47 results about "Bufotoxin" patented technology

The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to application of a traditional Chinese medicine composition in preparation of anti-hepatoma medicines. The traditional Chinese medicine composition comprises red ginseng, ox horn tip, musk, borneol, safflower, bezoar, radix aconiti lateralis praeparata slice, venenum bufonis, pseudo-ginseng, benzoin and pearl. Experimental results show that the traditional Chinese medicine composition can induce apoptosis and necrosis of liver cancer cells and inhibit proliferation of the liver cancer cells; and liver cancer cells are retarded to be in a G1 cell cycle. Therefore, the invention provides a new therapeutic drug for clinical treatment of liver cancer, and the raw materials are natural medicinal materials, have wide sources and have good application prospects.

This invention belongs to the field of traditional Chinese medicine, specifically relating to a topical delay spray and its preparation method. The invention uses the dried epidermis of the Huaxi toad (with the toad venom removed), and extracts it efficiently through a combination of water and alcohol extraction. Male silkworm moth oil can nourish and strengthen the body, tonify the liver and kidneys, and combat fatigue; studies have shown that male silkworm moth oil can effectively treat kidney yang deficiency. This invention combines the synergistic effects of toad skin extract and male silkworm moth oil to prepare a delay spray that can significantly treat premature ejaculation and erectile dysfunction in men. This preparation has the advantages of direct action, sustained release, avoidance of drug contact, and simple operation. This invention further improves the rational utilization system of toad resources, provides a practical solution for the efficient development of toad medicinal resources, and significantly reduces production and clinical application costs.

The application belongs to the field of traditional Chinese medicines, and particularly relates to a more low-irritation analgesic ancient prescription microemulsion gel and application thereof in anti-arthritis drugs. The application finds that after water-soluble and fat-soluble components of black plaster prepared from raw frankincense, myrrh, eaglewood, dragon's blood, Strychnos nux-vomica, Panax notoginseng, Oldenlandia diffusa, Venenum Bufonis, bovine bezoar, Stachys jamaicensis, Platycodon grandiflorum, Hirudo nipponia, Tinea, Solenopsis geminata, Scorpio, scorpio, red lead and sesame oil are extracted, a more low-irritation analgesic ancient prescription microemulsion gel is prepared; compared with the black plaster itself, the analgesic effect of the microemulsion gel is significantly improved, and the microemulsion gel has good anti-inflammatory effect and can be used for preparing anti-arthritis drugs; and the microemulsion gel has reduced toxicity and is more convenient to use.

The invention discloses an application of a traditional Chinese medicine composition in preparation of a medicine for preventing and treating chronic heart failure with cognitive impairment. The traditional Chinese medicine composition comprises the following components: ginseng, lucid ganoderma, safflower, musk, calculus bovis, bear gall, pearl, venenum bufonis, radix aconiti carmichaeli and borneol. The traditional Chinese medicine composition has a good treatment effect on qi deficiency and blood stasis type chronic heart failure accompanied with cognitive impairment, and a large number of test data prove that the traditional Chinese medicine composition can effectively improve the heart function, and / or cognitive function and / or traditional Chinese medicine symptoms of qi deficiency and blood stasis type chronic heart failure accompanied with cognitive impairment through reasonable compatibility of all the components, so that the traditional Chinese medicine composition has a good curative effect on qi deficiency and blood stasis type chronic heart failure accompanied with cognitive impairment. The symptoms of chronic heart failure with cognitive impairment are relieved from multiple aspects, and the progress of the disease course is blocked. The prescription is safe and reliable without toxic and side effects on human bodies.

