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371 results about "Fluorescence response" patented technology

Integrated circuit packaging quality evaluation method and system based on nondestructive testing technology

The invention provides an integrated circuit packaging quality evaluation method and system based on a nondestructive testing technology, and relates to the technical field of integrated circuits, and the method comprises the steps: obtaining a three-dimensional fluorescence response signal of a packaging structure through a two-photon excitation microscopic system, and building a stress distribution characteristic graph and a stress gradient grading graph through the analysis of the spatial distribution of the fluorescence signal; a quantum state nitrogen vacancy probe array is arranged in the stress abnormal area, electromagnetic field response signals of the quantum state nitrogen vacancy probe array under the stress effect are collected, and accurate mapping of a stress field and an electromagnetic field of the packaging structure is achieved; based on the obtained multi-physical field data, establishing a thermal electromagnetic coupling equation set, and calculating an interaction relationship among the physical fields; by analyzing evolution characteristics of each detection area under the multi-field coupling effect, a stability evaluation system including a mechanical failure risk and an electromagnetic failure risk is established, and accurate evaluation of the quality grade of the packaging structure is realized.
Owner:BEIJING YANHUANG GUOXIN TECH CO LTD

Cd (II)-based metal organic framework crystalline material as well as preparation method and application thereof

The invention relates to the technical field of fluorescence sensing development, in particular to a Cd (II)-based metal organic framework crystalline material as well as a preparation method and application thereof. The chemical formula of the Cd (II)-based metal organic framework crystalline material is {[Cd3 (H2O) 2 (DMA) 2 (5-CMOIA) 2]. 6 / 5H2O} n, the organic framework material is characterized by an X-ray single crystal diffractometer, and the result shows that the organic framework material is crystallized in a triclinic system P-1 space group, the asymmetric unit of the organic framework material is composed of three Cd (II) ions, three Cd (II) ions, three Cd (II) ions, three Cd (II) ions, three Cd (II) ions, three Cd (II) ions, three Cd (II) ions, three Cd (II) ions, three Cd (II) ions, three Cd (II) ions, three Cd (II) ions, three Cd (II) ions, three Cd (II) ions and three The complex is composed of two deprotonated (5-CMOIA) 3-ligands, two coordinated DMA solvent molecules, two coordinated water molecules and six fifths of crystal water molecules. The solid fluorescence spectrum of the Cd (II)-based metal organic framework crystalline material is measured, the fluorescence response of the Cd (II)-based metal organic framework crystalline material to metal ions in a solution is explored, and the result shows that the Cd (II)-based metal organic framework crystalline material can effectively recognize Hg < 2 + > from different ions and can be applied to the field of fluorescence sensing.
Owner:SHAANXI SCI TECH UNIV

Ratio-type molecular imprinting fluorescence sensing detection method based on metal organic framework and nitrogen-doped carbon dots

The invention provides a ratio type molecular imprinting fluorescence sensing detection method based on a metal organic framework and nitrogen-doped carbon dots, the advantages of N-CDs, Eu-MOF and a molecular imprinting layer are fused, in the presence of target molecules, the blue fluorescence (430nm) of the N-CDs is effectively enhanced due to non-radiative recombination caused by the surface defect state of the CDs, and the red fluorescence (660nm) of the Eu-MOF remains unchanged. According to the ratio type molecularly imprinted fluorescent sensing detection method, while a fluorescent response signal is displayed, the color of the imprinted polymer changes along with different concentrations of the target molecules, and sensitive visual detection of the target molecules can be realized, so that specific sensitive detection of the target molecules in a complex sample is realized; the method has the advantages of high sensitivity, simplicity and convenience in operation, good reproducibility, strong anti-interference performance and the like. According to the method, rapid and sensitive detection of organophosphorus pesticides, toxic agents and other imprinted molecules can be realized by preparing imprinted polymers of different target molecules without fluorescence interference, and the method has a wide application prospect.
Owner:RES INST OF CHEM DEFENSE PLA ACAD OF MILITARY SCI

