41 results about "Macrolide Antibiotics" patented technology

The invention discloses trichoderma asperellum TS7-1 capable of efficiently degrading macrolide antibiotics and application of the trichoderma asperellum TS7-1, and belongs to the technical field of microorganisms. The preservation number of the trichoderma asperellum TS7-1 is CGMCC (China General Microbiological Culture Collection Center) No. 41372. The Trichoderma asperellum TS7-1 (CGMCC No.41372) provided by the invention has high-efficiency degradation capability on various macrolide antibiotics (erythromycin, roxithromycin and azithromycin), the removal rates of the Trichoderma asperellum TS7-1, roxithromycin and azithromycin in 36h all reach 85% or above, and the Trichoderma asperellum TS7-1 has high-concentration antibiotic tolerance, degradation rate and degradation spectrum obviously superior to those of other strains in the prior art. The trichoderma asperellum TS7-1 is directly separated from a natural soil body which is not polluted by antibiotics, has no animal and plant pathogenicity hidden danger, has no microbial toxicity to degradation products of macrolide antibiotics, avoids secondary pollution, and has excellent environmental safety.

The invention discloses a bacteriostatic composition which comprises a synergist and a bacteriostatic agent, the synergist is ethyl phenylacetate or a derivative thereof, and the bacteriostatic agent is selected from hydrogen peroxide, beta-lactam antibiotics, macrolide antibiotics and aminoglycoside antibiotics. The synergist disclosed by the invention is wide in synergistic effect spectrum range, and has a remarkable synergistic bacteriostatic effect when being combined with hydrogen peroxide, amoxicillin, ampicillin, benzylpenicillin potassium, erythromycin, azithromycin and amikacin; the antibacterial effect is weak when the compound is independently used, but the virulence of staphylococcus aureus can be remarkably reduced, the formation of hemolysin, staphylococcus flavin, enterotoxin and a biofilm can be inhibited, and meanwhile, the selective pressure on bacteria is favorably reduced due to the relatively low antibacterial activity, so that the occurrence of drug resistance is delayed; the method is suitable for various application scenes such as food processing industry.

The present invention relates to the field of analytical chemistry, and discloses a mass spectrum ionization element for macrolide antibiotic detection and a preparation method thereof, the mass spectrum ionization element comprises: a conductive substrate made of stainless steel; the adsorption layer is attached to at least part of the surface of the substrate, the adsorption layer is formed by a boric acid functionalized covalent organic framework, and the boric acid functionalized covalent organic framework is of a polycyclic structure composed of repeated units shown in the formula I in the specification. The mass spectrum ionization element is uniform in coating, high in selectivity on macrolide enriched antibiotics in a complex matrix and high in enrichment capacity, an eluent of the mass spectrum ionization element can be used for detection based on a liquid chromatography method and can also serve as a mass spectrum ion source solid substrate, mass spectrum detection is conducted through electrospray ionization, the detection steps are simple, and the mass spectrum ionization element is suitable for mass spectrum detection. And the detected background noise is low.

According to the scheme, the invention provides the recombinant macrolide enzyme as well as the preparation method and the application thereof, the amino acid sequence of the recombinant macrolide enzyme is as shown in SEQ ID NO.1, and the recombinant macrolide enzyme is derived from an EreC esterase family of enterobacter hormaechei and is obtained through site-specific mutagenesis; the mutation sites are as follows: glutamic acid at the 44th site is mutated into asparagine, tyrosine at the 55th site is mutated into proline, proline at the 76th site is mutated into arginine, phenylalanine at the 153rd site is mutated into alanine, and serine at the 219th site is mutated into glutamic acid. The method is expected to remove macrolide antibiotic pollution, and has good economic benefits and practical values.

