23 results about "Biological sciences" patented technology

The application discloses a seminal plasma metabolic biomarker combination for male infertility spermatogenesis obstacle typing diagnosis, and relates to the field of biological science and technology.The application records the metabolic fingerprints of 475 seminal plasma samples by means of nanoparticle-enhanced laser desorption / ionization mass spectrometry, and constructs a seminal plasma metabolic fingerprint database related to spermatogenesis obstacle (SPGF). Then, through machine learning and statistical analysis, six metabolic biomarkers for SPGF typing diagnosis are screened and identified in the seminal plasma, and the six metabolic biomarkers can realize accurate typing of SPGF (AUC=0.814). The application has the advantages of simple sample pretreatment, fast analysis speed and low cost by means of recording the metabolic fingerprints of seminal plasma by means of nanoparticle-enhanced laser desorption / ionization mass spectrometry. Through the synthesized iron oxide nanoparticles as a matrix, small molecule metabolites in the seminal plasma can be efficiently captured and ionized, and complex pretreatment steps such as deproteinization and chromatographic separation are not needed, so that super-fast analysis, super-high sensitivity, extremely low sample consumption and excellent repeatability detection are realized.

The invention belongs to the technical field of fluorescent probes, and particularly relates to a fluorescent probe for detecting pyrophosphoric acid or pyrophosphate based on ratiometric fluorescence, a preparation method and application. According to the fluorescent probe, cyclotriphosphazene is taken as a core platform, a naphthalimide-triphenylamine luminophore and a terpyridine recognition unit are introduced, a multi-arm fluorescent probe is integrally formed, coordination terpyridine interacts with metal ions Zn < 2 + >, fluorescence signals are induced to change, and therefore sensitive detection on PPi is achieved. The fluorescent probe has the advantages of quick response, high selectivity, interference resistance and low cost, and has important application value in the fields of environmental science, biological science and the like.

The utility model relates to the technical field of biological science experimental equipment, in particular to sample preservation equipment for IV-type collagen detection, which comprises a base, a box body is rotatably connected to the inner wall of the base, a box cover is in threaded connection with the surface of the box body, an inner box is rotatably connected to the inner wall of the box body, and the inner box is in threaded connection with the box cover. The lower end of the inner box is fixedly connected with the inner wall of the base, two symmetrically-arranged through grooves are formed in the surface of the inner box, two symmetrically-arranged sliding rods are fixedly connected to the surface of the inner box, and two symmetrically-arranged sliding grooves are formed in the inner wall of the box body. According to the sample tube fixing device, the sample tubes are effectively fixed, the sample tubes are tightly clamped by the fan-shaped blocks in the closed state of the device, the sample tubes are prevented from falling off due to shaking or external force, the stability of the sample tubes in the storage process is guaranteed through the fixing mode, and damage caused by mutual collision of the sample tubes is avoided.

This invention belongs to the interdisciplinary field of bioscience and information technology, and relates to a method for assessing the intensity of gene transcriptional regulation based on multi-omics data. Addressing the challenges of systematically integrating multi-omics data and quantitatively characterizing the correspondence between regulatory elements and genes, as well as the intensity of regulation, in existing transcriptional regulation analyses, this invention integrates epigenomics, transcriptomics, and three-dimensional genomics data to construct a site-gene regulatory intensity model. This method extracts candidate cis-regulatory sites and calculates their regulatory activities. It then determines the site-gene regulatory weights by combining distance weighting and three-dimensional genomic contact information, thereby obtaining the regulatory intensity at the site-gene pair and gene levels. An iterative algorithm distinguishes between positive and negative regulatory effects, achieving correction and summarization of regulatory intensity. This invention can quantitatively assess the regulatory intensity of cis-regulatory elements on target genes and the overall regulatory effect on genes, and is suitable for studying gene transcriptional regulation mechanisms and analyzing gene expression changes under different treatment conditions.

1. The name of the design product: cell detector (handheld). 2. The use of the design product: the design product is used for biological science detection and analysis. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective view.

The utility model relates to the technical field of biological science, in particular to a spiral microbial reactor, which comprises moving seats, locking devices, fixing devices, inserting devices and bent pipes, straight pipes are fixed by the fixing devices, then the straight pipes move on a support plate through a plurality of moving seats and are locked by the locking devices, and the inserting devices are inserted into the straight pipes. The distance between every two adjacent straight pipes is equal to the distance between the two ends of the corresponding bent pipe, finally, the bent pipes and the straight pipes are connected through the inserting devices, the spiral pipe reactor can be installed, and compared with the prior art, installation of a plurality of pipelines can be completed.

