31 results about "Antiporter" patented technology

The present inventors successfully cloned the rice Na+ / H+ antiporter gene. It is possible to produce salt tolerant plants by using the isolated gene, or genes with equivalent functions.

The present inventors successfully cloned the rice Na+ / H+ antiporter gene. It is possible to produce salt tolerant plants by using the isolated gene, or genes with equivalent functions.

The invention discloses a recombinant bacterium producing γ-aminobutyric acid, its construction method and application. Adopt the method of the present invention to import glutamic acid decarboxylase B gene (gadB gene) into Escherichia coli mutant strain K12ΔgadABC through the expression vector pBAD / HisB. Glutamic acid: γ-aminobutyric acid antiporter is sensitive to pH, which effectively reduces the degradation of γ-aminobutyric acid and improves the yield and conversion efficiency of GABA. The transformation rate of KG01 strain was not affected by pH change, and the production of GABA was the highest, which was significantly higher than that of K12ΔgabT strain and K12 strain. Using glutamic acid with a concentration of 2M as the substrate, the KG01 strain was continuously transformed three times, and the transformation rate was above 99% each time, the yield was greater than 204g / L, and the substrate residue was low, which was convenient for downstream crystallization and purification. The gamma-aminobutyric acid produced by the genetically engineered bacteria constructed by the method of the invention has the advantages of low raw material, simple process, high production efficiency, etc., and has good industrial application prospect.

The present invention discloses an E. coli engineering bacteria producing 1,5pentanediamine through a whole cell catalysis and its application. The engineering bacteria according to the present invention, is Escherichia coli (E. coli) strain B or its derivative strains with the overexpression of a lysine decarboxylase gene and a proper expression of a lysinecadaverine antiporter gene cadB. The engineering bacteria according to the present invention is the engineering bacteria producing 1,5pentanediamine through the whole cell catalysis constructed from Escherichia coli B derivative strains, which has an overexpression of a lysine decarboxylase gene cadA and a proper expression of the lysinecadaverine antiporter gene cadB; The present invention further discloses a method of producing a 1,5pentanediamine catalyzed by the engineering bacteria, the yield and production intensity of 1,5pentanediamine in biobased production could be significantly improved through the method, hence it could be applied to mass production and convenient for extending applications.

The invention relates to the field of plant genetic engineering and provides a novel plant strong salt-tolerant gene NHXS1 obtained by a restructuring technology. A nucleotide sequence of the plant strong salt-tolerant gene NHXS1 is SEQ ID NO: 1, or a DNA sequence which has 70 to 100 percent homology with the SEQ ID NO: 1 nucleotide sequence or a DNA sequence for coding a protein sequence of SEQ ID NO: 2. Na + / H + antiporter NHXS1 colded by the gene has stronger ion transport activity than the wild-type Na + / H + antiporter AtNHX1. The invention also provides methods for construction of recombinant vectors and transgenic plants to apply the genes and proteins, and can culture a novel breed of transgenic plant with stronger salt-tolerant property or other improved biological characteristics.

The invention provides a novel plant strong salt tolerant gene AtNHXS1 acquired through DNA reshuffling technology, and the ionic transport activity of Na+ / H+ antiporter protein coded by the gene is stronger than that of wild Na+ / H+ antiporter protein ATNHX1. A nucleotide sequence of the gene as shown in SEQ ID NO:1 also comprises genes of the nucleotide sequence of sequence 1 in a sequence tablewith the homology between 70 and 100 percent, or a nucleotide sequence of an amino acid sequence of sequence 2 in a coded sequence table. The new Na+ / H+ antiporter has a protein of the amino acid sequence of sequence 2 in the sequence table, protein of the amino acid sequence of the sequence 2 with the homology between 70 and 100 percent, or protein which replaces, deletes or adds one or a plurality of amino acids in the amino acid sequence of the sequence 2 with the same homology. The invention also provides the construction of recombinant vector, the transgenic plant and other methods so asto apply the gene and the protein, thereby cultivating a novel variety of transgenic plant with strong capacity of salt tolerance or other improved biologic characters.

