33 results about "Tylosin" patented technology

The application discloses a method for producing yellow fermentation acid slow-release organic fertilizer by using tylosin bacterial residue, and the method comprises the following steps: crushing the tylosin bacterial residue into powder by using a cell wall breaking machine; adding gamma-manganese dioxide and lignite in sequence, stirring and mixing uniformly, and fully contacting and reacting; irradiating and treating the obtained mixed system by using a high-energy electron beam to obtain yellow fermentation acid organic fertilizer; and compounding the obtained product with diatomite, and granulating after cooling to obtain the yellow fermentation acid slow-release organic fertilizer. The harmless treatment and resource utilization of the tylosin bacterial residue are realized, the product meets the actual production and application requirements, the product has good fertilizer efficiency, the residual antibiotics are removed synchronously, and the product has the dual functions of fertilizer efficiency and antibiotic removal.

The invention discloses a nitrogen source feeding control method in a tylosin fermentation process, and belongs to the technical field of tylosin fermentation. A nitrogen source material supplementing control method in the tylosin fermentation process comprises two stages of dynamic material supplementing and comprises the following specific steps that in the early stage of fermentation, after a fermentation tank runs for 20 hours, continuous culture is conducted, the titer of tylosin in fermentation liquor is detected regularly, an ammonium nitrate solution starts to be supplemented, and the pH value of the fermentation liquor is stabilized between 6.55 and 6.80 through material supplementing. According to the nitrogen source feeding control method in the tylosin fermentation process, key parameters such as pH, dissolved oxygen, biomass and ammonia nitrogen concentration are collected in real time through cooperative work of the PLC system and the online sensor, the ammonium nitrate feeding rate is dynamically adjusted, nitrogen source supply is highly matched with thallus metabolism requirements, nitrogen source waste or insufficiency is avoided, and the production efficiency is improved. The nitrogen source utilization efficiency is improved; and a carbon source synchronous supplementing strategy is combined to stabilize a fermentation liquid environment, promote rapid growth of thalli and prolong a stable growth period.

The invention belongs to the technical field of medical chemistry, and discloses a 5-O-carbon mould amine tylosin lactone derivative with a double-target combined antibacterial effect as well as a preparation method and application of the 5-O-carbon mould amine tylosin lactone derivative. The derivative is a compound as shown in a formula I, and pharmaceutically acceptable salt, eutectic, tautomer, polymorphic substance, solvate, prodrug or isotope labeled compound thereof. The compound can act on a bacterial ribosome 50S subunit and DNA topoisomerase IV at the same time, and shows an excellent antibacterial effect in an antibacterial activity test, and the minimum inhibitory concentration (MIC) reaches 0.0625 mu g / mL. The compound has the advantages of being high in antibacterial activity, wide in antibacterial spectrum and capable of achieving rapid sterilization, and a new choice is provided for developing drugs with antibacterial activity.

The application discloses a fermentation treatment equipment and method for tylosin waste liquid, relates to the technical field of tylosin waste liquid treatment, and aims to solve the technical problem of low dewatering efficiency caused by the 'water film wrapping' of high-viscosity sludge. The application realizes efficient and deep dewatering of sludge through four-stage progressive dewatering modes and collaborative design. The equipment is based on the cooperation of centrifugation and extrusion, and can quickly separate free water and shallow bound water. Then, the 'corner shearing' is used to fill the dewatering blind area and ensure the uniformity of sludge dewatering in the whole area. Subsequently, the'multi-point concave-convex extrusion' is used to tear the deep water film and improve the dewatering depth. Finally, the 'needle column puncture' is used to remove the residual water film and completely reduce the water content of the sludge. The four-stage dewatering modes are seamlessly linked, and the self-adaptive sealing structure and energy collaborative design are used, so that the sludge leakage and secondary pollution are avoided, the unit treatment energy consumption is reduced, and the pain points of the traditional equipment, such as incomplete, uneven and low-efficiency dewatering, are solved.

