The invention discloses a color comparison card for color detection of new psychoactive substances and a design method thereof, and relates to the detection field of new psychoactive substances. The color comparison card of the present invention is a collection of color development result diagrams of various color reagents reacting with multiple new psychoactive substances one by one. New psychoactive substances include 15 substances including etizolam, flubromazepam, dicloazepam, and fenazepam. Chromogenic reagents include Mahal's reagent, modified Mahal's reagent, 5% ferric sulfate reagent, nitric acid-sulfuric acid reagent, gallic acid reagent, Simon's reagent, sodium nitroprusside reagent, hydroxamic acid reagent, vanillin reagent and copper sulfate reagent. Just prepare the above 10 kinds of color reagents in advance, use test tubes, beakers, glass slides, white porcelain plates or thick white cardboards to develop colors for 15 new psychoactive substances, and compare the color results with the color comparison card Yes, it is conducive to the preliminary screening of suspected new psychoactive substances on-site by criminal investigation technical inspectors.