123 results about "Malononitrile" patented technology

The invention relates to the technical field of fluorescent probes, and provides a low-concentration ratio type high-concentration enhanced fluorescent probe as well as a preparation method and application thereof in order to solve the problems that a traditional fluorescent probe depends on a high-concentration use condition and is often faced with fast probe consumption, increased biotoxicity, non-specific target binding and the like in a biological sample. The preparation method of the fluorescent probe comprises the following steps: step 1, preparing phenothiazine dialdehyde; and 2, mixing phenothiazine dialdehyde, malononitrile, ethanol, acetonitrile, DMF and piperidine, and carrying out a reflux reaction to obtain the low-concentration ratio-type high-concentration enhanced fluorescent probe. The probe provided by the invention realizes quantitative detection of HClO through synchronous change of two emission channels, and the detection sensitivity and reliability are remarkably improved; when the concentration is high, an enhanced single-channel fluorescence amplification effect is shown, and strong signal identification and macroscopic visual imaging in specific application are facilitated, so that the dual requirements of trace monitoring and high-throughput screening are met.

The invention provides an imidazo [1, 2-alpha] pyridine stimuli-responsive molecule with a D-A twisted structure as well as a preparation method and application of the imidazo [1, 2-alpha] pyridine stimuli-responsive molecule, belongs to the technical field of intelligent materials, and is used for solving the technical problem that intelligent materials for developing stimuli-responsive molecules for data encryption and information anti-counterfeiting are insufficient. The preparation method of the compounds N1 and N3 comprises the following steps: 2-aminopyridine and 2, 4 '-dibromoacetophenone are subjected to an amphiphilic reaction to obtain a compound a; the compound a and POCl3 are subjected to a Vilsmeier-Haack reaction, and a compound b is obtained; carrying out Suzuki coupling reaction on the compound a or the compound b and triphenylamine boric acid to obtain a compound N1 or N2; the compound N2 and malononitrile are subjected to a Knovenagel condensation reaction, and a compound N3 is obtained. The compound prepared in the invention has good fluorescence emission capability in a solution and a solid state and a grinding solvent acid / alkali stimulated fluorescence discoloration behavior, and can realize good data encryption and information anti-counterfeiting.

The invention discloses a high-sensitivity ratio-type fluorescent probe for detecting hypochlorous acid as well as a preparation method and application of the high-sensitivity ratio-type fluorescent probe. The N '-(4'-(6, 6-dimethyl-4, 5, 6, 7-tetrahydro-1H-5, 7-bridge methylene indazole-3-yl)-[1, 1 '-biphenyl]-4-methylene) malononitrile prepared by using a natural renewable forest resource beta-pinene derivative nopinone as a raw material can selectively react with hypochlorous acid, and the fluorescent color of the N'-(4 '-(6, 6-dimethyl-4, 5, 6, 7-tetrahydro-1H-5, 7-bridge methylene indazole-3-yl)-[1, 1'-biphenyl]-4-methylene) malononitrile is changed from yellow to cyan, so that the effect of the N '-(4'-(6, 6-dimethyl-4, 5, 6, 7 the maximum fluorescence emission wavelength is changed from 460 nm to 375 nm, and the compound can be used as a specific ratio type fluorescent probe for detecting hypochlorous acid and has a good application prospect.

The invention relates to the technical field of organic photoelectric materials, and particularly discloses a non-condensed ring small molecule acceptor material based on a quinonoid structure as well as a preparation method and application of the non-condensed ring small molecule acceptor material. According to the invention, easily available 1, 4-diacetyl piperazine-2, 5-diketone is used as a center, an alkyl chain and Ar are used for modification, EG is used as an end group, the non-condensed ring A-D-A '-D-A type structure acceptor material based on a quinone structure is constructed, and the general formula is shown in the specification. Wherein the PCE of the acceptor material which is modified by bithiophene and takes 2-(3-ethyl-4-oxothiazolidine-2-subunit) malononitrile as a terminal group reaches 15%. The non-condensed ring small molecule acceptor material based on the quinonoid structure is simple in synthesis process, and an effective strategy is provided for solving the problem of the overall performance of an organic solar cell.

