The invention relates to the technical field of
fluorescence detection, in particular to a three-dimensional
fluorescence detection
correction method based on double light sources, and the
correction method comprises the following steps: obtaining double
light source optical parameters, sample
cell geometric parameters and original
fluorescence intensity data, the geometric parameters of the
sample pool comprise the length, width, height,
pool wall
reflection coefficient and sample
attenuation coefficient of the
sample pool; and constructing a
light intensity distribution model in a three-dimensional space of the sample
cell based on the optical parameters of the double light sources and the geometric parameters of the sample
cell. According to the invention, accurate characterization of the
light intensity of any spatial point in the sample cell is realized,
light intensity deviation caused by various factors is quantified from the source, personalized correction is carried out on each spatial point, the influence caused by
optical path difference at different positions,
light source fluctuation and element error is effectively offset, and the accuracy and accuracy of the detection result are improved. The consistency of detection values of standard substances with the same concentration in different areas of the
sample pool is greatly improved, and the
linearity of a
standard curve is remarkably optimized.