170 results about "Tylosin" patented technology

The present invention relates to the technical fields of organic chemistry and pharmaceutical chemistry, concretely discloses a synthetic method for a known compound tylosin. The synthetic method comprises the following steps: taking Azithromycin A as a raw material, the protected Azithromycin A can be obtained by Cbz-Cl and then oxidized through a modified Pfitznor-Moffat method, the protected ketone by Cbz can be obtained, ketone is reacted through Wittig-Horner, keto is conversed into alkenyl to obtain the protected alkene, the protected alkene is oxidized to obtain an epoxy compound protected by Cbz, the epoxy compound protected by Cbz is deprotected to obtain a deprotected epoxy compound under the catalysis of Pd / C, the deprotected epoxy compound is reacted with n-propylamine and is performed a ring-opening to obtain a tylosin crude product, the tylosin crude product and acid are carried out a salt forming and purifying, then tylosin can be obtained by resolving. The synthetic route is changed by the method of the invention, the super low-temperature is not employed in the route, and the tylosin enables the effective industrialization production.

The present invention relates to a high-throughput detection chip for drug resistance gene of bacteria, and an application thereof. The detection chip comprises 117 gene probes, the drug resistance gene probes are selected from 17 categories of drug resistance genes, which comprise extended spectrum beta-lactamase, cephalosporinase, carbapenemase, integrase gene, tetracycline resistance gene, aminoglycoside resistance gene, disinfectant resistance gene, erythromycin resistance gene, macrolide efflux gene, vancomycin resistance gene, multidrug resistance efflux pump gene, mupirocin resistance gene, sulfanilamide resistance gene, tylosin resistance gene, fluoroquinolone resistance gene, chloramphenicol acetyltransferase and commonly-used genetic engineering vector resistance gene. The chip is adopted for detecting the resistance gene of the pathogenic bacteria. The chip is characterized in that: the chip comprises (1) 117-oligonucleotide probe composition and quality control probes of 17 categories of the drug resistance genes; (2) probe arrays, wherein the oligonucleotide probes are solidified on the vector material through arm molecules to form the probe arrays.

A test paper used for quickly detecting residue of tylosin consists of support layer, absorption layer and protection membrane. It is featured as forming said absorption layer by setting fiber layer, absorption gold mark antibody fiber layer, cellulose membrane layer and water absorption layer in sequence from test end to handle end; setting linear detection signet and linear comparison signet on said cellulose membrane layer and arranging said detection signet and said comparison signet to be in parallel mode.

The invention discloses application of papermaking sludge based biological carbon in removal of antibiotics or heavy metals and the antibiotics from a water body and belongs to the technical field ofbiomass treatment and water treatment. The papermaking sludge based biological carbon has larger specific area and a larger pore size and can efficiently remove combined pollution of copper, zinc, arsenic, sulfadimidine, tylosin and roxithromycin. The biological carbon is simple in preparation technology, significant and stable in effect, environment-friendly and free from secondary pollution, andhas a wide application value in a heavy metal / antibiotic pollution repair aspect in the water body. Paper mill sludge difficult to treat is recycled; the environmental pollution problems of wastewater containing the heavy metals and the antibiotics are solved; and therefore, the application has important environmental and economic benefits. The biological carbon has a huge market and an application potential in waste recycling and sewage treatment.

The present invention provides a tildipirosin preparation method, tylosin as a starting reactant is used for preparation of 23-hydroxyl-5-O-mycaminose-tylosin lactone, 14-aldehyde-5-O-mycaminose-tylosin lactone is further prepared, and finally tildipirosin(IV) is prepared; by determining of reasonable oxidizing conditions, the use of expensive iodine can be avoided, the reaction conditions can be reduced; by controlling of the reaction conditions, two times of hydrolysis reaction can be performed in one pot, the operation steps can be reduced, the technical purpose of overall cost reduction can be achieved, and the method is less in step, simple in process, low in cost, and suitable for large-scale industrial production need.

