411 results about "Fluorescence response" patented technology

A method of monitoring residual treating agent in treated water wherein fluorescent tracers are used to determine the concentration of treating agent in the water at two different treating agent dosages and the difference in the measured concentrations of the fluorescent tracer at the respective doses are correlated with the residual concentration of the treating agent. The fluorescence response at the different treating agent dosages is used to determine whether the system is overdosed or underdosed on a continuous basis and to control treating agent dose accordingly.

The invention discloses an integrated micro-fluidic chip system, which comprises a micro-fluidic chip, a light source unit, a sample introduction unit, a detection unit and a communication unit, wherein the micro-fluidic chip is connected with the light source unit through optical fibers and connected with the sample introduction unit through a hose; and the communication unit is electrically connected with the detection unit, the light source unit and the sample introduction unit. When used for detecting biochemical substances having a fluorescence response property in a fluid sample, the system can solve the problems in prior art such as time consumption, large reagent and sample consumption, complex operation and the like, can realize fluorescence detection under various conditions, hasthe advantages of high integration, quick analysis, high flexibility, little sample consumption, simple operation, quantitative analysis, online real-time detection and the like, has a compiling control degree that allows for combined use with a computer, independent operation, and can be used as a sensor unit in a wireless sensor network.

In a sensor system, an active sensor chip includes an array of periodically-patterned dielectric active sensor patches of different periodicities and geometries formed on a metal film. A specimen under study is positioned on each patch, and the active sensor chip is interrogated by illumination the patches in a predetermined sequence to result in a fluorescence response from each patch enhanced by SPP. The intensity of the fluorescence response is controlled by varying the wavelength, incidence angle, azimuthal orientation and polarization direction of the excitation light beam as the function of the periodicity of the illuminated patch. The system is compatible with commercial fluorescence microscopes and scanned laser interrogation systems.

A method of monitoring residual treating agent in treated water wherein fluorescent tracers are used to determine the concentration of treating agent in the water at two different treating agent dosages and the difference in the measured concentrations of the fluorescent tracer at the respective doses are correlated with the residual concentration of the treating agent. The fluorescence response at the different treating agent dosages is used to determine whether the system is overdosed or underdosed on a continuous basis and to control treating agent dose accordingly.

A method and a system is provided for discriminating between healthy cervical tissue and pathologic cervical tissue based on the fluorescence response of the tissue to laser excitation (LIF) and the backscatter response to illumination by white light (in the spectral range of 360 to 750 nm). Combining LIF and white light responses, as well as evaluating a spatial correlation between proximate cervical tissue sites in conjunction with a statistically significant “distance” algorithm, such as the Mahalanobis distance between data sets, can improve the discrimination between normal and abnormal tissue. The results may be displayed in the form of a map of the cervix representing the suspected pathology.

The invention relates to a synthesis method and application of a ratiometric fluorescent molecular probe for simultaneously detecting a fluorine ion and a sulfite radical. The ratiometric fluorescent molecular probe adopts a 2-(2-hydroxyphenyl)benzothiazole derivative as a matrix structure, and detects the fluorine ion and the sulfite radical based on excited-state intramolecular proton transfer (ESIPT) and intramolecular charge transfer (ICT) mechanisms, respectively. The probe has a maximum emission wavelength of 498 nm in an acetonitrile solution with a concentration of 80%, when the fluorine ion is added, the fluorescence spectrum of the probe has a red shift of 136 nm; and when the sulfite radical is added, the fluorescence spectrum of the probe has a blue shift of 127 nm. The differentiated detection of the two ions can be realized by the fact that the fluorescence spectrum of the probe has an obvious red shift or blue shift after the fluorine ion or sulfite radical is added, respectively, showing different fluorescence response signals. The inventive fluorescent probe has the advantages of simple operation, mild reaction conditions, easy purification, high synthesis yield, good selectivity, high sensitivity and stable optical performances. At the same time, the design and synthesis of the fluorescent probe provide an important foundation for development of multi-functional fluorescent probes in the future.