This invention relates to an oral nanomedicine antigen delivery system and its construction method and application, belonging to the field of oral nanomaterials technology; the construction method includes the following steps: (1) preparation of OM nanoparticle suspension; (2) preparation of cationic liposome suspension; (3) preparation of cationic liposome suspension loaded with OM / BF; (4) construction of oral nanomedicine antigen delivery system. This invention breaks through multiple barriers in the gastrointestinal tract, improves drug bioavailability, and achieves precise immune tracking through the OVA tumor antigen model, simulating in vivo anti-tumor CTL response, thereby exerting anti-tumor effects synergistically at the cellular, tissue, and immune levels, effectively solving the problems of poor water solubility and low delivery efficiency of the active ingredient bufotoxin in traditional Chinese medicine. At the same time, through macrophage-mediated immune regulation and precise drug release, it improves targeting and treatment efficiency, opening up a new avenue for tumor immunotherapy with oral nanomedicine.

The invention relates to the technical field of venenum bufonis processing, in particular to venenum bufonis processing equipment and method.The venenum bufonis processing equipment comprises a base, a mechanical arm, a discharge promoting module, a flexible attaching module, an adsorption recycling module and a main controller. And the drainage promoting module is used for performing non-destructive stimulation on the posterior auricular gland to induce drainage. The flexible attaching module is provided with a flexible scraping head which can deform in a self-adaptive mode to be attached to the surface of a gland, and a flexible touch sensor array for sensing pressure distribution and a micro pneumatic cavity grid array for driving deformation are arranged in the flexible attaching module. The adsorption recovery module is integrated on the working face of the scraping head and is provided with a micro-suction hole network capable of independently controlling adsorption force in different areas. And the main controller coordinates all the modules to work in sequence: firstly promoting discharge, then controlling the flexible scraping head to perform scraping in a fitting state, meanwhile, intelligently adjusting adsorption force of different areas according to real-time pressure distribution so as to recover slurry, and constraining global contact force through a multi-dimensional force sensor so as to ensure safety.

The invention discloses a pure traditional Chinese medicine external delay spray for male premature ejaculation and a preparation method of the pure traditional Chinese medicine external delay spray, belongs to the technical field of medicine preparations, and can realize a delay effect on the premise of maintaining normal touch perception. The traditional Chinese medicine composition consists of the following components in parts by weight: 10-12 parts of herba epimedii; 10 to 12 parts of cynomorium songaricum; 8-10 parts of fructus cnidii; 8-10 parts of Chinese magnoliavine fruit; 8-10 parts of Chinese gall; 8-10 parts of semen allii tuberosi; 8 to 10 parts of herba agrimoniae; 2-5 parts of venenum bufonis; 9-11 parts of asarum; 10 to 12 parts of clove; 3-5 parts of polygala tenuifolia; 3-5 parts of radix astragali; 1-3 parts of mint; and 1-3 parts of borneol.

The present invention provides a method for quantitatively detecting the blood-transmitting components of Toad Venenum or Salvia miltiorrhiza in Musk Tongxin Dropping Pills, specifically comprising ultra-high performance liquid chromatography-tandem mass spectrometry detection of drug-containing plasma or tissue. The liquid chromatography conditions employ a C18-filled column, mobile phase A consisting of 0.05% to 0.2% formic acid in water, mobile phase B consisting of 0.05% to 0.2% formic acid in acetonitrile, and gradient elution; an electrospray ion source in positive ion mode, a drying gas temperature of 300 to 400°C, a drying gas flow rate of 4 to 8 L / min, a sheath gas temperature of 300 to 400°C, a sheath gas flow rate of 8 to 12 L / min, and a nebulizing gas pressure of 40 to 50 psi; a capillary inlet voltage of 3 to 4 kV; a cone voltage of 450 to 550 V, a collision energy of 10 to 30 eV, and a fragmentation voltage of 100 to 190 V. The present invention can simultaneously and quantitatively detect 9 kinds of toad venom components and 3 kinds of salvia miltiorrhiza components entering the blood in Musk Tongxin Dropping Pills, and has high detection sensitivity, good stability and short detection time, and is suitable for the pharmacokinetic study of Musk Tongxin Dropping Pills.

