201 results about "Biological sciences" patented technology

An Internet server matches experts offering consulting services in the biological sciences or the like with potential customers of such consulting services. The server presents each expert with a Web interface through which the expert inputs his or her qualifications and times of availability. The qualifications are organized in a two-tier hierarchy of broad subject areas and sub-areas within each area. The times of availability are organized in terms of starting time of availability and duration of availability from that starting time. The server also presents each customer with a Web interface through which the customer inputs the qualification and times of availability sought. The qualifications and times are organized in the same fashion as for the expert. The customer can input multiple service requests, each with different qualifications and times of availability. The server uses the input data to match experts with customers. At first, the experts and the customers are anonymous to one another. Once either an expert or a customer chooses to purchase the record concerning a match, the record with contact information is released, and the expert and the customer can contact each other. The server provides other functions, such as allowing a physician to contact a manufacturer or a product, using only the brand name of that product; allowing companies to conduct surveys of physicians; and providing distance learning.

An Internet server matches experts offering consulting services in the biological sciences or the like with potential customers of such consulting services. The server presents each expert with a Web interface through which the expert inputs his or her qualifications and times of availability. The qualifications are organized in a two-tier hierarchy of broad subject areas and sub-areas within each area. The times of availability are organized in terms of starting time of availability and duration of availability from that starting time. The server also presents each customer with a Web interface through which the customer inputs the qualification and times of availability sought. The qualifications and times are organized in the same fashion as for the expert. The customer can input multiple service requests, each with different qualifications and times of availability. The server uses the input data to match experts with customers. At first, the experts and the customers are anonymous to one another. Once either an expert or a customer chooses to purchase the record concerning a match, the record with contact information is released, and the expert and the customer can contact each other. The server provides other functions, such as allowing a physician to contact a manufacturer or a product, using only the brand name of that product; allowing companies to conduct surveys of physicians; and providing distance learning.

The invention belongs to the technical field of nanometer biological materials, and particularly relates to rare earth-doped upconversion nanometer crystal-based fluorescent coding microspheres and a preparation method thereof. According to the principle that an upconversion material doped by different rare earth ions emits fluorescent light with different wavelengths under the excitation of an infrared light source, in the rare earth-doped upconversion nanometer crystal-based fluorescent coding microspheres, a strength ratio of the emitted fluorescent light with different wavelengths is usedas fluorescent codes, namely the number of the fluorescent codes is changed by changing the doping varieties and doping content of the rare earth ions in the process of synthesizing nanometer crystals or the fluorescent codes are regulated by controlling ratios of upconversion nanometer crystals of different varieties in a microsphere carrier material. By the fluorescent coding microspheres, the sensitivity and number of detection can be improved further, and a plurality of fluorescent report molecules with different colors can be used simultaneously. The coding microspheres has the wide application prospects in aspects of gene expression, mutual effect among proteins, the high-flux screening of biological samples, the detection of multichannel biological science, disease diagnosis, combined chemistry and the like.

In order to solve the high-speed cancer excited state problem, a biological retarder is needed. Cancer energy is converted into intramolecular transfer or other vibrational state transfer through collision. By means of the nucleus retarder principle, a neutron and a retarder atomic nucleus are subjected to collision so that the speed can be decreased, and the retarder which does not absorb neutrons and does not react with neutrons is selected. An important task of the cancer biology and even the natural science is that which material can bear a cancer nuclear fission biological retarder. According to repeated experiments, various safety factors are synthesized, and finally a biological moderator is correctly selected through a successful experiment. Bifidobacterium is free of toxicity and harm to a body, and the biological retarder can be ideal for treating a cancer. In the future, more microorganism retarders will be produced, the biological retarder is used for decelerating tumors, a proper amount of deceleration molecular nuclear energy can be used as senile cell addition nuclear energy, and the biological retarder gives power to biological science and will play an indispensable critical role.

