173 results about "Tissue extracts" patented technology

The present invention features use of evanescent field sensing to detect or evaluate unpurified, unlabeled analytes in raw samples. Capture molecules capable of binding to an analyte of interest can be immobilized to a sensing surface of an evanescent field sensor. The interaction between the analyte of interest in a raw sample and the immobilized capture molecules alters the refractive index at the sensing surface, thereby producing a detectable signal in the reflected light from the surface. Raw samples amenable to the present invention include, but are not limited to, cell lysates, tissue extracts, bodily fluid, or biologically-derived samples. Evanescent field sensors suitable for the present invention include, but are not limited to, grating-coupled waveguides.

A porcine adipocyte-specific polypeptide, termed leptin, is expressed in the fat tissue of pigs. Expression may be altered in over fat pigs, or expression may be in the form of a protein of lesser biological activity relative to that of leaner pigs. The porcine adipocyte polypeptide, DNA and RNA molecules coding therefor, methods for its preparation, and antibodies specific for the polypeptide are disclosed. Methods for determining the susceptibility of a pig to fat deposition are based on measuring the levels of the porcine adipocyte polypeptide in a biological fluid or tissue extract or by measuring mRNA encoding the porcine adipocyte polypeptide in cells of the subject. Methods of evaluating an agent related to the deposition of fat in swine comprise contacting the agent with an adipocyte in vitro and measuring the amount of the porcine adipocyte polypeptide or mRNA that is produced by the adipocyte. Methods of limiting fat deposition include administering porcine leptin or porcine leptin DNA, and methods of regulating intake include administering porcine leptin, porcine leptin DNA, or an antibody directed against porcine leptin.

Antibodies are described, which are suitable for identification of gamma-carboxyglutamic acid (Gla), said antibodies having an ability of identifying Gla residues in proteins and / or peptides, while not reacting with glutamic (Glu) residues in corresponding proteins and / or peptides. Methods for the preparation and identification of said antibodies as well as methods for detection of Gla in biological fluids, tissue extracts, tissue specimens, in which methods said antibodies are used, are also described. There is also described the use of said antibodies for immunopurification of Gla-containing proteins or peptides by, for instance, immunoprecipitation or immunoaffinity chromatography.

The invention discloses a test kit of HSP70 double antibody sandwich method used in the sample drawn from human, big mice and small mice, and comprises a kit body, an enzyme-labeled plate of solidified HSP70 monoclonal antibody provided in the kit body and substances provided in the kit body; the substances provided in the kit body comprises a HIS-HSP70 protein standard reagent, a HSP70 polyclonal antibody labeled by biotin, a sample dilution solution, a cleaning buffer solution, an antibody solution, a horseradish enzyme-labeled avidin, a horseradish enzyme-labeled avidin dilution solution, a substrate visualization solution A solution, a substrate visualization solution B solution and a stop solution. The invention has the advantages of high detection sensitivity, big accuracy and low cost; the invention can detect the HSP70 protein in a cell lysis solution, a tissue extracting solution, plasma and serum drawn from human, big mice and small mice at the same time.

Cellfree adipose tissue extracts, implants including the same and methods for the preparation thereof. The extracts and implants are capable of inducing adipogenesis and angiogenesis and are, thus, useful in applications including soft tissue repair and engineering and angiogenesis induction, e.g., in wound healing, treatment of burn injuries and ischemic conditions.

The present invention features use of evanescent field sensing to detect or evaluate unpurified, unlabeled analytes in raw samples. Capture molecules capable of binding to an analyte of interest can be immobilized to a sensing surface of an evanescent field sensor. The interaction between the analyte of interest in a raw sample and the immobilized capture molecules alters the refractive index at the sensing surface, thereby producing a detectable signal in the reflected light from the surface. Raw samples amenable to the present invention include, but are not limited to, cell lysates, tissue extracts, bodily fluid, or biologically-derived samples. Evanescent field sensors suitable for the present invention include, but are not limited to, grating-coupled waveguides.

A therapeutic agent for fibromyalgia containing an extract from inflamed tissue inoculated with vaccinia virus as an active ingredient, use of the extract from inflamed tissue inoculated with vaccinia virus as the active ingredient for producing a medicinal composition for treating fibromyalgia, and a method of treating fibromyalgia which comprises administering a medicinal composition containing the extract from inflamed tissue inoculated with vaccinia virus as the active ingredient to a patient. The therapeutic agent containing the extract from inflamed tissue inoculated with vaccinia virus as the active ingredient is a novel therapeutic agent for fibromyalgia, no efficacious therapeutic agent for which has been known heretofore. Moreover, it is very useful as an effective drug having high safety with scarcely any side effects.

