83results about How to "Meet the requirements of rapid detection" patented technology

The invention discloses a primer and probe for detecting a human epidermal growth factor receptor (EGFR) gene and / or a K-ras gene, a kit containing the primer and the probe and a device for detecting genetic mutation on the basis of a digital PCR platform.The method for detecting the genetic mutation by means of the primer and the probe comprises the steps that the prime and the probe are provided; DNA of a sample to be detected is extracted; a fluorescent PCR reaction system capable of amplifying a mutant gene sequence is prepared; a target probe and an internal reference probe are utilized to be hybridized with amplified products respectively, and fluorescent signals of corresponding fluorescent groups are detected; existence of the genetic mutation is judged and / or the mutation rate is calculated according to the strength and proportion of the fluorescent signals of the target probe and the internal reference probe.According to the method for detecting the genetic mutation, the needed primers and probes are small in number, the optimization procedure is simple, related mutation of EGFR and / or K-ras gene can be qualitatively or quantitatively detected, and the detection sensitivity is high; a DNA sample with low initial amount can also be detected stably.

The invention discloses a clenbuterol hydrochloride assay kit and its preparation method and use method. The clenbuterol hydrochloride assay kit comprises a kit body, a clenbuterol hydrochloride enzyme label plate, a clenbuterol hydrochloride standard solution, a clenbuterol hydrochloride enzyme conjugate, a diluent, a concentrated washing liquid, a first substrate and a second substrate. The preparation method provided by the invention comprises preparing the clenbuterol hydrochloride enzyme label plate, the clenbuterol hydrochloride standard solution, the clenbuterol hydrochloride enzyme conjugate, the diluent, the concentrated washing liquid, the first substrate and the second substrate. The use method provided by the invention comprises sample preparation, sample addition and detection, and detection result analysis. The clenbuterol hydrochloride assay kit has the advantages that sensitive, stable and reliable result analysis is realized; operation steps are less; sample pre-treatment is simple; adopted equipment can be popularized easily; and the clenbuterol hydrochloride assay kit can be utilized for determination of residual clenbuterol hydrochloride of pork, has sensitivity of 0.03 micrograms per kilogram, needs detection time shorter than detection time of an ELISA method by 30 minutes and satisfies requirements of rapid detection of clenbuterol hydrochloride residues.

The present invention relates to a method for detecting acrylamide in food, and particularly to a quick fluorescent detecting method for detecting AM content in toasted, fried, or baked foods, which is characterized in easy operation, high sensitivity and repeatability, quick and accurate detection, high application value, high economical efficiency and high reliability, and easy application. The method comprises: loading pulverized sample into a container, adding normal hexane, performing ultrasonic degreasing, and blowing dry the residual normal hexane to obtain a product A; adding NaCl solution into the product A, performing ultrasonic extraction and centrifugation, and then re-extracting from the residue with NaCl solution, and combining the liquid extract; adding NaOH solution and then sodium hypochlorite solution into the liquid extract, conditioning the mixed solution after reaction with a phosphate buffer solution to alkaline, and finally adding fluorescamine solution and mixing, and loading the mixed solution to a luminoscope to test.

The invention relates to a full coupled test probe for dry hole sound wave, and belongs to the field of nondestructive examination equipment manufacture of engineering. The full coupled test probe for the dry hole sound wave comprises a sound wave probe and a pressurizing water bag, wherein the top at one sealed end of the pressurizing water bag is provided with a vent hole of which the diameter is not more than 1 millimeter. An end part of the sound wave probe is provided with a connecting device; and the pressurizing water bag is sleeved outside the sound wave probe. Air in the pressurizing water bag can be directed exhausted by the vent hole; and the pressurizing water bag is filled with water at the same time to ensure internal coupling between the sound wave probe and the pressurizing water bag. The vent hole at the top of the pressurizing water bag has double uses, namely, after the air is exhausted, the vent hole continuously keeps continuous small amount of spilling water, so as to solve coupling (external coupling) between the outer wall of the pressurizing water bag and the wall of the hole. The test probe realizes full coupling of the sound wave prove, the pressurizing water bag and the wall of the hole so that the tested data are stable and reliable, and meets the requirement of rapid detection.

