37 results about "Antiporter" patented technology

Imaging agents that comprise labeled substrates of the cystine / glutamate antiporter of cells, whereby the methods of use comprise introducing the labeled agents into cells via the cystine / glutamate antiporter, which are then reduced to a labeled cysteine, and subsequently detected in the cell.

The invention relates to the field of plant gene engineering, providing a buckwheat Na+ / H+ antiporter FtNHX. The buckwheat Na+ / H+ antiporter FtNHX is provided with SEQ ID No:2 amino acid residue sequence in a sequence table, or the buckwheat Na+ / H+ antiporter FtNHX is obtained by the substitution, deletion or adding of one or multiple amino acid residues by SEQ ID No: 2 in the sequence table, has the same activity with the SEQ ID No:2 amino acid residue sequence and is derived by SEQ ID No:2. The gene for coding the protein is preferably selected from a nucleotide sequence comprising the 288th-1949th bit of SEQ ID No:1, or the gene is a nucleotide sequence which has more than 90% of homology with a DNA sequence specified by the SEQ ID No:1 and codes same functional protein. The invention also provides methods for recombinant vector construction, transgenic plant and the like to apply the above gene and protein, and can cultivate new improved gene plant species with stronger salt tolerance or other biological characters.

The invention relates to the field of plant genetic engineering and provides a novel plant strong salt-tolerant gene NHXS1 obtained by a restructuring technology. A nucleotide sequence of the plant strong salt-tolerant gene NHXS1 is SEQ ID NO: 1, or a DNA sequence which has 70 to 100 percent homology with the SEQ ID NO: 1 nucleotide sequence or a DNA sequence for coding a protein sequence of SEQ ID NO: 2. Na + / H + antiporter NHXS1 colded by the gene has stronger ion transport activity than the wild-type Na + / H + antiporter AtNHX1. The invention also provides methods for construction of recombinant vectors and transgenic plants to apply the genes and proteins, and can culture a novel breed of transgenic plant with stronger salt-tolerant property or other improved biological characteristics.

The invention provides a novel CkNHX genen and excision adorn gene CkNHXn thereof. The gene stems from Caragana korshinskii Kom, its gene codes Na+ / H+ antiporter gene. The cDNA obtained by clone constructs yeast expression carrier and plant expression carrier. The yeast mutant strain vertifies salt resistance thereof by complament experiment. Moreover, a transgenic plant is also obtained. By testing salt resistance of transformed plant, transformation of the gene can make the transgenic plant with salt resistance.

The invention provides a novel plant strong salt tolerant gene AtNHXS1 acquired through DNA reshuffling technology, and the ionic transport activity of Na+ / H+ antiporter protein coded by the gene is stronger than that of wild Na+ / H+ antiporter protein ATNHX1. A nucleotide sequence of the gene as shown in SEQ ID NO:1 also comprises genes of the nucleotide sequence of sequence 1 in a sequence table with the homology between 70 and 100 percent, or a nucleotide sequence of an amino acid sequence of sequence 2 in a coded sequence table. The new Na+ / H+ antiporter has a protein of the amino acid sequence of sequence 2 in the sequence table, protein of the amino acid sequence of the sequence 2 with the homology between 70 and 100 percent, or protein which replaces, deletes or adds one or a plurality of amino acids in the amino acid sequence of the sequence 2 with the same homology. The invention also provides the construction of recombinant vector, the transgenic plant and other methods so as to apply the gene and the protein, thereby cultivating a novel variety of transgenic plant with strong capacity of salt tolerance or other improved biologic characters.

The invention belongs to the technical fields of molecular biology and genetic engineering, and discloses a novel halobacillus sodium / hydrogen antiporter gene sdmlT and identification. The novel halobacillus sodium / hydrogen antiporter gene sdmlT is a complete open reading frame ORF, and the nucleotide sequence of the ORF is shown as SEQ ID NO.1. The identification method comprises the following steps: a segment containing a salt-and-alkali-tolerant gene is first obtained; a prokaryotic expression vector containing the sdmlT gene is constructed; and the physiological function of the gene and the function of a protein coded by the gene are identified. The sdmlT gene which is obtained from halobacillus andaensis by the invention can remarkably increase the salt-and-alkali-tolerant capabilityof a salt-sensitive mutant strain KNabc of Escherichia coli; the sdmlT gene proposed by the invention enriches microbial salt-and-alkali-tolerant gene resources, can be used in the construction of highly effectively salt-and-alkali-tolerant engineering bacteria, stress-resistant crops or the like, and particularly has significant value in plants tolerant to salt or alkali stress.

