34 results about "Zoysia" patented technology

The invention relates to a primer and method for recognizing zoysia japonica varieties. The nucleotide sequences of the primer are shown as the SEQ ID No.1 and the SEQ ID No.2. The invention further provides a method for recognizing the zoysia japonica varieties. The method comprises the steps that a genome DNA of a sample to be tested is used as a template DNA, and PCR amplification is carried out by means of the primer. Compared with an existing three-nature testing and recognizing method (namely the appearance, morphological characteristic description method) for recognizing zoysia japonica varieties, testing results are not affected by environment factors, operation is convenient and rapid, the testing period is obviously shortened, and the testing results are more accurate and reliable.

The invention relates to a method for molecularly identifying the genetic markers and the variety of a plant, and specifically discloses a group of SRAP (Sequence-Related Amplified Polymorphism) marking primers and an identification method for identifying Suzhi No.1 hybrid zoysia. The group of primers is used for PCR (Polymerase Chain Reaction) to obtain the amplified fragments of each pair of primers; the obtained fragments are compared with genome fragments disclosed by the invention; consequently, whether the zoysia variety is the Suzhi No.1 hybrid zoysia can be identified accurately.

The invention belongs to the field of molecular biology, and discloses a salt-tolerant gene ZmPDI in a halophyte zoysia matrella as well as a plant expression vector and application thereof. The sequence of the salt-tolerant gene ZmPDI in the zoysia matrella is SEQ ID NO. 1. The plant expression vector is obtained by carrying out double enzyme digestion on ZmPDI through BamHI and EcoRV, then inserting the ZmPDI into a gateway entry vector pENTR1A, and finally subjecting the ZmPDI and a pEarleyGate103 expression vector plasmid to recombination reaction. The ZmPDI in the zoysia matrella, which is provided by the invention, is a novel salt-tolerant gene; the gene can be used for improving the salt tolerance of a plant, and can be applied to the creation of novel salt-tolerant germplasm and the improvement of a plant variety.

The invention discloses a weed-resistant zoysia seed pellet coating material and a coating method thereof, which belong to the technical field of seed processing. The coating material of the present invention includes a binder, an inert substance and an active substance, and the active substance is one or more of sulfasulfuron-methyl, quinclorac, xiubaigong or zalufeng. The germination rate of the pelletized formula provided by the invention is the same as that of the naked seed, and the growth vigor is better than that of the naked seed. The obtained zoysia seeds have weed resistance, and can efficiently save manpower and material resources in terms of weed control costs.

The invention relates to clone and functional identification of a stress-induced promoter Rd29A in zoysia. The invention comprises the following main steps: the zoysia is taken as a plant material, and total DNA of the plant is extracted; a pair of special primers is designed by biological software, and the sequence of the Rd29A promoter is obtained by the PCR amplification method; two plant eukaryon expression vectors, namely pBIG(35S-GFP) and pBIRG(Rd29A-GFP) are respectively established on the basis of pBI121; epidermic cells of an onion are transformed by the particle gun method; and the activity of the Rd29A promoter in adjusting and controlling GFP gene expression in receptor cells is detected. The invention can screen out the stress-induced promoter Rd29A which is suitable for genetransfer of turfgrass; and the Rd29A promoter is a special promoter which is induced to express by adverse circumstances such as drought, saline and alkaline, low-temperature and so on, is also an ideal induced promoter in the plant resistance genetic engineering, and has important significance on the growth and development of the turfgrass under the condition of adverse circumstances.

The invention relates to a method for identifying authenticity of filial generation of zoysia and manilagrass, which belongs to the field of biological technology. The invention is specially for rapid and precise identification of authenticity of filial generation of Qingdao zoysia and manilagrass. Using a genome DNA template of nine zoysia filial generations and two parents Qingdao zoysia and manilagrass as the template, select three pairs of SRAP primers, namely primers capable of amplifying specific band not included in female parent on male parent. Perform amplifying and electrophoresis on the male parent, the female parent and the filial generation, and identify according as whether the F1 generation can amplify male parent specific band. The result shows that a filial generation is a real hybrid when each of the nine filial generations has male parent specific band. Thus, a molecular identification system of authenticity of filial generation of Qingdao zoysia and manilagrass is established, by which the authenticity of filial generation of Qingdao zoysia and manilagrass can be quickly identified, the method can also be used for hybrid variety fingerprint map construction andpurity identification.

The invention provides a cultivation method of mixed-lawn, providing the cultivation method of novel mixed-lawn, which solves the problems of high maintenance cost and high consumption of the water, nutrient and energy for the cold season lawn, as well as the problems of short green stage, poor greening effect and difficult cultivation for the warm season lawn through the mixed-lawn method of thestalked meadow grass which is the cold season lawn grass and the zoysia which is the warm season lawn grass, and the process of preparing, snowing, maintaining the seedling, pruning, managing the finished lawn and the like.

The invention belongs to the field of molecular biology and discloses a salt-resistant gene ZmGnTL of a halophyte zoysia matrella and an expression vector and an application thereof. The sequence of a novel salt-resistant gene ZmGnTl in zoysia matrella is SEQ ID NO.1. The plant expression vector is obtained by performing a recombination reaction with pEarleyGate103 expression vector plasmids after inserting ZmGnTL after double enzyme digestion of BamHI and NotI into a gateway entry vector pENTR1A. The ZmGnTL of zoysia matrella provided by the invention is the novel salt-resistant gene which can improve the salt resistance of the plant and can be applied to creating salt-resistant novel germplasms and improving plant varieties.

