103 results about "Fluorescence spectrometry" patented technology

Provided are a fault diagnosis apparatus and method based on fluorescence multivariate correction analysis of transformer oil, relating to the field of transformer fault diagnosis technology. Thus, the problem of large volume and weight of the apparatus, high costs, and inconvenience to use caused when in the related art, a fluorescence spectrometer is directly used to acquire the fluorescence spectrum of transformer oil is solved. The monochromatic excitation light of an optimal excitation wavelength generated by a fluorescence excitation source is used to excite the transformer oil in a fluorescence excitation detection apparatus to generate fluorescence. The fluorescence excitation detection apparatus generates the fluorescence according to the input monochromatic excitation light and inputs the fluorescence to a fluorescence signal acquisition and analysis apparatus. Since the fluorescence signal acquisition and analysis apparatus acquires and analyzes the fluorescence signal emitted by the transformer oil by using a multivariate correction filter group, the emission monochromator component of the fluorescence spectrometer is replaced. In this manner, the equipment costs and the equipment volume are reduced, and data processing is rapid. Thus, the cost performance of the fault detection of the transformer oil is improved, thereby implementing the engineering application of the fluorescence monitoring technology in the online diagnosis of the transformer failure.

The invention provides a method for rapidly detecting wastewater pollutants by atomic fluorescence spectrometry, and belongs to the technical field of wastewater treatment.The method comprises the steps that atomic fluorescence spectrum data of a wastewater sample are collected to establish a spectrum interference matrix to characterize the spectral line overlapping condition between elements; abnormal values are recognized through statistical analysis, a spectrum outlier matrix is built, abnormal fluorescence intensity distribution is recorded, sample matrix non-characteristic fluorescence signals are measured, a background fluorescence interference matrix is built, background interference intensity is quantized, system signal change rules are continuously monitored and detected, and a signal drift tendency matrix is built to predict the signal drift tendency. A spectrum interference matrix and a background fluorescence interference matrix are processed through a spectrum analytic function, compensation factors are calculated, spectrum overlapping and background interference influences are corrected, system drift is corrected in real time, and a final wastewater pollutant concentration detection result is output, so that the technical problem of unstable detection precision caused by coupling of multiple interference factors is solved.

The invention relates to the technical field of water quality detection and evaluation, and provides an evaluation method of a sewage treatment process, which can trace the degradation conditions of different sewage treatment processes on different molecular weight components of organic pollutants by combining an ultrafiltration technology and a three-dimensional fluorescence spectrometry, and can quickly evaluate refractory soluble organic matters in sewage at the same time. In the evaluation process, the separation operation of organic matters is simplified through ultrafiltration, and the cost is reduced; the degradation conditions of different molecular weight components of organic pollutants by different sewage treatment processes can be traced by combining specific ultrafiltration conditions with three-dimensional fluorescence spectrum analysis and a specific calculation formula for the content of the refractory soluble organic matters specified by the invention, and the refractory soluble organic matters in the sewage can be rapidly evaluated.

The invention discloses a method for detecting soil active organic carbon based on fluorescence spectrometry, and belongs to the technical field of soil detection. The method comprises the following steps: sequentially drying, sieving and grinding fresh soil to obtain a soil sample, and sequentially performing active organic carbon extraction, purification, decoloration and pH regulation on the soil sample to obtain a soil liquid to be detected. The method comprises the following steps: adding fluorescein into a soil liquid to be detected to form a soil internal standard detection liquid, obtaining a soil fluorescence spectrum through three-dimensional fluorescence spectrum scanning, carrying out background deduction and correction coefficient correction, and calculating the active organic carbon concentration of the soil by using tryptophan, tyrosine and SMP fluorescence concentration standard curves. According to the method, an internal standard method is combined with a fluorescence spectrum technology, and through multi-step sample pretreatment and spectrum correction, the detection accuracy and the anti-interference capability are improved, the active organic carbon concentration of the soil can be rapidly and accurately determined, and an effective means is provided for soil carbon cycle research and quality evaluation.

The invention discloses a multi-element information acquisition and analysis system based on telemetering drifting buoys, and relates to the technical field of marine environment monitoring. A three-dimensional fluorescence spectrometer, a temperature-salinity-depth sensor, a positioning module and a miniature water sampler are carried on each telemetering drifting buoy; the system is used for collecting algae fluorescence spectrums, water quality parameters and position data in real time, inputting the data into a trained random forest model to output the red tide occurrence probability, triggering multi-level early warning in combination with a preset expert rule engine, linking air and shore-based equipment and a data storage system, and utilizing laboratory true value data obtained by a miniature water sampler to determine the red tide occurrence probability. And performing incremental training on the random forest model every day, thereby dynamically optimizing internal structure parameters of the model. The method effectively improves the prediction probability of the red tide.

