101 results about "Fluorescence response" patented technology

The invention discloses an indole-benzothiadiazole derivative as well as a preparation method and application thereof. The general structural formula of the indole-benzothiadiazole derivative provided by the invention is shown in the specification, wherein R is selected from one of methyl piperazinyl, diethylamino, azetidinyl and pyrrolidinyl. The derivative can be specifically combined and fluorescently labeled with mitochondrial RNA in cells, and has good selectivity, high fluorescence signal-to-noise ratio and excellent anti-interference capability in a complex biological system. Meanwhile, the indole-benzothiadiazole derivative provided by the invention is simple in synthetic route, easily available in raw materials, good in stability and convenient to store and use, can be specifically combined with RNA and generate strong fluorescence response, is suitable for real-time detection and imaging of in-vitro and in-vivo mitochondrial RNA, and is an efficient mitochondrial RNA micromolecular fluorescent probe.

The invention discloses a graph pattern generation method based on a Clarii graph, and relates to the technical field of information, and the method comprises the steps: constructing an extended fluorescent material calibration module, introducing a saturation critical light intensity reference value, and carrying out the calibration of the Clarii graph under the dual constraints of color temperature deviation distribution and brightness standard deviation; according to the method, the pattern contrast change condition is recognized through the adjusted pattern contrast, a correction index is formed, the LED backlight wavelength is corrected for the second time according to the correction index, pattern structure deviation information is fed back to a spectrum control link, and the color uniformity of the LED backlight is improved. According to the scheme, on the premise of ensuring uniform fluorescence response and inhibiting bright spots, dark spots and stripes, the Clariy pattern can be kept stable, clear and layered in an actual application environment.

The application belongs to the field of explosive detection, and discloses a polyimide loaded triaminophthalocyanine probe, a preparation method thereof and application thereof in detecting nitroaromatic explosives. The probe has the following general structure formula. The probe is prepared by cross-linking copolymerization of 5,10,15-tri(4-aminophenyl)-20-phenyl porphyrin, aromatic tetracid dianhydride and aromatic organic diamine. The polyimide high-molecular cross-linked branch loaded triaminophthalocyanine compound with fluorescent light emitting performance is prepared for the first time. The probe is applied to detecting various nitroaromatic explosives by quenching fluorescence response, and the detection method is simple, and has good application prospect in the field of public security.

The invention discloses a multi-emission composite nano ratiometric fluorescent probe for detecting fluoroquinolone antibiotics and a preparation method of the multi-emission composite nano ratiometric fluorescent probe. The CdTe-Tb-UMP composite nano ratiometric fluorescent probe with double ligands is prepared by taking metal terbium ions (Tb < 3 + >) as central ions and respectively taking uridine monophosphate (UMP) and CdTe quantum dots with red fluorescence emission as bridged ligands. ENR is further coordinated with Tb < 3 + >, energy is transferred to Tb < 3 + > through an antenna effect, so that characteristic green fluorescence of Tb < 3 + > is turned on, and fluorescence of CdTe quantum dots is almost kept unchanged. On the basis, a portable nursing point testing system is further constructed, and the portable nursing point testing system comprises an intelligent mobile phone detector and a wearable fingertip sensor integrated with a micro-fluidic chip and is used for on-site rapid detection of the ENR. The paper-based chip shows rapid and sensitive fluorescence response, and the detection limits of the spectrograph and the paper-based sensor on the ENR are 4.7 nM and 13.1 nM respectively. The probe can be applied to detection of ENR in actual environment water samples, food and drugs.

The application discloses a three-channel fluorescence sensor array based on sulfur quantum dots and a preparation method and detection application thereof. The sensor array is composed of three kinds of sulfur quantum dots, namely C-SQDs, PSS-SQDs and beta-SQDs. When target objects exist, the three kinds of sensing units generate differentiated fluorescence responses, forming a unique "fingerprint map". By using principal component analysis to analyze the response mode, the array can successfully distinguish between structurally similar baicalein, fiscetin, myricetin, quercetin and rutin in a concentration range of 8.0-56 muM. In addition, the array can also perform semi-quantitative analysis on a single flavone compound in a range of 4.0-56 muM, and the first principal component has a good linear relationship with the logarithm of the concentration of the target object.