The invention discloses a primer and probe for detecting a human epidermal growth factor receptor (EGFR) gene and / or a K-ras gene, a kit containing the primer and the probe and a device for detecting genetic mutation on the basis of a digital PCR platform.The method for detecting the genetic mutation by means of the primer and the probe comprises the steps that the prime and the probe are provided; DNA of a sample to be detected is extracted; a fluorescent PCR reaction system capable of amplifying a mutant gene sequence is prepared; a target probe and an internal reference probe are utilized to be hybridized with amplified products respectively, and fluorescent signals of corresponding fluorescent groups are detected; existence of the genetic mutation is judged and / or the mutation rate is calculated according to the strength and proportion of the fluorescent signals of the target probe and the internal reference probe.According to the method for detecting the genetic mutation, the needed primers and probes are small in number, the optimization procedure is simple, related mutation of EGFR and / or K-ras gene can be qualitatively or quantitatively detected, and the detection sensitivity is high; a DNA sample with low initial amount can also be detected stably.

The invention relates to one-dimensional organic semiconductor spiral nano-wires with ultra-sensitive fluorescence response upon organic amine gases, and a preparation method and an application thereof. According to the invention, perylene imide derivatives comprising perylene and with asymmetric amphiphilic substituents on two ends are adopted as construction units; in a mixed liquid of a good solvent and a poor solvent, through pi-pi interactions between perylenes of a plurality of perylene imide derivatives comprising perylene and with asymmetric amphiphilic substituents on two ends, the one-dimensional organic semiconductor spiral nano-wires are obtained through self-assembly. The one-dimensional organic semiconductor spiral nano-wires have two significant advantages of a nano-grade spiral structure and good fluorescence quantum yield (up to 25%), such that the nano-wires are suitable to be used in fluorescence detection of organic amine in air. When a network-structured porous membrane woven by using the one-dimensional organic semiconductor spiral nano-wires contacts trace amine vapour (with a concentration of ppb-ppm level), the fluorescence is quenched.

The invention discloses a preparation method and application of a ratiometric double fluorescence probe. CdTe quantum dot and Au nano-cluster are taken as a fluorescence signal source, a silicon-based material is taken as a carrier, the quantum dot is taken as a reference signal source and is embedded in a silicon core, and the Au NCs (Au nano-cluster) with fluorescence response performance is covalently bonded to the surface of a silicon layer, so that a hybrid microballoon with the core-satellite type structure is prepared. The advantages comprise that the quantum dot is taken as the reference signal source and is embedded into the silicon core, leakage is effectively avoided, a stable reference signal is provided; and by covalently bonding the Au NCs with the fluorescence response performance to the silicon ball surface, selective identification and response of Cu<2+> are realized.

Methods of monitoring residual treating agent in treated water wherein the fluorescence intensity of the water at at least two different dosages of treating agent tagged or traced with fluorescent tracers are correlated with the residual concentration of treating agent. The fluorescence response at the different treating agent dosages is also used to automatically determine an optimal treating agent dose on a continuous basis and to control treating agent dose accordingly.

The invention discloses a fluorescent block-grafted copolymer with temperature sensitivity and a preparation method and application thereof. According to the fluorescent block-grafted copolymer and the preparation method and application of the fluorescent-block-grafted copolymer, an acrylate monomer containing a fluorophore is copolymerized with methacrylic glyceride through atom transfer radical polymerization to introduce an azide group to an epoxy group on the side chain, and then, a polymer with temperature response is grafted to the copolymer through clicking chemical reaction so as to form the block-grafted copolymer; and the reaction conditions are mild, and the conversion rate is high. The fluorescent block-grafted copolymer and the preparation method and application of the fluorescent block-grafted copolymer have the advantages that the polymer with temperature response is initiatively polymerized with the side chain of the copolymer with a fluorescence effect through grafting, so that the macromolecular polymer not only has fluorescent characteristics, but also the temperature response, side chain length and hydrophilicity strength of the macromolecular polymer can be controlled through adjusting the length of hydrophilic side chains and grafting efficiency, and the controllable adjustment is realized; and the structure of the copolymer is provided with hydrophilic chain segments, so that not only can kernel fluorescent chromophores be protected, but also the chromophores can be gathered together, thereby improving fluorescence response sensitivity.

