61 results about "Oxytetracycline" patented technology

The invention provides an oxytetracycline-containing organic pyrite micron column and a synthesis method thereof, the oxytetracycline-containing organic pyrite micron column is prepared by preparing a solution containing potassium salt and oxytetracycline, adding ferrous salt and KFeS2 and reacting under stirring, an organic pyrite material is synthesized under a mild condition, the morphology is uniform, and the preparation method is simple. The organic sulfur iron micron column shows the reduction coordination characteristic of an oxytetracycline-Fe / S ligand, the performance stability of a Fe / S structural material is enhanced, the applicable pH range is wide, and the complexing breaking and chromium removal efficiency on organic chromium under the action of an electric field is remarkably enhanced.

The invention discloses a nucleic acid aptamer TET-HeR of tetracycline and application of the nucleic acid aptamer TET-HeR, and belongs to the technical field of nucleic acid aptamers. The nucleic acid aptamer TET-HeR of the tetracycline is a combination of an aptamer TET-A1 and an aptamer TET-C1, the nucleotide sequence of the aptamer TET-A1 is as shown in SEQ ID NO.5, and the nucleotide sequence of the aptamer TET-C1 is as shown in SEQ ID NO.7. The nucleic acid aptamer TET-HeR of the tetracycline is a combination of the aptamer TET-A1 and the aptamer TET-C1. The invention further discloses the application of the nucleic acid aptamer TET-HeR of the tetracycline in detection of the tetracycline. The affinity and dissociation constant of the nucleic acid aptamer TET-HeR of the tetracycline and the tetracycline is 62.7 nM, and the nucleic acid aptamer TET-HeR of the tetracycline has high affinity; the compound has no obvious affinity to tetracycline analogues such as aureomycin and doxycycline, has weak affinity to oxytetracycline, and has good specificity; the method can be used for specific detection of tetracycline.

The invention relates to the technical field of microbial fermentation, in particular to a fermentation medium for producing oxytetracycline. According to the fermentation culture medium, a corn starch-molasses-wheat hydrolyzed sugar composite carbon source, a soybean cake powder-corn steep liquor-yeast extract composite nitrogen source and a choline chloride-betaine-L-lysine precursor accelerant in a specific proportion are used as a core innovative system, and a form regulating agent, a defoaming agent and inorganic salt are cooperatively added. Through the synergistic effect of multiple components, the fermentation titer and yield of oxytetracycline are remarkably improved, the fermentation period is shortened, the generation of heteroacid is effectively inhibited, the mycelium form is optimized, and the oxytetracycline fermentation medium is suitable for industrial large-scale production of oxytetracycline.

This application discloses a seaweed oligopeptide fertilizer for promoting root growth and seedling development, and its production process, belonging to the field of fertilizer technology. The fertilizer, by weight, comprises: 2-10 parts seaweed oligopeptide complex, 1-8 parts seaweed extract, 10-30 parts compound amino acids, 1-10 parts blueberry pomace, 20-50 parts oxytetracycline waste decomposition products, and 30-60 parts recycled organic matter from kitchen waste. By combining seaweed oligopeptides with chitosan to form a complex, the stability of the active ingredients is improved; simultaneously, the synergistic utilization of oxytetracycline fermentation waste, kitchen waste, and blueberry pomace achieves resource utilization. Experiments show that this fertilizer significantly promotes crop root growth and seedling development.

The invention discloses a preparation method and application of nitrogen-rich biochar, and relates to the technical field of water treatment. The invention aims to solve the problem that antibiotics and heavy metals are difficult to remove at the same time in the existing nitrogen-doped biochar. The method comprises the following steps: 1, cutting soybean straws, mixing the cut soybean straws with urea and distilled water to obtain a mixture, carrying out ultrasonic oscillation on the mixture, then transferring the mixture into a container, and drying the mixture in a drying oven to obtain urea-loaded straws; and 2, putting the obtained urea-loaded straw into a tubular furnace into which nitrogen is introduced for heating, then taking out the straw, grinding, sieving, mixing the sieved material with alkali liquor, activating, cleaning, finally putting the cleaned sample into a drying oven, and drying until the mass is constant, thereby obtaining the nitrogen-rich biochar. The nitrogen-rich biochar prepared by the invention can adsorb oxytetracycline (OTC) and Cu (II) at the same time.