This application discloses an embedded whole-cell biosensor, its fabrication method, and its application in machine learning-assisted mercury ion detection, belonging to the field of sensor materials and detection technology. This sensor encapsulates *E. coli* expressing green fluorescent protein (GFP) in a polyacrylamide / hyaluronic acid (PAAM / HA) hydrogel matrix, enabling highly selective and sensitive detection of mercury ions. It effectively eliminates interference from competing metal ions and can be completely regenerated through EDTA-2Na treatment, allowing for repeated use without performance loss. It maintains a stable fluorescence response for up to three weeks, possesses excellent biocompatibility and biodegradability, minimizing the risk of secondary contamination, and facilitates rapid on-site analysis.

The present disclosure relates to a diagnostic cartridge for immunodiagnosis, and a reader and a diagnostic system using the same. A diagnostic cartridge, including the diagnostic pad and the color reaction pad, is provided to detect a color reaction and a fluorescence reaction through the single diagnostic cartridge.

This invention discloses a eutectic material with ion-stimulated fluorescence response and its preparation method. The eutectic material is a co-crystallized compound formed by a fluorescent cyclophenone and a crown ether through a host-guest interaction. The eutectic material and its preparation method provided by this invention utilize a fluorescent cyclophenone as the host and a crown ether as the guest, and the two are combined through a host-guest interaction to obtain the eutectic material. This material not only possesses ion-stimulated fluorescence response characteristics but also has advantages such as high spatiotemporal resolution and good sensitivity.

This utility model relates to the field of microbial detection technology, and more specifically, to a temperature-controlled handheld ATP fluorescence detector. It includes a housing, a temperature control component, and a main circuit board and a detection mechanism respectively disposed within the housing. The detection mechanism has a detection chamber for holding a swab. The main circuit board integrates a central processing module and a fluorescence detection module. The temperature control component includes a heating element and a temperature sensor respectively installed in the detection mechanism. The signal output terminal of the temperature sensor is electrically connected to the central processing module, the signal output terminal of the central processing module is electrically connected to the heating element, and the signal output terminal of the fluorescence detection module is electrically connected to the central processing module. The temperature sensor senses the temperature of the reagent. If the detected reagent temperature is lower than the optimal temperature range for the ATP fluorescence reaction, the heating element will activate to heat the reagent to the optimal temperature range. This ensures detection accuracy even in low-temperature environments and provides high environmental adaptability.

The invention belongs to the technical field of coal mine safety monitoring, and discloses a goaf coal spontaneous combustion hidden fire source fluorescent nanoparticle dynamic real-time temperature sensing detection method, which is based on the temperature-sensitive fluorescence characteristic of fluorescent nanoparticles, and comprises the following steps of: distributing a detection medium loaded with the fluorescent nanoparticles to a goaf; a fluorescence signal acquisition device is used for receiving fluorescence response signals generated by fluorescent nanoparticles under the influence of temperature in real time, and a signal analysis system is combined for processing and analyzing characteristic parameters of the fluorescence signals, so that dynamic real-time detection of the position, the temperature and the development trend of a coal spontaneous combustion hidden fire source in a goaf is realized.

