20 results about "Toad skin" patented technology

This invention belongs to the field of traditional Chinese medicine, specifically relating to a topical delay spray and its preparation method. The invention uses the dried epidermis of the Huaxi toad (with the toad venom removed), and extracts it efficiently through a combination of water and alcohol extraction. Male silkworm moth oil can nourish and strengthen the body, tonify the liver and kidneys, and combat fatigue; studies have shown that male silkworm moth oil can effectively treat kidney yang deficiency. This invention combines the synergistic effects of toad skin extract and male silkworm moth oil to prepare a delay spray that can significantly treat premature ejaculation and erectile dysfunction in men. This preparation has the advantages of direct action, sustained release, avoidance of drug contact, and simple operation. This invention further improves the rational utilization system of toad resources, provides a practical solution for the efficient development of toad medicinal resources, and significantly reduces production and clinical application costs.

The invention discloses a traditional Chinese medicine composition for breaking blood and removing blood stasis and a blood stasis breaking pill. The traditional Chinese medicine composition comprises the following raw material medicines in parts by weight: 5-7 parts of centipede, 2-4 parts of scorpion, 0.5-1.5 parts of toad skin, 8-12 parts of pseudo-ginseng, 5-7 parts of gecko, 2-4 parts of myrrh, 0.5-1.5 parts of safflower, 0.5-1.5 parts of ligusticum wallichii and 5-7 parts of pig nail. Animal drugs including centipede, scorpion, gecko and toad skin are main drugs and belong to insect drugs, traditional Chinese medicine believes that the properties of the animal drugs are good, and the animal drugs can combat poison with poison and break blood to remove blood stasis; pseudo-ginseng, safflower, ligusticum wallichii and myrrh are classic medicines for promoting blood circulation to remove blood stasis, so that the effects of activating meridians to stop pain are enhanced, and the drasticality of insect medicines is alleviated.

The present invention belongs to the field of biotechnology, and specifically relates to a differential characteristic polypeptide library for identifying different medicinal parts of Chinese giant toads and its application. In the prior art, there are differences in protein components between toad venom and toad skin, toad meat, and toad bones, but the detection of protein macromolecules is relatively cumbersome, which is not conducive to the conversion of methods into standards. The present invention uses polypeptides as index components to perform identification and analysis on toad venom and dried toad medicinal materials, which has strong specificity, good stability, is not disturbed by the processing process, and can identify multiple medicinal materials at the same time, with obvious advantages.

The scheme discloses an alkaloid compound with 14, 21 oxygen bridge structures and a preparation method and application thereof in the field of traditional Chinese medicine extraction and separation. The ethyl acetate extraction part of ethanol extracts of toad skin is repeatedly separated and purified by combining various chromatography technologies; 1D and 2D nuclear magnetic and high resolution mass spectrometry are used to identify the structures of two new compounds, and the molecular formula and structure formula of the two new compounds are deduced. In vitro pharmacodynamic studies show that the two alkaloid compounds with 14, 21 oxygen bridge structures have good inhibition on colon cancer cells (HCT-116), and IC 50 The two alkaloid compounds with 14, 21 oxygen bridge structures have good inhibition on colon cancer cells (HCT-116), and IC 50 values are 0.92 μM and 10.91 μM respectively. The present application is helpful for the development and utilization of natural compounds from toad.

The invention discloses a traditional Chinese medicine composition for treating tumors caused by internal stagnation of toxic blood stasis. The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 47-49 parts of longpimpinella pedicularis, 24-26 parts of black ants and 24-26 parts of toad skin. According to the invention, only the three medicinal materials, namely Longdiland, black ant and toad skin, are adopted, the formula is simple, the medicinal materials are wide in source and controllable in cost, the pharmacological actions of the three medicinal materials are complementary, Longdiland enhances immunity and resists inflammation, black ant regulates immunity, resists inflammation and relieves pain, toad skin directly plays an anti-tumor role, the effects of attacking hard mass, breaking stasis, resisting cancer and diminishing swelling are realized, and a precise formula is prepared aiming at the pathogenesis of internal stagnation of toxic blood stasis; clinical verifications prove that the traditional Chinese medicine can effectively relieve clinical symptoms of tumors caused by internal accumulation of toxic stasis and control recurrence and metastasis of the tumors; meanwhile, the body immunity of patients is improved, the life quality is improved, the survival time is prolonged, and compared with existing partial traditional Chinese medicine preparations, the curative effect is clearer, and the pertinence is higher. The invention further discloses a preparation method of the traditional Chinese medicine composition.

