuring transporter activity comprises: exposing a membrane vesicle having a vacuolar ATPase and a vesicular nucleotide transporter to an anion which is labeled with a radionuclide and is different from adenosine triphosphate, in a reaction solution containing adenosine triphosphate and magnesium ions; and measuring the transport activity of the anion into the membrane vesicle on the basis of the radiation of the radionuclide.