The invention belongs to the veterinary medicine residual analysis and immunity analysis technique field and specifically relates to an enzyme-linked immunity method and the reagent kit thereof which can discern specificity monoclonal antibodies of tylosin and tilmicosin and detect the residuals of tylosin and tilmicosin at the same time; the monoclonal antibodies in the invention is secreted from hybridoma cell strain P3C4 established by the applicant; the hybridoma cell strain is preserved in China Center of Type Culture Collection; the number of preservation of the hybridoma cell strain is CCTCC No: C200719; the invention discloses the preparation method and enzyme-linked immunity detection method of the monoclonal antibody, coating antigen and immunogen. Compared with the prior art, the monoclonal antibody prepared in the invention can discern tylosin and tilmicosin at the same time and add the detecting object in the prior art. The reagent kit and the method in the invention has the advantages of simple, convenience, swiftness, sensitivity and accuracy; furthermore, the reagent kit and method in the invention can detect the residuals of tylosin and tilmicosin in an animal edibility tissue at the same time.

A method for synthesizing tilmicosin and tilmicosin phosphate through using a tylosin broth is disclosed. The method is characterized in that the method comprises the following process steps: preprocessing the tylosin broth to obtain a tylosin filtrate; allowing the tylosin filtrate to undergo extraction, ammonification, reextraction, hydrolysis, acidification liquid extraction and vacuum drying to obtain tilmicosin; and allowing the obtained tilmicosin to undergo phosphorylation and spray drying to obtain tilmicosin phosphate. The method, which directly extracts the tylosin broth which is the initial raw material to synthesize the tilmicosin and the tilmicosin phosphate, allows the production procedure to be simplified, the product production time to be shortened, reaction conditions of the whole technology to be mild, the operation to be simple, the production process to be safe and reliable, and the organic solvent loss to be less, and has the advantages of energy saving and consumption reduction, high finished product yield, low production cost, high working efficiency and the like.

A veterinary medicine for preventing and treating the frequently encountered diseases of pig, such as metritis, mammitis, acyesis, sterility, dysentery, epidemic enteritis, paratyphoid, asthma, etc is prepared from 15 raw materials including florfenicol, amoxicillin, lucomycin, doxycycline, etc.

The invention relates to a purification method for tylosin. The invention describes the post treatment of the extraction liquid of tylosin when chloroform is used for extracting the tylosin from fermentation filter liquid, i.e. a purification method. The essential of the method is as follows: the extraction liquid is concentrated into a condition that chloroform is basically removed; a condensateis dissolved by acid water; butyl acetate or toluene or 4-methyl-2-pentanone is added; the pH value is adjusted to 7.5-9.5 by alkali liquid, and extraction and phase splitting are sequentially performed; and a tartaric acid aqueous solution or a phosphoric acid aqueous solution is added to an ester phase for performing back extraction, back extraction liquid is obtained and is sprayed and dried, or the pH value of the back extraction liquid is adjusted to 7.5-10.5, and the back extraction liquid is pressed and filtered or centrifugated to obtain the tylosin.

Embodiments provided herein relate to compositions, methods, and devices useful for treating eye stain. More particularly, compositions and devices are provided that include tylosin and a carrier suitable for ophthalmic application. Such compositions and devices can be used to treat eye stain in an animal, such as a dog or cat.

The invention relates to a culture medium for producing acetylisovaleryltylosin by fermenting streptomyces thermotolerans and a fermentation method. According to the culture medium and the fermentation method, a culture medium formula and a production technology are confirmed, and stable and high-efficiency production of acetylisovaleryltylosin is realized. In addition, the culture medium and the production technology can raise a fermentation unit, shorten the fermentation period, and increase the yield of acetylisovaleryltylosin.

Use of a macrolide antibiotic for the manufacture of a medicament for the treatment or prevention of a cancer selected from colorectal cancer, Desmoid tumor, bladder cancer, gastric cancer, and breast cancer, the macrolide antibiotic being one or more of : tylosin, pharmaceutically acceptable salts thereof, and derivatives thereof; erythromycin, pharmaceutically acceptable salts thereof, and derivatives thereof; oleandomycin, pharmaceutically acceptable salts thereof, and derivatives thereof; and spiramycin, pharmaceutically acceptable salts thereof, and derivatives thereof. Also provided are pharmaceutical compositions and methods for the treatment or prevention of the above mentioned cancers and methods for treating or preventing, in a mammal, a cancer that expresses a mutated APC gene.

The invention relates to the field of comprehensive utilization of tylosin residues and waste water of vitamin B12, and provides a method which is to use the tylosin residues and the waste water of the vitamin B12 as raw materials, separate and extract protoplasm substances in a mycelium to prepare a thallus extract through microbial fermentation treatment, and apply the thallus extract in a biological medium. The thallus extract and the preparation method thereof open a new path for utilization of the tylosin residues and the waste water of the vitamin B12, and provide possibility of realizing clean production which is a goal of sustainable development for the fermentation industry of the tylosin and the vitamin B12. Furthermore, the method solves the problem that the tylosin residues and the waste water of the vitamin B12 pollute environment and are harmful to human health.

