17 results about "Tilmicosin" patented technology

The invention relates to a nucleic acid aptamer for specifically recognizing tilmicosin as well as screening and application of the nucleic acid aptamer, and belongs to the technical field of food safety detection and biosensing. Aiming at the defects of the existing method for detecting tilmicosin residues in food, the invention provides a high-affinity and high-specificity nucleic acid aptamer obtained by screening based on a graphene oxide index enrichment ligand systematic evolution technology. The aptamer is obtained by multiple rounds of screening from a random single-stranded DNA library through a GO-SELEX technology, and a core sequence Apt-2-1 with the length of 33 nt is finally obtained through sequence analysis and structure optimization. The aptamer shows excellent binding capacity to tilmicosin, the dissociation constant reaches 9.82 nM, and the aptamer has good specificity. The nucleic acid aptamer provided by the invention can be used for constructing a rapid and sensitive tilmicosin residue detection method, and has important application value in monitoring of livestock and poultry products and food safety.

This invention belongs to the field of immunology and discloses a hybridoma cell line and the preparation and application of its secreted monoclonal antibody that uniformly recognizes tylosin / tilmicosin. The monoclonal antibody hybridoma cell line is named DES-AOAA-14D5, with accession number CGMCC NO: 45303. This invention utilizes decarboxymethyl tylosin and oxycarboxymethyl hydroxylamine to synthesize a hapten, conjugates a carrier protein to prepare a complete antigen, immunizes mice, and, through cell fusion and screening techniques, prepares a hybridoma cell line that can secrete a monoclonal antibody that uniformly recognizes tylosin / tilmicosin. IC50 50 The effective concentrations were 1.59 and 1.72 ng / mL, respectively, with a cross-reactivity rate as high as 92.44%. An immunoassay method for the simultaneous and accurate detection of tylosin / tilmicosin in milk was established using this antibody. The cut-off value for both tylosin and tilmicosin was 16 ng / mL, and no cross-reactivity was observed with other structural analogs. The detection method of this invention shows promising application prospects.

The utility model relates to the technical field of tilmicosin production, and discloses a transfer kettle for tilmicosin production, which comprises a kettle body, the top end of the kettle body is fixedly connected with a mounting base, the top end of the mounting base is fixedly connected with a driving motor, the output end of the driving motor is fixedly connected with a rotating shaft, and one end of the rotating shaft penetrates through and extends to the inner side of the kettle body. Two stirring plates are fixedly connected to the outer side of the rotating shaft, a plurality of input pipes are communicated to the top end of the kettle body, an observation opening is formed in the top end of the kettle body, a door opening and closing mechanism is arranged at the top end of the observation opening, a discharging pipe is communicated to the bottom end of the kettle body, and a discharging pipe with a valve opening and closing function is communicated to the bottom end of the kettle body; by arranging the driving motor, the rotating shaft, the stirring plates and the observation opening, whether tilmicosin is caked or not can be observed through the observation opening, if the tilmicosin is caked, the driving motor is started, the rotating shaft and the two stirring plates are driven by the driving motor to stir tilmicosin, and the tilmicosin is prevented from being caked after being placed for a long time.

The utility model relates to the technical field of water-soluble powder production, and discloses a tilmicosin powder production device which comprises a treatment tank, a feed hopper part arranged at the top of the treatment tank, a sealing part arranged at the top of the feed hopper part, a pretreatment device arranged inside the feed hopper part and an internal processing device arranged inside the treatment tank, and the inner processing device comprises a rotating shaft rod, a driving motor part, a connecting circular plate, a connecting rod part, an arc plate, a middle connecting rod part, a placing barrel, an inner placing block and a temperature and humidity detection sensor. According to the tilmicosin powder production device, key parameters such as temperature, humidity, pressure and stirring speed in the production process can be monitored in real time by arranging an internal processing device, arranging a placement barrel on the surface of a rotating shaft rod and arranging a temperature and humidity detection sensor in the placement barrel; and the parameters are automatically adjusted according to preset process requirements, so that the stability and consistency of the production process are ensured, and the production quality is ensured.