The invention relates to a preparation method and application of a sprayable surface-adaptive packaging film for in-situ detection of organophosphorus pesticides, according to the method, carboxylated cellulose nanofiber mediated defect type UiO-66-OH is used as a core functional material, sprayable slurry is prepared in cooperation with an aqueous film-forming matrix, and a continuous compact film layer is formed through a synchronous spraying process. The method is simple in processing, manufacturing tools are easy to obtain, and batch production and preparation can be realized; the packaging film obtained through the method can provide a stable and independent reaction and fluorescence response environment for in-situ detection and preliminary degradation of organophosphorus pesticides, the detection-degradation-protection integrated function is achieved, the problems that in-situ fitting performance is poor, the function is single, operation is tedious, and pollution is prone to occurring in the prior art are solved, and the packaging film has good application prospects. The paper achieves multiple functions, is suitable for detecting organophosphorus pesticide residues on the surfaces of various fruits and vegetables, and is environment-friendly, high in stability, easy to store and low in cost.

The invention discloses a method for quantitatively detecting uranyl ions by adopting a silicon-based carbon dot material and application of the method, and belongs to the field of nano composite materials and radionuclide detection. Mixing the silicon-based carbon dot composite material with an acid solution containing uranyl ions, carrying out ultrasonic treatment, detecting the fluorescence intensity of the mixed solution by adopting a fluorescence spectrum, and quantitatively analyzing the uranyl ion concentration according to the uranyl ion concentration and a fluorescence intensity curve. The silicon-based carbon dot composite material is a silicon-based carbon dot material of which the surface is modified by functional groups; the functional groups comprise hydroxyl, carboxyl and amino. The method can generate stable fluorescence response change when acting with uranyl ions, accurate quantitative analysis of uranyl ion concentration can be realized, the silicon-based carbon dot composite material has high uranyl ion detection response speed by means of the synergistic effect of the nanoscale effect and the surface functional group, and detection can be completed in a short time.

The utility model discloses a leakproofness detection device for a transformer insulating oil delivery pipeline, which comprises a pressure detection unit and an oil delivery pipeline used for installing the pressure detection unit, one end of the oil delivery pipeline is provided with a fluorescent agent injection port, and the top of the oil delivery pipeline is fixedly communicated with two groups of connecting pipes. The top ends of the two sets of connecting pipes are fixedly communicated with a U-shaped pipe, the pressure detection unit is communicated with the U-shaped pipe, the top of the oil conveying pipeline is communicated with the U-shaped pipe, a positioning detection assembly is integrated, rapid and accurate positioning of the leakage position is achieved, and when leakage happens to the oil conveying pipeline, a fluorescent agent seeps to the leakage point along with insulating oil; the ultraviolet lamp moves and irradiates along the oil conveying pipeline, the leakage position can be visually located by detecting the fluorescence reaction, the problem that in the prior art, detection only depends on the pressure attenuation rate, but the leakage point cannot be rapidly located is solved, and the detection efficiency and accuracy are remarkably improved.

The invention provides a specific probe for detecting perfluorinated compounds in a water body and a detection method thereof, and belongs to the technical field of detection. The probe provided by the invention is a compound formed by cucurbituril and fluorescein; the detection method provided by the invention comprises the following steps: adding a cucurbituril and fluorescein conjugate into a to-be-detected water sample containing the perfluorinated compounds, carrying out equilibrium reaction for a period of time, and then detecting the fluorescence response intensity in the water body so as to quantitatively evaluate the concentration of the specific perfluorinated compounds in the water sample. Compared with the prior art, the probe equipment provided by the invention is small in size, convenient to carry, capable of simultaneously detecting multiple samples and short in reaction time; the whole material of the probe is green and harmless, and chemical agents such as sulfate which easily cause secondary pollution are not used; other impurity removal and enrichment small columns and complex masking agents do not need to be used. In addition, the detection method provided by the invention has a lower detection limit, the sensitivity and convenience of novel perfluorinated compound detection are remarkably improved, and the application range is wider.

