180 results about "Fluorescence response" patented technology

The invention relates to the technical field of quality detection, in particular to an AI-based liquid crystal glass plate defect detection method and system, comprising multi-modal data acquisition, adaptive preprocessing, dynamic intelligent detection, defect cognition decision, closed-loop feedback execution, a safety guardrail mechanism and digital twin support. Compared with the prior art which adopts a single imaging mode for surface detection and has the defects that the omission ratio of tiny flaws is high and metal and PR defects are difficult to distinguish, the scheme innovatively introduces a multi-mode collaborative acquisition mechanism, and the detection accuracy is improved through millisecond-level synchronization and space registration of four sets of bright field, dark field, ultraviolet and structured light imaging systems. A comprehensive data cube containing textures, metal features, fluorescence response and three-dimensional morphology is constructed, full-dimensional capture and accurate characterization of micron-sized flaws are achieved, and the detection rate and classification accuracy of complex flaws are remarkably improved.

The invention discloses an indole-benzothiadiazole derivative as well as a preparation method and application thereof. The general structural formula of the indole-benzothiadiazole derivative provided by the invention is shown in the specification, wherein R is selected from one of methyl piperazinyl, diethylamino, azetidinyl and pyrrolidinyl. The derivative can be specifically combined and fluorescently labeled with mitochondrial RNA in cells, and has good selectivity, high fluorescence signal-to-noise ratio and excellent anti-interference capability in a complex biological system. Meanwhile, the indole-benzothiadiazole derivative provided by the invention is simple in synthetic route, easily available in raw materials, good in stability and convenient to store and use, can be specifically combined with RNA and generate strong fluorescence response, is suitable for real-time detection and imaging of in-vitro and in-vivo mitochondrial RNA, and is an efficient mitochondrial RNA micromolecular fluorescent probe.

The invention belongs to the technical field of water pollutant identification, and particularly relates to a water pollutant distinguishing method and system based on multi-source spectrum fusion. According to the method, ultraviolet visible spectrum data and three-dimensional fluorescence spectrum data are effectively integrated in a multi-source spectrum data fusion mode, so that the characteristic information of water pollutants is captured, stably appearing characteristic absorption wavebands can be positioned by utilizing the ultraviolet visible spectrum data, and the water pollutants can be accurately detected. The method comprises the following steps: identifying a characteristic sub-region capable of representing the fluorescence response characteristic of a pollutant through three-dimensional fluorescence spectrum data, in a characteristic extraction stage, performing first-order derivative operation on an ultraviolet visible spectrum to position an absorption peak position, calculating curvature distribution of a three-dimensional fluorescence spectrum to identify the characteristic sub-region, and when a fusion characteristic is generated, performing first-order derivative operation on the three-dimensional fluorescence spectrum to identify the characteristic sub-region; according to the method, the key operation parameters are subjected to grid search optimization, and the optimal key operation parameter combination is screened out by performing multiple rounds of iterative training and evaluation on a preset training set and a verification set, so that the recognition performance of the real-time collected water sample is improved.

The invention discloses a visual detection test method for damage cracks of a solidified soil test piece, and particularly relates to the technical field of geotechnical engineering material detection. The method comprises the following steps: doping fluorescence labeling particles in a solidified soil test piece, and applying periodic load to induce crack formation after curing and forming; synchronously exciting fluorescent particles through an ultraviolet excitation light source, and collecting a short-wave fluorescence response image sequence; inputting the image sequence into a deep residual neural network model, identifying a nonlinear enhancement region of a fluorescence signal, and deducing a crack initiation position and a propagation path; constructing a three-dimensional dynamic model of crack evolution in combination with an image space reconstruction algorithm and time sequence comparison, extracting indexes such as a crack propagation rate, a bending angle and crack density, performing comparative analysis on the indexes and initial parameters of a material, evaluating a microstructure damage development mechanism, and outputting a visual damage evolution result; the method provided by the invention can realize high-precision, non-destructive and dynamic detection of the fine cracks in the solidified soil, and has the advantages of high identification sensitivity, strong modeling precision and good engineering adaptability.