This invention proposes an online quality detection method for An Shen Bu Nao Liquid based on multispectral fusion. Addressing the bottlenecks of traditional detection techniques, such as long offline processing time, weak multi-index detection capabilities, and poor dynamic adaptability, this method achieves intelligent quality control throughout the entire production process. The device integrates ultraviolet-visible spectroscopy, near-infrared spectroscopy, and fluorescence spectroscopy modules. It constructs a multi-scale residual convolutional network to extract ultraviolet-visible spectral features, uses a two-way LSTM to analyze the fluorescence excitation-emission matrix, and employs dynamic baseline correction of the ultraviolet spectrum. This improves the detection accuracy of icariin content by 40% compared to traditional HPLC methods, and achieves a heavy metal residue detection limit of 0.05 ppm, providing an innovative solution for the intelligent production of traditional Chinese medicine preparations.

The invention belongs to the technical field of X-ray fluorescence spectrum analysis, and provides a WDXRF testing method based on polyester film pretreatment and application thereof.The method comprises the steps that a sample to be tested is placed in a sample cup, covered with a polyester film and pressed into a tablet through a hydraulic machine, the tablet is placed in a mold, and a WDXRF test is conducted; the parameters of the hydraulic machine comprise that the target pressure is 300-400 kn, and the pressure maintaining time is 5-20 s; the pressing time is 3-5 s; the sampling time is 5-7 s; the sample adding depth is 7-9 s. The method disclosed by the invention is simple, convenient and feasible, is low in cost, can be widely applied to WDXRF tests of various special samples, and has extremely high practical value. According to the method, the polyester film is used for treating the special sample, so that the test of the special sample which cannot be tested originally is realized, the change of sample components is avoided, the side effect is small, the accuracy is high, and the test method has an important application prospect.

The invention provides a method for establishing a metallurgical lime burning reduction model and a method for establishing a calcium-magnesium-silicon content model in metallurgical lime, and further provides application of the method for establishing the models. The establishment method of the model provided by the invention comprises the following steps: preparing a series of standard samples of which the ignition loss and the content of each component have a proper gradient range, directly melting and flaking, weighing the mass of the molten flaking, and carrying out fitting regression on the loss amount of the sample before and after melting and a standard ignition loss value, so as to establish an ignition loss correction model; after dilution ratio correction is carried out on the measured value of each component, iterative regression calculation is carried out on the measured value and the burning loss, and a basic effect correction model caused by the burning loss is established; therefore, a model for rapidly determining the burning loss of the metallurgical lime and the contents of chemical components such as calcium oxide, magnesium oxide and silicon dioxide by using the fuse piece weighing correction-X-ray fluorescence spectrometry is established, and accurate determination of the burning loss of the metallurgical lime and the contents of the calcium oxide, the magnesium oxide and the silicon dioxide can be realized.

The invention belongs to the technical field of environmental monitoring, and particularly relates to a water pollution source identification method based on adaptive three-dimensional fluorescence spectrum (3D-EEM) analysis and a multi-scale convolutional neural network (ACNN-MS). Specifically, the invention provides a method combining a self-adaptive three-dimensional fluorescence spectrum analysis technology and a multi-scale convolutional neural network (MS-CNN), 3D-EEM data are obtained through an improved high-sensitivity three-dimensional fluorescence spectrometer, residual connection is introduced in model construction to avoid the gradient disappearance problem, and the method has the advantages that the method is simple and easy to implement, and the real-time performance of the 3D-EEM data is improved. And a new adaptive wavelength selection algorithm (AWSSA) is utilized to screen the most representative wavelength interval for high-precision calculation, so that the defects of high detection limit, low precision, need of opening a black box and difficulty in traceability positioning of a traditional spectrum and algorithm are overcome. According to the method, the pollution source can be efficiently positioned, the early warning information can be provided, and the quick response capability of the water body pollution event is remarkably improved.

