26 results about "Bufotoxin" patented technology

This invention belongs to the field of traditional Chinese medicine, specifically relating to a topical delay spray and its preparation method. The invention uses the dried epidermis of the Huaxi toad (with the toad venom removed), and extracts it efficiently through a combination of water and alcohol extraction. Male silkworm moth oil can nourish and strengthen the body, tonify the liver and kidneys, and combat fatigue; studies have shown that male silkworm moth oil can effectively treat kidney yang deficiency. This invention combines the synergistic effects of toad skin extract and male silkworm moth oil to prepare a delay spray that can significantly treat premature ejaculation and erectile dysfunction in men. This preparation has the advantages of direct action, sustained release, avoidance of drug contact, and simple operation. This invention further improves the rational utilization system of toad resources, provides a practical solution for the efficient development of toad medicinal resources, and significantly reduces production and clinical application costs.

The application belongs to the field of traditional Chinese medicines, and particularly relates to a more low-irritation analgesic ancient prescription microemulsion gel and application thereof in anti-arthritis drugs. The application finds that after water-soluble and fat-soluble components of black plaster prepared from raw frankincense, myrrh, eaglewood, dragon's blood, Strychnos nux-vomica, Panax notoginseng, Oldenlandia diffusa, Venenum Bufonis, bovine bezoar, Stachys jamaicensis, Platycodon grandiflorum, Hirudo nipponia, Tinea, Solenopsis geminata, Scorpio, scorpio, red lead and sesame oil are extracted, a more low-irritation analgesic ancient prescription microemulsion gel is prepared; compared with the black plaster itself, the analgesic effect of the microemulsion gel is significantly improved, and the microemulsion gel has good anti-inflammatory effect and can be used for preparing anti-arthritis drugs; and the microemulsion gel has reduced toxicity and is more convenient to use.

This invention relates to an oral nanomedicine antigen delivery system and its construction method and application, belonging to the field of oral nanomaterials technology; the construction method includes the following steps: (1) preparation of OM nanoparticle suspension; (2) preparation of cationic liposome suspension; (3) preparation of cationic liposome suspension loaded with OM / BF; (4) construction of oral nanomedicine antigen delivery system. This invention breaks through multiple barriers in the gastrointestinal tract, improves drug bioavailability, and achieves precise immune tracking through the OVA tumor antigen model, simulating in vivo anti-tumor CTL response, thereby exerting anti-tumor effects synergistically at the cellular, tissue, and immune levels, effectively solving the problems of poor water solubility and low delivery efficiency of the active ingredient bufotoxin in traditional Chinese medicine. At the same time, through macrophage-mediated immune regulation and precise drug release, it improves targeting and treatment efficiency, opening up a new avenue for tumor immunotherapy with oral nanomedicine.

The invention relates to the technical field of venenum bufonis processing, in particular to venenum bufonis processing equipment and method.The venenum bufonis processing equipment comprises a base, a mechanical arm, a discharge promoting module, a flexible attaching module, an adsorption recycling module and a main controller. And the drainage promoting module is used for performing non-destructive stimulation on the posterior auricular gland to induce drainage. The flexible attaching module is provided with a flexible scraping head which can deform in a self-adaptive mode to be attached to the surface of a gland, and a flexible touch sensor array for sensing pressure distribution and a micro pneumatic cavity grid array for driving deformation are arranged in the flexible attaching module. The adsorption recovery module is integrated on the working face of the scraping head and is provided with a micro-suction hole network capable of independently controlling adsorption force in different areas. And the main controller coordinates all the modules to work in sequence: firstly promoting discharge, then controlling the flexible scraping head to perform scraping in a fitting state, meanwhile, intelligently adjusting adsorption force of different areas according to real-time pressure distribution so as to recover slurry, and constraining global contact force through a multi-dimensional force sensor so as to ensure safety.