The invention provides a traditional Chinese medicine compound external preparation for invigorating the kidney and strengthening Yang and a preparation method thereof, belongs to the technical field of traditional Chinese medicine preparations, and aims to solve the problems that the bioavailability of the first-pass effect of existing oral administration is low, and effective components of external ointment are difficult to penetrate through the skin. The preparation is a composite gel containing a penetration enhancer and a temperature-sensitive gel matrix, and the traditional Chinese medicine compound at least comprises various active ingredients of male silkworm moth, centipede, epimedium, rhizoma curculiginis, cistanche, schisandra chinensis, semen cuscutae, polygala tenuifolia, fructus cnidii, semen allii tuberosi, actinolite, sulfur, venenum bufonis, amethyst and borneol. According to the preparation disclosed by the invention, the transdermal permeation rate and the cumulative permeation amount of various active ingredients in the compound are improved in order of magnitude, so that an effective treatment concentration can be ensured to be reached in an in-vitro local or in-vivo system. In clinical application, the traditional Chinese medicine composition has better effect taking speed and treatment effect than external application or oral administration of traditional prescriptions with the same name.

A compound anti-tumor traditional Chinese medicine composition, and a preparation method therefor and the use thereof. The compound anti-tumor traditional Chinese medicine composition comprises the following components in parts by weight: 300-600 parts of astragali radix, 80-120 parts of red ginseng, 4-10 parts of blister beetle and 1-4 parts of bufonis venenum. The combination of these components has the effects of clearing away heat and toxic materials, promoting blood circulation to remove blood stasis, softening hardness to remove stasis, and strengthening body resistance to eliminate pathogenic factors.

The application belongs to the field of biological pharmacy, and particularly discloses a preparation method of musk heart-activating dropping pills, which comprises the following steps: preparing a salvia miltiorrhiza clear paste, mixing the salvia miltiorrhiza clear paste, artificial musk, total ginsenosides of ginseng stems and leaves, toad venom, artificial ox gall, bear bile powder and borneol with polyethylene glycol 6000 and polysorbate 80, stirring and uniformly mixing, dropping, centrifuging, screening, drying and coating to prepare the musk heart-activating dropping pills. The application has remarkable clinical curative effect, no side effect, high drug stability, fast dissolution, high efficiency and rapid effect. The application further discloses a preparation device of the musk heart-activating dropping pills, wherein the vertical silica gel cleaning strips, the first silica gel cleaning strips and the second silica gel cleaning strips in the silica gel cleaning net frame and the silica gel cleaning strip component are cross arranged, can be in friction contact with different surfaces of the salvia miltiorrhiza in the cleaning process, and can provide comprehensive and detailed cleaning effect. The design not only increases the cleaning area, but also can effectively remove the small dust and impurities on the surface of the salvia miltiorrhiza, and avoids the dead angle which is easily missed in the traditional cleaning mode.

The invention discloses eye protection liquid and a preparation method thereof, and belongs to the technical field of traditional Chinese medicine external preparations. The eye protection liquid is prepared from a chrysanthemum extract, borneol, calculus bovis factitius, venenum bufonis, realgar, a mint extract, a pipewort extract, a feather cockscomb seed extract, a butterflybush flower extract, pearl powder and purified water according to a specific weight part ratio, and is prepared by soaking, distilling, filtering and filling. The traditional Chinese medicine composition can rapidly permeate into eyes and directly enter focus, can relieve and treat various eye diseases such as presbyopia, myopia, amblyopia, cataract and the like, has the effects of soothing liver and relieving depression, promoting blood circulation and dredging collaterals, tonifying kidney and nourishing yin, supplementing optic nerve nutrition and improving microcirculation around the eyes, has the total effective rate of 100% and the cure rate of 80-95%, and is free of side effects and drug resistance and convenient to use.

This invention discloses a composition and its preparation method for treating human papillomavirus (HPV) and cervical intraepithelial neoplasia (CIN), belonging to the field of traditional Chinese medicine technology. The formula consists of 6-15 parts of American cockroach, 5-11 parts of hawthorn, 5-11 parts of chicory, 3-9 parts of *Scutellaria baicalensis*, 8-20 parts of *Sophora flavescens*, and 6-15 parts of *Cnidium monnieri*, and also contains 0.01-1 parts by weight of toad venom. The dosage form is one or more of the following: external gel, rinse, foam, suppository, and film preparation. It has high safety and is suitable for HPV infection and CIN changes, solving the problem of poor efficacy in existing treatments.

