Fluorescent compound and application thereof in hypochlorous acid detection

Abstract

The invention belongs to the technical field of analysis and detection and discloses a fluorescent compound and the application thereof in hypochlorous acid detection. The fluorescent compound is 2-{3-cyan-4-[2-(4'-{2-[4-(2-hydroxy-ethyoxyl)-phenyl]-1,2-biphenyl-vinyl}-biphenyl-4-yl)-vinyl]-5,5-dimethyl-5H-furyl-2-ylidene}-malononitrile, and has a structural formula as shown in formula (1). By the adoption of the fluorescent compound, enhancement mode fluorescence detection of hypochlorous acid can be achieved, interference of external conditions can be avoided during analysis and detection, and detection precision and accuracy are high; the fluorescent compound can be prepared into test paper which is convenient to carry and store and capable of achieving qualitative testing conveniently, and fluorescence quenching occurring when traditional fluorochrome is used as a solid material is avoided. The fluorescent compound can be used for qualitative and quantitative analysis of hypochlorous acid in chemical and environmental samples as well as other samples.

Description

technical field [0001] The invention belongs to the technical field of analysis and detection, and in particular relates to a fluorescent compound and its application in the detection of hypochlorous acid. Background technique [0002] Hypochlorous acid and its conjugate base HClO / ClO - As a strong oxidant, it is used in various organic synthesis, and is also used as a disinfectant and bleaching agent to achieve antifouling of the cooling water system of power stations and disinfection of tap water and drinking water. However, when hypochlorous acid is used for disinfection of tap water and drinking water, some organic chlorine by-products (OBPs) will be produced in the water, which will cause certain harm to the human body. At the same time, if the concentration of OBPs is too high, it will also cause oxidative stress in aquatic organisms, and eventually cause genotoxicity in aquatic organisms. Although ClO - It has a certain bactericidal effect, and aquatic organisms al...

AI Technical Summary

Problems solved by technology

Chinese patent CN201210528045.7 prepared diphenylselenyl derivatives based on naphthalimide. This compound reacts with hypochlorous acid to form selenium oxide. Under the irradiation of excitation light, fluorescence enhancement can occur in the wavelength range of 500nm–575nm. In this way, the fluorescence-enhanced detection of hypochlorous acid is realized; however, the detection system is easily interfered by other oxides, and the fluorescent probe has the property of aggregation-induced quenching, which makes it difficult to achieve fluorescent test paper detection

Fluorescence ratiometric detection method (for example published paper ACS Appl.Mater.Interfaces, 2016, 8, 1511–1519), synthesized a fluorescent probe based on pyrene indole derivatives, under 430nm laser irradiation, the The probe molecule emits red fluorescence at 613nm. After the addition reaction of the double bond and hypochlorous acid, the indole derivative of pyrene becomes the aldehyde derivative of pyrene, so that under the same excitation light, the 470nm The blue fluorescence intensity increased significantly, while the red fluorescence at 613nm was greatly weakened, realizing the fluorescence ratiometric detection of hypochlorous acid; however, the fluorescent probe has the property of aggregation-induced quenching, which makes it difficult to realize the fluorescent test paper detection

Images