The present inventors successfully cloned the rice Na+ / H+ antiporter gene. It is possible to produce salt tolerant plants by using the isolated gene, or genes with equivalent functions.

The present inventors successfully cloned the rice Na+ / H+ antiporter gene. It is possible to produce salt tolerant plants by using the isolated gene, or genes with equivalent functions.

Imaging agents are described that comprise labeled substrates capable of being introduced into cells via the cystine / glutamate antiporter. The substrates may be used for imaging or detecting oxidative stress in cells by introducing the labeled agents into cells via the cystine / glutamate antiporter and subsequent detection.

The invention discloses Na+ / H+ antiporter protein Nha-K2 and encoding gene and application thereof. The encoding gene of the Na+ / H+ antiporter protein Nha-K2 has the 571st to 2058th of the SEQ (sequence) ID NO.1 nucleotide sequence in the sequence table. The Na+ / H+ antiporter protein Nha-K2 has higher antiporter protein activity, is capable of improving haloduric performance of host bacteria after being led into the host bacteria to express, can also be transferred into crops so that transgenosis crops with haloduric performance are obtained and adaptability of the crops can be improved, and accordingly has important application prospect in breeding of the crops.

This invention is fake unicell bacteria Na+ / H+ antiport albumen gene and its clone method, it relates to one kind of gene clone. One kind of fake unicell bacteria Na+ / H+ antiport albumen structure gene and one kind of quickly and economic clone method are provided, gene length is 1089bp, coding 362 amino acids. Procedures are that extra anti-salt fake unicell bacteria is inoculated and cultivated, whole DNA is extracted, primer is designed, PCR reaction and amplified product is gel imaged system sweep record, then the amplified about 1.1kb tripe is cut down. One gland piao lin deoxyribonucleotide (A) is added to end of PCR product, above clear liquid is abandoned, and 20muL weight distilled water is added to dissolve DNA. Carrier is connected with DNA to form single colony. White colony is selected and recombination particle is extracted by caustic cracking solution. Goal gene is identificated and anti-salt level of transformant is detected, clone gene of transformant is sequenced and weight plasmid of transformant is identificated, then colone gene is estimated.

The invention discloses a recombinant bacterium producing γ-aminobutyric acid, its construction method and application. Adopt the method of the present invention to import glutamic acid decarboxylase B gene (gadB gene) into Escherichia coli mutant strain K12ΔgadABC through the expression vector pBAD / HisB. Glutamic acid: γ-aminobutyric acid antiporter is sensitive to pH, which effectively reduces the degradation of γ-aminobutyric acid and improves the yield and conversion efficiency of GABA. The transformation rate of KG01 strain was not affected by pH change, and the production of GABA was the highest, which was significantly higher than that of K12ΔgabT strain and K12 strain. Using glutamic acid with a concentration of 2M as the substrate, the KG01 strain was continuously transformed three times, and the transformation rate was above 99% each time, the yield was greater than 204g / L, and the substrate residue was low, which was convenient for downstream crystallization and purification. The gamma-aminobutyric acid produced by the genetically engineered bacteria constructed by the method of the invention has the advantages of low raw material, simple process, high production efficiency, etc., and has good industrial application prospect.

The invention provides cDNA of a switchgrass tonoplast Na<+> / H<+> antiporter protein gene PvNHX1, and is characterized in that the cDNA has a nucleotide sequence shown in SEQ ID No.1. The invention also provides a cloning method of the cDNA of the switchgrass tonoplast Na<+> / H<+> antiporter protein gene PvNHX1 according to the claim 1, wherein the method comprises the specific steps: (1) extracting total RNA of switchgrass, and carrying out reverse transcription into cDNA; (2) taking the cDNA as a template, and carrying out a high-fidelity full-length PCR reaction by a PvNHX1 gene full-length specific primer; and (3) recovering and purifying the PCR product of the step (2), carrying out an A addition reaction, and thus obtaining the cDNA of the switchgrass tonoplast Na<+> / H<+> antiporter protein gene PvNHX1. The method for obtaining the switchgrass PvNHX1 gene full-length cDNA sequence lays a foundation for vector construction, genetic transformation and the like by utilizing the sequence later on.

