The invention relates to a method for recovering 16-dehydropregnenolone acetate from a 16-dehydropregnenolone acetate mother liquor, wherein the method is intended to provide the method with advantages of simple technology, high recovery rate and good product quality. The method for recovering 16-dehydropregnenolone acetate from the 16-dehydropregnenolone acetate mother liquor comprises the following steps of: mother liquor condensation, solvent extraction, chromatography to remove impurities, condensation of a chromatography liquid, refining, crystallization, centrifugation and drying, to obtain 16-dehydropregnenolone acetate. The method for recovering 16-dehydropregnenolone acetate from the 16-dehydropregnenolone acetate mother liquor can make an overall yield of the 16-dehydropregnenolone acetate reach 72%, raising 4-7% compared with a traditional technology; a used reagent belongs to a common chemical product, and has advantages of easy availability, low cost, simple technology operation and convenient industrialization; and the method is used for recovering the 16-dehydropregnenolone acetate from the 16-dehydropregnenolone acetate mother liquor, wherein the purity of the 16-dehydropregnenolone acetate obtained after refining is larger than 99%, the obtained 16-dehydropregnenolone acetate can be directly used as a product, and a secondary mother liquor can be reused into the 16-dehydropregnenolone acetate production.