The application belongs to the technical field of traditional Chinese medicines, and particularly relates to application of a traditional Chinese medicine composition in preparation of a liver cancer resisting medicine. The traditional Chinese medicine composition comprises red ginseng, ox horn, musk, borneol, safflower, ox gall, appendices, toad venom, panax notoginseng, storax and pearl. Experimental results show that the traditional Chinese medicine composition can induce apoptosis and necrosis of liver cancer cells, inhibit proliferation of the liver cancer cells, and block the liver cancer cells in the G1 phase of the cell cycle. Therefore, the application provides a new treatment medicine for clinical treatment of liver cancer, and the raw materials are natural medicinal materials, are widely sourced, and have a good application prospect.

This invention belongs to the field of biomedical technology, specifically relating to an ointment and its application. The toad oil used in this invention differs significantly in composition from toad venom, and its ointment is effective for various acute and chronic pain conditions. Animal experiments have confirmed that the toad oil ointment significantly reduces the frequency and delays the onset of acetic acid-induced writhing responses, demonstrating a clear analgesic effect. Clinical studies have shown that this ointment is rapidly effective and has a definite therapeutic effect on acute gout attacks, postherpetic neuralgia, and arthritis-related pain and swelling. The formulation effectively improves drug retention and user comfort, and no skin irritation has been observed. This development achieves comprehensive utilization of toad resources, providing an example for the sustainable development of traditional Chinese medicine and the efficient utilization of biological resources.

The invention relates to the technical field of traditional Chinese medicine, in particular to a traditional Chinese medicine composition for gastroesophageal reflux disease, a preparation, a preparation method and application. The traditional Chinese medicine composition is prepared from the following raw medicinal materials in parts by weight: 20 to 30 parts of radix bupleuri, 20 to 30 parts of rhizoma polygoni cuspidati, 10 to 15 parts of caulis bambusae in taeniam, 20 to 25 parts of ginger processed pinellia, 15 to 20 parts of fructus evodiae, 20 to 25 parts of rhizoma atractylodis macrocephalae, 15 to 20 parts of poria cocos, 10 to 20 parts of rhizoma cyperi, 10 to 15 parts of spica prunellae, 10 to 15 parts of venenum bufonis, 10 to 20 parts of wine-processed radix paeoniae alba, 10 to 15 parts of caragana sinica rehd, 10 to 15 parts of fructus chebulae and 10 to 15 parts of dens draconis. The preparation method comprises the following steps: weighing the traditional Chinese medicine composition according to the formula dosage, and then adding water for decoction to obtain a traditional Chinese medicine decoction; combining the traditional Chinese medicine decoction, filtering and concentrating to obtain traditional Chinese medicine extract; and adding auxiliary materials into the traditional Chinese medicine extract, and preparing by adopting a conventional preparation mode. All the monarch, minister, assistant and guide medicines are combined to well treat the gastroesophageal reflux disease.

The invention discloses a toad venom collecting and processing method, and belongs to the technical field of traditional Chinese medicine. The toad venom collecting and processing method comprises the following steps: (1) before toad venom collection, adjusting the state of toads, including environment adjustment, feed adjustment and toad individual screening; (2) performing bioelectrical stimulation on the selected toads, collecting and filtering toad venom serous fluid; disinfecting and repairing the wound of the toad after the toad venom is collected; and (3) extracting and separating the collected venenum bufonis serous fluid to obtain the venenum bufonis with high active ingredient content. According to the method, the venenum bufonis is collected and processed on the basis of physiological status optimization before collection, bioelectrical stimulation during collection and an aqueous two-phase extraction technology after collection, on one hand, non-invasive collection and green separation are achieved, and the content of effective components in the venenum bufonis is increased; on the other hand, sustainable and full utilization of toads can be achieved, the secretion amount of the venenum bufonis is stable, and large-scale utilization can be achieved.