The invention discloses a tilmicosin nanostructure lipid carrier and a preparation method thereof. The preparation method comprises the following steps: swelling poloxamer 188 in distilled water to serve as a water phase; the preparation method comprises the following steps: mixing and melting stearic acid and oleic acid, sequentially adding tilmicosin and Tween 80, and stirring to form an oil phase; controlling the water phase and the oil phase at the same temperature, adding the oil phase into the water phase, shearing at a high speed, and homogenizing at a high pressure to obtain a pre-emulsion; and mixing the pre-emulsion with water and then carrying out secondary high-speed shearing. The carrier can keep good stability under the acidic condition, can significantly improve the oral bioavailability of tilmicosin in piglet bodies, can promote cell uptake, and can reduce the efflux effect of a drug transporter, thereby effectively improving the efficiency of tilmicosin entering intestinal epithelial cells. In addition, the carrier can well cover the unpleasant odor and bitter taste of the medicine, the palatability of tilmicosin is improved, and the influence of tilmicosin on pig ingestion is avoided.

The invention discloses an enzymatic cascade nanogel system targeting Lawsonia intracellularis and a preparation method of the enzymatic cascade nanogel system. The enzymatic cascade nanogel system comprises the following components: 0.05-2% of sodium alginate; 0.02 to 0.3 percent of calcium chloride; 2 to 6 percent of N, O-carboxymethyl chitosan; 0.2 to 1% of D-mannosamine hydrochloride; 0.05 to 0.2 percent of tilmicosin; 0.0005 to 0.002 percent of cerium dioxide nano enzyme; and 0.0005 to 0.002 percent of glucose oxidase. The enzymatic cascade nanogel system provided by the invention has a dual gel structure of a dynamic covalent bond core and an ionic bond shell, and can realize targeted programmed release of tilmicosin; the endocytosis of host cells on the enzymatic cascade nanogel system is actively enhanced, the intracellular transport capacity of tilmicosin is improved, and the intracellular drug concentration is improved; glucose oxidase consumes glucose to generate gluconic acid and H2O2 so as to realize'hunger therapy 'on Lawsonia intracellularis; h2O2 generated by decomposing glucose oxidase by cerium dioxide nano-enzyme generates high-concentration oxygen at an infected part, so that the living environment of the intracellular Lawsonia is deteriorated.

This utility model discloses a quantitative weighing device for tilmicosin production, including a second conveyor line. A drive mechanism is provided on one side of the second conveyor line. The drive mechanism includes a gantry frame with a mounting bracket that slides vertically up and down on its inner side. A telescopic cylinder is horizontally mounted on the mounting bracket, and a support plate is horizontally fixed to the front end of the telescopic cylinder's telescopic rod. Multiple U-shaped placement slots for placing measuring cylinders are provided on the front side of the support plate. The arrangement direction of the U-shaped placement slots is consistent with the conveying direction of the second conveyor line. The openings of the U-shaped placement slots face the front side of the support plate, and multiple weighing sensors are arranged around the top surface of the support plate in a circle around the U-shaped placement slots. This utility model can automatically transport the measuring cylinders after quantitative feeding and can automatically convey the measuring cylinders, improving work efficiency and saving manpower.

The invention discloses a method for rapidly detecting various veterinary drug residues in a dairy product by adopting a biochip. The method comprises the following steps: (a) recovering a dairy product sample to be detected, a biochip detection card and micropores to room temperature; (b) selecting a biochip detection card and a micropore, marking the biochip detection card and the micropore, and preheating the micropore on a dry heater; (c) adding a to-be-detected sample into the micropores, repeatedly sucking, beating and uniformly mixing until a micropore solid is completely dissolved, and incubating; (d) transferring all the liquid in the micropores into a sample adding hole of the biochip detection card, uniformly mixing and reacting; and (e) interpreting a result. The method for detecting the chloramphenicol, the lincomycin, the tylosin and the tilmicosin in the dairy product is low in detection limit and can meet the dairy product limit requirement formulated in the national standard.

The invention belongs to the technical field of drug solvent residue detection, and particularly relates to a method for detecting a residual solvent 3, 5-dimethylpiperidine in a tilmicosin bulk drug. The method comprises the following steps: preparing a reference solution and a test solution, determining the content of the residual solvent 3, 5-dimethylpiperidine in the tilmicosin bulk drug by adopting gas chromatography-headspace sampling, and calculating the residual quantity according to an external standard method. The detection method is simple, accurate, good in repeatability and high in sensitivity; the method is suitable for daily detection of 3, 5-dimethylpiperidine serving as a tilmicosin raw material medicine, and the medication safety of tilmicosin is ensured by controlling the residual solvent amount in veterinary medicine tilmicosin, so that infectious diseases of animals such as pigs and chickens can be effectively and safely prevented and treated. According to the method, the blank of the method for detecting the residual solvent 3, 5-dimethylpiperidine in the tilmicosin is filled, the quality of the raw material medicine is effectively improved, and the clinical medication risk is reduced.

