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Detection method of new psychoactive substance BZP

A detection method and mass spectrometry technology are applied in the detection field of the new psychoactive substance BZP, which can solve the problems of complex biological sample matrix, troublesome detection work, and many interferences, and achieve the advantages of analytical operation, efficient qualitative and quantitative analysis, and high sensitivity. Effect

Inactive Publication Date: 2018-10-19
INST OF FORENSIC SCI OF MIN OF PUBLIC SECURITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the existing analysis methods focus on qualitative analysis
At the same time, in the actual investigation process, most of the detection objects required are biological samples, such as blood, urine, etc. Biological samples have the characteristics of complex matrix, many interferences, and difficult purification, which brings great trouble to the actual detection work.

Method used

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  • Detection method of new psychoactive substance BZP
  • Detection method of new psychoactive substance BZP
  • Detection method of new psychoactive substance BZP

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0074] Embodiment 1, GC-MS detects BZP in the urine sample

[0075] (1) Preparation of sample solution

[0076] An Oasis HLB solid phase extraction cartridge (60 mg, 3 mL) was first pretreated with 2 mL of methanol, 2 mL of deionized water and 2 mL of ammonium acetate (10 mM, pH=9.5). After sample loading, the solid-phase extraction cartridge was washed with 1 mL of deionized water, and then dried in vacuum. Elute with 1 mL of methanol, blow to dryness with nitrogen, redissolve with methanol to 400 μL, filter and centrifuge, and put into a sample vial.

[0077] (2) Setting of detection conditions

[0078] The setting process of gas chromatography conditions is as follows:

[0079] The carrier gas is helium;

[0080] Chromatographic column is DB-5MS or HP-5MS;

[0081] The heating program is as follows: the initial temperature is 70°C, rising to 300°C at a rate of 10°C / min, and keeping for 7 minutes;

[0082] Mass spectrometry conditions: the voltage scanning range is set...

Embodiment 2

[0086] Embodiment 2, gas chromatography detects the BZP in the hair

[0087] (1) Preparation of sample solution

[0088] Basically the same as in Example 1.

[0089] (2) Preparation of standard working solution

[0090] Take the BZP standard stock solution and dilute it sequentially into a series of standard solutions with mass concentrations of 1.0, 0.5, 0.25, 0.125, 0.1, 0.05, 0.01, 0.005, and 0.001 mg / mL.

[0091] Take 1 μL of standard solutions of different concentrations and inject them, and record the corresponding peak area.

[0092] Each concentration was injected 3 times, and linear regression was performed on the average A of the 3 peak areas and the mass concentration c (mg / mL) to obtain the regression equation of BZP: A=622.26c+0.0716, r 2 It is 0.9966, the linear range is 5~1000μg / mL, and the detection limit is 5μg / mL (S / N≥3).

[0093] (3) Setting of detection conditions

[0094] The carrier gas is nitrogen;

[0095] Chromatographic column is DB-5 or HP-5; ...

Embodiment 3

[0107] Embodiment 3, liquid chromatography detects BZP in blood

[0108] (1) Preparation of sample solution

[0109] Basically the same as in Example 1.

[0110] (2) Preparation of standard working solution

[0111] Take the BZP standard stock solution and dilute it sequentially into a series of standard solutions with mass concentrations of 1.0, 0.75, 0.5, 0.1, 0.05, 0.025, 0.01, 0.005, 0.001, and 0.0005 mg / mL.

[0112] Take 10 μL of standard solutions of different concentrations and inject them, and record the corresponding peak area.

[0113] Each concentration was injected 3 times, and linear regression was performed on the average A of the peak area of ​​the 3 times and the mass concentration c (mg / mL), and the regression equation of BZP was A=89079c+117849, r 2 =0.999 6, the linear range is 5-1000 μg / mL, the limit of quantification is 5 μg / mL (S / N≥3).

[0114] (3) Setting of detection conditions

[0115] The column is 300SB-C18 (4.6×150mm, 5μm);

[0116] The colu...

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Abstract

The invention discloses a detection method of a new psychoactive substance BZP. The method for accurate and efficient determination of BZP in a biological sample includes gas chromatography-mass spectrometry, gas chromatography, liquid chromatography, liquid chromatography-mass spectrometry and other qualitative and quantitative analysis techniques. Due to a lot of interference factors in the biological sample, the pretreatment process of the inspected material is very important. Through a large number of experiments, and by a solid-phase extraction process, the BZP in the biological sample can be fully extracted, and high-efficiency qualitative and quantitative analysis can be achieved. The analytical methods have high sensitivity and wide linear range, and the detector is low in cost, thus being conducive to automatic analysis operation.

Description

technical field [0001] The invention relates to a detection method of a new psychoactive substance BZP, which belongs to the field of criminal investigation drug detection. Background technique [0002] BZP (benzylpiperazine) belongs to the piperazine class of new psychoactive substances with the molecular formula C 11 h 16 N 2 , the molecular weight is 176.258g / mol, and its structural formula is shown in formula (1). BZP is a recreational drug that produces euphoria and stimulation. BZP produces effects similar to amphetamines. Reported adverse reactions include acute psychosis, nephrotoxicity, and seizures. No deaths were reported from BZP intake alone, but at least 2 deaths were caused by the combined use of BZP and MDMA. Its sale is banned in many countries, including Australia, New Zealand, the United States, Ireland, the United Kingdom, Bulgaria, Romania and other countries in Europe. my country listed BZP as a class of psychotropic drugs in the 2013 edition of ...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/045G01N2030/062
Inventor 常颖赵阳高利生郑珲张春水翟晚枫李彭赵彦彪郑晓雨杨虹贤刘克林钱振华
Owner INST OF FORENSIC SCI OF MIN OF PUBLIC SECURITY
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