19 results about "Antibiotic production" patented technology

The present invention relates to the technical field of antibiotic production, and in particular to a dalbavancin intermediate A40926 and a separation and purification method thereof, comprising the following steps: S1, filtering a fermentation broth containing the dalbavancin intermediate A40926 to obtain a collected liquid; S2, adsorbing the collected liquid through a macroporous adsorption resin, eluting with ethanol, and collecting an eluate a having an A40926 unit greater than 100 mg / L; S3, ultrafiltrating and concentrating the eluate a to obtain a concentrated liquid having an A40926 unit of 15,000 to 16,000 mg / L; S4, subjecting the concentrated liquid to a chromatography exchange resin, top-washing with pure water, and gradient eluting with an ammonium acetate-ethanol solution, to collect an eluate b having an A40926 purity greater than 95%; S5, adjusting the pH of the eluate b to 8.0±0.2, adding acetone, adjusting the feed liquid temperature to 2 to 8°C, stirring for 6 to 7 hours, performing crystallization, and filtering and drying to obtain the dalbavancin intermediate A40926.

This invention belongs to the field of pharmaceutical wastewater treatment technology, and particularly relates to an ultrasonic-coupled electrochemical pretreatment device and method for high-concentration antibiotic production wastewater. The device consists of an ultrasonic treatment first reactor, an electrochemical second reactor, and a flocculation sedimentation third reactor connected in series via pipelines. The first reactor is equipped with a wall-mounted ultrasonic transducer; the second reactor contains a stabilizing electrode and a sacrificial electrode, and multiple disc aerators are installed at the bottom; the third reactor contains an overflow trough, inclined tube packing, and an electromagnet. This invention can achieve efficient desorption and mineralization degradation of adsorbed antibiotics in suspended solids in production wastewater, reduce antibiotic and degradation product residues in flocs, and eliminate toxic effects on subsequent biological treatment processes.

The utility model discloses a high-salt antibiotic wastewater treatment device which comprises a DT membrane filtering assembly, a forced crystallization assembly and a crystallized salt separating and drying assembly which are sequentially communicated, the DT membrane filtering assembly is used for filtering and separating wastewater, and the forced crystallization assembly is used for concentrating and crystallizing wastewater. The crystallized salt separating and drying assembly is used for separating and drying crystallized salt, and a salt outlet pipeline of the crystallized salt separating and drying assembly is communicated with the crystallized salt temporary storage device. According to the high-salt antibiotic wastewater treatment device disclosed by the utility model, a process of synergistically combining the DT membrane filtering component, the forced crystallization component and the crystallized salt separating and drying component is adopted, so that sodium chloride in antibiotic wastewater can be continuously, efficiently and quickly separated and purified; the method has the advantages of simple operation process, strong operability and controllability, high recovery rate, and capability of obtaining sodium chloride meeting the standard of being reused for an antibiotic production line, meets the requirements of production and reuse, is energy-saving and environment-friendly, and reduces the operation cost.

The application provides an antibiotic generation method based on antibacterial activity guided search optimization, and the implementation steps are as follows: (1) obtaining an antibiotic molecule data set; (2) constructing a training sample set and a test sample set; (3) constructing an antibacterial activity prediction network model C; (4) iteratively training the antibacterial activity prediction network model C; (5) constructing an antibiotic generation network model H; (6) iteratively training the antibiotic generation network model H; (7) optimizing the antibiotic molecule through antibacterial activity guided search; and (8) generating an optimization result; the application solves the technical problems of low generation efficiency and inability to guarantee that the generated compound molecules after optimization all have expected activity in the prior art, effectively improves the generation efficiency, and guarantees that the generated antibiotic molecules all have expected antibacterial activity, and can be used for guiding the research and development of new drugs.

The invention belongs to the technical field of antibiotic production, and particularly relates to a method for increasing the yield of an aureomycin premix. The method comprises the following steps: sampling aureomycin fermentation liquor, detecting the titer of the fermentation liquor and the dry matter of the fermentation liquor, calculating the use amount of calcium carbonate according to the preparation specification of the aureomycin premix to be produced, the volume of the fermentation liquor and the actual yield value, and adding calcium hydrophosphate and ethoxyquin according to the corresponding proportion to obtain the aureomycin calcium salt complex. And carrying out plate frame filtration and flash evaporation drying to obtain the aureomycin premix. According to the invention, the stability of the aureomycin calcium salt is effectively improved, and the yield of the aureomycin premix is improved. The method is simple in technological process, controllable in operation, stable in product quality and low in production cost.