This application provides a *Staphylococcus pseudoci* AT8 strain for degrading antibiotics and its application. The *Staphylococcus pseudoci* is a species of Staphylococcus (…). Staphylococcus The *Staphylococcus pseudocarpa* species, having a 16S rDNA sequence as shown in SEQ ID NO:1, is named... Staphylococcus pseudoxylosus AT8, deposited on November 20, 2025 at the Guangdong Provincial Center for Microbial Culture Collection, accession number: GDMCC No: 67323. This application pertains to *Staphylococcus pseudocarpa*. Staphylococcus pseudoxylosus AT8 can achieve a degradation rate of over 90% for trimethoprim and tilmicosin, and over 78% for enrofloxacin. Furthermore, this application enriches the antibiotic-degrading bacterial strain library, promotes the control of new antibiotic pollutants in livestock and poultry manure and farmland soil, and facilitates the resource-based treatment and safe utilization of solid waste, demonstrating broad application prospects.

The present invention provides the use of 5-fluoro-2'-deoxycytidine (CDP-3) in the preparation of antibacterial drugs. The present invention discovers for the first time that CDP-3 can inhibit the synthesis of bacterial phosphatidylglycerol by targeting bacterial phosphatidylglycerol phosphate synthase, and has antibacterial and broad-spectrum synergistic activities. Its MICs against Staphylococcus aureus and Streptococcus are 4 μg / mL and 8 μg / mL respectively, and the MIC 90 against methicillin-resistant Staphylococcus aureus is 8 μg / mL, and the MIC against hypervirulent Klebsiella pneumoniae is 16 μg / mL. In addition, CDP-3 can significantly enhance the antibacterial efficacy of antibiotics such as clindamycin, tilmicosin, tetracycline, florfenicol, gentamicin, chloramphenicol, rifampicin and ofloxacin against multi-drug resistant Gram-negative bacteria, and has broad-spectrum synergistic activity. Sub-inhibitory concentrations of CDP-3 can not only inhibit bacterial growth, but also reduce bacterial biofilm formation, and inhibit virulence and pathogenicity. CDP-3 not only increases the survival rate of Galleria mellonella infected with MRSA, but also shows excellent therapeutic efficacy against animal models infected with multi-drug resistant Gram-negative bacteria.

The invention provides a water-soluble tilmicosin premix for treating pleuropneumonia, mycoplasma infection and ileitis and a preparation method of the water-soluble tilmicosin premix, and belongs to the field of medicaments for livestock and poultry. The water-soluble tilmicosin premixing agent is prepared from tilmicosin base, modified chitosan, citric acid, polyvinylpyrrolidone, polyethylene glycol 6000, poloxamer 188, lactose, silicon dioxide and hydroxypropyl-beta-cyclodextrin. The modified chitosan is prepared by grafting vitamin E succinate and quaternary ammonium salt chitosan, has amphipathy, can effectively embed tilmicosin, and improves the water solubility and stability of tilmicosin. The water-soluble tilmicosin premix disclosed by the invention is good in water solubility, good in palatability and high in bioavailability, can be administrated in a water drinking or material stirring manner, and can be used for treating pleuropneumonia, mycoplasma infection and ileitis of livestock and poultry such as pigs and chickens.

Automatic atomization drug delivery treatment system after cough early warning of farm pig group. The present application relates to the field of automatic breeding technology of animal husbandry. The system comprises a cough monitoring unit, a central control unit, an atomization execution unit and an environmental control linkage unit. The cough monitoring unit carries a data interface to collect and identify pig cough sounds in real time. When the cough frequency reaches the preset threshold, the atomization execution unit is automatically triggered. The atomization execution unit uses high-pressure cutting atomization technology to produce 3-7 μm atomized particles, and carries a cold power system that does not heat throughout the process. The atomization duration is set to 60-120 min. The environmental control linkage unit automatically reduces the ventilation volume to 30%-50% of the normal level during atomization to prolong the retention time of the liquid medicine. The special treatment agent is a water-soluble atomized preparation of tilmicosin. The present application realizes the unmanned automatic linkage of cough monitoring and atomization drug delivery, solves the three defects of large atomized particles, heating damage to drug efficacy during atomization process and the inability of cough monitoring and atomization control to be linked, and significantly improves the drug deposition efficiency through environmental control cooperation.