The invention provides a traditional Chinese medicine compound external preparation for invigorating the kidney and strengthening Yang and a preparation method thereof, belongs to the technical field of traditional Chinese medicine preparations, and aims to solve the problems that the bioavailability of the first-pass effect of existing oral administration is low, and effective components of external ointment are difficult to penetrate through the skin. The preparation is a composite gel containing a penetration enhancer and a temperature-sensitive gel matrix, and the traditional Chinese medicine compound at least comprises various active ingredients of male silkworm moth, centipede, epimedium, rhizoma curculiginis, cistanche, schisandra chinensis, semen cuscutae, polygala tenuifolia, fructus cnidii, semen allii tuberosi, actinolite, sulfur, venenum bufonis, amethyst and borneol. According to the preparation disclosed by the invention, the transdermal permeation rate and the cumulative permeation amount of various active ingredients in the compound are improved in order of magnitude, so that an effective treatment concentration can be ensured to be reached in an in-vitro local or in-vivo system. In clinical application, the traditional Chinese medicine composition has better effect taking speed and treatment effect than external application or oral administration of traditional prescriptions with the same name.

The application belongs to the field of biological pharmacy, and particularly discloses a preparation method of musk heart-activating dropping pills, which comprises the following steps: preparing a salvia miltiorrhiza clear paste, mixing the salvia miltiorrhiza clear paste, artificial musk, total ginsenosides of ginseng stems and leaves, toad venom, artificial ox gall, bear bile powder and borneol with polyethylene glycol 6000 and polysorbate 80, stirring and uniformly mixing, dropping, centrifuging, screening, drying and coating to prepare the musk heart-activating dropping pills. The application has remarkable clinical curative effect, no side effect, high drug stability, fast dissolution, high efficiency and rapid effect. The application further discloses a preparation device of the musk heart-activating dropping pills, wherein the vertical silica gel cleaning strips, the first silica gel cleaning strips and the second silica gel cleaning strips in the silica gel cleaning net frame and the silica gel cleaning strip component are cross arranged, can be in friction contact with different surfaces of the salvia miltiorrhiza in the cleaning process, and can provide comprehensive and detailed cleaning effect. The design not only increases the cleaning area, but also can effectively remove the small dust and impurities on the surface of the salvia miltiorrhiza, and avoids the dead angle which is easily missed in the traditional cleaning mode.

The invention discloses eye protection liquid and a preparation method thereof, and belongs to the technical field of traditional Chinese medicine external preparations. The eye protection liquid is prepared from a chrysanthemum extract, borneol, calculus bovis factitius, venenum bufonis, realgar, a mint extract, a pipewort extract, a feather cockscomb seed extract, a butterflybush flower extract, pearl powder and purified water according to a specific weight part ratio, and is prepared by soaking, distilling, filtering and filling. The traditional Chinese medicine composition can rapidly permeate into eyes and directly enter focus, can relieve and treat various eye diseases such as presbyopia, myopia, amblyopia, cataract and the like, has the effects of soothing liver and relieving depression, promoting blood circulation and dredging collaterals, tonifying kidney and nourishing yin, supplementing optic nerve nutrition and improving microcirculation around the eyes, has the total effective rate of 100% and the cure rate of 80-95%, and is free of side effects and drug resistance and convenient to use.

This invention discloses a composition and its preparation method for treating human papillomavirus (HPV) and cervical intraepithelial neoplasia (CIN), belonging to the field of traditional Chinese medicine technology. The formula consists of 6-15 parts of American cockroach, 5-11 parts of hawthorn, 5-11 parts of chicory, 3-9 parts of *Scutellaria baicalensis*, 8-20 parts of *Sophora flavescens*, and 6-15 parts of *Cnidium monnieri*, and also contains 0.01-1 parts by weight of toad venom. The dosage form is one or more of the following: external gel, rinse, foam, suppository, and film preparation. It has high safety and is suitable for HPV infection and CIN changes, solving the problem of poor efficacy in existing treatments.