This invention discloses an electrochemical-fluorescence dual-mode biosensor for the detection of bladder cancer-related miRNA biomarkers and its application. The sensor is a homogeneous biosensor comprising a CRISPR / Cas13a system for specific recognition of target miRNAs, magnetic beads, multiple nucleic acid substrates, and one or more signal probes. The CRISPR / Cas13a system includes the Cas13a protein and crRNA with a complementary sequence to the miRNA. The magnetic beads bind to nucleic acid substrate 1, serving as the reaction substrate. After activation by the target miRNA, the CRISPR / Cas13a system trans-cleaves the ribonucleotide sites in the nucleic acid substrate 1 sequence bound to the magnetic beads. Magnetic separation of the magnetic beads and supernatant forms a dual-sensing circuit. The dual-sensing circuit reacts with different substrate nucleic acids and signal probes respectively, outputting electrochemical and fluorescence response signals, achieving single-shot dual-mode detection of the target miRNA.

A method for rapidly detecting and identifying food-borne pathogens by using a two-dimensional / zero-dimensional MXene / GQDs Schottky heterojunction fluorescence sensor array based on machine learning comprises the following steps: synthesizing three nitrogen-doped graphene quantum dots through a hydrothermal method, determining the optimal excitation wavelength, concentration, reaction temperature and pH value of the nitrogen-doped graphene quantum dots, and further determining the fluorescence intensity of the nitrogen-doped graphene quantum dots under the optimal condition; constructing a fluorescence sensor array, mixing the three graphene quantum dots with target heavy metal ions, incubating, then measuring fluorescence intensity to obtain fluorescence response data, and normalizing the data to obtain the fluorescence response data. And analyzing by using machine learning methods of a decision tree, a support vector machine, K nearest neighbor, linear discriminant analysis, naive Bayes and a neural network. The prepared fluorescence sensor array can rapidly identify heavy metal ions, is excellent in accuracy, sensitivity and stability, provides an economical and efficient solution for heavy metal ion detection, and has remarkable application potential in environmental monitoring.

The application relates to the field of optical measurement, and discloses a micropore detection assembly and method. The assembly comprises a light source for emitting excitation light, which is used for irradiating a micropore of an organic plate material, so that the organic material on the side wall and the bottom of the micropore generates a fluorescent reaction and emits fluorescence; a dichroic mirror which is arranged opposite to the light source and is used for reflecting the excitation light so that the excitation light propagates along the optical axis of a light converging element and is transmitted; a light converging element which is arranged on the side of the dichroic mirror close to the organic plate material and is used for converging the excitation light reflected by the dichroic mirror; the converged excitation light is vertically incident to the micropore; and an image sensor which is arranged on the side of the dichroic mirror far away from the organic plate material and is used for receiving the fluorescence transmitted through the dichroic mirror and generating an image including the micropore. The excitation light is used for making the inside of the micropore emit fluorescence, so that the image definition is improved, the images of the top and the bottom of the micropore are clear, and the detection efficiency is improved. In addition, residues in the micropore can also be identified.

The invention belongs to the technical field of fluorescent probes, and particularly relates to a preparation method and property research of a dual-response fluorescent probe for detecting polarity and peroxynitrite. The preparation method of the fluorescent probe is simple, the product yield is high, and the fluorescent probe is suitable for large-scale popularization and application. The present invention relates to a method for preparing a probe (Z)-4-(1-cyano-2-(6-(4-(diphenylamino) phenyl)-4-phenyl-4H-dithieno [3, 2-b: 2 ', 3'-d] pyrrol-2-yl) vinyl)-1-(4-(4, 4, 5, 5-tetramethyl-1, 3, 2-dioxaborolan-2-yl) benzyl) pyridine-1-onium bromide (DTBP). When excitation is carried out at 600 nanometers, the fluorescence intensity is gradually enhanced along with the reduction of the polarity of the solution; in a solvent of PBS and acetone in equal proportion, when excitation is carried out at 470 nanometers, the probe reacts with ONOO to generate strong fluorescence. Along with the increase of the ONOO concentration, the fluorescence intensity is gradually enhanced. The probe can specifically detect ONOO, and has a good linear relationship. The probe also has relatively strong fluorescence response to polarity, and the fluorescence emission intensity is gradually increased along with the reduction of the polarity.