Fingerprint extraction method based on mechanochromic pyrenyl derivative

The invention belongs to the technical field of novel fluorescent materials, and particularly discloses application of a mechanical color change pyrenyl derivative in latent fingerprint fluorescence imaging and extraction. The mechanical color-changing pyrenyl derivative can be selectively combined with fingerprint residual substances under the excitation of ultraviolet light through the unique fluorescence response characteristic, so that potential fingerprints form high-contrast fluorescence imaging on smooth or rough surfaces (such as glass, metal, plastic and the like), and the mechanical color-changing pyrenyl derivative is compatible with conventional camera equipment to realize rapid acquisition. The compound design is based on a modular synthesis route, and has the advantages of simple and convenient synthesis process, controllable raw material cost, high reaction yield and the like. The product has excellent thermal stability and chemical stability, can be stored at normal temperature and does not need special treatment, and the practical application threshold is greatly reduced. Compared with a traditional fingerprint developing technology, the method has remarkable breakthroughs in the aspects of sensitivity, substrate adaptability and operation convenience, and an efficient, economical and environment-friendly novel solution is provided for the fields of criminal investigation, forensic identification and the like.
Owner:SHANDONG UNIV OF TECH

AI-based liquid crystal glass plate flaw detection method and system

The invention relates to the technical field of quality detection, in particular to an AI-based liquid crystal glass plate defect detection method and system, comprising multi-modal data acquisition, adaptive preprocessing, dynamic intelligent detection, defect cognition decision, closed-loop feedback execution, a safety guardrail mechanism and digital twin support. Compared with the prior art which adopts a single imaging mode for surface detection and has the defects that the omission ratio of tiny flaws is high and metal and PR defects are difficult to distinguish, the scheme innovatively introduces a multi-mode collaborative acquisition mechanism, and the detection accuracy is improved through millisecond-level synchronization and space registration of four sets of bright field, dark field, ultraviolet and structured light imaging systems. A comprehensive data cube containing textures, metal features, fluorescence response and three-dimensional morphology is constructed, full-dimensional capture and accurate characterization of micron-sized flaws are achieved, and the detection rate and classification accuracy of complex flaws are remarkably improved.
Owner:BORUI INTELLIGENT MFG TECH (GUANGZHOU) CO LTD

Indole-benzothiadiazole derivative as well as preparation method and application thereof

The invention discloses an indole-benzothiadiazole derivative as well as a preparation method and application thereof. The general structural formula of the indole-benzothiadiazole derivative provided by the invention is shown in the specification, wherein R is selected from one of methyl piperazinyl, diethylamino, azetidinyl and pyrrolidinyl. The derivative can be specifically combined and fluorescently labeled with mitochondrial RNA in cells, and has good selectivity, high fluorescence signal-to-noise ratio and excellent anti-interference capability in a complex biological system. Meanwhile, the indole-benzothiadiazole derivative provided by the invention is simple in synthetic route, easily available in raw materials, good in stability and convenient to store and use, can be specifically combined with RNA and generate strong fluorescence response, is suitable for real-time detection and imaging of in-vitro and in-vivo mitochondrial RNA, and is an efficient mitochondrial RNA micromolecular fluorescent probe.
Owner:GUANGDONG UNIV OF TECH

Full-automatic intelligent multifunctional high-order nonlinear fluorescence dynamics testing method and device

The invention discloses a full-automatic intelligent multifunctional high-order nonlinear fluorescence dynamics testing method and device, and relates to the field of nonlinear optics and nano photonics. An optical testing device composed of an exciting light generation module, an automatic power regulation module, an exciting light modulation module, a sample scanning module, a data acquisition and analysis module and an intelligent control module is established, and under the excitation of incident laser, based on the overall coordination of the intelligent control module, the optical testing device is established. Other modules are driven to complete the dynamic response test of a nonlinear fluorescence sample, and full-automatic intelligent, multifunctional, high-precision, simple, efficient, low-cost and universal fluorescence dynamic test analysis is realized. The problems that traditional manual operation wastes time and labor and the testing precision is low are solved. In addition, the multifunctional integration of the fluorescence response curve, the fluorescence rise time, the fluorescence lifetime and the fluorescence imaging effectively improves the test efficiency and the application scene, and greatly reduces the system cost.
Owner:XIAMEN UNIV

Water pollutant discrimination method and system based on multi-source spectrum fusion