The invention discloses an organic ligand for synthesizing metal organic frames. The chemical nomenclature of the organic ligand is 2-(3, 5-bis (4-pyridyl) phenyl) benzoglioxaline. The chemical formula of the organic ligand is represented in the description. The invention also discloses a synthetic method of the organic ligand and a Cu (I) ion-based bright yellow metal organic frame (MOF-1) and a Cu (I) ion-based dark red metal organic frame (MOF-2), and the chemical formulas are {[Cu(L)I].0.5CH3CN.0.5CH3OH.0.75H2O}n, (n=1,2,3...'8') and {[Cu(L)I].2H2O}n(n=1,2,3...'8'). The invention also provides a synthetic method for dark red Cu (I) ion metal organic frame (MOF-2) and a synthetic method for organic matters of the Cu (I) ion metal organic frame. The invention also provides application of the organic ligand and metal organic frames serving as alcohols small organic molecule fluorescence sensing materials. The application operation is simple, the cost is low, the respond is quick, and the obvious fluorescence response for methanol, ethanol, isopropanol , n-propanol under the liquid phase and the gas phase can be achieved.

A method for detecting a plurality of reactive sites on an analyte, comprising allowing reactants on an addressable microsphere and the reactive sites to react, forming reactant-reactive site pairs distinguishable by fluorescence intensity. The invention also provides a method for detecting a plurality of analytes in a sample using addressable microspheres in combination with one or more reporter reagents. Also provided are a method for determining allele zygosity of a genetic locus having two alleles or more alleles using microparticles, and a method for detecting a plurality of SNPs in nucleic acid molecules. The instant invention also provides a composition comprising an addressable microsphere carrying at least two fluorescent reactants capable of forming reactant-analyte pairs distinguishable by their fluorescence intensity, and kits comprising the inventive composition and a plurality of reporter reagents.

The invention relates to a method for detecting melamine in milk which is built based on the inner filter effect (IFE) between cysteine (L-Cys)-modified CdS quantum dots and citric acid ligand coating gold nanoparticles (AuNPs), and belongs to the field of analytical chemistry. The method comprises the detecting steps of preparing the AuNPs, synthesizing and purifying the L-Cys-modified CdS quantum dots, pre-treating a milk sample and detecting the melamine in the milk. According to the principle of recovering the fluorescence intensity of the CdS quantum dots by adopting the AuNPs to cause the fluorescence quenching of the CdS quantum dots and adopting the melamine to induce the aggregation of the AuNPs, the method can simply, quickly and sensitively detect the melamine in the milk so as to provide convenience to future dairy industry.

The invention discloses a planar miniature multi-channel fluorescence detection optical system which comprises a planar exciting light assembly and a planar lighting assembly; the reaction tank is respectively connected to said two assemblies through optical fibers; the planar exciting light assembly includes that single LED light source light serves as single-channel light to penetrate through adichroscope after being filtered and collimated and / or is focused to an optical fiber after being reflected by the dichroscope; the single-channel light in different directions passes through the first-stage dichroscope and then is output as a group of light beams in the same emitting direction; the light beams in different directions pass through a second-stage dichroscope and then are output asa multi-channel light beam group in the same emitting direction; the planar lighting assembly is characterized in that a fluorescence reaction passes through a cylindrical lens and then penetrates through a dichroscope and / or is reflected by the dichroscope and then is separated into a plurality of single-channel fluorescence in different directions, and the single-channel fluorescence is focusedand filtered and then is transmitted to a photosensitive surface of a photodiode; the fluorescence in the single direction passes through a second-stage dichroscope and then is output as fluorescencebeams in different emitting directions; and each group of fluorescence beams pass through the first-stage dichroscope and then are output as single-channel fluorescence in different emitting directions.