The invention relates to a concentration measurement method, device and system and electronic equipment. The method comprises the following steps: acquiring the transmitted light intensity of light emitted by a light source after passing through a solution; acquiring fluorescence spectrum information emitted after the solution is irradiated by the light emitted by the light source; determining all aromatic hydrocarbon compounds contained in the solution based on the fluorescence spectrum information; based on the determined aromatic hydrocarbon compounds contained in the solution, sub-matrixes corresponding to the aromatic hydrocarbon compounds contained in the solution are extracted from a pre-stored fluorescence response matrix, and a single sub-matrix represents fluorescence response coefficients of one aromatic hydrocarbon compound in the solution under different concentrations; and determining a first concentration of each target substance in the solution based on the fluorescence intensity, the transmission light intensity and a preset concentration calculation model. In this way, the inner filtration effect between substances in the solution can be effectively resisted, and the accuracy of first concentration measurement of the target substance is improved.

The invention discloses a coal rock extract spectral analysis method, a coal rock extract spectral analysis device, a storage medium and equipment. The method comprises the following steps: refrigerating an extract of a coal rock sample and sealing and storing the extract in a glass slide; performing full-spectrum laser scanning on a specified area of the glass slide to obtain a spectrum scanning plate; on the basis of the spectrum scanning plate, obtaining an excitation wavelength and a receiving wavelength which enable the extract of the coal rock sample to be subjected to fluorescence reaction, and determining an excitation wavelength and a receiving wave band of each hydrocarbon component; the method comprises the following steps: performing multi-spectrum laser scanning imaging on all areas of a glass slide of a coal rock sample extract to obtain a laser scanning imaging image of each hydrocarbon component; and obtaining a spectral signal of each hydrocarbon component from the laser scanning imaging image of each hydrocarbon component, and obtaining the content of each hydrocarbon component in the coal rock extract based on the spectral signal. The method can accurately, rapidly and quantitatively analyze the hydrocarbon component content in the coal rock sample extract and estimate the resource quantity in the coal rock.

The present application relates to a human BMP3 and NDRG4 gene methylation detection kit, the kit comprises a restriction enzyme reaction solution, the restriction enzyme reaction solution comprises a restriction enzyme buffer, a methylation-dependent restriction enzyme, and further comprises a fluorescent PCR reaction solution of primers and a partial double-stranded linear DNA probe designed for a CpG island of a BMP3 gene and / or a NDRG4 gene. The detection kit can realize the sequential restriction enzyme reaction and fluorescent PCR reaction in one tube to detect the methylation state of the BMP3 and NDRG4 genes, and has the characteristics of simple and rapid operation, high sensitivity, good specificity and easy automation.

The invention discloses environment-friendly multifunctional gradient response allochroic silicagel and a preparation method thereof, and belongs to the technical field of allochroic silicagel. The allochroic silica gel comprises a silica gel substrate with a mesoporous-microporous two-stage pore channel structure, an organic-inorganic hybrid fluorescent allochroic indication system loaded on the inner wall of a pore channel of the substrate, and a super-hydrophobic protection layer covering the surface of a particle. According to the invention, a two-stage pore channel structure is constructed by adopting a double-template-agent synergistic self-assembly method, water molecule transmission is accelerated by utilizing a mesoporous channel, and high adsorption capacity and quick response are realized by virtue of a synergistic effect of adsorption sites provided by a microporous structure. The organic indicator is covalently anchored to a silica gel skeleton by adopting the silane coupling agent, so that dual humidity indication of visible light high-contrast discoloration and fluorescence multi-stage gradient response is realized. A super-hydrophobic protection layer is constructed on the particle surface through a fluidized bed vapor deposition method, and the intelligent protection effect of air permeability and water impermeability is achieved. The product does not contain harmful substances such as cobalt, and is environment-friendly, safe and excellent in cycling stability.

