51 results about "Tylosin" patented technology

The invention discloses a method for preparing a liquid fertilizer and improving crop yield by using tylosin waste liquid, and belongs to the technical field of agricultural waste resource utilization, and the method comprises the following steps: S1, preparing a novel liquid fertilizer: mixing the tylosin waste liquid with an antibiotic degradation agent for reaction, centrifuging, and collecting supernatant; adding nutrient substances and a mixed bacterial agent into the centrifugate for fermentation treatment, and filtering to obtain filtrate, namely the novel liquid fertilizer; s2, taking crop seeds, and soaking the crop seeds in a soaking solution before planting; s3, in the seed bud stage and the seedling stage, leaf surface spraying is conducted; wherein the antibiotic degradation agent is prepared by reacting modified silicon dioxide with sulfhydrylated sodium carboxymethyl cellulose. According to the method, resource utilization of the tylosin waste liquid is achieved, environmental pollution and use of chemical fertilizers are reduced, the agricultural production cost is reduced, and an effective way is provided for agricultural sustainable development.

The invention relates to the field of fertilizers, in particular to a method for preparing an organic fertilizer by anaerobic fermentation of tylosin dregs, which comprises the following steps: (1) electron beam irradiation treatment; (2) adding the mushroom dreg powder into an ethanol solution, and treating with a coupling agent KH-550 to obtain aminated mushroom dregs; (3) preparing to obtain phosphorylated hydroxypropyl methyl cellulose; (4) mixing the aminated mushroom dregs and the phosphorylated hydroxypropyl methyl cellulose in an organic solvent for reaction to obtain phosphorylated hydroxypropyl methyl cellulose composite active mushroom dregs; and (5) composting to finally obtain the organic fertilizer. According to the organic fertilizer and the preparation method thereof, the tylosin mushroom dregs are subjected to modification treatment, anaerobic fermentation treatment and aerobic fermentation treatment, so that the content of antibiotic tylosin in the finally prepared organic fertilizer is greatly reduced, the industrial requirements can be met, the content of organic matters in soil can be increased, the soil structure can be improved, and the soil fertility can be improved; the plant growth is facilitated.

The application discloses a method for producing yellow fermentation acid slow-release organic fertilizer by using tylosin bacterial residue, and the method comprises the following steps: crushing the tylosin bacterial residue into powder by using a cell wall breaking machine; adding gamma-manganese dioxide and lignite in sequence, stirring and mixing uniformly, and fully contacting and reacting; irradiating and treating the obtained mixed system by using a high-energy electron beam to obtain yellow fermentation acid organic fertilizer; and compounding the obtained product with diatomite, and granulating after cooling to obtain the yellow fermentation acid slow-release organic fertilizer. The harmless treatment and resource utilization of the tylosin bacterial residue are realized, the product meets the actual production and application requirements, the product has good fertilizer efficiency, the residual antibiotics are removed synchronously, and the product has the dual functions of fertilizer efficiency and antibiotic removal.

The invention discloses a nitrogen source feeding control method in a tylosin fermentation process, and belongs to the technical field of tylosin fermentation. A nitrogen source material supplementing control method in the tylosin fermentation process comprises two stages of dynamic material supplementing and comprises the following specific steps that in the early stage of fermentation, after a fermentation tank runs for 20 hours, continuous culture is conducted, the titer of tylosin in fermentation liquor is detected regularly, an ammonium nitrate solution starts to be supplemented, and the pH value of the fermentation liquor is stabilized between 6.55 and 6.80 through material supplementing. According to the nitrogen source feeding control method in the tylosin fermentation process, key parameters such as pH, dissolved oxygen, biomass and ammonia nitrogen concentration are collected in real time through cooperative work of the PLC system and the online sensor, the ammonium nitrate feeding rate is dynamically adjusted, nitrogen source supply is highly matched with thallus metabolism requirements, nitrogen source waste or insufficiency is avoided, and the production efficiency is improved. The nitrogen source utilization efficiency is improved; and a carbon source synchronous supplementing strategy is combined to stabilize a fermentation liquid environment, promote rapid growth of thalli and prolong a stable growth period.