The invention discloses a lipid droplet specific fluorescent probe as well as a preparation method and application thereof, and relates to the technical field of biological medicines. The invention provides a structural formula of the lipid droplet specific fluorescent probe, and during preparation, 2-formaldehyde-6-(diethylamino) benzo [B] furan and a malononitrile derivative are used for preparing a fluorescent probe with an extended pi conjugated system under the action of an alkali environment; the fluorescent probe has the characteristic of intramolecular charge transfer (TICT), fluorescence in an aqueous solution is extremely weak, fluorescence in sunflower seed oil is remarkably enhanced, and the fluorescent probe has remarkable Stokes shift, excellent biocompatibility, excellent light stability and high signal-to-noise ratio. Meanwhile, the fluorescent probe shows extremely high sensitivity and signal-to-noise ratio in the aspect of cell lipid droplet imaging, and dynamic and accurate targeted imaging of lipid droplets can be easily realized. Therefore, the fluorescent probe disclosed by the invention is expected to become an alternative scheme for accurate detection of atherosclerosis.

The invention relates to the technical field of organic synthesis, in particular to a method for synthesizing cyclopentane compounds through [3 + 2] cycloaddition reaction of aryl cyclopropane and olefin under the synergistic catalysis of bromide ions / visible light. The method comprises the following steps: in a nitrogen atmosphere, sequentially adding aryl cyclopropane, a benzyl malononitrile derivative, pyridine tribromide and a photocatalyst into a reaction solvent to obtain a mixture, stirring the mixture at the temperature of 40 DEG C under the irradiation of a 12W blue light LED lamp until the reaction is complete, and performing concentration and column chromatography purification to obtain the cyclopentane compound. The method has the advantages of mild reaction conditions, simple operation, simple and easily available raw materials and low cost.

The utility model relates to the technical field of tail gas treatment, and discloses a malononitrile reaction tail gas treatment device which comprises a treatment box and a catalysis box, one side of the treatment box is provided with a first through hole, the inner wall of the first through hole is fixedly connected with a gas inlet pipe, and the inner side of the treatment box is slidably provided with an activated carbon filter element; a first sealing plate is mounted on one side of the activated carbon filter element through bolts, a first rectangular movable opening is formed in one side of the treatment box, and the first sealing plate is attached to the inner wall of the first rectangular movable opening. According to the device, tail gas can be conveniently adsorbed through activated carbon, gas adsorbed by the activated carbon filter element enters the catalysis box through the gas conveying pipe, and before the activated carbon filter element is used for adsorbing reaction tail gas, the service life of the activated carbon filter element can be prolonged by spraying and neutralizing the tail gas; the activated carbon filter element does not need to be frequently replaced, so that the cost is reduced, and the device is relatively practical and suitable for wide popularization and use.

The application discloses a catalyst for preparing malononitrile, a preparation method of the catalyst and a method for preparing malononitrile by using the catalyst. The catalyst is a solid catalyst, which is an acidic catalyst loaded with rare earth metal oxide. The method for preparing malononitrile is that cyanoacetic acid or cyanoacetate or cyanoacetamide and an ammonia source are subjected to ammoniation dehydration reaction in a continuous reactor to prepare malononitrile according to a certain molar ratio. Then, vacuum distillation (20 torr-25 torr) is carried out, and a fraction collected at 110 DEG C-120 DEG C is malononitrile product. The ammonia source can be ammonia gas, ammonia water, ammonium carbonate or urea. The method for preparing malononitrile can reduce production cost, improve production safety, reduce the discharge amount of three wastes in the preparation of malononitrile by using an existing process, and improve product quality, and is a sustainable and green synthesis route.