The invention discloses a portable electrochemical detection and analysis device, which comprises a single-chip microcomputer module, a program storage module, a data acquisition module, an output module, a temperature compensation module and a power supply module. The invention adopts components with high integration and low power consumption. The device is small in size and portable. It is equipped with a single-chip microcomputer and a liquid crystal display, which can analyze and display the detection results and meet the requirements of rapid detection. It is suitable for medical detection, food safety, etc. It can be used on-site for analysis in fields such as environmental protection and environmental protection. It is powered by a battery and a USB interface. When there is no external power input in the field, it can be powered by a battery or a battery in a notebook PC to power the device; it has a data memory and can store history. Data, with a USB data transmission interface, can be connected to peripherals such as a host computer and a printer, and can print detection values ​​and curves, etc., and can perform various analyzes on the stored data through the host computer to obtain detailed information about the research system.

The invention discloses an electrochemical sensor for quickly detecting Sudan I in foods and a preparation method thereof. The preparation method of the sensor comprises the following steps of: uniformly mixing montmorillonite, conductive carbon powder and adhesive at a certain percentage to form a sensitive material; filling the sensitive material in a cavity on the front end of an insulating column and enabling the sensitive material contact with the end of a metal conductor inserted and fixed at the centre of the insulating column. The electrochemical sensor of the invention has the advantages of high sensitivity, low detection limitation, capability of realizing quick detection to the Sudan I in foods, simple and convenient operation, environment-friendliness, wide raw material sources and low cost; moreover, the preparation method is simple.

The invention relates to the technical field of ore detection, in particular to a method for measuring the contents of carbon and sulfur in chromium ores. The method comprises the following steps: S1,smashing a sample to be measured till the sample can pass through sieve pores of 100 meshes, and drying the sample; S2, weighing 0.05 to 0.5 weight part of the sample to be measured, putting the sample to be measured into a crucible which is burnt, and is laid with 0.38 to 0.42 weight part of a pure iron fluxing agent at the bottom, covering the surface of the sample to be measured with 1.1 to 1.3 weight parts of tungsten particles and 0.24 to 0.26 weight part of tin particles, and detecting the sample through a high-frequency infrared carbon-sulfur instrument, thereby obtaining the contentsof the carbon and the sulfur. The method for measuring the contents of the carbon and the sulfur in the chromium ores is easy to operate, high in detection speed and low in cost; the detection range is 0.1 to 15 percent; the accuracy and the precision can meet requirements for measurement of the contents of carbon and sulfur in imported and exported chromium ores; the method has the advantages ofshort detection period, accurate result and saving of reagent and personnel investment, can completely meet a requirement of the foreign trade for inspection, quarantine and quick detection, is relatively high in practicability, and can be widely popularized and applied.

The invention discloses a method for measuring content of oxygen, nitrogen and hydrogen in a titanium alloy. The method comprises the following steps: (1) performing treatment such as cutting and polishing on a high-purity electrolysis nickel board, thereby preparing a nickel particle fluxing agent; (2) putting the nickel particle fluxing agent into an inner high-purity graphite crucible of a lower electrode of an oxygen, nitrogen and hydrogen analyzer, and performing degassing treatment under high power; (3) selecting a measuring condition, and measuring oxygen, nitrogen and hydrogen gas in the titanium alloy through the oxygen, nitrogen and hydrogen analyzer; and (4) calculating mass percentage of oxygen, nitrogen and hydrogen in a test sample. According to the method disclosed by the invention, a self-made nickel particle fluxing agent with a relatively high blank value can be used to replace commercially available fluxing agents such as a nickel basket and nickel scraps, content of oxygen, nitrogen and hydrogen in the titanium alloy is measured through a pulse melting method, interference of the blank value can be remarkably reduced or eliminated by changing normal test steps and analysis conditions, and the same effects of the commercially available fluxing agents are achieved, so that detection cost is greatly reduced; and the method has the advantages of being simple, high in accuracy and the like, and meets the quick detecting requirements.