A method for extracting tylosin by tylosin fermentation broth is characterized by including steps of firstly pretreating the tylosin fermentation broth to obtain tylosin filtrate, then subjecting the tylosin filtrate to extracting, reextracting, decolorizing filtering, crystallizing, ageing, rinsing and vacuum drying to obtain the tylosin. The method is used for directly extracting the tylosin by the tylosin fermentation broth serving as raw materials, tylosin solubility of intermediates of the tylosin accords with quality standards of Chinese Veterinary Pharmacopoeia. The integral method is mild in production conditions, simple to operate, safe and reliable in preparation process and low in loss of organic solvent and has the advantages of energy conservation, consumption reduction, low production cost, high work efficiency and the like.

The invention relates to an improved extraction method for tylosin. The invention describes the process of using a cosolvent to extract the tylosin from tylosin fermentation filter liquid. The cosolvent comprises chloroform or methylene dichloride and ethyl acetate or butyl acetate or amyl acetic ester or isoamyl acetate or 4-methyl-2-pentanone or toluene. The method using the cosolvent to extract the the tylosin from the fermentation filter liquid has the advantages that the use quantity of the cosolvent is only 1-8% of the quantity of the fermentation filter liquid; the extraction rate is high and can reach 98.8%; and the cosolvent has a selective extraction function, the impurity content of extraction liquid is very little, product purification is convenient and the product purity is improved.

The present invention discloses a method for detecting tylosin and its special-purpose ELIA kit. Said kit includes tylosin specific antibody, coating source and enzyme label. The described coating source is conjugate of tylosin semiantigen and carrier protein or antiantibody, and the described enzyme label is enzyme-labelled antiantibody or enzyme-labelled tylosin semiantigen. When the described coating source is the conjugate of tylasin semiantigen and carrier protein, the described enzyme label is enzyme-labelled antiantibody, and when the described coating source is antiantibody, the described enzyme lable is enzyme-labelled tylosin semiantigen.

The invention discloses an acetylisovaleryl tylosin nanoemulsion anti-mycoplasma drug. The diameter of the nanoemulsion droplets is between 1 and 100 nm, and is composed of the following raw materials in mass percentage: 15.00% to 36.00% of surfactant, Co-surfactant 2.00%-20.00%, oil 1.00%-5.00%, acetylisovaleryl tylosin 0.01%-2.00%, the balance is distilled water, and the sum of the mass percentages of the above raw materials is 100%. The invention improves the drug stability of the acetylisovaleryl tylosin, improves the bioavailability of the acetylisovaleryl tylosin, delays its drug metabolism time in the body, reduces the adjuvant dosage, reduces the production cost, and is used in medicine. The field has broad market prospects.

The invention relates to a method for making use of tylosin dregs of decoction, in particular to a method for producing a feed composite enzyme preparation by making use of the tylosin dregs. The method comprises the steps of solid fermentation, drying, and crushing to obtain the feed composite enzyme preparation. The method can degrade residual tylosin in the dregs while the feed composite enzyme preparation is produced through fermentation by the method; the existence of the residual tylosin in the feed composite enzyme preparation is not detected through a microbiological method; and strains used in the production of the feed complex enzyme preparation are aspergillus niger strains and degrading bacteria for degrading the tylosin in the dregs.

The invention relates to a soluble powder for treating poultry respiratory tract infection, comprising tilmicosin, sulfadimidine sodium and auxiliary materials, the medicament of the invention is taken as antibiotic series medicament and mainly used for treating various respiratory infection caused by sensitive bacteria and poultry chronic respiratory disease, the invention combines the sulfadimidine sodium with the tilmicosin for using and acting on different sensitive bacteria simultaneously, solves the problem of generating medicament resistance after singly using the tylosin to treat the disease, the invention has good oral administration absorption effect and high safety.

The invention relates to a compound menstruum for extracting tylosin and an extraction method. According to the invention, the compound menstruum formed by mixing butyl acetate, isobutyl acetate and butanol according to the volume ratio of 6:3 to 3.2 to 0.8-1 is used for extracting tylosin in fermentation filtrate through a secondary counter-current extraction method. The method has the advantages of low consumption of organic menstruum, low loss of tylosin, short extraction time, high extraction yield and the like.