The present invention relates to devices and chips comprising large scale chemical field effect transistor arrays including an array of sample retention regions capable of retaining a chemical or biological sample from a sample fluid for analysis. In one aspect, such an array of transistors has a pitch of 10 μm or less, and each sample retention region is located on at least one chemical field effect transistor arranged to generate at least one output signal, which signal is consistent with such related to the characteristics of the chemical or biological sample in the sample retention area. In one embodiment, said chemical or biological sample is characterized by the concentration of charged species, and wherein each of said chemical field effect transistors is an ion-sensitive field effect transistor having a floating The dielectric layer is in contact with the sample fluid and is capable of accumulating charge in proportion to the concentration of charged species in the sample fluid. In one embodiment, such charged species are hydrogen ions, whereby the sensor measures the change in pH of the sample fluid in or near its sample retention zone. The devices and chips of the present invention may be suitable for large-scale pH-based DNA sequencing and other bioscience and biomedical applications.

Biotic fertilizers are described that build soil nutrients by accelerating the growth of topsoil microorganisms Biotic fertilizers are primarily aimed at increasing populations of cyanobacteria, formally known as blue-green algae, and like organisms that have the ability to engage in photosynthesis reand to engage in the extraction of nitrogen from the atmosphere. Methods of biotic fertilizer manufacture are presented that utilize animal waste product as well as desirable compositions obtained thereby.

The invention belongs to the technical field of bioscience and medical science health-care foods and relates to a formula and preparation method of a ginseng health-care cake containing eight delicacies. The ginseng health-care cake containing eight delicacies is obtained by formulating and processing Chinese medicines including ginseng, coix seeds, hindu lotus lumule, gordon euryale seeds, Chinese yam, tuckahoe, atractylis ovata, semen lablab album and the like together with food rice powder (or glutinous rice flour or flour), white sugar (or gooey or xylitan), osmanthus and the like by using a biotechnology, medical science theory and a health-care food formula preparing technology, is rich in nutrition, has the effects of resolving phlegm, detoxicating, destroying intestinal worms, clearing liver, improving vision, reinforcing the spleen, arresting diarrhea, enriching skin, polishing, effectively improving blood circulation of blood vessels of the brain and peripheral vessels, treating arteriosclerosis, hypertension, diabetes and senile dementia, lowering serum and cholesterin, resisting ageing, nodule and fungi, preventing the growth and the diffusion of cancer cells, invigorating the brain, strengthening the body, keeping fit, beatifying and preventing and treating diseases, and is a safe, nutritional and healthy food for people to eat.

The invention discloses a special spectrum simulation illumination device for plant cultivation and a simulation method. In the invention, the design idea of single light source is broken through; a hexagonal extensible light source array is designed based on LED combination; control power supply is realized by signal chip computer control and inductance switch stabilized current; the configuration parameter of a light source is determined by a monochrome calibration method; and the simulation of a special spectrum is realized by a nonlinear least squares curve fitting. The device has the advantages of special spectrum simulation, light-reliable-efficient performances, convenient extension and strong commonality. The simulation test result shows that the invention can pointedly and flexibly simulate the visible light absorption spectrum of special plants, solves the technical problem that how to supply light efficiently and flexibly for biological culture and provides a new technical solution for biological scientific study, biological culture and fresh-keeping in special environment and the like.

The invention relates to the field of chemical and biological sciences, in particular to LQC-X structural formula I, and synthesis and application of intermediate of LQC-X, wherein a maximum dosage of LQC-X6 given to an mouse is 2000 mg / kg once in one day; the mouse is continuously observed for 7 days; no toxic reaction appears, and the medicine is proven to have quite high safety; and it is proved through pharmacodynamics experiments that LQC-X compounds have obvious anti-hepatitis B virus action and liver protecting and transaminase lowering action. The compounds can be used for preparing medicines for preventing and treating diseases of hepatitis B, chronic liver cirrhosis and the like. The structural formula I of LQC-X is shown in the description.

This invention relates to one enzyme and puffing weed fiber pastry paper processing method, which combines biological science, life science and mechanic process to realize paper process without dirty water discharge. The method is characterized by the following: through grinding for clutter and mixture removing and cutting into short weed fiber materials into press machine; adding H2O2 solution and fiber enzyme B and induce agent C for pressing then for enzyme transmission and sending into puffing machine to get rough pastry.

