406 results about "Fluorescence response" patented technology

The invention discloses an integrated micro-fluidic chip system, which comprises a micro-fluidic chip, a light source unit, a sample introduction unit, a detection unit and a communication unit, wherein the micro-fluidic chip is connected with the light source unit through optical fibers and connected with the sample introduction unit through a hose; and the communication unit is electrically connected with the detection unit, the light source unit and the sample introduction unit. When used for detecting biochemical substances having a fluorescence response property in a fluid sample, the system can solve the problems in prior art such as time consumption, large reagent and sample consumption, complex operation and the like, can realize fluorescence detection under various conditions, hasthe advantages of high integration, quick analysis, high flexibility, little sample consumption, simple operation, quantitative analysis, online real-time detection and the like, has a compiling control degree that allows for combined use with a computer, independent operation, and can be used as a sensor unit in a wireless sensor network.

In a sensor system, an active sensor chip includes an array of periodically-patterned dielectric active sensor patches of different periodicities and geometries formed on a metal film. A specimen under study is positioned on each patch, and the active sensor chip is interrogated by illumination the patches in a predetermined sequence to result in a fluorescence response from each patch enhanced by SPP. The intensity of the fluorescence response is controlled by varying the wavelength, incidence angle, azimuthal orientation and polarization direction of the excitation light beam as the function of the periodicity of the illuminated patch. The system is compatible with commercial fluorescence microscopes and scanned laser interrogation systems.

A method of monitoring residual treating agent in treated water wherein fluorescent tracers are used to determine the concentration of treating agent in the water at two different treating agent dosages and the difference in the measured concentrations of the fluorescent tracer at the respective doses are correlated with the residual concentration of the treating agent. The fluorescence response at the different treating agent dosages is used to determine whether the system is overdosed or underdosed on a continuous basis and to control treating agent dose accordingly.

The invention relates to a synthesis method and application of a ratiometric fluorescent molecular probe for simultaneously detecting a fluorine ion and a sulfite radical. The ratiometric fluorescent molecular probe adopts a 2-(2-hydroxyphenyl)benzothiazole derivative as a matrix structure, and detects the fluorine ion and the sulfite radical based on excited-state intramolecular proton transfer (ESIPT) and intramolecular charge transfer (ICT) mechanisms, respectively. The probe has a maximum emission wavelength of 498 nm in an acetonitrile solution with a concentration of 80%, when the fluorine ion is added, the fluorescence spectrum of the probe has a red shift of 136 nm; and when the sulfite radical is added, the fluorescence spectrum of the probe has a blue shift of 127 nm. The differentiated detection of the two ions can be realized by the fact that the fluorescence spectrum of the probe has an obvious red shift or blue shift after the fluorine ion or sulfite radical is added, respectively, showing different fluorescence response signals. The inventive fluorescent probe has the advantages of simple operation, mild reaction conditions, easy purification, high synthesis yield, good selectivity, high sensitivity and stable optical performances. At the same time, the design and synthesis of the fluorescent probe provide an important foundation for development of multi-functional fluorescent probes in the future.

The invention discloses a preparation method and application of a ratiometric double fluorescence probe. CdTe quantum dot and Au nano-cluster are taken as a fluorescence signal source, a silicon-based material is taken as a carrier, the quantum dot is taken as a reference signal source and is embedded in a silicon core, and the Au NCs (Au nano-cluster) with fluorescence response performance is covalently bonded to the surface of a silicon layer, so that a hybrid microballoon with the core-satellite type structure is prepared. The advantages comprise that the quantum dot is taken as the reference signal source and is embedded into the silicon core, leakage is effectively avoided, a stable reference signal is provided; and by covalently bonding the Au NCs with the fluorescence response performance to the silicon ball surface, selective identification and response of Cu<2+> are realized.

The invention discloses an organic ligand for synthesizing metal organic frames. The chemical nomenclature of the organic ligand is 2-(3, 5-bis (4-pyridyl) phenyl) benzoglioxaline. The chemical formula of the organic ligand is represented in the description. The invention also discloses a synthetic method of the organic ligand and a Cu (I) ion-based bright yellow metal organic frame (MOF-1) and a Cu (I) ion-based dark red metal organic frame (MOF-2), and the chemical formulas are {[Cu(L)I].0.5CH3CN.0.5CH3OH.0.75H2O}n, (n=1,2,3...'8') and {[Cu(L)I].2H2O}n(n=1,2,3...'8'). The invention also provides a synthetic method for dark red Cu (I) ion metal organic frame (MOF-2) and a synthetic method for organic matters of the Cu (I) ion metal organic frame. The invention also provides application of the organic ligand and metal organic frames serving as alcohols small organic molecule fluorescence sensing materials. The application operation is simple, the cost is low, the respond is quick, and the obvious fluorescence response for methanol, ethanol, isopropanol , n-propanol under the liquid phase and the gas phase can be achieved.