The invention relates to a preparation method of crystalline particles of acetyl isovaleryl tylosin salt. The method comprises the steps of sufficiently dissolving powder of the acetyl isovaleryl tylosin salt in ethyl acetate, slowly and dropwise adding isobutanol for mixing in a stirring state and at 0-2 DEG C, standing after the mixing, conducting solid-liquid separation, eluting, and conducting vacuum drying. According to the preparation method, the acetyl isovaleryl tylosin salt is prepared into the crystalline particles to replace powder; the specific gravity of the crystalline particles is greater, so that dust pollution is avoided in packaging and using processes; the crystalline particles are added to animal drinking water to sink into the water bottom, are better in dispersity, higher in dissolving speed and higher in content, and can be directly added to animal feed or the animal drinking water; a used organic solvent can be recovered and recycled; and the preparation method has a certain application prospect.

The invention relates to Streptomyces hawaii. The microorganism can be used for producing acetylisovaleryl tylosin through fermentation, and the fermentation time is short. Tylosin is supplemented by using a continuous flow, subtract inhibition is reduced, a forward reaction is promoted, and thus, the efficacy of AIV (Avian Influenza Virus) is improved. The fermentation time is 5 days and is shortened by 2 days as compared with the traditional production process. The preservation name of Streptomyces hawaii provided by the invention is Streptomyces hawaiiensis 0915, the preservation unit is the China Center for Type Culture Collection, the preservation date is October 16th, 2010, and the preservation number is CCTCC NO: M2010261. In the fermentation process, the fermentation period is 5 days no matter for flask shaking fermentation or for various tank fermentations and is shortened by 2 days as compared with the fermentation time of reported Streptomyces thermotolerans E1 strains. The efficacy of Streptomyces hawaiiensis after fermentation 0915 CCTCC NO: M2010261 is 18.6 mg / ml, and the transformation efficiency is as high as 90% and is obviously higher than that of the reported Streptomyces thermotolerans.

The invention discloses tylosin degrading bacteria and application thereof, and belongs to the technical field of microorganism degrading antibiotics residues. The tylosin degrading bacteria TYL2 is brevibacillus borstelensis TYL2 and is preserved in the common microorganism center of the China microorganism culture preservation management council, wherein the preservation date is 29 / 12 / 2018, thepreservation serial number is CGMCC No.17051, and the preservation address is No.3 microorganism research laboratory of Chinese Academy of Sciences in No.1 yard at the Beichen west road of Chaoyang district of Beijing. With drug residues being primary raw materials for sieving, an obtained strain and a prepared fungicide can be directly used for treating waste of a tylosin enterprise, and can be also used for repairing surrounding soil and a water body environment which are polluted by tylosin.

The invention discloses a refining method of tylosin. The refining method comprises the following steps: (1) dissolving coarse tylosin in an organic solvent, then adding active carbon, stirring to carry out a decoloring treatment so as to obtain a mixture; (2) filtering the mixture, namely orderly subjecting the mixture to microfiltration, ultra-filtration, and nano-filtration, then condensing the filtrate to obtain a condensed liquid; (3) spray-drying the obtained condensed liquid so as to obtain the tylosin. The provided refining method is capable of improving the purity of coarse tylosin to 99% or more by combining organic dissolution, active carbon decoloring, and multiple membrane filtration technologies. The pyrogen in tylosin is completely removed, the processed tylosin reaches the injection grade, and the yield can reach 93% to 96%. Moreover, during the refining process, no other substance is added, the probability of secondary impurity pollution is avoided, the nano-filtration can play a condensing function, the reutilization rate is high, the energy consumption during the drying process is greatly reduced, the technology is simple, the operation is convenient, and the refining method is suitable for massive industrial production.

The invention discloses a method for preparing organic fertilizer from tylosin dregs, relates to a method for harmless treatment and resourceful utilization of tylosin dregs and aims to solve the problems that existing tylosin dregs are high in treatment cost, environmental risks still exist after treatment and the like. Tylosin dregs are mixed with persulfate and copper sulfate, the mixture is heated in a water bath, oxidized and centrifuged, the mushroom dregs are collected, and the organic fertilizer is prepared. The removal efficiency of persulfate for tylosin dregs is up to 99.9%, the sterilization capability is high, the treatment cycle is short, and treatment can be completed within 0.5-1.0 h.

