16 results about "Chromatography liquid" patented technology

The invention discloses a marine-derived bioactive peptide with a blood sugar reducing effect, a preparation and application, and belongs to the technical field of bioactive peptides. The amino acid sequence of the bioactive peptide is at least one of FYP, FPF and YAPFPK. Three bioactive peptides with the blood sugar reducing effect are separated and purified from oysters through enzymolysis, ultrafiltration, gel chromatography, liquid chromatography-mass spectrometry and bioinformatics technologies. According to the present invention, chemical synthesis is performed according to the identified peptide fragment sequence, the hypoglycemic activity of the synthesized single peptide fragment and the compounded peptide fragment is detected, and the bioactive peptides have good alpha-glucosidase inhibition activity, such that the bioactive peptides have good application prospects in the preparation of the products with the hypoglycemic effect, and can be widely used in the preparation of the products with the hypoglycemic effect. And a foundation is laid for high value-added utilization of oysters and promotion of research, development and application of functional active peptides.

The utility model provides an environment-friendly row type pigment chromatography device which comprises chromatography tubes, a flow divider and an injector, the chromatography tubes are used for loading a stationary phase, the bottoms of the multiple chromatography tubes are communicated with the flow divider so that the multiple chromatography tubes can be connected to the flow divider in parallel, the injector is used for loading chromatography liquid, and the flow divider is communicated with the flow divider so that the multiple chromatography tubes can be connected to the flow divider in parallel. And the output end of the injector is communicated with the flow divider. According to the invention, multiple groups of pigment chromatography experiments can be completed at one time through the multiple parallel chromatography tubes, and multiple samples can be treated at the same time, so that time and resources are saved, and the experiment efficiency is improved; meanwhile, the environment-friendly row type pigment chromatography device has good air tightness, leakage of poisonous gas in the experiment process can be avoided, after the experiment is finished, chromatography liquid can be recycled through an injector and can be reused, waste liquid discharge is reduced, pollution to the environment is reduced, and the purposes of energy conservation and environment protection are achieved.

The utility model relates to a pigment separation experiment device which comprises a chromatography bottle, a placing frame, a medicament bottle and a first peristaltic pump, the placing frame is arranged in the chromatography bottle and divides a containing cavity into a plurality of chromatography cavities, and each chromatography cavity corresponds to a placing opening. The filter paper strip carrying the pigment filtrate fine line can be placed in the chromatography cavity through the placing opening, pigment separation experiments can be carried out in batches, mutual interference is avoided, and the chromatography effect of the pigment is guaranteed. Meanwhile, the chromatography bottle is communicated with the medicament bottle through the first conveying pipe, and the first peristaltic pump drives the first conveying pipe, so that the chromatography liquid can be added into the chromatography bottle under a closed condition, and the condition that the physical health of an operator is influenced by volatilization of toxic chromatography liquid can be avoided.

The invention discloses an experimental kit for chloroplast pigment extraction and separation and application, and belongs to the technical field of photosynthetic pigment extraction and separation, a surfactant and EDTA sodium are added during extraction, and an efficient aluminum foil silica gel thin layer and a composite chromatographic solution are adopted during separation; the chromatography medium is an efficient silica gel thin layer. In the process of extracting and separating the photosynthetic pigment, a surfactant and an EDTA (Ethylene Diamine Tetraacetic Acid) sodium metal chelating agent are added, the ratio of a chromatography medium to a chromatography liquid is optimized, a filtering and chromatography device is simplified, the extraction rate and the separation degree of the pigment are remarkably improved, the operation is simpler and safer, and finally 6-8 kinds of the photosynthetic pigment can be separated from fresh spinach leaves. 13 chloroplast pigments are separated from the leaf dry powder.

The utility model discloses a liquid storage bottle constant temperature and ventilation device for measuring lopinavir liquid chromatography, which belongs to the technical field of analysis and detection, and comprises a reaction unit, a temperature control unit and an exhaust unit, the reaction unit comprises a reaction box, a storage bottle is arranged in the reaction box, one side of the reaction box is provided with a door, and the other side of the reaction box is provided with an air inlet and an air outlet. The temperature control unit comprises a heating lamp arranged on the inner wall of the reaction box, an adjusting mechanism is arranged on the side face of the heating lamp, a power line penetrating through the reaction box is connected with the heating lamp, and the exhaust unit comprises an air guide pipe arranged at the top of the reaction box and penetrating through the interior of the reaction box. The top of the air guide pipe is provided with an air blowing mechanism, the top of the reaction box and the air guide pipe are provided with an exhaust pipe in a non-contact manner, and the device has the advantages that gas can be conveniently absorbed and subsequently treated, and the environment temperature is kept stable through the temperature control unit, so that the whole detection test is stably carried out.

