Stem cell sampling device and sampling method thereof

Abstract

The invention belongs to the technical field of stem cell sampling, and particularly relates to a stem cell sampling device and a sampling method thereof.The stem cell sampling device comprises a machine shell and further comprises a test tube rack, a sterilizing lamp, an adjusting device, a sampling device and a cleaning device which are installed in the machine shell. According to the stem cell sampling device and the sampling method thereof, by arranging the sampling device, after a suction push handle on one sampling side ascends, generated suction force can be transmitted to a sampling suction pipe, automatic sampling is achieved, a driving frame can be driven to ascend and descend through rotation of a driving lead screw, and a storage hose is driven to deflect; the self-locking block can be driven by rotation of the locking lead screw to move relatively, the sucked sample can be prevented from entering a conveying pipeline after one end of the storage hose is sealed, and meanwhile, when suction is needed, the self-locking block is opened, so that the self-locking block is opened, and the self-locking block can be conveniently locked. And gas circulation can be facilitated.

Description

Technical Field

[0001] The present invention relates to the technical field of stem cell sampling, and in particular to a stem cell sampling device and a sampling method thereof. Background Art

[0002] Stem cells are a type of multipotent cells with the ability to self-replicate. Under certain conditions, stem cells can differentiate into a variety of functional cells. They are a type of primitive undifferentiated cells with multidirectional differentiation potential and self-replication ability.

[0003] Existing research on stem cells in the laboratory requires quantitative sampling of a large number of blood samples and separation by centrifuge, and further purification of stem cells using flow cytometry or magnetic sorting technology. Generally, sampling is done manually using sampling tools and then placed in a separation machine for centrifugation, which reduces work efficiency. The sampling tools need to be disinfected and cleaned before the next sampling can be carried out. The operation is cumbersome, and manual mechanical operation is easy to fatigue. Finally, manual sampling is prone to sample contamination, affecting the final experimental results. Summary of the invention

[0004] Based on the existing manual sampling, work efficiency is reduced, and the sampling tools need to be disinfected and cleaned, which is cumbersome. At the same time, manual mechanical operation is easy to cause fatigue. Finally, manual sampling is easy to cause sample contamination, affecting the final experimental results. The present invention proposes a stem cell sampling device and a sampling method thereof.

[0005] The present invention provides a stem cell sampling device, which comprises a housing, and also comprises a test tube rack, a disinfection lamp, an adjustment device, a sampling device and a cleaning device installed in the housing.

[0006] The adjusting device is located inside the housing and adjusts the position of the sampling device. The adjusting device includes an adjusting hydraulic cylinder. The lifting and lowering of the adjusting hydraulic cylinder drives the sampling device to be lifted and lowered.

[0007] The sampling device is located on the outer surface of the regulating device, and automatically samples the test tube containing the sample. The sampling device includes a sampling mechanism, a driving mechanism and a suction mechanism. The sampling mechanism includes a sampling pipette and a storage hose with a collar. The sampling pipette sucks the sample in the test tube containing the sample into the storage hose. The driving mechanism includes a driving frame. The lifting and lowering of the driving frame drives the storage hose to deflect. The suction mechanism includes a suction push handle. The lifting and lowering of the suction push handle drives the sampling pipette to suction.

[0008] The cleaning device is located inside the housing and cleans the sampling pipette and the storage hose. The cleaning device comprises a cleaning nozzle, and the cleaning nozzle flushes the outer surface of the sampling pipette.

[0009] Preferably, the adjusting device comprises a supporting sleeve, the supporting sleeve is fixedly mounted on the inner wall of the casing, the inner wall of the supporting sleeve is fixedly mounted on one end of the piston rod of the adjusting hydraulic cylinder, and a lifting plate is fixedly mounted on one end of the piston rod of the adjusting hydraulic cylinder.

[0010] Through the above technical scheme, the suction pipette can be driven to descend by the adjusting hydraulic cylinder in the supporting sleeve, and can be inserted into the test tube with the sample to facilitate sampling. The suction pipette that has completed sampling can be driven into the separation equipment to facilitate the transportation of the sampled sample. At the same time, the suction pipette that has completed transportation can be lowered for cleaning, thereby improving work efficiency.

[0011] Preferably, the upper surface of the supporting sleeve is rotatably connected to a limiting sleeve with a gear ring through a bearing, an adjusting motor is fixedly installed on the inner wall of the casing, one end of the output shaft of the adjusting motor drives one end of the limiting sleeve to rotate through a gear, and a fixing plate distributed in a ring array is slidably inserted into the inner wall of the limiting sleeve, the upper surface of the fixing plate contacts the lower surface of the lifting plate, a reset spring is fixedly installed on the lower surface of the fixing plate, and one end of the reset spring is fixedly installed on the inner wall of the limiting sleeve.

[0012] Through the above technical solution, by adjusting the drive of the motor, the limiting sleeve can be driven to rotate, thereby driving the fixed plate to rotate and then realize switching work. While sampling, another sampling straw can transport the sample, thereby improving work efficiency.

[0013] Preferably, the sampling straw is threadedly connected to one end of the fixed plate, one end of the sampling straw is fixedly connected to one end of the storage hose, one end of the storage hose is fixedly connected to a delivery pipe with an adsorption ring, and the delivery pipe is fixedly installed on the upper surface of the limiting sleeve through a bracket.

[0014] Through the above technical solution, the sampled samples can be stored in the storage hose, and the storage hose can be easily deflected to prevent the sampled samples from flowing back, and can be quickly transported out after the sampled samples need to be transported.