The invention relates to the technical field of antibiotic detection, and particularly discloses a sample treatment method for detecting macrolide antibiotics in a plant source matrix sample. The sample treatment method provided by the invention comprises the following steps: uniformly mixing a plant source matrix sample with a buffer solution, adding acidified acetonitrile, salting out and absorbing water by using a mixed inorganic salt, and purifying by using an adsorbent comprising anhydrous magnesium sulfate, an aliphatic diamine derivative and O-terminated octadecyl bonded silica gel to obtain the treated plant source matrix sample. Through the systematic design of extraction-separation-purification, the chemical properties of the target object and the characteristics of the plant matrix are combined, and the synergistic effect of the buffer solution, acidified acetonitrile, salting-out and multiple adsorbents is utilized, so that matrix interference can be effectively removed, the target object is stable, and efficient extraction of macrolide antibiotics is realized. The method is suitable for detecting the macrolide antibiotics such as lincomycin, tilmicosin and erythromycin in the plant source matrix.

The tilmicosin production reaction device comprises a machine body, supporting legs are arranged on the outer walls of the two sides of the lower end of the machine body respectively, connecting rods are arranged on the opposite faces of the supporting legs respectively, a material conveying pipe is arranged between the connecting rods, a material distributing pipe is arranged on the upper portion of the machine body, and a material outlet pipe is arranged on the lower portion of the machine body. And the lower end of the material distributing pipe is inserted between the limiting plates. According to the tilmicosin production reaction device for veterinary drugs, the material distributing pipe and the limiting plate form a spiral locking structure through the multiple sets of first bolts, so that tilmicosin powder raw materials can fall onto the screen in a dispersed mode through the material distributing pipe, and then the follow-up dissolution rate of the raw materials is increased through dispersed feeding; and the stirring rods form a rotating structure in the machine body through the rotating shaft under the action of the motor, so that the tilmicosin powder raw material can be further ensured to be fed dispersedly through the screen with an inclined structure, and meanwhile, the dissolution effect of the tilmicosin powder raw material is prevented from being influenced by impurities or cakes through the screen.

The invention relates to the technical field of pharmaceutical preparations, and particularly discloses a microencapsulated tilmicosin premix and a preparation method thereof, and the microencapsulated tilmicosin premix comprises the following raw materials: tilmicosin, a composite capsule material, an emulsifier, a stabilizer, a suspending aid and livestock and poultry feed acceptable auxiliary materials. The preparation method of the microencapsulated tilmicosin premix comprises the following steps: step 1, adding a composite capsule material into deionized water, and stirring and dispersing to obtain a capsule material solution; step 2, adding tilmicosin, an emulsifier and a stabilizer into the capsule material solution, and homogenizing to obtain a medicine dispersion liquid; step 3, performing spray drying on the medicine dispersion liquid, and sieving after drying to obtain microcapsule particles; and step 4, adding the microcapsule particles, a suspending aid and livestock and poultry feed acceptable auxiliary materials into a mixing machine, and mixing to obtain the microencapsulated tilmicosin premix. The prepared microencapsulated tilmicosin premix is high in encapsulation efficiency, excellent in targeting performance and high in stability, and adapts to livestock and poultry feed application scenes.

The application relates to the technical field of veterinary medicine preparation, and discloses a veterinary medicine for treating poultry respiratory diseases, which is prepared from timicosin, ambroxol, propylene glycol, glycyrrhizic acid, L-arginine, anhydrous betaine and purified water; the preparation process is as follows: the timicosin and the ambroxol are dissolved in the propylene glycol to obtain a hydrophobic phase; the glycyrrhizic acid, the L-arginine and the anhydrous betaine are dissolved in the purified water to obtain a hydrophilic phase; the hydrophobic phase is pumped into the hydrophilic phase under the conditions of heating and high shearing, and then, heat preservation is carried out, and then, the temperature is lowered to room temperature, purified water is added, and filtration is carried out, so that the finished product is obtained. The veterinary medicine uses glycyrrhizic acid and L-arginine to establish a supramolecular network, the hydrophobic drug is wrapped in the inner core to mask the bitter taste, and the anhydrous betaine is promoted to form an electrostatic hydration shell in the periphery through quenching, so that the problems of insufficient water intake and drug intake of poultry are solved, drug skeleton degradation is prevented, the intestinal mucosa osmotic pressure under the heat stress state is adjusted, and the transmembrane absorption rate is improved.