The invention provides a method for selecting and breeding a novel silkworm breed capable of producing a great number of all natural pure sericin proteins, which introduces a special germ plasm resource from Japan, adopts genetic thremmatology to be combined with bioscience, and utilizes a distant hybridization method to breed a silkworm breed with sturdy, disease prevention, high temperature resistance, short breeding period and excellent economic character. The method leads excellent genes of the silkworm breed in the germ plasm resource to breed into an all natural pure sericin silkworm breed with high purity, and provides a novel approach for non spun silk industries to produce all natural pure siricin materials.

The present invention discloses a near-infrared hydrazine ratio fluorescence probe, and the probe is dicoumarol-containing 4 - (2 '- carboxyl phenyl) - 2 - (7' - diethylamine coumarin) - 7 - diethylamino benzo pyran perchlorate with the chemical structural formula shown in formula (I). The probe can selectively effect with N2H4 to change from green to yellow, can be distinguished by the naked eye, fluorescence changes from red to blue-green, and fluorescence ratio response effect is very good, and can also be analyzed by UV absorption and fluorescence spectrometry. N2H4 ratio detecting can be achieved, intracellular ratio imaging can be achieved, and the content of the gaseous N2H4 can be quickly detected by the naked eye by use of a prepared silica gel plate. The probe is expected to play a role in industrial production and biological sciences, and has broad application prospects.

The invention discloses a ratiometric fluorescent probe for detecting bisulfite, which is obtained by taking dansulfonyl fluorophore as a donor, taking (E)-2-(3-cyano-4-(4-(dimethylamino) styrene)-5,5-dimethylfuran-2(5H)-subunit)malononitrile fluorophore as a receptor, using piperazine linking and basing on a fluorescence resonance energy transfer mechanism. The invention also discloses application of the ratiometric fluorescent probe in detection of a sample containing the bisulfite and positioning imaging of intracellular lipid droplets. The ratiometric fluorescent probe provides a powerfultool for correlated detection or targeting localization in the field of biomedicine, is expected to play a role in biological sciences research and has a broad application prospect.

The invention discloses a streptomyces virginiae IBL14 type I-B-sv14 type CAS gene editing system for the first time. The system comprises establishment of protein expression plasmid and gene editing plasmid, and gene edition and immunization process of escherichia coli and bacillus subtilis. The system can perform gene edition and immunization on prokaryotic genomes conveniently, quickly and effectively. The gene editing and immunizing method can be widely applied to the fields of biological science, pharmacy, food, agriculture and other application.

The invention discloses a short peptide and application thereof, belonging to the fields of biological science and medicinal carriers. The short peptide consists of a cationic cell penetrating peptide connecting sequence and a targeted peptide sequence, and the amino acid residue sequence of the short peptide is shown as SEQ ID NO.1 in a sequence table. The short peptide can be used for targeted transfection of tumor cells.

The invention discloses a polypeptide and nanometer particles thereof for carrying antigen peptides, preparation methods and applications thereof, and belongs to the field of biological science and drug carriers. The polypeptide is formed by series connection of an alpha-helix polypeptide, a connecting sequence and an antigen peptide in a covalent bond manner, wherein the amino acid sequence of the alpha-helix polypeptide is FAEKFKEAVKDYFAKFWD, the amino acid sequence of the connecting sequence is GSG, and the connecting sequence of the antigen peptide is KVPRNQDWL. The polypeptide and the nanometer particles prepared from the polypeptides are capable of effectively improving the tumor antigen peptide uptake efficiency of the dendritic cells and promoting the prevention and treatment effect of tumors after the DC (dendritic cell) takes in the antigen peptides.

