16 results about "Tissue extracts" patented technology

The present invention relates to an extract of in vitro cultured C. sativa root tissues, characterized in that said extract comprises at least one lignanamide. The present invention also relates to a pharmaceutical, dermatological, nutraceutical, or cosmetic composition comprising, as an active ingredient, the extract described above.

The invention belongs to the technical field of cosmetics, and discloses a saussurea involucrate callus extract as well as a preparation method and application thereof. The preparation method of the saussurea involucrata callus extract comprises the following steps: inoculating saussurea involucrata into an induction culture medium for culturing to obtain saussurea involucrata callus; inoculating the saussurea involucrata callus into a growth culture medium for culturing, and centrifuging to obtain saussurea involucrata suspension cells; and mixing the saussurea involucrata suspension cells with an extraction solvent, and performing high-pressure homogenization to obtain the saussurea involucrata callus extract. According to the preparation method disclosed by the invention, callus culture, high-pressure homogenization and the deep-eutectic solvent are combined, the efficiency of obtaining active ingredients in the saussurea involucrata callus is improved through a green process, and the saussurea involucrata callus extract with a remarkable anti-inflammatory effect is prepared.

The invention relates to an active pharmaceutical ingredient for repairing oral mucosa and a preparation method of the active pharmaceutical ingredient, and belongs to the technical field of biological medicine, and the active pharmaceutical ingredient comprises an amanita aurantiaca mycelium active peptide-myrobalan polyphenol composite entrapment substance, a dendrobium nobile callus extract, sorbitol, menthol, a gel matrix and the like. The amanita aurantiaca mycelium active peptide-myrobalan polyphenol composite entrapment substance is obtained by entrapment of a compound of amanita aurantiaca mycelium active peptide and myrobalan polyphenol through a sodium alginate-dopamine grafted copolymer; the amanita aurantiaca mycelium active peptide is obtained by performing common enzymolysis on the mycelium of amanita aurantiaca through bromelain and bacillus subtilis protease. The dendrobium nobile callus extract is obtained by inducing slices of dendrobium nobile seedling leaves with 2, 4-D and 6-BA, extracting and purifying. The gel matrix is prepared from modified konjac glucomannan and carboxymethyl chitosan. All the components form an anti-inflammatory, proliferative and remodeled closed-loop repair system, and the anti-degradation stability and the adhesion long-term effect are improved.

The application discloses a purification method of plant exosome, and belongs to the technical field of biological separation. The method comprises the following steps: obtaining plant juice or tissue extract, pre-treating the plant juice or tissue extract through coarse filtration and centrifugation, incubating the plant juice or tissue extract with magnetic microspheres modified with carboxyl or epoxy groups in a buffer containing calcium ions, capturing the complex by using an external magnetic field, washing the complex, eluting the complex by using a competitive elution buffer containing N-acetylglucosamine, and further purifying the complex by using a size exclusion chromatography column to obtain the plant exosome. The exosome is specifically captured by the magnetic microspheres, and the double purification strategies of competitive elution and size exclusion chromatography are combined, so that the recovery rate and purity of the exosome are significantly improved. The method is simple in operation, short in time consumption, good in repeatability, and suitable for the large-scale preparation of exosomes of various plant sources. The plant-derived exosome prepared by the method can be applied to the fields of drug delivery systems, cosmetics, respiratory health and sensory health product development.

The invention relates to the field of plant biotechnology and daily chemical products, and particularly discloses a culture method of rhizoma nardostachyos calluses and application of the rhizoma nardostachyos calluses in preparation of hair care products. The rhizoma nardostachyos callus extract obtained through the culture method has the effects of promoting hair growth, preventing alopecia and regulating scalp grease secretion, and the effect of the rhizoma nardostachyos callus extract is remarkably superior to that of a traditional rhizoma nardostachyos medicinal material extract. The rhizoma nardostachyos callus extract can be used as a novel hair care raw material for replacing a traditional rhizoma nardostachyos medicinal material, and has the characteristics of sustainability and environmental protection.

The present application relates to a kind of active pharmaceutical ingredients of repairing oral mucosa and preparation method thereof, belong to biological medicine technical field, and drug includes orange cover gonggao mycelium active peptide- Terminalia polyphenol composite package, dendrobium nobile callus extract, sorbitol, menthol, gel matrix etc.Orange cover gonggao mycelium active peptide- Terminalia polyphenol composite package is the complex of orange cover gonggao mycelium active peptide and Terminalia polyphenol by sodium alginate-dopamine graft copolymer package;Orange cover gonggao mycelium active peptide is the mycelium of orange cover gonggao mushroom by bromelain and bacillus subtilis protease common enzymolysis obtains.Golden lace dendrobium callus extract is the section of golden lace dendrobium seedling leaf by 2,4-D and 6-BA induction, extraction, purification and obtains.Gel matrix is prepared by modified konjac glucomannan and carboxymethyl chitosan.Each component forms anti-inflammatory, proliferation and remodeling closed-loop repair system, and improves the stability of anti-degradation and adheres long-acting.