This invention relates to an oxytetracycline feeding and drying device, belonging to the field of oxytetracycline feeding and drying technology. It includes a mixing bottom trough block and a sealed top trough block. A mixing horizontal tube is rotatably installed on both the left and right sides of the inner wall of the mixing bottom trough block. An air-filling pipe is fixedly installed at the left end of the mixing horizontal tube, while a fixed horizontal rod is rotatably installed at the right end. A combined cylinder is provided at the end of the air-filling pipe and the end of the fixed horizontal rod. Several mixing components are provided on the wall of the fixed horizontal rod, and two connecting rods are symmetrically arranged on one side of the mixing components on the wall of the mixing horizontal tube. A scraper is fixedly installed at the end of each connecting rod near the inner wall of the mixing bottom trough block. This invention, through its mixing structure, achieves uniform material mixing while continuously scraping the inner wall of the storage container, preventing raw material adhesion and accumulation, and avoiding uneven drying or deterioration caused by localized retention, thus significantly improving the overall quality of oxytetracycline drying.

The invention discloses a method for detecting oxytetracycline (OTC) based on nitrogen-doped graphdiyne (2N-GY) electrochemical luminescence sensing. The prepared 2N-GY has an electrogenerated chemiluminescence signal, the surface of the electrode is modified with an aptamer (Apt I) of carboxylated 2N-GY and OTC, and then non-specific binding sites are closed by using BSA to obtain the modified electrode. The modified electrode is used as a working electrode, an electrochemiluminescence biosensor is constructed by continuously modifying OTC and quenching a probe (PDAs-Apt II), and quantitative analysis is carried out on the OTC by detecting the change of an electrochemiluminescence signal. According to the present invention, the two OTC aptamers (Apt I and Apt II) are adopted as the recognition molecules to construct the electrochemiluminescence biosensor so as to achieve the high-specificity and high-sensitivity detection of OTC, and can be used for the actual detection application of milk, and the novel carbon material is adopted to replace the toxic light-emitting reagent, such that the detection cost is reduced, and the safety problem existing in the traditional light-emitting system is reduced.

This invention discloses the application of *Polygonum aviculare* extract in the prevention and control of bacterial leaf spot in peach. The invention utilizes ultrasound-assisted extraction to extract the active components of *Polygonum aviculare*. Antibacterial experiments demonstrated that the extract exhibits good antibacterial activity against the pathogen of peach bacterial leaf spot (*Xanthomonas arboricola* pv. pruni, Xap), with a minimum inhibitory concentration (MIC) identical to that of the positive control drug oxytetracycline, both at 0.125 mg / mL. The antibacterial activity of *Polygonum aviculare* extract is comparable to that of oxytetracycline, making it a potential agent for the prevention and control of peach bacterial leaf spot.

The invention belongs to the technical field of prevention and control of drug-resistant bacteria, and particularly discloses a composite bactericidal composition and application thereof in preparation of a reagent for killing aeromonas hydrophila oxytetracycline drug-resistant strains. The minimum inhibitory concentration of the epsilon-polylysine to an aeromonas hydrophila oxytetracycline drug-resistant strain is as low as 3.125 g / mL. Meanwhile, the sterilization mechanism of the epsilon-polylysine on aeromonas hydrophila oxytetracycline drug-resistant strains is discussed through metabonomics research. On the basis, the application finds that various exogenous small molecule metabolites such as 2-phenylacetamide and the like are combined with epsilon-polylysine for use, so that the absorption of drug-resistant bacteria on epsilon-polylysine can be promoted, the sterilization effect of the epsilon-polylysine is effectively enhanced, and the problem of drug resistance of bacteria is solved. Therefore, when the epsilon-polylysine and the exogenous small molecule metabolite are jointly applied, compared with the existing single antibiotic therapy, the epsilon-polylysine has higher activity and better safety and operability in preparation of the reagent for resisting the aeromonas hydrophila oxytetracycline drug-resistant strain.