The invention specifically provides a foodstuff, medical formulated foodstuff, specific medical formulated foodstuff, health-care product and drug for patients with disorders in intestinal microecology, belonging to the technical field of both medicinal and edible prescriptions and new resource food. Prescriptions for intestinal-microecology regulating preparations in the invention are developed according to the physical characteristics of people with disorders in intestinal microecology and on the basis of reasonable combination of quintessential theories of traditional Chinese medicine and modern bioscientific progress. Specifically, the intestinal-microecology regulating preparations are prepared from probiotic powder obtained after vacuum freeze drying and adjuvants consisting of prebiotics, cereal fiber and multiple natural plant essences of medicinal and edible traditional Chinese medicines and the like through mixing, granulation and other processes, and are capable of forming probiotic intestinal barriers, promoting spleen yang, invigorating the spleen, regulating middle energizer, preventing inflammations, relieving pain and eliminating dampness and heat. The intestinal-microecology regulating preparations can effectively improve gastrointestinal diseases like diarrhea, enteritis and dyspepsia caused by disorders in intestinal microecology; the preparations can improve insomnia dreaminess due to the tranquillizing effect of ginseng; and kudzu vine roots and turmeric in the preparations can relieve or neutralize the effect of alcohol, protect the liver, and alleviate discomfort caused by alcohol.

The invention discloses a polypeptide carrying melittin, nano-particles carrying melittin, and applications thereof. The invention belongs to the field of biological science and drug carriers. The peptide is composed of alpha helical polypeptide, connection sequence, and melittin which are connected in series with a covalent bond form. The nano-particles are formed through three effective bonding manners of polypeptides, phospholipids, and cholesterol esters carrying melittin. With the nano-particles prepared from the polypeptide carrying melittin, toxic and side effects of melittin against bodies can be effectively reduced, and effects can be performed specifically at diseased areas such as tumors. The nano-particles can be used in clinical treatments.

The molecules harbored in exosomes play important roles in biological science. A highly desirable goal for exosome research is the rapid, simple, simultaneous tracking and quantification of exosome harbored molecules. Disclosed herein are methods and devices for inducing the release and measurement of biomolecules harbored in exosomes. The disclosed method, Electric Field Induced Release and Measurement (EFIRM) technique, uses an electrical field to simultaneously disrupt exosomes to release the contents and measure the harbored exosomal RNA / proteins. The exosome vesicle contents can be released within minutes. This provides a potential on-site method for the detection of exosome-harbored biomolecules.

The invention discloses a gene marker for liver cancer detection and application thereof, and the gene marker is especially suitable for detection of early liver cancer and / or detection of AFP-negative liver cancer, and the application field is biological science comprising biology, biochemistry, biotechnology, medicine and medical technology. The gene marker is composed of an EP400 gene, a MAPK1IP1L gene, a NUFIP2 gene, a PHC3 gene, a RPS6KB1 gene and a STX7 gene. The gene marker disclosed by the invention has the advantages that when the gene marker is used in liver cancer screening diagnosis, the sensitivity is high, and the specificity is strong, due to the fact that peripheral blood which is clinically most easily collected is used as a detection sample, the detection is convenient, and the gene marker is suitable for early diagnosis of liver cancer and detection of an AFP negative liver cancer patient, and has a wide application prospect.

The invention provides a pin-column typemini-size radiator having an end face with a phyllotaxy structure. The invention belongs to the technical field of mini-size raditors used for heat dissipation of electronic components and relates to the design of distribution structure of radiating columns of the radiator with the phyllotaxy structure. The radiator includes a radiation bottom board and the multiple radiation columns arranged on a working end face of the radiation bottom board. The distribution of the radiation of the mini-size radiator meets an H. Vogel model employing a phyllotaxy theory in biological science. Since the radiation columns are in distribution meeting the H. Vogel model law on the surface of the bottom board of the radiator, geometric position compensation and maximum filling effect are realized by the radiation columns on the working surface of the mini-size radiator.

The invention relates to the field of the biological science, in particular to a diagnostic kit and an application of coiled-coil domain containing protein 49 (CCDC49) in preparing a gastric cancer early-stage diagnostic reagent. The diagnostic kit is used for diagnosing the early-stage gastric cancer. The diagnostic kit comprises an elisa plate, human protein CCDC49, standard serum, an enzyme labeled reagent, enzyme substrate solution, confining liquid, sample diluent, detergent liquid and termination liquid, wherein the human protein CCDC49 is wrapped on the elisa plate. Compared with the prior art, the diagnostic kit has advantages that 1, a commodity diagnostic kit which is sensitive, safe, reliable and easy to operate is provided, the immunoglobulin m (IgM) antibody for resisting the protein CCDC49 in the human serum can be qualitatively determined, and the early diagnosis of the gastric cancer can be assisted; and 2, the specificity of the provided serum biological labeled protein CCDC49 is 81 percent, the sensitivity is 82 percent, and the characteristics of high specificity and high sensitivity can be realized.