The present invention for the first time demonstrated that:(1) bone marrow-derived pluripotent tissue stem cells can be induced in peripheral blood by intravenously administering tissue extract prepared from isolated skin pieces;(2) the substance in the isolated skin pieces, which is responsible for mobilizing bone marrow-derived pluripotent tissue stem cells to peripheral blood, is HMGB1; and(3) HMGB1 with the activity of mobilizing bone marrow-derived pluripotent stem cells to peripheral blood can be easily purified from cultured cells.

The present disclosure relates to a method for treating severe burns using a preparation of tissue-derived material, which may comprise stem cells. The method may include systemic administration of stem cells. The method may also include administration of topical preparations of stem cells or tissue extract.

Methods and devices are disclosed for processing stromal precursor cells (i.e., cells which can differentiate into connective tissue cells, such as in muscles, ligaments, or tendons) which can be obtained from fatty tissue extracts obtained via liposuction. Normal processing of a liposuction extract involves centrifugation, to concentrate the stromal cells into a semi-concentrated form called “spun fat”. That “spun fat” can then be treated by mechanical processing (such as pressure-driven extrusion through 0.5 mm holes) under conditions which can gently pry the stromal cells away from extra-cellular collagen fibers and other debris in the “spun fat”. The extruded mixture is then centrifuged again, to separate a highly-enriched population of stromal cells which is suited for injection back into the patient (along with platelet cells, if desired, to further promote tissue repair or regeneration).

A therapeutic agent for fibromyalgia containing an extract from inflamed tissue inoculated with vaccinia virus as an active ingredient, use of the extract from inflamed tissue inoculated with vaccinia virus as the active ingredient for producing a medicinal composition for treating fibromyalgia, and a method of treating fibromyalgia which comprises administering a medicinal composition containing the extract from inflamed tissue inoculated with vaccinia virus as the active ingredient to a patient. The therapeutic agent containing the extract from inflamed tissue inoculated with vaccinia virus as the active ingredient is a novel therapeutic agent for fibromyalgia, no efficacious therapeutic agent for which has been known heretofore. Moreover, it is very useful as an effective drug having high safety with scarcely any side effects.

The invention provides an additive and a feed capable of improving stress response resistance of prawns. The additive capable of improving the stress response resistance of prawns is mainly prepared from the following components in parts by weight: 2-71 parts of composite vitamins, 0.5-20 parts of amino acids, 0.5-20 parts of taurine, 0.5-20 parts of cupric glycinate, 1-30 parts of zinc methionine, 10-300 parts of composite microbes, 50-500 parts of butyric acid derivatives, 10-100 parts of a horseradish tree extract and 20-400 parts of a yeast tissue / extract. The additive disclosed by the invention can significantly improve the stress response resistance of the prawns and particularly improve the tolerance of the prawns to external stimulus of temperature, salinity, being away from water, high PH and the like.

The present invention for the first time demonstrated that:(1) bone marrow-derived pluripotent tissue stem cells can be induced in peripheral blood by intravenously administering tissue extract prepared from isolated skin pieces;(2) the substance in the isolated skin pieces, which is responsible for mobilizing bone marrow-derived pluripotent tissue stem cells to peripheral blood, is HMGB1; and(3) HMGB1 with the activity of mobilizing bone marrow-derived pluripotent stem cells to peripheral blood can be easily purified from cultured cells.

The invention relates to an attachment base processing method for improving abnormal attachment of stichoposide seeds, which is characterized by comprising the following steps: selecting a polyethylene corrugated plate as an attachment base; then, processing the attachment base by a biological film containing diatom, or soaking the attachment base in the adult tissue extracting solution; and finally, cultivating stichoposide seeds. Based on the feasibility of actual seed production of stichoposide, the invention can obviously improve the abnormal attachment rate of pelagic larvae and the rateof emergence by selecting the proper attachment base and simply processing the attachment base under the condition that the production cost and the manual work are not increased greatly.

Antibodies are described, which are suitable for identification of γ-carboxyglutamic acid (Gla), said antibodies having an ability of identifying Gla residues in proteins and / or peptides, while not reacting with glutamic (Glu) residues in corresponding proteins and / or peptides. Methods for the preparation and identification of said antibodies as well as methods for detection of Gla in biological fluids, tissue extracts, tissue specimens, in which methods said antibodies are used, are also described. There is also described the use of said antibodies for immunopurification of Gla-containing proteins or peptides by, for instance, immunoprecipitation or immunoaffinity chromatography.