The invention discloses a tylosin sensing biosensor, a construction method thereof and MphR mutant protein based on the tylosin sensing biosensor, and belongs to the technical field of gene engineering. The technical problem to be solved by the invention is how to detect tylosin. In order to solve the technical problem, the invention provides the biosensor for sensing tylosin, the biosensor can express an MphR31D1 protein and / or a coding gene containing the MphR31D1 protein, and the MphR31D1 protein can be a protein with an amino acid sequence of SEQ ID NO: 1. The invention also provides a preparation method of the biosensor, and application of the MphR31D1 protein and related biological materials thereof in detection of tylosin. According to the invention, escherichia coli Top10 is taken as a chassis bacterium, a macrolide compound transcriptional regulation factor MphR is taken as a starting template, and the tylosin whole-cell biosensor is successfully constructed through protein directed evolution.

The application discloses application of tegaserod maleate in preparation of an antibacterial drug synergist, and proves that the tegaserod maleate can produce a synergistic antibacterial effect with a plurality of commonly used antibacterial drugs (including tylosin, tetracycline, enrofloxacin, ampicillin and the like), can significantly enhance the antibacterial activity of the antibacterial drugs on gram-negative bacteria, and reduce the drug resistance of bacteria to the antibacterial drugs; the antibacterial composition composed of the tegaserod maleate and the antibacterial drugs can be used for preparing a drug for treating escherichia coli infection, and the synergistic antibacterial mechanism is mainly to destroy the inner and outer membrane permeability of bacteria, regulate the proton motive force of bacteria, inhibit the activity of bacterial efflux pumps, interfere with the energy metabolism homeostasis of bacteria and induce the bacteria to produce oxidative stress. The application provides a new technical strategy for the treatment of clinical escherichia coli infection, and has important clinical application value and industrialization potential.

The application discloses a tylosin polyketide synthase acyltransferase mutant and application thereof, relates to the field of bioengineering, and comprises a first mutant, a second mutant or a third mutant; the amino acid sequences are shown in SEQ ID NO. 2, SEQ ID NO. 3 or SEQ ID NO. 4 respectively. The application discloses a coding gene of the mutant, a recombination carrier and a recombination bacteria and application thereof in catalyzing malonyl-CoA hydrolysis. The application discloses a soluble expression method of tylosin polyketide synthase acyltransferase domain TylAT5 protein, which comprises culturing bacterial bodies, eluting target proteins and further purifying the target proteins by using fast protein liquid chromatography. The application provides a recombination escherichia coli which can efficiently express acyltransferase mutants, and the efficiency of catalyzing malonyl-CoA of the recombination escherichia coli is 2.6 times higher than that of a wild type.

The invention discloses a method for rapidly detecting various veterinary drug residues in a dairy product by adopting a biochip. The method comprises the following steps: (a) recovering a dairy product sample to be detected, a biochip detection card and micropores to room temperature; (b) selecting a biochip detection card and a micropore, marking the biochip detection card and the micropore, and preheating the micropore on a dry heater; (c) adding a to-be-detected sample into the micropores, repeatedly sucking, beating and uniformly mixing until a micropore solid is completely dissolved, and incubating; (d) transferring all the liquid in the micropores into a sample adding hole of the biochip detection card, uniformly mixing and reacting; and (e) interpreting a result. The method for detecting the chloramphenicol, the lincomycin, the tylosin and the tilmicosin in the dairy product is low in detection limit and can meet the dairy product limit requirement formulated in the national standard.

The invention discloses a composition for treating porcine reproductive and respiratory syndrome and a feeding method thereof, and belongs to the technical field of veterinary medicines. The composition is composed of veterinary acetylisovaleryltylosin tartrate, isatis root, medium chain triglyceride and glycerol monolaurate according to a specific mass ratio, the veterinary acetylisovaleryltylosin tartrate can specifically inhibit replication of porcine reproductive and respiratory syndrome viruses, the isatis root has the effects of resisting viruses and enhancing the immunity of the organism, and the medium chain triglyceride has the effect of inhibiting replication of the porcine reproductive and respiratory syndrome viruses. The mixture of medium-chain triglyceride and glycerol monolaurate not only can improve the solubility and bioavailability of effective components, but also can synergistically enhance the antiviral effect. Through scientific compounding, the composition disclosed by the invention can effectively prevent and treat the porcine reproductive and respiratory syndrome, and is high in safety and free from drug residue risk; the composition is suitable for popularization and application in large-scale pig farms and free-range households, and effectively solves the problems of single effect, low bioavailability, easy generation of drug resistance and the like of existing drugs for preventing and treating porcine reproductive and respiratory syndrome.