The invention discloses a benzothiazole-tetraphenyl ethylene derivative as well as a preparation method and anti-counterfeiting application thereof, 4-(1, 2, 2-triphenyl vinyl) acetophenone and o-aminothiophenol are reacted in dimethyl sulfoxide by taking I2 as a catalyst, and after the reaction is finished, separation and purification are performed to obtain a compound molecule 1-1. And 2, carrying out heating reaction on the obtained compound 1-1 and malononitrile under the action of alkali and a solvent, and separating and purifying to obtain a compound 1-2. The compound disclosed by the invention is low in preparation cost, simple and convenient to operate and simple in method, the compound shows fluorescence characteristics and can be applied to the fields of printing and dyeing, printing ink, cosmetics and the like, and meanwhile, the synthesis method can be used for synthesis in the fields of fine organic chemical engineering such as luminescent materials and trace detection technologies.

This invention belongs to the field of organic synthetic chemistry technology, specifically relating to a selenium-based indoloquinazoline derivative, its preparation method, and its application, comprising: in an organic solvent, using aromatic amine compounds, malononitrile, and diselenoether as raw materials, in the presence of a palladium catalyst and an organic base, at 90°C... o The reaction was carried out under C conditions with stirring to obtain a selenyl indoline quinazoline derivative. This method features mild reaction conditions, simple operation, high yield, and good functional group compatibility, conforming to the principles of green chemistry. Furthermore, the selenyl indoline quinazoline derivative provided by this invention exhibits good inhibitory activity against Plasmodium, providing a drug lead compound for the development of novel antimalarial drugs and holding significant importance for the prevention and / or treatment of malaria.

The method for high-efficiency catalysis of Knoevenagel condensation reaction by a porphyrin and quaternary ammonium salt synergistic catalytic system belongs to the technical field of catalyzing Knoevenagel condensation reaction. The synergistic catalytic system comprises a porphyrin ligand and a quaternary ammonium salt compound. The method for high-efficiency catalysis of Knoevenagel condensation reaction of aldehyde ketone and active methylene to generate methylene malononitrile compounds is to use the porphyrin ligand and the quaternary ammonium salt as a synergistic catalytic system to catalyze the condensation of aldehyde ketone and active methylene to generate methylene malononitrile compounds. The porphyrin ligand and quaternary ammonium salt synergistic catalytic system avoids the disadvantages of environmental pollution and difficult recycling of traditional basic homogeneous catalysts, so that the porphyrin ligand and quaternary ammonium salt synergistic catalytic system is easy to operate and recycle, has universal practicability, can be used by ordinary experimental operators, and provides an easy-to-operate, inexpensive and efficient route for the synthesis of methylene malononitrile compounds, which has a popular prospect.

The application discloses preparation of carbazolyl 3,5-dicyanopyridine derivatives, room temperature phosphor and anti-counterfeiting performance thereof. The preparation method comprises the following steps: adding ortho-, meta- or para-carbazolyl benzaldehyde and malononitrile into a reaction bottle, stirring and reacting under room temperature conditions with sodium hydroxide as a catalyst and anhydrous methanol as a solvent, and obtaining ortho-, meta- or para-carbazolyl 3,5-dicyanopyridine by separating and purifying the reaction crude product. Crystals of the three compounds are obtained by a solvent diffusion method with dichloromethane-petroleum ether as a solvent, and the crystal state room temperature phosphor material is obtained. The crystals are doped with urea / triphenylphosphine at a weight ratio of 1:100, the doped crystals are uniformly ground and mixed, and then heated to be molten, so that the host-guest doped phosphor material is obtained, and the novel material is used in the field of anti-counterfeiting patterns.

In order to solve the technical problems that the existing polymer separation membrane has poor anti-pollution performance and cannot be cleaned efficiently, the present application provides an ultrahydrophilic self-cleaning separation membrane and a preparation method thereof, specifically comprising: one, stirring amino malononitrile p-toluenesulfonic acid and manganese metal salt and adding them into an alkaline aqueous solution with a pH of 8.0-9.5 to obtain a mixed solution A; two, immersing a polymer membrane cleaned with alcohol and water step by step in the mixed solution A, and cleaning after coating; three, adding manganese metal salt into an alkaline aqueous solution with a pH of 8.0-9.5 to obtain a solution B; four, transferring the modified membrane obtained in the step two into the solution B, and cleaning after coating to obtain a modified membrane. The present application not only effectively gives the modified membrane ultrahydrophilicity, but also gives the modified membrane self-cleaning regeneration performance. The ultrahydrophilic self-cleaning modified membrane prepared by the present application can be widely applied in hydrophilic modification, oil-water separation, catalysis and other fields.

