138 results about "Thin-layer chromatography" patented technology

The invention discloses a radix astragali, rhizoma polygonati and ligustrum lucidum ait traditional Chinese medicine preparation for treating myelosuppression as well as a preparation method and a detection method thereof. The preparation is prepared from 50-150 parts of radix astragali, 50-150 parts of wine-processed rhizoma polygonati, 25-75 parts of wine-processed ligustrum lucidum ait, 50-150 parts of blanched rhizoma drynariae, 50-150 parts of radix trichosanthis and 25-75 parts of herba epimedii. The optimal extraction process and refining process are screened out based on effective components and pharmacological activity through a large number of tests, and the optimal preparation process of the Shuanghuangliangwhitening oral liquid and other traditional Chinese medicine preparations is obtained through stability investigation and verification. According to the invention, a thin layer chromatography is adopted, the four medicinal materials of astragalus membranaceus, glossy privet fruit, rhizoma drynariae and herba epimedii are respectively subjected to thin layer chromatography research, and methodology research shows that the method is stable and feasible. The invention establishes a content determination method for astragaloside, calycosin-7-glucoside, specnuezhenide and icariin, establishes a quality standard, and has important significance for ensuring the clinical curative effect and safety of astragaloside, calycosin-7-glucoside, specnuezhenide and icariin.

The invention relates to the technical field of pharmaceutical analysis, in particular to a thin-layer chromatography identification method of vitality-preserving decoction and a preparation thereof. Comprising the following steps: S1, extracting a sample to be detected by using diethyl ether, and preparing a test solution; s2, preparing a cinnamaldehyde reference substance solution and a 6-gingerol reference substance solution; the method further comprises at least one of the steps S3 and S4; s3, dripping the test solution and the cinnamaldehyde reference solution on the same thin-layer plate, developing with a first developing solvent, and identifying the cinnamon; the first developing solvent comprises petroleum ether and ethyl acetate in a volume ratio of (15-20): 3; s4, dripping the test solution and the 6-gingerol reference substance solution on the same thin-layer plate, developing with a second developing solvent, and identifying the ginger; the second developing solvent is prepared from petroleum ether, trichloromethane and ethyl acetate according to the volume ratio of (2-4): (1-2): (1-3). The method is good in overall separation effect, clear in spots, good in specificity and durability, and capable of identifying the medicine flavors of cinnamon and ginger in the vitality-preserving decoction and the preparation thereof.

The invention relates to the technical field of thin-layer chromatography detection, and discloses a thin-layer chromatography detection method for free fluorine-18 in a fluorine-18 labeled radiopharmaceutical, which adopts ethyl acetate and n-hexane as developing solvents, and the volume ratio of ethyl acetate to n-hexane is (50-90): (10-50). The developing solvent with a specific volume ratio is adopted, and the two key indexes that the Rf value of the marked active ingredient in the radiopharmaceutical is smaller than 0.8 and the separation degree (the separation degree of the marked active ingredient in the radiopharmaceutical and free fluorine-18) R is larger than 1 are considered.

The invention relates to a method for detecting a clearing agent main agent in gasoline, and the method comprises the following steps: carrying out thin-layer chromatography detection on a to-be-detected sample, and determining the composition of the clearing agent main agent in the concentrated to-be-detected sample according to color spot distribution on an obtained thin-layer chromatography plate, wherein the clearing agent main agent comprises one or more of a polyether amine clearing agent, a polyisobutene amine clearing agent and a Mannich base clearing agent; the thin-layer chromatography detection sequentially comprises developing and developing treatment; a developing agent for developing comprises one or more of a developing agent I, a developing agent II and a developing agent III; the developing solvent I is methanol, and the developing solvent II and the developing solvent III comprise one or more of alcohol with the carbon atom number of 1-6, aliphatic hydrocarbon with the carbon atom number of 5-12 and cycloalkane with the carbon atom number of 5-12. According to the method provided by the invention, whether the clearing agent main agent is added into the sample to be detected or not can be rapidly determined, and the type of the clearing agent main agent can be rapidly and accurately detected.