The invention discloses a novel method for extracting tylosin fermentation liquor. The method comprises the following steps: step 1, pH regulation pretreatment of the tylosin fermentation liquor; step 2, performing tubular ultrafiltration membrane filtration on the pretreated fermentation liquor; and step 3, introducing the filtrate obtained in the step 2 into an extraction separator for extraction. The tubular ultrafiltration membrane with the molecular weight cut-off of 10000 is used for replacing traditional plate frame filtration, impurities such as macromolecular protein and pigment are cut off by means of the selective separation characteristic of the membrane, and the quality of filtrate is greatly improved. High-purity filtrate is combined with butyl acetate counter-current extraction, efficient separation is achieved by means of solubility difference, the emulsification phenomenon is remarkably reduced, and the extraction yield stably exceeds 93%. The phase inversion liquid is concentrated by a nanofiltration membrane, the titer is accurately controlled to be more than 200,000 U / mL, compared with the traditional evaporation concentration, the high-temperature degradation of the tylosin is reduced, the energy consumption is reduced, and the concentration yield is more than 99%.

The invention discloses a fermentation culture process for improving the titer of tylosin, which comprises the following steps: S1, fermentation preparation: adding a proper amount of drinking water into a barrel filled with materials, uniformly stirring and mixing, pouring the material liquid into a fermentation tank, and then adding a proper amount of drinking water; heating the tank to 60.0-80.0 DEG C, gelatinizing for 20 minutes, unscrewing an inoculation cap, adding water through an inoculation opening to fix the volume, tightening the inoculation cap, sampling, measuring the pH value, adjusting the pH value, then adding a small amount of calcium carbonate dissolved by water and soybean oil, and starting self-control sterilization; after sterilization is completed, the temperature of the tank is reduced to 30.0 + / -2.0 DEG C before inoculation is prepared, inoculation is carried out according to a plan, and a seed solution is transferred into a fermentation tank. According to the method, the gelatinization temperature, the sterilization parameters and the inoculation conditions are accurately regulated and controlled, so that a proper initial environment is provided for thallus growth; the tank temperature, the air flow and the stirring rotating speed are regulated and controlled in sections, and the metabolic requirements of thalli in different growth stages are met.

The application discloses a phosphorus-sulfur synergistic antibiotic bacterial residue biochar, a preparation method and application thereof, and belongs to the technical field of biochar. The application takes penicillin bacterial residue and high-sulfur wet-process phosphoric acid as raw materials, and prepares biochar through phosphorus-sulfur synergistic modification and applies the biochar to adsorption of antibiotic wastewater, so that efficient purification of the antibiotic wastewater and full-resource utilization of the bacterial residue are realized. The method innovatively uses cheap high-sulfur wet-process phosphoric acid to replace traditional strong acid (hydrochloric acid, sulfuric acid, etc.) for pretreatment, and the obtained acid hydrolysis solution can be directly used for preparing fertilizer, thereby simultaneously reducing pyrolysis cost and improving nitrogen utilization rate. The phosphorus-sulfur in-situ modification process makes the carbon yield of the biochar increase by 10%, the specific surface area increase by 45%, and the resistant genes in the bacterial residue are completely eliminated. The prepared biochar has an antibiotic removal rate of more than 99% for antibiotic wastewater such as enrofloxacin and tylosin, and the wastewater COD and ammonia nitrogen removal rates reach 96% and 71% respectively, so that the high-value utilization of antibiotic bacterial residue is realized while the environmental safety is ensured.

The invention discloses an application of a tylosin derivative in preparation of a medicine for resisting poxviridae viruses. It is found that the tylosin derivative T3 can reduce the replication level of LSDV, and the derivative is low in toxicity, has no toxic or side effect on MDBK cells, can serve as an inhibitor for efficiently resisting bovine nodular skin disease viruses, provides a powerful theoretical basis and a practical basis for further research and development of antiviral drugs, and has good application prospects. Wide application prospects are realized in the fields of breeding industry and biological medicines.

The invention discloses an MphR protein mutant combined with tylosin and a tylosin whole-cell biosensor constructed on the basis of the MphR protein mutant. The invention provides an MphR24G2 protein, and the MphR24G2 protein is shown as SEQ ID NO: 1. According to the invention, a macrolide compound transcriptional regulation factor MphR is taken as a starting template, mutant protein capable of being combined with tylosin is successfully found through protein directed evolution, and a tylosin whole-cell biosensor is further constructed. The tylosin whole-cell biosensor constructed by the invention can be used as a high-throughput screening tool to be applied to breeding and improvement of tylosin production strains, so that the screening flux and breeding speed of the tylosin production strains are greatly improved, and the tylosin whole-cell biosensor has a good application prospect.