The utility model relates to an amino acid paper chromatography experiment appliance, which belongs to the technical field of amino acid chromatography experiments and comprises a chromatography sample application scale, a chromatography developing vessel, a chromatography liquid small groove, a cylindrical filter paper core and circular chromatography filter paper. And the outer side is hollowed out for drawing the front edge of chromatographic liquid. According to the utility model, the design of the chromatography sample application scale helps an operator to draw a starting line of a sample application original point, a finishing line of chromatography expansion and measure the distance between the sample application original point and an amino acid color spot, so that the adverse effects of inclination and tilting of filter paper on an experiment in the traditional chromatography process are avoided from the source in the horizontal chromatography direction from the center to the circumference; the experiment efficiency and success rate are greatly improved, the chromatography vessel is smaller in size compared with a traditional chromatography device, the use amount of chromatography liquid is effectively reduced through the design of the chromatography liquid small groove, and the device is more environmentally friendly during use.

The invention discloses a method for purifying S-equol in genetic engineering escherichia coli fermentation liquor. According to the method, daidzein is used as a fermentation substrate, and S-equol-producing genetic engineering escherichia coli is used for fermenting escherichia coli BL21-G2 to obtain fermentation liquor; then, according to the characteristic of high polarity of the S-equol, extracting the S-equol generated by fermentation from the liquid phase by using ethyl acetate; and finally, separating and purifying S-equol and other soy isoflavones by using a specific ratio of chloroform and methanol chromatography liquid through a silica gel column, further separating and purifying S-equol and other impurities in the fermentation liquid through an ethyl acetate chromatography liquid, and finally obtaining S-equol with the purity close to 100%. Compared with a traditional ethanol column-passing extraction method, a one-step silica gel column-passing separation method and the like, the method is economical, practical and good in effect, the purity of the obtained S-equol is higher, and the process research prospect is wide.

The utility model discloses a chromatography liquid concentration kettle for glabridin production, which comprises a lower kettle body and an upper kettle body, the lower kettle body and the upper kettle body are fixedly connected through a connecting frame, a plurality of supporting legs are arranged on the lower kettle body, a rotating ring is arranged between the lower kettle body and the upper kettle body, a sealing ring is arranged on the rotating ring, and the lower kettle body and the upper kettle body are fixedly connected through the connecting frame. The lower kettle body and the upper kettle body are respectively provided with an annular sealing groove, each sealing ring is provided with a reinforcing mechanism playing a reinforcing role, and the lower kettle body is provided with a driving motor through a supporting plate. Due to the design that the rotating ring is matched with the driving motor, the gear and the gear ring, the direction of the feeding pipe can be freely adjusted, and production flexibility and applicability are improved.

The invention discloses a chromatographic solution for separating chloroplast pigment from leaf dry powder, a product and application, and belongs to the technical field of photosynthetic pigment separation, the chromatographic solution is composed of petroleum ether, acetone and benzene, and the volume ratio of the petroleum ether to the acetone to the benzene is 7.5: 4: 1. The chromatographic solution can separate chlorophyll a'and chlorophyll a as well as chlorophyll b 'and chlorophyll b, and compared with the chromatographic solution in the prior art, the chromatographic solution is convenient for middle school students to distinguish chlorophyll a' and chlorophyll a as well as chlorophyll b 'and chlorophyll b respectively.

The application discloses a kind of polypeptides with anti-photoaging activity, composition and application, belong to polypeptide technical field.The amino acid sequence of polypeptide with anti-photoaging activity of the present application is at least one of AVSAANDPNGI, GNTELQAA.The present application is separated and purified from phycocyanin by enzymolysis, ultrafiltration, gel chromatography, liquid chromatography separation technology, and two polypeptides with anti-photoaging activity are obtained, these polypeptides and complex peptide segment all have good tyrosinase inhibitory activity, and the tyrosinase inhibitory activity of complex peptide segment is significantly better than single peptide segment.The polypeptide obtained in the present application has good application prospect in the preparation of products with anti-photoaging effect, and lays a foundation for high value-added utilization of phycocyanin and promotes the research and application of functional active peptides.

The utility model discloses a chromatographic liquid concentration kettle for glabridin production, belongs to the field of reaction kettles, and aims to solve the problems that a kettle body and accessory equipment thereof are damaged due to the fact that the pressure is too high and cannot be discharged in time, so that part of volatile substances escape from a connector or a gap which is not tightly sealed, and material loss is caused. The pressure relief space is gradually enlarged through the bottom of the pressure relief pipe, so that pressure is relieved in good time, material loss is reduced, burdens of sealing parts, pipelines and other parts of the concentration kettle are reduced, the magnetic sleeve is magnetically connected with the magnetic ring, and the magnetic sleeve is matched with the piston block to move upwards along the outer surface of the pressure relief pipe, so that a worker can visually judge the pressure relief condition in the kettle, and the working efficiency is improved. According to the reaction kettle, the sensing element of the control switch is triggered, so that the driving fluted disc is closed, the stirring operation in the kettle is stopped, the reaction process is slowed down, and when the pressure in the kettle is reduced to a safe range, the piston block gradually moves downwards to recover to the original state.

