352 results about "Thin-layer chromatography" patented technology

The invention discloses a tea oil adulteration detection method based on thin-layer chromatography and image analysis, and belongs to the technical field of tea oil adulteration detection.The tea oil adulteration detection method comprises the steps that firstly, tea oil with different adulteration concentrations is analyzed based on thin-layer chromatography, oil sample images are obtained, and therefore an image recognition database is established; the adulterated tea oil lipid and lipid adjunct with different concentrations have different color development positions under the action of the composite organic solvent, so that the adulterated tea oil machine identification model is constructed by taking different color development positions as characteristic indexes. And identifying a thin-layer chromatogram of a to-be-detected oil sample by adopting the tea oil machine identification model, and judging the adulteration condition of the tea oil according to an identification result. According to the method, the thin-layer chromatography analysis and the machine algorithm are combined, and the thin-layer chromatogram of the sample to be detected is identified and distinguished by utilizing the machine algorithm, so that the tea oil adulteration result and adulteration concentration can be obtained, and the method is simple, convenient and rapid to operate and easy to popularize and promote.

The invention discloses a substance-based quality detection method for Xiaohuang powder, and belongs to the technical field of quality detection of traditional Chinese medicines. According to the method, standard control of a classic famous prescription is realized by constructing a multi-dimensional quality control system. The method comprises the following steps: firstly, carrying out specific identification on gardenia, divaricate saposhnikovia root, liquorice and pogostemon cablin in the prescription by adopting a thin-layer chromatography; meanwhile, a multi-index component content determination method is established, and the contents of five components including geniposide, cimicifugin, 5-O-methylvisammioside, liquiritin and glycyrrhizic acid are synchronously determined by applying a high performance liquid chromatography (HPLC). Through a system quality control strategy combining qualitative identification and quantitative analysis, not only is a quality standard system of the substance reference of the Xiaohuang powder perfected, but also reliable technical guarantee is provided for ensuring the stability and consistency of product quality. The establishment of the method establishes a standardized method system for the substance-based quality control of the classical famous prescription Xiaohuang powder.

The preparation method of the flavone enantiomer compound comprises the following steps: crushing epimedium sagittatum to prepare paste, suspending with water, extracting with petroleum ether, dichloromethane, ethyl acetate and n-butyl alcohol respectively to obtain extractum of each part, carrying out gradient elution separation by using silica gel column chromatography, and concentrating to obtain the flavone enantiomer compound extracted from epimedium sagittatum. Combining the similar parts according to a thin layer chromatography (TLC) color development result to obtain a plurality of polar segments; carrying out chromatography and gradient elution on the target polar section to obtain a plurality of components, and then combining the similar components through thin-layer chromatography (TLC) dot plate absorption to obtain a plurality of sub-components; a target component is eluted and purified, fractions with different retention times are collected and dried, and four compounds are obtained respectively, have a remarkable inhibition effect on the cell viability of human breast cancer cells MCF-7, have anti-breast cancer activity, can be applied to preparation of drugs for treating breast cancer, and have huge social and economic benefits.

The invention discloses a synthesis method of a novel benzosuberone compound, and belongs to the field of organic synthesis. The preparation method comprises the following steps: uniformly stirring methyltriphenylphosphonium bromide in a solvent at room temperature, dropwise adding an n-hexane solution of n-butyllithium in an ice-water bath, stirring a reaction system at room temperature for 30-40 minutes after dropwise adding is completed, then adding an initial raw material phenanthrenequinone 1 in the ice-water bath, transferring the reaction system to 40 DEG C for reaction, and after the reaction is monitored to be complete by a TLC (Thin Layer Chromatography) dot plate, filtering, washing and drying to obtain the phenanthrenequinone 1. And pouring the reaction liquid into a silica gel chromatographic column, and simply filtering by using dichloromethane as an eluent to remove salt and phosphine oxide. And performing rotary evaporation to remove the organic solvent to obtain a crude product, and separating and purifying to obtain a target product 2. The method is short in reaction time and low in reaction cost, provides a practical method for obtaining the seven-membered rigid ring skeleton, and has potential value for synthesis of natural products and bioactive molecules.