The invention belongs to the technical field of wastewater and solid waste treatment and resource utilization, and particularly relates to a co-hydrothermal resource treatment method for chlorine-containing ammonia nitrogen wastewater and antibiotic mushroom dregs. The method comprises the following treatment steps: uniformly stirring and mixing the antibiotic mushroom dregs and the chlorine-containing ammonia nitrogen wastewater, and adjusting the pH value to 0.2-0.6 by using an acid solution; feeding the mixed bacterial liquid obtained after pretreatment into a hydrothermal reaction kettle, carrying out hydrothermal reaction at 150-200 DEG C, controlling the pressure to be 0.5-1.0 Mpa through a pressure stabilizing system, and after the reaction is completed, cooling, relieving the pressure and discharging; and carrying out solid-liquid separation on the mixed solution obtained by the hydrothermal reaction to obtain reduced and harmless micromolecule complex liquid and solid slag. According to the method disclosed by the invention, synchronous reduction, harmless and resourceful treatment of the high-ammonia-nitrogen wastewater and the antibiotic mushroom dregs in the antibiotic production process is synergistically realized.

The invention relates to a recycling treatment method of cephalosporin antibiotic production wastewater. The method comprises a wastewater flocculation treatment step, a hollow fiber membrane ultrafiltration treatment step, a step of carrying out concentration treatment on ultrafiltration produced water by adopting a reverse osmosis membrane, a step of carrying out salt separation treatment on reverse osmosis concentrated water by adopting a nanofiltration membrane, and a step of carrying out concentration treatment on nanofiltration produced water by adopting a reverse osmosis membrane. According to the method, water and salt (monovalent salt and divalent salt) are effectively recycled from the cephalosporin antibiotic production wastewater, the recycled water can be used as water for circulating cooling water, and the water quality of the water meets related requirements of the water for circulating cooling water of enterprises, so that the emission of pollutants is reduced, and even zero emission of sewage is realized; and the method has remarkable social benefits and economic benefits.

The application discloses application of Pseudomonas pseudoalcaligenes 94-6Y in removing pollutants in wastewater, and belongs to the technical field of microorganisms. The Pseudomonas pseudoalcaligenes 94-6Y has the preservation number of CGMCC No.32718, has the characteristic of tolerating penicillin, can grow in penicillin wastewater with the concentration of 0.1-2.5 mg / L, and the effective viable cell count can reach 5x10 8 CFU / mL after 72 hours in the penicillin-containing wastewater, and it is also proved that the Pseudomonas pseudoalcaligenes 94-6Y has the characteristic of efficiently reducing pollutants in wastewater, wherein the removal rate of chemical oxygen demand reaches 68.41%, the removal rate of ammonia nitrogen reaches 85.92%, and the removal rate of total phosphorus reaches 21.3%, which has a significant effect on improving the water quality of tail water of antibiotic production industry and reaching the discharge standard.

A transporter protein T6417 and its application in improving the yield of Streptomyces natural products belong to the field of genetic engineering technology. In order to improve the yield of Streptomyces natural products, based on the analysis of the transcriptome data of Streptomyces caniferus NEAU6, this invention screened transporter proteins related to yield and the timing of antibiotic production, and obtained the candidate transporter protein T6417 through functional annotation analysis; then the expression of T6417 was adapted using different promoters, and it was found that overexpressing the transporter protein T6417 in Streptomyces caniferus, Streptomyces bingchenggensis or Streptomyces avermitilis can improve the yield of natural products. This invention provides valuable biosynthetic elements for the efficient biomanufacture of Streptomyces.

This invention belongs to the field of wastewater and solid waste treatment and resource utilization technology, specifically relating to a hydrothermal resource utilization method for treating chlorinated ammonia nitrogen-containing wastewater and antibiotic bacterial residue. The method includes the following steps: antibiotic bacterial residue and chlorinated ammonia nitrogen-containing wastewater are stirred and mixed evenly, and the pH is adjusted to 0.2–0.6 with an acidic solution; the pretreated mixed bacterial solution is fed into a hydrothermal reactor and subjected to a hydrothermal reaction at 150–200°C, with the pressure controlled at 0.5–1.0 MPa by a pressure stabilization system; after the reaction is completed, the mixture is cooled, depressurized, and discharged; the mixture obtained from the hydrothermal reaction is subjected to solid-liquid separation to obtain a reduced-volume, harmless small-molecule composite liquid and solid residue. This invention synergistically achieves the simultaneous reduction, harmlessness, and resource utilization of high-ammonia nitrogen wastewater and antibiotic bacterial residue during antibiotic production.