The present application provides a kind of Echinops formula granule quality detection method, including the thin layer identification of Echinops formula granule, characteristic map construction and β-ecdysterone content determination, the content standard of formula granule is limited to every 1g contains β-ecdysterone 3.5~14.4mg, thin layer identification is identified using thin layer chromatography, characteristic map construction and β-ecdysterone content determination are all using liquid chromatography method.The quality detection method of Echinops formula granule of the present application, by the detection of thin layer identification, characteristic map construction and β-ecdysterone content determination to assess the quality of Echinops formula granule, can comprehensively reflect the inherent quality of formula granule and better control the quality of Echinops formula granule.

The application discloses a kind of radionuclide 18 The present application discloses a method for detecting the radiochemical purity of F-labeled FAPI compounds, which comprises using thin layer chromatography to detect the radiochemical purity of F-labeled FAPI compounds 18 The present application discloses a method for detecting the radiochemical purity of F-labeled FAPI compounds, which comprises using thin layer chromatography to detect the radiochemical purity of F-labeled FAPI compounds 18 The present application discloses a method for detecting the radiochemical purity of F-labeled FAPI compounds, which comprises using thin layer chromatography to detect the radiochemical purity of F-labeled FAPI compounds 18 The present application discloses a method for detecting the radiochemical purity of F-labeled FAPI compounds, which comprises using thin layer chromatography to detect the radiochemical purity of F-labeled FAPI compounds 18 The present application discloses a method for detecting the radiochemical purity of F-labeled FAPI compounds, which comprises using thin layer chromatography to detect the radiochemical purity of F-labeled FAPI compounds 18 The present application discloses a method for detecting the radiochemical purity of F-labeled FAPI compounds, which comprises using thin layer chromatography to detect the radiochemical purity of F-labeled FAPI compounds 18 The present application discloses a method for detecting the radiochemical purity of F-labeled FAPI compounds, which comprises using thin layer chromatography to detect the radiochemical purity of F-labeled FAPI compounds 18 The present application discloses a method for detecting the radiochemical purity of F-labeled FAPI compounds, which comprises using thin layer chromatography to detect the radiochemical purity of F-labeled FAPI compounds 18 The present application discloses a method for detecting the radiochemical purity of F-labeled FAPI compounds, which comprises using thin layer chromatography to detect the radiochemical purity of F-labeled FAPI compounds 18 The present application discloses a method for detecting the radiochemical purity of F-labeled FAPI compounds, which comprises using thin layer chromatography to detect the radiochemical purity of F-labeled FAPI compounds 18 The present application discloses a method for detecting the radiochemical purity of F-labeled FAPI compounds, which comprises using thin layer chromatography to detect the radiochemical purity of F-labeled FAPI compounds

The invention relates to the technical field of thin-layer chromatography detection, and discloses a thin-layer chromatography separation method for amino polyether and a matrix in a fluorine-18 labeled radiopharmaceutical, and the adopted developing solvent is acetonitrile and ammonia water, and the volume ratio of the acetonitrile to the ammonia water is 90: 10-85: 15. According to the method, amino polyether and matrix components are separated by adopting a developing solvent with a specific volume ratio, and particularly, sodium ascorbate in the amino polyether and the matrix is separated.

The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to stasis-removing and pain-relieving pills and a quality detection method thereof. The stasis-removing and pain-relieving pill is prepared by taking radix paeoniae alba, rhizoma corydalis, radix paeoniae rubra, angelica sinensis, ligusticum wallichii, rhizoma cyperi, rhizoma zingiberis, fennel and astragalus membranaceus as raw materials and taking 10% starch slurry as an adhesive. The quality detection method of the stasis-removing and pain-relieving pill comprises general inspection, microscopic identification and thin-layer chromatography identification. And high performance liquid chromatography content determination. According to the present invention, by researching the preparation process and the quality standard of the stasis removing and pain relieving pill, the new thought and the new method are provided for the development of the traditional Chinese medicine preparation, the excellent choice is hopefully provided for the common gynecologic symptoms such as irregular menstruation, dysmenorrhea, postpartum blood stasis abdominal pain and the like, and the pain caused by the illness is relieved.

The present application belongs to the technical field of traditional Chinese medicine component detection, and particularly relates to a qualitative identification method of water-soluble components of safflower. The qualitative identification method of water-soluble components of safflower provided by the present application adopts water as an extraction solvent, extracts water-soluble components in safflower, and then adopts a thin layer chromatography method for identification, effectively solves the technical problem that safflower cannot be effectively detected after being boiled in water in the prior art, and is suitable for the inspection of classical famous prescription preparations containing safflower.

