The invention provides a rapid
screening method for adding prohibited
antibiotics in
cosmetics, which comprises the following steps: carrying out ultrasonic extraction,
centrifugation,
cyclohexane back extraction, sample introduction and C18
chromatographic column separation on a
test sample by using
methanol, extracting parent ions (EIC peaks) of each compound in a
database, and carrying out preliminary qualitative judgment according to the accurate
mass number of the detected substance peaks. The suspected detected substance needs to be independently prepared with reference substance solutions and injected, and confirmation is completed by comparing
ultraviolet absorption spectrum and secondary
mass spectrum characteristics of the detected substance and the reference substance (when a plurality of matching results with the same
mass number appear in qualitative results, the reference substance solutions need to be respectively prepared, and a chromatographic
gradient elution procedure is properly adjusted according to needs; and baseline separation is achieved. The content of the detected substance is obtained by performing
standard curve fitting calculation on the primary
mass spectrum signal. According to the procedure provided by the invention, under the condition that 90% of reference substances are deficient, rapid screening of ten types and 296
antibiotics in
cosmetics can be completed through one experiment.