The present invention provides a D-D-π-A type organic photosensitizer and a synthesis method and application thereof, belonging to the field of photosensitizer and photodynamic therapy application of tumors. The present invention uses triphenylamine and carbazole as electron donors, malononitrile as an electron acceptor, and thiophene as an electron π bridge to synthesize an AIE type photosensitizer with mitochondrial targeting function through a series of simple organic reactions. The AIE type photosensitizer can be used in the synergistic photodynamic and ferroptosis treatment of tumors. The photosensitizer can realize tumor cell imaging and target mitochondria, and can selectively exert photodynamic and ferroptosis effects on tumor cells. It has good therapeutic effect and biosafety, and has strong application prospects in non-invasive tumor treatment.

The invention provides a preparation method of fluorodinitrile. The preparation method comprises the following steps: reacting trifluoromethanesulfonic anhydride with fluoroalkyl alcohol to obtain fluoroalkyl substituted trifluoromethanesulfonate; then, the fluoroalkyl substituted trifluoromethanesulfonate reacts with malononitrile, and fluorodinitrile is obtained; the reaction raw materials used in the method are easy to obtain and low in price, the target product conversion rate is high, byproducts are few, the purity of the prepared fluorodinitrile is as high as 99%, the yield is higher than 84%, the content of chloride and bromide ions is lower than 5 ppm, and the fluorodinitrile can meet the requirement of serving as an electrolyte additive.

The invention belongs to the field of Velciguat synthesis, and particularly relates to a preparation method of Velciguat and an intermediate product thereof. The preparation method of the Velciguat comprises the following steps: (1) reacting aminomalononitrile with methyl chloroformate to generate an intermediate product I; (2) carrying out ring closing on the intermediate product I and formamidine under an alkaline condition to obtain an intermediate product II; (3) carrying out bromination reaction on the intermediate product II and N-bromosuccinimide to obtain an intermediate product III; (4) reacting the intermediate product III with bis (pinacolato) diboron under the action of a catalyst to obtain an intermediate product IV; and (5) carrying out a reaction on the intermediate product IV and 5-fluoro-1-(2-fluorobenzyl)-3-iodo-1H-pyrazolo [3, 4-b] pyridine to prepare the viriciguat. The method is simple to operate, low in cost and safe in production. The invention also provides the intermediate product obtained by the preparation method.

The invention discloses a near-infrared fluorescent probe DCIC as well as a preparation method and biological imaging application thereof. According to the structure of the probe, (E)-2-(3-(3, 5-difluoro-4-hydroxystyryl)-5, 5-methylcyclohex-2-alkenyl) malononitrile is used as a near-infrared fluorophore, a dynamic response unit is constructed through acryloyl modification, and specific recognition and lasting signal output of Cys can be achieved. After the probe reacts with Cys, near-infrared fluorescence enhancement is generated at 650 nm, and the probe has the advantages of being short in response time, high in sensitivity and good in selectivity. Compared with the prior art, the rapid imaging of plant cells and living body Cys metabolism can be synchronously realized. The invention provides a high-sensitivity and high-temporal-spatial-resolution molecular tool for plant stress physiological research and disease-related Cys metabolism monitoring.

