17 results about "Tylosin" patented technology

The application discloses a method for producing yellow fermentation acid slow-release organic fertilizer by using tylosin bacterial residue, and the method comprises the following steps: crushing the tylosin bacterial residue into powder by using a cell wall breaking machine; adding gamma-manganese dioxide and lignite in sequence, stirring and mixing uniformly, and fully contacting and reacting; irradiating and treating the obtained mixed system by using a high-energy electron beam to obtain yellow fermentation acid organic fertilizer; and compounding the obtained product with diatomite, and granulating after cooling to obtain the yellow fermentation acid slow-release organic fertilizer. The harmless treatment and resource utilization of the tylosin bacterial residue are realized, the product meets the actual production and application requirements, the product has good fertilizer efficiency, the residual antibiotics are removed synchronously, and the product has the dual functions of fertilizer efficiency and antibiotic removal.

The invention belongs to the technical field of medical chemistry, and discloses a 5-O-carbon mould amine tylosin lactone derivative with a double-target combined antibacterial effect as well as a preparation method and application of the 5-O-carbon mould amine tylosin lactone derivative. The derivative is a compound as shown in a formula I, and pharmaceutically acceptable salt, eutectic, tautomer, polymorphic substance, solvate, prodrug or isotope labeled compound thereof. The compound can act on a bacterial ribosome 50S subunit and DNA topoisomerase IV at the same time, and shows an excellent antibacterial effect in an antibacterial activity test, and the minimum inhibitory concentration (MIC) reaches 0.0625 mu g / mL. The compound has the advantages of being high in antibacterial activity, wide in antibacterial spectrum and capable of achieving rapid sterilization, and a new choice is provided for developing drugs with antibacterial activity.

The application discloses a fermentation treatment equipment and method for tylosin waste liquid, relates to the technical field of tylosin waste liquid treatment, and aims to solve the technical problem of low dewatering efficiency caused by the 'water film wrapping' of high-viscosity sludge. The application realizes efficient and deep dewatering of sludge through four-stage progressive dewatering modes and collaborative design. The equipment is based on the cooperation of centrifugation and extrusion, and can quickly separate free water and shallow bound water. Then, the 'corner shearing' is used to fill the dewatering blind area and ensure the uniformity of sludge dewatering in the whole area. Subsequently, the'multi-point concave-convex extrusion' is used to tear the deep water film and improve the dewatering depth. Finally, the 'needle column puncture' is used to remove the residual water film and completely reduce the water content of the sludge. The four-stage dewatering modes are seamlessly linked, and the self-adaptive sealing structure and energy collaborative design are used, so that the sludge leakage and secondary pollution are avoided, the unit treatment energy consumption is reduced, and the pain points of the traditional equipment, such as incomplete, uneven and low-efficiency dewatering, are solved.

The application discloses application of tegaserod maleate in preparation of an antibacterial drug synergist, and proves that the tegaserod maleate can produce a synergistic antibacterial effect with a plurality of commonly used antibacterial drugs (including tylosin, tetracycline, enrofloxacin, ampicillin and the like), can significantly enhance the antibacterial activity of the antibacterial drugs on gram-negative bacteria, and reduce the drug resistance of bacteria to the antibacterial drugs; the antibacterial composition composed of the tegaserod maleate and the antibacterial drugs can be used for preparing a drug for treating escherichia coli infection, and the synergistic antibacterial mechanism is mainly to destroy the inner and outer membrane permeability of bacteria, regulate the proton motive force of bacteria, inhibit the activity of bacterial efflux pumps, interfere with the energy metabolism homeostasis of bacteria and induce the bacteria to produce oxidative stress. The application provides a new technical strategy for the treatment of clinical escherichia coli infection, and has important clinical application value and industrialization potential.

The application discloses a tylosin polyketide synthase acyltransferase mutant and application thereof, relates to the field of bioengineering, and comprises a first mutant, a second mutant or a third mutant; the amino acid sequences are shown in SEQ ID NO. 2, SEQ ID NO. 3 or SEQ ID NO. 4 respectively. The application discloses a coding gene of the mutant, a recombination carrier and a recombination bacteria and application thereof in catalyzing malonyl-CoA hydrolysis. The application discloses a soluble expression method of tylosin polyketide synthase acyltransferase domain TylAT5 protein, which comprises culturing bacterial bodies, eluting target proteins and further purifying the target proteins by using fast protein liquid chromatography. The application provides a recombination escherichia coli which can efficiently express acyltransferase mutants, and the efficiency of catalyzing malonyl-CoA of the recombination escherichia coli is 2.6 times higher than that of a wild type.

The invention discloses a method for rapidly detecting various veterinary drug residues in a dairy product by adopting a biochip. The method comprises the following steps: (a) recovering a dairy product sample to be detected, a biochip detection card and micropores to room temperature; (b) selecting a biochip detection card and a micropore, marking the biochip detection card and the micropore, and preheating the micropore on a dry heater; (c) adding a to-be-detected sample into the micropores, repeatedly sucking, beating and uniformly mixing until a micropore solid is completely dissolved, and incubating; (d) transferring all the liquid in the micropores into a sample adding hole of the biochip detection card, uniformly mixing and reacting; and (e) interpreting a result. The method for detecting the chloramphenicol, the lincomycin, the tylosin and the tilmicosin in the dairy product is low in detection limit and can meet the dairy product limit requirement formulated in the national standard.

