Diagnostic kit and application of coiled-coil domain containing protein 49 (CCDC49) in preparing gastric cancer early-stage diagnostic reagent
A technology of CCDC49 and diagnostic kits, applied in the field of biological sciences, can solve the problems of high sensitivity and high specificity diagnosis of gastric cancer, and achieve the effect of easy operation and high specificity
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[0037] The present invention will be further described below in conjunction with the embodiments and the accompanying drawings.
[0038] 1. Expression, purification and identification of CCDC49:
[0039] The protein CCDC49 is obtained by overexpression induced by genetically engineered Saccharomyces cerevisiae using galactose, and then separated and purified by agarose affinity medium (glutathione). The results of silver staining and Western-Blotting identification Respectively as figure 1 and figure 2 shown.
[0040] 2. Preparation of serum samples:
[0041] Centrifuge the whole blood sample at 1000g for about 20 minutes after standing at room temperature for 2 hours or overnight at 4°C, and take the supernatant for immediate detection; or aliquot and store the sample at -20°C or -80°C, but avoid Freeze and thaw repeatedly. Thawed samples should be centrifuged again prior to testing. The tested samples cannot contain NaN 3 , because NaN 3 Inhibits horseradish peroxi...
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