The invention belongs to the technical field of chemical analysis and detection, and provides a bimodal fluorescent probe and a preparation method and application thereof in hypochlorous acid detection, the chemical formula of the bimodal fluorescent probe is C68H44N2, and the preparation method comprises the following steps: weighing 2, 7-dibromo-9-fluorenone, 1-(4-phenylboronic acid pinacol ester)-1, 2, 4-triazole-1-one, 2, 4-triazole-1-one, 2, 4-triazole-1-one, 2, 4-triazole-1-one, 2, 4-triazole-1-one, 2, 4-triazole-1 The preparation method comprises the following steps: putting 1, 1, 2-triphenylethylene, a tetra (triphenylphosphine) palladium catalyst and potassium carbonate into a three-neck flask; sequentially injecting methylbenzene, absolute ethyl alcohol and water; carrying out heating reflux reaction, cooling to room temperature, carrying out extraction separation by using dichloromethane, collecting an organic phase, drying, carrying out rotary evaporation concentration to obtain a crude product, and purifying to obtain TPEO; dissolving TPEO and malononitrile in anhydrous dimethyl sulfoxide under the protection of argon, heating and stirring to react, cooling to room temperature, carrying out suction filtration, and washing the precipitate; the bimodal fluorescent probe can be applied to detection of hypochlorous acid, and has the advantages of specific selectivity, high sensitivity, wide detection concentration, high accuracy and the like.

The invention discloses an engineering continuous preparation method of 3-amino-4-amidoxime furazan, which is characterized in that engineering synthesis of 3-amino-4-amidoxime furazan is technically verified and optimized by adopting a continuous flow micro-channel reactor and a dynamic tubular reactor technology, and malononitrile is used as a starting raw material to react in the micro-channel reactor; the preparation method comprises the following steps: carrying out nitrosation and oximation to obtain a trioxime solution, conveying trioxime and a cyclization solution to a dynamic tubular reactor, heating to a certain temperature, reacting and curing, overflowing into a collection tank through the tubular reactor, adjusting the pH value of a reaction solution, cooling, filtering, washing with water, and drying to obtain the 3-amino-4-amidoxime furazan. The AAOF engineering manufacturing process scheme is provided, AAOF kilogram-level engineering preparation under automatic control is achieved, the problems of material precipitation, heat exchange and the like in an intermittent production mode are solved, and the synthesis efficiency and the synthesis safety are greatly improved.

This application discloses a method for synthesizing chiral cyano-containing full-carbon quaternary cyclic compounds, characterized by the following steps: mixing a solvent, an oxidant, an alkynyl malononitrile compound, a carboxylic acid compound, a palladium salt, and a chiral dinitrogen ligand; reacting the mixture; drying; and separating to obtain the chiral cyano-containing full-carbon quaternary cyclic compound. This method can achieve a palladium-catalyzed tandem reaction of alkynyl malononitrile with a carboxylic acid involving asymmetric acyl oxidative cyclization / acyl migration, yielding cyano-containing full-carbon quaternary cyclic compounds with optical purity in good yields (enantiomer ratios up to 96.5:3.5). This invention features a broad substrate range, readily available raw materials, simple and practical operation, and mild reaction conditions.

The utility model discloses distillation equipment for malononitrile production. The distillation equipment comprises a rack, the right side of the upper surface of the rack is provided with an evaporation chamber, the upper end of the evaporation chamber is provided with a steam pipe, the left end of the steam pipe is connected with a condensation chamber, a spiral pipe is arranged in the condensation chamber, the two ends of the spiral pipe extend out of the condensation chamber, the right end of the spiral pipe is connected with a first liquid inlet pipe, and the right end of the first liquid inlet pipe extends to the lower end in the evaporation chamber. A shell is fixedly arranged outside the condensation chamber in a sleeving mode, the left end of the spiral pipe is communicated with the interior of the shell, a liquid supply pipe is arranged at a liquid inlet hole in the right end of the outer arc face of the shell, a spiral piece is arranged between the inner arc wall of the shell and the outer arc face of the condensation chamber, a controller is arranged on the front side face of the rack, and a heating disc is arranged on the lower surface of the evaporation chamber. According to the distillation equipment for malononitrile production, a solution to be evaporated can be preheated while steam is cooled, the heat exchange effect is good, energy waste is reduced, and the production cost is reduced.