The application belongs to the technical field of traditional Chinese medicines, and particularly relates to application of a traditional Chinese medicine composition in preparation of a liver cancer resisting medicine. The traditional Chinese medicine composition comprises red ginseng, ox horn, musk, borneol, safflower, ox gall, appendices, toad venom, panax notoginseng, storax and pearl. Experimental results show that the traditional Chinese medicine composition can induce apoptosis and necrosis of liver cancer cells, inhibit proliferation of the liver cancer cells, and block the liver cancer cells in the G1 phase of the cell cycle. Therefore, the application provides a new treatment medicine for clinical treatment of liver cancer, and the raw materials are natural medicinal materials, are widely sourced, and have a good application prospect.

This invention belongs to the field of biomedical technology, specifically relating to an ointment and its application. The toad oil used in this invention differs significantly in composition from toad venom, and its ointment is effective for various acute and chronic pain conditions. Animal experiments have confirmed that the toad oil ointment significantly reduces the frequency and delays the onset of acetic acid-induced writhing responses, demonstrating a clear analgesic effect. Clinical studies have shown that this ointment is rapidly effective and has a definite therapeutic effect on acute gout attacks, postherpetic neuralgia, and arthritis-related pain and swelling. The formulation effectively improves drug retention and user comfort, and no skin irritation has been observed. This development achieves comprehensive utilization of toad resources, providing an example for the sustainable development of traditional Chinese medicine and the efficient utilization of biological resources.

This invention discloses a library of traditional Chinese medicine (TCM) compositions based on general and local anesthesia mechanisms, along with various dosage forms and preparation methods. The invention constructs a systematic library of TCM anesthetic compositions: the general anesthetic composition consists of extracts from herbs such as Datura stramonium, Datura stramonium, processed Aconitum carmichaelii, processed Aconitum kusnezoffii, Saussurea involucrata, Jasminum sambac root, Rhododendron molle, and Sanqie Sanqie; the local anesthetic composition consists of extracts from herbs such as Asarum heterotropoides, Murraya paniculata, Ephedra sinica, Toad venom, and Corydalis yanhusuo. This invention further develops the above compositions into various modern dosage forms, including injections, tablets, capsules, and sprays. In particular, this invention innovatively introduces a clove-cinnamon natural preservative and flavoring system to replace chemical preservatives. In terms of preparation technology, advanced technologies such as supercritical CO2 extraction, microwave-assisted extraction, nano-embedding, and nanoemulsions are integrated, and a self-extraction scheme is adopted to achieve controllable quality and cost. Simultaneously, a quality detection method based on HPLC fingerprinting is established. This invention achieves full-scenario coverage of anesthesia and analgesia, from general to local, from emergency to routine care, with outstanding advantages of safety, effectiveness, and controllable quality.

The utility model discloses a multifunctional toad venom collector which comprises a support and a protective cover, the protective cover is fixedly connected with the support, the upper surface of the protective cover is fixedly connected with a mounting frame, one surface of the mounting frame is fixedly connected with a motor, the output end of the motor is fixedly connected with a screw rod, and the peripheral side face of the screw rod is provided with a moving block. The bottom face of the moving block is fixedly connected with an electric push rod, the output end of the electric push rod is fixedly connected with a connecting plate, a plurality of guide grooves are formed in the upper surface of the connecting plate, guide rods are installed in the guide grooves, springs are installed on the circumferential side faces of the guide rods, the bottom ends of the guide rods are fixedly connected with an extrusion soft plate, and a mesh plate is fixedly connected into the support. The toad collecting device solves the problems that in the prior art, toads are directly grabbed by hands and collected through a scraper, and the collecting method is prone to causing the fact that serous fluid is splashed into the eyes and harms the eyes.

