Preparation and purification method for heparitin sulfate disaccharide, and purified product thereof

A technology of heparin sulfate and purification method is applied in the field of preparation and purification of natural products, which can solve the problems of high price, inability to prepare disaccharide structure, and numerous preparation steps, and achieve the effect of simple purification method.

CN102199175AInactive Publication Date: 2011-09-28FUZHOU UNIV

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Patent Information

Authority / Receiving Office: CN · China
Patent Type: Applications(China)
Current Assignee / Owner: FUZHOU UNIV
Publication Date: 2011-09-28
Estimated Expiration: Not applicable · inactive patent

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Abstract

The invention relates to a preparation and purification method for heparitin sulfate disaccharide, and a purified product thereof. The preparation and purification method comprises the following steps: eluting heparin disaccharide used as a raw material with a cation exchange resin, and regulating the pH value of the eluate to be weak acidic with pyridine to obtain heparin pyridine disaccharide; separately enabling the heparin pyridine disaccharide to react with a 1-methyl-2-pyrrolidone aqueous solution and an acetic anhydride, Na2CO3 and sulfur trioxide trimethylamine complex; and separating the reaction liquid with a gel resin, and purifying to obtain free amino disaccharide, N-acetyl disaccharide and N-sulfated disaccharide. The preparation and purification method is simple, solves the problems that a specific disaccharide structure can not be prepared in large scale, the preparation process comprises too many steps and the product price is expensive, and simultaneously further develops the study on the correlation between the special structure and function of heparitin sulfate.

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Description

technical field

[0001] The invention belongs to the field of preparation and purification of natural products, and more specifically relates to a preparation and purification method of heparan sulfate disaccharide and its purified product. Background technique

[0002] Heparan sulfate is composed of three regions with different degrees of sulfation alternately: the N-acetylated region (NA) is composed of completely unsulfated GlcNAc-GlcUA disaccharide units; the N-sulfated region (NS) is composed of Highly sulfated GlcNS(±6S)-IdoUA(2S) disaccharide composition, each disaccharide has 2-3 sulfate groups; and N-acetylation / N-sulfation region (NA / NS) is a transition region, Contains both sulfur-free and sulfur-containing disaccharides and crossed arrangements, the sequence is roughly GlcNAc-GlcUA-GlcNS-IdoA. The 12 representative disaccharide structures present in heparan sulfate are as follows figure 1 shown, which contains GlcNAc, GlcNSO 3 The disaccharide of the residue is...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine

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