The invention discloses a compound liquid fertilizer containing tylosin finished product liquid, and belongs to the technical field of fertilizer preparation. Comprising a tylosin metabolite complex TDMC, humic acid, a seaweed extract, urea, monopotassium phosphate, potassium sulfate, an organic acid compound, polyaspartic acid, a polyol compound, a trace element compound, a stabilizer, an antifreeze agent, an antioxidant, a preservative and deionized water. The tylosin metabolite complex TDMC is a metabolite which is obtained by converting tylosin through a biological conversion method and has no antibacterial activity but retains a plant immunity induction function. According to the compound liquid fertilizer containing tylosin metabolite derivatives, a TDMC compound is obtained through a biotransformation technology, stabilization and slow release of active ingredients are achieved through a multifunctional chelating carrier system, and the compound liquid fertilizer has remarkable advantages in the aspects of product stability, plant immune induction, disease resistance, growth promoting effect and the like; and compared with the traditional method, the performance is improved by about 40-50%.

The invention belongs to the technical field of medical chemistry, and discloses a 5-O-carbon mould amine tylosin lactone derivative with a double-target combined antibacterial effect as well as a preparation method and application of the 5-O-carbon mould amine tylosin lactone derivative. The derivative is a compound as shown in a formula I, and pharmaceutically acceptable salt, eutectic, tautomer, polymorphic substance, solvate, prodrug or isotope labeled compound thereof. The compound can act on a bacterial ribosome 50S subunit and DNA topoisomerase IV at the same time, and shows an excellent antibacterial effect in an antibacterial activity test, and the minimum inhibitory concentration (MIC) reaches 0.0625 mu g / mL. The compound has the advantages of being high in antibacterial activity, wide in antibacterial spectrum and capable of achieving rapid sterilization, and a new choice is provided for developing drugs with antibacterial activity.

The application discloses a fermentation treatment equipment and method for tylosin waste liquid, relates to the technical field of tylosin waste liquid treatment, and aims to solve the technical problem of low dewatering efficiency caused by the 'water film wrapping' of high-viscosity sludge. The application realizes efficient and deep dewatering of sludge through four-stage progressive dewatering modes and collaborative design. The equipment is based on the cooperation of centrifugation and extrusion, and can quickly separate free water and shallow bound water. Then, the 'corner shearing' is used to fill the dewatering blind area and ensure the uniformity of sludge dewatering in the whole area. Subsequently, the'multi-point concave-convex extrusion' is used to tear the deep water film and improve the dewatering depth. Finally, the 'needle column puncture' is used to remove the residual water film and completely reduce the water content of the sludge. The four-stage dewatering modes are seamlessly linked, and the self-adaptive sealing structure and energy collaborative design are used, so that the sludge leakage and secondary pollution are avoided, the unit treatment energy consumption is reduced, and the pain points of the traditional equipment, such as incomplete, uneven and low-efficiency dewatering, are solved.

Disclosed in the present invention are a Tylosin derivative, a preparation method therefor, and a use thereof. The tylosin derivative provided by the present invention has the structure shown in formula 1. Also provided in the present invention are a veterinary drug composition and a pharmaceutical preparation containing the tylosin derivative, and a use of the veterinary drug composition and the pharmaceutical preparation in the treatment or prevention of bacterial infection diseases in animals. Also provided in the present invention is a feed additive comprising the tylosin derivative.

