193results about "Aminosugars" patented technology

The invention relates to the technical field of biology, and particularly discloses a method for removing lactulose-based isomers in an oligosaccharide separation and purification process. The method comprises the following steps: taking oligosaccharide fermentation liquor for producing oligosaccharide through fermentation by using lactose as a substrate, sequentially carrying out thallus removal, decoloration, electrodialysis desalination, ion exchange resin desalination, concentration, sequential simulated moving bed chromatographic purification, ultrafiltration, re-concentration and spray drying, and detecting the oligosaccharide and the lactulose-based isomer in the oligosaccharide. According to the method disclosed by the invention, the content of lactulose-based isomers in the separation and purification process is greatly reduced, so that the product purity is effectively improved; the method can be applied to the synthesis process of other milk oligosaccharides such as fucose lactose (FL), lactose-N-tetraose (LNT), lactose-N-neotetraose (LNnT), lactose-N-fucopentasaccharide (LNPFI) and the like or other carbohydrates containing a lactosyl group, has a very high application value, and accords with laws and regulations.

The invention relates to the technical field of oxidation resistance, in particular to a glutamic acid derivative, a preparation method thereof and application of the glutamic acid derivative in rhythm gene regulation and oxidation resistance. The glutamic acid derivative has a structure as shown in a formula I. The glutamic acid derivative provided by the invention can up-regulate expression of CLOCK protein and Per3 protein, can inhibit ROS in cells and up-regulate expression of Nrf2, and can be applied to relieving skin problems caused by disorder of circadian rhythm. # imgabs0 #

The invention relates to polystyrene cation exchange resin as well as a preparation method and application thereof. The preparation method comprises the following steps: dissolving a dispersing agent in a solvent to obtain a dispersed phase for suspension polymerization reaction, uniformly mixing a reaction monomer, a cross-linking agent, a pore-foaming agent and an initiator, and carrying out suspension polymerization reaction under segmented stirring and segmented temperature change to prepare copolymer microspheres; and putting the dried microspheres into a reaction kettle, swelling, carrying out sulfonation reaction through a variable temperature program on the basis to obtain sulfonated microspheres, and carrying out alkali exchange reaction on the sulfonated microspheres to obtain the final product cation exchange resin. Compared with the prior art, the cation exchange resin preparation method provided by the invention has the characteristics of simple and feasible process, easiness in large-scale production, low cost, high specific surface area, high adsorption capacity and high separation efficiency, and is especially suitable for separating and purifying substances containing amino groups or aminoglycosidic bonds.

Compounds and compositions that have an asialoglycoprotein receptor (ASGPR) binding ligand bound to an extracellular protein binding ligand for the selective degradation of the target extracellular protein in vivo to treat disorders mediated by the extracellular protein are described.

Compounds and compositions that have an asialoglycoprotein receptor (ASGPR) binding ligand bound to an extracellular protein binding ligand for the selective degradation of the target extracellular protein in vivo to treat disorders mediated by the extracellular protein are described.

A method and application of Escherichia coli BL21star (DE3) BLBRTXST3 and its fermentation method for producing high-purity lactose-N-neotetraose, the invention relates to the technical field of human milk oligosaccharide production, Escherichia coli )BL21 star (DE3) BLBRTXST3 is deposited in the General Microbiology Center of China Microorganism Culture Collection Committee, with the deposit number CGMCC No. 32521 and the deposit date November 6, 2024. The address of the deposit institution is No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing; Escherichia coli ( Escherichia coli )BL21 star (DE3) BLBRTXST3 is used in the fermentation production of lactose-N-neotetraose (LNnT). The present invention improves the yield of lactose-N-neotetraose, uses a chromatographic simulated moving bed to produce high-purity lactose-N-neotetraose with a purity greater than 94%, replaces the traditional solvent crystallization method, has simple separation and purification, is conducive to industrial production, and simultaneously produces linear lactose-N-neohexaose with a purity greater than 95%.

Extracellular protein degraders and compositions are provided that have a potent asialoglycoprotein receptor (ASGPR) Binding Ligand bound to an Extracellular Protein Targeting Ligand for the selective degradation of the Target Extracellular Protein, for example immunoglobulin in vivo to treat disorders mediated by the extracellular protein.

Compounds and compositions that have an asialoglycoprotein receptor (ASGPR) binding ligand bound to an extracellular protein binding ligand for the selective degradation of the target extracellular protein in vivo to treat disorders mediated by the extracellular protein are described.