The invention discloses a serum metabolism marker combination for ectopic pregnancy diagnosis and rupture risk prediction and application of the serum metabolism marker combination, and relates to the technical field of biological science. The metabolic fingerprint diagnosis model (AUCgt, 0.91) and the metabolic marker panel (AUCgt, 0.92) have the advantages that the performance of the metabolic fingerprint diagnosis model (AUCgt, 0.91) and the performance of the metabolic marker panel (AUCgt, 0.92) are obviously superior to those of an existing'ultrasonic + beta-hCG 'diagnosis mode, the problems of diagnosis delay, misdiagnosis and missed diagnosis clinically at present are effectively solved, and the clinical rigid requirements of early and accurate diagnosis are met; the fracture risk prediction model AUC 0.885 constructed by the method disclosed by the invention fills the current clinical blank. Key decision support can be provided for doctors, early intervention on high-risk patients is achieved, excessive medical treatment on low-risk patients is avoided, and the mortality risk of pregnant and lying-in women is reduced. According to the invention, based on the nanoparticle-assisted laser desorption / ionization mass spectrometry technology, ultra-fast detection of only about 3 seconds for single sample detection is realized, and the method is expected to become a fast detection tool in emergency treatment and outpatient service scenes.

The invention discloses a plasma metabolism biomarker combination for predicting and intervening propofol-induced respiratory depression, a detection method and application, and relates to the technical field of biological science. According to the method, non-target metabonomics detection is conducted on preoperative plasma of 298 subjects through UPLC-MS, 14-metabolite panels are obtained through machine learning supervised feature screening, a diagnosis model is established through verification of discovery and verification queues, and the prediction AUC of severe respiratory depression reaches 0.86-0.91 (discovery queue) and 0.86-0.91 (verification queue). Mouse in-vivo function verification proves that the key metabolite alpha-linolenic acid obviously improves the respiratory rate, the tidal volume and the minute ventilation volume under two administration paradigms of pretreatment and treatment of a mouse propofol-RD model, shortens the recovery time of righting reflex, has a good safety foundation, and has a good application prospect. Prompt can be converted into a foresight supplement or rapid treatment medication thought in a perioperative period, and a'prediction and intervention 'integrated clinical value is formed.

The invention discloses a DNA methylation recognition method based on deep learning and multi-scale feature fusion, and belongs to the crossing field of artificial intelligence and bioscience, the method comprises the following steps: obtaining original data of a DNA sequence to be detected, and carrying out preprocessing and feature extraction on the original data to obtain a time-frequency feature matrix; inputting the time-frequency feature matrix into a pre-trained multi-scale feature fusion network, wherein the multi-scale feature fusion network performs classification through RepVGG modules with different expansion rates arranged in parallel, 1 * 1 convolution and a full-connection network to obtain a classification result; and determining the methylation state of the DNA sequence based on the classification result.

The utility model discloses a glass slide arrangement frame, which belongs to the technical field of biological science and comprises a box body, a fixing frame is fixedly connected onto the inner wall of the box body, a double-shaft motor is fixedly connected onto the fixing frame, two output shafts of the double-shaft motor are both fixedly connected with a right-hand threaded column and a left-hand threaded column, and the right-hand threaded column and the left-hand threaded column are fixedly connected onto the left-hand threaded column. And one end of the right-hand threaded column and one end of the left-hand threaded column are both fixedly connected with rotating shafts, one ends of the two rotating shafts are both sleeved with first bearings, and the two first bearings are both fixedly connected to the inner wall of the box body. The device has the beneficial effects that by arranging a rotating plate, a first threaded column and a threaded hole, the rotating plate drives the first threaded column to rotate in the threaded hole by rotating the rotating plate, so that a limiting rod moves in a limiting hole, and the first threaded column drives a U-shaped movable seat to get close to a corresponding U-shaped placement groove; the glass slide can be conveniently limited and fixed through the U-shaped movable seat and the U-shaped placing groove, so that the glass slide is kept stable.

The present application relates to the field of biological science and probiotic engineering, and particularly relates to a non-inducible promoter applied to probiotics and application thereof, the promoter can realize stable and efficient expression of a target gene in probiotics, preferably EcN1917, without any external induction operation, thereby significantly improving the functional characteristics of EcN1917 as probiotics.