The invention discloses a traditional Chinese medicine composition for treating wound ulcer and a preparation method thereof, and belongs to the technical field of traditional Chinese medicines. The prescription is composed of 5-15 parts of slender gouania root, 5-10 parts of maggot, 0.01-0.5 part of venenum bufonis, 5-10 parts of American cockroach, 5-15 parts of abelmoschus manihot, 3-9 parts of rheum officinale, 1-5 parts of safflower and 5-15 parts of fructus chebulae, the dosage form is external ointment, powder, oil, liquid preparation and the like, the traditional Chinese medicine composition is remarkable in curative effect, the cure rate can reach 85% or above after 2 months of treatment, the average healing time is shortened by 40 days or above, safety is high, and the traditional Chinese medicine composition is suitable for clinical application. The traditional Chinese medicine composition is suitable for chronic intractable wound ulcer such as diabetic foot ulcer, and the problem of poor treatment effect in the prior art is solved.

The application belongs to the field of traditional Chinese medicine, and particularly relates to an external use delayed oil agent and a preparation method thereof. The dried skin of the Visternotus limnocharis which has scraped toad venom is combined with the male silkworm moth which has the functions of tonifying liver and kidney, strengthening yang and astringing essence to significantly improve male premature ejaculation and erectile dysfunction through water extraction and alcohol extraction. The male silkworm moth oil has unique promotion potential and development potential. The application combines the toad skin and the male silkworm moth oil to prepare the delayed oil agent, significantly improves male premature ejaculation and erectile dysfunction, and has the characteristics of direct action and simple operation. The preparation cost is low.

The invention discloses a traditional Chinese medicine preparation for treating chronic esophagitis and esophageal precancerous lesions and a preparation method thereof. The traditional Chinese medicine preparation is prepared from nux vomica, sal ammoniac, sculellaria barbata, Chinese lobelia, duchesnea indica, marsdenia tenacissima, solanum septemlobum bunge, selfheal, processed pinellia ternate, ginger, coptis chinensis, sticktight, airpotato yam, radix clematidis, borax, platycodon grandiflorum, hawthorn, kelp, seaweed, edible tulip and venenum bufonis. The preparation method comprises the following steps: processing or performing raw treatment on the materials, grinding into fine powder by using a grinder, finely screening by using a sieve, removing coarse stems, and mixing in parts by weight to obtain a finished product of the preparation. The traditional Chinese medicine is simple in preparation method and low in cost, can dissipate blood stasis, regulate liver and stomach and nourish stomach yin, and can effectively treat chronic esophagitis and esophageal precancerous lesions.

The utility model discloses a venenum bufonis decompression drying room, and relates to the technical field of traditional Chinese medicinal material processing, and the venenum bufonis decompression drying room is technically characterized by comprising a room body, a plurality of placing plates are fixedly installed on the inner walls of the two sides of the room body, a rotating pipe is rotatably installed in the room body in a penetrating mode, and a plurality of connecting pipes communicated with the interior are fixedly installed on the outer wall of the rotating pipe; the ends of the two connecting pipes on the same side are jointly and fixedly connected with a drying pipe, the outer walls of the two drying pipes are each provided with a plurality of air outlet holes communicated with the interior, and a connecting sleeve is fixedly installed on the outer wall of the rotating pipe. The first motor can drive the rotating pipe to rotate through the two transmission wheels connected through the belt, and then the rotating pipe drives the two drying pipes to rotate through the connecting pipe, so that the rotating pipe can rotate while warm air is blown out, the warm air can be uniformly distributed in the room body, and the venenum bufonis placed in the room body can be more uniformly dried.

The invention provides application of a venenum bufonis extract combined with beta-lactam antibiotics in medicines for treating MRSA infection, and belongs to the technical field of biological medicines. The invention proves that the active ingredients of the venenum bufonis have strong affinity and are stably combined with target proteins such as PBP2a, BlaZ and AgrA of MRSA, so that the antibacterial effect of antibiotics is enhanced. The venenum bufonis extract and beta-lactam antibiotics are combined for use, so that a remarkable synergistic effect is achieved, and the drug resistance of drug-resistant staphylococcus aureus is overcome. Mouse model experiments prove that the venenum bufonis and penicillin G combined treatment has a remarkable treatment effect on MRSA infected mice. The combined application of the venenum bufonis and the penicillin G can effectively control the MRSA infection, and a novel and effective treatment scheme is provided.