Compounds and compositions that have an asialoglycoprotein receptor (ASGPR) binding ligand bound to an extracellular protein binding ligand for the selective degradation of the target extracellular protein in vivo to treat disorders mediated by the extracellular protein are described.

The invention belongs to the technical field of pesticides, and particularly relates to azobenzene calix [4] arene derivatives as well as a preparation method and application thereof. The invention discloses a novel azobenzene calix [4] arene derivative prepared by directional modification through an azo reduction reaction based on a calix [4] arene skeleton. The azobenzene calix [4] arene derivative has the characteristics of green environmental protection, accurate and controllable structure and efficient synthesis. After the derivative and a pesticide form a host-guest compound, the activity of the pesticide can be remarkably improved, the bioavailability of the pesticide at an action site is increased, the problem of low efficiency of the traditional pesticide is effectively solved, and the development of target drug resistance is delayed. The technology breaks through the technical bottleneck of nano-carrier environmental toxicity, provides a new thought for developing a multi-target and low-environmental-risk pesticide synergist, and has important significance for realizing pesticide decrement, synergism and resistance treatment.

The invention relates to the technical field of medicinal chemistry, and provides a TLR4 agonist, application thereof and a vaccine composition. The TLR4 agonist is a compound with a structure as shown in a formula 1 or a pharmaceutically acceptable salt thereof, the compound is glucosamine aminoalkyl 4-phosphate series molecules derived from MPL-12 structure modification, and the series molecules have enhanced immunostimulatory activity and selective immune response induction capability.

Described herein are bifunctional compounds for removing macrophage migration inhibitory factor (MIF) or immunoglobulin G (IgG). Further described herein are pharmaceutical compositions comprising these bifunctional compounds. Further described herein are methods for treating pathologies and / or conditions with said compounds or compositions. The pathologies and / or conditions are mediated through MIF / IgG or where MIF / IgG is a contributing factor in the development and perpetuation of diseases and / or conditions, such as autoimmune diseases and cancers, among others. TIFF2025509736000504.tif99145

The invention relates to the technical field of pesticide preparation processing, in particular to supramolecular amino-oligosaccharide, a nano preparation containing the supramolecular amino-oligosaccharide and a preparation method and application of the supramolecular amino-oligosaccharide. The supramolecular amino-oligosaccharide is provided, and the supramolecular amino-oligosaccharide is obtained by combining amino-oligosaccharide with water molecules or hydrogen peroxide molecules through acid; wherein amino groups in the amino-oligosaccharide and carboxyl groups of acid can be subjected to an acid-base reaction to form chemical bonds such as amido bonds, meanwhile, water molecules or hydrogen peroxide molecules participate in the reaction process, hydrogen atoms in the water molecules or the hydrogen peroxide molecules and atoms such as nitrogen and oxygen in the amino-oligosaccharide or acid molecules can form hydrogen bonds, and the structure of the molecules can be stabilized; therefore, the overall charge state of the formed supramolecular amino-oligosaccharide is more stable, it is ensured that the amino-oligosaccharide is stable and not prone to being decomposed in the compounding process, the pesticide effect of the supramolecular amino-oligosaccharide can be effectively exerted, and application is promoted.

A polymeric material comprising a base monomer and a multifunctional biobased molecule wherein the multifunctional biobased molecule is a glucose derivative. A polymeric material comprising a base polymer and a polymeric additive comprising glucaric acid, glucodialdose, gluconic acid, erythorbic acid, 2-ketoglucose, lactic acid, adipic acid, sorbitol, glycerol, mannitol, lactones thereof, salts thereof, or combinations thereof wherein the additive when introduced to a base polymer forms a modified polymer wherein the modified polymer has a tensile strength that is increased by from about 10% to about 100% wherein compared to the base polymer.

The present invention relates to a microorganism having an enhanced activity of a protein according to any one of the following [1] to [4] and improved productivity of an oligosaccharide having a Lewis X skeleton as compared with a parent strain: [1] a protein consisting of the amino acid sequence represented by SEQ ID NO: 2, [2] a mutant protein having an α1,3-fucosyl transferase activity and consisting of an amino acid sequence in which 1 to 20 amino acids are deleted, substituted, inserted, or added in the amino acid sequence represented by SEQ ID NO: 2, [3] a homologous protein having an α1,3-fucosyl transferase activity and consisting of an amino acid sequence having an identity of 90% or more with the amino acid sequence represented by SEQ ID NO: 2, and [4] a protein consisting of the amino acid sequence represented by SEQ ID NO: 24, 18, or 26.