[0015] Preferably, the driving mechanism further comprises a driving screw with a gear, the driving screw is rotatably connected to the outer surface of the fixing plate through a bearing, the driving frame is threadedly connected to one end of the driving screw, and the two ends of the driving frame are hinged to the outer surface of the sleeve ring of the storage hose through a pin shaft, the outer surface of the driving frame and the outer surface of the fixing plate are slidably inserted into the outer surface of the driving frame through a limit block, and the outer surface of the driving frame is rotatably connected to a locking screw through a bearing, the outer surface of the locking screw is threadedly connected to a self-locking block, one end of the self-locking block is slidably inserted into one end of the limit rod of the driving frame, and the outer surface of the driving frame is rotatably connected to a transmission rod with a gear through a bearing, one end of the transmission rod drives the locking screw to rotate through a bevel gear set, and the outer side surface of the supporting sleeve is fixedly installed with a lifting hydraulic cylinder through a connecting frame, and the piston rod of the lifting hydraulic cylinder is fixedly installed with a driving motor through a connecting plate, and one end of the output shaft of the driving motor drives the transmission rod and the driving screw to rotate through a gear.

[0016] Through the above technical scheme, the rotation of the driving screw can drive the driving frame to rise and fall, and drive the storage hose to deflect, so that the sucked sample will not flow back. At the same time, the sample can be quickly transported when it is needed. The rotation of the locking screw can drive the self-locking block to move relative to each other, so that after one end of the storage hose is sealed, the sucked sample can be prevented from entering the delivery pipeline. At the same time, when suction is needed, the self-locking block can be opened quickly to facilitate the circulation of gas. The lifting and lowering can be achieved by the driving motor, and the transmission rod or the driving screw can be driven to rotate by a driving motor.

[0017] Preferably, the suction mechanism also includes a split sleeve, which is fixedly installed on the inner bottom wall of the casing, and a plug-in rod is fixedly installed on the inner top wall of the casing, and a threaded sleeve is slidably sleeved on the outer surface of the plug-in rod, and the upper surface of the split sleeve is rotatably connected to a rotating gear through a bearing, and adjacent rotating gears are meshed, one end of the threaded sleeve is threadedly connected to the inner wall of the rotating gear, and the outer surface of the suction push handle is rotatably connected to one end of the threaded sleeve through a bearing.

[0018] Through the above technical scheme, the dividing sleeve is divided into four grooves, and the inner grooves can have different functions. The rotation of the rotating gear can drive the threaded sleeve to rise and fall, thereby pushing the suction push handle to rise and fall, and the adjacent rotating gears can rotate synchronously in the opposite direction, thereby driving the suction push handle to rise and fall to achieve the action of suction or pushing, and at the same time, the disinfectant can be sucked and pushed, thereby achieving sampling, sample transportation and cleaning of the storage hose and the suction straw.

[0019] Preferably, a rotating motor is fixedly mounted on the outer surface of the split sleeve, a worm is fixedly mounted on one end of the output shaft of the rotating motor, a worm wheel is fixedly mounted on the outer surface of one of the rotating gears, and the worm is meshed with the worm wheel.

[0020] Through the above technical solution, the rotating motor can drive the worm to rotate, thereby driving the worm wheel to rotate, and then driving a rotating gear to rotate. The rotating motor is a variable speed motor, and the speed of suction and pushing can be changed after adjusting the speed of the rotating motor, so that the suction push handle can be quickly reset when the fixed plate is switched.

[0021] Preferably, one end of the split sleeve is slidably plugged with a plug-in connecting tube, the outer surface of the plug-in connecting tube is fixedly installed with an adsorption electromagnet, the outer surface of the adsorption electromagnet is magnetically connected to the adsorption ring of the conveying pipe, and the outer surface of the plug-in connecting tube is fixedly installed with a connecting spring, one end of the connecting spring is fixedly installed with one end of the split sleeve.

[0022] Through the above technical scheme, after the adsorption electromagnet is magnetically connected to the adsorption ring of the conveying pipeline, it is convenient to plug the connecting pipe into the conveying pipeline, so that the suction force generated by the rising of the suction push handle in the split sleeve enters the conveying pipeline and is transmitted to the sampling pipette.

[0023] Preferably, the cleaning device also includes a storage tank, which is fixedly mounted on the upper surface of the casing, and the lower surface of the storage tank is fixedly connected to the inner wall of the split sleeve through a connecting pipe with a control valve, a one-way valve is fixedly mounted on the inner wall of the split sleeve, a cleaning trough body with a water outlet pipe is fixedly mounted on the inner wall of the casing, the inner wall of the cleaning trough body is fixedly mounted to the outer surface of the cleaning nozzle, a water pump is fixedly mounted on the inner wall of the casing, the water inlet end of the water pump is fixedly connected to the outer surface of the storage tank, the water outlet end of the water pump is fixedly connected to the outer surface of the cleaning nozzle, a collecting trough body with a water outlet pipe is fixedly mounted on the inner bottom wall of the casing, and the water outlet pipe of the collecting trough body is fixedly connected to the water outlet pipe of the cleaning trough body.

[0024] Through the above technical scheme, disinfectant can be transported into the split sleeve through the storage tank, and disinfectant can be transported into the cleaning nozzle at the same time, so that the outer surface of the sampling straw can be rinsed and disinfected, and the cleaning liquid in the storage tank can be sucked after the suction push handle rises, and the sucked cleaning liquid can be squeezed into the groove of the adjacent split sleeve through the one-way valve after the suction push handle descends, and the suction push handle in the groove rises at the same time, which is convenient for the disinfectant to quickly pass through the one-way valve, and the suction push handle in the groove filled with disinfectant descends, and the disinfectant can be transported into the storage hose and the sampling straw through the delivery pipeline to clean the storage hose and the sampling straw.

