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2114results about How to "Easy to sample" patented technology

Serum/plasma micronas and uses thereof

This invention provides a combination of microRNAs for evaluating the physiological and/or pathological condition of a subject, wherein the combination comprises all detectable microRNAs stably existing in the serum/plasma of a subject; and a method for evaluating the physiological and/or pathological condition of a subject, wherein the method includes determining all detectable microRNAs stably existing in the serum/plasma of a subject; and a kit for evaluating the physiological and/or pathological condition of a subject, wherein the kit contains the tools for determining all detectable microRNAs that stably existing in the serum/plasma of a subject; and a biochip for evaluating the physiological and/or pathological condition of a subject, wherein the biochip contains the components for determining all detectable microRNAs stably existing in the serum/plasma of a subject. The aforementioned combination, method, kit and biochip can be used for diagnosis as well as differentially diagnosis of diseases including various tumors; various acute/chronic infectious diseases, e.g. viral diseases such as viral influenza, viral hepatitis, AIDS, SARS, bacterial diseases such as tuberculosis, bacterial pneumonia, and other acute/chronic infectious diseases caused by various pathogenic microorganisms; other acute/chronic diseases such as diseases of respiratory system, diseases of immune system, diseases of blood and hematopoietic system, diseases of circulatory system such as cardio-cerebrovascular diseases, metabolic diseases of endocrine system, diseases of digestive system, diseases of nervous system, diseases of urinary system, diseases of reproductive system and diseases of locomotor system, prediction of complications occurrence and malignant diseases relapse, evaluation of therapeutic effects, screening of pharmaceutical active ingredients, assessment of drug efficacy as well as forensic authentication and prohibited drug inspection and the like, possessing a number of advantages such as extensive detection spectrum, high sensitivity, low cost, convenience for sampling, ease for sample preservation, etc. The said method can be widely used in work related to general survey of diseases and so on, improve the low-specificity and low-sensitivity caused by individual differences which single markers are difficult to overcome, significantly increasing the clinical detection rate of diseases, all of which make it become an effective means for diagnosing diseases in an early phase.
Owner:JIANGSU MINGMA BIOTECH

Primer pair, probe and kit used for noninvasive polygene methylation combination detection for early stage colorectal cancer and applications thereof

The invention relates to a primer pair and a probe used for noninvasive polygene methylation combination detection for early stage colorectal cancer, and includes the primer pair and the probe used for detecting methylation of genes Spetin9, NDRG4, BMP3, THBD and SDC2 and the primer pair and the probe for internal reference ACTB; the sequences of the primer pair and the probe are represented as the SEQ ID No.1 to the SEQ ID No.18. The invention also provides a kit containing the primer pair and the probe and applications thereof. The application method includes free DNA extraction from a plasma specimen, sulfite conversion, PCR amplification reaction, fluorescent signal detection and result determination. The kit and the method are suitable for methylation detection of the five genes Spetin9, THBD, SDC2, NDRG4 and BMP3 in human peripheral blood; compared with a conventional colorectal cancer diagnosis method, the application method fully utilizes the free DNA extraction from a plasma specimen, the DNA methylation and QPCR associated technologies, thus developing the kit having high sensitivity and specificity. The primer pair, probe and kit are used for performing early stage noninvasive screening to human colorectal cancer.
Owner:上海酷乐生物科技有限公司

Portable shallow soft soil original state sampling device and sampling method

The invention discloses a portable shallow soft soil original state sampling device and a sampling method. A solution is provided for solving the problems that soft soil with a high water content is likely to fall along the inner wall of a sampler, an original state soil column is easily disturbed in repairing, separating and post-treating processes, and the inner cavity of the sampler is difficult to clean and the like. The sampling device comprises the sampler, a positioner and a separator. Smooth sampling is realized through a sampling rod, a steering base, a force transfer base and a sampling barrel; a negative pressure is generated in the inner cavity of the force transfer base by virtue of a piston and a baffle plate, so that a soil sample is prevented from falling. By opening out a liner pipe and the separator, the soil column is repaired, separated and packaged. The method comprises the following steps: removing miscellaneous soil in the surface layer, assembling the sampling device, and pressing the sampler into a specific depth; then lifting up the sampling rod to close the inner cavity of the force transfer seat; and screwing the sampler out, detaching the sampling barrel, pushing the liner pipe out by using the separator and performing packaging with a cover. The device disclosed by the invention is simple in structure, convenient to operate, small in sampling disturbance and high in efficiency.
Owner:JILIN UNIV

Water quality automatic sampling system based on rotor unmanned plane and method thereof

The invention discloses a water quality automatic sampling system based on a rotor unmanned plane and a method thereof, and applicable to the technical field of water quality sampling and detection. The water quality automatic sampling system mainly comprises a turntable motor (1), a depth detecting sensor (9), a sampling pump (12), a sampling bottle (16), a solenoid valve (17), an unmanned plane (19), a rocker arm (20), and a controller (21); the turntable motor drives the sampling pipe and adjusts the sampling depth; the sampling pipe is connected with a plurality of sampling bottles through a branch, and the sampling bottles are arranged in the middle of the sampling platform base; the platform base is connected with the unmanned plane through the rocker arm; through bending the rocker arm, the flying model and the traction model of the unmanned vehicle can be switched; compensation bending is performed on the rocker arm monitored and controlled on the basis of a gradiograph. The system and the method can realize the organic combination of water quality sampling and the unmanned vehicle, fully use the loading and cruising ability of the unmanned vehicle, effectively keep the balance property of the sampling system, and are featured by fast sampling, convenience, and high efficiency.
Owner:NANCHANG UNIV

Experimental device for washing heavy metal pollution and method thereof

The invention relates to a leaching experimental device and the method of heavy metal pollution soil, the experimental device comprises: a leaching solution storage, a peristaltic pump, a leaching column group, a hose, a three-way glass tube, a hose clip and a filter liquor collector. The end of the hose arranged on the peristaltic pump input is immersed under the storage liquid level, the hose arranged on the output of the peristaltic pump is connected with the leaching column group; the leaching column group comprises a plurality of leaching columns, the leaching columns are connected with head and tail of each other through the hose and the three-way glass tube, the projecting end of the three glass tube is provided with a hose clip; the column of the leaching column is made of perspex tube, the upper and lower port of the perspex tube is respectively provided with a nylon membrane, an O-type pad and a button, a connecting hose is extracted in the centre of the button, the connecting hose is connected with the three-way glass tube through the hose; the lower end of the leaching column group is connected with the filter liquor collector through the hose. The leaching experimental device and the method has the advantages of determining and sampling the solution concentration of different soil column depth, changing and leaching effect, and convenient measurement.
Owner:SHENYANG UNIV
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