Portable non-power-source microfluidic cell separation chip

Abstract

The invention discloses a portable non-power-source microfluidic cell separation chip. The chip comprises a substrate and a cover plate covering the substrate. The substrate comprises an input opening, a flow guiding region, a separating region and a collection opening in sequence from the input end to the output end. The input opening is used for dropwise adding body fluid with cells to be separated, the flow guiding region is used for guiding the body fluid with the cells to be separated to the separating region, the separating region is used for separating the cells, the collection opening is used for collecting the cells separated out in the separating region, the flow guiding region and the separating region are provided with a plurality of regularly-distributed columnar protrusions, the height of the columnar protrusions is 15-50 micrometers, the diameter of the columnar protrusions is 10-50 micrometers, the intervals between the columnar protrusions in the flow guiding region are 15-25 micrometers, and the intervals between the columnar protrusions in the separating region are 2-15 micrometers. The cell separation chip is powered through the capillary action force, does not need a power source, and is convenient to carry and manufacture and easy to operate and use.

Description

technical field [0001] The invention belongs to the field of microfluidic chips, and in particular relates to a cell separation chip without a power source based on a filtration method. Background technique [0002] In recent decades, microfluidic technology has developed rapidly in analytical chemistry, biological detection, clinical medicine and other application fields, and has replaced some traditional application methods. For example, traditional cell analysis and sorting generally use flow cytometry, which is fast, high-precision, and accurate, but requires complex and expensive equipment, and the detection cycle is long, so it is only suitable for large hospitals and laboratories . [0003] Microfluidics uses integrated and miniaturized microfluidic chips, which can quickly detect and analyze samples in a short time with only a small amount of samples. Compared with traditional methods, the whole process is faster, more efficient and environmentally friendly. [00...

AI Technical Summary

Problems solved by technology

[0008] The above methods can achieve efficient particle separation and cell separation, but they all need to provide an additional power source to drive the sample flow, which is not suitable for applications in remote areas far from hospitals

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