[0025] The present invention provides a stem cell sampling method, comprising the following steps:

[0026] S1: The test tube containing the sample can be placed on a test tube rack in the casing by a manipulator or manually. After the sealed door of the casing is closed, the disinfection lamp is turned on, and the four fixed plates are pulled down by the adjusting hydraulic cylinder in the supporting sleeve. The sampling pipette on the fixed plate enters the test tube on the test tube rack. After the sampling pipette is inserted into the sample, the rotating motor on the dividing sleeve drives the worm to rotate, and the rotation of the worm can drive the worm gear meshing with it to rotate, and drive the rotating gear fixedly installed with it to rotate. The rotating gears can rotate synchronously, and the adjacent rotating gears rotate in opposite directions, driving the adjacent threaded sleeves to be lifted and lowered on the plug-in rod. The threaded sleeve can push the suction push handle to rise. At the same time, the plug-in connecting pipe is magnetically connected to the adsorption ring on the conveying pipeline through the adsorption electromagnet, and then the plug-in connecting pipe is plugged into the conveying pipeline;

[0027] S2: After the suction push handle rises, the storage hose can be sucked by plugging the connecting pipe and the conveying pipe. The generated suction can suck the sample in the test tube through the sampling pipette and then enter the storage hose. At the same time, the disinfectant in the storage tank is sucked after the suction push handle in the storage tank rises synchronously with the connecting pipe. The sucked disinfectant is stored in a groove of the dividing sleeve. The other two suction push handles can be lowered. At the same time, the gear on the driving motor is engaged with the gear on the transmission rod. The transmission rod is driven to rotate by the driving motor. After the transmission rod rotates, the locking screw is driven to rotate. After the self-locking block is driven to clamp one end of the storage hose to prevent the sample from entering the conveying pipe, the driving motor is driven to descend by the lifting hydraulic cylinder. After the driving motor is engaged with the gear of the transmission rod, the driving screw is driven to rotate. After the driving frame on the driving screw is driven to descend, the storage hose is driven to deflect to prevent the sample from flowing back. The sample can be retained in the storage hose;

[0028] S3: After the hydraulic cylinder is adjusted to reset, the fixed plate drives the fixed plate to reset under the action of the reset spring. After the adsorption electromagnet is powered off, the plug-in connecting pipe resets under the action of the connecting spring, and the connection with the conveying pipeline is released. The adjusting motor on the casing drives the limit sleeve to rotate, and the fixed plate is switched. The fixed plate drives the sampling test tube after sampling to be located on the other side. The hydraulic cylinder drives the fixed plate to descend, and the separation device or the test tube in the separation device is placed on the other side. At the same time, during the rotation process, the rotating motor starts to drive the threaded sleeve to reset quickly, so that the descending suction push handle rises. At the same time, after the ascending suction push handle descends, it can push the disinfectant in the segmentation sleeve through the one-way valve into the adjacent groove in the segmentation sleeve. Through the rise of the suction push handle, the disinfectant can quickly pass through the one-way valve into the groove. The rotating motor starts to drive the suction push handle with the sample side to descend, and at the same time, the plug-in connecting pipe is plugged into the conveying pipeline again under the action of the adsorption electromagnet.

[0029] S4: Another driving motor drives the transmission rod to rotate. After the transmission rod drives the locking screw to rotate, the clamping of the storage hose is released. The driving motor, under the push of the lifting hydraulic cylinder, can drive the driving screw to rotate, drive the driving frame to rise, and drive the storage hose to tilt, so that the sucked sample can enter the sampling pipette and enter the test tube or separator below;

[0030] S5: After the disinfectant in the groove of the dividing sleeve filled with disinfectant descends under the push of the suction push handle, the disinfectant is transported into the storage hose through the delivery pipe, and enters the sampling straw through the storage hose. After the inner walls of the storage hose and the sampling straw are cleaned and disinfected, the used disinfectant enters the collection tank below for collection. When the outer surface of the sampling straw is cleaned, the sampling straw is inserted into the cleaning tank when it descends, and the disinfectant is sucked by the water pump and sprayed on the outer surface of the sampling straw through the cleaning nozzle, completing the cleaning of the outer surface of the sampling straw. The used disinfectant is collected through the outlet pipe.

[0031] The beneficial effects of the present invention are:

[0032] 1. By setting an adjustment device, sampling can be performed automatically. By adjusting the lifting of the hydraulic cylinder, multiple fixed plates can be driven to descend, so that the sampling pipettes on the fixed plates can be inserted into the test tubes containing samples, which is convenient for sampling. The sampling pipettes on another fixed plate are lowered into the separation equipment, which is convenient for transporting the sampled samples. At the same time, the suction pipettes that have been transported can be lowered for easy cleaning, thereby improving work efficiency. By adjusting the rotation of the motor, the fixed plates can be driven to complete the switching work, thereby reducing labor input and solving the problems of existing manual sampling, reducing work efficiency, and manual mechanical operations that are prone to fatigue. Finally, manual sampling is prone to sample contamination, affecting the final experimental results.

[0033] 2. By setting up a sampling device, automatic sampling can be performed. After the suction push handle on the sampling side rises, the suction force generated can be transmitted to the sampling pipette to realize automatic sampling. The rotation of the driving screw can drive the driving frame to rise and fall, and drive the storage hose to deflect, so that the sucked sample will not flow back. At the same time, it can be quickly transported when the sample needs to be transported. The rotation of the locking screw can drive the self-locking block to move relatively, which is convenient for sealing one end of the storage hose to prevent the sucked sample from entering the delivery pipeline. At the same time, when suction is needed, the self-locking block can be opened quickly to facilitate the circulation of gas, so that one of the split sleeves has suction force and the other has driving force, which is convenient for sucking and transporting samples, and solves the technical problems of existing manual sampling and reduced work efficiency.