The invention belongs to the technical field of water pollutant identification, and particularly relates to a water pollutant distinguishing method and system based on multi-source spectrum fusion. According to the method, ultraviolet visible spectrum data and three-dimensional fluorescence spectrum data are effectively integrated in a multi-source spectrum data fusion mode, so that the characteristic information of water pollutants is captured, stably appearing characteristic absorption wavebands can be positioned by utilizing the ultraviolet visible spectrum data, and the water pollutants can be accurately detected. The method comprises the following steps: identifying a characteristic sub-region capable of representing the fluorescence response characteristic of a pollutant through three-dimensional fluorescence spectrum data, in a characteristic extraction stage, performing first-order derivative operation on an ultraviolet visible spectrum to position an absorption peak position, calculating curvature distribution of a three-dimensional fluorescence spectrum to identify the characteristic sub-region, and when a fusion characteristic is generated, performing first-order derivative operation on the three-dimensional fluorescence spectrum to identify the characteristic sub-region; according to the method, the key operation parameters are subjected to grid search optimization, and the optimal key operation parameter combination is screened out by performing multiple rounds of iterative training and evaluation on a preset training set and a verification set, so that the recognition performance of the real-time collected water sample is improved.
Owner:CHONGQING UNIV OF TECH

Shrimp pond water quality prediction method based on deep echo state network

The invention discloses a shrimp pond water quality prediction method based on a deep echo state network, and relates to the technical field of shrimp pond water quality prediction, and the method comprises the following steps: deploying an underwater fluorescence sensor in a shrimp pond culture water body to detect a fluorescence response signal of algae, and obtaining algae concentration data in the water body in real time based on the fluorescence signal intensity; dynamic changes of algae in the prawn pond water are monitored in real time; and performing time sequence alignment on the collected algae concentration data, and constructing an algae concentration time sequence data map based on the aligned time sequence data. According to the method, fluorescence sensor data and a short-time feature extraction mechanism are fused, a deep echo state network is combined to model an algae burst trend, and early warning can be realized before red tide outbreak; and the abnormal area monitoring precision is improved by enhancing a sampling mechanism, the problem of slow recognition of emergencies by a traditional model is effectively solved, and the aquaculture water quality risk perception and intelligent management capability is remarkably improved.
Owner:ZHEJIANG DANSHUI FISHERY RESEARCH INSTITUTE (ZHEJIANG DANSHUI FISHERY ENVIRONMENTAL MONITORING STATION)

Visual detection test method for damage crack of solidified soil test piece

The invention discloses a visual detection test method for damage cracks of a solidified soil test piece, and particularly relates to the technical field of geotechnical engineering material detection. The method comprises the following steps: doping fluorescence labeling particles in a solidified soil test piece, and applying periodic load to induce crack formation after curing and forming; synchronously exciting fluorescent particles through an ultraviolet excitation light source, and collecting a short-wave fluorescence response image sequence; inputting the image sequence into a deep residual neural network model, identifying a nonlinear enhancement region of a fluorescence signal, and deducing a crack initiation position and a propagation path; constructing a three-dimensional dynamic model of crack evolution in combination with an image space reconstruction algorithm and time sequence comparison, extracting indexes such as a crack propagation rate, a bending angle and crack density, performing comparative analysis on the indexes and initial parameters of a material, evaluating a microstructure damage development mechanism, and outputting a visual damage evolution result; the method provided by the invention can realize high-precision, non-destructive and dynamic detection of the fine cracks in the solidified soil, and has the advantages of high identification sensitivity, strong modeling precision and good engineering adaptability.
Owner:GUANGDONG UNIV OF TECH

Mycotoxin six-in-one detection card intelligent interpretation system and method based on multi-dimensional data analysis

The invention relates to the technical field of biotoxin detection, and discloses a multidimensional data analysis-based fungaltoxin six-in-one detection card intelligent interpretation system and method.The method comprises the following steps: collecting first toxin detection data of six fungaltoxins, including a fluorescence response time sequence, a colloidal gold response time sequence and an environment variable time sequence; performing feature extraction on the second toxin detection data to obtain second toxin detection data; then establishing a historical detection database, constructing and training a mycotoxin classification model, and obtaining a preliminary recognition result of the six mycotoxins according to the second toxin detection data and the classification model; and finally, carrying out weighted fusion on the preliminary recognition result in combination with an environment variable time sequence to obtain an accurate recognition result. According to the invention, intelligent interpretation of mycotoxin detection is realized, and the detection accuracy and efficiency are greatly improved.
Owner:南京微测生物科技有限公司