Transcription factor is one important protein for gene regulation, is center of gene expression regulating passage and network, is important target of functional genome and protein block research and important target site of transcription treatment and medicine research. The present invention proposes exonuclease III digesting double stranded nucleic acid molecule coated in microporous plate and containing special marker to detect transcription factor protein. The process of analyzing transcription factor expression and activation level includes the following steps: preparing nucleic acid; fixing the nucleic acid molecules to the pores of the microporous plate; incubation of cell or tissue extract containing transcription factor in the plate; incubation of exonuclease III reaction liquid in the plate; incubation of specific conjugate with special marker on nucleic acid on the plate; detecting and analyzing the specific conjugate to obtain the expression and activation level.

A bovine adipocyte-specific polypeptide, termed leptin, is expressed in the fat tissue of cattle. Expression may be altered in over fat cattle, or expression may be in the form of a protein of lesser biological activity relative to that of leaner cattle. The bovine adipocyte polypeptide, DNA and RNA molecules coding therefor, methods for its preparation, and antibodies specific for the polypeptide are disclosed. Methods for determining the susceptibility of cattle to fat deposition are based on measuring the levels of the bovine adipocyte polypeptide in a biological fluid or tissue extract or by measuring mRNA encoding the bovine adipocyte polypeptide in cells of the subject. Methods of evaluating an agent related to the deposition of fat in cattle comprise contacting the agent with an adipocyte in vitro and measuring the amount of the bovine adipocyte polypeptide or mRNA that is produced by the adipocyte. Methods of limiting fat deposition include administering leptin or leptin DNA, and methods of altering intake include administering leptin, leptin DNA, or an antibody directed against leptin.

The present invention provides biomatrix scaffolds, a tissue extract enriched for extracellular matrix components and bound growth factors, cytokines and hormones, and methods of making and using same.

The present invention relates to a computer-implemented method and apparatus for data processing for the purpose of blind separation of nonnegative correlated pure components from smaller number of nonlinear mixtures of mass spectra. More specific, the invention relates to preprocessing of recorded matrix of mixtures spectra by robust principal component analysis, trimmed thresholding, hard thresholding and soft thresholding; empirical kernel map-based nonlinear mappings of preprocessed matrix of mixtures mass spectra into reproducible kernel Hilbert space and linear sparseness and nonnegativity constrained factorization of mapped matrices therein. Thereby, preprocessing of recorded matrix of mixtures mass spectra is performed to suppress higher order monomials of the pure components that are induced by nonlinear mixtures. Components separated by each factorization are correlated with the ones stored in the library. Thereby, component from the library is associated with the separated component by which it has the highest correlation coefficient. Value of the correlation coefficient indicates degree of pureness of the separated component. Separated components that are not assigned to the pure components from the library can be considered as candidates for new pure components. Identified pure components can be used for identification of compounds in chemical synthesis, food quality inspection or pollution inspection, identification and characterization of compounds obtained from natural sources (microorganisms, plants and animals), or in instrumental diagnostics—determination and identification of metabolites and biomarkers present in biological fluids (urine, blood plasma, cerebrospinal fluid, saliva, amniotic fluid, bile, tears, etc.) or tissue extracts.

The present invention is directed to novel methods comprising the isolation of viable fetal progenitor cells from organs of aborted fetus. The invention comprises the use of natural proteolytic, collagenolytic and fibrinolytic activity of autologous abortive placenta tissue extract.

The invention provides a wet tissue container of small distortion and high air tightness. The wet tissue container (100) of the invention used for containing wet tissues (P) is provided with a container (1) with a wet tissue extract opening on the upper part, a bottom cap (2) with an opening (11) on the base for closing the container, a top cap (3) arranged on the container for closing the extract opening (12), an operate part (4) arranged on an operate part install part (18) of the container, an air tightness part (6) for closing the extract opening to let a space part (5) in an air tightness state. The air tightness part is provided with a body-side air tightness part (61) arranged on the container and a cap-side air tightness part (62) arranged on the top cap. The containing space part is made into air tightness state by meshing the body-side air tightness part with the cap-side air tightness part. The shape of the top cap and other parts of the container besides the operate part and install part are formed as curved surface.