The invention provides a specific chromatogram construction and content determination method of the radix asparagi and radix ophiopogonis decoction preparation and a thin-layer chromatography identification method of traditional Chinese medicine flavor components in the radix asparagi and radix ophiopogonis decoction preparation. The specific chromatogram construction method of the radix asparagi decoction preparation comprises the following steps: step 1, taking mangiferin, quercetin-3-O-glucuronide, baicalin, wogonoside and ammonium glycyrrhizinate reference substances, and adding a solvent to prepare a reference substance solution; step 2, taking an Eupatorium japonicum-Liupu decoction reference sample, and adding a solvent to prepare a test solution; and 3, respectively detecting the reference substance solution and the test solution by using high performance liquid chromatography, and by taking the characteristic spectrum of the reference substance solution as a reference spectrum, selecting a common peak from the characteristic spectrum of the test solution to construct the characteristic spectrum of the radix asparagi decoction preparation.

The invention relates to the technical field of traditional Chinese medicine detection, in particular to a quality control method of a heart fire clearing lotus seed beverage compound preparation, which comprises the following steps: respectively preparing test solution A-E; based on the test solution A, identifying the scutellaria baicalensis by adopting a thin-layer chromatography; identifying cortex lycii radicis and liquorice based on the test solution B, identifying ginseng based on the test solution C, identifying semen plantaginis based on the test solution D, and identifying lotus seeds based on the test solution E. According to the method, repeated extraction of the reference sample during identification of different medicine flavors can be reduced, and the method has the advantages of clear spots, good separation degree, strong specificity, no background interference and good durability.

The invention discloses a method for preparing a biological hydrocarbon compound by organic photocatalysis higher fatty acid decarboxylation, which comprises the following steps: adding higher fatty acid, a hydrogen atom transfer catalyst and an acridine photocatalyst / solid-phase acridine photocatalyst into a reaction solvent, stirring and reacting for 12-24 hours under the conditions of blue light irradiation and room temperature to obtain a reaction solution, filtering, washing and drying to obtain the biological hydrocarbon compound. And removing the reaction solvent of the obtained reaction solution under reduced pressure, and purifying by thin layer chromatography / column chromatography to obtain the long-chain alkane compound after decarboxylation of the higher fatty acid. The method is simple in step, mild in reaction condition and high in product yield, does not use any metal compound, and has the characteristics of high atom economy and environmental protection. And for unsaturated carboxylic acid, olefin configuration before and after decarboxylation is not influenced. When solid-phase acridine is used as a photocatalyst for reaction, the catalyst can be recycled through simple washing, filtering and recycling, excellent catalytic activity can still be kept after the catalyst is recycled for more than 10 times, and the reaction cost is further reduced.

The invention discloses a method for synthesizing 3, 3 '-difluorobenzene. The invention discloses a method for preparing 3, 3 ', 5, 5'-tetramethyl-4, 4 '-dihydroxybiphenyl and derivatives thereof, which comprises the following steps of: adding 1.0 mmol of 4-halogenated aryl phenol compound, 0.05 mmol of photocatalyst and a solvent into a reaction container in a nitrogen atmosphere, stirring until the materials are dissolved, adding 0.05 mmol of metal catalyst and 0.06 mmol of ligand which are prepared in advance, turning on a light source, stirring for 24 hours at room temperature, performing thin layer chromatography detection reaction, filtering, washing and drying to obtain the 3, 3', 5, 5 '-tetramethyl-4, 4'-dihydroxybiphenyl and derivatives thereof. And after the reaction is finished, filtering the reaction liquid, removing the solvent through rotary evaporation, and carrying out recrystallization or column chromatography by using a mixed solvent to obtain the target product 3, 3 ', 5, 5'-tetramethyl-4, 4 '-dihydroxybiphenyl and the derivative thereof. According to the method, the 4-halogenated aryl phenol compound is used as a raw material, the required compound is obtained in one step through'nickel + light 'catalysis, and waste and related pollution caused by the use of high-explosive gas or a reducing agent are not needed; and a recyclable photocatalyst is used, so that the method is more environment-friendly and has a good industrial application prospect.