The invention discloses a method for detecting holdup of split phases in oil sludge based on an induction heating technique and a measuring system. The detection method is based on the formation mechanism and physical-chemical characteristics of the oil sludge, the induction heating technique is adopted, the oil sludge is pyrolyzed according to the set heating curve, and the oil gas and water steam produced by pyrolysis are condensed and collected, and are sent into a metering cylinder; the oil and water in the metering cylinder are naturally separated, the volume of water at the lower layer is read via the scales of the metering cylinder, and the mass of the water is obtained by combining with density; the total mass of oil and water is weighed by an analytical balance, the mass of the water is deducted, and the mass of the oil is obtained; the mass of the oil and the mass of the water are deducted from the mass of the oil sludge, so as to obtain the mass of residue, thereby calculating the holdup of split phases in the oil sludge; by adopting the detection method, the holdup of split phases in the oil sludge can be simultaneously detected, the stored and transported oil sludge can be quickly detected within about one hour, and the detection absolute errors of the three phases are respectively smaller than 2%. The measuring system mainly consists of an induction heating powersupply, an induction heater, a temperature detection module, a controller, a condensing device and a metering test tube.

The invention relates to a method and device for detecting the complete-circle three-dimensional surface morphology of a diamond fretsaw, and the method and device can obtain the complete-circle surface morphology of the diamond fretsaw through a single measurement operation without splicing. An image data processing method is relatively simple, and the obtained surface information of the diamondfretsaw is complete. A sequence projection contour image is used for reconstructing a diamond three-dimensional morphology, thereby solving a problem that the quality of the collected image data is liable to be affected by the light reflection intensity of the surface of an object. The generated redundant data can be used for quickly achieving the analysis of two-dimensional images (including depth) of different positions of the surface of the diamond fretsaw, meets the actual requirements of the diamond fretsaw for the quick detection, and also can be efficiently used. The device has the functions of quickly obtaining the two-dimensional and complete-circle three-dimensional surface morphology of the diamond fretsaw and performing composite detection, and can meet the different detectionrequirements. Through the cooperation of a laser source and a second camera, the method and device enable the diamond fretsaw images to be convenient to process, and improve the measurement precision.Moreover, the structure is simple and the idea is ingenious.

The invention discloses an on-step type label-free rapid fluorescence mercury ion detection method. On the basis that ssDNA (Single-Stranded Deoxyribonucleic Acid) can form a stable T-Hg<2+>-T compound with Hg<2+> and can be folded into a stable barrette structure double-chain DNA (dsDNA), a fluorescent dye SG I can be embedded into a small-groove structure area of a double-spiral structure of the dsDNA, relatively intense fluorescence can be stimulated, the fluorescence intensity is in positive correlation with concentration of Hg<2+>, and rapid detection on the Hg<2+> is achieved. The detection method disclosed by the invention has a lowest detection limit of 3nM which is less than the standard of the Environmental Protection Agency for the Hg<2+> in a water sample, is good in specificity, free of metal ion interference, good in repeatability, relatively low in detection cost, simple to operate and easy to popularize and apply to on-site Hg<2+> detection with a novel detection platform, can realize automation, and has great potential in emergency treatment on heavy metals, environment monitoring, risk evaluation and management and the like.

The present invention discloses a rapid detection method for fertilizer nutrients. According to the method, 1-5 mL of concentrated sulfuric acid is added during the sample digesting process, 0-2 mL of perchloric acid (or hydrogen peroxide) is added as a digestion agent, and the resulting digested solution requiring detection can be used for colorimetric determinations or turbidimetric determinations of nitrogen, phosphorus and potassium. The method of the present invention has advantages of wide application range, stable reagent, low cost, easy availability, short digestion time, complete digestion, convenient operation, high accuracy, time saving, labor saving, and the like. With the method of the present invention, the nitrogen content, the phosphorus content and the potassium content in the fertilizer can be rapidly and efficiently detected, such that the rapid detection requirements of the fertilizer sample during the fertilizer production process and the fertilizer sale process are met, and the purposes of time saving, labor saving, convenience, rapidness, accuracy and reliability are achieved.

The invention provides a method for detecting plesimonas shigelloides. The method comprises the following steps of: adopting a loop-mediated isothermal amplification technology; using a hug A gene as a target gene; putting designed three pairs of DNA (Deoxyribonucleic Acid) primers and a DNA template to be detected to an amplification buffer solution including MgSO4 and glycine betaine; amplifying in a constant-temperature environment of 65 DEG C, and terminating the reaction in a constant-temperature environment of 80 DEG C; and detecting the amplified product. The method disclosed by the invention is simple and easy to operate, and has good specificity, sensitivity and repeatability.