The invention discloses a series of cyano-substituted diphenylethylene type co-crystallization compounds with multi-stimulus fluorescence response property and a preparation method thereof, belonging to the field of crystalline materials with subject and object structures. The novel crystalline compounds are obtained by selecting a cyano-substituted diphenylethylene compound as a subject, taking a series of organic small molecular compounds with hydroxyl, carboxyl and other groups or halogenated benzene and the like as objects, and performing co-crystallization by subject and object identification and intermolecular interaction. The compounds disclosed by the invention are characterized in that the selected cyano-substituted diphenylethylene compound has excellent optical property, the co-crystallization materials can be obtained by the action of frictional force, the ultrasonic action, solvent volatilization and other methods, and the precise crystal structures can be determined; and simultaneously, the series of the co-crystallization materials have reversible fluorescence response against a variety of external stimuli (such as light, pressure, organic solvents and the like). The compounds have application prospects in the fields of manufacturing of optical anti-counterfeiting materials, fluorescent sensors and optoelectronic devices, and the like.

The invention belongs to the field of a functional high molecular material, and particularly relates to a series of side chain type electroactive fluorescent polymers, a preparation method and use for detecting an oxidizing or reducing substance. Fluorescence detection of the oxidizing or reducing substance is realized by utilizing the characteristic that the fluorescence intensity of the electroactive fluorescent polymer reversibly changes along with an oxidation reduction state of an aniline chain segment. The preparation method comprises the following steps of: synthesizing the fluorescentpolymer containing the aniline chain segment and a fluorescent group on a side chain through a ternary polymerization method; after preparing polymer into solution, keeping the aniline chain segment in the polymer in a reducing state or intermediate oxidizing state by using a chemical oxidation reduction method or electrochemical method; and then testing a fluorescence response situation of the aniline chain segment to the oxidizing or reducing substance by using a fluorescent spectrometer. The detection method has the advantages of wide variety of detection objects, high stability, low detection lower limit, reusability and the like, and has extremely broad application prospect in the fields of biology, chemistry, foods, environment and the like.

The invention provides a quick and high-efficiency fluorescence detection method for phosphate ions. Rare earth ions can quench fluorescence based on a quantum dot, and the fluorescence with the phosphate ions can be recovered effectively. Compared with the most common fluorescence quenching type probe based on the quantum dot, a novel off-on type quantum dot fluorescence probe can effectively prevent the fluorescence from being quenched due to other factors in a solution, so that the measurement selectivity is improved greatly. As known, the method is the first application of the quantum dot to measurement of the phosphate ions. According to the method, the phosphate ions can be measured with high sensitivity; the linear range is 1*10<-7> to 5*10<-6> mol / L; and the detection limit is 5*10<-8> mol / L. Common anions do not have fluorescence response signals, so that the method is high in selectivity; a satisfying result of measurement of the phosphate ions in an analog water sample is obtained; and the method is expected to be applied to detection of the phosphate ions in a water body.

The invention provides a preparation method of an array fluorescent nano-cluster sensor and an application of the array fluorescent nano-cluster sensor to metal ion recognition. The preparation method comprises steps as follows: 1), seven fluorescent nano-clusters required by the array fluorescent nano-cluster sensor are synthesized; 2), the array fluorescent nano-cluster sensor is prepared by means of the seven fluorescent nano-clusters. A method for applying the array fluorescent nano-cluster sensor to metal ion recognition comprises steps as follows: different samples containing metal ions are mixed with the array fluorescent nano-cluster sensor; fluorescence intensity signals of the seven nano-clusters, responding to the samples, in the array fluorescent nano-cluster sensor are read; the fluorescence response signals of the seven nano-clusters responding to the samples are taken as array signals to recognize the samples; finally, a practical water sample is recognized by means of a nano-cluster array. Experiments prove that the method is convenient and efficient, metal ions in the samples can be quickly responded, and the samples containing the metal ions are quickly recognized according to the recognition response signals of the nano-cluster array responding to the metal ions.

The invention discloses a detection method for identifying the American classical PRRSV strain, the HP-PRRSV strain and a new-type viral NADC30 strain at the same time. In the method, a multiplex real-time fluorescence RT-PCR for identifying three kinds of strains at the same time is established based on an ABI7500 fluorescent quantitative PCR instrument, wherein the strains include the classical PRRSV strain, the highly-pathogenic HP-PRRSV strain with the deletion of L-amino acid at the 481st locus of the Nsp2 gene and the continuous deletion of 29 amino acids at the 533ird-561st loci of the Nsp2 gene, and NADC30like; specific primers and specific probes for the three kinds of strains are designed respectively based on the Nsp2 gene, universal primers and universal probes for PRRSV are designed according to the relatively conserved N gene, missing detection caused by Nsp2 region mutation can be effectively prevented, and false negative can be avoided.

