Veritying test paper of oxytetracycline gel and production thereof

A technology for detecting test strips and oxytetracycline, which is applied in the direction of biological testing, measuring devices, material inspection products, etc., can solve the problems of long detection time, small amount of sample screening, and limited detection items of oxytetracycline, so as to meet the requirements of rapid detection Effect

Inactive Publication Date: 2006-08-16
YUNNAN UNIV +2
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  • Abstract
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Problems solved by technology

At present, the method for measuring oxytetracycline mainly contains methods such as high-performance liquid chromatography (HPLC) and enzyme-linked immunosorbent immunoassay, and HPLC method requires special instruments and equipment and professional technicians, and sample screening amount is few, needs the pretreatment of sample, therefore Its detection cost is high and the detection time is long. These factors limit the development of the HPLC method for the detection of oxytetracycline, and cannot fully meet the needs of current food safety for the detection of oxytetracycline residues.
However, enzyme-linked immunosorbent reagents mainly rely on imports, and the detection cost is relatively high.

Method used

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  • Veritying test paper of oxytetracycline gel and production thereof
  • Veritying test paper of oxytetracycline gel and production thereof
  • Veritying test paper of oxytetracycline gel and production thereof

Examples

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Embodiment Construction

[0027] 1. Oxytetracycline test paper

[0028] On the front from bottom to top are lower absorbent pad 1, gold-labeled oxytetracycline monoclonal antibody 2, oxytetracycline conjugated antigen 3, anti-mouse IgG antibody 4, upper absorbent pad 5, nitrocellulose membrane 6, and plastic liner 7 .

[0029] The surface of the lower absorbent pad 1 and the solid-phase gold-labeled monoclonal antibody 2 and the surface of the upper absorbent pad 5 are pasted with colored self-adhesive paper.

[0030] Among them, oxytetracycline-conjugated antigen 3 was used as the detection band, and anti-mouse IgG antibody 4 was used as the reference band.

[0031] The width of the film strip is not less than 2.5mm, and the surface is smooth, the material is firmly attached, and the liquid migration speed is not less than 5mm / min.

[0032] 2. Preparation of oxytetracycline test paper

[0033] (1) Combine oxytetracycline and bovine serum albumin molecules to form oxytetracycline-coupled antigen 3, ...

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Abstract

A method for preparing test paper of oxytetracyline colloidal gold detection technique anchors oxyterracycline coupled antigen and resisting mouse IgG oxytetracycline single resistance being labeled with colloidal gold and some other reagents on nitrocellulose film .The prepared test paper can utilize one step competition process of chromatographic type to detect out residual amount of oxytetrcycline in tested sample quickly and economically.

Description

Technical field [0001] The invention belongs to test paper and preparation for testing substance to be tested by immunoreaction of labeled antibody and antigen. Background technique [0002] Oxytetracyclin belongs to the tetracycline family of antibiotics, has broad-spectrum antibacterial activity, and its price is low. Therefore, it is widely used in the production of livestock and poultry meat products as a feed additive for livestock and poultry to control certain animal diseases and promote the growth and development of animals. . Because the drug residue caused by its abuse is potentially harmful to human health, its residue limit in animal meat products is strictly controlled by the Ministry of Agriculture, and the residue limit of oxytetracycline in meat products is 0.1mg / kg. At present, the method for measuring oxytetracycline mainly contains methods such as high-performance liquid chromatography (HPLC) and enzyme-linked immunosorbent immunoa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/544G01N33/558G01N33/52
Inventor 马岚袁航葛长荣张曦
Owner YUNNAN UNIV
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