The invention relates to the field of preparation of drug intermediates, and particularly discloses a synthetic method of a drug intermediate pyrazolo [1, 2-b] phthalazine-5, 10-diketone derivative and a catalytic system for synthesis. According to the synthetic method, aromatic aldehyde, phthalylhydrazine and malononitrile are used as reaction raw materials; the pyrazolo [1, 2-b] phthalazine-5, 10-diketone derivative is synthesized under the catalytic action of a catalytic system consisting of an alkaline eutectic solvent and absolute ethyl alcohol. The content of each component and reaction process parameters are optimally designed, so that the utilization rate of the reaction raw materials and the yield of the pyrazolo [1, 2-b] phthalazine-5, 10-diketone derivative can be remarkably increased, and the economical efficiency is relatively good; the catalytic system is high in catalytic activity, the usage amount in the catalytic process and the loss amount of the catalyst during recycling are small, the number of times of recycling is large, the catalytic system does not need to be treated before recycling, and operation is easy.

The invention discloses a quinoline-malononitrile derivative, a preparation method thereof and application of the quinoline-malononitrile derivative as a near-infrared AIE fluorescent probe of a targeted lysosome, and belongs to the technical field of fluorescent probes. According to the quinoline-malononitrile derivative, quinoline-malononitrile serves as a core fluorophore, a thiophene group and a morpholine group are introduced for conjugate connection, an alkyl sulfonic acid group is further modified, and the quinoline-malononitrile derivative is a near-infrared AIE fluorescent probe, has good biocompatibility and stability, has targeting performance on lysosome, shows bright fluorescence in MCF-7, and can be used as a fluorescent probe. The quinoline-malononitrile derivative shows weak fluorescence in HUVEC, can generate fluorescence response according to the fluctuation of lysosome viscosity and can realize accurate imaging of a tumor microenvironment, and the structure of the quinoline-malononitrile derivative is as follows: # imgabs0 #.

Organic photovoltaic cells (OPVs) and their compositions are described herein. In one or more embodiments, the acceptor with an active layer of an OPV includes is a non-fullerene acceptor. Such non-fullerene acceptors may provide improved OPV performance characteristics such as improved power conversion efficiency, open circuit voltage, fill factor, short circuit current, and / or external quantum efficiency. One example of a non-fullerene acceptor is (4,4,10,10-tetrakis(4-hexylphenyl)-5,11-(2-ethylhexyloxy)-4,10-dihydro-dithienyl[1,2-b:4,5b′]benzodi-thiophene-2,8-diyl) bis(2-(3-oxo-2,3-dihydroinden-5,6-dichloro-1-ylidene) malononitrile.

The invention discloses a fluorescent probe for detecting hydrogen sulfide as well as a preparation method and a use method of the fluorescent probe. According to the preparation method, (E)-2-(3-(3-formyl-4-((5-nitropyridine-2-yl) oxy) styryl)-5, 5-dimethylcyclohex-2, 4-diene-1-subunit) malononitrile is used for constructing a classical photoinduced electron transfer (PET) system, and the photoinduced electron transfer system is used for preparing the photoinduced electron transfer material. The probe blocks the transition of electrons from an excited state to a ground state due to the existence of 2-chloro-5-nitropyridine, the probe has a classical PET effect and does not emit fluorescence, in the presence of hydrogen sulfide, hydrogen sulfide firstly attacks an aldehyde group in a nucleophilic manner to form a sulfydryl structure, then sulfydryl molecules attacks a carbon atom at a site 2 on a pyridine ring in a nucleophilic manner, and the sulfydryl structure is formed. When hydrogen sulfide is detected, ether bonds are initiated to break to release fluorophores, a strong ICT effect is formed, red fluorescence is emitted, and high-sensitivity and specific detection of hydrogen sulfide is realized.