The invention belongs to the technical field of agricultural biology, and particularly relates to zearalenone hydrolase ZHRnZ as well as a coding gene, a preparation method and application thereof. The amino acid sequence of the zearalenone hydrolase ZHRnZ provided by the invention is as shown in SEQ ID NO.1, and the nucleotide sequence of the coding gene of the zearalenone hydrolase ZHRnZ is as shown in SEQ ID NO.2. The hydrolase provided by the invention has relatively strong degradation capability on zearalenone, derivatives of zearalenone and macrolide antibiotics, and is high in degradation speed. The method has a huge application prospect in the fields of degradation of feed, food, biomass energy, Chinese herbal medicine mycotoxin and antibiotics.

The invention relates to the field of new biological materials, in particular to a high-packaging-dose antibacterial slow-release biological patch obtained by packaging at least two antibiotics with an acellular matrix material. The acellular matrix material is derived from any two of small intestinal submucosa, peritoneum, dermis, bladder basement membrane and pericardium; the antibiotics comprise hydrophobic antibiotics and hydrophilic antibiotics, and the hydrophobic antibiotics are selected from one or more of Beta-lactam antibiotics, rifamycin and macrolide antibiotics; the hydrophilic antibiotics are tetracycline antibiotics. The coating-free drug-loaded sustained-release system based on a lamination process kills bacteria in a short period, prevents colonization and reproduction of bacteria in a wound / patch, realizes synergistic and continuous release of antibiotics along with a wound healing process, guides tissue regeneration and synchronously realizes degradation, is applied to a high-incidence infection period of a surgical operation, reduces adhesion, and has a good application prospect. The abdominal wall strength is enhanced, and the growth of wound granulation tissues is promoted.

The invention discloses a method for degrading residual antibiotics in macrolide antibiotic fermentation waste. The method comprises the following steps: taking antibiotic mushroom dregs as a degradation raw material, stainless steel beads as a grinding medium, and taking an oxidizing agent and a pro-oxidizing agent as ball milling aids. The mixture is subjected to a grinding reaction in a ball milling tank, treatment of the mushroom dregs is completed, the content of antibiotics in the mushroom dregs obtained after ball milling is reduced to 99% or below, the antibiotic resistance of the antibiotics disappears, and the mushroom dregs can be further used for re-fermentation of erythromycin. The method has the advantages that the process route is short, the reagent dosage is small, the price is low, operation is easy, the whole process is carried out under the solid-phase condition, no solvent is needed, the degradation efficiency is good, and the method has good application and popularization prospects.

According to the scheme, the recombinant macrolide enzyme and the preparation method and application thereof are provided, the amino acid sequence of the recombinant macrolide enzyme is shown as SEQ ID NO.1, and the recombinant macrolide enzyme is obtained by site-directed mutagenesis of an erythromycin esterase family protein sequence derived from enterobacter hormaechei; the mutation sites are as follows: the 44 glutamic acid is mutated into asparagine, the 55 tyrosine is mutated into proline, the 153 proline is mutated into alanine, and the 219 serine is mutated into glutamic acid, so that the specific macrolide lactonase which is efficient, stable, easy to prepare on a large scale and more suitable for environmental requirements is prepared, and macrolide antibiotic pollution is removed; and the method has good economic benefits and practical values.

The invention relates to the technical field of adsorption material preparation, in particular to Ti3C2Tx hollow microspheres and a preparation method and application thereof.The preparation method of the Ti3C2Tx hollow microspheres comprises the steps that S1, LiF is added into an HCl solution, first stirring is conducted till LiF is dissolved, Ti3AlC2 is added, etching, first centrifugation, washing, ultrasonic treatment and second centrifugation are conducted, and single-layer Ti3C2Tx dispersion liquid is obtained; s2, the single-layer Ti < 3 > C < 2 > T < x > dispersion liquid obtained in the step S1 and PS pellets are subjected to second stirring, filtering, drying and calcination, and Ti < 3 > C < 2 > T < x > hollow microspheres are obtained; wherein in S2, the mass ratio of the Ti < 3 > C < 2 > T < x > to the PS pellets is 1: (3-7); wherein in the step S2, the second stirring condition is as follows: the second stirring temperature is 20-30 DEG C, and the second stirring time is 12-48 hours. The Ti3C2Tx hollow microspheres provided by the invention have relatively fast adsorption kinetics and relatively large adsorption capacity for removing tetracycline and macrolide antibiotics, and are high in physical and chemical stability and strong in pollution resistance.