The invention discloses a novel method for extracting tylosin fermentation liquor. The method comprises the following steps: step 1, pH regulation pretreatment of the tylosin fermentation liquor; step 2, performing tubular ultrafiltration membrane filtration on the pretreated fermentation liquor; and step 3, introducing the filtrate obtained in the step 2 into an extraction separator for extraction. The tubular ultrafiltration membrane with the molecular weight cut-off of 10000 is used for replacing traditional plate frame filtration, impurities such as macromolecular protein and pigment are cut off by means of the selective separation characteristic of the membrane, and the quality of filtrate is greatly improved. High-purity filtrate is combined with butyl acetate counter-current extraction, efficient separation is achieved by means of solubility difference, the emulsification phenomenon is remarkably reduced, and the extraction yield stably exceeds 93%. The phase inversion liquid is concentrated by a nanofiltration membrane, the titer is accurately controlled to be more than 200,000 U / mL, compared with the traditional evaporation concentration, the high-temperature degradation of the tylosin is reduced, the energy consumption is reduced, and the concentration yield is more than 99%.

The invention discloses a fermentation culture process for improving the titer of tylosin, which comprises the following steps: S1, fermentation preparation: adding a proper amount of drinking water into a barrel filled with materials, uniformly stirring and mixing, pouring the material liquid into a fermentation tank, and then adding a proper amount of drinking water; heating the tank to 60.0-80.0 DEG C, gelatinizing for 20 minutes, unscrewing an inoculation cap, adding water through an inoculation opening to fix the volume, tightening the inoculation cap, sampling, measuring the pH value, adjusting the pH value, then adding a small amount of calcium carbonate dissolved by water and soybean oil, and starting self-control sterilization; after sterilization is completed, the temperature of the tank is reduced to 30.0 + / -2.0 DEG C before inoculation is prepared, inoculation is carried out according to a plan, and a seed solution is transferred into a fermentation tank. According to the method, the gelatinization temperature, the sterilization parameters and the inoculation conditions are accurately regulated and controlled, so that a proper initial environment is provided for thallus growth; the tank temperature, the air flow and the stirring rotating speed are regulated and controlled in sections, and the metabolic requirements of thalli in different growth stages are met.

This invention relates to the field of immunology, and more particularly to a hybridoma cell line and its secreted monoclonal antibody against the metamizole residue marker 4-methylaminoantipyrine (MAA), and its applications. This invention uses 4-aminoantipyrine to react with methyl 4-bromomethylbenzoate, followed by alkaline hydrolysis to obtain a hapten (Formula I). ​​An immunogen is then synthesized and used to immunize mice. After cell fusion and screening, a hybridoma cell line capable of secreting a monoclonal antibody recognizing MAA is prepared, named Metamizole-6A4. This hybridoma cell line has a high efficiency in secreting monoclonal antibodies. The secreted monoclonal antibody can accurately detect MAA, IC50, and other markers. 50 The concentration is 4.06 ng / mL, with high specificity. Cross-reactivity with aminopyrine and its metabolites 4-aminoantipyrine, 4-formamidoantipyrine, 4-acetamidoantipyrine, sulfadiazine, norfloxacin, lincomycin, tylosin, penicillin G, streptomycin, and gentamicin is less than 5%. It can accurately detect MAA residues in food. Formula I.

The application discloses application of tylosin derivatives in preparation of anti-poxviridae virus drugs. It is found by the application that tylosin derivative T3 can reduce the replication level of LSDV, the derivative has low toxicity, has no toxic side effect on MDBK cells, can be used as an inhibitor for efficiently resisting bovine tuberculous dermatosis virus, and provides a strong theoretical basis and practical basis for further anti-virus drug research and development, and has wide application prospects in the breeding industry and the biological medicine field.

This invention belongs to the field of bioengineering technology, specifically relating to a recombinant plasmid for tylosin synthesis, an engineered bacterial strain, and its preparation method. The genetically engineered bacterial strain (Streptomyces freundii G3-F2) was deposited at the China Center for Type Culture Collection (CCTCC) on February 24, 2025, with accession number CCTCC NO: M 2025278. This invention achieves site-specific recombination, utilizes an optimized ZouA gene DNA amplification system to construct the recombinant plasmid, and realizes… tulle Multiple copies of the F gene were increased, resulting in the preparation of *Streptomyces freundii* G3-F2. This significantly improved the rate of methyltransferase enzymatic reaction and achieved efficient conversion of component C. Combined with low-temperature fermentation, the rate and efficiency of tylosin synthesis were greatly enhanced. The strain achieved a titer of 18500 U / mL in a 50L tank under 28℃ low-temperature culture conditions, which can significantly reduce production costs.