This invention relates to a method, system, medium, and product for X-ray fluorescence spectrometry detection of bauxite, belonging to the field of bauxite detection technology. The method first acquires fluorescence spectral data and mining area environmental fingerprint data of the sample to be tested, and determines the initial concentration by background noise stripping. Then, a mineral phase interference network is constructed, and the interfacial radiation attenuation rate and secondary fluorescence excitation probability are calculated based on mineral phase characteristics to generate an interphase coupling interference matrix. Next, a stress field model is constructed based on environmental parameters, and the interference matrix is ​​corrected using the lattice distortion coefficient. The coexisting mineral phase combination is input into the environmental adaptive interference model to determine the nonlinear matrix effect deviation value. Finally, iterative compensation correction is performed until the difference between adjacent correction values ​​is less than a threshold, outputting accurate grade data of the target metal element. Implementing the technical solution provided in this application can improve the accuracy of X-ray fluorescence spectrometry detection of bauxite.

The invention relates to a method for the X-ray fluorescence analysis of an object to be analysed, which method comprises: - focusing an X-ray measurement beam (10) on or in the object to be analysed, the X-rays being suitable for exciting an emission of X-rays by the object to be analysed; - two-dimensionally scanning the measurement beam (10) over the object to be analysed at a plurality of measurement points on the object to be analysed; - two-dimensionally detecting, using X-ray fluorescence spectrometry means (3), the X-rays emitted by the object along a two-dimensional detection axis (13), at the plurality of measurement points; - identifying at least one measurement point of interest from among the plurality of measurement points; and - confocally detecting, using X-ray fluorescence spectrometry means (4), the X-rays emitted by the object at the at least one measurement point of interest in a measurement volume and along a confocal detection axis (12), the focusing means (5, 6) being configured to generate the measurement volume. The invention further relates to a portable device used by the method, the measurement volume being offset outwards relative to a periphery of the device.

The invention relates to a synchronous high-sensitivity online detection method and system for gaseous mercury and granular mercury, which adopts the coupling and quality control technology of an X-ray fluorescence spectrometry detection system and an atomic absorption spectrometry detection system, and performs system integration of equipment. The device mainly comprises a gas sampling, transmission and pretreatment part, a filter membrane acquisition module and a high-selectivity enrichment module, a dual-detection system mercury analysis unit, a data acquisition, processing and transmission part and a man-machine interaction part, and a novel dual-detection method capable of realizing synchronous on-line high-precision measurement of Hg0, Hg < 2 + > and Hg polymorphic mercury is established. Compared with the prior art, high-precision and high-accuracy measurement of gaseous mercury is realized.

One aspect of the invention relates to a method for determining at least one microstructural property of a metal alloy on the surface of a part manufactured in said metal alloy.

The invention relates to the technical field of analysis and detection, in particular to a sensibilization method for analyzing heavy mass number elements by X-ray fluorescence spectrometry, which comprises the following steps: placing a sample to be detected in a grinding tank, adding ultrapure water and a grinding medium, and crushing by a circulating ball milling process until a superfine powder sample in a target particle size range is obtained; a large amount of liquid in a sample is rapidly removed by adopting a suction filtration method, the sample is dried in a sterile dust-free drying oven, the dried sample is ground again, and the method is suitable for analysis and detection of Ga, Br, Bi, Pb, Th, U, Ca, Y, Zr and Mo elements. According to the invention, a small amount of sample is processed by using a rapid and simple ultrafine crushing technology, the sample is rapidly crushed to a target particle size range, the problem of low heavy mass number element analysis sensitivity under the small amount of sample is overcome, the signal detection intensity is improved, and the heavy mass number element analysis sensitivity of the X-ray fluorescence spectrometry is effectively improved.

The invention discloses a method for determining and analyzing lead, cadmium, iron and zinc in lead smoke dust through X-ray fluorescence spectrometry, and belongs to the technical field of determination and analysis of components of comprehensively recycled lead smoke dust in zinc smelting production. The method comprises the following steps: preparing an analysis sample by adopting a direct tabletting method without acid digestion of a sample and directly preparing soot powder under the working pressure of 30-40T and the dwell time of 15 seconds. A multiple regression correction method is adopted to correct matrix interference, a Pb L beta 1 element spectral line is selected to eliminate overlapping interference of a Pb K alpha 1.2 spectral line and an As K alpha 1.2 spectral line, and then a correction formula is added. The sample does not need to be subjected to complex chemical treatment, the original state of the cigarette ash sample can be kept, and multiple analysis or other subsequent tests can be carried out on the same batch of samples. The method can be used for simultaneously analyzing multiple elements, namely simultaneously determining multiple elements in the soot from macroelements to microelements such as Pb, Zn, Cd, Fe and the like, so that the analysis efficiency is greatly improved.