[0034] 3. By arranging a cleaning device, the storage hose and the suction straw can be automatically cleaned after use. By suctioning with a water pump, the disinfectant can be transported into the cleaning nozzle to clean the outer surface of the sampling straw. The cleaning liquid in the storage tank can be sucked by the rising of the suction push handle. After the suction push handle is lowered, the sucked cleaning liquid can be squeezed through the one-way valve into the groove of the adjacent dividing sleeve. At the same time, the suction push handle in the groove rises to facilitate the disinfectant to pass through the one-way valve quickly. The suction push handle in the groove filled with the disinfectant descends, and the disinfectant can be transported into the storage hose and the sampling straw through the delivery pipeline to clean the storage hose and the sampling straw. Therefore, the storage hose and the sampling straw can be automatically cleaned and disinfected, which solves the technical problems of existing manual sampling, reduced work efficiency, and the need to disinfect and clean the sampling tools, which is cumbersome to operate. BRIEF DESCRIPTION OF THE DRAWINGS

[0035] Figure 1 A schematic diagram of a stem cell sampling device proposed by the present invention;

[0036] Figure 2A three-dimensional diagram of a supporting sleeve structure of a stem cell sampling device proposed by the present invention;

[0037] Figure 3 A three-dimensional diagram of a disinfection lamp structure of a stem cell sampling device proposed by the present invention;

[0038] Figure 4 A three-dimensional diagram of the regulating motor structure of a stem cell sampling device proposed by the present invention;

[0039] Figure 5 A three-dimensional diagram of a storage hose structure of a stem cell sampling device proposed by the present invention;

[0040] Figure 6 A three-dimensional diagram of the structure of an adjusting hydraulic cylinder of a stem cell sampling device proposed by the present invention;

[0041] Figure 7 A three-dimensional diagram of a fixing plate structure of a stem cell sampling device proposed by the present invention;

[0042] Figure 8 A three-dimensional diagram of a driving motor structure of a stem cell sampling device proposed by the present invention;

[0043] Fig. 9 A three-dimensional diagram of a self-locking block structure of a stem cell sampling device proposed by the present invention;

[0044] Fig.10 A three-dimensional diagram of a delivery pipeline structure of a stem cell sampling device proposed by the present invention;

[0045] Fig.11 A three-dimensional diagram of a split sleeve structure of a stem cell sampling device proposed by the present invention;

[0046] Fig.12 A three-dimensional diagram of a threaded sleeve structure of a stem cell sampling device proposed by the present invention;

[0047] Fig.13 A three-dimensional diagram of a rotating gear structure of a stem cell sampling device proposed by the present invention;

[0048] Fig.14 A three-dimensional diagram of a storage tank structure of a stem cell sampling device proposed by the present invention;

[0049] Fig.15 This is a three-dimensional diagram of the cleaning nozzle structure of a stem cell sampling device proposed by the present invention.

[0050] In the figure: 1. casing; 11. test tube rack; 12. disinfection lamp; 2. support sleeve; 21. adjusting hydraulic cylinder; 22. lifting plate; 3. limit sleeve; 31. adjusting motor; 32. fixing plate; 33. reset spring; 4. sampling pipette; 41. storage hose; 42. delivery pipeline; 5. driving screw; 51. driving frame; 52. locking screw; 53. self-locking block; 54. transmission rod; 55. lifting hydraulic cylinder; 56. driving motor; 6. dividing sleeve; 61. plug-in rod; 62. threaded sleeve; 63. rotating gear; 64. suction push handle; 65. rotating motor; 66. worm; 67. worm wheel; 7. plug-in connecting pipe; 71. adsorption electromagnet; 72. connecting spring; 8. storage tank; 81. one-way valve; 82. cleaning tank; 83. cleaning nozzle; 84. water pump; 85. collecting tank. DETAILED DESCRIPTION

[0051] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments.

[0052] Reference Figure 1-Figure 15 A stem cell sampling device includes a housing 1, a test tube rack 11 installed in the housing 1, a disinfection lamp 12, an adjustment device, a sampling device and a cleaning device.

[0053] The test tube rack 11 is fixedly mounted on the inner bottom wall of the casing 1 , and can support the test tubes containing samples. The disinfection lamp 12 is fixedly mounted on the inner top wall of the casing 1 , and can disinfect the environment inside the casing 1 .

[0054] like Figure 2-Figure 10 As shown, in order to perform automatic sampling, the adjusting device is located inside the housing 1 and adjusts the position of the sampling device. The adjusting device includes an adjusting hydraulic cylinder 21. The lifting and lowering of the adjusting hydraulic cylinder 21 drives the sampling device to lift and lower.

[0055] Specifically, in order to speed up the sampling speed, the adjustment device includes a support sleeve 2, which is fixedly installed on the inner wall of the casing 1. The inner wall of the support sleeve 2 is fixedly installed with one end of the piston rod of the adjusting hydraulic cylinder 21, and a lifting plate 22 is fixedly installed on one end of the piston rod of the adjusting hydraulic cylinder 21.

[0056] Specifically, in order to drive the sampling device to lift and rotate, the upper surface of the supporting sleeve 2 is rotatably connected to a limiting sleeve 3 with a gear ring through a bearing, and an adjusting motor 31 is fixedly installed on the inner wall of the casing 1. One end of the output shaft of the adjusting motor 31 drives one end of the limiting sleeve 3 to rotate through a gear. A fixing plate 32 distributed in a ring array is slidably inserted into the inner wall of the limiting sleeve 3. The upper surface of the fixing plate 32 contacts the lower surface of the lifting plate 22, and a reset spring 33 is fixedly installed on the lower surface of the fixing plate 32. One end of the reset spring 33 is fixedly installed on the inner wall of the limiting sleeve 3.

