The application discloses a preparation method of MICP combined with molasses reinforced
calcareous sand. The preparation method comprises the following steps: activating and culturing mineralization
bacteria, preparing a molasses culture medium by using waste molasses, inoculating the activated mineralization
bacteria liquid on the molasses culture medium, and after the OD600 value of the mineralization
bacteria in the molasses culture medium is between 1.0 and 1.5, injecting the molasses culture medium containing the mineralization bacteria into the
calcareous sand for reinforcement, so that the reinforced
calcareous sand is obtained. The preparation method of the MICP combined with molasses reinforced calcareous sand disclosed by the application directly uses waste molasses as a
carbon source to culture mineralization bacteria, greatly reduces the preparation cost, and is green and environment-friendly in the preparation process; by adding sugarcane residue with a proper proportion and length in the waste molasses, the viscous
sugar silk and the sugarcane residue are uniformly distributed in the reinforced calcareous sand, so that the generation amount of the microbial-induced
calcium carbonate crystal is improved, and the prepared calcareous sand has more excellent reinforcement effect, and the unconfined
compressive strength can reach 2 MPa at most.