Provided herein are pharmaceutically acceptable compositions containing macrolide antibiotics, in particular azithromycin. In particular, compositions containing azithromycin with low toxicity, especially for administration to felines, are provided herein.

The invention discloses a method for detecting macrolide antibiotics in a water body, which adopts a dispersive solid-phase extraction method and comprises the following steps: S1, synthesizing a material to obtain a metal organic framework material Ce-MOF of cerium; s2, sample collection: collecting river and lake water samples and transporting the river and lake water samples to a laboratory for analysis; s3, sample pretreatment; s4, analyzing the sample, detecting the macrolide antibiotics through a liquid chromatography tandem mass spectrometer, and adopting an ion electrospray positive ion mode ESI < + > and a multi-reaction monitoring MRM mode. Compared with traditional solid-phase extraction, the method has the advantages that the operation process is simplified, the complexity and time cost of sample treatment are reduced, the accuracy and repeatability are improved, the method is more environment-friendly, and the experimental detection cost is reduced.

The application discloses a preparation method and application of a cobalt monatomic doped strontium titanate composite material, and belongs to the field of water treatment. The cobalt doped strontium titanate composite catalytic material is composed of a cobalt metal doped strontium titanate material. A preparation process uses tetrabutyl titanate and a nitrate as reactants, ethylene glycol and water as solvents, and prepares strontium titanate (SrTiO3: STO) through a hydrothermal method. Then, cobalt monatomic atoms are doped into the strontium titanate through cobalt chloride (a cobalt source) by using an electrostatic adsorption method, so as to form cobalt doped strontium titanate (Co-STO). The material can efficiently activate an oxidant peracetic acid and rapidly degrade various antibiotic pollutants, and especially performs well in degrading quinolone, tetracycline and macrolide antibiotics. The catalytic material has the advantages of low cost, renewability and environmental protection, and is very suitable for being applied to a deep treatment process of pharmaceutical wastewater.

The invention discloses a preparation method and application of a cobalt monatomic doped strontium titanate composite material, and belongs to the field of water treatment. The cobalt-doped strontium titanate composite catalytic material disclosed by the invention is formed by compounding a cobalt metal-doped strontium titanate material. According to the preparation process, tetrabutyl titanate and nitrate are used as reactants, ethylene glycol and water are used as solvents, and strontium titanate (SrTiO3: STO) is prepared through a hydrothermal method. Then, doping cobalt single atoms into strontium titanate through cobalt chloride (a cobalt source) by using an electrostatic adsorption method to form cobalt-doped strontium titanate (Co-STO); the material can efficiently activate an oxidizing agent peracetic acid and rapidly degrade various antibiotic pollutants, and especially has excellent performance in the aspect of degrading quinolones, tetracyclines and macrolide antibiotics. The catalytic material has the advantages of low cost, reproducibility, environmental protection and the like, and is very suitable for the advanced treatment process of pharmaceutical wastewater.

The invention relates to a method for analyzing pollutants in hospital wastewater based on liquid chromatography-high resolution mass spectrometry. The method comprises the following steps: S1, enriching pollutants in hospital wastewater through solid-phase extraction of four specific solid-phase extraction columns, and then analyzing and detecting a liquid to be detected through a specific chromatographic column, a specific liquid chromatography and a high-resolution mass spectrometry which are connected in series; the identification of various pollutants (medicines and metabolites thereof) in the hospital wastewater is realized, so that a data basis is provided for subsequent fingerprint analysis. Specifically, the analysis method provided by the invention can identify 34 kinds of substances, including sulfonamide antibiotics, macrolide antibiotics, cephalosporin antibiotics, quinolone antibiotics, beta-lactam antibiotics, aminoglycoside antibiotics and carbamazepine and metabolites thereof.

