Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Biomatrix Scaffolds

a biomatrix and scaffold technology, applied in the field of biomatrix scaffolds, can solve the problems of major loss of some matrix components, inability to recapitulate the complex matrix chemistry and architecture of tissue, etc., and achieve the effects of good attachment, improved quality of cryopreserved cells, and higher levels of p450s

Active Publication Date: 2013-08-01
THE UNIV OF NORTH CAROLINA AT CHAPEL HILL
View PDF3 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The present invention introduces a new kind of scaffold made from biomatrix that can better maintain matured cells and lineage-restricted stem cells for an extended period of time. This scaffold also reduces the time needed to produce mature cells. The scaffold is made using specific techniques that retain native collagens that provide signals for lineage restriction and differentiation. This scaffold can also produce sufficient amounts of viruses and pathogens for experimental and therapeutic use.

Problems solved by technology

However, individual matrix components, alone or in combination, are unable to recapitulate a tissue's complex matrix chemistry and architecture.
However, current decellularization protocols result in major losses of some of the matrix components due to the use of matrix-degrading enzymes or buffers that solubilize matrix components.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Biomatrix Scaffolds
  • Biomatrix Scaffolds
  • Biomatrix Scaffolds

Examples

Experimental program
Comparison scheme
Effect test

example 1

REFERENCES FOR EXAMPLE 1

[0187]1. Lanza, R. et al. Handbook of Stem Cells, Vol. 2 volumes. (Elsevier Academic Press, New York City; 2004).[0188]2. Vacanti, J. P. & Langer, R. Tissue engineering: the design and fabrication of living replacement devices for surgical reconstruction and transplantation. Lancet 354 Suppl 1, SI32-34 (1999).[0189]3. * Schmelzer, E. et al. Human hepatic stem cells from fetal and postnatal donors. Journal of Experimental Medicine 204, 1973-1987 [*co-equal first authors; **co-equal senior authors] (2007).[0190]4. Zhang, L., Theise, N., Chua, M. & Reid, L. M. Human hepatic stem cells and hepatoblasts: Symmetry between Liver Development and Liver Regeneration. Hepatology 48, 1598-1607 (2008).[0191]5. Kubota, H. & Reid, L. M. Clonogenic hepatoblasts, common precursors for hepatocytic and biliary lineages, are lacking classical major histocompatibility complex class I antigens. Proceedings of the National Academy of Sciences of the United States of America 97, 121...

example 2

Use of Biomatrix Scaffolds for Cultures of Tumor Cell Lines or Primary Cultures of Tumors

[0239]The biomatrix scaffolds of this invention can be used for producing cultures of tumor cell lines or of primary cultures of tumors. The ability to do this means that a patient's tumor can be assessed for sensitivity to various therapies in an ex vivo assay.

[0240]The biomatrix scaffolds of this invention can also be used as substrata for grafts of tumors (whether syngeneic, allogeneic, or xenogenic) transplanted into hosts.

[0241]The biomatrix scaffolds of this invention can also be used to assess the metastatic potential of a tumor. Tumor cells are seeded at low cell densities onto substrata of biomatrix scaffolds from various tissues. The tumor cells will attach and survive on many types of biomatrix scaffolds. They will grow and form colonies preferentially on some of them. Their ability to form colonies on a specific type of biomatrix scaffold is predictive of the tumor cells' ability to ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
osmolalityaaaaaaaaaa
timeaaaaaaaaaa
timeaaaaaaaaaa
Login to View More

Abstract

The present invention provides biomatrix scaffolds, a tissue extract enriched for extracellular matrix components and bound growth factors, cytokines and hormones, and methods of making and using same.

Description

STATEMENT OF PRIORITY[0001]This application claims the benefit, under 35 U.S.C. §119(e), of U.S. Provisional Application Ser. No. 61 / 360,939, filed Jul. 2, 2010, the entire contents of which are incorporated by reference herein.STATEMENT OF GOVERNMENT SUPPORT[0002]Aspects of this invention were made with government support under National Institutes of Health (NIH) Grant Nos. AA014243 and IP30-DK065933, National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK) Grant No. DK34987, National Cancer Institute (NCI) Grant No. CA016086 and National Institute of Dental and Craniofacial Research No. DE019569. The United States Government has certain rights to this invention.FIELD OF THE INVENTION[0003]This invention concerns biomatrix scaffolds and methods of producing biomatrix scaffolds and their use in diverse applications as intact scaffolds or as scaffolds that are sectioned or pulverized and dispersed in various ways for specific experimental and clinical uses.BACKGROUND ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(United States)
IPC IPC(8): C12N5/00
CPCA61L27/3604A61L27/3683C12N5/0671C12N5/0068C12N2533/90A61L27/3691C12N5/067C12N5/0693C12N7/00C12N2770/24223C12N2770/24252C12N2730/10123C12N2730/10152C12N2710/20023C12N2710/20052A61L2430/28A61L27/24C12N2500/25C12N2500/36C12N2501/20C12N2501/998C12N2506/14C12N2533/54
Inventor WANG, YUNFANGRIED, LOLA CYNTHIA MCADAMSYAMAUCHI, MITSUOCUI, CAI-BINWANG, ANDREW ZHUANGWERNER, MICHAEL EDWARD
Owner THE UNIV OF NORTH CAROLINA AT CHAPEL HILL
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com