The invention belongs to the technical field of visible light photocatalytic materials and environmental pollutant degradation, and discloses a Bi12O17Br2 nanotube photocatalyst and a preparation method and application thereof.According to the photocatalyst, a traditional Bi12O17Br2 nanosheet is converted into a hollow nanotube structure through a surface tensile strain induced morphology regulation and control strategy, tubular morphology reconstruction is achieved through one-step synthesis, and the photocatalytic performance of the photocatalyst is improved. Compared with the prior art, the preparation method has the advantages that the visible light response and photo-induced electron-hole separation efficiency of the material are remarkably improved, the photocatalytic degradation efficiency of the material on oxytetracycline under visible light irradiation exceeds 96% within 120 min, meanwhile, excellent adsorption performance and good cycle stability are shown, and the material has important application value in the field of oxytetracycline wastewater treatment.

The present disclosure relates generally to methods and systems for controlling citrus greening disease in citrus plants, including for example orange trees.

The present disclosure relates generally to methods and systems for controlling Candidatus Liberobacter Disease in citrus plants, including, for example, orange trees. In some embodiments, the methods comprise injecting an injection formulation comprising a combination of oxytetracycline and streptomycin into a tree.

The invention discloses an ammonia nitrogen recovery and recycling method for oxytetracycline pharmaceutical wastewater, belongs to the field of pharmaceutical wastewater treatment, is used for wastewater treatment, and is characterized in that wastewater in an adjusting tank meets the following conditions: COD (Chemical Oxygen Demand) is less than or equal to 800mg / L, TDS (Total Dissolved Solids) is less than or equal to 5g / L, oxytetracycline residue is less than or equal to 10mg / L, and the initial concentration of ammonia nitrogen is 800mg / L-1200mg / L. The ammonia nitrogen recovery and recycling method comprises the steps of staged pH pretreatment, membrane contact deamination, dynamic pH regulation and control, ultrasonic antifouling and resource recovery. By means of targeted inhibition of membrane pollution, the added value of products is increased, resource utilization of wastewater is achieved, and the technical problems that in the prior art, membrane pollution control is insufficient, and the product function is single are solved.

The invention provides a method for improving the anaerobic fermentation acid production effect of oxytetracycline-containing sludge by using thiosulfate pretreatment, which comprises the following steps: adding anaerobic sludge of a sewage plant into a fermentation tank as inoculated sludge, adding oxytetracycline and thiosulfate, then blowing nitrogen to drive oxygen, sealing the fermentation tank to degrade oxytetracycline through constant-temperature anaerobic fermentation, and carrying out centrifugal separation to obtain the oxytetracycline-containing sludge. The supernatant is fermentation liquor rich in volatile fatty acid; wherein the total suspended solid concentration of the inoculated sludge is 20-27 g / L, the mass concentration of the thiosulfate in the sludge is 900 mgS / L in terms of S, and the adding concentration of the oxytetracycline is 5-25 mg / L. According to the method, sludge hydrolysis is promoted, a microbial community structure is adjusted and electron transfer is accelerated by utilizing the reducibility of thiosulfate, so that the conversion rate of organic matters to VFA is increased, oxytetracycline is synchronously and efficiently removed, and a new solution is provided for antibiotic pollution control and sludge resource utilization.

The invention relates to the technical field of wastewater treatment, and discloses an oxytetracycline wastewater treatment method based on phycomycete symbiotic granular sludge, which comprises the following steps: (1) performing electrocatalytic oxidation treatment on oxytetracycline wastewater; (2) carrying out photocatalytic treatment on the oxytetracycline wastewater subjected to the electrocatalytic oxidation treatment; and (3) treating the oxytetracycline wastewater subjected to photocatalytic treatment by adopting phycomycete symbiotic granular sludge. In the scheme of the application, through the synergistic effect of the three processes of catalytic oxidation, photocatalysis and phycomycete symbiosis, oxytetracycline can be more effectively removed, and efficient deep treatment of oxytetracycline wastewater is realized.