The invention belongs to the technical field of medicines, and particularly relates to an infantile cough relieving syrup detection method which comprises the following steps: identifying radix peucedani, platycodon grandiflorum, pericarpium citri reticulatae and cortex mori radicis in infantile cough relieving syrup by adopting a thin layer chromatography; and simultaneously detecting the fructus arctii and the radix scutellariae by adopting a high performance liquid chromatography. The detection method for the infantile cough relieving syrup has the advantages of being rapid in analysis, easy to operate, high in accuracy, wide in adaptability and the like, and establishment and overall evaluation of the quality standard of the infantile cough relieving syrup are facilitated.

This invention belongs to the field of traditional Chinese medicine detection technology, specifically relating to a multi-component thin-layer chromatography (TLC) identification method for pediatric runtong syrup. This invention establishes specific TLC identification processes for roasted radish seed, immature bitter orange, hemp seed, and scrophularia root, optimizing the sample pretreatment, developing solvent ratio, spotting volume, and colorimetric inspection conditions for each component. After specificity and robustness studies, the method demonstrates good stability under varying temperature, humidity, TLC plate type, and spotting volume ranges, exhibiting no negative interference and clear spots, enabling accurate multi-component identification. This invention is the first to establish a TLC identification method for these four medicinal materials in pediatric runtong syrup, providing a scientific and reliable technical means for the quality control of this compound preparation and filling a gap in existing technology.

The invention relates to the technical field of cistanche deserticola detection, in particular to a method for simultaneously and rapidly detecting four phenylethanoid glycosides in cistanche deserticola, which comprises the following steps: preparing a plurality of parts of mixed reference substance solutions containing four phenylethanoid glycosides with known concentrations; preparing a to-be-detected cistanche sample into a test solution; spotting the mixed reference substance solution and the test solution on the same thin-layer plate, carrying out thin-layer chromatography development by adopting a developing solvent, and volatilizing the solvent; placing the thin-layer plate after the solvent is volatilized under ultraviolet light to collect spot images, and processing the images by adopting image analysis software to obtain integral gray values of all phenylethanoid glycoside spots; respectively drawing a standard curve by taking the known concentrations of the four phenylethanoid glycosides in each mixed reference substance solution as horizontal coordinates and the corresponding integral gray values as vertical coordinates, establishing a linear regression equation, and calculating the contents of the four phenylethanoid glycosides in the cistanche deserticola sample to be detected; the invention provides a rapid and accurate method for determining the content of four phenylethanoid glycosides in cistanche.

The invention provides a triterpene enantiomer compound as well as a preparation method and application thereof, and relates to the technical field of medicines. The preparation method of the triterpenoid enantiomer compound comprises the following steps: (1) preparing a lycopodium clavatum extract: crushing a dried lycopodium clavatum whole plant, adding the crushed lycopodium clavatum whole plant into an ethanol solution for extraction, collecting an extracting solution, and concentrating the extracting solution under reduced pressure into paste to obtain a lycopodium clavatum crude extract; and (2) separation and purification: adding the crude lycopodium clavatum extract in the step (1) into deionized water for dilution, preparing a suspension, sequentially extracting with petroleum ether and chloroform, concentrating the chloroform extract into extract, and performing column chromatography, thin layer chromatography and molecular sieve chromatography separation to obtain the target triterpenoid enantiomer compounds named (+)-oncerin A (1) and (-)-oncerin A (2). The invention provides a new path for developing a new acetylcholin esterase inhibitor drug, and the application prospect is good.