The invention discloses preparation and application of toad skin heparinoid sulfate capable of inhibiting activity of new spike protein. The preparation method comprises the following steps: crushing dried toad skin, heating in a water bath to swell, carrying out enzymolysis by using alkaline protease, and carrying out alcohol precipitation, anion exchange column purification, dialysis and freeze drying to obtain a toad skin glycosaminoglycan crude extract; crude toad skin glycosaminoglycan extracts are subjected to enzymolysis through chondroitin sulfate ABC enzyme, and the toad skin heparinoid sulfate is obtained through gel chromatographic column purification, desalination and freeze drying. The average molecular weight of the toad skin heparinoid sulfate is 11Kda, the toad skin heparinoid sulfate is composed of disaccharide units formed by connecting hexuronic acid and glucosamine through 1-4 glucosidic bonds, and the total sulfation degree of the C-2-oxygen site of hexuronic acid and the total sulfation degree of the C-6-oxygen site and the total sulfation degree of the N-site of glucosamine are 11.45%, 33.68% and 46.46% respectively. The toad skin heparinoid sulfate has competitive activity of inhibiting binding of new crown spike protein and heparin, and can prevent initial infection of new crown virus. The invention lays an important foundation for the development of new clinical application of the toad skin.

The invention relates to the technical field of artificial breeding of amphibians, in particular to an ecdysis promoting method for artificially imitating wild breeding of toads and application, according to the ecdysis promoting method, the toads are soaked in a soaking solution containing iodophor, the effective iodine concentration in the soaking solution is 0.1-0.2%, and the soaking time is 10-25 minutes. By the adoption of the ecdysis promoting method, the ecdysis period is greatly shortened, and compared with natural ecdysis (12-15 days), the ecdysis period is shortened to 5-7 days, and efficiency is greatly improved; and the toad cortex quality and yield can be obviously improved, and the toad cortex integrity reaches 92% or above.

The application discloses a kind of polypeptide, polypeptide extraction and purification method with river toad as raw material and application thereof, belong to the field of bioactive peptide, its amino acid sequence is FPGGPGAK, and preparation method and application in the field of cosmetics are also disclosed.The polypeptide disclosed in the application, polypeptide extraction and purification method with river toad as raw material and application thereof are extracted, purified and screened by treating river toad skin, and the central peptide segment playing a photoprotection role is used in cosmetics.

The application belongs to the field of traditional Chinese medicine, and particularly relates to an external use delayed oil agent and a preparation method thereof. The dried skin of the Visternotus limnocharis which has scraped toad venom is combined with the male silkworm moth which has the functions of tonifying liver and kidney, strengthening yang and astringing essence to significantly improve male premature ejaculation and erectile dysfunction through water extraction and alcohol extraction. The male silkworm moth oil has unique promotion potential and development potential. The application combines the toad skin and the male silkworm moth oil to prepare the delayed oil agent, significantly improves male premature ejaculation and erectile dysfunction, and has the characteristics of direct action and simple operation. The preparation cost is low.

The invention relates to a medicated thread for treating nasopharynx cancer, a preparation method and a treatment method, and belongs to the field of medical treatment, the medicated thread is prepared by fusing toad skin small molecule peptide extracted by soaking with acetic acid, iron ions provided by medicinal ferrous sulfate and iodine glycerin. The treatment method comprises the following steps: precisely embedding the medicated thread into a nasopharyngeal tumor under the guidance of a nasal endoscope, and combining microwave treatment after 3-5 days. The synergistic effect of the Fenton reaction initiated by iron ions and the tumor anti-oxidation system inhibited by the toad skin small molecule peptide effectively induces ferroptosis of tumor cells, and the treatment effect is enhanced by combining the heat effect of microwaves. According to the invention, minimally invasive targeted therapy is realized, anti-tumor immunity of a body can be activated, and a new treatment strategy is provided for nasopharynx cancer.

