6results about How to "Efficient separation and purification" patented technology

The invention belongs to the technical field of nucleic acid extraction, and particularly relates to a method for separating and extracting purified nucleic acid from different cell components. The invention develops a separation and purification method suitable for liquid-phase RNA (Ribonucleic Acid) of a cytoplasm component, a nuclear free component and the like, so that the extracted RNA can be compatible with the highest standard of nucleic acid mass spectrum and high-throughput sequencing, and the operation flow is simpler, the time is shorter, the cost is lower, and the extraction effect is better and more efficient; meanwhile, a corresponding nucleoplasm DNA / RNA separation buffer is developed on the basis, and an efficient nucleic acid purification process is matched, so that the genome DNA can be completely extracted with high quality and high purity while high-quality chromatin RNA is obtained, and another level of information, namely genome DNA information, is provided for a classical component separation scheme of RNA dynamic biology; therefore, all nucleic acid information of each component of the cell can be efficiently separated and purified.

The utility model discloses a garlic extract liquid separation purification device, including raw material tank, pH regulation mechanism, garlic polysaccharide extraction mechanism and alliinase extraction mechanism, the pH regulation mechanism includes first pH regulation device, centrifuge and second pH regulation device that are connected through pipeline in proper order, the first pH regulation device is connected with raw material tank through pipeline, the centrifuge is connected with the second pH regulation device through pipeline, and the alliinase extraction mechanism is connected with the second pH regulation device through pipeline. The garlic polysaccharide extraction mechanism comprises a resin column, an ultrafiltration concentration device, a water purification device and a dialysis device, an ultrafiltration permeate outlet of the ultrafiltration concentration device is sequentially connected with the water purification device and the dialysis device through pipelines, and a concentrated solution outlet of the ultrafiltration concentration device is connected with the dialysis device through a pipeline; a liquid outlet of the dialysis device is sequentially connected with an airflow dryer and a garlic polysaccharide product tank through pipelines, and the allinase extraction mechanism comprises a dissolving device, an ultrafiltration device and an allinase product tank which are sequentially connected through pipelines. The garlic polysaccharide and alliinase separation and purification device is simple in process, good in impurity removal effect, capable of effectively separating and purifying garlic polysaccharide and alliinase and high in product purity.

This utility model discloses a pretreatment device for raw materials in the production of high-purity Kathon, relating to the field of Kathon production technology. The inlet of an ultrasonic reactor is connected via pipelines to a methylchloroisothiazolinone raw material tank and a deionized water tank. The outlet of the ultrasonic reactor is connected via a screw pump to a ceramic membrane device. The liquid phase outlet of the ceramic membrane device is connected via pipelines to a distillation column. The top gas phase outlet of the distillation column is connected via pipelines to a light component tank, an organic solvent tank, and a purified methylchloroisothiazolinone tank. This device achieves effective separation and purification of different components, improving product purity, while simultaneously recovering organic solvents and reducing production costs.

The invention discloses a preparation method and application of an iPS (induced pluripotent stem) exosome, and relates to the technical field of biologication.The preparation method comprises the steps of overexpression vector construction, nanoparticle preparation and transfection, iPS cell optimized culture, exosome separation, exosome purification, BMSC (bone mesenchymal stem cells) and exosome co-culture and bone defect repair experiment. The method has the advantages that the overexpression vector containing genes related to osteocyte differentiation, such as RUNX2 and ABC1, is constructed through gene editing, the vector is accurately introduced into the iPS cells by virtue of unique nano particle mediated transfection and magnetic field guidance, the transfection efficiency is greatly improved, the oxygen concentration is dynamically adjusted according to time in a culture link, and the osteocyte differentiation effect is improved. Meanwhile, substances such as a serum substitute and the like are added into the culture medium, a suitable growth environment is created for the iPS cells, the iPS cells are promoted to secrete high-quality exosomes, and the iPS cells are efficiently separated and purified by applying an advanced microfluidic technology and an affinity chromatography method, so that the yield and the quality of the exosomes are greatly improved.

The invention belongs to the technical field of functional materials, and discloses a functional temperature-sensitive surfactant for HRP (horse radish peroxidase) in selective froth flotation crude extract as well as a preparation method and application of the functional temperature-sensitive surfactant. A hydrophilic temperature-sensitive monomer acrylamide AAm, a hydrophobic monomer acrylonitrile AN and a monomer glycidyl methacrylate GMA with an epoxy group are used as polymeric monomers, RAFT polymerization is performed to obtain the temperature-sensitive surfactant P (AAm-co-AN-co-GMA), the surface tension is regulated and controlled by regulating and controlling the length and temperature of a hydrophobic chain segment, and the temperature-sensitive surface active agent P (AAm-co-AN-co-GMA) is obtained. The performance and the switchable foam stability regulation effect are tested. Based on the excellent foam stabilizing performance of the temperature-sensitive surfactant and the high specific binding capacity of the aptamer to a target enzyme, the aptamer is modified to the temperature-sensitive surfactant, and the selectively functionalized temperature-sensitive surfactant P (AAm-co-AN-co-GMA)-Apt is prepared. HRP in a crude extract prepared from fresh horseradish is taken as a research target, and high-affinity binding and selective froth flotation of P (AAm-co-AN-co-GMA)-Apt to HRP are realized.

The invention provides a method for separating and purifying ascomycin. The method for separating and purifying ascomycin comprises the following steps: 1) adding inorganic salt into fermentation liquor containing ascomycin, adjusting the pH value of a system to be 1-4, and carrying out first ceramic membrane filtration to obtain first ceramic membrane concentrated liquor; adding alkaline buffer salt into the first ceramic membrane concentrated solution, adjusting the pH value of the system to 8-11, and carrying out second ceramic membrane filtration to obtain a second ceramic membrane concentrated solution; (2) carrying out filter pressing and water washing on the second ceramic membrane concentrated solution to obtain a filter cake, and extracting the filter cake by using an organic solvent to obtain an extracting solution; (3) cooling, crystallizing, filtering and drying the extract liquor to obtain first purified crystallis.By means of the method, cross-linked impurities such as amino acid, protein and pigment in the fermentation liquor can be effectively removed, and the effect of efficiently separating and purifying high-purity ascomycin is achieved.