Synthesis and Application of Multifunctional Stimuli-Responsive Aggregation-Induced Emission Enhancement Probes

The present invention provides the synthesis and application of a group of multiple stimulus-responsive aggregation-induced emission enhancement probes, belonging to the field of aggregation-induced emission materials. Through a simple three-step method, a D-π-A type molecular skeleton is constructed in the molecule, and a group of pyranone-type aggregation-induced emission enhancement probes are synthesized. This group of probes can undergo non-interfering changes in fluorescence color or intensity under multiple stimuli such as solvents, pressure, humidity, and pH. On the one hand, it makes up for the relatively single variety of AIE template molecules; on the other hand, it breaks through the technical barrier of single response of aggregation-induced emission probes, realizing the possibility that the same molecule can simultaneously respond non-interferingly to multiple external stimuli. The preparation method of this probe is simple, with high yield and sensitive and rapid fluorescence response, and is suitable for various application needs.
Owner:TIANJIN VOCATIONAL INST

Rare earth metal polymer with multiple stimulation responsiveness and preparation method thereof

The invention discloses a rare earth metal polymer with multiple stimulation responsiveness and a preparation method thereof, two monomers SP and VTPY are mixed with methyl methacrylate (MMA) according to a certain proportion, a polymer skeleton Poly (VTPY-co-SP-co-MMA) is prepared through a free radical polymerization reaction, the polymer and Tb (Com) 3.2 H2O are further subjected to a coordination reaction according to a specific proportion, and the rare earth metal polymer with multiple stimulation responsiveness is obtained. The Poly-Tb (Coum) 3 with the stimuli-responsive discoloration characteristic is prepared by the aid of the method. The rare earth metal polymer with methyl methacrylate as a framework and rare earth terbium ions and spiropyran monomers as double emission centers is synthesized, has multiple stimulation response characteristics, provides a brand new strategy and possibility for development of rare earth-based multifunctional intelligent materials, and has wide application prospects. An innovative solution is provided for development of a thermal enhanced rare earth luminescent material, the thermosensitive fluorescence response performance of the material is greatly improved, and a new thought is provided for design of an intelligent temperature response type rare earth luminescent material.
Owner:NORTHWESTERN POLYTECHNICAL UNIV

ANA detection image-text report intelligent generation method and system

The invention discloses an ANA detection image-text report intelligent generation method and system, and relates to the technical field of immunodetection, the system uses different fluorescent markers to mark ANA, signal sensitivity is enhanced, and detection accuracy is ensured. Then, collecting fluorescence signals of different wavelengths by adopting a fluorescence microscope and a multi-wavelength excitation light source, and obtaining dynamic change data of reaction intensity by combining a time-resolved fluorescence imaging technology to generate a multi-channel imaging data set; thirdly, noise removal, signal enhancement and image alignment are carried out through an image preprocessing technology, and data consistency is ensured; based on a multi-channel data set, a convolutional neural network is used for training and testing an initial model, an intelligent detection model is constructed, a fluorescence reaction mode is extracted, finally, a detailed ANA detection report is automatically generated by calculating a comprehensive scoring coefficient and comparing the comprehensive scoring coefficient with a preset threshold value, and an accurate immunodiagnosis basis is provided for doctors.
Owner:GUANGZHOU HUISHAN MEDICAL TECH CO LTD +1

DNA quantitative fluorometer calibration system based on artificial intelligence optimization

The invention relates to the technical field of biomedical analysis instruments, and discloses a DNA quantitative fluorometer calibration system based on artificial intelligence optimization, which comprises a data acquisition module for acquiring a dynamic fluorescence characteristic spectrum for representing the whole process of fluorescence reaction; and an intelligent calibration and diagnosis core model in the data processing unit analyzes the spectrogram by using a long short-term memory network and an attention mechanism so as to generate a multi-dimensional diagnosis result containing predicted concentration, confidence score and interference early warning. According to the method, the dynamic fluorescence characteristic spectrum of the standard substance is used as a scene anchor point, the preset global basic model is finely adjusted, and the special session model is generated, so that the real-time self-adaptive calibration of the change of the instrument and the reagent is realized. According to the method, the defects that a traditional method depends on an end point value, is easily interfered and is static in calibration are overcome, and the accuracy, reliability and long-term stability of a DNA quantitative result are remarkably improved.
Owner:HENAN PROVINCE INST OF METROLOGY