The application belongs to the technical field of cosmetics, and discloses a composition with instant soothing effect, a preparation method and application thereof. The composition comprises components: hydroxyphenylpropionamide benzoic acid and / or mother chamomile flower oil, farnesol and / or hydroxyl asperosaponin, kava pepper leaf / root / stem extract and / or menthol lactate, and edelweiss callus extract. The composition has significant soothing effect and can instantly soothe, thereby instantly relieving the uncomfortable feeling such as stinging, burning, swelling and itching, through reasonable compounding of hydroxyphenylpropionamide benzoic acid and / or mother chamomile flower oil, farnesol and / or hydroxyl asperosaponin, kava pepper leaf / root / stem extract and / or menthol lactate, and edelweiss callus extract.

The invention belongs to the field of electrochemical sensors, and provides a construction method and application of an electrochemical sensor for detecting the pH of brain glioma, and the specific process is as follows: firstly, a bimetallic MOF (Zr-TCPP (Fe)) with a heme-like structure (containing Fe element and porphyrin ring) is synthesized, and the material has electrochemical response to pH; 12 sulfydryl dodecyl phosphoric acid (12MA) is fixed on the surface of the gold electrode through an Au-S bond. Furthermore, the MOF material is immobilized on the surface of the electrode by using a Zr-O-P bond between 12MA and Zr-TCPP (Fe). The method can be applied to pH detection of the brain glioma tissue extracting solution.

The invention relates to the field of cosmetics, in particular to an astragalus membranaceus callus extract, a preparation method thereof and cosmetics. The method for preparing the astragalus membranaceus callus extract comprises the following steps: culturing under a long-day condition to obtain astragalus membranaceus calluses; the method comprises the following steps: extracting astragalus membranaceus calluses by taking propylene glycol as a solvent; wherein the astragalus membranaceus callus is obtained by inoculating the astragalus membranaceus callus to an induction culture medium for induction, and the induction culture medium comprises 6-benayl aminopurine, naphthylacetic acid and 2, 4-dichlorphenoxyacetic acid; based on the total volume of the induction culture medium, the concentration of the 6-benayl aminopurine is 0.5-2.0 mg / L; the concentration of the naphthylacetic acid is 0.5-2.0 mg / L; the concentration of the 2, 4-dichlorphenoxyacetic acid is 0.1 mg / L to 1.0 mg / L. According to the technical scheme, the content and quality of active ingredients are remarkably improved. The obtained extract has relatively strong antioxidant and whitening activity, is free of other organic solvent residues, is clear in color, and has a relatively good prospect as a cosmetic raw material.

This invention discloses a soothing, penetration-promoting, and moisturizing lip composition, its preparation method, and its application, belonging to the field of cosmetic technology. The composition, by weight, comprises 50.0-80.0 parts of base oil, 1.0-5.0 parts of ginger root oil, 0.2-1.0 parts of *Edelweiss* callus extract, 0.1-1.5 parts of bisabolol, 2.0-5.0 parts of polyglycerol-3 diisostearate, and 0.5-5.0 parts of hydrogenated lecithin. The components synergistically enhance each other, achieving excellent comprehensive performance in transdermal penetration promotion, moisturizing, and soothing. The composition is non-irritating to the lip mucosa, contains no traditional preservatives, and has good safety performance. The preparation process of this composition is simple, suitable for industrial production, and can be used to prepare various lip cosmetics.

The invention relates to the technical field of daily chemicals, and discloses a skin anti-aging composition as well as a preparation method and application thereof. The invention provides a skin anti-aging composition and a preparation method thereof. Comprising the following raw materials in parts by weight: 1 to 20 parts of hydroxypropyl tetrahydropyrantriol, 1 to 10 parts of vaccinium myrtillus fruit extract, 0.1 to 5 parts of tetrahydromethylpyrimidine carboxylic acid, 0.5 to 1.5 parts of paeonia lactiflora root extract, 0.01 to 5 parts of leontopodium alpinum callus extract, 0.01 to 5 parts of crithmum maritimum callus culture filtrate, 0.001 to 4 parts of ergothioneine and 0.1 to 20 parts of animal umbilical cord extract. According to the composition, various extracts and active ingredients are compounded, so that the composition has a synergistic effect, can directly reach the corium layer of skin, improves cell energy metabolism, improves mitochondrial functions, effectively removes free radicals of mitochondria, improves collagen degradation, and improves skin aging through a dual-channel of mitochondrial activation and extracellular matrix repair.

