45 results about "Biological sciences" patented technology

The AI-enhanced cellular modeling and simulation platform is a computational system designed to enhance biomedical research and development and personalized medicine and wellness. This platform integrates simulation modeling, machine learning and artificial intelligence, multi-omics data, and sophisticated data fusion and decision-support techniques to create comprehensive models of cellular systems and processes across multiple scales. It enables researchers and clinicians to simulate complex biological interactions, predict disease progression, and design or optimize treatment strategies or medical devices with improved accuracy and efficacy. The system's architecture allows for integration of various components, including real-time data processing, federated learning, and quantum computing enhancements. From personalized drug discovery and cancer therapies to synthetic biology and epidemiological analysis, this platform offers powerful tools for understanding and manipulating cellular systems and bioengineered systems. By bridging the gap between molecular-level interactions between cells and materials and organism-wide effects, it enables significant advancements in healthcare and biological sciences.

The invention discloses a biological characteristic analysis system based on multi-modal data and a residual image attention network. The biological characteristic analysis system comprises a multi-modal data processing module, an individual similarity network construction module, a biological characteristic extraction module and a biological characteristic analysis module. Clinical image data and multi-modal data of gene expression are collected, on one hand, a comprehensive biological individual similarity network is fused and constructed, on the other hand, feature extraction is carried out based on a multi-modal auto-encoder fusion method, and finally, a constructed residual image attention network model is input for feature processing. Therefore, accurate classification and analysis of various biological characteristics and samples can be realized. The biological potential feature analysis accuracy and reliability are effectively improved, powerful reference is provided for biological scientific research and health management, and early discovery of potential changes of biological features and implementation of personalized health management are facilitated.

The application discloses a high-expression-based CRM197 protein specific neutralization detection method and belongs to the technical field of biological detection. The method comprises the following steps: S1, strain resuspension: Corynebacterium diphtheriae is resuspended to obtain a bacterial suspension; S2, bacterial liquid culture: the bacterial suspension is inoculated into an iron trichloride YC liquid culture medium to be cultured to obtain a bacterial liquid; and S3, specific neutralization: the bacterial liquid is inoculated on Elek's medium to be subjected to virulence determination. The application establishes a novel CRM197 protein specific neutralization detection method, greatly improves the technical requirements of detection effect and time limit, and provides a novel detection method for biological science.

The application discloses a seminal plasma metabolic biomarker combination for male infertility spermatogenesis obstacle typing diagnosis, and relates to the field of biological science and technology.The application records the metabolic fingerprints of 475 seminal plasma samples by means of nanoparticle-enhanced laser desorption / ionization mass spectrometry, and constructs a seminal plasma metabolic fingerprint database related to spermatogenesis obstacle (SPGF). Then, through machine learning and statistical analysis, six metabolic biomarkers for SPGF typing diagnosis are screened and identified in the seminal plasma, and the six metabolic biomarkers can realize accurate typing of SPGF (AUC=0.814). The application has the advantages of simple sample pretreatment, fast analysis speed and low cost by means of recording the metabolic fingerprints of seminal plasma by means of nanoparticle-enhanced laser desorption / ionization mass spectrometry. Through the synthesized iron oxide nanoparticles as a matrix, small molecule metabolites in the seminal plasma can be efficiently captured and ionized, and complex pretreatment steps such as deproteinization and chromatographic separation are not needed, so that super-fast analysis, super-high sensitivity, extremely low sample consumption and excellent repeatability detection are realized.

A reaction tube for multiple nucleic acid amplification is relevant to the field of application technology in life science research and medical examination, and the like. This reaction tube for multiple nucleic acid amplification comprises a base (1) and several reaction tube cavities (3); the base (1) is provided with a reference plane (2), the openings of the several reaction tube cavities (3) are all located on the reference plane (2), and their interiors extend perpendicularly to the reference plane (2) towards the interior of the base (1).In the reaction tube for multiple nucleic acid amplification, several reaction tube cavities (3) are provided on the reference plane (2) of the base (1). Multiple PCR amplification can be carried out by adding a nucleic acid sample and different PCR systems, or alternatively, a PCR freeze-drying system is included in the tube cavity (3). Depending on requirements, a nucleic acid sample for amplification is distributed in different reaction tube cavities (3), and several different types of nucleic acids can be amplified simultaneously and stored under the same reaction environment or made available for other reaction tests, thereby significantly increasing the efficiency of nucleic acid amplification and ensuring uniformity of conditions for nucleic acid amplification.

