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Detection method of new psychoactive substance MDBZP

A detection method, mass spectrometry technology, applied in the field of criminal investigation drug detection, can solve the problems of complex matrix of biological samples, troublesome detection work, difficult purification, etc., and achieve the effect of favorable analysis operation, high-efficiency qualitative and quantitative analysis, and low cost

Pending Publication Date: 2018-09-14
INST OF FORENSIC SCI OF MIN OF PUBLIC SECURITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the existing analysis methods focus on qualitative analysis
At the same time, in the actual investigation process, most of the detection objects required are biological samples, such as blood, urine, etc. Biological samples have the characteristics of complex matrix, many interferences, and difficult purification, which brings great trouble to the actual detection work.

Method used

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  • Detection method of new psychoactive substance MDBZP
  • Detection method of new psychoactive substance MDBZP
  • Detection method of new psychoactive substance MDBZP

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0074] Embodiment 1, GC-MS detects MDBZP in the urine sample

[0075] (1) Preparation of sample solution

[0076] An Oasis HLB solid phase extraction cartridge (60 mg, 3 mL) was first pretreated with 2 mL of methanol, 2 mL of deionized water and 2 mL of ammonium acetate (10 mM, pH=9.5). After sample loading, the solid-phase extraction cartridge was washed with 1 mL of deionized water, and then dried in vacuum. Elute with 1 mL of methanol, blow to dryness with nitrogen, redissolve with methanol to 400 μL, filter and centrifuge, and put into a sample vial.

[0077] (2) Setting of detection conditions

[0078] The setting process of gas chromatography conditions is as follows:

[0079] The carrier gas is helium;

[0080] Chromatographic column is DB-5MS or HP-5MS;

[0081] The heating program is as follows: the initial temperature is 70°C, rising to 300°C at a rate of 10°C / min, and keeping for 7 minutes;

[0082] Mass spectrometry conditions: the voltage scanning range is s...

Embodiment 2

[0086] Embodiment 2, gas chromatography detects the MDBZP in the hair

[0087] (1) Preparation of sample solution

[0088] Basically the same as in Example 1.

[0089] (2) Preparation of standard working solution

[0090] Take the MDBZP standard stock solution and dilute it successively into a series of standard solutions with mass concentrations of 1.0, 0.5, 0.25, 0.125, 0.1, 0.05, 0.01, 0.005, and 0.001 mg / mL.

[0091] Take 1 μL of standard solutions of different concentrations and inject them, and record the corresponding peak area.

[0092] Each concentration was injected 3 times, and linear regression was performed on the average A of the 3 peak areas and the mass concentration c (mg / mL) to obtain the regression equation of MDBZP: A=505.99c-49042, r 2 It is 0.9999, the linear range is 5~1000μg / mL, and the detection limit is 5μg / mL (S / N≥3).

[0093] (3) Setting of detection conditions

[0094] The carrier gas is nitrogen;

[0095] Chromatographic column is DB-5 or HP...

Embodiment 3

[0107] Embodiment 3, liquid chromatography detects MDBZP in the blood

[0108] (1) Preparation of sample solution

[0109] Basically the same as in Example 1.

[0110] (2) Preparation of standard working solution

[0111] Take the MDBZP standard stock solution and dilute it sequentially into a series of standard solutions with mass concentrations of 1.0, 0.75, 0.5, 0.1, 0.05, 0.025, 0.01, 0.005, 0.001, and 0.0005 mg / mL.

[0112] Take 10 μL of standard solutions of different concentrations and inject them, and record the corresponding peak area.

[0113] Each concentration is sampled 3 times, and linear regression is performed on the average value A of the peak area of ​​the 3 times and the mass concentration c (mg / mL), and the regression equation of MDBZP is A=7250.7c-75238, r 2 =0.9992, the linear range is 5-1000 μg / mL, the limit of quantification is 5 μg / mL (S / N≥3).

[0114] (3) Setting of detection conditions

[0115] The column is 300SB-C18 (4.6×150mm, 5μm);

[011...

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Abstract

The invention discloses a detection method of a new psychoactive substance MDBZP. The method, provided by the invention, for accurately and efficiently determining the MDBZP in biological samples comprises qualitative and quantitative analysis methods including gas chromatography-mass spectrometry, gas chromatography, liquid chromatography, liquid chromatography-mass spectrometry, and the like; the biological samples can be at least one of blood, urine, saliva and hair. Due to the large number of interfering factors in the biological samples, the pretreatment process of the tested materials isvery important. By means of a great number of experiments, the detection method effectively and fully extracts MDBZP in the biological samples by using a solid phase extraction process; the high-efficiency qualitative and quantitative analysis is realized; the analysis methods are high in sensitivity and wide in linear range. A detector is low in cost and beneficial to automatic analysis operation.

Description

technical field [0001] The invention relates to a detection method of a new psychoactive substance MDBZP, which belongs to the field of criminal investigation drug detection. Background technique [0002] MDBZP (1-(3,4-methylenedioxybenzyl)piperazine) belongs to the piperazine class of new psychoactive substances with the molecular formula C 12 h 18 N 2 o 2 , the molecular weight is 190.29g / mol, and its structural formula is shown in formula (1). It is marketed as a planner drug and is an ingredient in street ecstasy. However, MDBZP is weakly stimulating and does not produce euphoric or hallucinogenic effects. The side effects it produced were dizziness, nausea and headaches, and high doses caused convulsions. [0003] [0004] By consulting a large number of domestic and foreign literatures, it has been reported that the analysis methods of MDBZP include color reaction, microcrystal experiment, thin layer chromatography, gas chromatography, high performance liquid ...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/72G01N30/06
CPCG01N30/02G01N30/06G01N30/72G01N2030/062
Inventor 常颖赵阳高利生郑珲张春水翟晚枫李彭赵彦彪郑晓雨杨虹贤刘克林钱振华
Owner INST OF FORENSIC SCI OF MIN OF PUBLIC SECURITY
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