28 results about "Tissue extracts" patented technology

The present invention relates to a method for using a lyophilization hyaline cartilage powder to produce a composition for regenerating cartilage, and a composition for regenerating cartilage produced by using the method, the method comprising: A) a step for preparing hyaline cartilage; B) a step for freeze-drying and crushing the hyaline cartilage, and producing a lyophilization hyaline cartilage powder; C) a step for producing an adipose tissue extract from autologous adipose tissue; and D) a step for producing a composition which is for regenerating cartilage and including the lyophilization hyaline cartilage powder and the adipose tissue extract.

The invention belongs to the field of cosmetics, and discloses an anti-wrinkle soothing composition, application of the anti-wrinkle soothing composition and a cosmetic. Comprising the following components in parts by weight: 15-20 parts of hydroxypropyl tetrahydropyrantriol, 10-15 parts of tetrahydromethylpyrimidine carboxylic acid, 5-10 parts of a leukocyte extract, 5-20 parts of a leontopodium alpinum callus extract solution and the balance of a bifidus yeast fermentation product filtrate, through reasonable compounding of the five components, synthesis of collagen and elastin can be effectively promoted, and good anti-wrinkle and soothing effects are achieved.

The present invention relates to an extract of in vitro cultured C. sativa root tissues, characterized in that said extract comprises at least one lignanamide. The present invention also relates to a pharmaceutical, dermatological, nutraceutical, or cosmetic composition comprising, as an active ingredient, the extract described above.

The invention belongs to the technical field of cosmetics, and discloses a saussurea involucrate callus extract as well as a preparation method and application thereof. The preparation method of the saussurea involucrata callus extract comprises the following steps: inoculating saussurea involucrata into an induction culture medium for culturing to obtain saussurea involucrata callus; inoculating the saussurea involucrata callus into a growth culture medium for culturing, and centrifuging to obtain saussurea involucrata suspension cells; and mixing the saussurea involucrata suspension cells with an extraction solvent, and performing high-pressure homogenization to obtain the saussurea involucrata callus extract. According to the preparation method disclosed by the invention, callus culture, high-pressure homogenization and the deep-eutectic solvent are combined, the efficiency of obtaining active ingredients in the saussurea involucrata callus is improved through a green process, and the saussurea involucrata callus extract with a remarkable anti-inflammatory effect is prepared.

The invention relates to an active pharmaceutical ingredient for repairing oral mucosa and a preparation method of the active pharmaceutical ingredient, and belongs to the technical field of biological medicine, and the active pharmaceutical ingredient comprises an amanita aurantiaca mycelium active peptide-myrobalan polyphenol composite entrapment substance, a dendrobium nobile callus extract, sorbitol, menthol, a gel matrix and the like. The amanita aurantiaca mycelium active peptide-myrobalan polyphenol composite entrapment substance is obtained by entrapment of a compound of amanita aurantiaca mycelium active peptide and myrobalan polyphenol through a sodium alginate-dopamine grafted copolymer; the amanita aurantiaca mycelium active peptide is obtained by performing common enzymolysis on the mycelium of amanita aurantiaca through bromelain and bacillus subtilis protease. The dendrobium nobile callus extract is obtained by inducing slices of dendrobium nobile seedling leaves with 2, 4-D and 6-BA, extracting and purifying. The gel matrix is prepared from modified konjac glucomannan and carboxymethyl chitosan. All the components form an anti-inflammatory, proliferative and remodeled closed-loop repair system, and the anti-degradation stability and the adhesion long-term effect are improved.

The application discloses a purification method of plant exosome, and belongs to the technical field of biological separation. The method comprises the following steps: obtaining plant juice or tissue extract, pre-treating the plant juice or tissue extract through coarse filtration and centrifugation, incubating the plant juice or tissue extract with magnetic microspheres modified with carboxyl or epoxy groups in a buffer containing calcium ions, capturing the complex by using an external magnetic field, washing the complex, eluting the complex by using a competitive elution buffer containing N-acetylglucosamine, and further purifying the complex by using a size exclusion chromatography column to obtain the plant exosome. The exosome is specifically captured by the magnetic microspheres, and the double purification strategies of competitive elution and size exclusion chromatography are combined, so that the recovery rate and purity of the exosome are significantly improved. The method is simple in operation, short in time consumption, good in repeatability, and suitable for the large-scale preparation of exosomes of various plant sources. The plant-derived exosome prepared by the method can be applied to the fields of drug delivery systems, cosmetics, respiratory health and sensory health product development.