This invention discloses a compound preparation of toad skin and its preparation method, which is made by processing dried toad skin, astragalus, and tortoise shell powder in a certain proportion. Compared with the prior art, this invention has the effects of invigorating qi and tonifying the kidney, detoxifying and relieving pain. The formulation of this invention is more reasonable, and it has significant curative effects on mid-to-late stage tumors, chronic hepatitis B and other diseases, without any toxic side effects.

Provided are an iodine-131-labeled small molecule polypeptide TCMP-Y1, a preparation method therefor, and a use thereof. TCMP-Y1 is a small-molecule polypeptide isolated and identified from toad skin secretions in the early stage and has an amino acid sequence of YFKAWGWPIDDATTEHL-NH2. The polypeptide TCMP-Y1 has no obvious proliferation inhibitory effect on prostate cancer cells. However, iodine-131-labeled 131I-TCMP-Y1 can bind to prostate cancer cells and is stably retained within the prostate cancer cells, and has the inhibitory effects on tumor cell proliferation and tumor growth of the prostate cancer cells both in vivo and in vitro. In addition, the water-soluble 131I-TCMP-Y1is mainly excreted by means of the renal-urinary system, thereby reducing the risk of radiation-induced liver injury. The TCMP-Y1 is a small-molecule polypeptide having excellent nuclide-carrying capability, improves drug safety and tumor targeting, and can be used as a potential drug for internal irradiation therapy for tumors.

The invention discloses a formula for treating tumors and a preparation process, relates to the technical field of medicaments, and researches and develops a medicine which is safe and effective, can play a better role in treating middle and advanced stage tumors and has a treatment effect on related diseases. The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 10 to 30 parts of dried toad skin, 15 to 35 parts of radix fici simplicissimae, 5 to 20 parts of radix astragali seu hedysari essence, 3 to 15 parts of radix ginseng, 1 to 5 parts of in-vitro cultured calculus bovis, 0.1 to 1 part of artificial musk, 5 to 15 parts of vinegar frankincense and 5 to 15 parts of vinegar myrrh. The formula disclosed by the invention has the effects of detoxifying, diminishing swelling and relieving pain. The traditional Chinese medicine is used for treating middle and advanced tumors, chronic hepatitis B and the like; and the traditional Chinese medicine composition can be used for treating ulcer, furunculosis, scrofula, abscess and cancerous swelling caused by heat toxin accumulation.

The invention discloses a traditional Chinese medicine composition for treating nasopharynx cancer and a preparation method of a nano freeze-dried tablet of the traditional Chinese medicine composition. The traditional Chinese medicine composition is characterized by being prepared from the following raw materials in parts by weight: 25-35 parts of astragalus membranaceus, 25-35 parts of ganoderma lucidum spore powder, 25-35 parts of centipeda minima, 25-35 parts of cortex juglandis mandshuricae, 8-12 parts of dried toad skin, 2-4 parts of centipede, 1-3 parts of gecko, 10-20 parts of ligusticum wallichii, 10-20 parts of sculellaria barbata, 25-35 parts of oldenlandia diffusa, 10-20 parts of paris polyphylla, 5-10 parts of liquorice, 8-12 parts of magnolia flower and 5-10 parts of fresh ginger. The nasopharynx cancer can be treated through the traditional Chinese medicine, so that the defects of operative treatment, radiotherapy and chemical treatment are overcome, and the treatment effect is improved.

The invention discloses an anti-uterine cancer traditional Chinese medicine targeted multilayer tablet and a preparation method thereof, and is characterized in that the anti-uterine cancer traditional Chinese medicine targeted multilayer tablet is prepared from the following components by weight: 150-250g of edible tulip, 75-90g of toad skin, 150-200g of centipede, 80-120g of astragalus membranaceus, 100-150g of fructus viticis, 50-80g of ganoderma lucidum spore powder, 50-60g of sculellaria barbata, 100-150g of semen coicis, 20-40g of liquorice and 60-100g of oldenlandia diffusa. The medicine effect can be improved, and growth, metastasis and diffusion of tumors can be effectively inhibited.