The invention relates to a method of modulating production of a metabolite in an actinomycetes bacterium comprising increasing the expression or activity of the Sporulation and Antibiotic Production Related gene A (SarA. SCO4069) polypeptide to modulate production of the metabolite in the actinomycetes bacterium. In one embodiment, the metabolite is valinomycin, daidzein, fluvirucin, demethyllydicamycin, or TPU-0037 analogues A. C and D.

The application relates to the technical field of functional egg production management, and discloses a blockchain traceability method and system for non-antibiotic production of functional eggs, which records multi-source traceability data of functional eggs from breeding to the whole chain of sales, generates a hash value and a digital identity identifier by using the multi-source traceability data, associates the digital identity identifier with the multi-source traceability data, binds the hash value with the digital identity identifier by using a blockchain, generates a two-dimensional code picture through the digital identity identifier, and traces the source by scanning the two-dimensional code. The multi-source traceability data of functional eggs from breeding to the whole chain of sales is recorded and collected, information is more comprehensive, the hash value and the digital identity identifier are generated by using the multi-source traceability data and are associated, the data can be prevented from being modified by setting the hash value, the data authenticity is improved, the data can be better mastered, and all the information is recorded by using the regional chain, so that information islands are avoided.

The invention relates to the technical field of antibiotic production, in particular to a production process of an aureomycin premix. The invention provides a production process of an aureomycin premix, which comprises the following specific steps: adding aureomycin fermentation liquor into a composite carrier, mixing, and carrying out plate frame filtration to obtain a filter cake with the water content of 50%; crushing the filter cake to obtain a wet material, adding aureomycin powder into the wet material, and reducing the water content to be less than 40%; adding an adhesive into the wet material and uniformly mixing; granulating the wet material by using a granulator, and then collecting a wet granular material; and drying the wet granulated material in a fluidized bed, and screening the dried material to obtain a 20-80-mesh material. By optimizing the process, the particle yield of the aureomycin premix is remarkably improved, meanwhile, powder residues are greatly reduced, efficient recycling of the screened powder is achieved, and raw material loss is reduced.

The invention discloses a volatile organic compound adsorption and purification method in antibiotic drug production, and belongs to the technical field of pharmaceutical chemical waste gas treatment. The method comprises the following steps: (1) introducing the antibiotic production waste gas into a pretreatment unit, treating by using an alkali liquor spray tower, and condensing and dehumidifying to enable the dew point to be less than or equal to 8 DEG C; (2) introducing the pretreated waste gas into a three-stage adsorption system at a flow rate of 0.8-1.2 m / s to adsorb xylene and sulfides in the waste gas; and (3) when the VOCs concentration at an outlet is greater than or equal to 30mg / m < 3 > or the sulfide concentration at a secondary adsorption inlet is greater than or equal to 1ppm through continuous twice detection, starting regeneration operation: regenerating first-stage silica gel by using hot air at 110-120 DEG C, regenerating second-stage activated carbon by using nitrogen at 120-130 DEG C, regenerating a third-stage molecular sieve by using superheated steam at 130-150 DEG C, and returning regenerated tail gas to an inlet of an alkali liquor spray tower of the pretreatment unit. According to the invention, efficient gradient adsorption of multi-component VOCs is designed, the dynamic capacity fluctuation can be reduced, the service life of the adsorbent is prolonged, the attenuation rate is lower than 5% per cycle, and the regeneration energy consumption is obviously reduced by more than 35%.

The present invention discloses a method for adsorption and purification of volatile organic compounds in the production of antibiotic drugs, which belongs to the technical field of pharmaceutical and chemical waste gas treatment. The method comprises: (1) passing the waste gas from the production of antibiotics into a pretreatment unit, treating it with an alkaline solution spray tower, and then dehumidifying it by condensation to make the dew point ≤8°C; (2) passing the pretreated waste gas into a three-stage adsorption system at a flow rate of 0.8 to 1.2 m / s to adsorb xylene and sulfide in the waste gas; (3) when the outlet VOCs concentration is ≥30 mg / m 3 If the sulfide concentration at the secondary adsorption inlet is ≥1 ppm for two consecutive measurements, regeneration is initiated: the primary silica gel is regenerated with 110-120°C hot air, the secondary activated carbon is regenerated with 120-130°C nitrogen, and the tertiary molecular sieve is regenerated with 130-150°C superheated steam. The regenerated exhaust gas is returned to the inlet of the alkali spray tower in the pretreatment unit. This invention designs a multi-component VOCs high-efficiency gradient adsorption system that reduces dynamic capacity fluctuations, extends adsorbent life, and achieves a decay rate of less than 5% per cycle, significantly reducing regeneration energy consumption by over 35%.

