4results about How to "Simple purification method" patented technology

This invention discloses a purification method for 2-bromo-1-(5-iodothiophene-2-yl)-ethyl ketone. Specifically, it includes the following steps: (1) slurrying crude 2-bromo-1-(5-iodothiophene-2-yl)-ethyl ketone in solvent A; (2) slurrying the product obtained in step (1) in solvent C; and (3) recrystallizing the product obtained in step (2) in solvent D. The purification method of this invention is simple and easy to implement, yields a product with high purity, and is suitable for industrial production.

An amide-containing aromatic diamine has the following structural formula: where Ar is a group with an aromatic ring structure. The preparation method includes the following steps: (1) mixing and reacting components including aromatic diamine, p-nitrobenzoic acid, alkyl phosphoric anhydride and polar aprotic organic solvent; (2) adding carboxylic acid and halogen-free quaternary ammonium salt to the product obtained in step (1) to obtain a mixed solution, which is placed in the cathode cell of an H-type electrolytic cell; the anode cell and the cathode cell are separated by a Nafion membrane; adding the same polar aprotic organic solvent and halogen-free organic electrolyte as the cathode cell to the anode cell of the H-type electrolytic cell, and adding a metal ion-free reducing agent; and conducting the reaction by electrolysis; (3) purifying the cathode reaction solution obtained in step (2) by post-treatment to obtain the amide-containing aromatic diamine. The yield, purity and whiteness of the amide-containing aromatic diamine are higher.

This invention relates to the field of materials science and technology, providing a porous gel scaffold for partitioned drug delivery, its preparation method, and its applications. The modification steps in this invention are simple, allowing for easy acquisition of the target product. The purification method is straightforward, and the modified monomers exhibit good biocompatibility and high safety. This invention modifies hyaluronic acid with norbornene and gelatin with thiol groups, effectively reducing its liquid viscosity and facilitating emulsification in liquid microfluidic chips. The synthesized microgels exhibit high uniformity in particle size, strong controllability, and stable drug loading concentration in a single gel. With the assistance of photocurable cyclodextrin, the aqueous solubility of non-water-soluble drug molecules can be significantly improved. The porous gel scaffold prepared by secondary assembly of the microgels used in this invention can better simulate the three-dimensional environment in vivo and can be used as a modular tissue engineering repair material for regenerative medicine drug / gene targeted delivery and stem cell loading.

This invention discloses a PTPσ_Ig1-3 fusion protein, its preparation method, and its applications, belonging to the field of genetic engineering. The recombinant fusion protein is obtained by adding an inducible peptide to the N-terminus of the PTPσ_Ig1-3 domain, and sequentially adding a TEV restriction site, GFP fluorescent protein, and a His tag to the C-terminus. The amino acid sequence of the PTPσ_Ig1-3 domain is shown in SEQ ID NO.1, the amino acid sequence of the inducible peptide is shown in SEQ ID NO.2, and the amino acid sequences of the TEV restriction site, GFP fluorescent protein, and His tag are shown in SEQ ID NO.3. Experimental verification showed that this recombinant fusion protein has significant binding activity with glycosaminoglycans such as heparin sodium and chondroitin sulfate, showing promising applications in the study of axonal injury and regeneration mechanisms and in drug delivery therapy.