Time resolution fluorescence spectral measuring and image forming method and its device

Abstract

A method of using picosecond scan camera to simultaneously obtain fluorescent light spectrum and fluorescent lifetime of sample includes focusing blue violet light emitted by laser on sample by objective to excite sample single photon, collecting fluorescent from sample then splitting it and focusing it to be image on photoelectric cathode of picosecond scan camera, setting dispersion direction of fluorescent to be the same as slit direction of said camera but to be vertical to scan direction in order to measure out fluorescent lifetime of different light spectrum simultaneously under coaction of scan circuit and image intensifier deflection system.

Description

technical field [0001] The invention is a measurement method and device capable of simultaneously acquiring fluorescence spectrum and fluorescence lifetime information of biological samples. Since the fluorescence lifetime and the fluorescence spectrum are sensitive to the microenvironment of the fluorescent molecule and the type of the fluorophore molecule, the simultaneous measurement of the two can provide complementary functional information for biomedical detection and analysis. The present invention will be used in biology, It has important applications in research fields such as medicine and material science, as well as clinical medical diagnosis. Background technique [0002] Fluorescence spectroscopy is a powerful tool widely used in biomedical research and clinical diagnosis. Since the fluorescence spectrum emitted by fluorescent molecules depends on the components of the fluorophore, the molecular species and its energy level structure, the measurement of the flu...

AI Technical Summary

Problems solved by technology

The current commonly used time-resolved fluorescence spectroscopy devices cannot meet these requirements. The reason is that the detectors used for time and spectrum measurement are separate devices, which do not combine spectrum measurement and lifetime measurement well. Therefore, the obtained ones are either some Limited fluorescence lifetime of discrete spectra (such as combining a time-correlated single photon counter with a polychromator), or requires multiple measurements to obtain time-resolved spectral information of the sample, and the measurement time is very long (such as scanning monochromator and pulsed Sampling measurement or combination of imaging spectrometer and gated pulse image intensifier)

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