The invention relates to the field of plant biotechnology and daily chemical products, and particularly discloses a culture method of rhizoma nardostachyos calluses and application of the rhizoma nardostachyos calluses in preparation of hair care products. The rhizoma nardostachyos callus extract obtained through the culture method has the effects of promoting hair growth, preventing alopecia and regulating scalp grease secretion, and the effect of the rhizoma nardostachyos callus extract is remarkably superior to that of a traditional rhizoma nardostachyos medicinal material extract. The rhizoma nardostachyos callus extract can be used as a novel hair care raw material for replacing a traditional rhizoma nardostachyos medicinal material, and has the characteristics of sustainability and environmental protection.

The invention discloses a method for purifying plant exosomes, which belongs to the technical field of biological separation and comprises the following steps: acquiring plant juice or tissue extracting solution, performing rough filtration and centrifugal pretreatment, incubating the plant juice or tissue extracting solution and magnetic microspheres of which the surfaces are modified with carboxyl groups or epoxy groups in a buffer solution containing calcium ions, capturing a compound by using an external magnetic field, and purifying the plant exosomes. After cleaning, eluting by adopting a competitive elution buffer solution containing N-acetylglucosamine, and finally further purifying by virtue of a size exclusion chromatographic column, so as to obtain the plant exosome. The exosome is specifically captured through the magnetic microspheres, the recovery rate and purity of the exosome are remarkably improved by combining a dual purification strategy of competitive elution and size exclusion chromatography, operation is easy and convenient, consumed time is short, repeatability is good, and the method is suitable for large-scale preparation of exosomes of various plant sources; the plant-derived exosome prepared by the method can be applied to the fields of product development of drug delivery systems, functional foods, cosmetics, respiratory health and sensory health and the like.

The present application relates to a kind of active pharmaceutical ingredients of repairing oral mucosa and preparation method thereof, belong to biological medicine technical field, and drug includes orange cover gonggao mycelium active peptide- Terminalia polyphenol composite package, dendrobium nobile callus extract, sorbitol, menthol, gel matrix etc.Orange cover gonggao mycelium active peptide- Terminalia polyphenol composite package is the complex of orange cover gonggao mycelium active peptide and Terminalia polyphenol by sodium alginate-dopamine graft copolymer package;Orange cover gonggao mycelium active peptide is the mycelium of orange cover gonggao mushroom by bromelain and bacillus subtilis protease common enzymolysis obtains.Golden lace dendrobium callus extract is the section of golden lace dendrobium seedling leaf by 2,4-D and 6-BA induction, extraction, purification and obtains.Gel matrix is prepared by modified konjac glucomannan and carboxymethyl chitosan.Each component forms anti-inflammatory, proliferation and remodeling closed-loop repair system, and improves the stability of anti-degradation and adheres long-acting.

The invention relates to an application of a saussurea longifolia callus extract in the aspects of preparing a skin external preparation, a beautifying oral preparation, a health care product, food or a food additive and the like, and the saussurea longifolia callus extract has excellent effects of resisting aging, improving sleep and enhancing immunity. Meanwhile, the composition also has multiple effects of relieving, repairing, whitening, resisting inflammation, tightening, resisting wrinkles, moisturizing, removing acnes, resisting oxidation, preventing hair loss, caring hair, caring scalp and the like, and can be used as an active additive to be applied to skin and hair care products and foods. The saussurea longifolia callus cells are used as the raw material, so that the method has the advantages of infinite proliferation, stable quality among batches, short culture period, environmental friendliness and easiness in batch culture in unrelated seasons, can be used for production under the condition of not damaging plants, and has great value and significance for sustainable development and utilization of plant resources.

