Scalable multiple-proportion dilution microfluidic chip as well as preparation method and dilution method of microfluidic chip

Abstract

The invention provides a scalable multiple-proportion dilution microfluidic chip. The microfluidic chip is composed of a cover sheet and a substrate, the upper layer is the cover sheet provided with asquare wave micro flow channel network structure, the lower layer is the flat substrate, and the upper-layer cover sheet and the lower-layer substrate form a closed micro flow channel network throughbonding; two sample inlets and N sample outlets located in both ends of the micro flow channel are connected with soft guide pipes outside, wherein N is an integer >= 1, and N outlets represent a dilution level of a liquid sample to be diluted; a reagent sample to be diluted and a diluent in a micro-syringe are added into the sample inlets through a micro injection pump under a constant positivepressure, respectively, and the soft guide pipes at the sample outlets are connected with a collection device of a sample after multiple-proportion dilution to form the microfluidic chip; each sampleinlet is provided with one filter tank, and the interior of each filter tank is provided with a row of same rectangular stop blocks with a certain interval; each-level dilution module is composed of amixed flow channel and a connecting flow channel, and lengths of the mixed flow channel and the connecting channel are fixed; and rectangular baffles arranged in a staggered manner are arranged at the corner of the interior of the square wave mixed flow channel in each-level dilution zone.

Description

technical field [0001] The invention relates to the technical field of chemical analysis, in particular to an expandable multiple ratio dilution microfluidic chip, a preparation method and a dilution method. Background technique [0002] In recent years, with the rapid development of Micro Total Analysis System (μTAS), microfluidic chip, as a new type of device, has been widely used in biochemistry due to its advantages of small size, fast response and high integration. Analysis, chemical synthesis and clinical testing and other fields. Among them, in the mixing operation of solutions in cell analysis and biochemical experiments, it is often necessary to dilute or quickly mix specific medicinal solutions in a certain proportion. At present, beakers, test tubes, volumetric flasks, etc. are often used for the dilution of large-dose sample solutions to proportion the reagents required for dilution. For the dilution of small-dose sample solutions, the sample solution is often d...

AI Technical Summary

Problems solved by technology

However, since the mixing channel of the chip is serpentine, the mixing of fluids in the channel mainly depends on the diffusion between molecules. In order to completely mix the sample solution to be diluted with the diluent, a long mixing channel is required, resulting in an excessively large chip area. Moreover, the concentration gradient distribution is not fine enough to meet the large-scale dilution of a solution.

The network structure of the chip is complex, and it is difficult to expand it according to the dilution factor of the sample

Moreover, these microfluidic chips fail to consider the problem of channel blockage caused by impurities in the sample solution and macromolecular particles, so they are subject to many limitations in practical applications.

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