This invention belongs to the technical field of crystalline materials, and discloses a praseodymium-based complex crystalline material, its preparation method, and its fluorescence recognition application, with the chemical formula {[Pr(L 1.5 The compound is 2,2'-(2,3,5,6-tetramethyl-1,4-phenylene)bis(methylene)bis(sulfonyl)diethyl)benzoic acid. The preparation method involves dissolving Pr(NO3)3·6H2O, H2L, and the auxiliary uncoordinated ligand 1,4-bis(imidazol-1-yl)benzene in a DMF / ethanol / water mixed solvent, reacting at 90°C for 3 days, and then cooling to obtain plate-like crystals. This material is effective against Fe... 3+ It exhibits highly selective and sensitive fluorescence quenching response with a quenching rate of 99.8%; it can specifically recognize ethoxyquinoline (fluorescence enhancement) and 2,4-dichlorophenoxyacetic acid (fluorescence quenching); it can specifically recognize the antibiotic oxytetracycline with a fluorescence quenching rate exceeding 99%.

The invention relates to direct-fed microbials for use in E. coli inhibition in animals in combination with antibiotics selected from the group consisting of ceftiofur, oxytetracycline, tulathromycin, and lincomycin. More particularly, the invention relates to isolated Bacillus strains 2, 3, 4, 5, 9, 57, 71, and 126, and strains having all of the identifying characteristics of these strains, for a use comprising the above-mentioned use.

The invention belongs to a feed additive, and particularly relates to a preparation method of a chloro-oxytetracycline additive for feed, which comprises the following steps: step 1, spore preparation: inoculating preserved strains to a slant culture medium, culturing spores under proper temperature and humidity conditions, adding a proper amount of nutrient substances, and culturing the spores under proper temperature and humidity conditions; the method has the beneficial effects that oxytetracycline is released and impurities are removed through acidification and wall breaking of oxalic acid, the ammonia water promotes crystallization by adjusting the pH value, and the production efficiency and the product quality of oxytetracycline can be remarkably improved through combined use of the oxalic acid and the ammonia water; in addition, wastewater generated by using oxalic acid and ammonia water can be treated through a reasonable wastewater treatment process and recycling measures, so that the influence on the environment can be reduced; when the prepared oxytetracycline is used for producing chloro-oxytetracycline, the method has the advantages of raw material cost control, production process optimization, technical innovation and application, environmental protection, sustainability, market competitive advantage and the like.

The application discloses a three-dimensional positive-negative general electrode, which is used for adsorbing organic pollutants and comprises a three-dimensional skeleton and an adsorption functional layer; the three-dimensional skeleton and / or the adsorption functional layer has conductivity; the adsorption functional layer is covered on the surface of the three-dimensional skeleton, and the constituent material of the adsorption functional layer comprises one or more of conductive polymers, biochar and synthetic carbon. The electrode has large adsorption capacity and high mass transfer efficiency, can efficiently capture organic pollutants in wastewater, and overcomes the problems of traditional physical adsorbents, such as easy loss and difficult recovery, and the like; when the electrode is used as a cathode or an anode to adjust and adsorb organic pollutants under the cooperation of an electric control, the electrode has stronger adsorption capacity and enrichment capacity. The application further provides a wastewater treatment system, which can quickly and completely treat wastewater, and provides applications of the three-dimensional positive-negative general electrode and the wastewater treatment system in treatment of organic pollutants, such as oxytetracycline, methylene blue, methyl orange and ciprofloxacin.

The invention discloses an oxytetracycline mushroom dreg treatment and utilization method. The method comprises the following steps: firstly, dehydrating mushroom dregs, mixing the dehydrated mushroom dregs with an acidic solution with the pH value of 3.0-4.0 in proportion, and carrying out drug removal pretreatment at 50-60 DEG C to destroy mycelia and degrade oxytetracycline; carrying out solid-liquid separation after pretreatment to obtain reagent-removed solid residues, and carrying out homogenizing and size mixing until the pH value is 6.5-7.5; then adding compound protease composed of neutral protease and alkaline protease according to the mass ratio of 1: 1-1: 2 for directional enzymolysis; after the enzymatic hydrolysate is subjected to solid-liquid separation, supernate is sequentially subjected to multi-stage membrane separation and purification steps such as microfiltration / ultrafiltration, 10kDa and 1kDa ultrafiltration membrane combination grading, nanofiltration desalination concentration and the like, and finally, a protein peptide product is obtained through drying. Optionally, a decoloring refining step may be added after nanofiltration. The invention also relates to a protein peptide product which is prepared by the method and has the oxytetracycline residue lower than 0.1 mg / kg and the molecular weight mainly distributed in 1-10kDa, and application of the protein peptide product. The method realizes high-value utilization of hazardous wastes, and has environmental and economic benefits.