The invention provides a beautifying noodle for nourishing and activating skin cells which is prepared from following materials in weight ratio: wheat flour 83-95%, functional materials 15%, deer velvet 1%, ass-hide glue 1%, spatholobus root and vine 1%, ginseng root 1%, lance asiabell root 1%, astragalus root 1%, fleeceflower root 1%, udo root, prepared rehmannia root 1%, atractylodes rhizome 1%, Indian bread 1%, danshen root 1%, citrus 1%, Chinese wolfberry fruit 1%. The production method of the inventive product includes the steps of checking material , drying materials, grinding materials into powder, mixing, preparing biological liquor, bio-fermenting, debitterizing ,mixing materials, compacting noodles, drying and packaging. The inventive product is prepared for containing total nutrition of materials according to Chinese food nourishing theory, modern biological sciences and traditional Chinese medicine theory without using any harmful chemical substance as additive, keeps original juice, flavor and color of materials and remains unpolluted and unharmful, and is endowed with bioactivity due to beneficial microbe by adopting biocontrol technology, biological fresh keeping technology.

The invention discloses recombinant neutrophil gelatinase associated lipocalin and a preparation method thereof. The preparation method of the recombinant neutrophil gelatinase associated lipocalin antibody belonging to the technical fields of biological science and transthyretin comprises the following steps: analyzing an NGAL (neutrophil gelatinase associated lipocalin) epitope; synthesizing the recombinant NGAL; separately purifying the recombinant NGAL; preparing an anti-rabbit recombinant NGAL antibody; collecting and separating to obtain an antiserum purified antibody containing the antibody, so as to obtain the anti-NGAL antibody. By adopting the preparation method, the problem of low yield in preparation of a multi-antibody by a natural full-length protein is solved; meanwhile, the antibody prepared by the preparation technology disclosed by the invention has good specificity; the problem that the traditional neutrophil gelatinase associated lipocalin antibody cannot completely meet a western blot immunohistochemical experiment and an enzyme-linked immunosorbent assay is also solved.

The invention discloses a method for separating enriched target cells from tissues, and belongs to the field of biological science and medicament development. The method comprises the following steps of: preparing the tissues into suspension of single cells, and separating and enriching target cells from the cell suspension by using a positive enrichment method. The method can separate and enrich the target cells from the tissues in different treatment states, and provides enough and high-purity experimental materials for basic research, clinical research, diagnosis, medicament development and the like.

The invention provides a method for extracting and purifying anthocyanin from bilberry. The method comprises the following steps: (1) microbial degradation; (2) obtainment of a crude extract of bilberry anthocyanin by utilizing a water extraction process; (3) addition of a certain amount of a flocculant for impurity removal; (4) impurity removal and purification with macroporous resin; (5) concentration; and (6) spray drying. According to the invention, by combining aqueous-solution extraction and microbial degradation, an acylated stable structure of anthocyanin is realized through microbial degradation; in addition, the juice yield of bilberry is greatly improved through cellulase hydrolysis; thus, double purposes are achieved through one way, and interdisciplinary penetration and effective combination of biological science and chemical production are realized. The method provided by the invention also utilizes ZTC1 + 1 for flocculation and impurity removal, and solves the problems of easy loss of active ingredients and long production cycle in traditional water extraction and alcohol precipitation. The extraction method provided by the invention has the advantages of simple process operation, low production cost, no pollution, high extraction rate, and no damage to anthocyanin due to high temperature; and the anthocyanin prepared by using the method provided by the invention can be extensively applied to fields like food, health-care products and cosmetics, and has extremely high application values.