The invention belongs to the technical field of fluorescent probes, and particularly relates to a fluorescent probe for detecting pyrophosphoric acid or pyrophosphate based on ratiometric fluorescence, a preparation method and application. According to the fluorescent probe, cyclotriphosphazene is taken as a core platform, a naphthalimide-triphenylamine luminophore and a terpyridine recognition unit are introduced, a multi-arm fluorescent probe is integrally formed, coordination terpyridine interacts with metal ions Zn < 2 + >, fluorescence signals are induced to change, and therefore sensitive detection on PPi is achieved. The fluorescent probe has the advantages of quick response, high selectivity, interference resistance and low cost, and has important application value in the fields of environmental science, biological science and the like.

The utility model relates to the technical field of biological science experimental equipment, in particular to sample preservation equipment for IV-type collagen detection, which comprises a base, a box body is rotatably connected to the inner wall of the base, a box cover is in threaded connection with the surface of the box body, an inner box is rotatably connected to the inner wall of the box body, and the inner box is in threaded connection with the box cover. The lower end of the inner box is fixedly connected with the inner wall of the base, two symmetrically-arranged through grooves are formed in the surface of the inner box, two symmetrically-arranged sliding rods are fixedly connected to the surface of the inner box, and two symmetrically-arranged sliding grooves are formed in the inner wall of the box body. According to the sample tube fixing device, the sample tubes are effectively fixed, the sample tubes are tightly clamped by the fan-shaped blocks in the closed state of the device, the sample tubes are prevented from falling off due to shaking or external force, the stability of the sample tubes in the storage process is guaranteed through the fixing mode, and damage caused by mutual collision of the sample tubes is avoided.

This invention belongs to the interdisciplinary field of bioscience and information technology, and relates to a method for assessing the intensity of gene transcriptional regulation based on multi-omics data. Addressing the challenges of systematically integrating multi-omics data and quantitatively characterizing the correspondence between regulatory elements and genes, as well as the intensity of regulation, in existing transcriptional regulation analyses, this invention integrates epigenomics, transcriptomics, and three-dimensional genomics data to construct a site-gene regulatory intensity model. This method extracts candidate cis-regulatory sites and calculates their regulatory activities. It then determines the site-gene regulatory weights by combining distance weighting and three-dimensional genomic contact information, thereby obtaining the regulatory intensity at the site-gene pair and gene levels. An iterative algorithm distinguishes between positive and negative regulatory effects, achieving correction and summarization of regulatory intensity. This invention can quantitatively assess the regulatory intensity of cis-regulatory elements on target genes and the overall regulatory effect on genes, and is suitable for studying gene transcriptional regulation mechanisms and analyzing gene expression changes under different treatment conditions.

The invention relates to the field of biological science and probiotic engineering, in particular to a non-inducible promoter applied to probiotics and application of the non-inducible promoter, the promoter can achieve stable and efficient expression of a target gene in probiotics preferably EcN1917 without any external induction operation, and therefore the functional characteristics of the EcN1917 serving as the probiotics are remarkably improved.

1. The name of the design product: cell detector (handheld). 2. The use of the design product: the design product is used for biological science detection and analysis. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective view.

The utility model relates to the technical field of biological science, in particular to a spiral microbial reactor, which comprises moving seats, locking devices, fixing devices, inserting devices and bent pipes, straight pipes are fixed by the fixing devices, then the straight pipes move on a support plate through a plurality of moving seats and are locked by the locking devices, and the inserting devices are inserted into the straight pipes. The distance between every two adjacent straight pipes is equal to the distance between the two ends of the corresponding bent pipe, finally, the bent pipes and the straight pipes are connected through the inserting devices, the spiral pipe reactor can be installed, and compared with the prior art, installation of a plurality of pipelines can be completed.

The invention belongs to the technical field of fluorescent probes, and particularly relates to application of a triphenylamine-terpyridyl fluorescent probe to detection of nucleotide and / or chain-shaped polyphosphoric acid. The fluorescent ligand TPA-3TPY provided by the invention is chelated with # imgabs0 # ions in situ to successfully prepare the metal complex, so that the fluorescence emission wavelength moves towards the long wave direction. When the nucleotide and the derivative thereof are detected, the # imgabs 1 # ion is separated from the fluorescent ligand, and the fluorescence emission wavelength is recovered, so that the effective detection of the nucleotide and the derivative thereof is realized. The method has the advantages of quick response, high selectivity, interference resistance and low cost, and has important application value in the fields of food science, environmental science, biological science and the like.