This invention discloses the application of bufotoxin lactones in the preparation of anti-IHNV products, belonging to the field of biomedical technology. This invention is the first to discover and confirm that four bufotalin-based drugs (bufotalin, bufotalin ester, bufotalin, and bufotalin) have significant anti-IHNV effects: their IC50 values ​​against IHNV in vitro range from 0.0879 to 0.1809 μM, with selectivity indices all greater than 110. After 48 hours of treatment, they can reduce viral RNA expression by 110 to 173 times and viral titer by 4.36 to 5.15 lg; they also show broad-spectrum inhibitory effects against different genotypes of IHNV, such as LN-15, QH-17, and Blk94; in a rainbow trout in vivo model, after administration of a 0.5 mg / kg dose, the cumulative mortality rate decreased from 95% in the control group to 13.3% to 21.7%, with the highest relative protection rate reaching 85.96% (bufotalin), indicating that these compounds have highly efficient, broad-spectrum, and low-toxicity anti-IHNV activity and have excellent application prospects.

The toad venom preparation device comprises a base, a liquid accumulation tank is clamped to the middle of the upper end of the base, a connecting pipe is fixedly connected to the middle of the upper end of the liquid accumulation tank, and one end of the connecting pipe extends into an inner cavity of the liquid accumulation tank and is fixedly connected with a liquid outlet pipe; a guide mechanism used for guiding the position of the liquid outlet pipe is arranged in an inner cavity of the liquid accumulation tank, the guide mechanism comprises a driving block, the driving block is rotationally connected with the connecting pipe, and the driving block is fixedly connected with the upper end of the liquid outlet pipe. Through the arrangement of the sleeve, sputtered slurry can be blocked, slurry waste can be avoided while damage to collection personnel caused by slurry sputtering is avoided, and through sliding fit of the driven rod, the first arc-shaped groove and the second arc-shaped groove, the movement path of the lower end of the liquid outlet tank can be changed; the slurry can uniformly and dispersedly drop on the filter screen, so that the slurry is prevented from being accumulated at one place, and the quality and the speed of slurry filtration are not influenced.

The application discloses a kind of external use traditional Chinese medicine compound compositions for reducing sensitivity of glans penis and penis and prolonging intravaginal ejaculation latency, and the pharmaceutical composition includes the following weight fractions of components: 30-70 parts of gallnut, 0.2-3 parts of toad venom, 9-35 parts of pomegranate peel, 6-14 parts of cnidium, 9-35 parts of dandelion, 6-14 parts of sophora, 2-10 parts of mint, 0.5-4 parts of pepper.The traditional Chinese medicine compound composition provided by the application uses scientific proportioning, mutually coordinates and mutually promotes between components, and plays the role of synergistic effect.The external use traditional Chinese medicine compound of the application can significantly reduce the sensitivity of glans penis and penis, prolong intravaginal ejaculation latency, has definite clinical curative effect, is convenient to use, has good patient tolerance and compliance, is safe and has no side effect, long-term use is not found adverse reaction, and after being made into standard preparation, can promote the standardization treatment of external treatment of traditional Chinese medicine.

The application provides a live heart pill liposome aerosol and a preparation method and application thereof, the live heart pill liposome aerosol comprising drug-loaded liposomes, a propellant and a cosolvent; the drug-loaded liposomes comprise core materials and wall materials; the mass ratio of the core materials to the wall materials is 1:(5-11); the core materials comprise traditional Chinese medicine active ingredients; the wall materials comprise neutral lipids, anionic lipids and sterols; the traditional Chinese medicine active ingredients comprise 400-600 parts of ginseng, 150-300 parts of ox gall, 50-150 parts of ganoderma lucidum, 15-50 parts of pearls, 2-10 parts of aconite, 20-100 parts of safflower, 2-20 parts of musk, 5-15 parts of bear gall, 4-20 parts of toad venom and 2-10 parts of borneol in terms of weight parts. The live heart pill liposome aerosol of the application is administered through pulmonary inhalation, has a quick effect after administration and is obviously superior to an oral dosage form, and can better meet the timeliness requirement of first-aid medicines in the acute attack of angina pectoris.