The present invention discloses a Na / H antiporter gene ZjNHX1 coming from zoysia japonica, an encoding protein and an application thereof. The gene is one of the following nucleotide sequences: (1) SEQ ID No:1 in the sequence table; (2) SEQ ID No:2 protein in the coding sequence table, and a nucleotide sequence 70 percent homologous to the 409th position to 2031th position of SEQ ID No:1 of the sequence table; (3) a nucleotide sequence capable of being hybridized with the 409th position to 2031th position of SEQ ID No:1 sequence in the sequence table under the medium stringency condition. The result of a functional complementation experiment conducted between the ZjNHX1 and the single and the double mutant of salt-sensitive yeast shows that the gene can reinstate the anti-salt capability of the salt-sensitive yeast to a certain degree. The present invention is applicable to the cultivation of novel salt-tolerant plant varieties or other novel genetically modified plant varieties with improved biological characters.

A Na<+> / H+ antiporter gene on a wheat plasma membrane and applications thereof are disclosed. The full length of the antiporter gene is 3460 bp. The gene after expression is positioned on a cell plasma membrane, and exerts a Na<+> / H+ exchange function to discharge Na<+> out of cells. A method of constructing a plant genetic transformation vector by utilizing the antiporter gene and improving plant salt tolerance is also disclosed.

The invention discloses a tamarix chinensis plasma membrane Na+ / H+ antiporter gene and an application thereof, and belongs to the technical field of gene engineering. The nucleotide sequence of the tamarix chinensis plasma membrane Na+ / H+ antiporter gene is shown as SEQ ID No. 1, and the amino acid sequence of the expressed protein is shown as SEQ ID No. 2. According to the invention, the tamarixchinensis plasma membrane Na+ / H+ antiporter gene is cloned and obtained from the tamarisk for the first time; arabidopsis thaliana, soybean, cotton and yeast which overexpress the tamarix chinensis plasma membrane Na+ / H+ antiporter gene are obtained by transforming the arabidopsis thaliana, the soybean, the cotton and the yeast; the salt tolerance is higher than that of the plasma membrane Na+ / H+antiporter gene of the untransformed or transformed soybean, cotton or arabidopsis thaliana; the result shows that the tamarix chinensis plasma membrane Na+ / H+ antiporter gene has an outstanding effect in the application in improving the salt tolerance of the plant or the yeast and has important application value in the breeding field of improving salt tolerance of plants or bacteria.

The present invention discloses an E. coli engineering bacteria producing 1,5pentanediamine through a whole cell catalysis and its application. The engineering bacteria according to the present invention, is Escherichia coli (E. coli) strain B or its derivative strains with the overexpression of a lysine decarboxylase gene and a proper expression of a lysinecadaverine antiporter gene cadB. The engineering bacteria according to the present invention is the engineering bacteria producing 1,5pentanediamine through the whole cell catalysis constructed from Escherichia coli B derivative strains, which has an overexpression of a lysine decarboxylase gene cadA and a proper expression of the lysinecadaverine antiporter gene cadB; The present invention further discloses a method of producing a 1,5pentanediamine catalyzed by the engineering bacteria, the yield and production intensity of 1,5pentanediamine in biobased production could be significantly improved through the method, hence it could be applied to mass production and convenient for extending applications.

The application discloses a kind of nano-assemblies of enhanced photodynamic-immunotherapy and its preparation method and application, belong to the technical field of pharmaceutical preparation.The nano-assemblies are co-assembled by photodynamic photosensitizer and cystine / glutamate antiporter inhibitor through intermolecular force of π-π stacking and hydrophobic interaction, and are surface-modified with amphiphilic PEG modifier, the molar ratio of the photodynamic photosensitizer and cystine / glutamate antiporter inhibitor is 10:1~1:10, and the mass ratio of the total mass of the photodynamic photosensitizer and cystine / glutamate antiporter inhibitor and the mass of PEG modifier is 60:40~95:5.The preparation method of the nano-assemblies of the application is simple and convenient, the drug loading efficiency is high, the efficient co-loading of drug can be realized, and the effect of enhancing photodynamic-immunotherapy is achieved, so the nano-assemblies have good clinical application prospect.