A new class of dimeric 2-aminoimidazole (2-AI) compounds that potentiate macrolide antibiotics against A. baumannii. A parent dimer lowers the MIC of clarithromycin (CLR) from 32 μg / mL to 1 μg / mL at a concentration of 7.5 μM (3.4 μg / mL), while a structure activity relationship (SAR) study on the dimeric 2-AI scaffold resulted in the identification of several compounds with increased activity. Substitution of fluorine on a central phenyl ring resulted in the most potent activity, with the lead compound, containing a fluorine ortho to each of the 2-AIs, that lowered the CLR MIC to 2 μg / mL against AB5075 at 1.5 μM (0.72 μg / mL), exceeding the activity of both the parent dimer and the lead aryl-2-AI. Furthermore, these dimeric 2-AI analogs exhibit favorably low mammalian cell toxicity.

The present invention relates to a the genetically engineered multidrug-resistant reporter V. cholera strain BLF-1Vc which has been constructed and laboratory tested for rapid and robust screening of natural and synthetic molecules for inhibition of enzymes involved in β- lactams, aminoglycosides and macrolide antibiotic resistance and determine its antibiotic 5 potentiating, synergistic or novel antibacterial property. The reporter strain disclosed herein can also be used for testing novel drugs and antimicrobial agents for its efficacy against multidrug resistant clinical isolates. The present invention also discloses a method for preparing a multidrug-resistant reporter microorganism and a kit for detecting antibiotic resistance or screening antimicrobial compounds, comprising the genetically engineered 0 multidrug-resistant reporter V. cholera strain BLF-1Vc.

The application discloses a synergistic bactericidal composition, comprising the following components: a toona sinensis extract: 16-2048 mu g / mL; a macrolide antibiotic: 0.0625-4096 mu g / mL; the bacteria are staphylococcus aureus or methicillin-resistant staphylococcus aureus, and the macrolide antibiotic is erythromycin or azithromycin. The application further discloses a use of the synergistic bactericidal composition in preparation of a medicine for treating skin and soft tissue infections. The composition provided by the application can convert the traditional macrolide antibiotics such as erythromycin and azithromycin which only have a bacteriostatic effect into a preparation which has clear bactericidal activity on staphylococcus aureus and drug-resistant strains thereof. After the toona sinensis extract is combined with the antibiotic, a synergistic effect is exhibited, and the concentration of the toona sinensis extract and the antibiotic required when the toona sinensis extract and the antibiotic are used alone can be greatly reduced.

This invention discloses a screening and detection method for antibiotic residues in organic fertilizers, relating to the field of trace residue detection. The detection method includes the following steps: S1, mixing the organic fertilizer sample with a buffer solution to obtain an extract; S2, adding a divalent calcium ion compound to the extract, mixing well, allowing it to stand, centrifuging, discarding the supernatant, and collecting the precipitate; S3, adding an alkaline releasing agent to the precipitate to dissolve it, centrifuging, collecting the supernatant, and obtaining a release solution; S4, reacting the release solution with a colorimetric reagent, measuring the visible light absorbance, and calculating the antibiotic content based on a standard curve. This method not only completely avoids the destruction of antibiotics by strong acids but also transforms humic acid from an interfering substance into an enrichment tool, thereby significantly improving the extraction rate. It is simple to operate and low in cost, thus providing a novel solution for the detection of macrolide antibiotic residues in organic fertilizers.

The present invention is a method for regulating the fermentation of macrolide antibiotics. It solves the problem that the fermentation process of the traditional method is not easy to control, the energy consumption is high, and the yield of the target product is low. It includes the following steps: in the primary metabolic stage of macrolide antibiotic producing bacteria, the culture temperature is controlled at 27.0-30.0°C according to normal requirements, and the mycelium grows rapidly; after the macrolide antibiotic producing bacteria complete the logarithmic growth period, the fermentation culture temperature is reduced to 18.0-27.0°C to promote the large-scale synthesis of the target product, and the macrolide antibiotic is an antibiotic and its homologues produced by fermentation using the PKS biosynthetic pathway. The process of the present invention is simple and easy to operate, and is very suitable for commercial production.