Magnetic aggregation induced emission particle fluorescence-electrochemical nucleic acid dual-detection chip and detection system

The invention discloses a magnetic aggregation induced emission particle fluorescence-electrochemical nucleic acid dual detection chip and system.The chip comprises a chip body and an upper cover, and a plurality of electrochemical reaction detection cavities and fluorescence reaction detection cavities are formed in the left side and the right side of the chip body in the front-back direction respectively; the electrochemical reaction detection cavities and the fluorescence reaction detection cavities are arranged in a one-to-one correspondence manner; each electrochemical reaction detection cavity and each fluorescence reaction detection cavity are provided with a flow channel, and one end of each flow channel is communicated with the electrochemical reaction detection cavity or the fluorescence reaction detection cavity; the other end of the sampling tube is communicated with an MAIE electrochemical detection auxiliary liquid sample adding hole and an MAIE fluorescence detection auxiliary liquid sample adding hole which are formed in the chip main body; a plurality of MAIE electrochemical detection sample adding holes and MAIE fluorescence detection sample adding holes are formed in the upper cover, and corresponding openings are formed in the positions, corresponding to the MAIE electrochemical detection auxiliary liquid adding holes and the MAIE fluorescence detection auxiliary liquid adding holes, of the upper cover.
Owner:ZHEJIANG MEDICAL COLLEGE

Fluorescence detection condition control system and method

The invention relates to the technical field of electrocatalytic oxidation, and provides a fluorescence detection condition control system and method, and the system comprises a strategy library which uses XGBoost to train strategy algorithm models of different water sample water qualities and initial wave bands; the initialization module is used for calling the strategy library to load the water quality type according to a detection environment and setting initial state water sample condition matching; the pre-excitation module is used for lightening the single-band LEDs one by one to obtain an initial fluorescence response value; the self-adaptive detection module is used for calling a strategy algorithm model and generating a wave band combination and excitation intensity configuration according to the initial fluorescence response value; the temperature compensation module monitors the key temperature in real time through a temperature sensor and carries out compensation correction according to a fluorescence error caused by temperature drift; and the analysis report module outputs a Cu < 2 + > concentration detection result. According to the method, the water quality preset strategy library and the temperature compensation mechanism are combined, Cu < 2 + > fluorescence detection under the complex matrix background is achieved, and the accuracy and environmental adaptability of low-concentration heavy metal detection are remarkably improved.
Owner:BAICHENG NORMAL UNIV

Bovine viral diarrhea virus loop-mediated isothermal amplification primer group and application thereof

The invention provides a bovine viral diarrhea virus loop-mediated isothermal amplification primer group and application thereof, and relates to the technical field of biological detection, and the bovine viral diarrhea virus loop-mediated isothermal amplification primer group comprises an outer primer F3, an outer primer B3, an inner primer FIP, an inner primer BIP, a loop primer LF and a loop primer LB. The specificity and the detection comprehensiveness of the kit on bovine viral diarrhea virus gene amplification are ensured; the primer group can realize rapid detection of the bovine viral diarrhea virus loop-mediated isothermal amplification primer group, the detection sensitivity is higher than that of common PCR, and the result is judged through an amplification curve and a Tt value. And judging the result through a product fluorescence reaction according to conditions. The formed kit has the advantages of simplicity in operation, high specificity, no need of special equipment, low cost and easiness in result judgment, and is suitable for on-site rapid detection, clinical diagnosis of grassroots veterinarians or monitoring of epidemic diseases. The technical problem that in the prior art, the LAMP technology is lacked for rapidly and accurately detecting or identifying the bovine viral diarrhea virus is solved.
Owner:BEIJING BIAOCHI ZEHUI BIOTECHNOLOGY CO LTD +1

