4 results about "Cantharides" patented technology

The application discloses a biological additive for promoting poultry feather development and application thereof, and belongs to the technical field of animal breeding. The biological additive is prepared from scallop skirt oligopeptide and cantharides polypeptide at a mass ratio of 1:1. Animal experiments show that, when meat chickens are fed with 60 mg of the biological additive per 1 kg of basic feed, the growth of the meat chickens can be promoted, and more importantly, the feather development of the meat chickens can be significantly and synergistically promoted, and the length of the main wing feathers is obviously increased. Cytological studies further prove that the biological additive can synergistically promote the proliferation of poultry hair follicle epidermal stem cells, and the effect is better than that of an EGF positive control. Therefore, the biological additive developed by the application is safe, efficient and green, effectively solves the problems of drug residues and drug resistance possibly caused by traditional chemical additives, provides a sustainable solution for poultry breeding, and has important economic and application values.

According to the external traditional Chinese medicine composition liniment for treating alopecia areata and the application thereof, eight medicinal materials including eclipta alba, cacumen biotae and the like which have the effects of warming and promoting blood circulation, dispelling wind and removing fat are matched according to a specific proportion, and active ingredients are reserved by adopting a step-by-step soaking process. The huechys sanguinea, the cantharides and the seven plant medicines are compounded for the first time; a special soaking process of sub-packaging first and then combined extraction is established; key parameters such as ethanol concentration and soaking time are determined; the treatment effect on five types of alopecia is verified. Clinical pre-tests show that the hair regeneration rate of an alopecia areata patient can reach 78.6% after the traditional Chinese medicine is continuously used for 4 weeks.

The invention discloses a method for simultaneously detecting the content of two polypeptides in cantharides, which is characterized in that characteristic polypeptides BM-1 (AGDDAPRAVF) and BM-2 (YSF) are taken as indexes, and a high performance liquid chromatography-tandem mass spectrometry technology is adopted for quantitative analysis. The method comprises the steps of sample enzymolysis, chromatographic separation and mass spectrometric detection, and has the characteristics of strong specificity, high sensitivity and good accuracy. Meanwhile, the invention provides a matched detection kit which comprises key components such as a reference substance, an enzymolysis reagent and a chromatographic mobile phase. The method can be used for quality evaluation of cantharides medicinal materials, quality control of decoction pieces and authenticity identification, and a new technical means is provided for perfecting a cantharides quality evaluation system.