8results about How to "Simple purification method" patented technology

This invention discloses a purification method for 2-bromo-1-(5-iodothiophene-2-yl)-ethyl ketone. Specifically, it includes the following steps: (1) slurrying crude 2-bromo-1-(5-iodothiophene-2-yl)-ethyl ketone in solvent A; (2) slurrying the product obtained in step (1) in solvent C; and (3) recrystallizing the product obtained in step (2) in solvent D. The purification method of this invention is simple and easy to implement, yields a product with high purity, and is suitable for industrial production.

The invention discloses a near-infrared fluorescent probe with an aggregation-induced emission enhancement effect. The near-infrared fluorescent probe is used for dual detection of hydrogen sulfide and hydrazine. The fluorescent probe is synthesized by carrying out Knoevenagel condensation reaction on 4-hydroxy-3-(1, 4, 5-triphenyl-1H-imidazolyl) benzaldehyde (I) and 2-(2-methyl-4H-chromene-4-subunit) malononitrile (II) under the catalytic action of piperidine, and the chemical structural formula of the fluorescent probe is as shown in the formula (III). The fluorescent probe shows high selectivity and high sensitivity to both hydrogen sulfide and hydrazine, the detection limits are as low as 50.1 nM and 89.2 nM respectively, and the fluorescent probe can be used for various scenes such as food spoilage monitoring and environmental water sample detection, can be used for biological imaging, and shows important application potential in the aspect of detection of hydrazine and hydrogen sulfide in organisms.

The application discloses a kind of methacrylic acid succinic acid double ester starch and its preparation method and application in corneal defect in situ filling agent, belong to the technical field of biomedical materials.The methacrylic acid succinic acid double ester starch is grafted carboxylation treatment to starch, then graft double bond is prepared.The corneal defect in situ filling agent of the application is composed of methacrylic acid succinic acid double ester starch (StacMA), methacrylated gelatin (GelMA), photo initiator and solvent.The natural biological macromolecule based corneal filling agent of starch and gelatin in the application has good biocompatibility, excellent light response adhesion characteristics and excellent mechanical properties, and the in-situ photocrosslinking of hydrogel is realized by ultraviolet irradiation, while excellent mechanical properties and strong adhesion capacity are generated, to realize the stable adhesion of in-situ forming artificial cornea on corneal implant bed, promote the regeneration of damaged corneal natural structure and the reconstruction of visual function.

An amide-containing aromatic diamine has the following structural formula: where Ar is a group with an aromatic ring structure. The preparation method includes the following steps: (1) mixing and reacting components including aromatic diamine, p-nitrobenzoic acid, alkyl phosphoric anhydride and polar aprotic organic solvent; (2) adding carboxylic acid and halogen-free quaternary ammonium salt to the product obtained in step (1) to obtain a mixed solution, which is placed in the cathode cell of an H-type electrolytic cell; the anode cell and the cathode cell are separated by a Nafion membrane; adding the same polar aprotic organic solvent and halogen-free organic electrolyte as the cathode cell to the anode cell of the H-type electrolytic cell, and adding a metal ion-free reducing agent; and conducting the reaction by electrolysis; (3) purifying the cathode reaction solution obtained in step (2) by post-treatment to obtain the amide-containing aromatic diamine. The yield, purity and whiteness of the amide-containing aromatic diamine are higher.

The invention discloses a synthesis method of (Z) type conjugated enol phosphate, which comprises the following steps: (1) synthesis of enol phosphate: reacting 4-chloroacetoacetate with phosphite ester, ending LC-MS (liquid chromatography-mass spectrometry) detection, and separating and purifying the product to obtain enol phosphate; and (2) synthesis of (Z) type conjugated enol phosphate: mixing enol phosphate, triethylamine and acetonitrile, stirring at room temperature for reaction, ending LC-MS detection, and separating and purifying the product to obtain the (Z) type conjugated enol phosphate. The method has the advantages of milder reaction conditions, higher efficiency, higher yield and better selectivity.

This invention discloses a PTPσ_Ig1-3 fusion protein, its preparation method, and its applications, belonging to the field of genetic engineering. The recombinant fusion protein is obtained by adding an inducible peptide to the N-terminus of the PTPσ_Ig1-3 domain, and sequentially adding a TEV restriction site, GFP fluorescent protein, and a His tag to the C-terminus. The amino acid sequence of the PTPσ_Ig1-3 domain is shown in SEQ ID NO.1, the amino acid sequence of the inducible peptide is shown in SEQ ID NO.2, and the amino acid sequences of the TEV restriction site, GFP fluorescent protein, and His tag are shown in SEQ ID NO.3. Experimental verification showed that this recombinant fusion protein has significant binding activity with glycosaminoglycans such as heparin sodium and chondroitin sulfate, showing promising applications in the study of axonal injury and regeneration mechanisms and in drug delivery therapy.

The invention belongs to the technical field of purification, and particularly relates to a method and a device for removing arsenic from yellow phosphorus. The method comprises the following steps: mixing crude yellow phosphorus, a first solvent and a rare earth oxide, dissolving, adding a second solvent into the obtained system, and carrying out anti-dissolution treatment to obtain a dissolved solution; sequentially carrying out cooling crystallization and separation on the dissolving solution to obtain crystals; melting the crystal and then removing rare earth oxide in the crystal to obtain yellow phosphorus after arsenic removal; the first solvent is a good solvent of yellow phosphorus; the second solvent is a poor solvent of yellow phosphorus. According to the method, a three-in-one technical route of solvent assistance, cooling crystallization and grain boundary capture is adopted, a mixed solvent system of a good solvent of yellow phosphorus and a poor solvent of yellow phosphorus is utilized, directional crystallization of yellow phosphorus is induced under a low-temperature condition in combination with the grain boundary modification effect of rare earth oxide, arsenic is forcibly left in a crystallization mother solution through a crystallization-impurity removal mechanism, and the yield of arsenic is increased. And thus, efficient removal of arsenic is realized.

This invention relates to the field of materials science and technology, providing a porous gel scaffold for partitioned drug delivery, its preparation method, and its applications. The modification steps in this invention are simple, allowing for easy acquisition of the target product. The purification method is straightforward, and the modified monomers exhibit good biocompatibility and high safety. This invention modifies hyaluronic acid with norbornene and gelatin with thiol groups, effectively reducing its liquid viscosity and facilitating emulsification in liquid microfluidic chips. The synthesized microgels exhibit high uniformity in particle size, strong controllability, and stable drug loading concentration in a single gel. With the assistance of photocurable cyclodextrin, the aqueous solubility of non-water-soluble drug molecules can be significantly improved. The porous gel scaffold prepared by secondary assembly of the microgels used in this invention can better simulate the three-dimensional environment in vivo and can be used as a modular tissue engineering repair material for regenerative medicine drug / gene targeted delivery and stem cell loading.