[0057] Specifically, in order to perform automatic sampling, the sampling pipette 4 is threadedly connected to one end of the fixed plate 32. The sampling pipette 4 is threadedly connected to facilitate disassembly and replacement. One end of the sampling pipette 4 is fixedly connected to one end of the storage hose 41. One end of the sampling pipette 4 is plugged into the storage hose 41 for easy disassembly. One end of the storage hose 41 is fixedly connected to a delivery pipe 42 with an adsorption ring. The delivery pipe 42 is fixedly installed on the upper surface of the limiting sleeve 3 through a bracket.

[0058] like Figure 11-13 As shown, in order to automatically perform sampling, the sampling device is located on the outer surface of the regulating device, and automatically samples the test tube containing the sample. The sampling device includes a sampling mechanism, a driving mechanism and a suction mechanism. The sampling mechanism includes a sampling pipette 4 and a storage hose 41 with a collar. The sampling pipette 4 sucks the sample in the test tube containing the sample into the storage hose 41. The driving mechanism includes a driving frame 51. The lifting and lowering of the driving frame 51 drives the storage hose 41 to deflect. The suction mechanism includes a suction push handle 64. The lifting and lowering of the suction push handle 64 drives the sampling pipette 4 to perform suction.

[0059] Specifically, in order to drive the storage hose 41 to deflect and seal one end, the driving mechanism also includes a driving screw 5 with a gear, the driving screw 5 is rotatably connected to the outer surface of the fixed plate 32 through a bearing, a driving frame 51 is threadedly connected to one end of the driving screw 5, both ends of the driving frame 51 are hinged to the outer surface of the sleeve of the storage hose 41 through a pin shaft, the outer surface of the driving frame 51 and the outer surface of the fixed plate 32 are slidably plugged through a limit block, the outer surface of the driving frame 51 is rotatably connected to a locking screw 52 through a bearing, and the outer surface of the locking screw 52 is threaded A self-locking block 53 is connected with the groove, one end of the self-locking block 53 is slidably inserted into one end of the limit rod of the driving frame 51, and the outer surface of the driving frame 51 is rotatably connected to a transmission rod 54 with a gear through a bearing, and one end of the transmission rod 54 drives the locking screw 52 to rotate through a bevel gear set. A lifting hydraulic cylinder 55 is fixedly installed on the outer side of the support sleeve 2 through a connecting frame, and a driving motor 56 is fixedly installed on the piston rod of the lifting hydraulic cylinder 55 through a connecting plate. One end of the output shaft of the driving motor 56 drives the transmission rod 54 and the driving screw 5 to rotate through gears.

[0060] Specifically, in order to automatically suck and transport samples, the suction mechanism also includes a split sleeve 6, which is fixedly installed on the inner bottom wall of the casing 1, and a plug-in rod 61 is fixedly installed on the inner top wall of the casing 1. The outer surface of the plug-in rod 61 is slidably sleeved with a threaded sleeve 62. In order to drive the threaded sleeve 62 to rise and fall, the upper surface of the split sleeve 6 is rotatably connected to a rotating gear 63 through a bearing, and adjacent rotating gears 63 are meshed. One end of the threaded sleeve 62 is threadedly connected to the inner wall of the rotating gear 63, and the outer surface of the suction push handle 64 is rotatably connected to one end of the threaded sleeve 62 through a bearing.

[0061] Specifically, in order to drive the rotating gear 63 to rotate, a rotating motor 65 is fixedly installed on the outer surface of the dividing sleeve 6, a worm 66 is fixedly installed on one end of the output shaft of the rotating motor 65, and a worm wheel 67 is fixedly installed on the outer surface of a rotating gear 63, and the worm 66 is meshed with the worm wheel 67.

[0062] Specifically, in order to facilitate the delivery of the suction force and the driving force to the delivery pipe 42, one end of the split sleeve 6 is slidably plugged with a plug-in connecting pipe 7, and an adsorption electromagnet 71 is fixedly installed on the outer surface of the plug-in connecting pipe 7. The outer surface of the adsorption electromagnet 71 is magnetically connected to the adsorption ring of the delivery pipe 42, and a connecting spring 72 is fixedly installed on the outer surface of the plug-in connecting pipe 7, and one end of the connecting spring 72 is fixedly installed on one end of the split sleeve 6.

[0063] like Figure 14-15 As shown, in order to clean the storage hose 41 and the sampling pipette 4 , a cleaning device is located inside the housing 1 , and cleans the sampling pipette 4 and the storage hose 41 . The cleaning device includes a cleaning nozzle 83 , and the cleaning nozzle 83 flushes the outer surface of the sampling pipette 4 .

[0064] Specifically, in order to automatically clean the interior of the storage hose 41 and the sampling suction pipe 4, the cleaning device also includes a storage tank 8, which is fixedly mounted on the upper surface of the casing 1, and the lower surface of the storage tank 8 is fixedly connected to the inner wall of the split sleeve 6 through a connecting pipe with a control valve, and a one-way valve 81 is fixedly mounted on the inner wall of the split sleeve 6, and a cleaning trough body 82 with a water outlet pipe is fixedly mounted on the inner wall of the casing 1, and the inner wall of the cleaning trough body 82 is fixedly mounted to the outer surface of the cleaning nozzle 83, and a water pump 84 is fixedly mounted on the inner wall of the casing 1, and the water inlet end of the water pump 84 is fixedly connected to the outer surface of the storage tank 8, and the water outlet end of the water pump 84 is fixedly connected to the outer surface of the cleaning nozzle 83, and a collecting trough body 85 with a water outlet pipe is fixedly mounted on the inner bottom wall of the casing 1, and the water outlet pipe of the collecting trough body 85 is fixedly connected to the water outlet pipe of the cleaning trough body 82.