The invention relates to a purification method of a tacrolimus crude product. The purification method comprises the following steps: obtaining an upper column liquid containing the tacrolimus crude product; an affinity resin chromatographic column is adopted for carrying out first chromatography treatment on the upper column liquid, ascomycin and dihydro tacrolimus are removed, a first chromatography liquid is obtained, the affinity resin chromatographic column contains metal ions, and the metal ions can be coordinated with tacrolimus; and carrying out secondary chromatography treatment on the first chromatography liquid by adopting macroporous adsorption resin, and removing methyl tacrolimus and a tacrolimus 8-site epimer to obtain purified tacrolimus. The purification method of the tacrolimus crude product has high purity and high yield, and is easy for industrial production.

The invention discloses a detection method and kit for rapidly detecting heavy metals in traditional Chinese medicinal materials, and belongs to the technical field of heavy metal detection in traditional Chinese medicinal materials. The detection method uses the kit for detection, and comprises the following steps: crushing medicinal materials to obtain a sample to be detected, adding the sample to be detected into a test tube 1, mixing, shaking, uniformly mixing, adding into a test tube 2, filtering, transferring to a test tube 3, dripping chromatographic liquid into a specially prepared detection card, and indicating the limit of heavy metals according to color change. The rapid detection kit for the heavy metals in the traditional Chinese medicinal materials provided by the invention is the first product capable of simultaneously detecting multiple heavy metals in the traditional Chinese medicinal materials in China, can realize multiple combined detection of four heavy metals of Cd, Pb, Hg and Cu, is simple and convenient in overall operation process, does not need to be operated by professionals, can obtain a detection result within 15 minutes, is convenient to carry, does not need to adopt precise instruments such as ICP-MS (Inductively Coupled Plasma Mass Spectrometry) and the like, and can be used for rapidly detecting the heavy metals in the traditional Chinese medicinal materials. The limit of quantitation meets the detection standard of Chinese Pharmacopoeia, and batch traditional Chinese medicinal material screening and on-site detection can be carried out.

The present invention relates to the technical field of Chinese medicine component analysis, in particular to a chromatography solution containing alcohol for Ginkgo biloba extract. 1 H NMR spectrum analysis method. The method includes the following steps: (1) preparing the test sample; (2) collecting 1 H NMR spectrum; (3) 1 H NMR spectrum signal attribution; (4) quantitative analysis. The quality control method of the present invention is reasonable, has the characteristics of simple sample preparation, rapid analysis, good reproducibility, etc., and is a major breakthrough in the quality control method of the pharmaceutical process of traditional Chinese medicine preparations.

The utility model provides a gas chromatography liquid sample injection needle anti-bending device and gas chromatography liquid sample injection needle wherein the gas chromatography liquid sample injection needle anti-bending device comprises a connecting assembly and a telescopic sleeve, one end of the connecting assembly is connected with the telescopic sleeve, the other end of the connecting assembly is connected with the sample injection needle, and the telescopic sleeve is connected with the sample injection needle. The telescopic sleeve is arranged on the periphery of the sample injection needle in a sleeving manner, the telescopic sleeve and the sample injection needle are arranged at an interval, and the telescopic sleeve can stretch out and draw back in the direction parallel to the long axis of the sample injection needle. According to the anti-bending device for the gas chromatography liquid sample injection needle, the telescopic sleeve arranged on the periphery of the sample injection needle in the sleeving manner can freely stretch out and draw back in the long axis direction in the sample injection process, and the bending risk of the needle head caused by lateral stress can be reduced. When a needle head punctures the isolation pad, the sleeve shrinks to provide axial supporting force, and external impact is dispersed; after puncture is completed, the sleeve resets to wrap the needle head, accidental collision is avoided, the probability of breakage of the sample injection needle is reduced, the service life is prolonged, and the use cost is reduced.

The utility model relates to a card shell for glycosylated hemoglobin detection card belongs to the technical field of detection card, the utility model discloses through setting upper cover, the lower cover joint forms the card shell to provide the external protection of glycosylated hemoglobin detection card, can effectively avoid sample exposure, wherein, the upper cover part is equipped with chromatography liquid tank hole and strip's sample hole, not only can guarantee the function of original drop chromatography liquid, still can simulate linear sample's process, more favorably even lateral chromatography, through setting up the reflection platform to replace the original reflection strip to realize not contact chromatography membrane, can effectively avoid the sample accumulation caused by the capillary phenomenon after adding chromatography liquid and cause the test anomaly, can improve the reflectivity, avoid external light interference.