The preparation method comprises the following steps: S1, adding cellulose acetate, MXene (at) Ag2S heterojunction, amidoxime modified malononitrile and an organic macromolecular additive into an organic solvent, stirring to obtain a membrane casting solution, and standing the prepared membrane casting solution to remove bubbles, so as to obtain a membrane casting solution; casting a layer of membrane casting solution on plate glass, then immediately immersing the plate glass into water, and taking out the plate glass after the plate glass is completely cured to obtain a photocatalytic membrane; wherein the organic macromolecular additive is one of polyvinylpyrrolidone, polyethylene glycol and polyvinyl alcohol; and S2, immersing the photocatalytic membrane into an aqueous solution of glycidyl dimethyl dodecyl ammonium chloride, and carrying out a surface grafting reaction to obtain the long-chain alkyl quaternary ammonium salt grafted amidoxime modified MXene coated Ag2S photocatalytic membrane. The seawater uranium extraction performance of the MXene photocatalytic membrane material is integrally improved.

The present invention discloses a method for preparing an α-arylimino aryl acetonitrile derivative, comprising the following steps: (1) using ethanol as a solvent, mixing an aromatic aldehyde and an aromatic amine to react to generate a Schiff base compound; (2) using cesium carbonate as a base, anhydrous N,N-dimethylformamide as a solvent, and malononitrile as a cyanogen source to mix with the Schiff base compound generated in step (1), heating to 90-100° C., and reacting for 7-8 hours to obtain an α-arylimino aryl acetonitrile derivative. The method synthesizes a target compound by reacting aromatic aldehyde, aromatic amine, and malononitrile under the action of cesium carbonate. The method has the advantages of simple reaction steps, using a green cyanogen source malononitrile, avoiding the use of highly toxic reagents such as potassium cyanide and trimethylsilyl cyanide, convenient post-processing, high product regioselectivity, and being able to effectively construct the basic skeleton of the α-arylimino aryl acetonitrile.

The invention discloses an RNA (Ribonucleic Acid) fluorescent probe as well as a preparation method and application thereof in cell nucleolus staining, and belongs to the technical field of biomedical detection. The probe is a water-soluble pyridine salt compound, the chemical name of the probe is 1-(2-(2-(2, 2-dicyanovinyl))-5-(dimethylamino phenoxy) ethyl) pyridine-1-ammonium bromide, and the preparation method of the probe is characterized in that 2-(2-bromoethoxy)-4-(dimethylamino) benzaldehyde and malononitrile are used as raw materials, and the probe is synthesized through two-step reaction. The probe has the characteristics of large Stokes shift, low cytotoxicity and fluorescence enhancement after being combined with RNA (Ribonucleic Acid). Experiments prove that the probe can specifically carry out efficient fluorescence labeling on nucleolus and cytoplasm RNA in fixed cells, and a high-contrast imaging effect is obtained. The probe disclosed by the invention is simple and convenient to synthesize and excellent in performance, and has important application value in the fields of cell nucleolus research, tumor diagnosis and the like.

The present application relates to the field of coating, disclose a kind of preparation method of functional coating based on aminomalonic nitrile self-polymerization, comprising: S1: modifying hyaluronic acid to introduce aldehyde group, obtain aldehyde group hyaluronic acid;S2: amino malonic nitrile p-toluenesulfonic acid salt is dissolved together with aldehyde group hyaluronic acid in tris-hydroxymethyl aminomethane hydrochloride buffer solution and mixed, obtain functional coating solution;S3: substrate is immersed in functional coating solution, after oscillation incubation, remove, clean, dry, obtain the substrate that surface is uniformly coated with functional coating.The present application first carries out aldehyde group modification to hyaluronic acid to give its copolymerization ability with amino malonic nitrile p-toluenesulfonic acid salt, then it is mixed with amino malonic nitrile p-toluenesulfonic acid salt under alkaline condition, and is deposited on substrate surface by oscillation reaction, and the coating with anti-inflammatory function is prepared.

The invention discloses a quinoline-malononitrile mother nucleus-based photosensitizer as well as a preparation method and application thereof. The structure of the photosensitizer is characterized in that a quinoline nitrile fragment is taken as an electron acceptor, triphenylamine and substituted triphenylamine fragments are taken as electron donors, and a carbon-carbon double bond or thiophene heterocyclic ring is taken as a pi bridge to connect the electron acceptor and the electron donor. Meanwhile, the invention further discloses a preparation method of the photosensitizer and potential biological application of killing tumor cells through the photodynamic effect.