The invention belongs to the technical field of fluorescent probes, and particularly relates to a benzothiazole fluorescent molecule as well as a preparation method and application thereof. The benzothiazole fluorescent molecule as shown in the structural formula (1) provided by the invention is synthesized by taking amino thiol and salicylaldehyde as raw materials. The benzothiazole fluorescent molecule is selectively and specifically combined with a boron-containing compound, the fluorescence intensity is remarkably enhanced, open-type detection of the boron-containing compound is realized, and the benzothiazole fluorescent molecule has the advantages of quick response, high selectivity, interference resistance and low cost, and has important application value in the fields of environmental science, biological science, material engineering and the like.

The invention discloses a serum metabolism marker combination for ectopic pregnancy diagnosis and rupture risk prediction and application of the serum metabolism marker combination, and relates to the technical field of biological science. The metabolic fingerprint diagnosis model (AUCgt, 0.91) and the metabolic marker panel (AUCgt, 0.92) have the advantages that the performance of the metabolic fingerprint diagnosis model (AUCgt, 0.91) and the performance of the metabolic marker panel (AUCgt, 0.92) are obviously superior to those of an existing'ultrasonic + beta-hCG 'diagnosis mode, the problems of diagnosis delay, misdiagnosis and missed diagnosis clinically at present are effectively solved, and the clinical rigid requirements of early and accurate diagnosis are met; the fracture risk prediction model AUC 0.885 constructed by the method disclosed by the invention fills the current clinical blank. Key decision support can be provided for doctors, early intervention on high-risk patients is achieved, excessive medical treatment on low-risk patients is avoided, and the mortality risk of pregnant and lying-in women is reduced. According to the invention, based on the nanoparticle-assisted laser desorption / ionization mass spectrometry technology, ultra-fast detection of only about 3 seconds for single sample detection is realized, and the method is expected to become a fast detection tool in emergency treatment and outpatient service scenes.

The invention discloses a plasma metabolism biomarker combination for predicting and intervening propofol-induced respiratory depression, a detection method and application, and relates to the technical field of biological science. According to the method, non-target metabonomics detection is conducted on preoperative plasma of 298 subjects through UPLC-MS, 14-metabolite panels are obtained through machine learning supervised feature screening, a diagnosis model is established through verification of discovery and verification queues, and the prediction AUC of severe respiratory depression reaches 0.86-0.91 (discovery queue) and 0.86-0.91 (verification queue). Mouse in-vivo function verification proves that the key metabolite alpha-linolenic acid obviously improves the respiratory rate, the tidal volume and the minute ventilation volume under two administration paradigms of pretreatment and treatment of a mouse propofol-RD model, shortens the recovery time of righting reflex, has a good safety foundation, and has a good application prospect. Prompt can be converted into a foresight supplement or rapid treatment medication thought in a perioperative period, and a'prediction and intervention 'integrated clinical value is formed.

The invention discloses a DNA methylation recognition method based on deep learning and multi-scale feature fusion, and belongs to the crossing field of artificial intelligence and bioscience, the method comprises the following steps: obtaining original data of a DNA sequence to be detected, and carrying out preprocessing and feature extraction on the original data to obtain a time-frequency feature matrix; inputting the time-frequency feature matrix into a pre-trained multi-scale feature fusion network, wherein the multi-scale feature fusion network performs classification through RepVGG modules with different expansion rates arranged in parallel, 1 * 1 convolution and a full-connection network to obtain a classification result; and determining the methylation state of the DNA sequence based on the classification result.

The utility model discloses a glass slide arrangement frame, which belongs to the technical field of biological science and comprises a box body, a fixing frame is fixedly connected onto the inner wall of the box body, a double-shaft motor is fixedly connected onto the fixing frame, two output shafts of the double-shaft motor are both fixedly connected with a right-hand threaded column and a left-hand threaded column, and the right-hand threaded column and the left-hand threaded column are fixedly connected onto the left-hand threaded column. And one end of the right-hand threaded column and one end of the left-hand threaded column are both fixedly connected with rotating shafts, one ends of the two rotating shafts are both sleeved with first bearings, and the two first bearings are both fixedly connected to the inner wall of the box body. The device has the beneficial effects that by arranging a rotating plate, a first threaded column and a threaded hole, the rotating plate drives the first threaded column to rotate in the threaded hole by rotating the rotating plate, so that a limiting rod moves in a limiting hole, and the first threaded column drives a U-shaped movable seat to get close to a corresponding U-shaped placement groove; the glass slide can be conveniently limited and fixed through the U-shaped movable seat and the U-shaped placing groove, so that the glass slide is kept stable.