The invention discloses a hypochlorite ion fluorescence probe, a preparation method and an application thereof. The hypochlorite ion fluorescence probe can be used for detecting hypochlorite ion content in environment, fluorography of hypochlorite distributed in a biological sample and content detection. The fluorescence probe has fluorescence response by comparing with hypochlorite, and has no reaction to other various reactive oxygen species (ROS) such as H2O2, NO<.>, <.>O2<->, ONOO<->, ROO<.>, <1>O2 and <.>OH, and has good selectivity and specificity. The preparation method of the probe has the advantages of easy and simple process, and easy large-scale production. According to the invention, a structure of the probe is shown as the following; and the preparation method is characterized in that fluorescein signal-aldehydes and hydrazinopyridines are reacted in an ethyl alcohol solution to generate the hypochlorite ion fluorescence probe.

The invention belongs to the field of fluorescence chemical sensor with nanostructure and especially relates to a silicon nano wire based chemical sensor having selective fluorescence response on nitric oxide, and a preparation method and application thereof. According to the invention, silicon nano wire and silicon nano wire array prepared from a chemical vapor deposition method and from a chemical etching method are treated with a surface treatment; then an organic micromolecule substance amino fluorescein is used to carry out covalent modification on the silicon nano wire and the silicon nano wire array to obtain the silicon nano wire based sensor having selective fluorescence response on nitric oxide. The silicon nano wire based chemical sensor having selective fluorescence response on nitric oxide can be used in detection of solution and biosystem.

The invention relates to rubber with fluorescence response to a pH value and a preparation method thereof. The method comprises the following steps of: measuring acetyl chloride, adding into rhodamine B-containing alcohol solution dropwise, preserving heat for reaction, evaporating to remove the alcohol solution under reduced pressure, and washing by using ethyl acetate to obtain rhodamine B ester; dissolving the rhodamine B ester in hot methanol, adding ethylenediamine, performing refluxing reaction, cooling to room temperature, removing the methanol, adding an organic solvent 1 and water, and washing and drying an organic layer to obtain ethylenediamine substituted rhodamine B; and weighing chlorosulfonated polyethylene rubber, swelling in an organic solvent 2, adding the ethylenediamine substituted rhodamine B, reacting, filtering off a solid, washing, and drying to obtain the rubber with the fluorescence response to the pH value. The rubber prepared by the method is functional rubber with the fluorescence response to the pH value, and can detect and analyze the pH value of a biological system, a chemical reaction system, industrial wastewater, a river, a lake or an ocean.

The present invention relates to one-dimensional organic semiconductor nano-wires capable of providing ultra-sensitive fluorescence response (ppm level) to volatile organic compound gases, a preparation method and applications thereof. According to the present invention, a unilateral alkyl chain substituted perylene bisimide derivative is used as a constructing unit, and is self-assembled in the mixed solution of a good solvent and a poor solvent to obtain the one-dimensional organic semiconductor nano-wire having good fluorescence quantum yield (at least 11%); with the nano-wire bundling film prepared from the nano-wire and having the porous mesh structure, volatile organic compound molecules are easily diffused in the film, such that the distance between the nano-wires in the bundle can be affected so as to effectively perform fluorescence detection on the volatile organic compound.

The invention belongs to the field of nanostructured fluorescent chemosensors, and especially relates to a silicon-nanowire-based alkaline phosphatase fluorescent chemosensor and a preparation method, and application of the fluorescent chemosensor. The silicon-nanowire-based alkaline phosphatase fluorescent chemosensor is a silicon-nanowire or silicon-nanowire-array fluorescent chemosensor with the surface modified by phosphorylated fluorescein molecules possessing selective fluorescence response to alkaline phosphatase. The silicon-nanowire-based alkaline phosphatase fluorescent chemosensor is applicable to detect alkaline phosphatase molecules in a solution system containing alkaline phosphatase molecules. A single-silicon-nanowire fluorescent chemosensor scraped off from a silicon-nanowire array has wide application prospect on detection of alkaline phosphatase molecules in unicell level, and helps to provide a new sensor for directly detecting the activity of alkaline phosphatase in cells.