This invention provides a supramolecular fluorescence sensing array and its application in detecting five tyrosine kinase inhibitors, belonging to the field of analytical chemistry detection technology. The supramolecular fluorescence sensing array is composed of three supramolecular fluorescent probes: 2NR@Q[8], 2AO@Q[8], and APFG@Q[8]. This invention uses a supramolecular fluorescence sensing array composed of three supramolecular fluorescent probes: 2NR@Q[8], 2AO@Q[8], and APFG@Q[8]. By utilizing the cross-fluorescence response signals generated by this supramolecular fluorescence sensing array for five TKIs, combined with classical pattern recognition algorithms, it is possible to perform qualitative and quantitative detection of five TKIs: GEF, EH, LD, AD, and DAC. Moreover, this supramolecular fluorescence sensing array has good anti-interference properties, and serum samples have demonstrated that the supramolecular fluorescence sensing array constructed in this invention can simultaneously analyze five TKIs in complex systems. Therefore, this invention provides a new method for detecting tyrosine kinase inhibitors, which is more intuitive and convenient than traditional detection techniques.

The invention relates to the technical field of pathogen optical detection, and discloses a multiple immunofluorescence analysis method for detecting multiple microbial pathogens. The method comprises the following steps: acquiring multi-modal fluorescence data of a sample surface through a bionic visual perception system, and generating a pathogen surface distribution heat map by using a deep neural network to identify a high-risk area. The system dynamically regulates and controls an optical detection array to emit a coding light signal into a sample according to a region division result, and reconstructs an internal three-dimensional structure of a pathogen by analyzing fluorescence response. Surface and internal data are integrated through a space-time alignment mechanism, fusion analysis is carried out by adopting a graph neural network, and a detection confidence score is generated to drive an adaptive decision. According to the method, accurate and dynamic detection of pathogens from the surface to the interior is realized, and the detection depth and the result reliability of a hidden target in a complex sample are improved.

The embodiment of the invention provides cleaning equipment and a control method thereof. The cleaning device comprises an ultraviolet lamp source, a camera and a processing assembly. And the outer lamp source is used for emitting ultraviolet light to the stains so as to excite the fluorescence reaction of the stains. The camera is used for acquiring a fluorescence picture after the stain is subjected to fluorescence excitation reaction. And the camera is also used for acquiring a color picture of the stain. In this way, the color picture can obtain two-dimensional information such as the color, the texture and the range of the stains. Based on the biological properties of urine, the ultraviolet lamp source emits ultraviolet light to urine stains to excite fluorescence reaction of the urine, and the camera obtains fluorescence pictures of the stains. And the fluorescent picture can acquire information about whether the stain has fluorescence or not. Therefore, the processing component can determine whether the type of the liquid is urine or not through the color picture and the fluorescence picture.

The invention relates to a DMD true gray projection method and device based on fluorescent material integral transformation, and the method comprises the steps: a light source generates an excitation light beam, and the excitation light beam is projected to the surface of a DMD after being shaped; in a gray scale coding period, the DMD controls the switching time proportion of the micromirror unit according to the pixel gray scale value of an input image so as to modulate the input image into a pulse light beam carrying pixel gray scale information; converting the modulated pulse light beam into a continuously distributed true gray fluorescent light beam by using the light intensity integral effect of a fluorescent material; and then, the light splitting system is used for separating the generated real-gray-scale fluorescence light beam from the residual excitation light beam, the projection lens is used for collecting the real-gray-scale fluorescence light beam, and finally, a projection image with continuous gray scales is output. Compared with the prior art, by introducing the fluorescent film of which the fluorescence response time is matched with the DMD grayscale modulation period, the real grayscale display of the physical level is realized by utilizing the light intensity integral characteristic of the fluorescent film.