The invention discloses a radix astragali, rhizoma polygonati and ligustrum lucidum ait traditional Chinese medicine preparation for treating myelosuppression as well as a preparation method and a detection method thereof. The preparation is prepared from 50-150 parts of radix astragali, 50-150 parts of wine-processed rhizoma polygonati, 25-75 parts of wine-processed ligustrum lucidum ait, 50-150 parts of blanched rhizoma drynariae, 50-150 parts of radix trichosanthis and 25-75 parts of herba epimedii. The optimal extraction process and refining process are screened out based on effective components and pharmacological activity through a large number of tests, and the optimal preparation process of the Shuanghuangliangwhitening oral liquid and other traditional Chinese medicine preparations is obtained through stability investigation and verification. According to the invention, a thin layer chromatography is adopted, the four medicinal materials of astragalus membranaceus, glossy privet fruit, rhizoma drynariae and herba epimedii are respectively subjected to thin layer chromatography research, and methodology research shows that the method is stable and feasible. The invention establishes a content determination method for astragaloside, calycosin-7-glucoside, specnuezhenide and icariin, establishes a quality standard, and has important significance for ensuring the clinical curative effect and safety of astragaloside, calycosin-7-glucoside, specnuezhenide and icariin.

The invention relates to the technical field of pharmaceutical analysis, in particular to a thin-layer chromatography identification method of vitality-preserving decoction and a preparation thereof. Comprising the following steps: S1, extracting a sample to be detected by using diethyl ether, and preparing a test solution; s2, preparing a cinnamaldehyde reference substance solution and a 6-gingerol reference substance solution; the method further comprises at least one of the steps S3 and S4; s3, dripping the test solution and the cinnamaldehyde reference solution on the same thin-layer plate, developing with a first developing solvent, and identifying the cinnamon; the first developing solvent comprises petroleum ether and ethyl acetate in a volume ratio of (15-20): 3; s4, dripping the test solution and the 6-gingerol reference substance solution on the same thin-layer plate, developing with a second developing solvent, and identifying the ginger; the second developing solvent is prepared from petroleum ether, trichloromethane and ethyl acetate according to the volume ratio of (2-4): (1-2): (1-3). The method is good in overall separation effect, clear in spots, good in specificity and durability, and capable of identifying the medicine flavors of cinnamon and ginger in the vitality-preserving decoction and the preparation thereof.

The invention relates to the technical field of thin-layer chromatography detection, and discloses a thin-layer chromatography detection method for free fluorine-18 in a fluorine-18 labeled radiopharmaceutical, which adopts ethyl acetate and n-hexane as developing solvents, and the volume ratio of ethyl acetate to n-hexane is (50-90): (10-50). The developing solvent with a specific volume ratio is adopted, and the two key indexes that the Rf value of the marked active ingredient in the radiopharmaceutical is smaller than 0.8 and the separation degree (the separation degree of the marked active ingredient in the radiopharmaceutical and free fluorine-18) R is larger than 1 are considered.

The invention belongs to the technical field of quality control of traditional Chinese medicines, and particularly relates to a specific chromatogram construction method and a quality control method of radix astragali and cassia twig five-material decoction, and the quality control method comprises a specific chromatogram identification method and a thin-layer chromatography identification method. The UPLC is used for construction and identification of the characteristic spectrum, and the method is short in detection time, high in specificity, rapid, efficient and low in cost. According to the specific chromatogram, 17 common characteristic peaks are stipulated, 12 characteristic peaks are identified, the identification rate is high, and analysis and identification of the material basis of the radix astragali and cassia twig five-material decoction are facilitated. According to the thin-layer chromatography identification method disclosed by the invention, a novel thin-layer identification method for identifying two medicines (astragalus membranaceus and radix paeoniae alba) in a compound by quantitative analysis of multi-components by single marker is successfully created, the detection conditions are simple, rapid and efficient, the specificity is high, the cost is relatively low, and the practicability is relatively high.

The invention discloses a quality detection method of an infantile poria cocos and coix seed paste, the detection method sequentially comprises the steps of preparation of the infantile poria cocos and coix seed paste, thin-layer identification, finished product inspection and content determination, and according to inspection items (general rule 0183) under oral thick paste items of four parts of Chinese Pharmacopoeia of 2020 edition, inspection items of characters, relative density, insoluble substances and water content are studied; identifying poria cocos, semen euryales and semen coicis in the root-benefiting conditioning paste by adopting a thin layer chromatography (TLC); according to the method, the content of pachymic acid serving as an effective component in the poria cocos and coix seed paste for children is measured and researched by adopting an HPLC (High Performance Liquid Chromatography) method, so that a basis is provided for quality control of the product, and the method is good in reproducibility, good in durability, simple and convenient, and capable of effectively controlling the quality of the poria cocos and coix seed paste for children.