The utility model discloses a light source adjusting device of a thin-layer chromatography scanner, and relates to the technical field of thin-layer chromatography scanners. The scanner comprises a scanner main body, a scanning table, a circuit board and a halogen tungsten lamp, the circuit board is installed inside the scanner main body, the halogen tungsten lamp is installed on a lamp holder of the circuit board, and a light source mechanism is arranged inside the scanner main body. The first gear drives the worm to rotate, the worm drives the worm gear to rotate, the transmission speed can be further reduced, the worm gear drives the transmission shaft to rotate, the transmission shaft drives the grating to rotate slowly and uniformly through the first rotating shaft, the situation that the grating is difficult to rotate uniformly through manual operation can be avoided, and the purpose of uniform-speed rotation is achieved. The threaded rod rotates to drive the moving block to move, the moving block drives the connecting block to move, and the connecting block drives the focusing lens to move, so that the position of the focusing lens can be adjusted to effectively focus light rays, and the purpose of improving the focusing effect is achieved.

The invention discloses a disubstituted halogenated azaanthrone visible light initiator, a preparation method and application of the disubstituted halogenated azaanthrone visible light initiator in an acrylate photopolymerization system. The method comprises the following steps: dissolving a 2, 4-disubstituted halogenated azacyclo-anthrone compound in an organic solvent under the protection of inert gas, adding excessive alkali, slowly dropwise adding excessive organic solution of an acyl chloride compound in an ice-water bath, reacting at room temperature, detecting that the reaction is complete by TLC (Thin Layer Chromatography), stopping the reaction, performing suction filtration, concentrating filtrate, and drying to obtain the 2, 4-disubstituted halogenated azacyclo-anthrone compound. And purifying to obtain the novel disubstituted halogenated azacyclo-anthrone high-efficiency visible light initiator. The novel disubstituted halogenated azacyclo-anthrone high-efficiency visible light initiator not only shows better solubility in an organic solvent and resin, but also shows higher polymerization efficiency in a photocuring system, so that the application range of the novel disubstituted halogenated azacyclo-anthrone high-efficiency visible light initiator in a free radical photocuring system is widened.

The invention relates to the technical field of quality control of traditional Chinese medicines, in particular to a thin-layer chromatography identification method for 8 medicines in 11 medicines in the whole formula of Qingjin phlegm-reducing decoction. The core of the method is that three different test solutions are adopted to realize the identification of eight medicines, namely scutellaria baicalensis, rhizoma anemarrhenae, exocarpium citri rubrum, fructus gardeniae, liquorice, thunberg fritillary bulb, platycodon grandiflorum and radix ophiopogonis. Wherein the first test solution is prepared by extracting water-saturated n-butyl alcohol, and can be simultaneously used for identifying scutellaria baicalensis, rhizoma anemarrhenae, exocarpium citri rubrum, fructus gardeniae and liquorice; the second solution is subjected to ammoniation and dichloromethane extraction to be used for thunberg fritillary bulb identification; the third solution is extracted by dichloromethane after being hydrolyzed by hydrochloric acid, and platycodon grandiflorum and radix ophiopogonis can be identified at the same time. According to the method, by optimizing a test sample preparation and development system, sharing of test samples of multiple medicines and synchronous identification are achieved, the detection efficiency is remarkably improved, the method has the advantages of being easy and convenient to operate, clear in spot, good in separation degree, high in specificity, negative, free of interference and the like, and a reliable basis is provided for quality control of the Qingjin phlegm reducing decoction.

The application discloses a detection method for separating diglyceride in vegetable oil and qualitative analysis of the diglyceride, and the diglyceride in the vegetable oil is dissolved by using n-hexane to obtain a sample for standby; different proportion eluent is used to wash and elute a solid phase extraction column, and eluent is collected and dried for standby to obtain eluate; the eluate is analyzed and quantified by using a thin layer chromatography and gas chromatography, and finally, the eluate is finely qualitatively analyzed by using a triple quadrupole; the separation method is simple, the separation of the diglyceride in the vegetable oil can be realized by using two extraction cartridges, the test steps are simplified, the separation purity is high, the cost and time are reduced; finally, qualitative analysis is performed by using GC-MS, the analysis time is short, the specific fatty acid composition in the diglyceride is more finely analyzed, and the qualitative analysis detection of food nutrition and the guidance of related food production are facilitated.