The invention discloses a method for measuring the concentration of macrolide antibiotics in domestic sewage. The method comprises the following steps: enriching and purifying macrolide compounds in a treated domestic sewage sample through a non-polar divinyl phenyl neutral polymer solid-phase extraction column and a hydrophilic group-containing polystyrene / divinyl benzene copolymer bonded piperazine group solid-phase extraction column which are connected in series, concentrating, and fixing the volume; and separating the target compound by liquid chromatography, and detecting by a triple quadrupole mass spectrometry detector to obtain the concentration of the macrolide antibiotics in the domestic sewage. The method overcomes the defects of traditional detection, reduces the interference of the matrix effect of the water sample on the internal standard, improves the quantitative accuracy, does not involve expensive materials, can obtain reagent consumables in situ, and saves the detection cost.

The invention relates to a transcription factor-based cell-free biosensor and an application thereof in water environment antibiotic detection. Firstly, a cell-free biosensor containing TetR or MphR is constructed, the cell-free biosensor comprises a DNA template, a transcription factor TetR or MphR, RNA polymerase and a DFHBI-1T fluorescent probe, the DNA template comprises a reporter gene capable of being combined with the fluorescent probe, and an operon corresponding to the transcription factor TetR or MphR is arranged on the upstream of the reporter gene; the biosensor can respond to tetracycline or macrolide antibiotics, and further, the content of the antibiotics can be measured according to the change of fluorescence intensity in a cell-free biosensor system. The invention provides the cell-free biosensor with high sensitivity and high stability, and the biosensor can realize high-efficiency detection under different water body conditions and realize stronger environmental adaptability.

The invention belongs to the technical field of biological detection, genotype detection of a mutation site of Bordetella pertussis 23S rRNA A2047G is realized based on a PCR-melting curve method, and then macrolide antibiotic resistant Bordetella pertussis is detected, and the following technical scheme is adopted: the detection kit for the macrolide antibiotic resistant Bordetella pertussis has the advantages that the genotype of the mutation site of the Bordetella pertussis 23S rRNA A2047G mutation site is detected; the kit comprises a reaction solution, a detection solution, a sensitive positive control, a drug-resistant positive control and a blank control. The detection method comprises the following steps: S1, taking a bordetella pertussis DNA sample as a template, simultaneously using sensitive positive control, drug-resistant positive control and blank control, and using the macrolide antibiotic drug-resistant bordetella pertussis detection kit in the patent for detection; and S2, data processing: after the reaction is finished, performing result judgment through a melting curve of the sample.

This invention discloses a method for degrading residual antibiotics in fermentation waste containing macrolide antibiotics. The method includes using antibiotic fermentation residue as the degradation raw material, stainless steel beads as the grinding medium, and oxidants and co-oxidants as ball milling aids. The mixture is ground and reacted in a ball mill jar to complete the treatment of the fermentation residue. The antibiotic content in the ball-milled residue is reduced to below 99%, with the antibiotics losing their anti-viability, and the residue can be further used for the re-fermentation of erythromycin. This invention features a short process route, low reagent consumption and cost, simple operation, and the entire process is carried out under solid-phase conditions without solvents, resulting in high degradation efficiency. It is a method for treating macrolide antibiotic fermentation residue with good prospects for widespread application.

The present invention refers to an in vitro method for the diagnosis of a Mycoplasma pneumoniae infection and / or for the differential diagnosis between a Mycoplasma pneumoniae pneumonia and other bacterial or viral pneumonias and / or for assessing whether to treat a patient suffering from pneumonia with a macrolide antibiotic.