The invention relates to a method for detecting a clearing agent main agent in gasoline, and the method comprises the following steps: carrying out thin-layer chromatography detection on a to-be-detected sample, and determining the composition of the clearing agent main agent in the concentrated to-be-detected sample according to color spot distribution on an obtained thin-layer chromatography plate, wherein the clearing agent main agent comprises one or more of a polyether amine clearing agent, a polyisobutene amine clearing agent and a Mannich base clearing agent; the thin-layer chromatography detection sequentially comprises developing and developing treatment; a developing agent for developing comprises one or more of a developing agent I, a developing agent II and a developing agent III; the developing solvent I is methanol, and the developing solvent II and the developing solvent III comprise one or more of alcohol with the carbon atom number of 1-6, aliphatic hydrocarbon with the carbon atom number of 5-12 and cycloalkane with the carbon atom number of 5-12. According to the method provided by the invention, whether the clearing agent main agent is added into the sample to be detected or not can be rapidly determined, and the type of the clearing agent main agent can be rapidly and accurately detected.

System (100) for the synthesis of new methylsulfonylacrylimidamide analogues, comprising: a) a reaction vessel unit (102) configured to maintain a controlled reaction environment; b) a reagent supply unit (104) configured to introduce the following sequentially: - Propargyl bromide - secondary amine, - Triethylamine, - Water, - Mesylazide and - Copper iodide catalyst; c) a reaction monitoring unit (106) configured to: - to detect the development of nitrogen gas, and - to monitor the completion of the reaction using thin-layer chromatography; d) a temperature control unit (108) configured to maintain room temperature conditions during the reaction; and e) a stirring unit (110) in conjunction with the reaction vessel unit (102) configured to ensure continuous stirring of the reaction mixture.

Front-Interface Thin Layer Chromatography Chamber

The present invention discloses a thin layer chromatography identification method for simultaneously identifying multiple components in a refined flat peach pill preparation and its application, which belongs to the technical field of quality control of traditional Chinese medicine. The thin layer chromatography identification method of the present invention is divided into two parts. The first part adopts methanol ultrasonic extraction and is developed by toluene-ethyl acetate-formic acid-methanol system, which can simultaneously identify the alpinia oxyphylla, wolfberry and fenxinmu in the refined flat peach pill; the second part is based on the methanol ultrasonic extraction, and hydrochloric acid is added to heat and reflux extraction, shaken and evaporated, and then ethanol is redissolved and developed by petroleum ether-ethyl acetate system, which can identify the ophiopogon in the refined flat peach pill. That is, only two sample preparation methods are required, and one thin layer plate can simply, quickly and effectively identify the four medicinal materials in the refined flat peach pill, and the color spots are clear, without tailing, without cross-linking and good specificity.

The present application provides a kind of Echinops formula granule quality detection method, including the thin layer identification of Echinops formula granule, characteristic map construction and β-ecdysterone content determination, the content standard of formula granule is limited to every 1g contains β-ecdysterone 3.5~14.4mg, thin layer identification is identified using thin layer chromatography, characteristic map construction and β-ecdysterone content determination are all using liquid chromatography method.The quality detection method of Echinops formula granule of the present application, by the detection of thin layer identification, characteristic map construction and β-ecdysterone content determination to assess the quality of Echinops formula granule, can comprehensively reflect the inherent quality of formula granule and better control the quality of Echinops formula granule.

The application discloses a kind of radionuclide 18 The present application discloses a method for detecting the radiochemical purity of F-labeled FAPI compounds, which comprises using thin layer chromatography to detect the radiochemical purity of F-labeled FAPI compounds 18 The present application discloses a method for detecting the radiochemical purity of F-labeled FAPI compounds, which comprises using thin layer chromatography to detect the radiochemical purity of F-labeled FAPI compounds 18 The present application discloses a method for detecting the radiochemical purity of F-labeled FAPI compounds, which comprises using thin layer chromatography to detect the radiochemical purity of F-labeled FAPI compounds 18 The present application discloses a method for detecting the radiochemical purity of F-labeled FAPI compounds, which comprises using thin layer chromatography to detect the radiochemical purity of F-labeled FAPI compounds 18 The present application discloses a method for detecting the radiochemical purity of F-labeled FAPI compounds, which comprises using thin layer chromatography to detect the radiochemical purity of F-labeled FAPI compounds 18 The present application discloses a method for detecting the radiochemical purity of F-labeled FAPI compounds, which comprises using thin layer chromatography to detect the radiochemical purity of F-labeled FAPI compounds 18 The present application discloses a method for detecting the radiochemical purity of F-labeled FAPI compounds, which comprises using thin layer chromatography to detect the radiochemical purity of F-labeled FAPI compounds 18 The present application discloses a method for detecting the radiochemical purity of F-labeled FAPI compounds, which comprises using thin layer chromatography to detect the radiochemical purity of F-labeled FAPI compounds 18 The present application discloses a method for detecting the radiochemical purity of F-labeled FAPI compounds, which comprises using thin layer chromatography to detect the radiochemical purity of F-labeled FAPI compounds 18 The present application discloses a method for detecting the radiochemical purity of F-labeled FAPI compounds, which comprises using thin layer chromatography to detect the radiochemical purity of F-labeled FAPI compounds 18 The present application discloses a method for detecting the radiochemical purity of F-labeled FAPI compounds, which comprises using thin layer chromatography to detect the radiochemical purity of F-labeled FAPI compounds

