The invention discloses a hundred-herb distiller yeast and a preparation method thereof. The hundred-herb distiller yeast comprises the following one hundred raw materials in weight ratio: paris polyphylla smith, trifoliate acanthopanax root, obscured homalomena rhizome, eleutherine plicata herb, embelia subcoriacea, nidus vespae, purslane, kapok, rubia yunnanensis, nightshade, dragon tree, alpinia conchigera, safflower, melothria heterophylla, zanthoxylum planispinum, gragrant solomonseal rhizome, aloe, houttuynia cordata, rhizoma cibotii, cyperus rotundus, lemon grass, cinnamomum hupehanum, sagina subulata, etlingera yunnanense, tinospora sinensis merr, asarum, herba solani nigri, scopia dulcis, zanthoxylum simulans, purple perilla, dipterocarpus turbinatus, sandalwood, eclipta alba and the like; and the hundred-herb distiller yeast is prepared according to a certain method. After being taken moderately, pure grain spirit brewed by using the hundred-herb distiller yeast has the functions of building body, resisting aging, nourishing skin, facilitating uresis and expelling stone, activating blood circulation to dissipate blood stasis, relieving cough and asthma, circulating qi and blood, dispelling wind and relieving pain, killing parasites to relieve itching, refreshing and restoring consciousness, detoxifying and invigorating stomach, calming, adjusting and replenishing water and blood, connecting muscle and bones, nourishing yin and supplementing kidney and the like.