A diagnostic test and method is provided comprising mixing blood or another biological fluid sample with a test compound and spotting the blood on filter paper for subsequent analysis of the effect of the test compound on the blood. The biological fluid can be a cerebrospinal fluid, a peritoneal fluid, a cyst fluid, an amniotic fluid, a lavage fluid, a saliva, a cell extract or a tissue extract. The compound is chosen among an amino acid, a peptide, a protein, a carbohydrate, an oligosaccharide, a polysaccharide, a glycoprotein, a lipid, a lipoprotein, a glycosaminoglycan, a hormone, a steroid, a vitamin, a low molecular weight synthetic or natural compound which influences the blood to cause an alteration of its composition, e.g., a toxin, allergen, autoantigen, bacterial protein or polysaccharide, viral protein, fungal protein or polysaccharide, parasitic protein or polysaccharide, bacterial lipopolysaccharide or any other compound relevant to diseases.

The invention provides a process for preparing capped mRNAs from an RNA mixture, e.g. whole RNA isolated from a cell or tissue extract, that includes combining in a reaction mixture RNA comprising capped mRNA with a separable affinity matrix having high-affinity eIF4E bound thereto, under conditions sufficient for binding to occur between the high-affinity eIF4E and the capped mRNA, whereby capped mRNA is bound to the affinity matrix, separating the affinity matrix from the reaction mixture, then separating the capped mRNA from the affinity matrix. High affinity eIF4E mutants previously described are employed in the process as well as a novel mutant disclosed and claimed herein. The mRNA preparation process is based on isolation of 5′-capped mRNA. The mRNA molecules thus isolated have intact sequences encoding the NH2-terminal ends of the proteins they encode, unlike those isolated by prior methods. In addition, use of the method isolates mRNA sequences not isolatable by prior methods that relied on binding to polyadenylated 3′-end sequences.

The present invention relates to biological technology, and is especially process of purifying erythromycin with erythromycin molecular engram polymer. The process includes the following steps: 1. dissolving the mixture of template molecule erythromycin, functional monomer methacrylic acid and cross-linking agent ethylene glycol dimethacrylate in pore creating agent, adding initiator and sealing; 2. grinding the synthesized polymer, eliminating fine grains and extracting to eliminate template molecule; 3. drying the polymer with the template molecule eliminated at 60 for a night to obtain erythromycin molecular engram polymer; 4. filling the erythromycin molecular engram polymer into solid extracting column tube; and 5. activating the solid extracting column, adding animal tissue extracting liquid or water sample dissolving in 40 % concentration methanol solution, and eluting the solid extracting column to elute and purify erythromycin.

The invention relates to a paper towel extracting device, which comprises a casing with a paper towel outlet, a rotating shaft, a motor, a torque sensor and a controller arranged inside the casing, and a pressing mechanism arranged at the paper towel outlet; the rotating shaft and the torque The input end of the sensor is in contact, and the torque sensor senses the torque brought by the rotating shaft to obtain a trigger signal and transmit the trigger signal to the controller. The controller controls the motor to rotate and output paper towels according to the trigger signal, and controls the pressing mechanism to compress the paper towels. The paper towel extracting device of the present invention triggers the extraction of paper towels through a torque sensor, and sets a preset paper towel extension length to avoid excessive waste of paper towels. After the paper towels stop outputting, it will automatically compress the paper towels so that the user can tear away the paper towels , Integrating the sending and stopping of paper towels into the action of people taking away paper towels, no other redundant operations are required, and the problem of cross-infection caused by multiple people touching the paper pumping machine is avoided.

The invention belongs to the field of biotechnologies and relates to a method for preserving rubber tree leaf tissues extracted by DNA (deoxyribonucleic acid). The method comprises the following steps: placing collected rubber tree leaves in a pre-cooled mortar, and utilizing liquid nitrogen for grinding until the leaves become powder; and then transferring the powdery leaf tissues into a centrifugal tube containing storage buffer solution, uniformly mixing and placing in a low-temperature environment for preservation. The method which combines the storage buffer solution with low-temperaturerefrigeration is utilized for preserving the rubber tree leaf tissues, thereby effectively realizing the freshness of the rubber tree leaves for a long time, facilitating long-distance sampling, material transportation and storage, and having the characteristics of simplicity in process, low cost, good effects and the like.

The present invention relates methods for the biosynthesis of tetrodotoxin (TTX) involving the steps of obtaining a culture possessing one or more of a Vibrio species, such as through a seed culture / inoculating the culture and tissue extract from a textrodotoxin-bearing organism in a fermenter medium, and isolating and purifying tetrodotoxin from said fermenter, resulting in a yield of TTX of about 0.5 g TTX / L in about 3 to 5 days, such TTX being at least 90% pure.