34 results about "Zoysia" patented technology

The invention relates to a primer and method for recognizing zoysia japonica varieties. The nucleotide sequences of the primer are shown as the SEQ ID No.1 and the SEQ ID No.2. The invention further provides a method for recognizing the zoysia japonica varieties. The method comprises the steps that a genome DNA of a sample to be tested is used as a template DNA, and PCR amplification is carried out by means of the primer. Compared with an existing three-nature testing and recognizing method (namely the appearance, morphological characteristic description method) for recognizing zoysia japonica varieties, testing results are not affected by environment factors, operation is convenient and rapid, the testing period is obviously shortened, and the testing results are more accurate and reliable.

The invention belongs to the field of molecular biology, and discloses a salt-tolerant gene ZmPDI in a halophyte zoysia matrella as well as a plant expression vector and application thereof. The sequence of the salt-tolerant gene ZmPDI in the zoysia matrella is SEQ ID NO. 1. The plant expression vector is obtained by carrying out double enzyme digestion on ZmPDI through BamHI and EcoRV, then inserting the ZmPDI into a gateway entry vector pENTR1A, and finally subjecting the ZmPDI and a pEarleyGate103 expression vector plasmid to recombination reaction. The ZmPDI in the zoysia matrella, which is provided by the invention, is a novel salt-tolerant gene; the gene can be used for improving the salt tolerance of a plant, and can be applied to the creation of novel salt-tolerant germplasm and the improvement of a plant variety.

The present invention provides a gene gun-mediated high-efficiency gene editing method for Zoysia japonica, designing an sgRNA sequence based on CRISPR / Cas9 for the target gene ZjSGR in Zoysia japonica, and connecting the DNA fragment containing the coding sgRNA sequence to a gene carrying CRISPR In the vector of CRISPR / Cas9, Zoysia japonica is transformed by the gene gun-mediated transformation method, or the vector containing the DNA fragment encoding the sgRNA sequence, the vector carrying CRISPR / Cas9 and the vector containing the selection marker gene are transformed by the gene gun-mediated transformation method. The transgenic Zoysia japonica plants were co-transformed with the inductive transformation method to realize the site-directed mutation of the Zoysia japonica gene ZjSGR, and then to obtain the transgenic Zoysia japonica plants lacking the function of the gene. The invention provides a gene gun-mediated high-efficiency gene editing method of Zoysia japonica, as a tool for directional editing of Zoysia japonica genes, and the editing efficiency is as high as 37.5%.

The invention discloses a strain of Helminthrospora jipingi, its screening method and its application. Umbilicale sp. Jiping strain (SX-5-2) has been preserved in the General Microorganism Center of China Committee for the Collection of Microorganisms, and the preservation number is CGMCC NO.13887. The concentration of conidia utilizing this strain was 10 per milliliter 4 More than one is used for biological weeding, and can be safely used in rice, wheat, sorghum, soybean, cotton, vegetable crop fields and tall fescue and zoysia grass lawns to control crabgrass, goosegrass, foxtail, Grass weeds such as barnyardgrass, stephenia chinensis, weetweed, bluegrass, and bluegrass, as well as weeds of the genus Miscanthus, Miscanthus, goldengrass, Japanese weed, weed, and sudan grass. The control effect on target weeds can reach more than 80%. It has the characteristics of safety and environmental protection.