This invention relates to the field of immunology, and more particularly to a hybridoma cell line and its secreted monoclonal antibody against the metamizole residue marker 4-methylaminoantipyrine (MAA), and its applications. This invention uses 4-aminoantipyrine to react with methyl 4-bromomethylbenzoate, followed by alkaline hydrolysis to obtain a hapten (Formula I). ​​An immunogen is then synthesized and used to immunize mice. After cell fusion and screening, a hybridoma cell line capable of secreting a monoclonal antibody recognizing MAA is prepared, named Metamizole-6A4. This hybridoma cell line has a high efficiency in secreting monoclonal antibodies. The secreted monoclonal antibody can accurately detect MAA, IC50, and other markers. 50 The concentration is 4.06 ng / mL, with high specificity. Cross-reactivity with aminopyrine and its metabolites 4-aminoantipyrine, 4-formamidoantipyrine, 4-acetamidoantipyrine, sulfadiazine, norfloxacin, lincomycin, tylosin, penicillin G, streptomycin, and gentamicin is less than 5%. It can accurately detect MAA residues in food. Formula I.

The application discloses application of tylosin derivatives in preparation of anti-poxviridae virus drugs. It is found by the application that tylosin derivative T3 can reduce the replication level of LSDV, the derivative has low toxicity, has no toxic side effect on MDBK cells, can be used as an inhibitor for efficiently resisting bovine tuberculous dermatosis virus, and provides a strong theoretical basis and practical basis for further anti-virus drug research and development, and has wide application prospects in the breeding industry and the biological medicine field.

The invention relates to the technical field of protein engineering, in particular to a method for improving the yield and purity of tylosin A by constructing a mutant of tylosin reductase tylAKR and reducing the affinity of the mutant to a substrate. The Y53F mutant is obtained by carrying out directional mutation on amino acid at a key active site of the tylosin reductase, so that the activity of the tylosin reductase is inhibited, the reduction activity of the tylosin reductase to a substrate tylosin (component A) is reduced, the yield and purity of the tylosin (component A) are effectively improved, the dosage of veterinary antibiotics is reduced, and the production cost is reduced. The safety of animal husbandry and public health is guaranteed, and remarkable economic benefits and social benefits are achieved.

The invention relates to the technical field of protein engineering, in particular to a method for improving the yield and purity of tylosin A by constructing a mutant of tylosin reductase tylAKR and reducing the affinity of the mutant to a substrate. The W55A mutant is obtained by carrying out directional mutation on amino acid at a key active site of the tylosin reductase, so that the activity of the tylosin reductase is inhibited, the reduction activity of the tylosin reductase to a substrate tylosin (component A) is reduced, the yield and purity of the tylosin (component A) are effectively improved, the dosage of veterinary antibiotics is reduced, and the cost is reduced. The safety of animal husbandry and public health is guaranteed, and remarkable economic benefits and social benefits are achieved.

The invention relates to the technical field of protein engineering, in particular to a method for improving the yield and purity of tylosin A by constructing a mutant of tylosin reductase tylAKR and reducing the affinity of the mutant to a substrate. The amino acid of the key active site of the tylosin reductase is subjected to directional mutation to obtain the M21A mutant, so that the activity of the tylosin reductase is inhibited, the reduction activity of the tylosin reductase to a substrate tylosin (component A) is reduced, the yield and purity of the tylosin (component A) are effectively improved, the dosage of veterinary antibiotics is reduced, and the cost is reduced. The safety of animal husbandry and public health is guaranteed, and remarkable economic benefits and social benefits are achieved.

