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Detection method of novel psychoactive substance mCPP

A detection method, mass spectrometry technology, applied in the field of detection of new psychoactive substances mCPP, can solve the problems of complex biological sample matrix, troublesome detection work, difficult purification, etc., and achieve favorable analysis operation, high-efficiency qualitative and quantitative analysis, and low cost Effect

Inactive Publication Date: 2018-09-11
INST OF FORENSIC SCI OF MIN OF PUBLIC SECURITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the existing analysis methods focus on qualitative analysis
At the same time, in the actual investigation process, most of the detection objects required are biological samples, such as blood, urine, etc. Biological samples have the characteristics of complex matrix, many interferences, and difficult purification, which brings great trouble to the actual detection work.

Method used

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  • Detection method of novel psychoactive substance mCPP
  • Detection method of novel psychoactive substance mCPP
  • Detection method of novel psychoactive substance mCPP

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0074] Embodiment 1, detection of mCPP in urine sample by gas chromatography-mass spectrometry

[0075] (1) Preparation of sample solution

[0076] An Oasis HLB solid phase extraction cartridge (60 mg, 3 mL) was first pretreated with 2 mL of methanol, 2 mL of deionized water and 2 mL of ammonium acetate (10 mM, pH=9.5). After sample loading, the solid-phase extraction cartridge was washed with 1 mL of deionized water, and then dried in vacuum. Elute with 1 mL of methanol, blow to dryness with nitrogen, redissolve with methanol to 400 μL, filter and centrifuge, and put into a sample vial.

[0077] (2) Setting of detection conditions

[0078] The setting process of gas chromatography conditions is as follows:

[0079] The carrier gas is helium;

[0080] Chromatographic column is DB-5MS or HP-5MS;

[0081] The heating program is as follows: the initial temperature is 70°C, rising to 300°C at a rate of 10°C / min, and keeping for 7 minutes;

[0082] Mass spectrometry condition...

Embodiment 2

[0086] Embodiment 2, gas chromatographic detection of mCPP in hair

[0087] (1) Preparation of sample solution

[0088] Basically the same as in Example 1.

[0089] (2) Preparation of standard working solution

[0090] Take the mCPP standard stock solution and dilute it sequentially into a series of standard solutions with mass concentrations of 1.0, 0.5, 0.25, 0.125, 0.1, 0.05, 0.01, 0.005, and 0.001 mg / mL.

[0091] Take 1 μL of standard solutions of different concentrations and inject them, and record the corresponding peak area.

[0092] Each concentration was injected 3 times, and linear regression was performed on the average A of the peak areas of the 3 times and the mass concentration c (mg / mL) to obtain the regression equation of mCPP: A=548.65c-5.1071, r 2 It is 0.9997, the linear range is 5~1000μg / mL, and the detection limit is 5μg / mL (S / N≥3).

[0093] (3) Setting of detection conditions

[0094] The carrier gas is nitrogen;

[0095] Chromatographic column is D...

Embodiment 3

[0107] Embodiment 3, liquid chromatographic detection of mCPP in blood

[0108] (1) Preparation of sample solution

[0109] Basically the same as in Example 1.

[0110] (2) Preparation of standard working solution

[0111] Take the mCPP standard stock solution and dilute it sequentially into a series of standard solutions with mass concentrations of 1.0, 0.75, 0.5, 0.1, 0.05, 0.025, 0.01, 0.005, 0.001, and 0.0005 mg / mL.

[0112] Take 10 μL of standard solutions of different concentrations and inject them, and record the corresponding peak area.

[0113] Each concentration was injected 3 times, and linear regression was performed on the average A of the 3 peak areas and the mass concentration c (mg / mL), and the regression equation of mCPP was A=485.03c-5666.1, r 2 =0.9997, the linear range is 5-1000 μg / mL, the limit of quantification is 5 μg / mL (S / N≥3).

[0114] (3) Setting of detection conditions

[0115] The column is 300SB-C18 (4.6×150mm, 5μm);

[0116] The column te...

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Abstract

The invention discloses a detection method of a novel psychoactive substance mCPP. The method for precisely and efficiently testing the mCPP in a biological sample comprises qualitative and quantitative analysis methods of gas chromatography mass spectrometry, gas phase chromatography, liquid phase chromatography, liquid chromatography-mass spectrometry and the like. The related biological samplescan be at least one kind of materials in blood, urine, saliva and hair. Since the interface factors of biological samples are many, the pretreatment process of inspection materials is very important.Through a great number of experiments, the mCPP in the biological samples can be sufficiently extracted through the solid phase extraction process; the efficient qualitative and quantitative analysisis realized. Each analysis method has high sensitivity and wide linear range; the detector cost is low; the automatic analysis operation is facilitated.

Description

technical field [0001] The invention relates to a detection method of a new psychoactive substance mCPP, which belongs to the field of criminal investigation drug detection. Background technique [0002] mCPP (1-3-chlorophenylpiperazine) belongs to the piperazine class of new psychoactive substances, which can produce excitatory and hallucinogenic effects similar to MDMA. First discovered for scientific research in the 1970s, it was sold as a curated drug in the mid-2000s. It was detected in pills in New Zealand as a legal alternative to illegal stimulants. Sold under the name "ecstasy" in Europe and the United States. Its structural formula is shown in formula (1). [0003] [0004] By consulting a large number of domestic and foreign literatures, the reported analysis methods of mCPP include color reaction, microcrystal experiment, thin layer chromatography, gas chromatography, high performance liquid chromatography, capillary electrophoresis and Fourier transform in...

Claims

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Application Information

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IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 常颖赵阳高利生郑珲张春水翟晚枫李彭赵彦彪郑晓雨杨虹贤刘克林钱振华
Owner INST OF FORENSIC SCI OF MIN OF PUBLIC SECURITY
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