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Method for producing complex enzyme preparation for feeding by using tylosin herb residue

A feed compound enzyme and tylosin technology, which is applied in the direction of microorganism-based methods, biochemical equipment and methods, enzymes, etc., can solve the problems of high price, high production cost of enzyme preparations, and difficulty in achieving practical and affordable applications, etc. problems, to achieve low production costs, increase product competitiveness, and good environmental protection effects

Inactive Publication Date: 2009-05-06
NINGXIA UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the raw materials for the production of feed enzyme preparations are mainly soybeans, corn, etc., and the price is relatively high, the production cost of enzyme preparations is still at a relatively high level, and it is difficult to achieve practical and affordable applications.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 1) Aspergillus niger is inserted into the Aspergillus niger rejuvenation medium, after the bacterial strain is activated and expanded, it is inoculated on the Aspergillus niger seed medium with an inoculum size of 5%, and after cultivating at 32°C for 120h, the compound enzyme preparation for production of feed is obtained. Aspergillus niger seeds.

[0027] 2) Insert the degrading bacteria that degrade the dregs of tylosin into the degrading bacteria rejuvenation medium, after the degrading bacteria are activated and expanded, inoculate the first-level degrading bacteria seed medium with 5% inoculum, and cultivate at 32°C for 24 hours Then transfer to the secondary degrading bacteria seed culture medium, cultivate at 32°C for 30 hours, then transfer to the third-level degrading bacteria seed medium, and after culturing at 32°C for 38 hours, obtain the degrading bacteria seeds for degrading the dregs of tylosin.

[0028] 3) The Aspergillus niger seeds prepared in the abo...

Embodiment 2

[0034] 1) Aspergillus niger is inserted into the Aspergillus niger rejuvenation medium, after the bacterial strain is activated and expanded, it is inoculated in the Aspergillus niger seed medium with an inoculum size of 10%, and after cultivating at 32°C for 72h, the compound enzyme preparation for production of feed is obtained. Aspergillus niger seeds.

[0035] 2) Insert the degrading bacteria that degrade the dregs of tylosin into the degrading bacteria rejuvenation medium, after the degrading bacteria are activated and expanded, inoculate the first-level degrading bacteria seed medium with 10% inoculum, and cultivate at 32°C for 18 hours Then transfer to the secondary degrading bacteria seed culture medium, cultivate at 32°C for 26 hours, then transfer to the third-level degrading bacteria seed medium, cultivate at 32°C for 34 hours, and obtain the degrading bacteria seeds for degrading the dregs of tylosin.

[0036]3) The Aspergillus niger seeds for the production of the...

Embodiment 3

[0042] 1) Aspergillus niger is inserted into the Aspergillus niger rejuvenation medium, after the bacterial strain is activated and expanded, it is inoculated in the Aspergillus niger seed medium with an inoculum size of 8%, and after cultivating at 30°C for 84h, the compound enzyme preparation for production of feed is obtained. Aspergillus niger seeds.

[0043] 2) Insert the degrading bacteria that degrade the dregs of tylosin into the degrading bacteria rejuvenation medium, after the degrading bacteria are activated and expanded, inoculate the first-level degrading bacteria seed medium with an inoculum of 8%, and cultivate at 30°C for 22 hours Then transfer to the secondary degrading bacteria seed culture medium, cultivate at 30°C for 28 hours, then transfer to the third-level degrading bacteria seed medium, and after culturing at 30°C for 36 hours, obtain the degrading bacterial seeds for degrading the dregs of tylosin.

[0044] 3) The Aspergillus niger seeds for the produ...

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PUM

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Abstract

The invention relates to a method for making use of tylosin dregs of decoction, in particular to a method for producing a feed composite enzyme preparation by making use of the tylosin dregs. The method comprises the steps of solid fermentation, drying, and crushing to obtain the feed composite enzyme preparation. The method can degrade residual tylosin in the dregs while the feed composite enzyme preparation is produced through fermentation by the method; the existence of the residual tylosin in the feed composite enzyme preparation is not detected through a microbiological method; and strains used in the production of the feed complex enzyme preparation are aspergillus niger strains and degrading bacteria for degrading the tylosin in the dregs.

Description

technical field [0001] The invention relates to a method for utilizing tylosin dregs, in particular to a method for producing compound enzyme preparation for feed by utilizing tylosin dregs. Background technique [0002] China is the largest producer of macrolide antibiotics, with annual output exceeding 2 / 3 of the world's total. However, in the process of antibiotic production, a large amount of waste medicine dregs will be produced. These medicine dregs are mainly the bacteria cake formed during the solid-liquid separation of the fermented mash. The main components are microbial mycelium, unmetabolized organic matter, Inorganic salts, a small amount of unseparated antibiotics and their degradation products. For a long time, antibiotic dregs are mostly used as animal feed or fertilizer after simply drying or drying, with low economic added value. At the same time, due to the presence of unseparated antibiotics in the dregs, its direct use as feed or fertilizer has been qu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/42C12N9/26C12N9/50C12P39/00A23K1/165C12R1/685C12R1/01A23K20/189
Inventor 马玉龙张作义刘宁普谢丽
Owner NINGXIA UNIVERSITY
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