This invention discloses a library of traditional Chinese medicine (TCM) compositions based on general and local anesthesia mechanisms, along with various dosage forms and preparation methods. The invention constructs a systematic library of TCM anesthetic compositions: the general anesthetic composition consists of extracts from herbs such as Datura stramonium, Datura stramonium, processed Aconitum carmichaelii, processed Aconitum kusnezoffii, Saussurea involucrata, Jasminum sambac root, Rhododendron molle, and Sanqie Sanqie; the local anesthetic composition consists of extracts from herbs such as Asarum heterotropoides, Murraya paniculata, Ephedra sinica, Toad venom, and Corydalis yanhusuo. This invention further develops the above compositions into various modern dosage forms, including injections, tablets, capsules, and sprays. In particular, this invention innovatively introduces a clove-cinnamon natural preservative and flavoring system to replace chemical preservatives. In terms of preparation technology, advanced technologies such as supercritical CO2 extraction, microwave-assisted extraction, nano-embedding, and nanoemulsions are integrated, and a self-extraction scheme is adopted to achieve controllable quality and cost. Simultaneously, a quality detection method based on HPLC fingerprinting is established. This invention achieves full-scenario coverage of anesthesia and analgesia, from general to local, from emergency to routine care, with outstanding advantages of safety, effectiveness, and controllable quality.

The utility model discloses a multifunctional toad venom collector which comprises a support and a protective cover, the protective cover is fixedly connected with the support, the upper surface of the protective cover is fixedly connected with a mounting frame, one surface of the mounting frame is fixedly connected with a motor, the output end of the motor is fixedly connected with a screw rod, and the peripheral side face of the screw rod is provided with a moving block. The bottom face of the moving block is fixedly connected with an electric push rod, the output end of the electric push rod is fixedly connected with a connecting plate, a plurality of guide grooves are formed in the upper surface of the connecting plate, guide rods are installed in the guide grooves, springs are installed on the circumferential side faces of the guide rods, the bottom ends of the guide rods are fixedly connected with an extrusion soft plate, and a mesh plate is fixedly connected into the support. The toad collecting device solves the problems that in the prior art, toads are directly grabbed by hands and collected through a scraper, and the collecting method is prone to causing the fact that serous fluid is splashed into the eyes and harms the eyes.

The invention belongs to the technical field of cultivation, and particularly relates to intercropping, in particular to a toad and earthworm intercropping system and intercropping method based on towel gourd plant.The intercropping system comprises an intercropping land selected as an intercropping area, and peripheral supporting rods are arranged around the intercropping land; the lower portions of the peripheral supporting rods are provided with edge-proof fences which enclose the intercropping area into a space with the lower portion closed, towel gourd climbing frames are erected between the tops of the peripheral supporting rods, breeding ditches are formed in the closed space defined by the edge-proof fences and close to the edge-proof fences, and a blocking net is arranged above a middle area defined by the breeding ditches. Loofah climbing rods are arranged in the intercropping land at intervals. According to the intercropping system disclosed by the invention, the characteristics and advantages of the towel gourds, the toads and the earthworms are brought into full play through spatial layering, resource circulation and biological isolation by re-dividing and re-arranging a breeding area, and the economic value is maximized. Meanwhile, under the culture system, the content of venenum bufonis in toad bodies can be remarkably increased.

The invention discloses a traditional Chinese medicine composition for treating wound ulcer and a preparation method thereof, and belongs to the technical field of traditional Chinese medicines. The prescription is composed of 5-15 parts of slender gouania root, 5-10 parts of maggot, 0.01-0.5 part of venenum bufonis, 5-10 parts of American cockroach, 5-15 parts of abelmoschus manihot, 3-9 parts of rheum officinale, 1-5 parts of safflower and 5-15 parts of fructus chebulae, the dosage form is external ointment, powder, oil, liquid preparation and the like, the traditional Chinese medicine composition is remarkable in curative effect, the cure rate can reach 85% or above after 2 months of treatment, the average healing time is shortened by 40 days or above, safety is high, and the traditional Chinese medicine composition is suitable for clinical application. The traditional Chinese medicine composition is suitable for chronic intractable wound ulcer such as diabetic foot ulcer, and the problem of poor treatment effect in the prior art is solved.