The invention discloses a rapid detection method of isobutyramido thiazolyl resorcinol in cosmetics, and belongs to the technical field of safety detection and analysis of cosmetics. Thin-layer chromatography is taken as a main line, separation, 254nm and 365nm dual-band ultraviolet observation, 105 DEG C thermal activation enhancement and three-channel color development recheck of ferric trichloride, vanillin-sulfuric acid and phosphotungstic acid are sequentially completed, and the detection result is accurate. The method comprises the following steps: acquiring multilayer evidences of physical separation, optical response and chemical reaction, establishing a standardized ratio shift value according to upper and lower interior labels and a solvent front edge, extracting indexes such as brightness and color difference, dispersing displacement and intensity signals into five-bit bar codes, realizing fault-tolerant matching by using a Hamming distance, outputting positive, confirmation or negative conclusions, and determining the accuracy of the result. And generating a report containing the original image and the parameters. The method is oriented to complex matrixes such as emulsion, face cream, essence, mask liquid, lipstick and eye shadow, by means of multi-mechanism cross validation and bar code type fusion interpretation, the false positive and false negative risks are remarkably reduced, and the result consistency and the rechecking efficiency are improved.

The application discloses a method for detecting clenbuterol based on thin layer chromatography and surface enhanced resonance Raman spectroscopy, and belongs to the technical field of food detection. The method comprises the following steps: (1) adopting thin layer chromatography to preliminarily separate the clenbuterol component from the matrix in the pig liver sample, and confirming the clenbuterol component in the pig liver sample under the ultraviolet lamp of 254 nm; (2) adding aromatic diazonium salt solution to the same Rf value of the clenbuterol component reference sample spot to generate azo compounds, and then adding a surface enhancer silver sol; (3) in-situ detecting the surface enhanced resonance Raman spectrum of the corresponding azo compound component spot, and if the surface enhanced resonance Raman spectrum of the pig liver sample solution at the same Rf value is the same as that of the reference sample solution, it is determined that the pig liver sample contains the clenbuterol component. The application has high specificity for detecting clenbuterol and salbutamol, high sensitivity, fast analysis speed, and is beneficial to fast detection of grass-roots units. The application is suitable for detecting clenbuterol.

The invention provides a method for identifying and detecting a phlegm stasis and arthralgia dredging prescription. The method comprises three modules of construction of a characteristic chromatogram of a medicinal material and optional thin-layer chromatography identification and effective component content determination. The construction of the characteristic spectrum adopts a diethyl ether-ethyl acetate two-stage segmented extraction UPLC analysis strategy, so that the interference of complex components is overcome, and the overall chemical characteristics of the compound can be comprehensively represented; thin-layer chromatography identification provides a specific identification method for medicines such as coptis chinensis, curcuma wenyujin and vinegar-processed rhizoma corydalis; and the content determination adopts a high performance liquid chromatography to detect the effective components chlorogenic acid, salvianolic acid B, glycyrrhizic acid and berberine hydrochloride. According to the invention, a multi-dimensional and configurable quality control system is constructed, the comprehensive quality control of the phlegm stasis and arthralgia dredging prescription from qualitative and overall characterization to quantitative analysis is realized, the specificity is strong, and the result is reliable.

The invention provides a quality standard of a rungia pectinata medicinal material and a detection method thereof, and relates to the technical field of traditional Chinese medicines. The invention provides a quality standard detection method for Rungia pectinata medicinal materials, which detects the quality of Rungia pectinata from the aspects of medicinal material sources, character identification, microscopic identification, thin-layer identification, moisture, total ash content, acid-insoluble ash content and extracts, and establishes a thin-layer chromatography identification method with strong specificity, stability and reliability. Therefore, the authenticity of the rungia pectinata medicinal material is identified, the quality of the rungia pectinata medicinal material is evaluated, and the medication safety is ensured. According to the quality standard of the Rungia pectinata medicinal material established by the method, the identification result is stable and reliable, and the accuracy is high.

The utility model provides a kind of thin layer chromatogram sample application device, including base, the base is provided with sample application platform, the sample application platform is provided with sample application seat, the one side of the sample application platform is provided with support, the support is provided with sample application head;The base is provided with intermittent drive assembly, the intermittent drive assembly is matched with the sample application platform;The inside of base is provided with support frame, the rotating motor passes through the support frame and is connected with the drive shaft, the drive shaft is connected with the drive gear, one end of the transmission shaft is connected with the support frame, and the other end passes through the transmission gear and is connected with the driving element, the transmission gear is engaged with the drive gear.The thin layer chromatogram sample application device provided by the utility model is provided with intermittent drive assembly, the intermittent rotation of sample application platform is driven by gear engagement driving element, continuous sample application can be realized, and sample application efficiency is improved.