The application discloses a chemical analysis method for detecting oxides in carbon-containing silicon carbide refractory materials by a non-chemical reagent oxidation method. First, raw materials are crushed, dried and cooled to obtain a treated sample; the silicon carbide content in the treated sample is detected; the treated sample is subjected to a calcination oxidation reaction, and is cooled after the reaction; the weight loss after calcination is calculated; the sample with a tested silicon carbide content of zero is weighed, mixed flux is added, and the sample is fused into a glass sheet; the glass sheet is analyzed and detected to obtain the actual content of the target oxide; finally, data correction is performed to obtain the content of each oxide in the raw materials. The method effectively solves the problems of the use of hazardous chemicals in traditional wet analysis and the environmental and safety problems caused by waste gas and waste liquid treatment, and solves the problem that the refractory materials containing silicon carbide cannot be detected in GB / T21114-2019 "Refractory Materials X-ray Fluorescence Spectrometric Chemical Analysis Fused Glass Sheet Method", so that the detection method is more green and environmentally friendly.

The present invention provides a reagent for detecting the DNA barcode of Asarum heterotropoides Fr. Schmidt var. mandshuricum (Maxim.) Kitag. and a fluorescence detection method for Asarum heterotropoides Fr. Schmidt var. mandshuricum (Maxim.) Kitag. The reagent of the present invention includes a specifically upstream primer of Asarum heterotropoides Fr. Schmidt var. mandshuricum (Maxim.) Kitag. modified with AuNPs and a specifically downstream primer of Asarum heterotropoides Fr. Schmidt var. mandshuricum (Maxim.) Kitag. modified with a fluorescent label. The sequences of the specifically upstream primer of Asarum heterotropoides Fr. Schmidt var. mandshuricum (Maxim.) Kitag. and the specifically downstream primer of Asarum heterotropoides Fr. Schmidt var. mandshuricum (Maxim.) Kitag. modified with a fluorescent label are respectively: 5’-SH-(CH2)6-ATTGACCACGCGAACTTGTGA-3’; 5’-fluorescent label-ACCGTCAACAAACCTGATTGCT-3’. Detection method: Mix the specifically upstream primer of Asarum heterotropoides Fr. Schmidt var. mandshuricum (Maxim.) Kitag. modified with AuNPs and the specifically downstream primer of Asarum heterotropoides Fr. Schmidt var. mandshuricum (Maxim.) Kitag. modified with a fluorescent label with a DNA template solution of the to-be-detected Asarum species for PCR reaction amplification, and detect the fluorescence spectral value of the obtained product to achieve qualitative or quantitative detection of Asarum heterotropoides Fr. Schmidt var. mandshuricum (Maxim.) Kitag.

The invention provides a fluorescent substance characteristic wavelength quantitative analysis method based on concentration-dependent red shift induced by an inner filter effect. The method comprises the following steps: acquiring fluorescence spectrums of a series of fluorescent substance solutions with a series of concentrations; preprocessing the spectrum, and extracting characteristic wavelengths (peak wavelength lambdamax and wavelengths lambdal and lambdar corresponding to specific intensity nImax at two sides of a peak value); constructing a linear model of the characteristic wavelength and the concentration, and determining an optimal fitting model by dynamically optimizing a scale factor n and a wavelength combination strategy; and high-precision and wide-range quantitative analysis of the concentration of the solution to be detected is realized by using the optimal model. According to the method, the linear detection range can be expanded to more than 5 times that of a traditional method, the relative average error is smaller than 1%, the method is not influenced by hardware parameter fluctuation, and the range and precision of fluorescence quantitative analysis are remarkably improved.

The invention belongs to the technical field of agriculture, and discloses a herbicide efficacy dynamic evaluation method and system, efficacy evaluation is carried out through two methods of a colorimetric method and a plant fluorescence spectrometry, weights of two evaluation results are dynamically adjusted according to environmental parameters, the two evaluation results are synthesized according to the weights to obtain a final efficacy evaluation result, and the efficacy evaluation result is evaluated according to the weight. The environment interference can be effectively overcome, and the accuracy and reliability of herbicide efficacy evaluation are improved.