The present invention discloses an intelligent monitoring device and method for airborne fungal spores and meteorological factors, which relate to the fields of intelligent agricultural machinery and equipment and biological sciences, and include: a microscopic image acquisition mechanism, a spore capturing air duct mechanism, a stage and a slide magazine mechanism. The present invention can realize aerial spore capturing and microscopic image acquisition in a field environment. The monitoring device and method of the present invention solve the problem that the spore concentration in the farmland cannot be automatically, real-time and on a large scale monitored, which makes it difficult to grasp the real-time and dynamic changes of fungal spores in the farmland on a large scale. The spore microscopic images magnified 100 to 1000 times can be collected, the work flow is smooth and the operation is simple, and the application prospect is broader.

The invention belongs to the technical field of fluorescent probes, and particularly relates to application of a cyclotriphosphazene-terpyridyl fluorescent probe to detection of flavonoid substances. The invention provides a fluorescent probe for detecting flavonoid substances. The fluorescent probe belongs to cyclotriphosphazene-terpyridyl compounds. The fluorescent probe has a fluorescence red shift phenomenon after being combined with a flavonoid substance, realizes ratio type fluorescence detection, has the advantages of quick response, high selectivity, interference resistance and low cost, provides a new direction for detection of the flavonoid substance, and has important application value in the fields of environmental science, biological science and the like.

The present application relates to the field of biological science and probiotic engineering, and particularly relates to a non-inducible promoter applied to probiotics and application thereof, the promoter can realize stable and efficient expression of a target gene in probiotics, preferably EcN1917, without any external induction operation, thereby significantly improving the functional characteristics of EcN1917 as probiotics.

The utility model relates to a hymenoptera insect smell selectivity experiment device, which belongs to the field of biological science experiment instruments and comprises a movable search box, a transparent top cover is movably attached to the top end of the movable search box, and an experiment mechanism is arranged in the movable search box. According to the hymenoptera insect smell selectivity experiment device, a tested smell source is placed in the two selection chambers respectively, the transparent top cover is quickly screwed after the smell source is placed in, the straight opening end of the inlet pipe and the connecting opening are fixed, tested insects are released, after the tested insects enter the movable search box, selection can be carried out according to the smell emitted by the selection chambers, and then the tested insects can be selected according to the smell emitted by the selection chambers. After being selected according to preferences, the test insects enter through the small ventilation holes, and finally, data such as the number of the test insects entering the small selection chamber or the selection time of a single test insect in the set time are counted, so that the purpose of conveniently carrying out multiple groups of experiments is achieved, and the problems that multiple groups of experiments are inconvenient to carry out at the same time and the actual operation cost is high are solved.

The application discloses a kind of biological scientific research data collaborative analysis system based on cloud platform, it is related to data analysis technical field, including management center, the management center is connected with basic acquisition module, platform processing module, automation analysis module and service support module;To biological scientific research data data transmission change, obtain biological scientific research signal, to biological scientific research signal is dimension conversion, obtains biological wave variation array;Through updating prediction array, biological wave variation array is replaced with number and number chart conversion, obtains the graph of biological science, sets up mobile capture axis and obtains identity characteristic section to the graph of biological science;According to position node interval, identity characteristic section is distinguished by type, obtains biological identity type library, filters combination to biological identity type library, obtains biological type sequence;Efficient data processing is carried out using cloud platform, optimization collaboration experience is ensured Data security.

The present application relates to the field of biological science, and in particular to a method for assembling high-quality goose genome sequence using multiple sequencing technology means. The method uses Pacific Biosciences (PacBio) HiFi reading, Ont nanopore super-long reading, Illumina short reading and chromatin conformation capture (Hi-C) multiple sequencing technology means, and successfully assembles high-quality goose genome sequence. The assembled goose T2T chromosome level genome lays an important research foundation for future genetic improvement and genetic mechanism analysis of geese.

This application belongs to the field of biological science research and big data mining technology, and discloses a system and method for integrating and analyzing multi-omics data of environmental endocrine disruptors. Through data collection, data storage, integrated analysis and visualization, a dedicated metadata framework for environmental endocrine disruptors is constructed, and a full-dimensional collection system covering chemical data and multi-omics data is established to realize multimodal data integration analysis and visualization functions, thereby promoting the prediction and query analysis of EDCs toxicity mechanisms. This application supports automatic conversion and structured storage of multiple data formats, thereby significantly improving data traceability efficiency and shortening the data preprocessing cycle.

The application discloses a kind of methods of super-resolution large depth three-dimensional scanning light sheet microscope, belong to fluorescence imaging technical field, the excitation light is handled by column lens in the present application, the longer excitation objective working distance is obtained using the secondary convergence of the initial light sheet generated, so as to reduce the space conflict between excitation objective and sample;Secondly, the collection objective is placed with a small inclination angle, and is matched with a liquid chamber with the same inclination angle with optical window, and the configuration determines the imaging depth of the collection objective;Then, the collection objective and imaging buffer solution matching the refractive index of the tissue transparent sample are selected, and the configuration can avoid optical aberration to realize high-resolution imaging.By the present application, high-resolution imaging and depth imaging can be realized, and the transverse range of the image can also be improved compared with the prior art, which provides strong support for the research of biological science and clinical medicine.