The invention discloses a mercury ion probe and its preparation method and use. The mercury ion probe can be used as a fluorescent and colorimetric sensor of a mercury ion aqueous solution, can be prepared by simple processes, and has very good selectivity and sensitivity of mercury ion detection and very fast reaction time. A test paper strip prepared from the mercury ion probe has very high sensitivity of mercury ions, a mercury ion fluorescence response detection limit of 1*10<-6> mol / L and a mercury ion colorimetric response detection limit of 1*10<-7> mol / L (20ppb). In practical application, only by naked eyes, the test paper strip prepared from the mercury ion probe has mercury ion aqueous solution detection limit of 20ppb without other apparatuses. Through convenient and cheap preparation of the test paper strip, storage convenience and actual operation convenience, the mercury ion sensor can be developed into a practical application tool for on-site examination of mercury ion pollution.

The invention discloses a method for detecting microbial communities in sediment by a fluorescence in situ hybridization (FISH) technique. The method comprises the steps of pretreatment of a sediment sample, spread coating and dehydration of the sample, hybridization and washing, and observation with a fluorescence microscope, and detects the microbial communities in the sediment through the fluorescence responses evoked by a different detection probes. By optimizing the analysis conditions of the fluorescence in situ hybridization technique, the method reduces autofluorescence in the sample, eliminates nonspecific hybridization, and determines the hybridization times and the concentration of an eluent, thereby applying the FISH technique to detection of microbial communities in the sediment sample.

The invention relates to one-dimensional organic semiconductor nanowire / nanoribbon with ultrasensitive (ppb grade) fluorescence response to organophosphorus ester and related organic phosphorus-containing compound toxic gases and a preparation method and application thereof. The nanowire or nanoribbon is obtained by self-assembling of various perylene-containing amphiphilic perylene bisimide derivatives through Pi-Pi interaction. The perylene-containing amphiphilic perylene bisimide derivative has asymmetric amphiphilic substituent, wherein one end is a nonpolar straight-chain or branched-chain long-chain alkyl, and the other end is aryl containing polar group or aryl alkyl. According to a film formed by weaving the one-dimensional organic semiconductor nanowires or nanoribbons, detection concentration of steam of toxic organic phosphorus-containing compounds reaches the grade of several ppb to one hundred and more ppm. The product has a great application prospect.

The present disclosure concerns a method and system for imaging a molecular strand (MS). The method comprises providing a sample volume (SV) comprising the strand (MS); providing an excitation beam (EB) having an excitation focus (EF) in the sample volume (SV); scanning the excitation focus (EF) in the sample volume (SV) along a one dimensional scanning line (SL); trapping an end of the strand (MS) in the sample volume (SV) and extending the strand (MS) along a one-dimensional trapping line (LL) parallel to the scanning line (SL); aligning the trapping line (LL) to coincide with the scanning line (SL) to have the scanning excitation focus (EF) coincide with the strand (MS); and recording the fluorescence response (FR) as a function of a plurality of distinct scanning positions (X0) of the excitation focus (EF) along the scanning line (SL).

The invention relates to a phenothiazinyl quinazoline fluorescence ion probe and application thereof. A fluorescence chemical sensor is based on a phenothiazinyl-substituted quinazoline derivative, and the structural general formula of the derivative is shown as a formula (1), wherein R1 is a hydrogen atom, alkyl which contains 1 to 10 carbon atoms, ether, azyl, aliphatic amide, imide, an ester group, an aryl substituent group or a hybrid aryl substituent group. Compared with other macromolecule fluorescence probe molecules, the fluorescence ion probe has the characteristics of simplicity in dissolution and synthesis and the like, and good response and identification effects on Cu<2+>, and also has a good identification effect on Hg<2+> in the presence of Cu<2+>, and generates different fluorescence responses to Hg<2+> and Cu<2+> under different conditions, multifunctionality on ion detection, and can also be designed into ion light introducing or fluorescence inhibit (INH) logic gate.