A particle analysis system comprising: a light detector that acquires light generated by irradiating a particle with excitation light; and an information processing unit that outputs a spectral plot including spectrum information of an autofluorescence population specified in a two-dimensional plot of measurement data each of which corresponds to the acquired light and spectrum information of the measurement data and that records the spectrum information of the autofluorescence population as an autofluorescence reference spectrum in a fluorescence separation process.

The invention belongs to the technical field of chemical analysis, and particularly relates to an X-ray fluorescence spectrometry method for rapidly and synchronously determining primary and secondary components of silicon, calcium, aluminum and phosphorus in a silicon nitride alloy. According to the method, a crucible is lined with lithium tetraborate, an oxidizing agent and a fusing agent in a certain proportion are added, and a proper sample pre-melting oxidation procedure is set, so that the rare earth nitrogen alloy can be made into a glass casting sheet sample meeting the measurement requirement of an X-ray fluorescence instrument, and the content of various components in the alloy can be synchronously tested through the X-ray fluorescence spectrometry; the detection time is greatly saved, the electricity and gas expenses are saved, the detection cost is reduced, and meanwhile, the blank of no method for rapidly and accurately determining the elements in the rare earth nitrogen alloy at the same time is filled up.

The present invention provides a planar XY micro-motion device for a coating thickness gauge, comprising: a sample carrier, a slide seat, a slide middle seat and a slide base, wherein the slide middle seat is slidably connected to the slide base in the X direction, and the slide carrier is slidably connected to the slide middle seat in the Y direction; an X-axis transmission positioning seat is provided on one side of the slide base, and an X-axis screw nut seat is provided on one side of the slide middle seat. The sample carrier is mounted on the slide seat, which enables an X-ray fluorescence spectrometry coating detector to accurately locate the position of a small sample to be inspected, thereby improving detection efficiency. The planar XY micro-motion mechanical device in the present invention can be conveniently installed on a coating thickness gauge, and the position of the item to be inspected can be adjusted after closing the instrument protective cover, thereby ensuring the controllability of the position of the item to be inspected while protecting operational safety.

The invention provides a method for quantitatively identifying contribution of multi-source phosphorus pollution, relates to the field of water environment management, and aims to accurately analyze sources of multi-source phosphorus pollution of river and lake water bodies. The method comprises the following steps: firstly, determining the pollution source type of a research area, collecting river and lake water and pollution source water samples, synchronously retaining subsamples, determining the phosphorus form concentration, and establishing an associated ledger; then measuring a three-dimensional fluorescence EEM spectrum of a water sample, preprocessing the three-dimensional fluorescence EEM spectrum, extracting a fluorescence component by using a PARAFAC model, calculating a Fmax value, constructing fluorescence component-Fmax-phosphorus concentration associated data, optimizing a projection direction through an immune genetic algorithm, constructing a projection pursuit regression model, and supplementing phosphorus pollution associated characteristic values to form two-dimensional correction data; and finally, inputting correction data by using a MixSIAR model, operating MCMC to simulate and estimate a pollution source contribution proportion, verifying model convergence, and analyzing and visualizing sample point pollution contribution and spatial distribution characteristics. According to the method, the phosphorus pollution traceability accuracy and reliability are improved, a scientific basis is provided for water environment treatment, and drainage basin management and ecological civilization construction are assisted.

The invention relates to the technical field of combination of a testing machine and a fluorescence spectrometer, in particular to an in-situ mechanoluminescence combination interface for combination of the testing machine and the fluorescence spectrometer, and discloses an in-situ mechanoluminescence combination interface for combination of the testing machine and the fluorescence spectrometer, which is used for adjusting the distance, the angle and the relative height between the spectrum combination interface and a detected object by driving a fixed frame to move when a sliding block moves. The requirement for multi-angle accurate adjustment of the spectrum coupling interface is met, and manual pushing by workers is not needed. And the design of combined use of the matching groove and the fixing rod avoids angle and distance changes caused by mistaken touch in the use process. The spectrum coupling interface provided by the invention can be used for coupling an FLS980 steady-state transient fluorescence spectrometer and a tensile testing machine, the exciting light of the spectrometer is guided out by the optical fiber and excites a sample stretched on the testing machine through the spectrum coupling interface, and an emission signal can be guided into the spectrometer through the optical fiber in the stretching process and is detected by a spectrometer detector; therefore, optical parameters such as fluorescence spectrum and service life are obtained.