The invention discloses a one-step chemiluminescence / enzyme immunoassay method and kit for detecting residue of malachite green. The method combines indirect enzyme immunoreaction and chemiluminescence technologies, simplifies the traditional two-step chemiluminescence / enzyme immunoassay method into a one-step method without additional preparation of an enzyme-labeled monoclonal antibody, and uses 15-35% acetonitrile as a sample dissolving solution based on the characteristic that the 15-35% acetonitrile has limited dissolving power of high-concentration colorless malachite green. The method is used for detecting residue of malachite green in animal-derived foods, has the characteristics of quickness, convenience, specificity, sensitivity, accuracy, wide detection range and the like, better meets the requirements of quick detection, and has favorable application prospects. The IC50 of the kit is 0.45 mu g / L, the recovery rate is 86.37-116.84%, and the accordance rate with a standard detection method is 100%, thus ensuring that the kit is suitable for trace analysis and batch detection of malachite green.

A method for preparing test paper of sulfamethazine colloidal gold detection technique anchors sulfamethazine coupled antigen and resisting mouse IgG antibody as well as sulfamethazine single resistance being labeled with colloidal gold on nitrocellulose film .The prepared test paper can utilize one step competition process of chromatographic type to detect out residual amount of sulfamethazine in tested sample quickly and economically.

The present invention discloses an automatic identification system and an automatic identification method for high-speed railway CPIII markers. The automatic identification system carries out image processing on an acquired image through an industrial control computer, makes features of the marker in the image more clear through algorithms such as average filtering, image differencing and image segmentation, is conductive to template matching in the next step, carries out template matching, creates a template which contains features of an object for matching, achieves the identification purpose through comparison and calculation via the template in a target image containing the object, and provides support for the subsequent center point coordinate calculation and camera calibration of the CPIII marker.

The invention discloses an electrochemical sensor for rapidly measuring carmine and citrine. The electrochemical sensor at least comprises a carbon paste electrode, wherein the carbon paste electrode comprises a casing and a copper bar, and a cavity is formed in the front end of the casing and filled with carbon paste. The invention further provides a preparing method of the sensor. The preparing method comprises the following steps: S1, dissolving Al(NO3)3.9H2O in secondary water, then adding urea, transferring an obtained solution into a hydrothermal reactor, putting in an oven to react, washing and suction-filtering solids obtained by reaction with the secondary water, drying in the oven, then putting in a muffle furnace and forging to obtain an aluminum oxide material; S2, uniformly mixing the aluminum oxide material, graphite powder and paroline oil to obtain the carbon paste, then pressing the carbon paste into a cavity at the front end of the casing, and abrading and polishing on weighing paper to make the electrochemical sensor for rapidly measuring carmine and citrine. The made electrochemical sensor is simple and convenient to operate, has great environment-friendliness and high flexibility, does not require long-time enrichment and meets the requirements of rapid detection.

The invention discloses synthesis and application of a novel fluorescent probe for rapidly detecting hydrogen peroxide, and belongs to the technical field of rapid analysis and detection of hydrogen peroxide. Based on the principle that borate can be selectively oxidized and hydrolyzed by hydrogen peroxide, 4-bromomethylphenylboronic acid pinacol ester is condensed by a hemicyanine parent skeletonto synthesize a hydrogen peroxide molecular fluorescent probe TCBOR, and the hydrogen peroxide molecular fluorescent probe TCBOR has a large conjugation and strong push-pull electronic system, so that the response to hydrogen peroxide is accelerated, and long-wavelength near-infrared fluorescence (723 nm) is emitted. The response time to hydrogen peroxide is as short as 7 min, so that the fluorescence intensity is increased by 55 times, the detection lower limit is 2.27 [mu]M, and interference of multiple ions can be resisted. The probe has good cell membrane penetrability and relatively lowbackground fluorescence and has the pH applicable range of 7.4 to 10. The probe has the characteristics of rapidness, high sensitivity, strong specificity and selectivity and the like, is applied to rapid detection of the content of hydrogen peroxide in food, has excellent indexes and completely meets the rapid detection requirement.