The invention relates to the technical field of functional food, medicine and health care, and particularly discloses a chitosan oligosaccharide unsaturated fatty acid salt derivative and a preparation method and application thereof.The chitosan oligosaccharide unsaturated fatty acid salt derivative is a compound shown in the formula (1), and the preparation method comprises the specific preparation steps that chitosan oligosaccharide and unsaturated fatty acid are subjected to an ionic reaction, and the chitosan oligosaccharide unsaturated fatty acid salt derivative is obtained. The chitosan oligosaccharide unsaturated fatty acid salt derivative shown in the formula (1) is prepared, and the molar ratio of chitosan oligosaccharide to unsaturated fatty acid is 1: 1-1: 3. According to the derivative, chitosan oligosaccharide with excellent biocompatibility is used as a framework, and an unsaturated fatty acid group is combined to 2-position amino positive ions of the chitosan oligosaccharide in a zwitterion combination mode, so that the chitosan oligosaccharide unsaturated fatty acid salt derivative is prepared. The derivative has the characteristics of two compounds of chitosan oligosaccharide and unsaturated fatty acid at the same time, and can remarkably inhibit the activity of alpha-glucosidase, so that the effect of assisting in reducing blood sugar is achieved.

The invention discloses an anti-tumor composition containing N-acetyl-D-glucosamine and a preparation method of the anti-tumor composition in the field of anti-tumor preparations. The anti-tumor composition comprises the following components in parts by weight: 1-3 parts of functionalized N-acetyl-D-glucosamine and 3-5 parts of a polypeptide modifier. Through polypeptide modification and application of the N-acetyl-D-glucosamine, the N-acetyl-D-glucosamine (GlcNAc) as a bioactive sugar molecule can possibly have the following effects in tumor microenvironment regulation and control through the immune regulation function of the N-acetyl-D-glucosamine; glycometabolism interference: cancer cells usually grow rapidly depending on the Warburg effect, and the functionalized N-acetyl-D-glucosamine can inhibit proliferation of the cancer cells by influencing the glycometabolism of the cancer cells, and can synergistically play an anti-tumor role in three directions of glycometabolism interference, immunoregulation and cell apoptosis induction.

To provide a method for quantifying chitosan and a method for preparing a sample used for quantitative analysis of chitosan by high performance liquid chromatography, which enable accurate quantification of chitosan content in food through simple operation.SOLUTION: A method for quantifying chitosan comprises a dissolution step in which chitosan contained in a food is dissolved in an acidic aqueous solution, an enzymatic degradation step of enzymatically degrading the chitosan using chitosanase, an acid degradation step of degrading the chitosan into glucosamine using an acid, and a quantification step of quantifying the glucosamine by high performance liquid chromatography to quantify the chitosan content.SELECTED DRAWING: None

The present invention relates to the production of sialylated Human Milk Oligosaccharides (HMOs), in particular to the production of sialyl-lacto-N-neotetraose (LST-c), from precursor oligosaccharides and the genetic engineering of suitable cells for use in said production, as well as to methods for producing said sialylated HMOs.

The application belongs to the technical field of biological medicine, and particularly relates to a galactosamine-doxetaxel conjugate as well as a preparation method and application thereof. First, doxetaxel and 3,3'-diseleno-dipropionic acid are used as raw materials, and after heating reaction, 3,3'-diseleno-dipropionic acid doxetaxel is obtained. Then, the obtained 3,3'-diseleno-dipropionic acid doxetaxel and galactosamine are used as raw materials, and after heating reaction, galactosamine-3,3'-diseleno-dipropionic acid doxetaxel conjugate is obtained through column chromatography separation and purification. The obtained conjugate can be specifically recognized by endogenous lectin receptors (such as asialoglycoprotein receptor ASGPR) which are highly expressed on the surface of hepatoma cells, so as to be taken up by hepatoma cells through a receptor-mediated pathway, and has application prospect in the direction of hepatoma targeted treatment.

