2results about How to "Reduce matrix effect" patented technology

This invention discloses a method for detecting bromofenac in ginger, belonging to the field of pesticide residue detection technology. The method includes: homogenizing and extracting ginger samples using an acetonitrile solution containing a hydrophobic deep eutectic solvent; adding a salting-out agent to obtain a supernatant through separation; purifying the supernatant using a boric acid-functionalized magnetic carbon nanotube metal-organic framework composite material as an adsorbent via a temperature-switching adsorption process, first removing phenolic interference through thermal adsorption, then removing lipids through freeze precipitation; and finally detecting the supernatant using liquid chromatography-tandem mass spectrometry. This invention effectively solves the problem of severe matrix interference from phenols and lipids in ginger, and features high recovery rate, low matrix effect, high sensitivity, and simple operation.

The application discloses an artificial urine buffer matrix and a preparation method and application thereof. The artificial urine buffer matrix comprises urea, uric acid, alpha-hydroxy acid, amino acid, ions and sugar. The artificial urine buffer matrix can eliminate background interference, guarantee the immune reaction detection of small molecules, proteins and other biomarkers in urine, and has small matrix effect.