The utility model discloses a novel animal illumination intervention box, which relates to the technical field of biological science, and comprises an experiment box, the top of the experiment box is provided with an illumination mechanism, and the inner wall of the experiment box is provided with an exhaust fan; the illumination mechanism comprises a top cover, and one side of the top cover is fixedly connected with the experiment box. Through cooperation of the limiting plate, the LED lamp panel, the first signal line, the illumination controller, the master controller, the second signal line and the control panel, an experimenter operates the control panel to send out a control signal, the signal is input into the master controller through the second signal line, the signal is input into the illumination controller through the master controller, and the illumination controller is connected with the LED lamp panel. Signals in the illumination controller are input into the LED lamp panel through the first signal line, the LED lamp panel changes the illumination intensity and color according to the signals, meanwhile, an operator can control the LED lamp panel to be turned on and off in a timing mode through the control panel, different natural environments can be simulated, higher flexibility and adjustability are provided, and different experiment requirements are met.

The invention relates to a non-contact electrical stimulation and recording assembly designed for use in electrophysiological studies in cell culture laboratories carried out in the field of biological sciences, medicine and pharmacy and an operation method thereof. The fact that this assembly of the invention is able to perform a non-invasive stimulation and recording process increases the standardization and reliability of the studies to be performed. In addition, the cost of the electrical stimulation and recording assembly of the invention is also reduced.

The invention discloses a serum metabolism marker combination for diagnosing and classifying infectious endocarditis and a screening method thereof, and relates to the technical field of biological science. According to the method, a serum metabolism fingerprint spectrum is obtained through a matrix-assisted laser desorption / ionization mass spectrometry (MALDI MS) technology, and feature screening and model construction are carried out in combination with multiple machine learning models (including ridge regression, random forest, LASSO regression and the like). Through machine learning, five-fold cross validation is adopted to optimize model performance, and independent queue verification is utilized to evaluate generalization ability and overfitting risk. According to the technical scheme, rapid diagnosis and classification of IE can be achieved within 10 minutes, the pretreatment process is simple (an existing IE diagnosis method is complex in process and consumes 2-5 days), the requirements of clinical IE rapid screening and large-scale sample analysis can be met, and the method is suitable for industrial production. The detection flux is obviously improved; the cost is reduced.

The utility model discloses a biological science microorganism fermentation tank which comprises a fermentation tank body, the top of the fermentation tank body is rotatably connected with a rotating pipe through an opening, one side of the top of the fermentation tank body is fixedly connected with a sucking pump, the air inlet end of the sucking pump is communicated with an air inlet pipe, and the air outlet end of the sucking pump is communicated with an air outlet pipe. The utility model relates to the technical field of fermentation tanks. According to the bioscience microbial fermentation tank, oxygen is extracted by controlling an air inlet pipe on an air extracting pump, the oxygen is discharged to a rotating pipe through an air outlet pipe, the rotating pipe enters an air spraying pipe through a ventilation pipe, the oxygen is sprayed into hierarchical areas with different heights through a plurality of air outlet holes in the air spraying pipe, and meanwhile a motor drives a rotating rod to rotate; the rotating rod drives the rotating pipe to rotate through the gear, so that the rotating pipe drives the gas spraying pipe to annularly rotate to spray oxygen, and therefore, the oxygen is well and uniformly distributed in different areas, the oxygen distribution is more uniform, and the microbial fermentation effect is guaranteed.

The invention discloses a method for a super-resolution large-depth three-dimensional scanning light sheet microscope, and belongs to the technical field of fluorescence imaging. Exciting light is processed by a cylindrical lens, and a longer working distance of an exciting objective lens is obtained by utilizing secondary convergence of a generated initial light sheet, so that the space conflict between the exciting objective lens and a sample is reduced; secondly, the collecting objective lens is placed at a small inclination angle and is matched with a liquid chamber which has the same inclination angle and is provided with an optical window, and the configuration determines the imaging depth of the collecting objective lens; and then a collection objective lens and an imaging buffer solution which are matched with the refractive index of the tissue transparent sample are selected, and the configuration can avoid optical aberration and realize high-resolution imaging. According to the invention, both high-resolution imaging and deep imaging can be realized, the transverse range of imaging is improved compared with the prior art, and powerful support is provided for research of biological science and clinical medicine.