The invention discloses a tylosin sensing biosensor, a construction method thereof and MphR mutant protein based on the tylosin sensing biosensor, and belongs to the technical field of gene engineering. The technical problem to be solved by the invention is how to detect tylosin. In order to solve the technical problem, the invention provides the biosensor for sensing tylosin, the biosensor can express an MphR31D1 protein and / or a coding gene containing the MphR31D1 protein, and the MphR31D1 protein can be a protein with an amino acid sequence of SEQ ID NO: 1. The invention also provides a preparation method of the biosensor, and application of the MphR31D1 protein and related biological materials thereof in detection of tylosin. According to the invention, escherichia coli Top10 is taken as a chassis bacterium, a macrolide compound transcriptional regulation factor MphR is taken as a starting template, and the tylosin whole-cell biosensor is successfully constructed through protein directed evolution.

The application discloses application of tegaserod maleate in preparation of an antibacterial drug synergist, and proves that the tegaserod maleate can produce a synergistic antibacterial effect with a plurality of commonly used antibacterial drugs (including tylosin, tetracycline, enrofloxacin, ampicillin and the like), can significantly enhance the antibacterial activity of the antibacterial drugs on gram-negative bacteria, and reduce the drug resistance of bacteria to the antibacterial drugs; the antibacterial composition composed of the tegaserod maleate and the antibacterial drugs can be used for preparing a drug for treating escherichia coli infection, and the synergistic antibacterial mechanism is mainly to destroy the inner and outer membrane permeability of bacteria, regulate the proton motive force of bacteria, inhibit the activity of bacterial efflux pumps, interfere with the energy metabolism homeostasis of bacteria and induce the bacteria to produce oxidative stress. The application provides a new technical strategy for the treatment of clinical escherichia coli infection, and has important clinical application value and industrialization potential.

The invention provides bacillus subtilis, a microbial agent of the bacillus subtilis and application of the bacillus subtilis to prevention and treatment of cucumber bacterial angular leaf spot. The bacillus subtilis is a bacillus subtilis strain A45, and the preservation number is CGMCC No.33289. The invention further provides a preparation method of the bacillus subtilis. The microbial agent of the bacterial strain can be used for efficiently inhibiting the growth of the Pseudomonas amygdali pv.lachrymans (Pseudomonas amygdali pv.lachrymans) of the cucumber bacterial angular leaf spot pathogen, and can be used for efficiently inhibiting the growth of the Pseudomonas amygdali pv.lachrymans. The strain code not only can be used for synthesizing known gene clusters of antibacterial substances such as abeleventin, bacitene, tripeptide tripeptide, catechol siderophore, pexamic acid, subtilosin A, lysobacin and the like, but also can be used for synthesizing a new gene cluster of surfactin, 1-carbapaen-2-em-3-carboxylic acid and tylosin A as well as a gene cluster synthesized by two brand-new terpenoids. The microbial agent disclosed by the invention not only can prevent cucumber angular leaf spot, but also has a remarkable promoting effect on cucumber growth.

The application discloses a tylosin polyketide synthase acyltransferase mutant and application thereof, relates to the field of bioengineering, and comprises a first mutant, a second mutant or a third mutant; the amino acid sequences are shown in SEQ ID NO. 2, SEQ ID NO. 3 or SEQ ID NO. 4 respectively. The application discloses a coding gene of the mutant, a recombination carrier and a recombination bacteria and application thereof in catalyzing malonyl-CoA hydrolysis. The application discloses a soluble expression method of tylosin polyketide synthase acyltransferase domain TylAT5 protein, which comprises culturing bacterial bodies, eluting target proteins and further purifying the target proteins by using fast protein liquid chromatography. The application provides a recombination escherichia coli which can efficiently express acyltransferase mutants, and the efficiency of catalyzing malonyl-CoA of the recombination escherichia coli is 2.6 times higher than that of a wild type.