Preparation method and application of fluorescent ZET-MOGs material

The invention relates to a preparation method and application of a fluorescent ZET-MOGs material, and the method comprises the following steps: placing ZrOCl2. 8H2O in a glass container, fully dissolving ZrOCl2. 8H2O by using deionized water and ultrasonic treatment, and naming as a solution A; 2, 4, 6-tri (4-carboxyl phenyl)-1, 3, 5-triazine (TATB) is placed in deionized water, triethylamine is added and subjected to ultrasonic treatment to be fully dissolved, and a solution B is obtained; mixing the solution A and the solution B according to a volume ratio of 1: 1 to form Zr-MOGs wet gel; the obtained wet gel is washed with deionized water, and Zr-MOGs powder is obtained through freeze drying; eu (NO3) 3.6 H2O and Tb (NO3) 3.6 H2O are dissolved in ethyl alcohol, then Zr-MOGs powder is added, stirring and centrifugal separation are carried out, washing is carried out with ethyl alcohol, and finally light yellow fluorescent ZET-MOGs powder is obtained through vacuum drying. The ZET-MOGs prepared by the preparation method disclosed by the invention has a relatively large specific surface area, relatively high porosity, rich surface functional groups and stable fluorescence response characteristics, and shows efficient removal efficiency and sensitive detection capability on CTC in a water body.
Owner:JILIN UNIVERSITY

Preparation method of water-soluble CsPbBr3 perovskite quantum dot fluorescence sensing array and application of water-soluble CsPbBr3 perovskite quantum dot fluorescence sensing array in rapid detection and inactivation of food-borne pathogenic bacteria

The invention discloses a preparation method of a water-soluble CsPbBr3 perovskite quantum dot fluorescence sensing array and application of the water-soluble CsPbBr3 perovskite quantum dot fluorescence sensing array in rapid detection and inactivation of food-borne pathogenic bacteria. The sensor array is composed of three perovskite quantum dots (respectively named as PQD1, PQD2 and PQD3) with different fluorescence characteristics, wherein the perovskite quantum dots are synthesized at room temperature. The feasibility of constructing the sensing platform is verified through different fluorescence responses after the sensing platform is combined with five food-borne pathogenic bacteria (escherichia coli, staphylococcus aureus, salmonella typhimurium, listeria monocytogenes and pseudomonas aeruginosa). The relative signal intensity change is further analyzed through a machine learning algorithm, and rapid recognition and identification of pathogenic bacteria are achieved. In addition, the sensing array shows high practicability and accuracy in actual sample detection, and pathogenic bacteria can be effectively inactivated after 30 minutes of detection. The invention provides a novel efficient and reliable technical solution for food safety detection.
Owner:HEFEI UNIV OF TECH

Hydrogen peroxide probe as well as preparation method and application thereof

The invention relates to a hydrogen peroxide probe and a preparation method and application thereof, the hydrogen peroxide probe connects pyridinium to a difluoro boron indole electron acceptor, can induce fluorescence quenching by distorting intramolecular charge transfer, generates weak fluorescence when the concentration of hydrogen peroxide in mitochondria is low, and can detect the mitochondria. According to the present invention, the hydrogen peroxide probe is used for detecting the hydrogen peroxide concentration in the mitochondria, the continuous fluorescence response is generated when the hydrogen peroxide concentration in the mitochondria is increased, the qualitative and quantitative analysis of the hydrogen peroxide level in the mitochondria is achieved, and the hydrogen peroxide probe can target the increased lipid droplet migration after the oxidative stress is caused by the increase of the hydrogen peroxide concentration so as to well detect the oxidative stress process; the method has important significance for exploring related damage of cell oxidative stress.
Owner:WEST CHINA HOSPITAL SICHUAN UNIV

Method of analyzing a mixed fluorescence response of a plurality of fluorophores, fluorescence analyzer, fluorescence microscope and computer program

A method of analyzing a mixed fluorescence response of a plurality of fluorophores in a microscopic sample includes reconstructing individual fluorescence responses from a mixed fluorescence response using spectral un-mixing based on reference emission spectra for fluorophores to be reconstructed, and a procedure for determining and validating reference emission spectra including providing a plurality of image acquisition settings for a sequence of images of the sample equal to, or greater than, the plurality of fluorophores and including an illumination setting for each image, acquiring the sequence of images using the plurality of image acquisition settings and storing each image together with the corresponding illumination setting, determining candidate reference emission spectra for the fluorophores to be reconstructed from the sequence of images of the sample using one or more reference emission spectra determination algorithms, and conditionally using the candidate reference emission spectra as the reference emission spectra in the spectral un-mixing.
Owner:LEICA MICROSYSTEMS CMS GMBH