The invention relates to the technical field of thin-layer chromatography detection, and discloses a thin-layer chromatography separation method for amino polyether and a matrix in a fluorine-18 labeled radiopharmaceutical, and the adopted developing solvent is acetonitrile and ammonia water, and the volume ratio of the acetonitrile to the ammonia water is 90: 10-85: 15. According to the method, amino polyether and matrix components are separated by adopting a developing solvent with a specific volume ratio, and particularly, sodium ascorbate in the amino polyether and the matrix is separated.

The invention discloses a longan leaf granule which is prepared by the following steps: adding water into longan leaves, heating, extracting, filtering, concentrating, drying, crushing to obtain dry paste powder, adding a diluent into the dry paste powder, uniformly mixing, adding a wetting agent, granulating, and drying to obtain the longan leaf granule. The forming rate, the dissolution rate, the moisture absorption rate, the fluidity and the like of the granules all meet the requirements, and the granules are good in stability, suitable for industrial batch production, capable of treating diabetes, capable of meeting the requirements of doctors for treatment based on syndrome differentiation and increase and decrease along with symptoms and convenient to carry and take by patients. Thin-layer chromatography is adopted for qualitative identification of the longan leaf granules, high performance liquid chromatography is adopted for content determination of quercetin, quercitrin and kaempferol in the longan leaf granules, operation is easy, convenient and accurate, repeatability is good, and a scientific basis is provided for quality evaluation and control of the longan leaf granules.

The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to stasis-removing and pain-relieving pills and a quality detection method thereof. The stasis-removing and pain-relieving pill is prepared by taking radix paeoniae alba, rhizoma corydalis, radix paeoniae rubra, angelica sinensis, ligusticum wallichii, rhizoma cyperi, rhizoma zingiberis, fennel and astragalus membranaceus as raw materials and taking 10% starch slurry as an adhesive. The quality detection method of the stasis-removing and pain-relieving pill comprises general inspection, microscopic identification and thin-layer chromatography identification. And high performance liquid chromatography content determination. According to the present invention, by researching the preparation process and the quality standard of the stasis removing and pain relieving pill, the new thought and the new method are provided for the development of the traditional Chinese medicine preparation, the excellent choice is hopefully provided for the common gynecologic symptoms such as irregular menstruation, dysmenorrhea, postpartum blood stasis abdominal pain and the like, and the pain caused by the illness is relieved.

The invention belongs to the field of chemical analysis and testing, and particularly relates to an aminobenzaldehyde-based coumarin fluorescent probe for preparing a reagent for detecting mercaptosuccinic acid and a preparation method of the aminobenzaldehyde-based coumarin fluorescent probe. The preparation method comprises the following steps: dissolving 2-aminobenzaldehyde and 7-(diethylamino) coumarin-3-formic acid in dichloromethane, adding 1-ethyl-(3-dimethylamino aldehyde) carbodiimide hydrochloride (EDCI) and 1-hydroxybenzotriazole (HBOT) as condensing agents, carrying out a stirring reaction at room temperature, carrying out separation and purification on the obtained crude product through a thin-layer chromatography, and carrying out recrystallization to obtain the coumarin derivative. The final yellow product, namely the aminobenzaldehyde-based coumarin fluorescent probe Y-SH, is obtained. The coumarin fluorescent probe prepared by the preparation method disclosed by the invention can be used for rapidly detecting mercaptosuccinic acid in deionized water.