The invention discloses a fluorescent probe and a kit for abasic endonuclease 1 as well as application thereof. The probe is a DNA / RNA hybrid probe, wherein a DNA chain is provided with an abasic site; the 5' side of the abasic site is G base; two mismatch bases continuously exist at the 3' side to form an outgrowth region; a fluorophore group and a quenching group are respectively marked on DNA chains at two sides of the outgrowth region. APE1 can recognize and cut a phosphoester bond in the 5' direction of the abasic site on the DNA chain and divide the DNA chain into two parts, so that thefluorophore group transmits a fluorescence signal and direct detection of the APE1 content in a complex biological sample environment is realized. Compared with an existing fluorescence analysis method, the fluorescent probe disclosed by the invention has the advantages that a probe structure and a reaction solution formula are greatly improved, the sensitivity is higher, and detection results aremore accurate and more reliable; in addition, a reagent is lower in cost and better in stability.

The invention discloses a machining method and a tool for elastic check rings. The machining method comprises the following steps that spring winding is conducted; the two end faces of springs are machined, the sizes of the check rings are checked, and the unsatisfied sizes are finely calibrated; the check rings are loaded into a heat treatment clamp to be quenched and tempered, and the hardness value of the material of the check rings is 82 HR15N-86 HR15N; the flatness and the end face sizes of the check rings are finely trimmed, and heat treatment deformation is corrected; burrs are polished, so that the edges of the check rings are smooth; and all the sizes of the check rings are inspected, anti-corrosion and anti-rust treatment is conducted on the surfaces of the check rings which are inspected to be qualified, and finally packaging and delivery are conducted. The invention further provides the heat treatment clamp for the elastic check rings and the size inspection tools for the elastic check rings. Aiming at precision machining of 75Mn wire elastic check rings with the large width-to-thickness ratio, the machining method meets the requirements for the size and tolerance of the check rings, deformation is controlled, and the requirement for rapid detection during mass production is met.

The invention discloses an application of a known compound 1-amino-2-naphthol as beta-naphthol source dye hapten. An artificial antigen and a monoclonal antibody of the corresponding beta-naphtho source dye are prepared; the monoclonal antibody has good immune detection performance for various beta-naphthol source dyes according to experimental data.

The invention discloses an intellectualized and portable water quality testing meter. The front side face of a box body is provided with a display screen and a press key, a control circuit is installed in the box body and is close to the left side face of the box body, a sampling groove seat is installed in the box body and is close to the right side face of the box body, a sampling groove cover is installed on the box body and is located above the sampling groove seat, a first LED light source, a second LED light source, a third LED light source and a fourth LED light source are all installed on the control circuit, an LED with wave length being 510 nm is adopted in the first LED light source, an LED with wave length being 540 nm is adopted in the second LED light source, an LED with wave length being 640 nm is adopted in the third LED light source, and an LED light source with wave length being 700 nm is adopted in the fourth LED light source. The intellectualized and portable water quality testing meter has the advantages of being easy to operate, high in accuracy, convenient to carry and the like.

The invention provides an SERS detection method of brodifacoum or bromadiolone in edible oil. The method comprises the following steps: mixing the edible oil containing the brodifacoum or bromadiolonewith a mixed solution of ethyl acetate and chloroform, adding a saturated Na2CO3 water solution, carrying out mixing, and carrying out centrifugal separation to obtain a supernatant; and dropwise adding the supernatant to a substrate of the silver nanocube, carrying out detecting by using a Raman spectrometer, and acquiring and obtaining SERS signals of the brodifacoum or bromadiolone. The detection method has the advantages of accurate detection result, rapid detection and the like.

The invention provides an SERS (Surface Enhanced Raman Scattering) detection method for trace toxic mouse strength in vomit, which comprises the following steps: mixing a vomit extracting solution containing toxic mouse strength with an SERS substrate, carrying out SERS detection by using a Raman spectrometer, and collecting and obtaining an SERS spectral signal of toxic mouse strength. According to the SERS detection method for trace toxic mouse strength in vomit provided by the invention, after a sample is pretreated, the sample is sampled and dripped on the SERS substrate for detection, and compared with traditional toxic mouse strength detection, the SERS detection method has the advantages of accurate detection result, short time, simple operation and the like.