The application belongs to the field of traditional Chinese medicine, and particularly relates to an external use delayed oil agent and a preparation method thereof. The dried skin of the Visternotus limnocharis which has scraped toad venom is combined with the male silkworm moth which has the functions of tonifying liver and kidney, strengthening yang and astringing essence to significantly improve male premature ejaculation and erectile dysfunction through water extraction and alcohol extraction. The male silkworm moth oil has unique promotion potential and development potential. The application combines the toad skin and the male silkworm moth oil to prepare the delayed oil agent, significantly improves male premature ejaculation and erectile dysfunction, and has the characteristics of direct action and simple operation. The preparation cost is low.

The invention discloses a traditional Chinese medicine preparation for treating chronic esophagitis and esophageal precancerous lesions and a preparation method thereof. The traditional Chinese medicine preparation is prepared from nux vomica, sal ammoniac, sculellaria barbata, Chinese lobelia, duchesnea indica, marsdenia tenacissima, solanum septemlobum bunge, selfheal, processed pinellia ternate, ginger, coptis chinensis, sticktight, airpotato yam, radix clematidis, borax, platycodon grandiflorum, hawthorn, kelp, seaweed, edible tulip and venenum bufonis. The preparation method comprises the following steps: processing or performing raw treatment on the materials, grinding into fine powder by using a grinder, finely screening by using a sieve, removing coarse stems, and mixing in parts by weight to obtain a finished product of the preparation. The traditional Chinese medicine is simple in preparation method and low in cost, can dissipate blood stasis, regulate liver and stomach and nourish stomach yin, and can effectively treat chronic esophagitis and esophageal precancerous lesions.

The utility model discloses a venenum bufonis decompression drying room, and relates to the technical field of traditional Chinese medicinal material processing, and the venenum bufonis decompression drying room is technically characterized by comprising a room body, a plurality of placing plates are fixedly installed on the inner walls of the two sides of the room body, a rotating pipe is rotatably installed in the room body in a penetrating mode, and a plurality of connecting pipes communicated with the interior are fixedly installed on the outer wall of the rotating pipe; the ends of the two connecting pipes on the same side are jointly and fixedly connected with a drying pipe, the outer walls of the two drying pipes are each provided with a plurality of air outlet holes communicated with the interior, and a connecting sleeve is fixedly installed on the outer wall of the rotating pipe. The first motor can drive the rotating pipe to rotate through the two transmission wheels connected through the belt, and then the rotating pipe drives the two drying pipes to rotate through the connecting pipe, so that the rotating pipe can rotate while warm air is blown out, the warm air can be uniformly distributed in the room body, and the venenum bufonis placed in the room body can be more uniformly dried.

The present application provides a method for constructing the fingerprint of Xinbao pills, comprising the following steps: 1) preparation of test sample solution and mixed control solution; 2) construction of the fingerprint of Xinbao pills: taking the test sample solution and the mixed control solution respectively, and performing high performance liquid chromatography detection to obtain the fingerprint of Xinbao pills. The present application belongs to the technical field of Chinese medicine preparation detection, and provides a method for constructing the fingerprint of Xinbao pills, which is simple and easy to operate, has good precision, stability and repeatability, can realize the detection of multiple components of Xinbao pills, and better reflects the overall quality of Xinbao pills; meanwhile, the constructed HPLC fingerprint of Xinbao pills has rich detection components, 21 common characteristic peaks are determined, and the anti-heart failure active components in ginseng, panax notoginseng, toad venom and datura stramonium in Xinbao pills are covered, which can provide a reference for comprehensively controlling the internal quality of Xinbao pills and further ensure the clinical efficacy of the product.