The present application belongs to the technical field of traditional Chinese medicine component detection, and particularly relates to a qualitative identification method of water-soluble components of safflower. The qualitative identification method of water-soluble components of safflower provided by the present application adopts water as an extraction solvent, extracts water-soluble components in safflower, and then adopts a thin layer chromatography method for identification, effectively solves the technical problem that safflower cannot be effectively detected after being boiled in water in the prior art, and is suitable for the inspection of classical famous prescription preparations containing safflower.

The invention belongs to the field of chemical analysis and testing, and particularly relates to a fluorescent probe for preparing a reagent for detecting elastase as well as a synthesis method and application of the fluorescent probe. The preparation method comprises the following steps: dissolving 2-dicyanomethylene-3-cyano-4, 5, 5-trimethyl-2, 5-dihydrofuran and 6-aminopyridine-3-formaldehyde in ethanol, and heating and refluxing a reaction solution at 50 DEG C under the acidic condition of ammonium acetate to obtain an intermediate TCF-N-NH2; and dissolving the intermediate TCF-N-NH2 and pentafluoropropionic anhydride in dichloromethane, stirring the reaction solution at the temperature of 0 DEG C to room temperature under the alkaline condition of pyridine, and separating and purifying the obtained crude product through thin layer chromatography to obtain the final orange product TCF-NF. The fluorescent probe obtained by the invention can quickly recognize elastase in deionized water.

The invention belongs to the technical field of medicines, and particularly relates to an infantile cough relieving syrup detection method which comprises the following steps: identifying radix peucedani, platycodon grandiflorum, pericarpium citri reticulatae and cortex mori radicis in infantile cough relieving syrup by adopting a thin layer chromatography; and simultaneously detecting the fructus arctii and the radix scutellariae by adopting a high performance liquid chromatography. The detection method for the infantile cough relieving syrup has the advantages of being rapid in analysis, easy to operate, high in accuracy, wide in adaptability and the like, and establishment and overall evaluation of the quality standard of the infantile cough relieving syrup are facilitated.

Bottom-Interface Thin Layer Chromatography Chamber

The utility model discloses an extrudable filtering beaker which comprises a beaker used for bearing silica gel fragments and serving as a sample pool; the sand filter plug is arranged in the beaker, and the edge of the sand filter plug is attached to the inner wall of the beaker; the bottom end of the push-pull rod is connected with the center of the sand filter plug, and the top end extends out of the beaker top opening. According to the utility model, the problem of inconvenience in filtration during preparation of the thin-layer chromatography can be solved.

The invention belongs to the technical field of quality detection of traditional Chinese medicines, and discloses a quality detection method of rhizoma atractylodis macrocephalae fried with fructus aurantii, which is characterized by comprising the following steps: performing qualitative analysis on rhizoma atractylodis macrocephalae fried with fructus aurantii by respectively adopting a thin-layer chromatography and an HPLC (High Performance Liquid Chromatography) characteristic chromatogram; a high performance liquid phase external standard curve method is adopted to carry out quantitative analysis on atractylenolide I, atractylenolide II, atractylenolide III, naringin and neohesperidin in the fructus aurantii fried bighead atractylodes rhizome. The invention establishes a thin-layer identification method for rhizoma atractylodis macrocephalae and fructus aurantii in a fructus aurantii fried rhizoma atractylodis macrocephalae processed product; according to the method for establishing the fingerprint spectrum, the similarity of 10 batches of rhizoma atractylodis macrocephalae fried with fructus aurantii ranges from 0.991 to 1.000; the method for determining the content of atractylenolide I, atractylenolide II, atractylenolide III, naringin and neohesperidin is established, scientific support is provided for formulating the quality standard of the characteristic processing decoction pieces of the temporary prescription, and effective guarantee is provided for clinical application of the decoction pieces.

This invention belongs to the field of traditional Chinese medicine detection technology, specifically relating to a multi-component thin-layer chromatography (TLC) identification method for pediatric runtong syrup. This invention establishes specific TLC identification processes for roasted radish seed, immature bitter orange, hemp seed, and scrophularia root, optimizing the sample pretreatment, developing solvent ratio, spotting volume, and colorimetric inspection conditions for each component. After specificity and robustness studies, the method demonstrates good stability under varying temperature, humidity, TLC plate type, and spotting volume ranges, exhibiting no negative interference and clear spots, enabling accurate multi-component identification. This invention is the first to establish a TLC identification method for these four medicinal materials in pediatric runtong syrup, providing a scientific and reliable technical means for the quality control of this compound preparation and filling a gap in existing technology.