The invention relates to the technical field of biomedical analysis instruments, and discloses a DNA quantitative fluorometer calibration system based on artificial intelligence optimization, which comprises a data acquisition module for acquiring a dynamic fluorescence characteristic spectrum for representing the whole process of fluorescence reaction; and an intelligent calibration and diagnosis core model in the data processing unit analyzes the spectrogram by using a long short-term memory network and an attention mechanism so as to generate a multi-dimensional diagnosis result containing predicted concentration, confidence score and interference early warning. According to the method, the dynamic fluorescence characteristic spectrum of the standard substance is used as a scene anchor point, the preset global basic model is finely adjusted, and the special session model is generated, so that the real-time self-adaptive calibration of the change of the instrument and the reagent is realized. According to the method, the defects that a traditional method depends on an end point value, is easily interfered and is static in calibration are overcome, and the accuracy, reliability and long-term stability of a DNA quantitative result are remarkably improved.

The invention relates to the technical field of electrocatalytic oxidation, and provides a fluorescence detection condition control system and method, and the system comprises a strategy library which uses XGBoost to train strategy algorithm models of different water sample water qualities and initial wave bands; the initialization module is used for calling the strategy library to load the water quality type according to a detection environment and setting initial state water sample condition matching; the pre-excitation module is used for lightening the single-band LEDs one by one to obtain an initial fluorescence response value; the self-adaptive detection module is used for calling a strategy algorithm model and generating a wave band combination and excitation intensity configuration according to the initial fluorescence response value; the temperature compensation module monitors the key temperature in real time through a temperature sensor and carries out compensation correction according to a fluorescence error caused by temperature drift; and the analysis report module outputs a Cu < 2 + > concentration detection result. According to the method, the water quality preset strategy library and the temperature compensation mechanism are combined, Cu < 2 + > fluorescence detection under the complex matrix background is achieved, and the accuracy and environmental adaptability of low-concentration heavy metal detection are remarkably improved.

The invention discloses a graph pattern generation method based on a Clarii graph, and relates to the technical field of information, and the method comprises the steps: constructing an extended fluorescent material calibration module, introducing a saturation critical light intensity reference value, and carrying out the calibration of the Clarii graph under the dual constraints of color temperature deviation distribution and brightness standard deviation; according to the method, the pattern contrast change condition is recognized through the adjusted pattern contrast, a correction index is formed, the LED backlight wavelength is corrected for the second time according to the correction index, pattern structure deviation information is fed back to a spectrum control link, and the color uniformity of the LED backlight is improved. According to the scheme, on the premise of ensuring uniform fluorescence response and inhibiting bright spots, dark spots and stripes, the Clariy pattern can be kept stable, clear and layered in an actual application environment.

The application belongs to the field of explosive detection, and discloses a polyimide loaded triaminophthalocyanine probe, a preparation method thereof and application thereof in detecting nitroaromatic explosives. The probe has the following general structure formula. The probe is prepared by cross-linking copolymerization of 5,10,15-tri(4-aminophenyl)-20-phenyl porphyrin, aromatic tetracid dianhydride and aromatic organic diamine. The polyimide high-molecular cross-linked branch loaded triaminophthalocyanine compound with fluorescent light emitting performance is prepared for the first time. The probe is applied to detecting various nitroaromatic explosives by quenching fluorescence response, and the detection method is simple, and has good application prospect in the field of public security.