[0065] like Figure 1-Figure 15As shown, a sampling method of a stem cell sampling device proposed by the present invention comprises the following steps:

[0066] S1: The test tube containing the sample can be placed on a test tube rack 11 in the casing 1 by a manipulator or manually. After the sealing door of the casing 1 is closed, the disinfection lamp 12 is turned on, and the four fixed plates 32 are pulled down by the adjusting hydraulic cylinder 21 in the supporting sleeve 2. The sampling pipette 4 on the fixed plate 32 enters the test tube on the test tube rack 11. After the sampling pipette 4 is inserted into the sample, the rotating motor 65 on the dividing sleeve 6 drives the worm 66 to rotate, and the rotation of the worm 66 can drive the worm gear 67 meshing with it to rotate, and drive the rotating gear 63 fixedly installed therewith to rotate. The rotating gear 63 can rotate synchronously, and the adjacent rotating gears 63 rotate in opposite directions, driving the adjacent threaded sleeves 62 to be lifted and lowered on the plug-in rod 61, and the threaded sleeve 62 can push the suction push handle 64 to rise. At the same time, after the plug-in connecting pipe 7 is magnetically connected to the adsorption ring on the conveying pipe 42 through the adsorption electromagnet 71, the plug-in connecting pipe 7 is plugged into the conveying pipe 42;

[0067] S2: After the suction push handle 64 rises, the storage hose 41 can be sucked by plugging the connecting pipe 7 and the delivery pipe 42. The generated suction can suck the sample in the test tube through the sampling pipette 4 and then enter the storage hose 41. At the same time, the suction push handle 64 in the storage tank 8 rises synchronously with the connecting pipe to suck the disinfectant in the storage tank 8. The sucked disinfectant is stored in a groove of the dividing sleeve 6. The other two suction push handles 64 can be lowered. At the same time, the gear on the driving motor 56 is meshed with the gear on the transmission rod 54. The transmission rod 54 is driven to rotate by the driving motor 56. After the transmission rod 54 rotates, the locking screw 52 is driven to rotate, and the self-locking block 53 is driven to clamp one end of the storage hose 41 to prevent the sample from entering the delivery pipe 42. After the driving motor 56 is driven to descend by the lifting hydraulic cylinder 55, the driving motor 56 is meshed with the gear of the transmission rod 54, and the driving screw 5 is driven to rotate. After the driving frame 51 on the driving screw 5 is driven to descend, the storage hose 41 is driven to deflect to prevent the sample from flowing back, and the sample can be retained in the storage hose 41;

[0068] S3: After the hydraulic cylinder 21 is adjusted to reset, the fixing plate 32 is driven to reset by the reset spring 33. After the adsorption electromagnet 71 is powered off, the plug-in connecting tube 7 is reset by the connecting spring 72 to release the connection with the delivery pipeline 42. The adjusting motor 31 on the casing 1 drives the limit sleeve 3 to rotate, and the fixing plate 32 is switched. The fixed plate 32 drives the sampled test tube to be located on the other side. The hydraulic cylinder 21 drives the fixing plate 32 to descend, and the separation device or the test tube in the separation device is placed on the other side. At the same time, during the rotation, During the process, the rotating motor 65 is started, driving the threaded sleeve 62 to quickly reset, so that the descending suction push handle 64 is raised, and at the same time, after the ascending suction push handle 64 is lowered, it can push the disinfectant in the segmentation sleeve 6 through the one-way valve 81 into the adjacent groove in the segmentation sleeve 6. Through the rise of the suction push handle 64, the disinfectant can quickly pass through the one-way valve 81 into the groove, and the rotating motor 65 is started, driving the suction push handle 64 with the sample side to descend, and at the same time, the plug-in connecting pipe 7 is plugged into the delivery pipe 42 again under the action of the adsorption electromagnet 71;

[0069] S4: Another driving motor 56 drives the transmission rod 54 to rotate. After the transmission rod 54 drives the locking screw 52 to rotate, the clamping of the storage hose 41 is released. The driving motor 56, under the push of the lifting hydraulic cylinder 55, can drive the driving screw 5 to rotate, drive the driving frame 51 to rise, and drive the storage hose 41 to tilt, so that the sucked sample can enter the sampling pipette 4 and enter the test tube or separator below;

[0070] S5: After the disinfectant in the groove of the dividing sleeve 6 filled with disinfectant is lowered under the push of the suction push handle 64, the disinfectant is transported into the storage hose 41 through the delivery pipe 42, and enters the sampling straw 4 through the storage hose 41. After the inner walls of the storage hose 41 and the sampling straw 4 are cleaned and disinfected, the used disinfectant enters the collecting tank 85 below for collection. When the outer surface of the sampling straw 4 is cleaned, the sampling straw 4 is inserted into the cleaning tank 82 when it descends, and the disinfectant is sucked by the water pump 84, and the disinfectant is sprayed on the outer surface of the sampling straw 4 through the cleaning nozzle 83, completing the cleaning of the outer surface of the sampling straw 4, and the used disinfectant is collected through the outlet pipe.