The invention provides a novel plant strong salt tolerant gene AtNHXS1 acquired through DNA reshuffling technology, and the ionic transport activity of Na+ / H+ antiporter protein coded by the gene is stronger than that of wild Na+ / H+ antiporter protein ATNHX1. A nucleotide sequence of the gene as shown in SEQ ID NO:1 also comprises genes of the nucleotide sequence of sequence 1 in a sequence tablewith the homology between 70 and 100 percent, or a nucleotide sequence of an amino acid sequence of sequence 2 in a coded sequence table. The new Na+ / H+ antiporter has a protein of the amino acid sequence of sequence 2 in the sequence table, protein of the amino acid sequence of the sequence 2 with the homology between 70 and 100 percent, or protein which replaces, deletes or adds one or a plurality of amino acids in the amino acid sequence of the sequence 2 with the same homology. The invention also provides the construction of recombinant vector, the transgenic plant and other methods so asto apply the gene and the protein, thereby cultivating a novel variety of transgenic plant with strong capacity of salt tolerance or other improved biologic characters.

The invention discloses a kind of Na on the wheat plasma membrane + / H + Antiporter genes and their applications. The Na + / H + The full length of the antiporter gene is 3460bp, and after expression, the gene is located on the plasma membrane of the cell and plays a role in Na + / H + exchange function, the Na + out of the cell, and thus the present invention also discloses the use of the Na + / H + The antiporter gene constructs a plant genetic transformation vector and a method for improving the salt tolerance of the plant.

The invention discloses a Trifolium repens.L Na<+> / H<+> antiporter protein gene TrNHX1 coming from the Trifolium repens.L, and the coded protein and the application thereof. The gene is one of the following nucleotide sequences: (1) SEQ ID No: 1 in a sequence table; (2) the polynucleotide of an SEQ ID No: 2 protein sequence in a coded sequence table; (3) a DNA sequence of functional protein with the same code, which has the homology by at least 70 percent with the limited DNA sequence of the SEQ ID No: 1 in the sequence table. The results of the functional complementation assay of TrNHX1 with salt-sensitive single and double mutants of yeast prove that the gene can recover the salt resistance to a certain extent. The gene of the invention can be applied to the transformation of the Trifolium repens.L, leguminous plants and other plants as well as the culture of new salt-resistant varieties of the plants or other new varieties of transgenic plants, the biological characters of which are improved.

The invention discloses a tamarisk plasma membrane Na + / H + The antiporter gene and its application belong to the technical field of genetic engineering. The Tamarix plasma membrane Na + / H + The nucleotide sequence of the antiporter gene is shown in SEQ ID No.1, and the amino acid sequence of the expressed protein is shown in SEQ ID No.2. The present invention clones Tamarix plasma membrane Na for the first time from Tamarix + / H + antiporter gene, through transformation of Arabidopsis, soybean, cotton and yeast, overexpressed Tamarix plasma membrane Na + / H + Arabidopsis thaliana, soybean, cotton and yeast with antiporter gene have lower salt tolerance than untransformed or transformed soybean, cotton or Arabidopsis plasma membrane Na + / H + antiporter gene was higher, indicating that Tamarix plasma membrane Na + / H + The antiporter gene has an outstanding effect in the application of improving the salt tolerance of plants or yeast, and has important application value in the field of improving the salt tolerance breeding of plants or strains.

The invention discloses a karelinia caspica(Pall.)Less. plasma membrane Na+ / H+ reversal transport protein and an encoding gent and application thereof. An amino acid residue sequence of the kareliniacaspica(Pall.)Less. plasma membrane Na+ / H+ reversal transport protein KcSOS1 is shown in SEQ ID No.2 in a sequence table; a sequence of the gene encoding the karelinia caspica(Pall.)Less. plasma membrane Na+ / H+ reversal transport protein is shown in SEQ ID No.1. It is proved through an experiment that when a karelinia caspica(Pall.)Less. KcSOS1 gene is transferred into tobacco, the saltresistanceof the transgenic tobacco can be significantly improved. The provided karelinia caspica(Pall.)Less. KcSOS1 gene can be used for improving the salt stress resistance performance of crops, and has a wide application prospect in the field of salt tolerance inheritance improvement for the crops and fine forage grass.