The invention discloses a production process of tilmicosin phosphate, which comprises the following steps: adding tartaric acid into a tylosin extracting solution to control the tylosin extracting solution, filtering to obtain a tylosin intermediate, and controlling the extracting solution to leave a butyl acetate phase; the preparation method comprises the following steps: adding 3.5-dimethyl piperidine and formic acid, controlling an acid extract to leave a water phase, adding activated carbon, stirring, filtering mother liquor by using a centrifugal machine to obtain a wet crude product, adding the wet crude product and phosphoric acid into purified water, and drying to obtain a tilmicosin phosphate finished product. The production process is simplified, the production time is shortened, the procedures of decoloration, desolvation, concentration and spray drying of the tylosin intermediate are not needed any more, the time is shortened by about 24 hours, meanwhile, after the steps are reduced, the cost of personnel, materials, energy and the like can be saved, the tylosin intermediate is directly extracted, and subsequent tilmicosin phosphate production is performed after the extraction reaction, so that the production efficiency is improved, and the production cost is reduced. And the total yield can be improved by 3-4%.

This invention belongs to the field of bioengineering technology, specifically relating to a recombinant plasmid for tylosin synthesis, an engineered bacterial strain, and its preparation method. The genetically engineered bacterial strain (Streptomyces freundii G3-F2) was deposited at the China Center for Type Culture Collection (CCTCC) on February 24, 2025, with accession number CCTCC NO: M 2025278. This invention achieves site-specific recombination, utilizes an optimized ZouA gene DNA amplification system to construct the recombinant plasmid, and realizes… tulle Multiple copies of the F gene were increased, resulting in the preparation of *Streptomyces freundii* G3-F2. This significantly improved the rate of methyltransferase enzymatic reaction and achieved efficient conversion of component C. Combined with low-temperature fermentation, the rate and efficiency of tylosin synthesis were greatly enhanced. The strain achieved a titer of 18500 U / mL in a 50L tank under 28℃ low-temperature culture conditions, which can significantly reduce production costs.

This invention belongs to the field of photocatalytic material preparation and antibiotic degradation technology, specifically relating to a ZIF-67(C) / g-C3N4 heterojunction photocatalyst, its preparation method, and its application in antibiotic degradation. This invention involves pre-calcining ZIF-67 to generate CN-framework-encapsulated zero-valent cobalt, which is then mixed with melamine and calcined to form the ZIF-67(C) / g-C3N4 heterojunction photocatalyst. This catalyst not only contains CN-framework-encapsulated zero-valent cobalt, Co3O4, and g-C3N4, but also forms a Co3O4 / g-C3N4 p-n heterojunction, exhibiting a synergistic activation effect on PMS, thus enhancing the catalyst's catalytic degradation effect on antibiotics. At a low dosage of 0.2 g / L, this catalyst can essentially completely degrade tylosin in water within 10 minutes.

The invention discloses tiamulin fumarate tablets capable of improving bioavailability and a preparation method of the tiamulin fumarate tablets, and belongs to the technical field of veterinary drug preparations. Every 100 kg of the tiamulin fumarate tablet is mainly prepared from the following raw materials in parts by weight: 10 to 30 kg of tiamulin fumarate, 28 to 35 kg of Soluplus, 1.2 to 2.0 kg of silicon dioxide, 0.5 to 1.1 kg of N-trimethyl chitosan (TMC), 16 to 20 kg of HPMC-AS, 3.2 to 4.0 kg of triethyl citrate, 16 to 25 kg of microcrystalline cellulose (PH102) and 3.1 to 4.3 kg of croscarmellose sodium. According to the invention, a composite enteric-solubilization-infiltration promotion system is adopted, and the innovative process of a solubilization inner core adopts co-grinding of a main drug and Soluplus, so that the water solubility of the drug is fully improved; the enteric-coated layer adopts HPMC-AS (hydroxypropyl methylcellulose acetate succinate) to solve the problem of gastric acid degradation and reduce the gastric degradation rate, so that drugs are accurately released in intestinal tracts, and the bioavailability is improved; the penetration enhancer chitosan derivative N-trimethyl chitosan (TMC) is added, so that the intestinal mucosa permeability is enhanced, and the intestinal absorption rate is increased.

This invention belongs to the fields of biomedicine and bioengineering technology, specifically relating to mutants of *Streptomyces freundii* TylF methyltransferase and their application in tylosin production. The amino acid sequences of mutants and combined mutants of the TylF methyltransferase amino acid sites, and the corresponding nucleotide sequences of the encoding genes are provided. These mutants significantly improve the enzyme activity characteristics of TylF: (1) the mutants have higher TylF methyltransferase activity, which can significantly increase tylosin yield; (2) the mutants have a lower optimal catalytic temperature (30℃), which allows for efficient conversion of tylosin C to tylosin A (tylosin C / A conversion) without raising the temperature to 38℃ in the later stages of fermentation, reducing production energy consumption. This invention has application value in the genetic engineering modification of macrolide antibiotic biosynthetic strains such as tylosin and in the industrial production of macrolide antibiotics such as tylosin.