The invention discloses a planar miniature multi-channel fluorescence detection optical system which comprises a planar exciting light assembly and a planar lighting assembly; the reaction tank is respectively connected to said two assemblies through optical fibers; the planar exciting light assembly includes that single LED light source light serves as single-channel light to penetrate through adichroscope after being filtered and collimated and / or is focused to an optical fiber after being reflected by the dichroscope; the single-channel light in different directions passes through the first-stage dichroscope and then is output as a group of light beams in the same emitting direction; the light beams in different directions pass through a second-stage dichroscope and then are output asa multi-channel light beam group in the same emitting direction; the planar lighting assembly is characterized in that a fluorescence reaction passes through a cylindrical lens and then penetrates through a dichroscope and / or is reflected by the dichroscope and then is separated into a plurality of single-channel fluorescence in different directions, and the single-channel fluorescence is focusedand filtered and then is transmitted to a photosensitive surface of a photodiode; the fluorescence in the single direction passes through a second-stage dichroscope and then is output as fluorescencebeams in different emitting directions; and each group of fluorescence beams pass through the first-stage dichroscope and then are output as single-channel fluorescence in different emitting directions.

The invention discloses a series of cyano-substituted diphenylethylene type co-crystallization compounds with multi-stimulus fluorescence response property and a preparation method thereof, belonging to the field of crystalline materials with subject and object structures. The novel crystalline compounds are obtained by selecting a cyano-substituted diphenylethylene compound as a subject, taking a series of organic small molecular compounds with hydroxyl, carboxyl and other groups or halogenated benzene and the like as objects, and performing co-crystallization by subject and object identification and intermolecular interaction. The compounds disclosed by the invention are characterized in that the selected cyano-substituted diphenylethylene compound has excellent optical property, the co-crystallization materials can be obtained by the action of frictional force, the ultrasonic action, solvent volatilization and other methods, and the precise crystal structures can be determined; and simultaneously, the series of the co-crystallization materials have reversible fluorescence response against a variety of external stimuli (such as light, pressure, organic solvents and the like). The compounds have application prospects in the fields of manufacturing of optical anti-counterfeiting materials, fluorescent sensors and optoelectronic devices, and the like.

The invention belongs to the field of fluorescence chemical sensor with nanostructure and especially relates to a silicon nano wire based chemical sensor having selective fluorescence response on nitric oxide, and a preparation method and application thereof. According to the invention, silicon nano wire and silicon nano wire array prepared from a chemical vapor deposition method and from a chemical etching method are treated with a surface treatment; then an organic micromolecule substance amino fluorescein is used to carry out covalent modification on the silicon nano wire and the silicon nano wire array to obtain the silicon nano wire based sensor having selective fluorescence response on nitric oxide. The silicon nano wire based chemical sensor having selective fluorescence response on nitric oxide can be used in detection of solution and biosystem.

The present invention relates to one-dimensional organic semiconductor nano-wires capable of providing ultra-sensitive fluorescence response (ppm level) to volatile organic compound gases, a preparation method and applications thereof. According to the present invention, a unilateral alkyl chain substituted perylene bisimide derivative is used as a constructing unit, and is self-assembled in the mixed solution of a good solvent and a poor solvent to obtain the one-dimensional organic semiconductor nano-wire having good fluorescence quantum yield (at least 11%); with the nano-wire bundling film prepared from the nano-wire and having the porous mesh structure, volatile organic compound molecules are easily diffused in the film, such that the distance between the nano-wires in the bundle can be affected so as to effectively perform fluorescence detection on the volatile organic compound.

The invention discloses a mercury ion probe and its preparation method and use. The mercury ion probe can be used as a fluorescent and colorimetric sensor of a mercury ion aqueous solution, can be prepared by simple processes, and has very good selectivity and sensitivity of mercury ion detection and very fast reaction time. A test paper strip prepared from the mercury ion probe has very high sensitivity of mercury ions, a mercury ion fluorescence response detection limit of 1*10<-6> mol / L and a mercury ion colorimetric response detection limit of 1*10<-7> mol / L (20ppb). In practical application, only by naked eyes, the test paper strip prepared from the mercury ion probe has mercury ion aqueous solution detection limit of 20ppb without other apparatuses. Through convenient and cheap preparation of the test paper strip, storage convenience and actual operation convenience, the mercury ion sensor can be developed into a practical application tool for on-site examination of mercury ion pollution.

The present disclosure concerns a method and system for imaging a molecular strand (MS). The method comprises providing a sample volume (SV) comprising the strand (MS); providing an excitation beam (EB) having an excitation focus (EF) in the sample volume (SV); scanning the excitation focus (EF) in the sample volume (SV) along a one dimensional scanning line (SL); trapping an end of the strand (MS) in the sample volume (SV) and extending the strand (MS) along a one-dimensional trapping line (LL) parallel to the scanning line (SL); aligning the trapping line (LL) to coincide with the scanning line (SL) to have the scanning excitation focus (EF) coincide with the strand (MS); and recording the fluorescence response (FR) as a function of a plurality of distinct scanning positions (X0) of the excitation focus (EF) along the scanning line (SL).