This invention belongs to the field of photocatalytic material preparation and antibiotic degradation technology, specifically relating to a ZIF-67(C) / g-C3N4 heterojunction photocatalyst, its preparation method, and its application in antibiotic degradation. This invention involves pre-calcining ZIF-67 to generate CN-framework-encapsulated zero-valent cobalt, which is then mixed with melamine and calcined to form the ZIF-67(C) / g-C3N4 heterojunction photocatalyst. This catalyst not only contains CN-framework-encapsulated zero-valent cobalt, Co3O4, and g-C3N4, but also forms a Co3O4 / g-C3N4 p-n heterojunction, exhibiting a synergistic activation effect on PMS, thus enhancing the catalyst's catalytic degradation effect on antibiotics. At a low dosage of 0.2 g / L, this catalyst can essentially completely degrade tylosin in water within 10 minutes.

The invention discloses tiamulin fumarate tablets capable of improving bioavailability and a preparation method of the tiamulin fumarate tablets, and belongs to the technical field of veterinary drug preparations. Every 100 kg of the tiamulin fumarate tablet is mainly prepared from the following raw materials in parts by weight: 10 to 30 kg of tiamulin fumarate, 28 to 35 kg of Soluplus, 1.2 to 2.0 kg of silicon dioxide, 0.5 to 1.1 kg of N-trimethyl chitosan (TMC), 16 to 20 kg of HPMC-AS, 3.2 to 4.0 kg of triethyl citrate, 16 to 25 kg of microcrystalline cellulose (PH102) and 3.1 to 4.3 kg of croscarmellose sodium. According to the invention, a composite enteric-solubilization-infiltration promotion system is adopted, and the innovative process of a solubilization inner core adopts co-grinding of a main drug and Soluplus, so that the water solubility of the drug is fully improved; the enteric-coated layer adopts HPMC-AS (hydroxypropyl methylcellulose acetate succinate) to solve the problem of gastric acid degradation and reduce the gastric degradation rate, so that drugs are accurately released in intestinal tracts, and the bioavailability is improved; the penetration enhancer chitosan derivative N-trimethyl chitosan (TMC) is added, so that the intestinal mucosa permeability is enhanced, and the intestinal absorption rate is increased.

A tylosin derivative and a preparation method and application thereof are provided. The tylosin derivative has a structure represented by in Formula 1. A veterinary pharmaceutical composition and a pharmaceutical preparation containing the tylosin derivative, and use of them in treating or preventing bacterial infection diseases in animals are also provided. Moreover, a feed additive including the tylosin derivative is provided.

The invention belongs to the technical field of tylosin degradation, and particularly discloses an application of a Kurthia gibsonii TYL-A1 degrading enzyme in degradation of tylosin, for the first time, a target gene GM002829 is screened from Kurthia gibsonii TYL-A1, and alpha / beta hydrolase Rcmd-2829 is successfully obtained by constructing a recombinant bacterium containing the gene for expression and purification. The result shows that the optimal reaction conditions of Rcmd-2829 are pH 9.0 and temperature of 30 DEG C; in combination with molecular docking, molecular dynamics simulation and site-directed mutagenesis experiments, the key effect of Ser-102 residues in the process of degrading TYL by Rcmd-2829 is proved; in addition, the Rcmd-2829 also shows a good capability of removing TYL in actual sewage; according to the invention, the molecular mechanism (dependent on Ser-102) of the alpha / beta hydrolase Rcmd-2829 for degrading the TYL is clarified for the first time, and a foundation is laid for developing a high-efficiency enzymatic repair technology.

The invention discloses a tylosin degrading strain and application thereof in compost, and belongs to the technical field of microbial degradation of antibiotics. According to the experiment, two strains of tylosin degrading bacteria are separated from pig manure, and the two strains of tylosin degrading bacteria are identified as Sphingobacterium kyonggiense TYL-1 and Empedobacter falsenii TYL2. The obtained tylosin degrading bacterium TYL2 is separated from pig manure of a pig farm, and the obtained strain can be directly used for degrading tylosin in wastewater and compost of the pig farm.

The invention discloses a method for producing fulvic acid slow-release organic fertilizer by utilizing tylosin mushroom dregs. The method comprises the following steps: crushing the tylosin mushroom dregs into powder by using a wall breaking machine; sequentially adding gamma-manganese dioxide and lignite, uniformly stirring and mixing, and fully contacting and reacting; carrying out irradiation treatment on the obtained mixed system by utilizing a high-energy electron beam to obtain the fulvic acid type organic fertilizer. And compounding the obtained product with diatomite, cooling, and granulating to obtain the fulvic acid slow-release organic fertilizer. According to the method, innocent treatment and resource utilization of the tylosin fungi residues are achieved, the product meets the actual production and application requirements, the good fertilizer efficiency is achieved, the residual antibiotics are synchronously removed, and the double functions of achieving the fertilizer efficiency and removing the antibiotics are achieved.