Diagnostic kit and application of coiled-coil domain containing protein 49 (CCDC49) in preparing gastric cancer early-stage diagnostic reagent

A technology of CCDC49 and diagnostic kits, applied in the field of biological sciences, can solve the problems of high sensitivity and high specificity diagnosis of gastric cancer, and achieve the effect of easy operation and high specificity

Active Publication Date: 2012-09-19
SHANGHAI JIAO TONG UNIV
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  • Abstract
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Problems solved by technology

[0007] The first purpose of the present invention is to provide a diagnostic kit to solve the technical problem that the gastric cancer diagnostic kits in the prior art cannot realize early diagnosis of gastric cancer with high sensitivity and high specificity
[0008] The second object of the present i...

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  • Diagnostic kit and application of coiled-coil domain containing protein 49 (CCDC49) in preparing gastric cancer early-stage diagnostic reagent
  • Diagnostic kit and application of coiled-coil domain containing protein 49 (CCDC49) in preparing gastric cancer early-stage diagnostic reagent
  • Diagnostic kit and application of coiled-coil domain containing protein 49 (CCDC49) in preparing gastric cancer early-stage diagnostic reagent

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Embodiment Construction

[0037] The present invention will be further described below in conjunction with the embodiments and the accompanying drawings.

[0038] 1. Expression, purification and identification of CCDC49:

[0039] The protein CCDC49 is obtained by overexpression induced by genetically engineered Saccharomyces cerevisiae using galactose, and then separated and purified by agarose affinity medium (glutathione). The results of silver staining and Western-Blotting identification Respectively as figure 1 and figure 2 shown.

[0040] 2. Preparation of serum samples:

[0041] Centrifuge the whole blood sample at 1000g for about 20 minutes after standing at room temperature for 2 hours or overnight at 4°C, and take the supernatant for immediate detection; or aliquot and store the sample at -20°C or -80°C, but avoid Freeze and thaw repeatedly. Thawed samples should be centrifuged again prior to testing. The tested samples cannot contain NaN 3 , because NaN 3 Inhibits horseradish peroxi...

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Abstract

The invention relates to the field of the biological science, in particular to a diagnostic kit and an application of coiled-coil domain containing protein 49 (CCDC49) in preparing a gastric cancer early-stage diagnostic reagent. The diagnostic kit is used for diagnosing the early-stage gastric cancer. The diagnostic kit comprises an elisa plate, human protein CCDC49, standard serum, an enzyme labeled reagent, enzyme substrate solution, confining liquid, sample diluent, detergent liquid and termination liquid, wherein the human protein CCDC49 is wrapped on the elisa plate. Compared with the prior art, the diagnostic kit has advantages that 1, a commodity diagnostic kit which is sensitive, safe, reliable and easy to operate is provided, the immunoglobulin m (IgM) antibody for resisting the protein CCDC49 in the human serum can be qualitatively determined, and the early diagnosis of the gastric cancer can be assisted; and 2, the specificity of the provided serum biological labeled protein CCDC49 is 81 percent, the sensitivity is 82 percent, and the characteristics of high specificity and high sensitivity can be realized.

Description

technical field [0001] The invention relates to the field of biological sciences, in particular to a diagnostic kit and the application of CCDC49 in the preparation of early diagnostic reagents for gastric cancer. Background technique [0002] Gastric cancer is a common malignant tumor that endangers human health. According to the statistics of the World Health Organization (WHO) in 2008 (World Health Organization Annual Statistics, 2008), the death rate of gastric cancer patients ranks second in the world. In 2006 WHO statistics showed nearly 950,000 new cases and about 700,000 deaths from this disease. In my country, there are more than 300,000 new gastric cancer patients each year, and about 160,000 people die from gastric cancer. The mortality rate ranks first among malignant tumors, which seriously threatens people's lives and health (Chen Zhu, the third national retrospective sampling survey report on causes of death, 2008) . In order to reduce the mortality rate and ...

Claims

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Application Information

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IPC IPC(8): G01N33/68
Inventor 杨丽娜陶生策刘炳亚王靖方郭书娟朱正纲李建芳燕敏
Owner SHANGHAI JIAO TONG UNIV
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