The present invention provides a gene gun-mediated high-efficiency gene editing method for Zoysia japonica, designing an sgRNA sequence based on CRISPR / Cas9 for the target gene ZjSGR in Zoysia japonica, and connecting the DNA fragment containing the coding sgRNA sequence to a gene carrying CRISPR In the vector of CRISPR / Cas9, Zoysia japonica is transformed by the gene gun-mediated transformation method, or the vector containing the DNA fragment encoding the sgRNA sequence, the vector carrying CRISPR / Cas9 and the vector containing the selection marker gene are transformed by the gene gun-mediated transformation method. The transgenic Zoysia japonica plants were co-transformed with the inductive transformation method to realize the site-directed mutation of the Zoysia japonica gene ZjSGR, and then to obtain the transgenic Zoysia japonica plants lacking the function of the gene. The invention provides a gene gun-mediated high-efficiency gene editing method of Zoysia japonica, as a tool for directional editing of Zoysia japonica genes, and the editing efficiency is as high as 37.5%.

The invention relates to a rapid propagation method for zoysia. The rapid propagation method comprises the following steps: sowing zoysia seeds on a matrix, and spraying water; enabling a surface covering to be covered on the surface of the seeds where water is sprayed; respectively watering the seeds once when illumination and darkness are alternated with each other each day at a germination period of the seeds; respectively watering the seeds once when illumination and darkness are alternated with each other each day at a seedling stage after the seeds germinate; completely removing the surface covering when each seedling has two leaves and is 4-5 cm tall; and performing watering maintenance until the seedlings grow up. Any exogenous chemical is not used for treatment, the seeds do not need treating alone, and the method is simple, fast and easy to operate.

The invention discloses a method for improving the winter resistance of Japanese lawngrass. This method includes converting transcription activation factor CBF1 / DREB1b of quasi-mustard induction gene into Japanese lawngrass embryonal callus to improve the winter resistance of Japanese lawngrass, which can include the following steps: 1) disseminating the Japanese lawngrass embryonal callus with agricillin liquor carrying CBF1 / DREB1b; 2) placing the disseminated Japanese lawngrass embryonal callus onto the coculture medium to perform coculture; 3) placing the cocultured embryonal callus onto the screening culture medium for screening; 4) placing the screened embryonal callus onto the differentiated culture medium for differentiation culture; 5) placing the differentiated and germinated young plant onto the rooted culture medium for radication; 6) placing the plant which can produce root onto strong-plant screening culture medium for screening the Japanese lawngrass strong-plant which has improved winter resistance. The invention has important theoretical and operation significance for culturing new species of Japanese lawngrass with improved cold resistance and extended green period and has a wide application foreground.

The invention discloses a pre-emergent herbicide for lawn weeds, which comprises organic heterocycles, triazolopyrimidine sulfonamides and substituted pyridyl anilides. In the invention, organic heterocycles, triazolopyrimidine sulfonamides and substituted pyridyl anilides are compounded, and the three have a synergistic effect on each other, which can obviously improve the pre-emergence weeding rate of lawn weeds. The herbicide of the invention has a long duration of action and a wide application range, and is suitable for lawns such as bermudagrass, zoysia, sea paspalum and the like.

The invention provides a method for establishing a lawn mixed with zoysia and white clover. The technical scheme adopted is: germination in warm water, seed soaking in medicinal solution, scientific mixed sowing ratio, dry and wet seed sowing amount conversion method, layered sowing and layered covering method, and control of fertilization and watering amounts, plus the use of agricultural plastic film to punch holes The application of technologies such as controlling temperature, humidity, and light ensures the various conditions required for the simultaneous germination of mixed turfgrass seeds, the growth of seedlings, and the formation of lawns. After the steps of seed treatment, mulch perforation, site preparation before turf construction, sowing, control of temperature and humidity in the germination period, and maintenance of seedlings in the film, it provides a method that can solve the problem of high maintenance costs in cold seasons, easy degradation of lawns, and A new hybrid lawn planting method that solves the problem of short green period in warm season and affects the greening effect and is difficult to popularize.

The invention discloses a strain of Helminthrospora jipingi, its screening method and its application. Umbilicale sp. Jiping strain (SX-5-2) has been preserved in the General Microorganism Center of China Committee for the Collection of Microorganisms, and the preservation number is CGMCC NO.13887. The concentration of conidia utilizing this strain was 10 per milliliter 4 More than one is used for biological weeding, and can be safely used in rice, wheat, sorghum, soybean, cotton, vegetable crop fields and tall fescue and zoysia grass lawns to control crabgrass, goosegrass, foxtail, Grass weeds such as barnyardgrass, stephenia chinensis, weetweed, bluegrass, and bluegrass, as well as weeds of the genus Miscanthus, Miscanthus, goldengrass, Japanese weed, weed, and sudan grass. The control effect on target weeds can reach more than 80%. It has the characteristics of safety and environmental protection.