Surface-enhanced Raman scattering (SERS) method for rapidly detecting new psychoactive substances in human urine

A detection method and technology for active substances, applied in Raman scattering, material excitation analysis, etc., can solve the problem of lack of rapid detection methods, and achieve the effect of fast speed, good stability and reduced dilution

Inactive Publication Date: 2017-08-18
安徽中科赛飞尔科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Due to the simple preparation of new psychoactive substances, the fast renovation speed, and the lack of corresponding rapid detection methods

Method used

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  • Surface-enhanced Raman scattering (SERS) method for rapidly detecting new psychoactive substances in human urine
  • Surface-enhanced Raman scattering (SERS) method for rapidly detecting new psychoactive substances in human urine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] (1) Synthesize the gold nanorod sol with the maximum absorption peak of UV-visible absorption near 785 nm and SERS activity;

[0043] (2) Drop the cleaned gold nanorods on a clean hydrophobic silicon wafer and dry to obtain a SERS substrate;

[0044] (3) Take 1 mL of urine containing narcotics in a 4 mL centrifuge tube, add 100 μL of nitric acid solution to it, shake well and let stand for 5 minutes, then add 100 μL of sodium bicarbonate solution and 100 μL of cyclohexane extractant, and shake to extract;

[0045] (4) After standing still for 1.5 min, take the organic layer solution;

[0046] (5) Drop the organic layer solution onto the SERS substrate in step (2), and use a portable Raman spectrometer for SERS detection.

[0047] The ultraviolet-visible absorption spectrum (Uv-vis) and field emission transmission scanning electron microscope (TEM) pictures of the gold nanorod sol synthesized in this example are as follows figure 1 As shown in A and B, from figure 1 I...

Embodiment 2

[0050] (1) Synthesize the gold nanorod sol with SERS activity with the maximum absorption peak of UV-Vis at around 785 nm;

[0051] (2) Drop the cleaned gold nanorods on a clean hydrophobic silicon wafer and dry to obtain a SERS substrate;

[0052] (3) Take 1 mL of urine containing narcotics in a 4 mL centrifuge tube, add 100 μL of nitric acid solution to it, shake well and let stand for 4 minutes, then add 10 μL of sodium acetate, then add 50 μL of chloroform extractant, and shake to extract;

[0053] (4) After standing for 1 min, take the organic layer solution;

[0054] (5) Drop the organic layer solution onto the SERS substrate in step (2), and use a portable Raman spectrometer for SERS detection.

Embodiment 3

[0056] (1) Synthesize the gold nanorod sol with SERS activity with the maximum absorption peak of UV-Vis at around 785 nm;

[0057] (2) Drop the cleaned gold nanorods on a clean hydrophobic silicon wafer and dry to obtain a SERS substrate;

[0058] (3) Take 1 mL of urine containing narcotics in a 4 mL centrifuge tube, add 300 μL of nitric acid solution to it, shake well and let stand for 6 minutes, then add 200 μL of ammonia water and 200 μL of ethyl acetate, and shake to extract;

[0059] (4) After standing for 2 minutes, take the organic layer solution;

[0060] (5) Drop the organic layer solution onto the SERS substrate in step (2), and use a portable Raman spectrometer for SERS detection.

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Abstract

The invention provides a surface-enhanced Raman scattering (SERS) method for rapidly detecting new psychoactive substances in human urine, which comprises the following steps: synthesizing gold nanorod colloidal sols with SERS activity; dripping the cleaned gold nanorod colloidal sols onto a clean hydrophobic silicon wafer, and drying to obtain an SERS substrate; collecting human urine, adding a nitric acid solution, shaking, and standing for 4-6 min; adding a slightly alkaline solution and an organic extractant, and carrying out shaking extraction; standing for 1-2 min, and collecting an organic layer solution; and dripping the organic layer solution onto the SERS substrate, and performing SERS detection with a portable Raman spectrometer. Through pretreatment of toxins in human urine, the method disclosed by the invention can rapidly purify and enrich new psychoactive substances in human urine, and the detection is sensitive and rapid and can be completed within 10 min.

Description

technical field [0001] The invention relates to a drug detection method, in particular to a method for detecting new psychoactive substances in human urine by using surface-enhanced Raman spectroscopy (SERS). Background technique [0002] New psychoactive substances, also known as "designer drugs" or laboratory drugs, are drug analogues obtained by modifying the chemical structure of scheduled drugs in order to avoid crackdowns. "legal" drugs such as hallucinations, narcotics, and even stronger effects. The World Office on Drugs and Crime predicts that new psychoactive substances may become the third class of drugs in the world. According to the 2016 World Drug Situation Report: During 2008-2015, about 644 new psychoactive substances were reported. As of October 2015, China has listed 166 new psychoactive substances. From a legal perspective, psychotropic substances have not yet been included in the scope of legal control and are a potential drug. Generally, new psycho...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65
Inventor 杨良保孟娟于博荣毛妹陈诚
Owner 安徽中科赛飞尔科技有限公司
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