The invention relates to a novel chemical cross-linking agent with mass spectrum fragmentable trehalose disaccharide as a skeleton structure and a preparation method thereof. The cross-linking agent disclosed by the invention has the following characteristics: 1) trehalose disaccharide is used as a skeleton structure, so that the cross-linking agent has excellent biocompatibility; 2) the trehalose skeleton has a pair of symmetrical mass spectrum fragmentable glucosidic bonds, so that a cross-linked peptide fragment can be simplified into a conventional peptide fragment modified by a cross-linking agent fragment; 3) the enrichment of the cross-linked peptide fragment can be realized by the trehalose skeleton under the condition of not adding an enrichment handle; and 4) active groups of the cross-linking agent comprise but not limited to a plurality of reactive groups such as succinamide ester, diaziridine, phenylsulfonyl fluoride, hydrazide group, amino group, hydroxylamine group and the like, and chemical cross-linking of a plurality of amino acids except lysine is realized. The trehalose cross-linking agent disclosed by the invention is applied to the field of proteomics, and provides technical support for realizing large-scale analysis of a protein complex in a complex sample, spatial structure analysis of protein and a protein-protein interaction network.

The invention discloses a partial acylated non-natural sugar for metabolic labeling and application, the partial acylated non-natural sugar is a mannose type of a pyranose structure, and 1-hydroxyl and 6-hydroxyl are protected by hydrophobic groups. According to the method, the advantages of existing non-natural sugar are taken into consideration, it is guaranteed that the non-natural sugar can be efficiently utilized by cells, S side reaction of the non-natural sugar and cysteine in protein in the metabolism process is effectively avoided, and meanwhile efficient metabolism marking is achieved. In a cell test, the use concentration of the 1, 6-diacylated non-natural sugar is one order of magnitude lower than that of the non-natural sugar without the protecting group.

The invention discloses a production process of N-acetylglucosamine, and belongs to the technical field of N-acetylglucosamine production. Methanol is used as a solvent, glucosamine hydrochloride and acetic anhydride are used as raw materials, sodium hydroxide is added as a catalyst, other toxic chemical substances are not introduced, and meanwhile, the product yield is increased. In addition, in the product purification treatment process, a specific fixing agent is added, the specific fixing agent is a magnetic nanoparticle immobilized phenylboronic acid derivative, and the phenylboronic acid derivative can recognize and combine D-glucosamine with cis-dihydroxyl and cannot react with N-acetylglucosamine; the D-glucosamine in the N-acetylglucosamine can be effectively and selectively removed; and after the magnetic nanoparticles are immobilized, the selective adsorption reaction can be amplified, the separation and removal are facilitated, and the operation steps are simplified.

The invention discloses a vacuum concentration device for glucosamine preparation, and relates to the technical field of glucosamine preparation, the vacuum concentration device comprises a supporting bottom plate and a vacuum concentration tank body, the top of the vacuum concentration tank body is provided with a top cover, and the right side wall of the vacuum concentration tank body is provided with a vacuum pump which is communicated with the vacuum concentration tank body; the vacuum concentration tank is further provided with a separation assembly arranged in a communicated mode, a spraying cleaning component is arranged at the bottom of an inner cavity of the vacuum concentration tank, the inner cavity of the vacuum concentration tank is further provided with a mixing and scraping mechanism, the mixing and scraping mechanism comprises a servo motor and a cylinder, and the outer wall of the cylinder is connected with multiple sets of stirring blades, connecting rods and arc-shaped scraping plates. The mixing and scraping mechanism can drive the multiple sets of stirring blades to rotate, the heating effect on glucosamine liquid is good, the spraying and cleaning component and the mixing and scraping mechanism can rapidly scrape glucosamine raw material residues remaining on the inner wall of the vacuum concentration tank body, and the situation that the residual raw materials affect next-time glucosamine concentration is avoided; the concentration effect of the vacuum concentration device for preparing the glucosamine is improved.

The invention discloses a high-purity and high-yield separation and purification method of N-acetylglucosamine, and belongs to the technical field of biochemical engineering separation. The invention discloses a high-purity and high-yield separation and purification method of N-acetylglucosamine, which comprises the following steps: by taking fermentation liquor containing N-acetylglucosamine obtained by microbial fermentation as raw material liquor, performing flocculation pretreatment on the raw material liquor, and removing suspended particles, microorganisms, soluble macromolecular substances and the like through centrifugation or microfiltration, thereby obtaining the high-purity and high-yield separation and purification method of N-acetylglucosamine. And filtering to obtain clear filtrate. And performing concentration for three times, cooling and crystallizing the mixed solvent, and separating to obtain crude sugar. The raw sugar is re-dissolved and decolorized, and then is subjected to ion exchange desalination. And finally, performing concentration, cooling and crystallization, and performing forced air drying to obtain N-acetylglucosamine crystals with the purity of more than 99.5% and the total process yield of 75.0-87.2%.