The invention relates to the technical field of a process for producing tylosin by fermentation, and discloses a fermentation process for producing tylosin by utilizing streptomyces fradiae, aiming at the problems that a carbon source is insufficient due to the fact that lipase is not massively synthesized at the early stage of fermentation and a manner of supplementing vegetable oil at the middle and later stages of fermentation is not timely and inaccurate. The culture medium for fermentation is prepared from the following raw materials: vegetable oil, lipase, a carbon source and the like; the preparation method of the culture medium comprises the following steps: taking vegetable oil, a carbon source, a nitrogen source and inorganic salt, adding water for dissolving, putting into a fermentation tank, adjusting the pH value to 7.0-8.0, heating while stirring, adding lipase, carrying out enzymolysis, supplementing water after enzymolysis, and sterilizing to obtain the culture medium. Vegetable oil and lipase are added when the culture medium is prepared, so that the process of decomposing the vegetable oil to generate diglyceride and the like is completed in advance, seamless switching of a carbon source from starch to the vegetable oil is immediately realized when the starch is exhausted, and the overall growth speed of the strain and the product synthesis efficiency are integrally improved.

The invention discloses a method for rapidly detecting various veterinary drug residues in a dairy product by adopting a biochip. The method comprises the following steps: (a) recovering a dairy product sample to be detected, a biochip detection card and micropores to room temperature; (b) selecting a biochip detection card and a micropore, marking the biochip detection card and the micropore, and preheating the micropore on a dry heater; (c) adding a to-be-detected sample into the micropores, repeatedly sucking, beating and uniformly mixing until a micropore solid is completely dissolved, and incubating; (d) transferring all the liquid in the micropores into a sample adding hole of the biochip detection card, uniformly mixing and reacting; and (e) interpreting a result. The method for detecting the chloramphenicol, the lincomycin, the tylosin and the tilmicosin in the dairy product is low in detection limit and can meet the dairy product limit requirement formulated in the national standard.

The invention discloses a composition for treating porcine reproductive and respiratory syndrome and a feeding method thereof, and belongs to the technical field of veterinary medicines. The composition is composed of veterinary acetylisovaleryltylosin tartrate, isatis root, medium chain triglyceride and glycerol monolaurate according to a specific mass ratio, the veterinary acetylisovaleryltylosin tartrate can specifically inhibit replication of porcine reproductive and respiratory syndrome viruses, the isatis root has the effects of resisting viruses and enhancing the immunity of the organism, and the medium chain triglyceride has the effect of inhibiting replication of the porcine reproductive and respiratory syndrome viruses. The mixture of medium-chain triglyceride and glycerol monolaurate not only can improve the solubility and bioavailability of effective components, but also can synergistically enhance the antiviral effect. Through scientific compounding, the composition disclosed by the invention can effectively prevent and treat the porcine reproductive and respiratory syndrome, and is high in safety and free from drug residue risk; the composition is suitable for popularization and application in large-scale pig farms and free-range households, and effectively solves the problems of single effect, low bioavailability, easy generation of drug resistance and the like of existing drugs for preventing and treating porcine reproductive and respiratory syndrome.

The invention discloses a novel method for extracting tylosin fermentation liquor. The method comprises the following steps: step 1, pH regulation pretreatment of the tylosin fermentation liquor; step 2, performing tubular ultrafiltration membrane filtration on the pretreated fermentation liquor; and step 3, introducing the filtrate obtained in the step 2 into an extraction separator for extraction. The tubular ultrafiltration membrane with the molecular weight cut-off of 10000 is used for replacing traditional plate frame filtration, impurities such as macromolecular protein and pigment are cut off by means of the selective separation characteristic of the membrane, and the quality of filtrate is greatly improved. High-purity filtrate is combined with butyl acetate counter-current extraction, efficient separation is achieved by means of solubility difference, the emulsification phenomenon is remarkably reduced, and the extraction yield stably exceeds 93%. The phase inversion liquid is concentrated by a nanofiltration membrane, the titer is accurately controlled to be more than 200,000 U / mL, compared with the traditional evaporation concentration, the high-temperature degradation of the tylosin is reduced, the energy consumption is reduced, and the concentration yield is more than 99%.