The present application relates to the technical field of microbial fermentation, and particularly relates to a fermentation medium for producing terramycin. The fermentation medium takes corn starch-molasses-wheat hydrolysis sugar composite carbon source, bean cake powder-corn syrup-yeast extract composite nitrogen source and choline chloride-betaine-L-lysine precursor promoter as a core innovative system in a specific proportion, and further adds a morphological regulator, a defoaming agent and inorganic salts. Through the synergistic effect of multiple components, the fermentation titer and yield of terramycin are significantly improved, the fermentation period is shortened, the generation of mixed acids is effectively inhibited, and the mycelial morphology is optimized, so that the fermentation medium is suitable for industrial large-scale production of terramycin.

The invention relates to the technical field of aquaculture, in particular to a hippocampus kelloggi larva zoozoonosis prevention and treatment method which comprises the following steps: adding formalin for soaking for 12 hours on the first day, and then running water every other day; on the third day, formalin is added for soaking for 12 h, and then water flows for one day; on the fifth day, formalin and methylene blue are added to be soaked for 12 h, and then water flows for one day; respectively adding thioether floxacin on the seventh day and the eighth day, and standing; according to the method, insect killing, mildew prevention and sterilization are combined, parasites such as synechozostomia can be killed, secondary infection such as water mildew and bacteria can be prevented, the survival rate of the seahorse fry within 20 days old is remarkably increased, and the breeding efficiency is improved.

The invention discloses a long-acting oxytetracycline injection and a preparation method thereof in the technical field of veterinary drugs, each 100ml of the injection contains 10-30g of oxytetracycline, 10-30ml of a composite organic solvent, 0.1-0.5 g of a sustained release agent, 0.05-0.1 g of an antioxidant, 1.5-3g of a complexing agent and 0.01-0.02 g of an analgesic, the pH value is regulated to 8.0-8.5 by using a pH regulator, and the volume is fixed to 100ml by using water for injection; the composite organic solvent is a mixture of alpha-pyrrolidone, dimethylformamide and dipropylene glycol monomethyl ether. According to the long-acting oxytetracycline injection, oxytetracycline is adopted as a main material, lidocaine hydrochloride is added to serve as an analgesic, polyvinylpyrrolidone K12 or poly-racemic lactic acid is added to serve as a slow-release agent, the animal dysentery treatment effect, medication compliance and treatment cycle of the prepared long-acting oxytetracycline injection are obviously improved, the effect is prominent, and in addition, the long-acting oxytetracycline injection has a good application prospect. A composite organic solvent system compounded by alpha-pyrrolidone, dimethylformamide and dipropylene glycol monomethyl ether is adopted in the injection, so that the stability of the injection is improved, and the drug effect of the oxytetracycline injection is well guaranteed and prolonged.

The present disclosure provides a gene combination for expressing and producing terrequinone A in Escherichia coli and use thereof. The gene combination includes a tdiAS gene, a tdiBS gene, a tdiCS gene, a tdiDS gene, a tdiES gene, an sfpS gene, an ScCKS gene, and an AtIPKS gene with nucleotide sequences set forth in SEQ ID NOS:1 to 8. In the present disclosure, a recombinant engineered strain capable of producing terrequinone A having anti-cancer activity is obtained by separately constructing recombinant plasmids pC02 and pU03 through the eight genes and transforming the two recombinant plasmids into E. coli. The content of terrequinone A in a fermentation broth thereof is 106.3 mg / L, which has potential application value in the biopharmaceutical field.