The application discloses a kind of biological scientific research data collaborative analysis system based on cloud platform, it is related to data analysis technical field, including management center, the management center is connected with basic acquisition module, platform processing module, automation analysis module and service support module;To biological scientific research data data transmission change, obtain biological scientific research signal, to biological scientific research signal is dimension conversion, obtains biological wave variation array;Through updating prediction array, biological wave variation array is replaced with number and number chart conversion, obtains the graph of biological science, sets up mobile capture axis and obtains identity characteristic section to the graph of biological science;According to position node interval, identity characteristic section is distinguished by type, obtains biological identity type library, filters combination to biological identity type library, obtains biological type sequence;Efficient data processing is carried out using cloud platform, optimization collaboration experience is ensured Data security.

The present application relates to the field of biological science, and in particular to a method for assembling high-quality goose genome sequence using multiple sequencing technology means. The method uses Pacific Biosciences (PacBio) HiFi reading, Ont nanopore super-long reading, Illumina short reading and chromatin conformation capture (Hi-C) multiple sequencing technology means, and successfully assembles high-quality goose genome sequence. The assembled goose T2T chromosome level genome lays an important research foundation for future genetic improvement and genetic mechanism analysis of geese.

The application discloses a kind of methods of super-resolution large depth three-dimensional scanning light sheet microscope, belong to fluorescence imaging technical field, the excitation light is handled by column lens in the present application, the longer excitation objective working distance is obtained using the secondary convergence of the initial light sheet generated, so as to reduce the space conflict between excitation objective and sample;Secondly, the collection objective is placed with a small inclination angle, and is matched with a liquid chamber with the same inclination angle with optical window, and the configuration determines the imaging depth of the collection objective;Then, the collection objective and imaging buffer solution matching the refractive index of the tissue transparent sample are selected, and the configuration can avoid optical aberration to realize high-resolution imaging.By the present application, high-resolution imaging and depth imaging can be realized, and the transverse range of the image can also be improved compared with the prior art, which provides strong support for the research of biological science and clinical medicine.

The invention relates to a non-contact electrical stimulation and recording assembly designed for use in electrophysiological studies in cell culture laboratories carried out in the field of biological sciences, medicine and pharmacy and an operation method thereof. The fact that this assembly of the invention is able to perform a non-invasive stimulation and recording process increases the standardization and reliability of the studies to be performed. In addition, the cost of the electrical stimulation and recording assembly of the invention is also reduced.

The invention discloses a serum metabolism marker combination for diagnosing and classifying infectious endocarditis and a screening method thereof, and relates to the technical field of biological science. According to the method, a serum metabolism fingerprint spectrum is obtained through a matrix-assisted laser desorption / ionization mass spectrometry (MALDI MS) technology, and feature screening and model construction are carried out in combination with multiple machine learning models (including ridge regression, random forest, LASSO regression and the like). Through machine learning, five-fold cross validation is adopted to optimize model performance, and independent queue verification is utilized to evaluate generalization ability and overfitting risk. According to the technical scheme, rapid diagnosis and classification of IE can be achieved within 10 minutes, the pretreatment process is simple (an existing IE diagnosis method is complex in process and consumes 2-5 days), the requirements of clinical IE rapid screening and large-scale sample analysis can be met, and the method is suitable for industrial production. The detection flux is obviously improved; the cost is reduced.

The invention discloses a method for a super-resolution large-depth three-dimensional scanning light sheet microscope, and belongs to the technical field of fluorescence imaging. Exciting light is processed by a cylindrical lens, and a longer working distance of an exciting objective lens is obtained by utilizing secondary convergence of a generated initial light sheet, so that the space conflict between the exciting objective lens and a sample is reduced; secondly, the collecting objective lens is placed at a small inclination angle and is matched with a liquid chamber which has the same inclination angle and is provided with an optical window, and the configuration determines the imaging depth of the collecting objective lens; and then a collection objective lens and an imaging buffer solution which are matched with the refractive index of the tissue transparent sample are selected, and the configuration can avoid optical aberration and realize high-resolution imaging. According to the invention, both high-resolution imaging and deep imaging can be realized, the transverse range of imaging is improved compared with the prior art, and powerful support is provided for research of biological science and clinical medicine.

