Silicon-nanowire-based alkaline phosphatase fluorescent chemosensor, preparation method and application

A technology of chemical sensor and silicon nanowire, applied in the field of fluorescent chemical sensor, can solve the problem of unclear alkaline phosphatase

Active Publication Date: 2014-07-23
TECHNICAL INST OF PHYSICS & CHEMISTRY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, how alkaline phosphatase plays a regulatory role at the single-cell level in pathological processes is still unclear.

Method used

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  • Silicon-nanowire-based alkaline phosphatase fluorescent chemosensor, preparation method and application
  • Silicon-nanowire-based alkaline phosphatase fluorescent chemosensor, preparation method and application
  • Silicon-nanowire-based alkaline phosphatase fluorescent chemosensor, preparation method and application

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Experimental program
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Effect test

Embodiment 1

[0035] 1) At room temperature, put silicon monoxide into a porcelain boat after being ground by a mortar, and place the porcelain boat in the middle of the quartz tube of a horizontal tube furnace, and then vacuumize the system to 10 with a mechanical pump and a molecular pump. -3 Pa, then feed the mixed gas of argon (accounting for 95% of the volume of the mixed gas) and hydrogen (accounting for 5% of the volume of the mixed gas) at a flow rate of 50 sccm (mL / min), when the pressure is stabilized at 1000Pa, the system starts to heat up ;The system raised the temperature to 300°C at 10°C / min, and then raised to 800°C at 20°C / min. At this time, close the air valve and pump gate, keep it for 10 minutes, then continue to raise the temperature to 1350°C, and keep the temperature for 7 hours. The temperature of the furnace is naturally lowered; the product silicon nanowires are collected on both sides of the porcelain boat. The obtained silicon nanowires are single crystal silicon w...

Embodiment 2

[0042] 1) Take n(100) silicon wafers of 2cm×1.0cm, and ultrasonically clean them with acetone, ethanol, and distilled water for 10 minutes; take out the cleaned silicon wafers and place them in AgNO with a concentration of 5mmol / L. 3 and 4.8mol / L HF mixed aqueous solution; after soaking for 5 minutes, take it out and put it into 10mL containing 4.8mol / L HF and 0.2mol / L H 2 o 2 In the mixed aqueous solution, the system was kept warm in a water bath at 50°C; after 15 minutes, the silicon chip was taken out and placed in a mixed solution containing 4.5mL concentrated hydrochloric acid (36% in mass concentration) and 1.5mL concentrated nitric acid (36% in mass concentration). Take out the silicon wafer after soaking for 0.5 hour, wash it with distilled water, and place it in a watch glass to dry naturally after rinsing to obtain a silicon nanowire array made of silicon nanowires, wherein the diameter of the silicon nanowires in the silicon nanowire array is 200-400nm, length 15-2...

Embodiment 3

[0050] 1) At room temperature, put silicon monoxide into a porcelain boat after being ground by a mortar, and place the porcelain boat in the middle of the quartz tube of a horizontal tube furnace, and then vacuumize the system to 10 with a mechanical pump and a molecular pump. -3 Pa, then feed the mixed gas of argon (accounting for 95% of the volume of the mixed gas) and hydrogen (accounting for 5% of the volume of the mixed gas) at a flow rate of 20 sccm (mL / min), when the pressure was stabilized at 700Pa, the system began to heat up ;The system raised the temperature to 300°C at 20°C / min, and then raised to 800°C at 10°C / min. At this time, the air valve and pump gate were closed, and the temperature continued to rise to 1350°C after keeping for 30 minutes, and the temperature was kept for 3 hours. The temperature of the furnace is naturally lowered; the product silicon nanowires are collected on both sides of the porcelain boat, and the obtained silicon nanowires are single ...

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Abstract

The invention belongs to the field of nanostructured fluorescent chemosensors, and especially relates to a silicon-nanowire-based alkaline phosphatase fluorescent chemosensor and a preparation method, and application of the fluorescent chemosensor. The silicon-nanowire-based alkaline phosphatase fluorescent chemosensor is a silicon-nanowire or silicon-nanowire-array fluorescent chemosensor with the surface modified by phosphorylated fluorescein molecules possessing selective fluorescence response to alkaline phosphatase. The silicon-nanowire-based alkaline phosphatase fluorescent chemosensor is applicable to detect alkaline phosphatase molecules in a solution system containing alkaline phosphatase molecules. A single-silicon-nanowire fluorescent chemosensor scraped off from a silicon-nanowire array has wide application prospect on detection of alkaline phosphatase molecules in unicell level, and helps to provide a new sensor for directly detecting the activity of alkaline phosphatase in cells.

Description

technical field [0001] The invention belongs to the field of fluorescent chemical sensors with nanostructures, in particular to an alkaline phosphatase fluorescent chemical sensor based on silicon nanowires, a preparation method thereof, and an application of the fluorescent chemical sensor. Background technique [0002] Alkaline phosphatase is a kind of phosphohydrolase, which is widely distributed in various organs of the human body, especially in the liver, bones and kidneys. The abnormal content of alkaline phosphatase in serum is related to bone disease, abnormal liver function, diabetes and many other diseases. At present, there are many techniques for the detection of alkaline phosphatase, including fluorescence, colorimetry, electrochemical methods, and surface-enhanced Raman scattering. Among them, fluorescence technology has shown great advantages in the detection of intracellular and extracellular alkaline phosphatase because of its high sensitivity and high spat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/06C09K11/59B82Y30/00B82Y20/00G01N21/64C12Q1/42
Inventor 穆丽璇王会敏师文生
Owner TECHNICAL INST OF PHYSICS & CHEMISTRY - CHINESE ACAD OF SCI
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