The invention discloses a method for remarkably improving the rooting rate of clustered buds of citral-type camphor trees, which comprises the following steps: cutting a 2-3cm stem section with a stem tip, and transferring the stem section into a pre-culture medium; transferring into a rooting culture medium; adding plant hormones, observing the growth condition of the adventitious roots, and counting the inductivity, the average root length and the average root number of the adventitious roots. By means of the technology of anti-oxidation treatment on clumpy bud incisions, the technology of culture medium improvement, culture condition optimization, the technology of adding a plant root tip tissue extracting solution and the like, plant tissue browning is effectively reduced, the rooting rate of the citral-type camphor tree is increased, the rooting culture process of the citral-type camphor tree tissue culture seedlings is remarkably improved, and the rooting rate of the citral-type camphor tree tissue culture seedlings is increased. The citral-type camphor tree cluster bud rooting seedling with a developed and strong root system is obtained, a technical support is provided for conservation and propagation of the tree species, the quality of the seedling is ensured, the resource basis and quality of the citral-type camphor tree are improved, and healthy and sustainable development of the citral-type camphor tree and the natural citral industry is promoted.

The invention relates to a hair repairing composition containing a keratin bonding agent and application of the hair repairing composition. The composition comprises the following components: the keratin bonding agent Replex, an amaranthus caudatus seed extract, a southern semen cuscutae seed extract and a leontopodium alpinum callus extract. Wherein the mass ratio of the keratin bonding agent Replex to the amaranthus caudatus seed extract to the southern semen cuscutae seed extract to the leontopodium alpinum callus extract is 1: (0.1-0.5): (0.1-0.5): (0.5-2); according to the hair repairing composition disclosed by the invention, organic silicon or cationic regulators are not added, and aiming at three dimensions of'hair interior (keratin)-surface layer (hair cuticle / lipid)-external protection ', through the cooperation of the four components, the problem that the hair is damaged is solved, the risk of re-damage is prevented, and the hair repairing composition has the advantages that the hair repairing effect is good, and the hair repairing effect is good. And finally, the toughness of the hair is enhanced, the smoothness is improved, the luster is recovered and the breakage is reduced.

The invention relates to an application of a snow rabbit callus extract in the aspect of preparing a skin external preparation, a beautifying oral preparation, a health care product, food or a food additive, and is characterized in that the snow rabbit callus extract has better anti-inflammatory, soothing and whitening effects, and can be used for preparing the skin external preparation, the beautifying oral preparation, the health care product, the food or the food additive. Meanwhile, the composition also has the effects of repairing, tightening, resisting wrinkles, resisting oxidation, promoting autophagy, preserving moisture, removing acnes, resisting aging, enhancing immunity, resisting saccharification and the like, and can be applied to skin and hair care products and health-care foods as an active additive. The method has the advantages that the cells of the calluses of the snow rabbits are used as raw materials, the method has the advantages of infinite proliferation, stable quality of each batch, short culture period, environmental friendliness and easiness in batch culture in unrelated seasons, can be produced under the condition of not damaging plants, and has great value and significance for sustainable development and utilization of plant resources.

The application belongs to the technical field of cosmetics, and discloses a composition with instant soothing effect, a preparation method and application thereof. The composition comprises components: hydroxyphenylpropionamide benzoic acid and / or mother chamomile flower oil, farnesol and / or hydroxyl asperosaponin, kava pepper leaf / root / stem extract and / or menthol lactate, and edelweiss callus extract. The composition has significant soothing effect and can instantly soothe, thereby instantly relieving the uncomfortable feeling such as stinging, burning, swelling and itching, through reasonable compounding of hydroxyphenylpropionamide benzoic acid and / or mother chamomile flower oil, farnesol and / or hydroxyl asperosaponin, kava pepper leaf / root / stem extract and / or menthol lactate, and edelweiss callus extract.

The invention discloses a saussurea lancea callus proliferation culture medium, a saussurea lancea callus extract, a preparation method and application. The saussurea lancea callus proliferation culture medium comprises the following components: a basic culture medium for plant tissue culture and 3-100 ppm of nisin z. Compared with an existing saussurea lancea culture medium, the saussurea lancea callus proliferation culture medium can solve the technical problem that the saussurea lancea callus is prone to browning during large-scale production, and meanwhile the saussurea lancea callus proliferation coefficient can be increased, and the content of active ingredients in an extract can be increased; the saussurea lancea callus with high multiplication coefficient and the saussurea lancea callus extract with improved oxidation resistance are obtained.

