Low-molecular-weight heparin affinity purification medium and method for purifying low-molecular-weight heparin

Abstract

The invention provides a low-molecular-weight heparin affinity purification medium, an affinity purification polyethylene imine dextran microsphere formed by the medium and a low-molecular-weight heparin affinity purification method, belonging to the field of biological medicine. The low-molecular-weight heparin affinity purification medium comprises three components: polyethylene imine, cross-linking substances and a microsphere structure, wherein the cross-linking substances comprise a cross-linking agent and a surfactant; the microsphere structure comprises microspheres and a microsphere carrier. The low-molecular-weight heparin can be well collected and other types of heparins are also removed when the low-molecular-weight heparin is purified by the purification medium; the purpose of well separating and purifying is achieved; the low-molecular-weight heparin can be used for quickly and efficiently preparing low-molecular-weight heparin sodium in a large scale.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to a low molecular weight heparin affinity purification medium, an affinity purification polyethyleneimine dextran microsphere formed from the medium and a low molecular weight heparin affinity purification method. Background technique [0002] Heparin sodium is the sodium salt of glucosamine sulfate extracted from the intestinal mucosa of pigs, which belongs to mucopolysaccharides. Heparin is a linear chain molecule composed of hexasaccharide or octasaccharide repeating units. Disaccharide trisulfate unit is the main disaccharide unit of heparin, L-iduronic acid is the uronic acid of this disaccharide, the uronic acid of disulfate disaccharide is D-glucuronic acid, disaccharide trisulfate and disaccharide Sulfated disaccharides are linked alternately in a ratio of about 3:1. Heparin sodium white powder, hygroscopic, stable to heat. Soluble in water, insoluble in organic solvents such as ...

AI Technical Summary

Problems solved by technology

The chemical method to produce low molecular weight heparin mainly uses chemical reagents such as sodium nitrite and hydrogen peroxide to degrade heparin. The chemical method is randomly degraded, has no selectivity, and has poor specificity. The activity of Factor Xa decreases, resulting in by-products that have no anticoagulant effect. Therefore, it is necessary to efficiently purify low-molecular-weight heparin to improve potency and reduce the risk of side effects

[0005] At present, the purification of low-molecular-weight heparin sodium is mainly based on the oxidation method, which is obtained by fractional precipitation with organic solvents. This method has the following defects: 1. The oxidizing agent is difficult to remove; 2. The structure of low-molecular-weight heparin sodium is easy to destroy

Therefore, the production of large-scale rapid and efficient preparation of low molecular weight heparin sodium is limited.