The invention discloses a multi-emission composite nano ratiometric fluorescent probe for detecting fluoroquinolone antibiotics and a preparation method of the multi-emission composite nano ratiometric fluorescent probe. The CdTe-Tb-UMP composite nano ratiometric fluorescent probe with double ligands is prepared by taking metal terbium ions (Tb < 3 + >) as central ions and respectively taking uridine monophosphate (UMP) and CdTe quantum dots with red fluorescence emission as bridged ligands. ENR is further coordinated with Tb < 3 + >, energy is transferred to Tb < 3 + > through an antenna effect, so that characteristic green fluorescence of Tb < 3 + > is turned on, and fluorescence of CdTe quantum dots is almost kept unchanged. On the basis, a portable nursing point testing system is further constructed, and the portable nursing point testing system comprises an intelligent mobile phone detector and a wearable fingertip sensor integrated with a micro-fluidic chip and is used for on-site rapid detection of the ENR. The paper-based chip shows rapid and sensitive fluorescence response, and the detection limits of the spectrograph and the paper-based sensor on the ENR are 4.7 nM and 13.1 nM respectively. The probe can be applied to detection of ENR in actual environment water samples, food and drugs.

The application discloses a three-channel fluorescence sensor array based on sulfur quantum dots and a preparation method and detection application thereof. The sensor array is composed of three kinds of sulfur quantum dots, namely C-SQDs, PSS-SQDs and beta-SQDs. When target objects exist, the three kinds of sensing units generate differentiated fluorescence responses, forming a unique "fingerprint map". By using principal component analysis to analyze the response mode, the array can successfully distinguish between structurally similar baicalein, fiscetin, myricetin, quercetin and rutin in a concentration range of 8.0-56 muM. In addition, the array can also perform semi-quantitative analysis on a single flavone compound in a range of 4.0-56 muM, and the first principal component has a good linear relationship with the logarithm of the concentration of the target object.

The invention discloses a multi-parameter wearable intelligent optical fiber sensing system, and the system comprises a fluorescent sensing patch which is used for detecting a target object through a fluorescent reaction, and comprises a biosensor which is used for reacting with the target object and generating a fluorescent signal; the data glove is used for monitoring the bending state of finger joints in real time and achieving gesture recognition and comprises a glove body and a plurality of optical fiber bending sensors attached to the joint portions of the glove body, and the optical fiber bending sensors reflect the finger bending degree in real time through light intensity changes and output multi-dimensional light intensity signals. And the wearable optical fiber detector is connected with the fluorescent sensing patch and the data glove and is used for collecting and analyzing the fluorescent signal and the bending signal. The multi-parameter wearable optical fiber intelligent sensing system has multi-channel and full-automatic intelligent detection and analysis capability, can provide real-time monitoring and analysis services of multiple physical and chemical parameters such as fluorescence, gesture bending and environment temperature and humidity at the same time, and provides powerful technical support for application in various fields.

This application discloses an embedded whole-cell biosensor, its fabrication method, and its application in machine learning-assisted mercury ion detection, belonging to the field of sensor materials and detection technology. This sensor encapsulates *E. coli* expressing green fluorescent protein (GFP) in a polyacrylamide / hyaluronic acid (PAAM / HA) hydrogel matrix, enabling highly selective and sensitive detection of mercury ions. It effectively eliminates interference from competing metal ions and can be completely regenerated through EDTA-2Na treatment, allowing for repeated use without performance loss. It maintains a stable fluorescence response for up to three weeks, possesses excellent biocompatibility and biodegradability, minimizing the risk of secondary contamination, and facilitates rapid on-site analysis.

The present disclosure relates to a diagnostic cartridge for immunodiagnosis, and a reader and a diagnostic system using the same. A diagnostic cartridge, including the diagnostic pad and the color reaction pad, is provided to detect a color reaction and a fluorescence reaction through the single diagnostic cartridge.

This invention discloses a eutectic material with ion-stimulated fluorescence response and its preparation method. The eutectic material is a co-crystallized compound formed by a fluorescent cyclophenone and a crown ether through a host-guest interaction. The eutectic material and its preparation method provided by this invention utilize a fluorescent cyclophenone as the host and a crown ether as the guest, and the two are combined through a host-guest interaction to obtain the eutectic material. This material not only possesses ion-stimulated fluorescence response characteristics but also has advantages such as high spatiotemporal resolution and good sensitivity.