The invention relates to the field of preparation of amino-oligosaccharides, and particularly discloses a chemical modification method of gingko oligosaccharides, prepared gingko amino-oligosaccharides and application of the gingko amino-oligosaccharides, the method comprises the following steps: S1, adding a hydrogen peroxide solution into the gingko oligosaccharides, and stirring to prepare a primary mixed solution, the glycosyl of the gingko oligosaccharides at least comprising glucose; s2, adding ammonium carbonate into the primary mixed solution, stirring, and stopping stirring until no bubble is generated in the solution; and S3, after standing reaction, drying to obtain the gingko amino-oligosaccharide. The invention also discloses the gingko amino-oligosaccharide prepared by adopting the modification method and application of the prepared gingko amino-oligosaccharide in plant growth. The ginkgo oligosaccharide is chemically modified, the molecular structure of the ginkgo oligosaccharide is connected with an amino functional group, the ginkgo oligosaccharide is converted into the ginkgo amino oligosaccharide, the function is more comprehensive and the effect is better due to the introduction of the functional group, and the efficiency of the ginkgo oligosaccharide in plant disease resistance and quality improvement is improved.

The application discloses an enzyme method for synthesizing unnatural sugar and application thereof, and belongs to the technical field of organic chemical synthesis. The unnatural sugar comprises an unnatural sugar obtained by introducing an acyl group into a hydroxyl group at the 6th position of a bare sugar through an acylation reaction; and the bare sugar is obtained by performing derivatization modification on an amide site of a natural sugar. The synthesis method uses a SubC enzyme as a catalyst to realize selective acylation of the hydroxyl group at the 6th position of the bare sugar, and synthesizes the unnatural sugar with an ideal yield. The unnatural sugar can be applied to glycomics metabolic labeling and mass spectrometry analysis as a marker, has excellent biological safety, specificity and labeling efficiency, can avoid non-specific labeling, and has no obvious cytotoxicity.

The utility model relates to the technical field of synthetic reaction devices, and discloses a synthetic reaction device for producing glucosamine sulfate.The synthetic reaction device comprises a bottom plate, a water bath box is fixedly installed at the top of the bottom plate, and a heating rod is fixedly installed in the water bath box; a synthesis stirring reaction mechanism and a reaction tank mounting mechanism are arranged inside and outside the water bath box. According to the synthesis reaction device for producing the glucosamine sulfate, by arranging the synthesis stirring reaction mechanism, in the using process, a stirring rod is arranged, and a first gear and a second gear are used for transmission, so that glucosamine sulfate production raw materials in the water bath synthesis reaction tank body can be fully stirred; and hot water in the water bath box can be stirred by utilizing the rotation of the rotating rod, so that the hot water is uniformly distributed, the condition of non-uniform water temperature distribution is avoided, and the water bath heating efficiency is improved.

There are provided, inter alia, methods for decreasing mucus elasticity or decreasing mucus viscosity in a subject in need thereof, the methods including administering to the subject an effective amount of a dithiolsaccharide mucolytic agent, and compounds and pharmaceutical compositions useful for the methods.

The invention relates to the technical field of novel drug application of compounds, in particular to a compound Man-Tz, a biological orthogonal reaction system and application of the compound Man-Tz. The dTRIM24 is accurately released at the atherosclerotic plaque through a biological orthogonal targeting technology, and the problem of non-specific distribution caused by a traditional administration mode is effectively solved by utilizing the specific reaction of an artificial target point Man-Tz and TCO-dTRIM24-TCO. According to the technology, the TRIM24 protein of 117kDa can be specifically degraded, and macrophages are promoted to be polarized to an anti-inflammatory M2 type by inhibiting Stat6 acetylation, so that plaque formation is inhibited. Experiments prove that the system has concentration and time-dependent effects, drug exposure of non-target tissues is remarkably reduced while efficient degradation activity is maintained, and a novel targeted therapy strategy with high specificity and low toxic and side effects is provided for atherosclerosis treatment.