21 results about "Heparinoid" patented technology

The utility model relates to the technical field of heparin processing, in particular to heparinoid processing and purifying equipment, which comprises an ion exchange resin cylinder and a purifying cylinder, a filter screen frame is mounted at the upper end of the ion exchange resin cylinder through a mounting rack, ion exchange resin is arranged in the ion exchange resin cylinder, and the purifying cylinder is mounted on the filter screen frame. A purification cylinder is arranged at one end of the ion exchange resin cylinder, a top cover is clamped at the top of the purification cylinder, a glass observation opening is embedded in one side of the purification cylinder, an electric heater is mounted on one side of the bottom end of the glass observation opening through a mounting hole, and a temperature controller is mounted at the upper end of the electric heater through a screw; a through guide sleeve is arranged at one end of the top of the top cover, and a metal material taking straight pipe is arranged in the through guide sleeve in a sleeved mode. According to the heparinoid processing and purifying equipment, the supernate and the heparinoid-containing precipitate can be conveniently and rapidly separated and subjected to evaporation and dehydration treatment, water and organic solvents in the supernate and the heparinoid-containing precipitate are removed, the processing efficiency is improved, and manpower and material resources are saved.

The present invention is directed to hexasaccharides mimetics of heparins presenting remarkable heparanase inhibition activity. The compounds have formula (I), wherein R1 is selected from the group consisting of NH2, NHSO3Na, NHAc; R2 is selected from the group consisting of NH2, NHSO3Na, NHAc; R3 is selected from the group consisting of Bn, H; R4 is selected from the group consisting of Ac, H, SO3Na; R5 is CO2Na; R6 is selected from the group consisting of Me, Ethyl, Alkyl, alkyl azide, alkynyl, cholestanol aglycon; R7 is selected from the group consisting of CH2OH, COOH.

Provided is a method by which a heparin-like substance having higher activity can be produced. The present invention provides a method for producing a heparin-like substance, the method comprising the steps of: (1) preparing a mammalian cell that produces a heparin-like substance; and (2) culturing the mammalian cell prepared in the step (1), which produces the heparin-like substance, at a temperature effective for suppressing cell proliferation, and causing the mammalian cell to produce the heparin-like substance. The temperature effective for suppressing proliferation is preferably a temperature which is 2 to 14°C lower than the optimum temperature for the proliferation.

To provide a skin care preparation effective for improving skin elasticity and / or improving flabby skin.SOLUTION: The heparinoid is used as an active ingredient of a skin care preparation for external use effective for improving skin elasticity and / or improving sagging skin.SELECTED DRAWING: None

This invention discloses a qualitative and quantitative analysis method for heparin-like components in the biopharmaceutical field. The method includes using a liquid chromatograph, an SAX strong anion exchange column, sodium dihydrogen phosphate solution as mobile phase A, and mobile phase B consisting of mobile phase A containing sodium perchlorate, with gradient elution for monitoring and analysis. This invention improves the separation of heparin-like components by screening chromatographic packing materials, optimizing the chromatographic method, and optimizing the detection wavelength. It allows for accurate qualitative analysis of up to five heparin components using a simple liquid chromatography method. Furthermore, this invention incorporates the simulated separation function of the Glycomapping glycosylation software to reconstruct the elution peaks of components that do not reach baseline separation, and then achieves quantitative analysis of each separated component based on the external standard method. This qualitative and quantitative analysis method provides a feasible solution for component identification and content determination in heparin-like samples, and offers an important means for heparin quality control.

The application discloses a kind of white snail glycosaminoglycan-growth factor composite active substance directional extraction purification process, belong to biological active substance extraction technical field.Core breakthrough is in at: through-10 ℃ acetone circulation degreasing and 20kHz ultrasonic coordination papain-neutral protease compound system (mass ratio 1:0.8), realize glycosaminoglycan and growth factor synchronous efficient release, extraction rate reaches 97.2%;Using DEAE-Sepharose Fast Flow and Q Sepharose Fast Flow double column chromatography gradient elution, combined with 1000Da ultrafiltration membrane refining, composite active substance purity is ≥99.6%;Through-Vc mild depolymerization technology regulates glycosaminoglycan molecular weight to 3.8~23.8kDa, retains its heparin-like structure activity;Using porous medium enhanced vacuum freeze-drying process, so that composite active substance activity retention rate is ≥98.5%.Product glycosaminoglycan content is ≥35mg / g, growth factor (EGF class) activity is ≥1200IU / mg, DPPH free radical clearance rate is ≥65%, to skin fibroblast proliferation promotion rate is ≥72%, no organic solvent residue and heavy metal pollution.

A carbonated aerosol external preparation for skin, including a stock solution containing the following components (A) to (C), and a propellant containing the following component (D): (A) heparinoid, (B) water-soluble polymer, (C) water, and (D) carbon dioxide gas. The carbonated aerosol external preparation for skin has a pH immediately after discharge of 4.0 or more and 7.0 or less. The component (B) includes one or more selected from the group consisting of hydroxypropylmethylcellulose and acrylic acid / alkyl (meth)acrylate copolymer. A content of the component (B) in the stock solution is from 0.05 to 5.0% by mass, based on a total mass of the stock solution.