This utility model relates to the field of microbial detection technology, and more specifically, to a temperature-controlled handheld ATP fluorescence detector. It includes a housing, a temperature control component, and a main circuit board and a detection mechanism respectively disposed within the housing. The detection mechanism has a detection chamber for holding a swab. The main circuit board integrates a central processing module and a fluorescence detection module. The temperature control component includes a heating element and a temperature sensor respectively installed in the detection mechanism. The signal output terminal of the temperature sensor is electrically connected to the central processing module, the signal output terminal of the central processing module is electrically connected to the heating element, and the signal output terminal of the fluorescence detection module is electrically connected to the central processing module. The temperature sensor senses the temperature of the reagent. If the detected reagent temperature is lower than the optimal temperature range for the ATP fluorescence reaction, the heating element will activate to heat the reagent to the optimal temperature range. This ensures detection accuracy even in low-temperature environments and provides high environmental adaptability.

This invention relates to a method for preparing and applying an acidic medium-based iron ion recognition indicator. The indicator contains an aminoquinoline structure and a sulfosalicylic acid structure. It exhibits excellent dual colorimetric and fluorescence responses to ferric ions. When the sample contains Fe... 3+ At that time, Fe 3+ It can undergo complexation reactions with the -NH-CO-, -OH, and quinoline-N groups in the indicator, changing the solution from colorless to brown. This allows for qualitative analysis of iron ions by naked-eye observation. Simultaneously, the fluorescence weakens, and the fluorescence intensity is similar to that of Fe. 3+ The content exhibits a good linear relationship, reaching the nanomolar level, enabling quantitative detection of iron ions and Fe. 3+ It has extremely excellent selectivity, and can identify and detect iron ions not only in normal environments, but also accurately identify iron ions under extreme conditions of strong acidity. It is stable, can be used in acidic media, and has strong anti-interference ability.

The invention discloses a series of photoinduced isocompounds based on an alpha-cyano diarylethene skeleton as well as a preparation method and a metal ion regulation mechanism of the photoinduced isocompounds. The photoinduced isomeric compound is prepared through Knoevenagel condensation reaction by taking alpha-cyano diarylethene as a basic skeleton structure, the structural general formula of the photoinduced isomeric compound is shown in the specification, and the photoinduced isomeric compound is named as a hydroxyl-containing alpha-cyano diarylethene aromatic derivative. The photoinduced isomerization compound disclosed by the invention has good polarity sensitivity and photoinduced isomerization capability, and Z / E isomerization can be realized under the irradiation of ultraviolet light. The addition of metal ions is not a fluorescent response trigger factor, but can effectively adjust the photoresponse degree and rate of the photoinduced isocompound. The synthesis method of the photoisomerization compound is simple, the product is easy to purify, the optical property caused by photoisomerization can be regulated and controlled through coordination of polarity and metal ions, and an effective strategy is provided for designing a high-performance optical function material and application thereof.

The invention discloses a kit for identifying a G2b gene subtype of a porcine epidemic diarrhea virus and application of the kit, and belongs to the field of virus nucleic acid detection. Through gene comparison, primers and probes are designed at specific sequence positions of the PEDV G2b gene subtype, and quantitative PCR identification detection can be directly carried out through an LNA technology. The detection method only comprises two steps of nucleic acid extraction and one-step fluorescent RT-PCR reaction, can complete identification within 2 hours at the soonest, has the characteristics of good specificity, high sensitivity and good stability, can rapidly, simply, conveniently and efficiently identify and detect PEDV G2b subtype nucleic acid, is low in cost and short in period, is especially suitable for high-throughput detection, and can be widely applied to detection of PEDV G2b subtype nucleic acid. And the method has important significance on rapid identification of PEDV G2b gene subtype strains.