[0071] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical scheme and inventive concept of the present invention within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A stem cell sampling device, comprising a housing (1), characterized in that: It also includes a test tube rack (11), a disinfection lamp (12), an adjustment device, a sampling device and a cleaning device installed in the housing (1); The adjusting device is located inside the housing (1) and adjusts the position of the sampling device. The adjusting device comprises an adjusting hydraulic cylinder (21). The lifting and lowering of the adjusting hydraulic cylinder (21) drives the sampling device to be lifted and lowered. The sampling device is located on the outer surface of the regulating device and automatically samples the test tube containing the sample. The sampling device comprises a sampling mechanism, a driving mechanism and a suction mechanism. The sampling mechanism comprises a sampling pipette (4) and a storage hose (41) with a collar. The sampling pipette (4) sucks the sample in the test tube containing the sample into the storage hose (41). The driving mechanism comprises a driving frame (51). The lifting and lowering of the driving frame (51) drives the storage hose (41) to deflect. The suction mechanism comprises a suction push handle (64). The lifting and lowering of the suction push handle (64) drives the sampling pipette (4) to suction. The cleaning device is located inside the housing (1) and cleans the sampling pipette (4) and the storage hose (41). The cleaning device comprises a cleaning nozzle (83) and the cleaning nozzle (83) flushes the outer surface of the sampling pipette (4).

2. A stem cell sampling device and a sampling method thereof according to claim 1, characterized in that: The adjusting device comprises a supporting sleeve (2), wherein the supporting sleeve (2) is fixedly mounted on the inner wall of the casing (1), the inner wall of the supporting sleeve (2) is fixedly mounted on one end of the piston rod of the adjusting hydraulic cylinder (21), and a lifting plate (22) is fixedly mounted on one end of the piston rod of the adjusting hydraulic cylinder (21).

3. A stem cell sampling device according to claim 2, characterized in that: The upper surface of the support sleeve (2) is rotatably connected to a limiting sleeve (3) with a gear ring via a bearing; an adjusting motor (31) is fixedly mounted on the inner wall of the housing (1); one end of the output shaft of the adjusting motor (31) drives one end of the limiting sleeve (3) to rotate via a gear; a fixing plate (32) distributed in a ring array is slidably inserted into the inner wall of the limiting sleeve (3); the upper surface of the fixing plate (32) contacts the lower surface of the lifting plate (22); a return spring (33) is fixedly mounted on the lower surface of the fixing plate (32); one end of the return spring (33) is fixedly mounted on the inner wall of the limiting sleeve (3).

4. A stem cell sampling device according to claim 3, characterized in that: The sampling pipette (4) is threadedly connected to one end of the fixing plate (32), one end of the sampling pipette (4) is fixedly connected to one end of the storage hose (41), one end of the storage hose (41) is fixedly connected to a delivery pipe (42) with an adsorption ring, and the delivery pipe (42) is fixedly mounted on the upper surface of the limiting sleeve (3) through a bracket.

5. A stem cell sampling device according to claim 4, characterized in that: The driving mechanism further comprises a driving screw (5) with a gear, the driving screw (5) being rotatably connected to the outer surface of the fixing plate (32) via a bearing, the driving frame (51) being threadedly connected to one end of the driving screw (5), the two ends of the driving frame (51) being hinged to the outer surface of the sleeve ring of the storage hose (41) via a pin shaft, the outer surface of the driving frame (51) being slidably plugged with the outer surface of the fixing plate (32) via a limit block, the outer surface of the driving frame (51) being rotatably connected to a locking screw (52) via a bearing, the outer surface of the locking screw (52) being threadedly connected to a self-locking block (53), the One end of the self-locking block (53) is slidably plugged into one end of the limit rod of the driving frame (51); the outer surface of the driving frame (51) is rotatably connected to a transmission rod (54) with a gear through a bearing; one end of the transmission rod (54) drives the locking screw (52) to rotate through a bevel gear set; a lifting hydraulic cylinder (55) is fixedly installed on the outer side surface of the supporting sleeve (2) through a connecting frame; a driving motor (56) is fixedly installed on the piston rod of the lifting hydraulic cylinder (55) through a connecting plate; one end of the output shaft of the driving motor (56) drives the transmission rod (54) and the driving screw (5) to rotate through a gear.

6. A stem cell sampling device according to claim 5, characterized in that: The suction mechanism also includes a split sleeve (6), the split sleeve (6) is fixedly mounted on the inner bottom wall of the casing (1), a plug-in rod (61) is fixedly mounted on the inner top wall of the casing (1), a threaded sleeve (62) is slidably sleeved on the outer surface of the plug-in rod (61), the upper surface of the split sleeve (6) is rotatably connected to a rotating gear (63) via a bearing, adjacent rotating gears (63) are meshed, one end of the threaded sleeve (62) is threadedly connected to the inner wall of the rotating gear (63), and the outer surface of the suction push handle (64) is rotatably connected to one end of the threaded sleeve (62) via a bearing.

7. A stem cell sampling device according to claim 6, characterized in that: A rotating motor (65) is fixedly mounted on the outer surface of the split sleeve (6), a worm (66) is fixedly mounted on one end of the output shaft of the rotating motor (65), a worm wheel (67) is fixedly mounted on the outer surface of one of the rotating gears (63), and the worm (66) meshes with the worm wheel (67).

8. A stem cell sampling device according to claim 7, characterized in that: One end of the split sleeve (6) is slidably plugged with a plug-in connecting pipe (7), an outer surface of the plug-in connecting pipe (7) is fixedly mounted with an adsorption electromagnet (71), the outer surface of the adsorption electromagnet (71) is magnetically connected to the adsorption ring of the conveying pipe (42), and an outer surface of the plug-in connecting pipe (7) is fixedly mounted with a connecting spring (72), one end of the connecting spring (72) is fixedly mounted with one end of the split sleeve (6).

