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Efficient liquid-phase conic chromatographic column

A technology of high performance liquid chromatography and liquid chromatography, which is applied in the field of high performance liquid chromatography columns, and can solve the problems of not having a tapered high performance liquid chromatography preparation column, etc.

Inactive Publication Date: 2002-08-28
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no report on the conical HPLC preparative column

Method used

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  • Efficient liquid-phase conic chromatographic column
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  • Efficient liquid-phase conic chromatographic column

Examples

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Effect test

Embodiment 1

[0019] Example 1: The inner diameter of the inlet of the conical liquid chromatography preparative column is 20 mm, the inner diameter of the outlet is 7 mm, and the length is 5 cm. according to figure 1 The two ends of the column shown are bolt-type column head 1 and column tail 3, which are matched with the upper and lower ends of the column body 2 with thread 8 and nut 7 respectively; the inner surfaces of column head 1 and column tail 3 are concave into tapered guide grooves 4; O-ring 9, distribution plate 5, porous sieve plate 6 under column head 1; O-ring 9 and porous sieve plate 6 above column tail 3. according to image 3As shown, on the upper part of the distribution plate 5, there is a concentric circular liquid flow collection channel at 3 / 5 of the radius, and there are 12 radial liquid flow channels in the circle, and there is a seepage hole in the center of every other channel, without seepage. The channel of the liquid hole is divided into two channels at the i...

Embodiment 2

[0022] Example 2: The inner diameter of the inlet of the conical liquid chromatography preparative column is 800 mm, the inner diameter of the outlet is 280 mm, and the length is 25 cm. according to figure 2 The two ends of the shown cylinder 2 are respectively equipped with a column head 1 and a column tail 3 composed of flanges 10; the flange 10 is fastened by bolts 11, and there is a sealing washer 12 in the middle, and the inner surfaces of the column head 1 and the column tail 3 are concave into cones. There is a distribution plate 5 and a porous sieve plate 6 below the column head 1; a porous sieve plate 6 is arranged above the column tail 3. according to image 3 As shown, when the inner diameter 2R of the cylinder inlet is relatively large, the number of seepage holes and concentric circles can be increased as required, or any other form of distribution plate can be used. The function of the distribution plate is to distribute the sample evenly over the entire colum...

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Abstract

A high-efficiency cone-shaped preparation column for liquid chromatography is composed of head body and tail of column with inside diameter of head inlet larger than inside diameter of tail outlet. Cone-shaped liquid flow guide channel, distribution disk and sieve plate constitute the liquid flow distributing system located on the head of the column. The distribution disk consists of multiple radial liquid flow channels concentric liquid collecting channel and liquid exuding holes. Inside diameter of the inlet is 2R=10mm-2000mm and inside diameter of the outlet is 2r=3mm-1800mm, the length ofthe column is L=5cm-100cm. Angle of cone is is in range of 1-20 degrees. Various chromatographic separation media can be filled into the cone. The ivnented cone-shaped preparation column can increasecolumn efficiency and specimen loading and its diluting effect of separated components is lower than that of cylindrical chromatographic column.

Description

technical field [0001] The invention belongs to a high-efficiency liquid chromatographic column for preparation and separation, and is suitable for fields requiring pure products such as petrochemical, fine chemical, biotechnology, and pharmacy, especially for biomacromolecules, natural active ingredients, etc. that require high purification Preparation and separation of complex samples. Background technique [0002] Chromatography originated in 1906, when Russian botanist M.S. Tswett separated plant pigments by chromatography for the first time. Early preparative liquid chromatography was mainly used for the separation and purification of organic compounds and petroleum products, and the requirements for product purity were not high. Therefore, the past view was that column efficiency was not the main problem in preparative column chromatography, and what people really cared about was the unit. time production. However, since the 1980s, the development of pharmaceuticals ...

Claims

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Application Information

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IPC IPC(8): B01D15/08G01N30/60
CPCG01N30/6065
Inventor 关亚风马继平陈令新
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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