The invention belongs to the technical field of coal mine safety monitoring, and discloses a goaf coal spontaneous combustion hidden fire source fluorescent nanoparticle dynamic real-time temperature sensing detection method, which is based on the temperature-sensitive fluorescence characteristic of fluorescent nanoparticles, and comprises the following steps of: distributing a detection medium loaded with the fluorescent nanoparticles to a goaf; a fluorescence signal acquisition device is used for receiving fluorescence response signals generated by fluorescent nanoparticles under the influence of temperature in real time, and a signal analysis system is combined for processing and analyzing characteristic parameters of the fluorescence signals, so that dynamic real-time detection of the position, the temperature and the development trend of a coal spontaneous combustion hidden fire source in a goaf is realized.

The invention relates to a preparation method and application of a sprayable surface-adaptive packaging film for in-situ detection of organophosphorus pesticides, according to the method, carboxylated cellulose nanofiber mediated defect type UiO-66-OH is used as a core functional material, sprayable slurry is prepared in cooperation with an aqueous film-forming matrix, and a continuous compact film layer is formed through a synchronous spraying process. The method is simple in processing, manufacturing tools are easy to obtain, and batch production and preparation can be realized; the packaging film obtained through the method can provide a stable and independent reaction and fluorescence response environment for in-situ detection and preliminary degradation of organophosphorus pesticides, the detection-degradation-protection integrated function is achieved, the problems that in-situ fitting performance is poor, the function is single, operation is tedious, and pollution is prone to occurring in the prior art are solved, and the packaging film has good application prospects. The paper achieves multiple functions, is suitable for detecting organophosphorus pesticide residues on the surfaces of various fruits and vegetables, and is environment-friendly, high in stability, easy to store and low in cost.

The invention discloses a method for quantitatively detecting uranyl ions by adopting a silicon-based carbon dot material and application of the method, and belongs to the field of nano composite materials and radionuclide detection. Mixing the silicon-based carbon dot composite material with an acid solution containing uranyl ions, carrying out ultrasonic treatment, detecting the fluorescence intensity of the mixed solution by adopting a fluorescence spectrum, and quantitatively analyzing the uranyl ion concentration according to the uranyl ion concentration and a fluorescence intensity curve. The silicon-based carbon dot composite material is a silicon-based carbon dot material of which the surface is modified by functional groups; the functional groups comprise hydroxyl, carboxyl and amino. The method can generate stable fluorescence response change when acting with uranyl ions, accurate quantitative analysis of uranyl ion concentration can be realized, the silicon-based carbon dot composite material has high uranyl ion detection response speed by means of the synergistic effect of the nanoscale effect and the surface functional group, and detection can be completed in a short time.

The utility model discloses a leakproofness detection device for a transformer insulating oil delivery pipeline, which comprises a pressure detection unit and an oil delivery pipeline used for installing the pressure detection unit, one end of the oil delivery pipeline is provided with a fluorescent agent injection port, and the top of the oil delivery pipeline is fixedly communicated with two groups of connecting pipes. The top ends of the two sets of connecting pipes are fixedly communicated with a U-shaped pipe, the pressure detection unit is communicated with the U-shaped pipe, the top of the oil conveying pipeline is communicated with the U-shaped pipe, a positioning detection assembly is integrated, rapid and accurate positioning of the leakage position is achieved, and when leakage happens to the oil conveying pipeline, a fluorescent agent seeps to the leakage point along with insulating oil; the ultraviolet lamp moves and irradiates along the oil conveying pipeline, the leakage position can be visually located by detecting the fluorescence reaction, the problem that in the prior art, detection only depends on the pressure attenuation rate, but the leakage point cannot be rapidly located is solved, and the detection efficiency and accuracy are remarkably improved.