The utility model aims to provide a storage device for biological science test tubes, and relates to the technical field of biological experiment instruments, a plurality of placing holes are linearly formed in a box body, and movable clamping rods and fixed clamping plates are symmetrically mounted in the placing holes; the test tubes in the placing holes can be preliminarily clamped under the action of the elastic telescopic rods of the fixed clamping plate and the movable clamping plate; a rotating shaft is driven to rotate by rotating a sealing plate installed on a box body in a closed mode, a driving transmission assembly controls a movable clamping rod and an elastic telescopic rod to slide, and a clamping plate moves towards a fixed clamping plate, so that test tubes are clamped, and the test tubes are prevented from being collided and damaged in the transporting and moving process; when the test tubes are taken, the control pieces corresponding to the placement holes in the box body are rotated, so that the clamping plates loosen the clamped test tubes, and the test tubes fall into the sliding-out channel, pass through the collection channel and slide to the taking-out opening, so that the test tubes can be taken without opening the sealing plate above, and the probability that the test tubes are polluted is reduced.

The present application relates to material and detection technology, disclose a kind of fluorescent molecular material and opening type fluorescent probe, and it is applied to detect phenylthiol.The fluorescent probe is naphthalimide-triphenylamine as fluorophore, 2,4-dinitrobenzene as recognition group, is a kind of high sensitivity and selective water-soluble fluorescent probe, for the selective detection of phenylthiol.The probe has significant sensitivity and specificity to almost pure aqueous solution of phenylthiol, can realize the qualitative, quantitative detection or visual rapid detection of phenylthiol in actual sample, has good development use prospect, has wide application prospect in the sensing of environment and bioscience.

The application belongs to the field of biological science and biotechnology, and particularly relates to application of a specific target sgRNA for knocking out a SIRT7 gene and a method for constructing a streptococcus-resistant and low-temperature-resistant tilapia strain. The SIRT7 gene of the tilapia is knocked out through a CRISP / Cas9 gene editing technology, and the obtained SIRT7 gene deletion tilapia has stronger streptococcus resistance and low-temperature resistance, and the excellent germplasm of the streptococcus-resistant and low-temperature-resistant tilapia is obtained, which provides a new target for the research on the disease resistance and low-temperature resistance of farmed fish.

The invention relates to the technical field of biological science, and discloses a plant lactobacillus microencapsulated preparation and application of the plant lactobacillus microencapsulated preparation in preparation of a hypoglycemic product, and the plant lactobacillus microencapsulated preparation is prepared from plant lactobacillus XKFY202518. The preservation number of the plant lactobacillus XKFY202518 in the China General Microbiological Culture Collection Center (CGMCC) is CGMCC No.34745, and the preservation date of the plant lactobacillus XKFY202518 is June 3, 2025. According to the invention, the plant lactobacillus microencapsulated preparation is compounded with the white kidney bean extract, the mulberry leaf extract and the prebiotics to form a triple synergistic system of probiotic metabolism regulation, enzyme inhibitor targeted sugar control and prebiotics colonization promotion. All the components are good in compatibility, no effect antagonism phenomenon exists, not only can different sugar control links such as amylolysis and disaccharide absorption be covered, but also intestinal colonization of probiotics can be promoted, and the overall effects of reducing blood sugar and improving lipid metabolism are remarkably improved.

The invention discloses a high school biological science experiment cell culture device, which comprises a cylindrical shell, the top wall of the cylindrical shell is a transparent cover plate, students can conveniently observe conditions in a box without opening the device, the transparent cover plate is provided with a fan-shaped opening, the fan-shaped opening is provided with a sealing cover plate capable of sliding along the transparent cover plate, and the sealing cover plate can slide along the transparent cover plate. When the cylindrical shell is closed, the sealing performance of the culture cavity can be guaranteed, a rotatable bearing plate which is horizontally arranged is arranged in the cylindrical shell, a rotating shaft is rotationally arranged at the center of the cylindrical shell, and the rotating shaft is fixedly connected with the center of the bearing plate. The invention belongs to the technical field of biological experiment equipment, and particularly provides an integrated rotating and lifting mechanism, which realizes rapid and accurate taking of specific culture dishes under the condition of minimum opening of a cabin door, greatly reduces the pollution risk, is convenient to carry and store due to a cylindrical compact structure, and is convenient to use. And the cell culture equipment is small, portable and simple and convenient to operate.