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Method for purifying erythromycin by using molecular engram polymer

A technology of molecular imprinting and erythromycin, which is applied in separation methods, chemical instruments and methods, preparation of test samples, etc., can solve the problem of no erythromycin molecular imprinting polymer solid-phase extraction column, etc., and achieve high selectivity Good sex, specificity, and sensitivity-enhancing effect

Inactive Publication Date: 2008-03-26
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far there is no report on the practical application of erythromycin molecularly imprinted polymer solid phase extraction cartridges

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] 1. Preparation of erythromycin molecularly imprinted polymer:

[0024] Mix erythromycin (template) 0.5mol, monomer methacrylic acid (MAA), and crosslinking agent ethylene glycol dimethacrylate (EGDMA) according to the molar ratio of 1:2:20, and dissolve to 2.5ml Porogen (acetonitrile: methanol = 3: 2), placed in an ampoule, then added 0.02 g of initiator azobisisobutyronitrile (AIBN), ultrasonicated for 5 minutes, then flushed with nitrogen for 5 minutes, and vacuum sealed. Water bath at 60°C for 24 hours to obtain erythromycin molecularly imprinted polymer. Take out the blocky polymer, grind it with a mortar, sieve it to an average particle size of 30-60 μm, suspend it with acetone and discard the fine particles. After the polymer was dried, the template was removed by Soxhlet extraction with methanol (containing 10% acetic acid) for 72 hours, and the medium was changed every 8 hours. Take 20 ul of the eluate, and after HPLC determines that the elution is complete, d...

Embodiment 2

[0030] 1. Preparation of erythromycin molecularly imprinted polymer:

[0031] Mix 0.5 mol of erythromycin (template), monomer methacrylic acid (MAA), and cross-linking agent ethylene glycol dimethacrylate (EGDMA) according to the molar ratio of 1:4:20, and dissolve until 5ml Pore ​​agent (acetonitrile: methanol = 3: 2), placed in an ampoule, then added 0.02 g of initiator azobisisobutyronitrile (AIBN), ultrasonicated for 5 minutes, then flushed with nitrogen for 5 minutes, vacuum sealed, 60 ℃ water bath for 36 hours to obtain erythromycin molecularly imprinted polymer. Take out the blocky polymer, grind it with a mortar, sieve it to a particle size of 30-60 μm, suspend it with acetone and discard the fine particles. After drying the polymer, use methanol (containing 10% acetic acid) for Soxhlet extraction for 48 hours to remove the template and change the solution every 8 hours. After the elution was complete as determined by HPLC, it was dried at 60° C. for 24 hours for lat...

Embodiment 3

[0038] 1. Preparation of erythromycin molecularly imprinted polymer:

[0039] Mix 0.5 mol of erythromycin (template), monomer methacrylic acid (MAA), and cross-linking agent ethylene glycol dimethacrylate (EGDMA) according to the molar ratio of 1:3:20, and dissolve until 3ml Pore ​​agent (acetonitrile: methanol = 3: 2), placed in an ampoule, then added 0.02 g of initiator azobisisobutyronitrile (AIBN), ultrasonicated for 10 minutes, then flushed with nitrogen for 5 minutes, vacuum sealed, 65 °C water bath for 24 hours to obtain the molecularly imprinted polymer of erythromycin. Take out the blocky polymer, grind it with a mortar, sieve it to a particle size of about 30-60 μm, suspend it with acetone and discard the fine particles. After the polymer was dried, the template was removed by Soxhlet extraction with methanol (containing 10% acetic acid) for 24 hours. After the elution was complete as determined by HPLC, it was dried at 60° C. for 24 hours for later use.

[0040] ...

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Abstract

The present invention relates to biological technology, and is especially process of purifying erythromycin with erythromycin molecular engram polymer. The process includes the following steps: 1. dissolving the mixture of template molecule erythromycin, functional monomer methacrylic acid and cross-linking agent ethylene glycol dimethacrylate in pore creating agent, adding initiator and sealing; 2. grinding the synthesized polymer, eliminating fine grains and extracting to eliminate template molecule; 3. drying the polymer with the template molecule eliminated at 60 for a night to obtain erythromycin molecular engram polymer; 4. filling the erythromycin molecular engram polymer into solid extracting column tube; and 5. activating the solid extracting column, adding animal tissue extracting liquid or water sample dissolving in 40 % concentration methanol solution, and eluting the solid extracting column to elute and purify erythromycin.

Description

technical field [0001] The invention relates to a method in the technical field of biochemical engineering, specifically, a method for purifying erythromycin by using a molecularly imprinted polymer. Background technique [0002] Erythromycin (ERY for short) belongs to macrolide antibiotics and is a widely used drug shared by humans and animals. It can bind to bacterial ribosomes to prevent the synthesis of bacterial proteins, thereby inhibiting bacteria. . May cause residue if used incorrectly. Harm or pollute people, animals and the environment. Long-term consumption of animal foods that exceed the standard of erythromycin will cause damage to the cochlea and vestibular nerves, and even endanger the functions of the liver and kidney. [0003] In order to ensure the health of the people, countries around the world have made strict regulations on the use and residue of erythromycin and formulated corresponding standards. Among them, the European Union has different limits...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/08C07H1/06B01D15/34G01N1/34G01N30/06
Inventor 张大兵武爱波宋素泉
Owner SHANGHAI JIAO TONG UNIV
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