The invention provides a specific probe for detecting perfluorinated compounds in a water body and a detection method thereof, and belongs to the technical field of detection. The probe provided by the invention is a compound formed by cucurbituril and fluorescein; the detection method provided by the invention comprises the following steps: adding a cucurbituril and fluorescein conjugate into a to-be-detected water sample containing the perfluorinated compounds, carrying out equilibrium reaction for a period of time, and then detecting the fluorescence response intensity in the water body so as to quantitatively evaluate the concentration of the specific perfluorinated compounds in the water sample. Compared with the prior art, the probe equipment provided by the invention is small in size, convenient to carry, capable of simultaneously detecting multiple samples and short in reaction time; the whole material of the probe is green and harmless, and chemical agents such as sulfate which easily cause secondary pollution are not used; other impurity removal and enrichment small columns and complex masking agents do not need to be used. In addition, the detection method provided by the invention has a lower detection limit, the sensitivity and convenience of novel perfluorinated compound detection are remarkably improved, and the application range is wider.

This invention discloses an electrochemical-fluorescence dual-mode biosensor for the detection of bladder cancer-related miRNA biomarkers and its application. The sensor is a homogeneous biosensor comprising a CRISPR / Cas13a system for specific recognition of target miRNAs, magnetic beads, multiple nucleic acid substrates, and one or more signal probes. The CRISPR / Cas13a system includes the Cas13a protein and crRNA with a complementary sequence to the miRNA. The magnetic beads bind to nucleic acid substrate 1, serving as the reaction substrate. After activation by the target miRNA, the CRISPR / Cas13a system trans-cleaves the ribonucleotide sites in the nucleic acid substrate 1 sequence bound to the magnetic beads. Magnetic separation of the magnetic beads and supernatant forms a dual-sensing circuit. The dual-sensing circuit reacts with different substrate nucleic acids and signal probes respectively, outputting electrochemical and fluorescence response signals, achieving single-shot dual-mode detection of the target miRNA.

The invention provides a fluorescent anti-forgery ink luminescence effect characterization and quality control method, which comprises the following steps: adding a fluorescence correction agent into a solvent for constant volume, and preparing a standard solution; coating the printing ink on the surface of paper or dispersing the printing ink into a solvent to prepare a color developing sample or a solvent dispersing sample; respectively detecting and analyzing the ink sample and the fluorescence correction agent standard solution by adopting a fluorescence spectrophotometer, and correcting the fluorescence spectrum peak of the ink sample based on the response of the fluorescence correction agent to obtain a sample correction spectrogram; and constructing a quality control interval of the fluorescence response of the ink sample by taking the fluorescence response in the correction spectrogram of the series of ink samples as a data point, and evaluating the quality of a subsequent to-be-detected sample. The invention provides a sample preparation, correction and quality control method of a fluorescent anti-counterfeiting ink luminescence effect characterization system for the first time, provides a quantitative evaluation mode for fluorescent anti-counterfeiting, and solves the problems of poor visual observation accuracy and large subjective influence.

A method for rapidly detecting and identifying food-borne pathogens by using a two-dimensional / zero-dimensional MXene / GQDs Schottky heterojunction fluorescence sensor array based on machine learning comprises the following steps: synthesizing three nitrogen-doped graphene quantum dots through a hydrothermal method, determining the optimal excitation wavelength, concentration, reaction temperature and pH value of the nitrogen-doped graphene quantum dots, and further determining the fluorescence intensity of the nitrogen-doped graphene quantum dots under the optimal condition; constructing a fluorescence sensor array, mixing the three graphene quantum dots with target heavy metal ions, incubating, then measuring fluorescence intensity to obtain fluorescence response data, and normalizing the data to obtain the fluorescence response data. And analyzing by using machine learning methods of a decision tree, a support vector machine, K nearest neighbor, linear discriminant analysis, naive Bayes and a neural network. The prepared fluorescence sensor array can rapidly identify heavy metal ions, is excellent in accuracy, sensitivity and stability, provides an economical and efficient solution for heavy metal ion detection, and has remarkable application potential in environmental monitoring.