Imaging agents that comprise labeled substrates of the cystine / glutamate antiporter of cells, whereby the methods of use comprise introducing the labeled agents into cells via the cystine / glutamate antiporter, which are then reduced to a labeled cysteine, and subsequently detected in the cell.

The invention discloses a thinopyrumponticum tonoplast Na+ / H+ reverse transport protein 2 gene, and the application of the gene sequence in improving crop and forage grass' salt resistant character by means of biological technique. The provided gene is a member of the Na+ / H+ antiporter family on the Thinopyrumponticum vacuole membrane, the gene shows positive relativity to the salt tolerance of the Thinopyrumponticum. The disclosed gene sequence can be employed to clone the gene and construct pronucleus expression carrier. The mass expression in procaryotic cells can produce Na+ / H+ antiporter 2 for protein structural research, functional research and antigen preparation. After cloning the gene based on the gene sequence, various pronucleus expression carriers can be constructed, by leading the gene into crops and forage grass, strains / lines with high salt tolerance property can be cultivated.

The invention belongs to the technical field of molecular biology, and particularly relates to a genetically engineered bacterium for synthesizing 1, 5-pentanediamine and a method for synthesizing the 1, 5-pentanediamine. According to the invention, lysine decarboxylase and lysine-cadaverine antiporter are co-expressed in a basic strain of high-yield L-lysine, the obtained genetically engineered bacterium synthesizes L-lysine by using a carbon source, the L-lysine is converted into 1, 5-pentamethylene diamine under the action of the lysine decarboxylase, and the synthesized 1, 5-pentamethylene diamine is converted into 1, 5-pentamethylene diamine through the lysine-cadaverine antiporter. According to the present invention, the 1, 5-pentanediamine is effectively transferred to the outside of the cell, such that the one-step conversion from the carbon source, especially the glucose, to the 1, 5-pentanediamine is achieved, the efficient synthesis of the 1, 5-pentanediamine is achieved, the process is simple, and the production cost is low. Results of embodiments show that the concentration of 1, 5-pentanediamine synthesized by using the genetically engineered bacterium provided by the invention is 3.35-4.90 mM, and the conversion rate of glucose is close to 100%.

The invention belongs to the technical field of microbial engineering and genetic engineering, and discloses escherichia coli for producing L-histidine as well as a construction method and application of the escherichia coli. The escherichia coli contains a growth dependent type promoter PprpB of a methyl isocitrate lyase gene prpB and / or a stable phase dependent type promoter PydgI of an ornithine reverse transport protein / lysine permease gene ydgI. According to the escherichia coli for producing L-histidine, expression of glutamine phosphoribose pyrophosphate transamidase gene purF is regulated and controlled by using growth dependent promoter PprpB of methylisocitrate lyase gene prpB, and table of ATP phosphoribose transferase gene hisG is regulated and controlled by using stable phase dependent promoter PydgI of ornithine reverse transport protein / lysine permease gene ydgI According to the invention, cell growth energy supply in the earlier stage of thalli and L-histidine metabolic flux in the later stage of thalli production can be realized, so that balanced distribution of metabolic flux between cell growth of a microbial cell factory and L-histidine production is effectively balanced.

Disclosed are a tonoplast sodium-hydrogen anti-protein NHX2 derived from Thellungiella halophila and coding gene thereof, and use thereof in the cultivation of transgenic plants having improved salt tolerance.

The invention relates to tonoplast proton / sugar antiporter proteins, more particularly tonoplast proton / saccharose antiporter proteins, the nucleotide sequences which encode them and uses thereof for producing transgenic plants with an increased saccharose concentration. The invention also includes methods for producing transgenic plants with an increased saccharose concentration, methods for increasing the saccharose concentration in plants, and for identifying plants which are suitable for creating a higher saccharose concentration.