The invention provides a hybridoma cell strain CLA-4D3 capable of generating an anti-clarithromycin monoclonal antibody and application of the hybridoma cell strain CLA-4D3, and belongs to the technical field of monoclonal antibody preparation. The hybridoma cell strain CLA-4D3 provided by the invention is preserved in the China Center for Type Culture Collection on August 1, 2025, and the preservation number of the hybridoma cell strain CLA-4D3 is CCTCC (China Center for Type Culture Collection) NO: C2025232. The anti-clarithromycin monoclonal antibody is high in titer, the subtype of the anti-clarithromycin monoclonal antibody is IgG1, the semi-inhibition ratio IC50 of the anti-clarithromycin monoclonal antibody to clarithromycin is 1.52 [mu] g / L, the cross reaction rate of the anti-clarithromycin monoclonal antibody to clarithromycin is 100.00%, and the anti-clarithromycin monoclonal antibody has a good cross reaction with structural analogues. The anti-clarithromycin monoclonal antibody generated by the hybridoma cell strain CLA-4D3 disclosed by the invention can realize the purpose of rapidly and sensitively detecting 14-ring macrolide antibiotics such as clarithromycin, erythromycin and roxithromycin in food and environment.

The invention relates to a primer probe group, a kit and a method for detecting macrolide antibiotic resistant bordetella pertussis, and particularly, primers and probes aim at the 2047 site of a 23S rRNA (ribosomal Ribonucleic Acid) gene of bordetella pertussis. The inventor optimizes the reaction conditions (annealing temperature, primer concentration and the like) of the PCR amplification reaction, so that the sensitivity and specificity of the kit and the method are improved, and the result accuracy is ensured.

The invention relates to the technical field of antibiotic detection, and particularly discloses a sample treatment method for detecting macrolide antibiotics in a plant source matrix sample. The sample treatment method provided by the invention comprises the following steps: uniformly mixing a plant source matrix sample with a buffer solution, adding acidified acetonitrile, salting out and absorbing water by using a mixed inorganic salt, and purifying by using an adsorbent comprising anhydrous magnesium sulfate, an aliphatic diamine derivative and O-terminated octadecyl bonded silica gel to obtain the treated plant source matrix sample. Through the systematic design of extraction-separation-purification, the chemical properties of the target object and the characteristics of the plant matrix are combined, and the synergistic effect of the buffer solution, acidified acetonitrile, salting-out and multiple adsorbents is utilized, so that matrix interference can be effectively removed, the target object is stable, and efficient extraction of macrolide antibiotics is realized. The method is suitable for detecting the macrolide antibiotics such as lincomycin, tilmicosin and erythromycin in the plant source matrix.

This invention belongs to the fields of biomedicine and bioengineering technology, specifically relating to mutants of *Streptomyces freundii* TylF methyltransferase and their application in tylosin production. The amino acid sequences of mutants and combined mutants of the TylF methyltransferase amino acid sites, and the corresponding nucleotide sequences of the encoding genes are provided. These mutants significantly improve the enzyme activity characteristics of TylF: (1) the mutants have higher TylF methyltransferase activity, which can significantly increase tylosin yield; (2) the mutants have a lower optimal catalytic temperature (30℃), which allows for efficient conversion of tylosin C to tylosin A (tylosin C / A conversion) without raising the temperature to 38℃ in the later stages of fermentation, reducing production energy consumption. This invention has application value in the genetic engineering modification of macrolide antibiotic biosynthetic strains such as tylosin and in the industrial production of macrolide antibiotics such as tylosin.

The utility model provides a detection strip for macrolide antibiotics. The detection strip comprises a lower shell, an upper shell and a test strip, a display groove and a detection opening are formed in the upper shell; a connecting sleeve is integrally formed in the upper shell; a connecting column is integrally formed at the position, corresponding to the connecting sleeve, in the lower shell, the upper shell and the lower shell are detachably connected through the connecting sleeve and the connecting column, a supporting assembly is arranged in the lower shell, and the test strip is movably arranged in the supporting assembly; a sealing structure is also arranged between the upper shell and the lower shell; the top of the upper shell is provided with a sealing film assembly used for covering the display groove and the detection opening. According to the utility model, the effect that the test strip is completely sealed by the upper shell and the lower shell when the test strip is not used in daily life is effectively achieved; the problems that the detection precision of the test strip is reduced and the test strip fails due to the contact between external humid air, carbon dioxide and suspended microorganisms and the test strip are avoided.