The invention provides application of a venenum bufonis extract combined with beta-lactam antibiotics in medicines for treating MRSA infection, and belongs to the technical field of biological medicines. The invention proves that the active ingredients of the venenum bufonis have strong affinity and are stably combined with target proteins such as PBP2a, BlaZ and AgrA of MRSA, so that the antibacterial effect of antibiotics is enhanced. The venenum bufonis extract and beta-lactam antibiotics are combined for use, so that a remarkable synergistic effect is achieved, and the drug resistance of drug-resistant staphylococcus aureus is overcome. Mouse model experiments prove that the venenum bufonis and penicillin G combined treatment has a remarkable treatment effect on MRSA infected mice. The combined application of the venenum bufonis and the penicillin G can effectively control the MRSA infection, and a novel and effective treatment scheme is provided.

This invention discloses the application of bufotoxin lactones in the preparation of anti-IHNV products, belonging to the field of biomedical technology. This invention is the first to discover and confirm that four bufotalin-based drugs (bufotalin, bufotalin ester, bufotalin, and bufotalin) have significant anti-IHNV effects: their IC50 values ​​against IHNV in vitro range from 0.0879 to 0.1809 μM, with selectivity indices all greater than 110. After 48 hours of treatment, they can reduce viral RNA expression by 110 to 173 times and viral titer by 4.36 to 5.15 lg; they also show broad-spectrum inhibitory effects against different genotypes of IHNV, such as LN-15, QH-17, and Blk94; in a rainbow trout in vivo model, after administration of a 0.5 mg / kg dose, the cumulative mortality rate decreased from 95% in the control group to 13.3% to 21.7%, with the highest relative protection rate reaching 85.96% (bufotalin), indicating that these compounds have highly efficient, broad-spectrum, and low-toxicity anti-IHNV activity and have excellent application prospects.

The invention relates to the technical field of sustained-release veterinary preparations, in particular to a heat-clearing and detoxifying sustained-release Niuchan preparation and a preparation method thereof. The sustained-release Niuchan preparation capable of clearing away heat and toxic materials comprises the following raw materials in parts by mass: 1-3 parts of venenum bufonis, 3-6 parts of calculus bovis factitius, 1-5 parts of a Chinese herbal medicine extract, 1-5 parts of beta-cyclodextrin, 90-100 parts of dextrin, 700-800 parts of cane sugar and 1-3 parts of a borneol ethanol solution. The Chinese herbal medicine extract is prepared by the following steps: adding scutellaria baicalensis and liquorice into water, vacuumizing to-0.04 to-0.06 MPa, keeping for 5-10 minutes, performing reflux boiling at 60-80 DEG C for 2-6 hours, and filtering to obtain an extracting solution a and filter residues a; adding an ethanol water solution into the filter residue a, carrying out reflux extraction at 60-70 DEG C for 1-3 hours, and filtering to obtain an extracting solution b; combining the extracting solution a and the extracting solution b, then adding dendritic polylysine, stirring for 1-2 hours at 40-50 DEG C under the protection of nitrogen, carrying out homogenizing cycle treatment for 2-4 times, and concentrating.

The invention provides an application of a venenum bufonis stock solution in preparation of a male delay product. The venenum bufonis is creatively dried, crushed and heated, and the venenum bufonis reacts with ethanol and is subjected to suction filtration, so that the skin sensitivity of the male penis is reduced, the comprehensive effect of delaying is achieved, and the venenum bufonis composition is mild and safe, has no adverse reaction, is easily available in raw material source and has market value.

The present invention discloses a preparation method, a formula and an application of medicated ink for preventing or treating thyroid nodules. The medicated ink comprises the following raw materials in parts by weight: concentrated powder of buffalo horn, antelope horn 270-290 parts, musk 210-230 parts, pearl, borneol, bufotoxin 130-150 parts, bezoar 13-15 parts, cinnabar, ox gallbladder, bear gallbladder, snake gallbladder, pig gallbladder, black carp gallbladder, lotus node, safflower, small thistle, large thistle, rhizoma lmperatae, selfheal spike, cortex moutan, clove, ligusticum wallichii. Experimental studies show that the medicated ink has obvious curative effects on preventing and treating thyroid nodules. The present invention provides an effective solution for the treatment or prevention of thyroid nodules.