The invention belongs to the field of electrochemical sensors, and provides a construction method and application of an electrochemical sensor for detecting the pH of brain glioma, and the specific process is as follows: firstly, a bimetallic MOF (Zr-TCPP (Fe)) with a heme-like structure (containing Fe element and porphyrin ring) is synthesized, and the material has electrochemical response to pH; 12 sulfydryl dodecyl phosphoric acid (12MA) is fixed on the surface of the gold electrode through an Au-S bond. Furthermore, the MOF material is immobilized on the surface of the electrode by using a Zr-O-P bond between 12MA and Zr-TCPP (Fe). The method can be applied to pH detection of the brain glioma tissue extracting solution.

The invention relates to the field of cosmetics, in particular to an astragalus membranaceus callus extract, a preparation method thereof and cosmetics. The method for preparing the astragalus membranaceus callus extract comprises the following steps: culturing under a long-day condition to obtain astragalus membranaceus calluses; the method comprises the following steps: extracting astragalus membranaceus calluses by taking propylene glycol as a solvent; wherein the astragalus membranaceus callus is obtained by inoculating the astragalus membranaceus callus to an induction culture medium for induction, and the induction culture medium comprises 6-benayl aminopurine, naphthylacetic acid and 2, 4-dichlorphenoxyacetic acid; based on the total volume of the induction culture medium, the concentration of the 6-benayl aminopurine is 0.5-2.0 mg / L; the concentration of the naphthylacetic acid is 0.5-2.0 mg / L; the concentration of the 2, 4-dichlorphenoxyacetic acid is 0.1 mg / L to 1.0 mg / L. According to the technical scheme, the content and quality of active ingredients are remarkably improved. The obtained extract has relatively strong antioxidant and whitening activity, is free of other organic solvent residues, is clear in color, and has a relatively good prospect as a cosmetic raw material.

The invention relates to the technical field of cosmetics, in particular to an NAD + composite plant extract composite liquid and a preparation method and application thereof.The NAD + composite plant extract composite liquid is prepared from, by weight, 3-6 parts of nicotinamide adenine dinucleotide, 10-12 parts of leontopodium alpinum composite extract, 6-8 parts of resina draconis extract and 6-8 parts of centella asiatica extract. The composition is prepared from the following components in parts by weight: 3 to 5 parts of hyaluronic acid modified aloe polysaccharide, 3 to 5 parts of modified chitosan, 2 to 6 parts of zinc glycinate, 1 to 2 parts of astaxanthin, 1 to 2 parts of green tea polyphenol, 2 to 5 parts of 3, 4-diaminodiphenyl ether, 1 to 3 parts of resveratrol, 1 to 3 parts of quercetin, 1 to 3 parts of apigenin and 1 to 3 parts of black pepper pigment. The leontopodium alpinum composite extract is composed of a leontopodium alpinum flower / leaf extract, a leontopodium alpinum callus extract and a leontopodium alpinum yeast fermentation lysate filtrate according to a weight ratio of (3-5): (1-2): 1. According to the compound liquid disclosed by the invention, the bioavailability and the stability of NAD + can be improved by matching and combining the raw materials, and the anti-aging effect of the compound liquid in cosmetics is improved.

This invention discloses a soothing, penetration-promoting, and moisturizing lip composition, its preparation method, and its application, belonging to the field of cosmetic technology. The composition, by weight, comprises 50.0-80.0 parts of base oil, 1.0-5.0 parts of ginger root oil, 0.2-1.0 parts of *Edelweiss* callus extract, 0.1-1.5 parts of bisabolol, 2.0-5.0 parts of polyglycerol-3 diisostearate, and 0.5-5.0 parts of hydrogenated lecithin. The components synergistically enhance each other, achieving excellent comprehensive performance in transdermal penetration promotion, moisturizing, and soothing. The composition is non-irritating to the lip mucosa, contains no traditional preservatives, and has good safety performance. The preparation process of this composition is simple, suitable for industrial production, and can be used to prepare various lip cosmetics.