The present invention belongs to the technical field of traditional Chinese veterinary medicine, and provides a traditional Chinese medicine compound preparation for preventing and controlling swine respiratory diseases, a preparation method thereof and an application. The traditional Chinese medicine compound preparation comprises scutellaria baicalensis, medicated leaven, pinellia ternata, peach kernel, forsythia suspensa, toad skin, Chinese gall, honeysuckle flower, bupleurum chinense, cordyceps militaris, inula japonica, verbena officinalis, dandelion, and liquorice. The traditional Chinese medicine compound preparation provided by the present invention has the biological functions of protecting the integrity of the respiratory system mucosa and improving the disease resistance of the body, effectively reducing the conversion rate of mild or common diseased pigs, not producing any toxic and side effects after use, having no drug resistance, and having a simple formula, convenient use, good curative effect, high protection and cure rates.

The invention discloses a traditional Chinese medicine compound multi-layer coated microsphere preparation for treating pancreatic cancer and a preparation method thereof. The preparation is prepared from the following active components in parts by weight: 10-35 parts of astragalus extract, 8-25 parts of ganoderma lucidum spore powder, 5-18 parts of sculellaria barbata general flavone and the like. The preparation method comprises the following steps: a) directionally extracting active ingredients; b) removing toxic substances from the dried toad skin through molecular imprinting; c) constructing three layers of coated microspheres, wherein a core layer is a nanocrystal (indirubin / chelidonine / toad peptide), a middle layer is a phospholipid complex (flavone / polysaccharide), and an outer layer is a pH response polysaccharide microsphere; and d) pressing to obtain the three-layer sustained-release tablet. The preparation realizes colon-targeted drug release, the bioavailability is obviously improved, the tumor inhibition rate of animal experiments is improved, and the chemotherapy drug resistance can be reversed.

The invention relates to a dried toad skin charcoal hemostatic traditional Chinese medicine as well as a preparation method and application thereof. The preparation method comprises the following steps: grinding dried toad skin, and calcining the ground dried toad skin in a muffle furnace to obtain toad skin charcoal powder; adding distilled water of which the weight is 8-10 times that of the toad skin charcoal powder into the toad skin charcoal powder, decocting for 15-25 minutes, pouring out filtrate, adding distilled water of which the weight is the same as that of the toad skin charcoal powder again, decocting for 15-25 minutes, and concentrating the filtrate obtained by two times of decoction until the weight is 3-5 times that of the toad skin charcoal powder; s2, the concentrated solution obtained in the step S2 is filtered through a Buchner funnel and a cellulose acetate membrane in sequence and then dialyzed through a 1000 Dalton dialysis membrane for 70-75 h, the dialyzed solution is subjected to centrifugal separation to obtain a toad skin carbon dot (CB-CDs) solution, and the CB-CDs solution is freeze-dried to obtain the dried toad skin carbon hemostasis traditional Chinese medicine. The obtained dried toad skin charcoal hemostatic traditional Chinese medicine has good performance.

The application belongs to the field of traditional Chinese medicine, and particularly relates to an external use delayed time towel agent and a preparation method thereof. The dried skin of the Chinese toad from which the toad venom has been scraped is selected, water extraction and alcohol extraction are combined, toad skin extract is efficiently extracted, and the toad skin extract is combined with male moth oil to prepare the delayed time towel agent. It is found that through the synergistic effect of the toad skin extract and the male moth oil, male premature ejaculation and erectile dysfunction can be significantly improved, and the synergistic effect of the two is much higher than that of single medicine, the medicinal effect is optimized, the core requirement of clinical application is met, the towel agent is prepared, independent packaging is achieved, and the towel agent has the characteristics of low cost, direct effect, simple operation, and difficulty in drug pollution.