The invention discloses a fermentation culture process for improving the titer of tylosin, which comprises the following steps: S1, fermentation preparation: adding a proper amount of drinking water into a barrel filled with materials, uniformly stirring and mixing, pouring the material liquid into a fermentation tank, and then adding a proper amount of drinking water; heating the tank to 60.0-80.0 DEG C, gelatinizing for 20 minutes, unscrewing an inoculation cap, adding water through an inoculation opening to fix the volume, tightening the inoculation cap, sampling, measuring the pH value, adjusting the pH value, then adding a small amount of calcium carbonate dissolved by water and soybean oil, and starting self-control sterilization; after sterilization is completed, the temperature of the tank is reduced to 30.0 + / -2.0 DEG C before inoculation is prepared, inoculation is carried out according to a plan, and a seed solution is transferred into a fermentation tank. According to the method, the gelatinization temperature, the sterilization parameters and the inoculation conditions are accurately regulated and controlled, so that a proper initial environment is provided for thallus growth; the tank temperature, the air flow and the stirring rotating speed are regulated and controlled in sections, and the metabolic requirements of thalli in different growth stages are met.

The invention provides a preparation method of diethylaminoethyl isothiourea salt and a preparation method of tiamulin, and belongs to the technical field of organic synthesis. The invention provides a preparation method of diethylaminoethyl isothiourea salt, which comprises the following steps: mixing diethylaminoethanol and thiourea, and carrying out alkylation reaction to obtain the diethylaminoethyl isothiourea salt. The diethylaminoethyl alcohol and the thiourea are mixed for alkylation reaction to obtain the diethylaminoethyl isothiourea salt, the diethylaminoethyl isothiourea salt is prepared through a one-pot method, the preparation efficiency is high, the steps are simple, the raw materials are easy to obtain, and the cost is low. The invention further provides a preparation method of the tiamulin, the steps are simple, the method is more suitable for industrial production, and the purity of the tiamulin is improved. Data of the embodiment shows that the purity of the tiamulin prepared by the method reaches 97% or above.

The application discloses a phosphorus-sulfur synergistic antibiotic bacterial residue biochar, a preparation method and application thereof, and belongs to the technical field of biochar. The application takes penicillin bacterial residue and high-sulfur wet-process phosphoric acid as raw materials, and prepares biochar through phosphorus-sulfur synergistic modification and applies the biochar to adsorption of antibiotic wastewater, so that efficient purification of the antibiotic wastewater and full-resource utilization of the bacterial residue are realized. The method innovatively uses cheap high-sulfur wet-process phosphoric acid to replace traditional strong acid (hydrochloric acid, sulfuric acid, etc.) for pretreatment, and the obtained acid hydrolysis solution can be directly used for preparing fertilizer, thereby simultaneously reducing pyrolysis cost and improving nitrogen utilization rate. The phosphorus-sulfur in-situ modification process makes the carbon yield of the biochar increase by 10%, the specific surface area increase by 45%, and the resistant genes in the bacterial residue are completely eliminated. The prepared biochar has an antibiotic removal rate of more than 99% for antibiotic wastewater such as enrofloxacin and tylosin, and the wastewater COD and ammonia nitrogen removal rates reach 96% and 71% respectively, so that the high-value utilization of antibiotic bacterial residue is realized while the environmental safety is ensured.

The invention discloses a preparation method and application of an artificially synthesized charcoal immobilized degrading enzyme material based on tylosin degrading bacteria, and relates to the technical field of material synthesis and modification. The method comprises the following steps: adding the biochar into a tylosin degradation intracellular enzyme solution, fully mixing, putting into a constant-temperature oscillation shaking table, and adsorbing at the temperature of 29-31 DEG C; after the adsorption is finished, centrifuging and collecting the precipitate, washing with a phosphate buffer solution, centrifuging again, and washing and centrifuging twice again to obtain the artificially synthesized charcoal immobilized degrading enzyme material based on the tylosin degrading bacteria. The artificially synthesized charcoal immobilized degrading enzyme material based on the tylosin degrading bacteria is applied to synchronous removal of antibiotics and resistance genes in soil. The invention can obtain the preparation method and the application of the artificially synthesized charcoal immobilized degrading enzyme material based on the tylosin degrading bacteria.