To provide a topical pharmaceutical composition containing indomethacin, diclofenac sodium, felbinac, salicylic acids, or heparins, wherein irritation to the application site is reduced. [Solution] A topical pharmaceutical composition that contains component (A) and component (B) below, wherein component (A) is one or more selected from the group consisting of components (A-1) to (A-5) below, and which reduces irritation to the application site. Ingredient (A-1): Indomethacin Ingredient (A-2): Diclofenac sodium Ingredient (A-3): Felbinac Ingredients (A-4): Salicylic acids Ingredient (A-5): Heparinoid Ingredient (B): One or more selected from the group consisting of loxoprofen, its salts, and their hydrates.

This invention discloses a heparin-like hydrogel microsphere for melanoma postoperative wound repair, its preparation method, and its application, belonging to the field of medical dressing technology. The preparation of the heparin-like hydrogel microsphere for melanoma postoperative wound repair includes the following steps: dissolving KOSMA and GelMA in water to prepare a GelMA solution; adding a photoinitiator to form a precursor hydrogel solution; using the precursor hydrogel solution as the dispersed phase and castor oil as the continuous phase to prepare hydrogel microspheres; collecting the effluent; exposing it to ultraviolet light for curing; washing; and drying to obtain the final product. When used for melanoma postoperative wound care, these hydrogel microspheres can safely promote wound healing, specifically eliminate residual tumor cells, reduce the postoperative local recurrence rate, and can also serve as drug carriers to achieve synergistic therapeutic effects; providing new research ideas and technical strategies for integrated care of melanoma postoperative wounds and tumor prevention and control.

The application discloses an enhanced anti-coagulation and anti-bacterial hemodialysis hollow fiber membrane and a preparation method thereof, and belongs to the technical field of medical membrane materials. The acid activation-polydopamine coating-sulfonated grafting modification of halloysite nanotubes is carried out in the application, so that the surface sulfonic acid group density of the halloysite nanotubes reaches 0.8-2.1 mmol / g, and the Zeta potential is less than or equal to-30 mV. The modified halloysite is dispersed in a polar aprotic solvent in advance, is incorporated into a polymer matrix after ultrasonic treatment, is filtered through a sintering filter element with a diameter of less than or equal to 5 mu m, is defoamed, is spun by using a dry spraying-wet spinning method, and is prepared into a finished product through coagulation, rinsing and drying. The application constructs a heparin-like negative charge layer, so that the activated partial thromboplastin time (APTT) is greater than or equal to 55 s, platelet adhesion and protein adsorption are significantly inhibited, and the application has a broad-spectrum antibacterial performance. The rigid halloysite induces the formation of through channels, so that the pain point that a high-flux membrane is easy to damage red blood cells is solved, the process is stable, and the application is suitable for industrial production.

The object of this disclosure is to provide a topical composition comprising ceramide 2 and isopropyl myristate that can suppress creaming. [Solution] A topical composition containing (A) ceramide 2, (B) isopropyl myristate, and (C) heparinoid has suppressed creaming.

The invention relates to the technical field of biological pharmacy, in particular to a method for extracting heparinoid from heparin sodium alcohol precipitation alcohol, which comprises the following steps: S1, pretreatment: filtering the collected heparin sodium alcohol precipitation alcohol to obtain clear filtrate; s2, concentration: concentrating the clarified filtrate in a reduced pressure distillation manner, and stopping distillation when the clarified filtrate is concentrated to 1 / 5-1 / 3 of the original volume to obtain a concentrated solution; s3, precipitation: slowly adding acetone into the concentrated solution while stirring, and precipitating and separating out the heparinoid to obtain a solid-liquid mixture. According to the method, a large amount of waste alcohol precipitation alcohol generated in the heparin sodium production process is deeply developed, the heparinoid product with the high additional value is extracted, waste of heparinoid resources is greatly reduced, and the economic benefits of enterprises are improved; meanwhile, organic substances in the waste liquid are recycled, so that the chemical oxygen demand of the discharged waste liquid is effectively reduced, the subsequent sewage treatment cost is reduced, and good environmental benefits are achieved.

The application belongs to the technical field of medicine and cosmetics, and relates to a kind of ultra-low molecular weight heparin fusion molecules and its preparation method and application.The structure is as shown in formula I, wherein x is greater than or equal to 0, y is greater than or equal to 1;R1 and R2 are each independently selected from -SO3Na or -H;R3 is;Weight average molecular weight is 3-4kDa.The structure of the ultra-low molecular weight heparin fusion molecule provided by the application contains hydrophobic amide thiazole resorcinol structure and hydrophilic ultra-low molecular weight heparin structure at the same time, which are combined by covalent ester bond, has higher safety and excellent water solubility, and improves the problems of poor water solubility of amide thiazole resorcinol and safety risk of heparin products used in cosmetics.