The application provides a method for identifying bases, drops a DNA solution on a molybdenum disulfide film to prepare a DNA testing device, uses a fluorescence spectrometer to detect fluorescence spectrum data of the molybdenum disulfide under the influence of bases, carries out characteristic matching degree comparison analysis based on the fluorescence spectrum curve of the molybdenum disulfide under the influence of unknown bases and the fluorescence spectrum curve of the molybdenum disulfide under the influence of known bases, and determines which kind of known base the unknown base is according to the matching degree comparison analysis result; the application can effectively identify bases by using the DNA and MoS2 adsorption and charge transfer mechanism, and has a lower requirement for the concentration of the used DNA solution to be tested; meanwhile, the MoS2 has the advantages of low cost and simple preparation, and compared with the prior art, the application has the advantages of low-concentration trace DNA base effective identification, simple device structure, simple operation and low cost.

This invention provides a fluorescent probe for label-free, multifunctional, low-cost, and high-throughput real-time monitoring of tyrosine phosphorylation. Our designed fluorescent molecule specifically binds to copper ions in aqueous solution, leading to fluorescence quenching. Adding a phosphorylated substrate peptide with tyrosine residues to the quenched system elicits a dramatic change in fluorescence, with the solution color changing from purple to red. However, no significant fluorescence change is observed upon adding non-phosphorylated substrate peptides or other phosphate-related ions. This on-off fluorescence response enables real-time monitoring of tyrosine phosphorylation, with a detection limit of 100 nM. This method can also be applied to the rapid screening of PKA kinase inhibitors and to the detection of serine, threonine, and phosphorylated peptides at mono- and diphosphorylated sites. The greatest advantage of this fluorescence spectroscopy method is that it does not require antibodies or radioactive isotopes, has simple operating procedures, and is highly suitable for the real-time detection of protein tyrosine phosphatase activity.

The invention discloses a method for detecting the total silicon content in a sample, which comprises the following steps of: mixing the sample and a flux, putting the mixture into a lining crucible for pre-oxidizing and melting, thoroughly decomposing refractory matters in the sample and eliminating the reducibility of the refractory matters, melting the pre-oxidized sample to prepare a glass sheet, and measuring the total silicon content in the sample by using an X-ray fluorescence spectrometry. The method is short in time consumption, small in pollution, high in precision, accurate and reliable, can meet the requirements for accurate analysis and quality control of the total silicon content in the steelmaking reducing agent, and ensures that materials are efficiently and stably applied.

The invention belongs to the technical field of material component analysis, and particularly relates to a method for quantitatively detecting components of Al2O3.TiO2 spraying powder through an X-ray fluorescence spectrum. The method comprises the following steps: mixing powder standard substances of components SiO2, TiO2, Fe2O3 and Al2O3 to prepare standard mixed samples with different gradient contents, preparing a glass fuse piece, detecting by adopting an X-ray fluorescence spectrometry, and establishing a standard curve of the contents of the components and the fluorescence intensities by respectively taking the mass percentage contents of SiO2, TiO2 and Fe2O3 as horizontal coordinates and the fluorescence intensities corresponding to Si, Ti and Fe elements as vertical coordinates. And detecting the glass fuse piece of a to-be-detected actual sample, and respectively substituting the fluorescence intensity of the element into the standard curve corresponding to each component to obtain the content of each component in the actual sample. According to the method, the operation steps are simplified, the detection efficiency is improved, and a new analysis means is provided for component detection of the spraying powder.

The application provides a monovalent mercury detection method, and relates to the field of analytical detection technology. The monovalent mercury in a to-be-detected sample is extracted by 2-mercaptoethanol aqueous solution, and the obtained to-be-detected sample liquid is subjected to high performance liquid chromatography-atomic fluorescence spectrometry detection. In the high performance liquid chromatography separation process, the monovalent mercury is completely disproportionated into zero-valent mercury and divalent mercury, and is separated by high performance liquid chromatography, and is subjected to atomic fluorescence spectrometry detection respectively. The mercury of all valence states is reduced by a reducing agent and forms mercury vapor, and then generates a fluorescence signal, and sequentially peaks with a retention time, so that the zero-valent mercury detection peak area and the divalent mercury detection peak area are obtained. According to the zero-valent mercury detection peak area and the divalent mercury detection peak area, the detection concentration of divalent mercury and the detection concentration of zero-valent mercury are obtained by using an external standard method. The concentration of monovalent mercury in the to-be-detected sample liquid is calculated, the accuracy and the sensitivity of detection are high, and efficient analysis and detection of monovalent mercury are realized.