The invention discloses an application of a tylosin derivative in preparation of a medicine for resisting poxviridae viruses. It is found that the tylosin derivative T3 can reduce the replication level of LSDV, and the derivative is low in toxicity, has no toxic or side effect on MDBK cells, can serve as an inhibitor for efficiently resisting bovine nodular skin disease viruses, provides a powerful theoretical basis and a practical basis for further research and development of antiviral drugs, and has good application prospects. Wide application prospects are realized in the fields of breeding industry and biological medicines.

The invention discloses an MphR protein mutant combined with tylosin and a tylosin whole-cell biosensor constructed on the basis of the MphR protein mutant. The invention provides an MphR24G2 protein, and the MphR24G2 protein is shown as SEQ ID NO: 1. According to the invention, a macrolide compound transcriptional regulation factor MphR is taken as a starting template, mutant protein capable of being combined with tylosin is successfully found through protein directed evolution, and a tylosin whole-cell biosensor is further constructed. The tylosin whole-cell biosensor constructed by the invention can be used as a high-throughput screening tool to be applied to breeding and improvement of tylosin production strains, so that the screening flux and breeding speed of the tylosin production strains are greatly improved, and the tylosin whole-cell biosensor has a good application prospect.

A method for increasing the fermentation titer of Acetyl-isovaleryl tylosin. An oxygen carrier and a surfactant are added in the fermentation process of Acetyl-isovaleryl tylosin, and Streptomyces thermotolerans is used for fermentation in a fermentation culture medium to produce the Acetyl-isovaleryl tylosin. The level of dissolved oxygen of a fermentation liquid in different fermentation periods is effectively regulated and controlled, so that the oxygen dissolving capacity of the Streptomyces thermotolerans is improved, and synthesis of the Acetyl-isovaleryl tylosin is promoted, thereby remarkably increasing the fermentation titer of the Acetyl-isovaleryl tylosin.

This invention relates to the field of immunology, and more particularly to a hybridoma cell line and its secreted monoclonal antibody against the metamizole residue marker 4-methylaminoantipyrine (MAA), and its applications. This invention uses 4-aminoantipyrine to react with methyl 4-bromomethylbenzoate, followed by alkaline hydrolysis to obtain a hapten (Formula I). ​​An immunogen is then synthesized and used to immunize mice. After cell fusion and screening, a hybridoma cell line capable of secreting a monoclonal antibody recognizing MAA is prepared, named Metamizole-6A4. This hybridoma cell line has a high efficiency in secreting monoclonal antibodies. The secreted monoclonal antibody can accurately detect MAA, IC50, and other markers. 50 The concentration is 4.06 ng / mL, with high specificity. Cross-reactivity with aminopyrine and its metabolites 4-aminoantipyrine, 4-formamidoantipyrine, 4-acetamidoantipyrine, sulfadiazine, norfloxacin, lincomycin, tylosin, penicillin G, streptomycin, and gentamicin is less than 5%. It can accurately detect MAA residues in food. Formula I.

The application discloses application of tylosin derivatives in preparation of anti-poxviridae virus drugs. It is found by the application that tylosin derivative T3 can reduce the replication level of LSDV, the derivative has low toxicity, has no toxic side effect on MDBK cells, can be used as an inhibitor for efficiently resisting bovine tuberculous dermatosis virus, and provides a strong theoretical basis and practical basis for further anti-virus drug research and development, and has wide application prospects in the breeding industry and the biological medicine field.