The invention discloses a pesticide detection method based on fluorescence analysis. According to the pesticide detection method, an organic fluorescent probe is combined with the protein to form a fluorescence detection system with a host-guest structure, and detection is realized according to the quantitative relationship between the generated fluorescence signal and the pesticide concentration by utilizing a competitive ligand replacement reaction between the pesticide with stronger binding force and the organic fluorescent probe. The pesticide detection method disclosed by the invention is low in cost, high in sensitivity and short in response time, and meets the requirement of in-vitro rapid detection; meanwhile, the fluorescence analysis method has the advantages of non-invasive detection and visualization, and is particularly suitable for carrying out in-situ detection on pesticide residues in animal and plant bodies.

The invention discloses a method for rapidly determining aluminum species in dried beancurd sticks by adopting an X-ray fluorescence spectrometer. According to the method, the 2theta value of the position of an AlK alpha second-order diffracted ray spectrum peak in each standard sample with known aluminum species is determined by the X-ray fluorescence spectrometer and taken as the basis for analysis of the aluminum species, a determined 2theta value of the position of an AlK alpha second-order diffracted ray spectrum peak in a dried beancurd stick sample is compared with the the position 2theta value of the second-order diffracted ray spectrum peak of AlKalpha in each standard sample, and the aluminum species in the dried beancurd sticks are determined. The method has the advantages of simple and rapid operation, accurate determination and capability of meeting the requirement for rapid detection of massive output dried beancurd sticks.

The invention discloses an LAMP (loop-mediated isothermal amplification) kit for rapid detection of duck plague viruses. According to the invention, a set of complete LAMP system is constructed; an LAMP amplification method can be achieved with the application of a water-bath kettle or a heat-preserving cup; results are visible to naked eyes; and the LAMP amplification method is relatively simple to operate and high in sensitivity, and the method is specially suitable for on-site quarantine at a basic level. A detection duration (including a sample processing time) is shortened from 3-4h to 2h, detection limit reaches 1pg / mL and specificity is 100%, without occurrence of false positive and false negative. With the application of the kit and a DPV pathogen PCR detection method (GB / T 22332-2008), duck plague viruses in 200 sick duck samples can be detected, and a coincidence rate of the two methods reaches 100%. The kit provided by the invention has the characteristics of being efficient, strong in specificity, high in sensitivity and the like, and a requirement of rapidly detecting samples in a large batch can be satisfied. A subsequently established LAMP diagnosis formula is applicable to on-site rapid detection and is accurate in result and convenient to use.

The invention discloses a sample pretreatment method for detecting sodium thiocyanate in liquid food, and relates to the technical field of food detection. The method comprises the following steps oftaking liquid food samples; putting the liquid food samples into a triangular flask; adding a trichloroacetic acid solution; standing after the uniform mixing; filtering to obtain supernate A for later use; adding super-pure water into the supernate A for dissolution and dilution; transferring the materials into a volume bottle; performing ultrasonic oscillation; respectively adding precipitants into the volume bottle for vortex precipitation; reaching constant volume by super-pure water; performing centrifugation treatment; sucking supernate B; using a microporous film for filtering, so as toobtain a sample solution. The method can be used for effectively removing impurities such as protein, fat and grease in the liquid food; the pretreatment is simple; the reproduction quality is high;the sample pretreatment time is shortened; after the treatment, the impurities are few; the detection accuracy and precision are high; the fast detection requirements of a great number of samples canbe met.

A method for preparing test paper of oxytetracyline colloidal gold detection technique anchors oxyterracycline coupled antigen and resisting mouse IgG oxytetracycline single resistance being labeled with colloidal gold and some other reagents on nitrocellulose film .The prepared test paper can utilize one step competition process of chromatographic type to detect out residual amount of oxytetrcycline in tested sample quickly and economically.

The invention discloses a method for identifying a dyed orange based on near infrared spectroscopy. The method comprises the following steps: (1) collecting data and establishing a qualitative model:collecting spectral data of collection points on the surface of an orange sample, dividing the spectral data into a prediction set and calibration set, and establishing the qualitative model; (2) carrying out major component analysis: performing spectral correction, determining the major component according to the cluster of the qualitative model in a corrected image, and carrying out data dimension reduction on independent variables having a correlation; and (3) identifying the dyed orange through the major component analysis. The method has the advantages of simplicity in operation and low cost.