9. A stem cell sampling device according to claim 8, characterized in that: The cleaning device further comprises a storage tank (8), the storage tank (8) being fixedly mounted on the upper surface of the housing (1), the lower surface of the storage tank (8) being fixedly connected to the inner wall of the split sleeve (6) via a connecting pipe with a control valve, a one-way valve (81) being fixedly mounted on the inner wall of the split sleeve (6), a cleaning tank body (82) with a water outlet pipe being fixedly mounted on the inner wall of the housing (1), the inner wall of the cleaning tank body (82) being connected to the outer wall of the cleaning nozzle (83) The housing (1) is fixedly mounted on the inner wall of the housing (1), a water pump (84) is fixedly mounted, a water inlet end of the water pump (84) is fixedly connected to the outer surface of the storage tank (8), a water outlet end of the water pump (84) is fixedly connected to the outer surface of the cleaning nozzle (83), and a collecting tank body (85) with a water outlet pipe is fixedly mounted on the inner bottom wall of the housing (1), the water outlet pipe of the collecting tank body (85) is fixedly connected to the water outlet pipe of the cleaning tank body (82).

10. A sampling method of a stem cell sampling device, using the stem cell sampling device according to any one of claims 1 to 9, characterized in that: S1: A test tube containing a sample can be placed on a test tube rack (11) in the housing (1) by a manipulator or manually. After the sealed door of the housing (1) is closed, the disinfection lamp (12) is turned on, and the four fixed plates (32) are pulled down by the adjustment hydraulic cylinder (21) in the support sleeve (2). The sampling pipette (4) on the fixed plate (32) enters the test tube on the test tube rack (11). After the sampling pipette (4) is inserted into the sample, the rotating motor (65) on the dividing sleeve (6) drives the worm (66) to rotate, and the rotation of the worm (66) can drive the worm (66) to rotate. The meshing worm gear (67) rotates, driving the rotating gear (63) fixed thereto to rotate, and the rotating gear (63) can rotate synchronously. The adjacent rotating gears (63) rotate in opposite directions, driving the adjacent threaded sleeves (62) to move up and down on the plug-in rod (61). The threaded sleeves (62) can push the suction push handle (64) to move up. At the same time, the plug-in connecting pipe (7) is magnetically connected to the adsorption ring on the conveying pipe (42) through the adsorption electromagnet (71), and then the plug-in connecting pipe (7) and the conveying pipe (42) are plugged in. S2: After the suction push handle (64) rises, it can suck the storage hose (41) by plugging the connecting pipe (7) and the delivery pipe (42). The generated suction force can suck the sample in the test tube through the sampling pipette (4) and then enter the storage hose (41). At the same time, the suction push handle (64) in the storage tank (8) rises synchronously with the connecting pipe to suck the disinfectant in the storage tank (8). The sucked disinfectant is stored in a groove of the dividing sleeve (6). The other two suction push handles (64) can be lowered. At the same time, the gear on the driving motor (56) is meshed with the gear on the transmission rod (54). The driving motor (56) drives the transmission rod (54) to rotate. After the transmission rod (54) rotates, the locking screw (52) is driven to rotate, and the self-locking block (53) is driven to clamp one end of the storage hose (41) to prevent the sample from entering the delivery pipe (42). After the driving motor (56) is driven to descend by the lifting hydraulic cylinder (55), the driving motor (56) is meshed with the gear of the transmission rod (54), and the driving screw (5) is driven to rotate. After the driving frame (51) on the driving screw (5) is driven to descend, the storage hose (41) is driven to deflect to prevent the sample from flowing back, and the sample can be retained in the storage hose (41); S3: After the adjusting hydraulic cylinder (21) is reset, the fixing plate (32) is driven to reset under the action of the reset spring (33). After the adsorption electromagnet (71) is powered off, the plug-in connecting pipe (7) is reset under the action of the connecting spring (72), and the connection with the conveying pipe (42) is released. The adjusting motor (31) on the housing (1) drives the limit sleeve (3) to rotate, and the fixing plate (32) is switched. The fixing plate (32) drives the sampled test tube to be located on the other side after sampling. The adjusting hydraulic cylinder (21) drives the fixing plate (32) to descend, and the separation device or the test tube in the separation device is placed on the other side. At the same time, the rotating During the process, the rotating motor (65) is started, driving the threaded sleeve (62) to quickly reset, so that the descending suction push handle (64) is raised. At the same time, after the ascending suction push handle (64) is lowered, it can push the disinfectant in the split sleeve (6) through the one-way valve (81) into the adjacent groove in the split sleeve (6). Through the rise of the suction push handle (64), the disinfectant can quickly pass through the one-way valve (81) and enter the groove. The rotating motor (65) is started, driving the suction push handle (64) with the sample side to descend, and at the same time, the plug-in connecting pipe (7) is plugged into the conveying pipe (42) again under the action of the adsorption electromagnet (71); S4: Another driving motor (56) drives the transmission rod (54) to rotate. After the transmission rod (54) drives the locking screw (52) to rotate, the clamping of the storage hose (41) is released. The driving motor (56) is driven by the lifting hydraulic cylinder (55) to drive the driving screw (5) to rotate, and after driving the driving frame (51) to rise, the storage hose (41) is driven to tilt, so that the sucked sample can enter the sampling pipette (4) and enter the test tube or separator below. S5: After the disinfectant in the groove of the dividing sleeve (6) containing the disinfectant is lowered under the push of the suction push handle (64), the disinfectant is transported into the storage hose (41) through the transport pipe (42), and then enters the sampling pipette (4) through the storage hose (41). After the inner walls of the storage hose (41) and the sampling pipette (4) are cleaned and disinfected, the used disinfectant enters the collecting tank (85) below for collection. When the outer surface of the sampling pipette (4) is cleaned, the sampling pipette (4) is inserted into the cleaning tank (82) when it descends, and the disinfectant is sucked by the water pump (84). The disinfectant is sprayed on the outer surface of the sampling pipette (4) through the cleaning nozzle (83), and the cleaning of the outer surface of the sampling pipette (4) is completed. The used disinfectant is collected through the water outlet pipe.

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