The invention relates to an application of a saussurea obtusifolia callus extract in preparation of a skin external preparation, a beautifying oral preparation, a health care product, food or a food additive. The saussurea obtusifolia callus extract has excellent moisturizing, soothing and repairing effects, also has multiple effects of resisting aging, whitening, resisting wrinkles, tightening, removing acnes, resisting oxidation, preventing hair loss, protecting hair, improving sleep, enhancing immunity, improving autophagy ability and the like, and can be applied to skin and hair care products and health-care foods as an active additive. The saussurea obtusifolia callus cells are used as raw materials, so that the method has the advantages of infinite proliferation, stable quality among batches, short culture period, environmental friendliness and easiness in batch culture in unrelated seasons, can be produced under the condition of not damaging plants, and has great value and significance for sustainable development and utilization of plant resources.

The invention relates to the technical field of daily chemicals, and discloses a skin anti-aging composition as well as a preparation method and application thereof. The invention provides a skin anti-aging composition and a preparation method thereof. Comprising the following raw materials in parts by weight: 1 to 20 parts of hydroxypropyl tetrahydropyrantriol, 1 to 10 parts of vaccinium myrtillus fruit extract, 0.1 to 5 parts of tetrahydromethylpyrimidine carboxylic acid, 0.5 to 1.5 parts of paeonia lactiflora root extract, 0.01 to 5 parts of leontopodium alpinum callus extract, 0.01 to 5 parts of crithmum maritimum callus culture filtrate, 0.001 to 4 parts of ergothioneine and 0.1 to 20 parts of animal umbilical cord extract. According to the composition, various extracts and active ingredients are compounded, so that the composition has a synergistic effect, can directly reach the corium layer of skin, improves cell energy metabolism, improves mitochondrial functions, effectively removes free radicals of mitochondria, improves collagen degradation, and improves skin aging through a dual-channel of mitochondrial activation and extracellular matrix repair.

This invention belongs to the field of cosmetic technology and discloses an extract of *Saussurea involucrata* callus tissue, its preparation method, and its application. The preparation steps of the *Saussurea involucrata* callus tissue extract include: inoculating *Saussurea involucrata* in an induction culture medium to obtain *Saussurea involucrata* callus tissue; inoculating the *Saussurea involucrata* callus tissue in a growth culture medium, centrifuging to obtain *Saussurea involucrata* suspension cells; mixing the *Saussurea involucrata* suspension cells with an extraction solvent, homogenizing under high pressure to obtain the *Saussurea involucrata* callus tissue extract. This invention combines callus tissue culture, high-pressure homogenization, and a eutectic solvent, improving the efficiency of obtaining active ingredients from *Saussurea involucrata* callus tissue through a green process, and preparing a *Saussurea involucrata* callus tissue extract with significant anti-inflammatory effects.

The invention discloses a freeze-dried reagent ball special for streptococcus agalactiae CRISPR detection aiming at a tilapia mossambica tissue matrix and application of the freeze-dried reagent ball, and belongs to the field of molecular diagnosis technology and on-site rapid detection of aquatic diseases. The freeze-drying reagent ball is a three-layer spherical freeze-drying body comprising an inner core layer, an isolating layer and a shell layer, the inner core layer contains an RAA amplification component aiming at a streptococcus agalactiae cfb gene, the shell layer contains a CRISPR-Cas12a detection component and a special anti-inhibition buffer system, and the isolating layer is a solid freeze-drying material layer of which the dissolution rate is slower than that of the shell layer. Through the dissolution time sequence control of the three-layer structure, the automatic sequence that an outer shell layer firstly dissolves and neutralizes an inhibitor and presets a detection system and an inner core layer then dissolves and starts amplification is realized, and multi-step reaction is integrated into one-step closed tube operation. During detection, only tilapia tissue crude extract needs to be added, and a result is interpreted through fluorescence after constant-temperature incubation. The method effectively overcomes the problems of aquatic product tissue matrix inhibition, unstable operation of an existing single-tube method and on-site pollution risk, and is high in sensitivity, extremely simple to operate and stable at normal temperature.