The invention relates to the technical field of protein engineering, in particular to a method for improving the yield and purity of tylosin A by constructing a mutant of tylosin reductase tylAKR and reducing the affinity of the mutant to a substrate. The Y53F mutant is obtained by carrying out directional mutation on amino acid at a key active site of the tylosin reductase, so that the activity of the tylosin reductase is inhibited, the reduction activity of the tylosin reductase to a substrate tylosin (component A) is reduced, the yield and purity of the tylosin (component A) are effectively improved, the dosage of veterinary antibiotics is reduced, and the production cost is reduced. The safety of animal husbandry and public health is guaranteed, and remarkable economic benefits and social benefits are achieved.

The invention relates to the technical field of protein engineering, in particular to a method for improving the yield and purity of tylosin A by constructing a mutant of tylosin reductase tylAKR and reducing the affinity of the mutant to a substrate. The W55A mutant is obtained by carrying out directional mutation on amino acid at a key active site of the tylosin reductase, so that the activity of the tylosin reductase is inhibited, the reduction activity of the tylosin reductase to a substrate tylosin (component A) is reduced, the yield and purity of the tylosin (component A) are effectively improved, the dosage of veterinary antibiotics is reduced, and the cost is reduced. The safety of animal husbandry and public health is guaranteed, and remarkable economic benefits and social benefits are achieved.

The invention relates to the technical field of protein engineering, in particular to a method for improving the yield and purity of tylosin A by constructing a mutant of tylosin reductase tylAKR and reducing the affinity of the mutant to a substrate. The amino acid of the key active site of the tylosin reductase is subjected to directional mutation to obtain the M21A mutant, so that the activity of the tylosin reductase is inhibited, the reduction activity of the tylosin reductase to a substrate tylosin (component A) is reduced, the yield and purity of the tylosin (component A) are effectively improved, the dosage of veterinary antibiotics is reduced, and the cost is reduced. The safety of animal husbandry and public health is guaranteed, and remarkable economic benefits and social benefits are achieved.

The invention relates to the technical field of organic fertilizers, and discloses a method for preparing an organic fertilizer from tylosin mushroom dregs and lincomycin mushroom dregs, and the method comprises the following steps: after the tylosin mushroom dregs are crushed, the tylosin mushroom dregs are degraded to obtain tylosin mushroom dreg powder; the method comprises the following steps: crushing lincomycin fungus residues, and degrading the lincomycin fungus residues to obtain lincomycin fungus residue powder; mixing the tylosin mushroom dreg powder, the lincomycin mushroom dreg powder, the corn straw, the waste vegetable leaves and the livestock and poultry manure, and performing fermentation treatment; the tylosin mushroom dregs and the lincomycin mushroom dregs are used as raw materials of the organic fertilizer, and the quality and the effect of the final fertilizer are remarkably improved through a reasonable degradation treatment and fermentation process. Through fermentation treatment and addition of the functional bacterial liquid, the number of effective flora in a fermentation product is increased, richer beneficial microorganisms can be provided for soil, and improvement of the soil structure and improvement of the soil fertility are facilitated.

The invention discloses a production process of tilmicosin phosphate, which comprises the following steps: adding tartaric acid into a tylosin extracting solution to control the tylosin extracting solution, filtering to obtain a tylosin intermediate, and controlling the extracting solution to leave a butyl acetate phase; the preparation method comprises the following steps: adding 3.5-dimethyl piperidine and formic acid, controlling an acid extract to leave a water phase, adding activated carbon, stirring, filtering mother liquor by using a centrifugal machine to obtain a wet crude product, adding the wet crude product and phosphoric acid into purified water, and drying to obtain a tilmicosin phosphate finished product. The production process is simplified, the production time is shortened, the procedures of decoloration, desolvation, concentration and spray drying of the tylosin intermediate are not needed any more, the time is shortened by about 24 hours, meanwhile, after the steps are reduced, the cost of personnel, materials, energy and the like can be saved, the tylosin intermediate is directly extracted, and subsequent tilmicosin phosphate production is performed after the extraction reaction, so that the production efficiency is improved, and the production cost is reduced. And the total yield can be improved by 3-4%.