This invention belongs to the field of cosmetic technology and discloses an extract of *Saussurea involucrata* callus tissue, its preparation method, and its application. The preparation steps of the *Saussurea involucrata* callus tissue extract include: inoculating *Saussurea involucrata* in an induction culture medium to obtain *Saussurea involucrata* callus tissue; inoculating the *Saussurea involucrata* callus tissue in a growth culture medium, centrifuging to obtain *Saussurea involucrata* suspension cells; mixing the *Saussurea involucrata* suspension cells with an extraction solvent, homogenizing under high pressure to obtain the *Saussurea involucrata* callus tissue extract. This invention combines callus tissue culture, high-pressure homogenization, and a eutectic solvent, improving the efficiency of obtaining active ingredients from *Saussurea involucrata* callus tissue through a green process, and preparing a *Saussurea involucrata* callus tissue extract with significant anti-inflammatory effects.

The invention discloses a freeze-dried reagent ball special for streptococcus agalactiae CRISPR detection aiming at a tilapia mossambica tissue matrix and application of the freeze-dried reagent ball, and belongs to the field of molecular diagnosis technology and on-site rapid detection of aquatic diseases. The freeze-drying reagent ball is a three-layer spherical freeze-drying body comprising an inner core layer, an isolating layer and a shell layer, the inner core layer contains an RAA amplification component aiming at a streptococcus agalactiae cfb gene, the shell layer contains a CRISPR-Cas12a detection component and a special anti-inhibition buffer system, and the isolating layer is a solid freeze-drying material layer of which the dissolution rate is slower than that of the shell layer. Through the dissolution time sequence control of the three-layer structure, the automatic sequence that an outer shell layer firstly dissolves and neutralizes an inhibitor and presets a detection system and an inner core layer then dissolves and starts amplification is realized, and multi-step reaction is integrated into one-step closed tube operation. During detection, only tilapia tissue crude extract needs to be added, and a result is interpreted through fluorescence after constant-temperature incubation. The method effectively overcomes the problems of aquatic product tissue matrix inhibition, unstable operation of an existing single-tube method and on-site pollution risk, and is high in sensitivity, extremely simple to operate and stable at normal temperature.

The invention belongs to the technical field of skin care products, and particularly relates to a wrinkle-removing moisturizing skin care composition as well as a preparation method and application thereof. The composition is prepared from the following raw materials in percentage by mass: 0.25 to 0.3 percent of dipeptide diaminobutyrylbenzyl amide diacetate, 0.5 to 0.8 percent of oligopeptide-6, 10 to 20 percent of leontopodium alpinum callus extract, 60 to 80 percent of glycerol and the balance of water. In the culture process of the leontopodium alpinum callus, nomilin, cinnamaldehyde and other components are innovatively added into an induced differentiation culture medium. Test results show that the content of the total flavonoids in the leontopodium alpinum callus can be effectively increased through the synergistic effect of the nomilin and the cinnamaldehyde, and the content of the total flavonoids in the leontopodium alpinum callus extract reaches 26.7% or above. The content of total flavonoids in the leontopodium alpinum callus extract is increased, so that the moisturizing and wrinkle-removing effects of the skin care product are remarkably improved.

The invention discloses a day and night separate care product for relieving atopic dermatitis. The day and night separate care product comprises a daily emulsion and a night face cream which are matched for use, the daily emulsion comprises the following components in percentage by weight: 0.2-5% of an anti-irritation component and 0.2-5% of a first micro-ecological component, the night face cream is prepared from the following components in percentage by weight: 0.02 to 3 percent of itching relieving component and 0.02 to 3 percent of second micro-ecological component; wherein the anti-irritation component comprises panthenol, tetrahydromethylpyrimidine carboxylic acid, leontopodium alpinum callus extract and wild soybean seed extract; the first micro-ecological component comprises lactobacillus / soybean milk fermentation product filtrate, a chlorella extract, a lactobacillus fermentation product and beta-glucan; the itching relieving component is prepared from hydrolyzed sericin, hydroxyphenyl propionamide benzoic acid and ascorbyl palmitate; the second micro-ecological component comprises a chlorella extract and a lactobacillus fermentation product. The day and night separate protection product provided by the invention has the characteristics of synergistic interaction, multi-target generalized barrier repair, accurate time sequence and compliance with AD circadian rhythm.

The invention relates to a sensitive skin anti-aging composition containing arenaria kansuensis callus extract as well as a preparation method and application of the sensitive skin anti-aging composition, and belongs to the technical field of cosmetics. The sensitive skin anti-aging composition comprises an arenaria kansuensis callus extract, phytosphingosine, a selaginella tamariscina extract and a hydrolyzed gardenia extract. According to the preparation method, the selaginella tamariscina extract is prepared by adopting a supramolecular extraction technology, so that the active substances of the selaginella tamariscina extract are mildly and efficiently enriched, and the biocompatibility and the transdermal penetration capacity of the extract are remarkably improved. When the arenaria kansuensis callus extract is compounded with the phytosphingosine, the selaginella tamariscina extract and the hydrolyzed gardenia extract, a synergistic effect can be generated, and systematic intervention is performed on a complex skin aging network from four aspects of energy metabolism, barrier repair, cell protection and inflammation regulation; the invention provides a complete technical scheme with mild characteristic and multi-dimensional anti-aging effect for people with sensitive skin.