The invention belongs to the technical field of skin care products, and particularly relates to a wrinkle-removing moisturizing skin care composition as well as a preparation method and application thereof. The composition is prepared from the following raw materials in percentage by mass: 0.25 to 0.3 percent of dipeptide diaminobutyrylbenzyl amide diacetate, 0.5 to 0.8 percent of oligopeptide-6, 10 to 20 percent of leontopodium alpinum callus extract, 60 to 80 percent of glycerol and the balance of water. In the culture process of the leontopodium alpinum callus, nomilin, cinnamaldehyde and other components are innovatively added into an induced differentiation culture medium. Test results show that the content of the total flavonoids in the leontopodium alpinum callus can be effectively increased through the synergistic effect of the nomilin and the cinnamaldehyde, and the content of the total flavonoids in the leontopodium alpinum callus extract reaches 26.7% or above. The content of total flavonoids in the leontopodium alpinum callus extract is increased, so that the moisturizing and wrinkle-removing effects of the skin care product are remarkably improved.

The application provides a tissue extract for inducing settlement metamorphosis of sea squirt larvae, and belongs to the field of amino acid substance functions. Sea squirt or shellfish tissue is ground and centrifuged to obtain supernatant; trichloroacetic acid is added and the mixture is placed at low temperature; the supernatant is then centrifuged; the supernatant after centrifugation is evaporated, and the obtained precipitate is dissolved in a solvent to obtain the tissue extract. The tissue extract can be applied to induce settlement metamorphosis of sea squirt larvae.

The invention discloses a day and night separate care product for relieving atopic dermatitis. The day and night separate care product comprises a daily emulsion and a night face cream which are matched for use, the daily emulsion comprises the following components in percentage by weight: 0.2-5% of an anti-irritation component and 0.2-5% of a first micro-ecological component, the night face cream is prepared from the following components in percentage by weight: 0.02 to 3 percent of itching relieving component and 0.02 to 3 percent of second micro-ecological component; wherein the anti-irritation component comprises panthenol, tetrahydromethylpyrimidine carboxylic acid, leontopodium alpinum callus extract and wild soybean seed extract; the first micro-ecological component comprises lactobacillus / soybean milk fermentation product filtrate, a chlorella extract, a lactobacillus fermentation product and beta-glucan; the itching relieving component is prepared from hydrolyzed sericin, hydroxyphenyl propionamide benzoic acid and ascorbyl palmitate; the second micro-ecological component comprises a chlorella extract and a lactobacillus fermentation product. The day and night separate protection product provided by the invention has the characteristics of synergistic interaction, multi-target generalized barrier repair, accurate time sequence and compliance with AD circadian rhythm.

The invention relates to a sensitive skin anti-aging composition containing arenaria kansuensis callus extract as well as a preparation method and application of the sensitive skin anti-aging composition, and belongs to the technical field of cosmetics. The sensitive skin anti-aging composition comprises an arenaria kansuensis callus extract, phytosphingosine, a selaginella tamariscina extract and a hydrolyzed gardenia extract. According to the preparation method, the selaginella tamariscina extract is prepared by adopting a supramolecular extraction technology, so that the active substances of the selaginella tamariscina extract are mildly and efficiently enriched, and the biocompatibility and the transdermal penetration capacity of the extract are remarkably improved. When the arenaria kansuensis callus extract is compounded with the phytosphingosine, the selaginella tamariscina extract and the hydrolyzed gardenia extract, a synergistic effect can be generated, and systematic intervention is performed on a complex skin aging network from four aspects of energy metabolism, barrier repair, cell protection and inflammation regulation; the invention provides a complete technical scheme with mild characteristic and multi-dimensional anti-aging effect for people with sensitive skin.

The present invention relates to a method for using a lyophilization hyaline cartilage powder to produce a composition for regenerating cartilage, and a composition for regenerating cartilage produced by using the method, the method comprising: A) a step for preparing hyaline cartilage; B) a step for freeze-drying and crushing the hyaline cartilage, and producing a lyophilization hyaline cartilage powder; C) a step for producing an adipose tissue extract from autologous adipose tissue; and D) a step for producing a composition which is for regenerating cartilage and including the lyophilization hyaline cartilage powder and the adipose tissue extract.