The application relates to the field of optical measurement, and discloses a micropore detection assembly and method. The assembly comprises a light source for emitting excitation light, which is used for irradiating a micropore of an organic plate material, so that the organic material on the side wall and the bottom of the micropore generates a fluorescent reaction and emits fluorescence; a dichroic mirror which is arranged opposite to the light source and is used for reflecting the excitation light so that the excitation light propagates along the optical axis of a light converging element and is transmitted; a light converging element which is arranged on the side of the dichroic mirror close to the organic plate material and is used for converging the excitation light reflected by the dichroic mirror; the converged excitation light is vertically incident to the micropore; and an image sensor which is arranged on the side of the dichroic mirror far away from the organic plate material and is used for receiving the fluorescence transmitted through the dichroic mirror and generating an image including the micropore. The excitation light is used for making the inside of the micropore emit fluorescence, so that the image definition is improved, the images of the top and the bottom of the micropore are clear, and the detection efficiency is improved. In addition, residues in the micropore can also be identified.

To improve fluorescence luminance stability for each measurement by performing fluorescence measurement by charging an observation liquid whose temperature is controlled.SOLUTION: A reaction measuring apparatus comprising: a reaction chamber for supplying a sample liquid and a reagent liquid containing a fluorescent label to an array plate to produce a reaction product of a substance contained in the sample liquid, the fluorescent label, and a substance immobilized as a spot on the array plate; and a measuring chamber for irradiating the spot with excitation light to measure fluorescence emitted from the reaction product. The reaction chamber includes a reaction chamber mounting section on which an array plate is mounted, a sample liquid mounting section, a reagent liquid mounting section, a buffer liquid mounting section, an observation liquid mounting section, and a liquid supply section for supplying a sample liquid, a reagent liquid, a buffer liquid, and an observation liquid to the array plate. The observation liquid placement section is thermally coupled to the chamber atmosphere of the measurement chamber or the measurement chamber placement section.SELECTED DRAWING: Figure 1

The invention provides a flexible light-emitting film and a preparation method and application thereof, and belongs to the technical field of light-emitting materials. According to the light-emitting film, PDMS is used as a substrate, transparent (ECMP) 2MnBr4 crystals are used as a light-emitting material, and the light-emitting film has good flexibility and visibility. The (ECMP) 2MnBr4 crystal triggers mechanoluminescence through hydrogen bond fracture and defect auxiliary charge transfer under mechanical stress, so that the mechanoluminescence performance of the luminescent film is stable and recoverable. In the tensile range of 20-100%, the mechanoluminescence (ML) intensity is linearly increased along with tensile strain, and the luminescent property is sensitive. The flexible light-emitting film prepared by the invention is green and simple in preparation process, the raw materials are non-toxic, and the flexible light-emitting material is quick in fluorescence response to mechanical stimulation and high in sensitivity, and has a good application prospect in the field of disaster early warning (collapse and crack propagation).

The invention belongs to the technical field of fluorescent probes, and particularly relates to a preparation method and property research of a dual-response fluorescent probe for detecting polarity and peroxynitrite. The preparation method of the fluorescent probe is simple, the product yield is high, and the fluorescent probe is suitable for large-scale popularization and application. The present invention relates to a method for preparing a probe (Z)-4-(1-cyano-2-(6-(4-(diphenylamino) phenyl)-4-phenyl-4H-dithieno [3, 2-b: 2 ', 3'-d] pyrrol-2-yl) vinyl)-1-(4-(4, 4, 5, 5-tetramethyl-1, 3, 2-dioxaborolan-2-yl) benzyl) pyridine-1-onium bromide (DTBP). When excitation is carried out at 600 nanometers, the fluorescence intensity is gradually enhanced along with the reduction of the polarity of the solution; in a solvent of PBS and acetone in equal proportion, when excitation is carried out at 470 nanometers, the probe reacts with ONOO to generate strong fluorescence. Along with the increase of the ONOO concentration, the fluorescence intensity is gradually enhanced. The probe can specifically detect ONOO, and has a good linear relationship. The probe also has relatively strong fluorescence response to polarity, and the fluorescence emission intensity is gradually increased along with the reduction of the polarity.