The invention provides a fluorescence probe ZY9 for visually detecting hydrazine based on benzothiazole as well as a preparation method and use of the fluorescence probe ZY9. The fluorescence probe ZY9 can be prepared through the reaction of 2-(4-aminophenyl)benzothiazole and phthalic anhydride in a certain proportion and is easy to synthesize and purify, relatively high in yield and low in cost;the hydrazine fluorescence probe is a fluorescence enhanced fluorescence probe, has relatively selectivity and sensitivity to hydrazine, is strong in anti-jamming capability and high in response speedand can be used for visually detecting hydrazine through the significant change of the fluorescence color; the probe is good in stability and can be stored for a long time for use; besides, the probeis suitable for detecting the content of hydrazine and realizing the fluorescence imaging of hydrazine in a living cell level, and hydrazine in a water solution with a certain concentration can be detected by virtue of probe test paper. A hydrazine detection method provided by the invention has the advantages of easiness and convenience in operation, easiness in popularization and the like and has wide application prospects in the fields of environment and biological science.

The invention relates to a method, a device and a system for optimizing performance of a cardiac valve prosthesis. The method comprises steps as follows: a cardiac valve image acquired through a scanning device is obtained; an aorta section model is extracted from the cardiac valve image; a primary valve model is obtained through mesh optimization; an artery is established, and an artery blood model is obtained; a solid valve model and a solid arterial wall model are obtained through model optimization; a cardiac valve prosthesis design result is obtained through finite element analysis. A finite element method is combined with biological science, and the difference between the cardiac valve prosthesis and a natural cardiac valve is narrowed by using a real body scan image to re-establish a biovalve model. The re-established model is subjected to materialized processing, key features of valve appearance are extracted, the model is subjected to secondary reestablishment, and a three-dimensional model closer to the reality is obtained. The model can present the appearance of the cardiac valve prosthesis really and can also perform finite element analysis on the stress distribution of the valve impacted by blood, and accordingly, the performance of the cardiac valve prosthesis is improved.

The present invention belongs to the fields of the biolobical science and the biotechnology, and relates to a method for abstracting, edulcorating, cultivating and optimizing the symbiotic blue green algae. Separation and purification includes the steps that after the surface sterilization, the immersion, the sterile water cleansing, the glass homogenizer grinding, the centrifuge of filter liquor, the deposition and the re-suspending, a symbiont is cultivated with culture solution containing nitrogen BG-11 (N) in the shaking way in the initiating stage, and cultivated with the culture solution containing non-nitrogen BG-11 (N) in the late stage, at the same time, pure colony is abstracted through marking on the flat plate of the BG-11 (N) solid cultivation medium until being edulcorated. Cultivating and optimizing is provided with the conditions that the intensity of illumination is 160 micromole . m - 2 . s -1 , the illumination cycle is 18 h / 6 h, the pH value is 8.4, the temperature is 25 DEG C, and the humidity is 90 percent. The present invention has the advantages that the abstraction and the edulcoration of the symbiotic blue green algae is time saving and labor saving, the vitro nutrition mode of the symbiotic blue green algae can be established rapidly, the very pure symbiotic blue green algae can be obtained, and thus the basis is founded in order to further research the essence of the symbiotic blue green algae and the application of the active metabolic metabolites of the symbiont.

The invention belongs to the technical field of chemical substance detection and relates to a differential confocal discrete fluorescence spectrum and fluorescence lifetime detection method and device. The basic thought is that a differential confocal object surface positioning technology with a precision axial resolution ratio and a discrete fluorescence spectrum and fluorescence lifetime measurement technology are fused; high-precision measurement of a three-dimensional shape of the surface of a sample to be detected is solved by utilizing a differential confocal technology; meanwhile, high-sensitivity detection of a fluorescence spectrum and fluorescence lifetime of each point on the surface of the sample to be detected is solved by utilizing the discrete fluorescence spectrum and fluorescence lifetime measurement technology, so that three-dimensional high-resolution space substance component distribution information is obtained. According to the differential confocal discrete fluorescence spectrum and fluorescence lifetime detection method and device, a differential confocal measurement technology and a fluorescence substance component detection technology are fused for the first time; a fluorescence imaging system has the same transverse resolution ratio on each position of the surface of the sample to be detected; finally, measured fluorescence spectrum distribution accurately corresponds to the three-dimensional shape. The technology provided by the invention has a wide application prospect in the fields of biological science, medical science, material science and clinical medical diagnosis.