The invention relates to a veterinary compound terramycin injection and a preparation method thereof. The injection comprises the following raw materials: terramycin, tylosin, sodium camphorsulfonate,organic solvent and water for injection. The veterinary compound terramycin injection is used as a special veterinary compound preparation, has especially good effect for curing respiratory diseasesof poultry and livestock caused by the bacteria and mycoplasma infection and has the advantage of easy operation, good stability, short treatment period, low drug resistance and the like.

The invention discloses tylosin hapten, artificial antigen and antibody as well as preparation methods and application thereof. Specifically, a glyoxyl functional group of a large ring side chain of tylosin is used as a derived coupling site; formyl is converted into carboxyl through a one-step oxidization method, so as to obtain the tylosin hapten with a carboxymethylated coupling arm. The obtained hapten and a framework structure have a high overlapping degree and a foundation is laid for exposure of a characteristic structure of the tylosin and production of a high-specificity antibody. Furthermore, the hapten and carrier protein are coupled to obtain the artificial antigen and an animal is immunized to obtain the tylosin high-specificity antibody; an established enzyme linked immunosorbent assay has high accuracy and good sensitivity; the detection limit reaches 0.051ng / mL and the half inhibitory concentration is 1.39ng / mL; the enzyme linked immunosorbent assay is simple to operateand low in cost, is very suitable for field detection, can be used for rapidly and effectively detecting the tylosin in environment and food samples and has a wide application prospect and economic value.

The invention discloses a hapten, an artificial antigen and a monoclonal antibody for tylosin and preparation methods and applications thereof. The hapten contains a common structural part of tylosin, tilmicosin, acetyl-isovaleryl tylosin and a tylosin metabolin, i.e., cladinose-removed tylosin; and in the preparation process of a tylosin artificial antigen, introduced carboxyl is connected with a carrier protein, i.e., the common structural part is kept far away from the carrier protein and is fully exposed to an immunological recognition of animals. A broad-spectrum specific antibody which can be used for recognizing the tylosin, tilmicosin, the acetyl-isovaleryl tylosin and the tylosin metabolin, i.e., cladinose-removed tylosin simultaneously can be obtained by taking the artificial antigen as an immunogen immune animal. When the broad-spectrum specific antibody is used for rapidly immunologically analyzing and sieving, the detection efficiency is increased, the detection time is shortened, and the detection cost is lowered.

The invention relates to a veterinary compound enrofloxacin injection and a preparation method thereof. The veterinary compound enrofloxacin injection mainly comprises enrofloxacin, sulfamethoxazole,trimethoprim, flunixin meglumine, tylosin, organic solvent and water for injection. The veterinary compound enrofloxacin injection is used as a veterinary special compound preparation, used for treating various infectious diseases of a respiratory system, a digestive system and a skin soft tissue caused by livestock and poultry bacterial and mycoplasma infection, particularly has special effects on secondary hemophilus disease of pigs and poultries caused by hemophilus and has the advantages of convenient use, short course, low drug resistance and the like.

The invention relates to the fields of analytical chemistry and food safety, and provides a detection method of the residual amount of a plurality of macrolide veterinary drugs in casings. The detection method comprises the following steps of: extracting a sample with methanol or acetonitrile; purifying the obtained extracting solution by a C18 or Oasis HLB (hydrophilic-lipophilic balance) solid-phase extraction column, taking an acetonitrile-methanoic acid aqueous solution with the concentration of 0.15% as a mobile phase, and performing gradient elution by adopting a ZORBAX Eclipse C8 analytical column; and performing electro-spraying, separating by a cationic scanning mode and detecting the residual amount of seven types of macrolide veterinary drugs. The detection lower limit of the seven types of macrolide veterinary drugs is 20 mu g / kg. The recovery rates of spiramycin, tilmicosin, oleandomycin, tylosin, erythrocin, roxithromycin and josamycin are respectively 71.4-78.8, 75.7-85.6, 81.1-85.8, 78.9-83.0, 87.1-89.6, 83.7-85.4 and 75.6-81.8; and the indoor relative standard deviations for the recovery rates of the spiramycin, the tilmicosin, the oleandomycin, the tylosin, the erythrocin, the roxithromycin and the josamycin are respectively 7.7-11.1, 8.0-15.7, 10.5-16.4, 8.1-12.5, 10.5-12.4, 10.6-14.4 and 7.8-18.0. The detection limit, the recovery rate, the precision and the like meet the related requirements at home and abroad.