The invention discloses nucleus eliminating granules which are prepared by the following steps: adding water into 12 medicinal materials, namely selfheal, oyster, kelp, beautiful sweetgum fruit, fructus aurantii, cowherb seed, salvia miltiorrhiza, curcuma zedoary, ligusticum wallichii, white paeony root, corydalis tuber and radix bupleuri, heating, extracting, filtering, concentrating and drying to obtain medicinal powder; and sieving, adding auxiliary materials, adding a wetting agent, preparing a soft material, granulating, and drying to obtain the kernel eliminating granules. The invention further discloses a preparation method of the nucleus eliminating particles, a thin-layer chromatography identification and content determination method and application of the nucleus eliminating particles. According to the invention, the nucleus-eliminating mixture is developed into the nucleus-eliminating particles, the forming rate, the dissolution rate, the moisture absorption rate, the fluidity and the like of the nucleus-eliminating particles all meet the requirements of Chinese Pharmacopoeia, and the stability experiment result shows that the product stability is good; the curative effect of the nucleus eliminating granules on breast pain, breast nodules, hyperplasia of mammary glands and breast diseases can be achieved; doctors realize accurate medication under treatment based on syndrome differentiation, and the flexibility of adjusting prescriptions along with symptoms is guaranteed; on the other hand, more convenient carrying and taking experience can be provided for the patient.

The present invention relates to the technical field of drug analysis, and specifically relates to an analytical method for Litsea lancilimba formula granules. The Litsea lancilimba formula granules are formed by decocting Litsea lancilimba decoction pieces in water while collecting volatile oil and carrying out concentration, drying, and granulation. The analytical method comprises the following items: descriptions, identification, tests, extractives, characteristic chromatographic fingerprints, and assay. The identification method uses thin-layer chromatography for identification; in the characteristic chromatographic fingerprints, by taking the peak corresponding to the reference peak of magnoflorine reference substance as an S peak, high performance liquid chromatography is used to mark four characteristic peaks; and the assay is to determine the content of magnoflorine. The analytical method established in the present invention improves the analytical requirements for medicinal materials, decoction pieces, standard decoctions and formula granules of Litsea lancilimba, which helps to establish a perfect quality control system for various products of Litsea lancilimba, and helps to ensure the quality and clinical efficacy of the Litsea lancilimba formula granules.

The application provides a kind of stone lotus seed extract, bitter stone lotus extract thin layer identification method, comprising: taking stone lotus seed extract pretreatment, to obtain test solution;Take bitter stone lotus extract pretreatment, to obtain bitter stone lotus extract solution;Take stone lotus control medicinal material, and water decoction is obtained control medicinal material solution;Test solution, bitter stone lotus extract solution and control medicinal material solution are detected by thin layer chromatography, and thin layer plate is silica gel G thin layer plate;Developing agent is n-butanol-isopropyl alcohol-water-glacial acetic acid;Under the observation of ultraviolet light, if test solution and control medicinal material solution have the same color fluorescent spot at Rf equal place, while there is no fluorescent spot at Rf=0.64-0.79, it is stone lotus seed extract;If there is fluorescent spot at Rf=0.64-0.79, it is bitter stone lotus extract.The application is used for distinguishing stone lotus seed extract and bitter stone lotus extract by whether there is fluorescent spot in the range of relative migration value in thin layer chromatogram.

The invention belongs to the field of traditional Chinese medicine extracts, and relates to a triterpenoid alkaloid compound as well as a preparation method and application thereof. The triterpenoid alkaloid compound is separated from euonymus japonicus for the first time, and it is proved that the triterpenoid alkaloid compound has remarkable anti-inflammatory activity and anti-tumor activity and has good application prospects in preparation of anti-inflammatory drugs and anti-tumor drugs. The preparation method comprises the following steps: taking dry euonymus japonicus as a raw material, sequentially carrying out heating reflux extraction for multiple times by using methanol, extracting an alkaloid-rich part by using an acid extraction and alkali precipitation method, carrying out preliminary segmentation by means of normal-phase silica gel column, amino silica gel column, gel column chromatography and the like, and carrying out refining purification by a preparative thin-layer chromatography, a semi-preparative high performance liquid chromatograph and the like. The preparation method is easily available in raw materials, environment-friendly, simple to operate and suitable for industrial production.