Integrated micro-fluidic chip device and system for microorganism enrichment and detection

The invention relates to an integrated micro-fluidic chip device and system for microorganism enrichment and detection, the integrated micro-fluidic chip device comprises a micro-fluidic chip, the micro-fluidic chip comprises a micro-channel, the micro-channel comprises a liquid inlet and a liquid outlet, and the micro-channel allows to-be-detected liquid to flow; the micro-channel comprises a detection section; the filter membrane layer covers the inner wall of the detection section, and the filter membrane layer is used for capturing and screening microorganisms with specific sizes; the laser irradiates exciting light to the detection section, so that the specific microorganisms in the detection section are subjected to fluorescence reaction; the receiving assembly and the laser are arranged on the upper side and the lower side of the micro-fluidic chip respectively, and the receiving assembly is used for receiving fluorescence generated by the fluorescence reaction in the detection section so as to evaluate the content of microorganisms in the to-be-detected liquid. The filter membrane layer is arranged in the detection section to capture the microorganisms, so that the content of the microorganisms in the detection section is increased, the detection efficiency is improved, and the sensitivity and accuracy during low-concentration detection are improved.
Owner:SHENZHEN INST OF ADVANCED TECH

Heterojunction fluorescent probe as well as preparation method and application thereof

The invention provides a heterojunction fluorescent probe as well as a preparation method and application thereof. The heterojunction fluorescent probe comprises a Ti < 3 > C < 2 > T < x > MXene nanosheet and GQDs, wherein a heterojunction is formed between the Ti < 3 > C < 2 > T < x > MXene nanosheet and the GQDs. The 2D / 0D heterojunction fluorescent probe with the Schottky barrier can generate fluorescence response to H2S volatilization content change in the storage and decay process of fresh food, and the quality of the fresh food is determined according to TCTG fluorescence signal change.
Owner:BEIJING RES CENT FOR INFORMATION TECH & AGRI

Raman spectroscopy method and apparatus

A method of performing Raman spectroscopy comprising: generating wavelength shifted excitation light for exciting a Raman response from at least one sample, wherein the wavelength shifted excitation light comprises at least two excitation wavelengths; providing the wavelength shifted excitation light to the at least one sample and collecting signal light from the at least one sample; obtaining response signals from the collected signal light; processing the obtained response signals to determine a Raman response and a fluorescence response, wherein determining the Raman response uses at least one characteristic of an expected Raman response to the wavelength shifted excitation light and wherein determining the fluorescence response uses at least one characteristic of an expected fluorescence response to the shifted excitation light.
Owner:THE UNIV COURT OF THE UNIV OF EDINBURGH

Derivatives of triphenylamine and thiazole salt, fluorescent probe as well as preparation method and application of fluorescent probe

The invention relates to the technical field of fluorescence imaging, in particular to a derivative of triphenylamine and thiazole salt, a fluorescent probe as well as a preparation method and application of the fluorescent probe. The fluorescent probe disclosed by the invention is a derivative of triphenylamine and thiazole salt. The structure of the fluorescent probe contains a positive charge, and the internal environment of the mitochondria is a negative charge. When the fluorescent probe provided by the invention is used in cell imaging, the fluorescent probe can penetrate through a cell membrane to enter living cells and is adsorbed on mitochondria under the attraction of negative charges in the mitochondria. In the state, after the fluorescent probe is excited to generate fluorescence reaction based on a specific light field, fluorescence imaging of the cells can be realized, so that the internal change process of mitochondria can be observed through real-time fluorescence imaging. The fluorescent probe disclosed by the invention has relatively small cytotoxicity and relatively good photobleaching resistance, can specifically target mitochondria and can realize long-time fluorescence imaging, so that the fluorescent probe disclosed by the invention has relatively good advantages in cell imaging.
Owner:ANHUI UNIV

Preparation method of Eu-MOF based ratiometric fluorescence sensor and application of Eu-MOF based ratiometric fluorescence sensor in portable detection of natamycin