This invention discloses a method for detecting the bioavailability of antibiotics in soil using continuous solvent extraction-solid phase extraction-liquid chromatography-tandem mass spectrometry. The method first uses continuous solvent extraction to extract the bioactive and extractable antibiotic components from the soil. Then, a solid phase extraction column is used for sample purification. Finally, multiple reaction monitoring (MRM) mode of liquid chromatography-tandem mass spectrometry is used to determine the concentrations of various antibiotics, including chlortetracycline, doxycycline, oxytetracycline, tetracycline, sulfadiazine, sulfadiazine, sulfamethoxazole, ciprofloxacin, enrofloxacin, norfloxacin, ofloxacin, azithromycin, erythromycin, roxithromycin, tylosin, trimethoprim, and lincomycin. This invention combines continuous solvent extraction with solid phase extraction, coupled with liquid chromatography-tandem mass spectrometry detection, to achieve accurate evaluation of trace antibiotic bioavailability under complex matrix conditions.

This invention relates to a method for the extraction and purification of tylosin. The method employs a polyamide-macroporous resin combination for adsorption and decolorization, combined with a gradient cooling secondary crystallization method. This establishes a novel process for separating and purifying tylosin from fermentation broth, optimizing the tylosin extraction and purification process and making it more suitable for industrial production. The extraction and purification method of this invention achieves a product yield of over 97.5% and a purity of over 95%.

A tylosin derivative and a preparation method and application thereof are provided. The tylosin derivative has a structure represented by in Formula 1. A veterinary pharmaceutical composition and a pharmaceutical preparation containing the tylosin derivative, and use of them in treating or preventing bacterial infection diseases in animals are also provided. Moreover, a feed additive including the tylosin derivative is provided.

The invention belongs to the technical field of tylosin degradation, and particularly discloses an application of a Kurthia gibsonii TYL-A1 degrading enzyme in degradation of tylosin, for the first time, a target gene GM002829 is screened from Kurthia gibsonii TYL-A1, and alpha / beta hydrolase Rcmd-2829 is successfully obtained by constructing a recombinant bacterium containing the gene for expression and purification. The result shows that the optimal reaction conditions of Rcmd-2829 are pH 9.0 and temperature of 30 DEG C; in combination with molecular docking, molecular dynamics simulation and site-directed mutagenesis experiments, the key effect of Ser-102 residues in the process of degrading TYL by Rcmd-2829 is proved; in addition, the Rcmd-2829 also shows a good capability of removing TYL in actual sewage; according to the invention, the molecular mechanism (dependent on Ser-102) of the alpha / beta hydrolase Rcmd-2829 for degrading the TYL is clarified for the first time, and a foundation is laid for developing a high-efficiency enzymatic repair technology.

This invention discloses a process for preparing organic fertilizer from tylosin bacterial residue, relating to the field of organic fertilizer technology. In this invention, the tylosin bacterial residue is first pretreated with modified carboxymethyl cellulose and hydrogen peroxide. Then, the pretreated residue is mixed with auxiliary materials, aerobic composted, dried, pulverized, and granulated to obtain organic fertilizer. The modified carboxymethyl cellulose is obtained by co-precipitating sodium carboxymethyl cellulose and iron ion solution, followed by reaction with 3-chloro-2-hydroxypropyltrimethylammonium chloride. This invention's process for preparing organic fertilizer from tylosin bacterial residue achieves high tylosin removal efficiency and short processing time, realizing the reduction, harmlessness, and resource utilization of tylosin bacterial residue.

The invention discloses a method for producing tylosin through high-level fermentation. The method sequentially comprises slant culture, shake flask seed culture, first-stage seed tank culture, second-stage seed tank culture and fermentation tank culture, in the fermentation tank culture process, polypeptide powder is used as a nitrogen source for tylosin fermentation, the viscosity change of fermentation liquor in the fermentation process is controlled, operation is easy, control is easy, the fermentation unit of tylosin can be greatly increased, and the fermentation cost of tylosin can be greatly reduced.