This invention provides a method for rapidly determining the content of active ingredients in fresh toad venom extract using HLB solid-phase extraction-UV. This invention further provides the application of the above-mentioned method for rapidly determining the content of active ingredients in fresh toad venom extract using HLB solid-phase extraction-UV in the analysis of the quality of fresh toad venom extract. This invention also provides an analytical method for the quality of fresh toad venom extract. The method for rapidly determining the content of active ingredients in fresh toad venom extract using HLB solid-phase extraction-UV provided by this invention can purify and separate fresh toad venom extract using an HLB solid-phase extraction column to obtain a mixture of three components: bufotoxin, bufotoxin base, and bufotoxin ligand. The content of these components can be directly detected by ultraviolet spectrophotometer, which can rapidly characterize the quality of toad venom medicinal materials and provide a basis for the quality control of fresh toad venom.

The application discloses a method for extracting bufadienolides in bufonis medicinals and application thereof, and belongs to the technical field of effective component extraction of traditional Chinese medicines. The method uses a deep eutectic solvent (DES) as an extracting agent, and solves the problems of low purity of bufadienolides, poor extraction efficiency and complicated process in traditional water extraction or alcohol extraction methods by optimizing the solvent composition, material ratio and extraction process. The method has high extraction efficiency, can significantly improve the total content of bufalin, resibufogenin and cinobufagin in the extract, and is simple in process, environment-friendly and easy for large-scale industrial production, thereby providing a new approach for efficient utilization of bufonis medicinals.

The invention provides application of venenum bufonis active molecules in preparation of cGAS-STING pathway inhibitors, and particularly belongs to the technical field of protein inhibitors. Starting from a downstream interferon regulatory factor of a cGAS-STING pathway, the venenum bufonis active molecule is found to have relatively strong inhibitory activity on the cGAS-STING pathway through screening. The ulcerative colitis resisting activity evaluation on the active molecule arenobufagin of the venenum bufonis finds that the arenobufagin has a good relieving effect on the ulcerative colitis, the colon length can be obviously improved, and release of serum inflammatory factors of mice with the ulcerative colitis can also be inhibited. Finally, the venenum bufonis active molecule can be used as a cGAS-STING pathway inhibitor, plays a role in resisting ulcerative colitis, and lays a foundation for clinically developing medicines for treating ulcerative colitis.

The invention discloses a method for extracting toad steroid total lactone in toad medicinal materials and application of the toad steroid total lactone, and belongs to the technical field of extraction of effective components of traditional Chinese medicines. According to the method, a deep eutectic solvent (DES) is taken as an extracting agent, and the problems of low purity, poor extraction efficiency and tedious process of toad sterol total lactone in a traditional water extraction or alcohol extraction method are solved by optimizing the solvent composition, the material ratio and the extraction process. The method is high in extraction efficiency, the total content of bufalin, resibufogenin and cinobufagin in the extract can be remarkably increased, the process is simple and environmentally friendly, large-scale industrial production is easy, and a new way is provided for efficient utilization of toad medicinal materials.

The application belongs to the field of traditional Chinese medicine, and particularly relates to an external use delayed time towel agent and a preparation method thereof. The dried skin of the Chinese toad from which the toad venom has been scraped is selected, water extraction and alcohol extraction are combined, toad skin extract is efficiently extracted, and the toad skin extract is combined with male moth oil to prepare the delayed time towel agent. It is found that through the synergistic effect of the toad skin extract and the male moth oil, male premature ejaculation and erectile dysfunction can be significantly improved, and the synergistic effect of the two is much higher than that of single medicine, the medicinal effect is optimized, the core requirement of clinical application is met, the towel agent is prepared, independent packaging is achieved, and the towel agent has the characteristics of low cost, direct effect, simple operation, and difficulty in drug pollution.