The invention belongs to the technical field of analysis and detection, and discloses a preparation method of an Eu-MOF based ratiometric fluorescence sensor and application of the Eu-MOF based ratiometric fluorescence sensor in portable detection of natamycin. The preparation method comprises the following steps: (1) dispersing 2, 5-dihydroxyterephthalic acid, pyromellitic acid and ZrCl4 in deionized water, then adding acetic acid, heating to react for a period of time, and then cooling to obtain a suspension; (2) transferring the obtained suspension into another reaction container, adding deionized water, continuously reacting, cooling after the reaction is completed, sequentially centrifuging with water, DMF (Dimethyl Formamide) and ethanol, and finally performing vacuum drying overnight to obtain an off-white solid UiO-66 (OH) 2 / (COOH) 2; and (3) dispersing EuCl3. 6H2O and the obtained UiO-66 (OH) 2 / (COOH) 2 in deionized water, carrying out heating reaction, cooling after the reaction is completed, centrifuging the obtained product, washing with water and ethanol, and finally, carrying out vacuum drying on the generated precipitate overnight to obtain an off-white solid Eu-coated UiO-66 (OH) 2 / (COOH) 2. The obtained Eu (at) UiO-66 (OH) 2 / (COOH) 2 has the advantages of high sensitivity, high selectivity, ratio fluorescence response and the like on NAT.
Owner:HUBEI UNIV OF TECH

Graph mode generation method based on Clarii graph

The invention discloses a graph pattern generation method based on a Clarii graph, and relates to the technical field of information, and the method comprises the steps: constructing an extended fluorescent material calibration module, introducing a saturation critical light intensity reference value, and carrying out the calibration of the Clarii graph under the dual constraints of color temperature deviation distribution and brightness standard deviation; according to the method, the pattern contrast change condition is recognized through the adjusted pattern contrast, a correction index is formed, the LED backlight wavelength is corrected for the second time according to the correction index, pattern structure deviation information is fed back to a spectrum control link, and the color uniformity of the LED backlight is improved. According to the scheme, on the premise of ensuring uniform fluorescence response and inhibiting bright spots, dark spots and stripes, the Clariy pattern can be kept stable, clear and layered in an actual application environment.
Owner:GUANGZHOU ZONGFENG NETWORK TECH CO LTD

Rare earth doped perovskite quantum dot glass and preparation method thereof

The invention relates to rare earth doped perovskite quantum dot glass and a preparation method thereof, and belongs to the technical field of inorganic functional materials and optical functional glass. The method comprises the following steps: preparing nanopore SiO2-Al2O3 glass as a matrix by adopting a sol-gel method, adding a lead source and a rare earth europium source, drying and sintering to obtain uniformly-doped porous glass, soaking the uniformly-doped porous glass in a CsBr methanol solution, and generating the CsPbBr3 perovskite quantum dots in situ. In the glass, the CsPbBr3 quantum dots show wavelength-adjustable broadband luminescence, the Eu < 3 + > ions show narrow-band luminescence, and the CsPbBr3 quantum dots and the Eu < 3 + > ions show double-peak reverse fluorescence response under the action of hydrogen chloride gas. The aperture structure can be controlled by adjusting the use amount of the aluminum lactate, so that the size and the luminescence property of the quantum dot and energy transfer between the quantum dot and Eu < 3 + > ions are adjusted, and the luminescence property and the response characteristic are optimized. The material is uniform in doping, stable in structure and suitable for the fields of multiband luminescent devices and gas sensing.
Owner:SHANGHAI INST OF OPTICS & FINE MECHANICS CHINESE ACAD OF SCI

Wound care image analysis using a smartphone

Techniques for wound image processing are disclosed. Access to a smartphone that includes an integrated color camera is obtained. An external light source is coupled to the smartphone. The external light source emits at least one light wavelength for illuminating a material sample. The light wavelength excites a fluorescence response from the sample. The fluorescence response comprises wavelengths along the Red-Green-Blue (RGB) light spectrum. The external light source is triggered. An illumination signature of the sample is captured, by the integrated color camera, in response to the external light. An output indicative of biophysical status of the sample is generated. The output is based on analysis of the captured illumination signature. A thermal image of the sample is further captured, using an infrared sensor coupled to the external light source. The output is augmented based on an analysis of the thermal image.
Owner:PRECISION HEALING INC