The present application relates to the technical fields of medical and pharmaceutical chemical synthesis, and particularly relates to a synthesis method of 3-thiocyanatospiro cyclobutyl oxindole compounds, which comprises the following steps: in a reactor, compound 1, compound 2, a catalyst, an additive and a solvent are stirred for 30 minutes at room temperature, after the reaction is completed, the crude product is obtained by reduced pressure distillation, and the 3-thiocyanatospiro cyclobutyl oxindole compound is obtained by column chromatography and thin layer chromatography purification. N The present application takes phenyl bicyclo[1.1.0]butane-1-formamide compounds and thiocyanate as raw materials, and promotes the synthesis of 3-thiocyanatospiro cyclobutyl oxindole compounds by an oxidant. The present application has the advantages of simple synthesis steps, mild conditions, safe operation, good functional group compatibility, high reaction conversion, low cost and the like, and is conducive to industrial production.

The invention discloses a thin-layer chromatography identification method and application of radix cynanchi auriculati traditional Chinese medicine formula granules and radix cynanchi auriculati traditional Chinese medicine formula granules as well as a preparation method of the radix cynanchi auriculati traditional Chinese medicine formula granules and the radix cynanchi auriculati traditional Chinese medicine formula granules, and relates to the technical field of detection methods. The method comprises the following steps: dripping a test solution on a silica gel G thin-layer plate, and developing and inspecting under thin-layer chromatography conditions; thin-layer chromatography conditions are as follows: n-hexane-ethyl formate-formic acid is used as a developing solvent; the volume ratio of the normal hexane to the ethyl formate to the formic acid is 20: 15: 1; the spreading temperature is 4-26 DEG C, and the spreading humidity is 30% rh to 60% rh; compared with a thin-layer chromatogram of the radix cynanchi auriculati traditional Chinese medicine formula granules, the radix cynanchi auriculati charcoal traditional Chinese medicine formula granules have fluorescent spots at the specific shift value of 0.421-0.458. The method can be used for rapidly and effectively identifying the radix cynanchi bungei traditional Chinese medicine formula granules or radix cynanchi bungei charcoal traditional Chinese medicine formula granules which lose the form of the decoction pieces, is simple and convenient to operate, high in precision and sensitivity and good in stability, and has a good application prospect.

The invention discloses a synthesis method of diaryl methyl sulfide under a biological compatibility condition, which comprises the following steps: adding a p-methylene benzoquinone derivative and a thiophenol thiol derivative into a reaction solvent, stirring and reacting for 4 hours at room temperature to obtain a reaction solution, filtering and removing the reaction solvent of the reaction solution to obtain diaryl methyl sulfide. And purifying by thin layer chromatography / column chromatography to obtain the diaryl methyl thioether compound. The p-methylene benzoquinone derivative used in the method is easy to synthesize, high in conversion rate and wide in applicable substrate range, and in addition, the method is simple in step, convenient to operate and high in product yield, does not use any metal compound, and has the advantages of being high in atom economy and environmentally friendly.

The invention relates to the technical field of general trace detection, in particular to a TLC (thin-layer chromatography) detection method for pharmaceutical ingredients of QAMS (quantitative analysis of multi-components by single marker) in heat-clearing and detoxifying granules, which comprises the following steps: performing thin-layer chromatography detection on a plurality of to-be-detected solutions prepared from to-be-detected drugs; performing closed graph detection on the target image to obtain a graph area; performing a first matching operation on the graphic area and a corresponding historical area in the historical image to obtain a first trailing value; performing second matching operation on the target graph area and the corresponding target historical area to obtain a second trailing value; and determining a detection optimization strategy for thin-layer chromatography detection according to a trailing classification result corresponding to the second trailing value of the target graphic area, and obtaining a single-detection multi-evaluation result of the to-be-detected liquid based on the detection optimization strategy. According to the invention, the operation speed and the accuracy of the TLC detection method for the medicinal components for quantitative analysis of multi-components by single marker in the heat-clearing and detoxifying granules are improved.