The invention provides a flash evaporation drying method of salinomycin fermentation liquor, and belongs to the technical field of antibiotic production. The preparation method comprises the following steps: preheating salinomycin fermentation liquor, and mixing the preheated salinomycin fermentation liquor with a flocculating agent, diatomite and white carbon black to obtain dispersion liquid; carrying out plate frame filtration on the dispersion liquid to obtain wet mycelia; and carrying out flash evaporation drying on the wet mycelium. After the salinomycin fermentation liquor is preheated, the salinomycin fermentation liquor is mixed with the flocculating agent, the kieselguhr and the white carbon black, and the flocculating agent and the kieselguhr can effectively improve the plate frame filtering speed of the salinomycin fermentation liquor; the salinomycin fermentation liquor contains residual oil (including soybean oil and the content of the residual oil in the salinomycin fermentation liquor is 2-8wt%), and materials are easy to accumulate during flash evaporation, the white carbon black is added, so that the residual oil can be effectively adsorbed, the problems of material accumulation and wall sticking during flash evaporation can be solved, the salinomycin flash evaporation powder with qualified quality can be obtained, and the salinomycin yield can be increased.

This invention relates to the field of antibiotic production separation and purification, specifically to a method for vancomycin crystallization with calcium addition, comprising the following steps: dissolving crude vancomycin powder and adjusting the pH to 2.0-4.0 to obtain a solution; filtering the obtained solution through a membrane and adjusting the pH to 2.0-4.0 to obtain a pre-crystallization solution; adding a calcium salt solution to the pre-crystallization solution, then adding ethanol, allowing it to stand, and filtering to obtain crystalline powder; dissolving the obtained crystalline powder in deionized water, removing ethanol using a small membrane apparatus until it meets the requirements, and then obtaining a removed ethanol solution; lyophilizing the obtained removed ethanol solution to obtain the desired vancomycin finished powder. The beneficial effects of this invention are: improving the existing process of crystallization under alkaline conditions, improving the flocculation of vancomycin by using acidic crystallization and the addition of calcium ions, utilizing the effect of ethanol on flocculation for crystallization, and finally lyophilizing the obtained crystalline powder to obtain the required vancomycin finished powder, thus extending the flocculation time of 25% concentration vancomycin.

The invention discloses application of pseudobacillus Australii 94-6Y in removal of pollutants in wastewater, and belongs to the technical field of microorganisms. The preservation number of the pseudomonas australis is CGMCC No.32718, the pseudomonas australis 94-6Y has the characteristic of penicillin tolerance and can grow in penicillin wastewater with the concentration of 0.1-2.5 mg / L, the effective viable count of the pseudomonas australis 94-6Y can reach 5 * 10 < 8 > CFU / mL after the pseudomonas australis 94-6Y is put in the penicillin-containing wastewater for 72 hours, and the pseudomonas australis 94-6Y has the characteristic of penicillin tolerance and can grow in the penicillin wastewater with the concentration of 0.1-2.5 mg / L. Meanwhile, it is also proved that the pseudobacillus Australiae 94-6Y has the characteristic of efficiently reducing pollutants in wastewater, the chemical oxygen demand removal rate reaches 68.41%, the ammonia nitrogen removal rate reaches 85.92%, the total phosphorus removal rate reaches 21.3%, and the pseudobacillus Australiae 94-6Y has remarkable effects on improving the quality of tail water of the antibiotic production industry and achieving the emission standard.

The invention belongs to the technical field of genetic engineering, and discloses a genetic engineering strain and method for improving the fermentation yield and conversion rate of L-glutamine and application of the genetic engineering strain and method for improving the fermentation yield and conversion rate of the L-glutamine. A glutamate transporter gene cgl1221 is deleted, and a natural promoter of an alpha-ketoglutarate dehydrogenase gene cgl1129 is replaced by a growth coupled promoter PCP-2836, so that the gene cgl1221 is constructed. By reducing the secretion of the by-product L-glutamic acid and dynamically regulating and controlling the expression level of the key metabolic node alpha-ketoglutarate dehydrogenase, the yield of the L-glutamine and the sugar acid conversion rate are improved. The finally obtained engineering bacteria do not need to carry plasmids, antibiotics do not need to be added in the fermentation process, the production stability is higher, and the application is wider.