This invention provides a natural rubber anticoagulant and latex-expelling accelerator and its application. The accelerator includes an anticoagulant, a cut repair agent, and an active stabilizer. The anticoagulant is a citrate, heparin ammonium salt, or dithiothreon derivative; the cut repair agent is a phenylurea derivative; and the active stabilizer is at least one of sorbitol, phenacetin, polyvinylpyrrolidone, and sodium octaborate tetrahydrate. The accelerator of this invention is safer and more efficient when applied to rubber tree tapping, promoting latex excretion and tree recovery, effectively saving bark consumption, significantly reducing ethylene stimulation concentration, effectively reducing the side effects of ethylene, obtaining ideal yield, accelerating the repair of cut bark, and reducing the risk of pathogen infection of wounds.

The present application relates to sewage biological technology field, disclose a kind of resource processing method of activated sludge.The resource processing method of activated sludge includes the following steps: washing activated sludge obtains sediment sludge;Sediment sludge is mixed with water to obtain suspension, suspension is digested, and first supernatant and sludge residue are obtained by separation;First supernatant is impurity-removed by calcium salt solution and alcohol solvent to obtain first precipitate;First precipitate is dissolved and separated to obtain second supernatant;Second supernatant is impurity-removed by alcohol solvent to obtain second precipitate;Second precipitate is dissolved and separated to obtain third precipitate;Third precipitate is dissolved and separated to obtain third supernatant, and third supernatant is impurity-removed by alcohol solvent to obtain fourth precipitate;Fourth precipitate is purified to obtain heparinoid polysaccharide.This method has good impurity-removing effect, high polysaccharide yield, and the sludge residue obtained by separation can be used for fermentation to produce methane, and the heparinoid polysaccharide obtained has similar blood clotting function and anticancer potential to commercially available heparin, and has high application value.

Provided is a chemically modified bovine intestinal beparin, as well as pharmaceutical compositions, compos itions comprising chemically modified bovine intestinal heparin, and methods for making and using the same.

An aerosol carbonic acid skin external preparation, which is composed of an aerosol base liquid containing components (A) to (C) and a propellant containing component (D), and has a pH value of 4.0 or more and 7.0 or less immediately after being sprayed. (A) heparinoid, (B) water-soluble polymer, (C) water, (D) carbon dioxide.

Heparin compounds and synthetic heparin analogues having short acting anticoagulant activity are provided. Methods of synthesizing such heparin compounds, including chemoenzymatic pathways using sulfotransferase enzymes are provided. Methods of treating subjects in need of anticoagulant activity are provided.

The present invention is directed to synthetic hexasaccharide mimics of heparin having formula (I) wherein: R1 is selected from the group consisting of NH2, NHSO3 -, NHAc; R2 is selected from the group consisting of H, SO3 -, Bn, 2-Naphthylmethyl, preferably H; R3 is selected from the group consisting of H, Ac, SO3 -; R4 is CO2 -; R5 is Me, Et, C3-C30 linear or branched alkyl, optionally comprising one or more isolated or fused rings, PEG-OMe; R6 is selected from the group consisting of H, Ac, SO3 -; R is a C10-C30 linear or branched alkyl optionally comprising one or more isolated or fused rings. In another embodiment, the invention is directed to the use as a drug of hexasaccharides having the above formula, wherein R, R1, R2, R3, R4 and R6 have the above defined meaning, while R5 is selected from the group consisting of Me, Et, C3-C30 linear or branched alkyl, optionally comprising isolated or fused rings, PEG-OMe.

The invention relates to the technical field of biological medicine, and discloses a method for improving gene deletion virus preparation efficiency, which comprises the following steps: (1) constructing a transfer plasmid and a knockout plasmid (sgRNA) according to a virus target gene; (2) adding a heparinoid metal complex and cholate into a growth culture medium to prepare a high-permeability cell culture solution for cell transfection; (3) adding the high-permeability culture medium into a cell culture dish, adding plasmids embedded by lipidosome, incubating, and inoculating; and (4) purifying to obtain the constructed gene deletion virus. According to the method for improving the preparation efficiency of the gene deletion virus, a metal compound is directionally brought to the surface of a cell through heparinoid, the transfection efficiency is improved only by changing the permeability and fluidity of a cell membrane, the permeability of liposome is not changed, the efficiency of embedding exogenous DNA by the liposome is not influenced, and the transfection efficiency is improved; therefore, the success rate of gene deletion virus construction is improved.

In a composition combining (A) pyridoxine salts, etc. and (B) heparinoids, the photostability of (A) pyridoxine salts, etc. is improved while maintaining the effect of improving the skin barrier function. [Solution] (A) One or more selected from the group consisting of pyridoxine, its derivatives and pharmaceutically acceptable salts thereof, (B) Heparin-like substances, and (C) Nicotinamide, A topical skin preparation composition containing [a specific ingredient].