The invention discloses a fluorescent carbon nanocluster taking polyethylene glycol as a template and having blue fluorescence emission capability as well as a synthesis method and application of the fluorescent carbon nanocluster, and belongs to the technical field of chemical synthesis and biological analysis and detection. The fluorescent carbon nanocluster is obtained by stacking dozens of xylose molecules on a polyethylene glycol template molecule through a simple hydrothermal synthesis method, has good water solubility and chemical stability, and has a molecular structure and fluorescence emission capability similar to those of a metal fluorescent nanocluster; the problem of heavy metal leakage of a traditional metal fluorescent nano-cluster probe is solved, so that the metal fluorescent nano-cluster probe has higher safety and biocompatibility, and has a wide application prospect in the fields of fluorescence sensing, biological imaging, environmental analysis and the like. Based on the fluorescence enhancement effect of an antibiotic drug oxytetracycline on the fluorescent carbon nano-cluster probe, high-sensitivity analysis and detection on the oxytetracycline can be realized.

The invention relates to an inonotus obliquus fermentation product and application of the inonotus obliquus fermentation product in hypoglycemic drugs, and belongs to the technical field of inonotus obliquus fermentation products. A component culture medium is prepared from 10-15 parts of plant flour, 3-6 parts of glucose, 0.8-1.2 parts of dipotassium phosphate, 1-2 parts of magnesium sulfate, 5-8 parts of protein peptide, 4-8 parts of agar and 60-80 parts of purified water; the component B culture medium is prepared from the following raw materials in parts by weight: 12-15 parts of plant starch, 2-4 parts of glucose, 1-3 parts of oxytetracycline, 1-3 parts of chloramphenicol, 8-16 parts of sodium hyaluronate and 60-80 parts of purified water. Through the multi-target synergistic effect of improving insulin resistance, protecting islet cells, inhibiting sugar absorption and the like, the hypoglycemic effect is obvious, and the prepared inonotus obliquus fermentation product is simple in process, low in operation difficulty, suitable for industrial production and capable of being applied to the fields of cosmetics, textile industry and medicine.

This invention discloses the application of *Prunus cerasifera* leaf extract in the prevention and control of bacterial leaf spot disease in peaches. The invention utilizes ultrasound-assisted extraction to extract the active ingredients from *Prunus cerasifera* leaves. Antibacterial tests demonstrate that the 80% ethanol extract of *Prunus cerasifera* leaves exhibits good antibacterial activity against the pathogen of peach bacterial leaf spot disease (*Xanthomonas arboricola* pv. pruni, Xap). The minimum inhibitory concentration (MIC) is the same as that of the positive control drug oxytetracycline, both being 0.125 mg / mL. Therefore, it can be used as a raw material for the prevention and control of peach bacterial leaf spot disease.

The invention discloses a kind of fluorescence analysis method for detecting oxytetracycline in water sample, specifically including the preparation of sulfur quantum dot:Sequentially by 4.80mL, 0.10mol / L thioacetamide, 8.50mL H at room temperatureO and 3.00mL PEG‑400 are added in reaction vessel and stirred and mixed, then 3.70mL, 3wt% hydrogen peroxide are added dropwise to be fully mixed, and then sulphur quantum dot is obtained at 130 DEG C of stirring reactions 4.5h;Steps such as the drawing of standard curve and the determination of oxytetracycline in water sample to be measured.The present invention is based on the high selectivity fluorescence quenching effect of oxytetracycline, proposes a simple, low-cost, highly sensitive oxytetracycline fluorescence determination system, and is successfully applied to the determination of oxytetracycline in water sample.

The invention discloses a carbon quantum dot / indium sulfide ohmic junction and a preparation method and application thereof, and the method comprises the steps: 1, taking 0.5-0.9 mmol of InCl3. 4H2O and 1-1.8 mmol of thioacetamide according to a molar ratio of 1: 2, adding the taken InCl3. 4H2O and thioacetamide into 70mL of ultrapure water, and stirring to obtain a solution B; step 2, adding 1.467-5.705 mg of carbon quantum dots into the solution B, and stirring to obtain a mixed solution C; and step 3, pouring the mixed solution C into a high-pressure kettle with a polytetrafluoroethylene lining, reacting at 160-179 DEG C for 8-10 hours, naturally cooling to room temperature, and sequentially washing and drying to obtain the carbon quantum dot / indium sulfide ohmic junction. The preparation method has the advantages of being simple in preparation process, low in cost and environmentally friendly, and the synthesized carbon quantum dot / indium sulfide ohmic junction has excellent photocatalytic performance and can effectively degrade oxytetracycline and sulfamethoxazole in wastewater.