The invention discloses a kind of Na on the wheat plasma membrane + / H + Antiporter genes and their applications. The Na + / H + The full length of the antiporter gene is 3460bp, and after expression, the gene is located on the plasma membrane of the cell and plays a role in Na + / H + exchange function, the Na + out of the cell, and thus the present invention also discloses the use of the Na + / H + The antiporter gene constructs a plant genetic transformation vector and a method for improving the salt tolerance of the plant.

The invention discloses a tamarisk plasma membrane Na + / H + The antiporter gene and its application belong to the technical field of genetic engineering. The Tamarix plasma membrane Na + / H + The nucleotide sequence of the antiporter gene is shown in SEQ ID No.1, and the amino acid sequence of the expressed protein is shown in SEQ ID No.2. The present invention clones Tamarix plasma membrane Na for the first time from Tamarix + / H + antiporter gene, through transformation of Arabidopsis, soybean, cotton and yeast, overexpressed Tamarix plasma membrane Na + / H + Arabidopsis thaliana, soybean, cotton and yeast with antiporter gene have lower salt tolerance than untransformed or transformed soybean, cotton or Arabidopsis plasma membrane Na + / H + antiporter gene was higher, indicating that Tamarix plasma membrane Na + / H + The antiporter gene has an outstanding effect in the application of improving the salt tolerance of plants or yeast, and has important application value in the field of improving the salt tolerance breeding of plants or strains.

The invention discloses a karelinia caspica(Pall.)Less. plasma membrane Na+ / H+ reversal transport protein and an encoding gent and application thereof. An amino acid residue sequence of the kareliniacaspica(Pall.)Less. plasma membrane Na+ / H+ reversal transport protein KcSOS1 is shown in SEQ ID No.2 in a sequence table; a sequence of the gene encoding the karelinia caspica(Pall.)Less. plasma membrane Na+ / H+ reversal transport protein is shown in SEQ ID No.1. It is proved through an experiment that when a karelinia caspica(Pall.)Less. KcSOS1 gene is transferred into tobacco, the saltresistanceof the transgenic tobacco can be significantly improved. The provided karelinia caspica(Pall.)Less. KcSOS1 gene can be used for improving the salt stress resistance performance of crops, and has a wide application prospect in the field of salt tolerance inheritance improvement for the crops and fine forage grass.

Imaging agents that comprise labeled substrates of the cystine / glutamate antiporter of cells, whereby the methods of use comprise introducing the labeled agents into cells via the cystine / glutamate antiporter, which are then reduced to a labeled cysteine, and subsequently detected in the cell.

The invention belongs to the technical fields of molecular biology and genetic engineering, and discloses a novel halobacillus sodium / hydrogen antiporter gene sdmlT and identification. The novel halobacillus sodium / hydrogen antiporter gene sdmlT is a complete open reading frame ORF, and the nucleotide sequence of the ORF is shown as SEQ ID NO.1. The identification method comprises the following steps: a segment containing a salt-and-alkali-tolerant gene is first obtained; a prokaryotic expression vector containing the sdmlT gene is constructed; and the physiological function of the gene and the function of a protein coded by the gene are identified. The sdmlT gene which is obtained from halobacillus andaensis by the invention can remarkably increase the salt-and-alkali-tolerant capabilityof a salt-sensitive mutant strain KNabc of Escherichia coli; the sdmlT gene proposed by the invention enriches microbial salt-and-alkali-tolerant gene resources, can be used in the construction of highly effectively salt-and-alkali-tolerant engineering bacteria, stress-resistant crops or the like, and particularly has significant value in plants tolerant to salt or alkali stress.

The present disclosure relates in general to methods for the treatment of excitotoxicity disorders, using compositions comprising cysteamine or cystamine or salts or derivatives thereof in combination with an agent that inhibits or blocks glutamate / cystine antiporter xc−.