This invention relates to the field of electrocatalytic oxidation technology, and provides a fluorescence detection condition control system and method, comprising: a strategy library, which uses XGBoost to train strategy algorithm models for different water sample qualities and initial wavelength bands; an initialization module, which loads water quality types from the strategy library according to the detection environment and sets initial state water sample conditions; a pre-excitation module, which illuminates single-band LEDs one by one to obtain initial fluorescence response values; an adaptive detection module, which calls the strategy algorithm model and generates wavelength combinations and excitation intensity configurations based on the initial fluorescence response values; a temperature compensation module, which monitors key temperatures in real time using a temperature sensor and compensates for fluorescence errors caused by temperature drift; and an analysis report module, which outputs Cu... 2+ Concentration detection results. This invention combines a water quality preset strategy library and a temperature compensation mechanism to achieve Cu concentration detection in complex matrix environments. 2+ Fluorescence detection significantly improves the accuracy and environmental adaptability of detecting low-concentration heavy metals.

This application provides an optical detection system and an optical detection device. The optical detection system includes an excitation device, an adjustment device, a capillary separation channel device, a detection device, and a detection channel switching device. The adjustment device is used to adjust the direction of the excitation light. The capillary separation channel device is used to receive the excitation light after the direction is adjusted, and emit fluorescence after a fluorescence reaction. The detection device includes multiple interconnected detection channels and multiple sets of detectors. Fluorescence passes through the multiple detection channels and enters the multiple sets of detectors. The detection channel switching device is rotatably disposed at any position in the optical path formed by the excitation light and fluorescence. The detection channel switching device is provided with multiple light-transmitting parts for the excitation light or fluorescence to pass through. Each detection channel corresponds to one or more light-transmitting parts. The detection channel switching device is used to close the connection between other detection channels and other light-transmitting parts when any detection channel is connected to the corresponding light-transmitting part.

The invention discloses a fluorescent probe for detecting the viscosity of a cell membrane as well as a preparation method and application of the fluorescent probe, and belongs to the technical field of small molecule probes. The structural formula of the fluorescent probe is shown in the specification. The preparation method specifically comprises the following steps: (1) reacting 4-diethylaminoketonic acid and cyclohexanone in concentrated sulfuric acid, pouring into crushed ice, stirring, adding perchloric acid, stirring, standing and filtering; (2) dissolving 4-diethylaminobenzaldehyde in absolute ethyl alcohol, heating and refluxing, removing the solvent, and carrying out chromatography; and (3) dissolving benzotriazole-N, N, N ', N'-tetramethylurea hexafluorophosphate and N, N-diisopropylethylamine in N, N-dimethylformamide, stirring at normal temperature, adding a compound 43-(tetradecyl amino) propane-1-sulfonate, continuously stirring, removing the solvent, and carrying out chromatography to obtain the compound 43-(tetradecyl amino) propane-1-sulfonate. The fluorescent probe has excellent anchoring and imaging capabilities and viscosity-triggered near-infrared (NIR) fluorescence response characteristics, can be used for fluorescence imaging of cell membrane viscosity, and can also be used for viscosity imaging analysis of breast cancer mice.

The present application relates to a kind of kits for detecting sows' nutritional iron deficiency by biomarker quantitative detection evaluation and application, including fluorescent reaction liquid A, fluorescent reaction liquid B, internal standard, negative control, nucleic acid release agent and quantitative reference product, the fluorescent reaction liquid B contains detection primer and probe, the detection primer includes upstream primer and downstream primer, the sequence of upstream primer is 5'-ATCTGGCTTGTAAACCAACA-3', the sequence of downstream primer is 5'-ATCTTAGTTGTGGATTCCGT-3', the fluorescent reaction liquid B also contains primer probe, probe sequence is 5'-TCACCGTCAATTTCGGCTCT-3', 5' end is labeled with reporter fluorescent group, and 3' end is labeled with quenching fluorescent group.The present application effectively improves the accuracy of sows' nutritional iron deficiency detection, and provides scientific guidance for sow nutrition.